AlfalfaGEDB | Msa0311100

M. sativa cultivar zhongmu-4 / Msa0311100

KEGG: -
GO: -
PFAM: PF12734.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311100	ABN05724.1	100.000	69	0	0	1	69	1	69	4.32e-39	133

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311100	A2Q1A7	100.000	69	0	0	1	69	1	69	2.06e-39	133

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0050730	Msa0311100	0.805397	1.361374e-49	-8.615850e-47
Msa0050760	Msa0311100	0.800758	1.240342e-48	-8.615850e-47
Msa0190360	Msa0311100	0.918717	1.103524e-86	-8.615850e-47
Msa0272000	Msa0311100	0.865480	5.728357e-65	-8.615850e-47
Msa0311100	Msa0908170	0.805738	1.154607e-49	-8.615850e-47
Msa0311100	Msa1404540	0.803651	3.149386e-49	-8.615850e-47
Msa0311100	Msa1431060	0.800833	1.197063e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311100	MtrunA17_Chr2g0279941	100.000	69	0	0	1	69	1	69	3.98e-43	133

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 32 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATAACCAAATATATA+TGG	0.338136	2_4:-5978313	Msa0311100:three_prime_UTR
AAAGAAAAGGAAGATAATAA+AGG	0.362142	2_4:+5978338	None:intergenic
AACAGCAACACAAAGCTGCT+AGG	0.601258	2_4:+5978588	None:intergenic
AAGAAAAGGAAGATAATAAA+GGG	0.377219	2_4:+5978339	None:intergenic
ACCGGAGGTGGTCCTTGATA+AGG	0.377579	2_4:+5978815	None:intergenic
AGAAAAGGAAGATAATAAAG+GGG	0.613618	2_4:+5978340	None:intergenic
AGCTTTCTGCCATATATATT+TGG	0.170915	2_4:+5978304	None:intergenic
AGTACATTTATTATCTGAAA+GGG	0.466301	2_4:-5978367	Msa0311100:three_prime_UTR
ATATTCCACAACTTTAATAA+TGG	0.294529	2_4:+5978241	None:intergenic
ATGGTGTATGTACCTTGAGC+AGG	0.537878	2_4:+5979011	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATACTCATACACATGCTTT+AGG	+	chr2_4:5978228-5978247	None:intergenic	30.0%
CAATTATACACTATATAGGG+TGG	-	chr2_4:5978666-5978685	Msa0311100:intron	30.0%
AGCTCGCAAAAATAATAGTT+GGG	+	chr2_4:5978844-5978863	None:intergenic	30.0%
TAGCTCGCAAAAATAATAGT+TGG	+	chr2_4:5978845-5978864	None:intergenic	30.0%
AGCTTTCTGCCATATATATT+TGG	+	chr2_4:5979080-5979099	None:intergenic	30.0%
AAAATGGAGAGGGTAAAAAA+TGG	+	chr2_4:5979118-5979137	None:intergenic	30.0%
GGAATATAGATACAATAACG+TGG	-	chr2_4:5979156-5979175	Msa0311100:five_prime_UTR	30.0%
TATACTCATACACATGCTTT+AGG	+	chr2_4:5978228-5978247	None:intergenic	30.0%
CAATTATACACTATATAGGG+TGG	-	chr2_4:5978666-5978685	Msa0311100:intron	30.0%
AGCTCGCAAAAATAATAGTT+GGG	+	chr2_4:5978844-5978863	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	5978225	5979178	5978225	ID=Msa0311100;Name=Msa0311100
chr2_4	mRNA	5978225	5979178	5978225	ID=Msa0311100-mRNA-1;Parent=Msa0311100;Name=Msa0311100-mRNA-1;_AED=0.00;_eAED=0.00;_QI=115\|1\|1\|1\|1\|1\|3\|313\|69
chr2_4	exon	5978225	5978610	5978225	ID=Msa0311100-mRNA-1:exon:2254;Parent=Msa0311100-mRNA-1
chr2_4	exon	5978743	5978839	5978743	ID=Msa0311100-mRNA-1:exon:2253;Parent=Msa0311100-mRNA-1
chr2_4	exon	5979024	5979178	5979024	ID=Msa0311100-mRNA-1:exon:2252;Parent=Msa0311100-mRNA-1
chr2_4	five_prime_UTR	5979064	5979178	5979064	ID=Msa0311100-mRNA-1:five_prime_utr;Parent=Msa0311100-mRNA-1
chr2_4	CDS	5979024	5979063	5979024	ID=Msa0311100-mRNA-1:cds;Parent=Msa0311100-mRNA-1
chr2_4	CDS	5978743	5978839	5978743	ID=Msa0311100-mRNA-1:cds;Parent=Msa0311100-mRNA-1
chr2_4	CDS	5978538	5978610	5978538	ID=Msa0311100-mRNA-1:cds;Parent=Msa0311100-mRNA-1
chr2_4	three_prime_UTR	5978225	5978537	5978225	ID=Msa0311100-mRNA-1:three_prime_utr;Parent=Msa0311100-mRNA-1

Gene Sequence	>Msa0311100 ATGAGTGAGATCAAATATGGCTATCCATATCCTGCTCAAGGCCCTTATCAAGGACCACCTCCGGTGGCGGCACCACCGCAATACTATGCTGCTCCACCACCACCACCAAAAAGAGAGCCGGGTTTTCTTGAGGGATGCCTAGCAGCTTTGTGTTGCTGTTGCCTCTTGGATGAGTGCTGCTGTGACCCAACTATTATTTTTGCGAGCTAA
Protein sequence	>Msa0311100 MSEIKYGYPYPAQGPYQGPPPVAAPPQYYAAPPPPPKREPGFLEGCLAALCCCCLLDECCCDPTIIFAS*