AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0311100


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0311100 MtrunA17_Chr2g0279941 100.000 69 0 0 1 69 1 69 3.98e-43 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTTCTGCCATATATATT+TGG 0.170915 2_4:+5978304 None:intergenic
ATATTCCACAACTTTAATAA+TGG 0.294529 2_4:+5978241 None:intergenic
TATACTCATACACATGCTTT+AGG 0.317198 2_4:+5979156 None:intergenic
AAAATATAACCAAATATATA+TGG 0.338136 2_4:-5978313 Msa0311100:three_prime_UTR
AAAGAAAAGGAAGATAATAA+AGG 0.362142 2_4:+5978338 None:intergenic
TACTCACTCATACAATTAAT+AGG 0.364891 2_4:+5978424 None:intergenic
AAGAAAAGGAAGATAATAAA+GGG 0.377219 2_4:+5978339 None:intergenic
ACCGGAGGTGGTCCTTGATA+AGG 0.377579 2_4:+5978815 None:intergenic
GCTAATGCATTTCACCAAAC+TGG 0.387821 2_4:-5978520 Msa0311100:exon
TATCATATAATATCAAATAT+TGG 0.395323 2_4:+5978396 None:intergenic
GAGATGAGTGAGATCAAATA+TGG 0.409886 2_4:-5979044 Msa0311100:CDS
TATGTACCTTGAGCAGGATA+TGG 0.426594 2_4:+5979017 None:intergenic
TTTGTGTTGCTGTTGCCTCT+TGG 0.433493 2_4:-5978579 Msa0311100:CDS
GGTGGTGCCGCCACCGGAGG+TGG 0.452814 2_4:+5978803 None:intergenic
CCGGAGGTGGTCCTTGATAA+GGG 0.453222 2_4:+5978816 None:intergenic
AGTACATTTATTATCTGAAA+GGG 0.466301 2_4:-5978367 Msa0311100:three_prime_UTR
CCCTTATCAAGGACCACCTC+CGG 0.501946 2_4:-5978816 Msa0311100:CDS
TTCATACTAGGCCCTTATCA+AGG 0.506361 2_4:-5978827 Msa0311100:intron
TCAAGGACCACCTCCGGTGG+CGG 0.518251 2_4:-5978810 Msa0311100:CDS
ATGGTGTATGTACCTTGAGC+AGG 0.537878 2_4:+5979011 None:intergenic
TGCGGTGGTGCCGCCACCGG+AGG 0.538797 2_4:+5978800 None:intergenic
TATTGCGGTGGTGCCGCCAC+CGG 0.539716 2_4:+5978797 None:intergenic
TAGTACATTTATTATCTGAA+AGG 0.542095 2_4:-5978368 Msa0311100:three_prime_UTR
GGCTATCCATATCCTGCTCA+AGG 0.571306 2_4:-5979023 Msa0311100:intron
TACCATCCCTCAAGAAAACC+CGG 0.577880 2_4:+5978740 None:intergenic
AACAGCAACACAAAGCTGCT+AGG 0.601258 2_4:+5978588 None:intergenic
AGAAAAGGAAGATAATAAAG+GGG 0.613618 2_4:+5978340 None:intergenic
TTATCAAGGACCACCTCCGG+TGG 0.637488 2_4:-5978813 Msa0311100:CDS
CACAGCAGCACTCATCCAAG+AGG 0.685776 2_4:+5978564 None:intergenic
GGAGCAGCATAGTATTGCGG+TGG 0.697423 2_4:+5978785 None:intergenic
GGTGGAGCAGCATAGTATTG+CGG 0.699649 2_4:+5978782 None:intergenic
GGAATATAGATACAATAACG+TGG 0.751387 2_4:-5978225 Msa0311100:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTCAAGAATAATTTT+AGG - chr2_4:5978293-5978312 Msa0311100:three_prime_UTR 10.0%
!! TATCATATAATATCAAATAT+TGG + chr2_4:5978988-5979007 None:intergenic 10.0%
!! AAAATATAACCAAATATATA+TGG - chr2_4:5979068-5979087 Msa0311100:five_prime_UTR 10.0%
!!! TTTATTTCAAGAATAATTTT+AGG - chr2_4:5978293-5978312 Msa0311100:three_prime_UTR 10.0%
!! TATCATATAATATCAAATAT+TGG + chr2_4:5978988-5979007 None:intergenic 10.0%
!! AAAATATAACCAAATATATA+TGG - chr2_4:5979068-5979087 Msa0311100:five_prime_UTR 10.0%
!!! AAGTTTTAGTATATAATTTG+AGG + chr2_4:5978414-5978433 None:intergenic 15.0%
!! GTTTCAATATTGTAATAAAA+AGG + chr2_4:5978454-5978473 None:intergenic 15.0%
!! AAAAAAAATACATTGTTTAG+TGG - chr2_4:5978928-5978947 Msa0311100:intron 15.0%
!!! GGTTATATTTTTAAAAGAAA+AGG + chr2_4:5979059-5979078 None:intergenic 15.0%
!!! AACTTTAATAATGGAAAAAA+TGG + chr2_4:5979134-5979153 None:intergenic 15.0%
!!! AAGTTTTAGTATATAATTTG+AGG + chr2_4:5978414-5978433 None:intergenic 15.0%
!! GTTTCAATATTGTAATAAAA+AGG + chr2_4:5978454-5978473 None:intergenic 15.0%
!! AAAAAAAATACATTGTTTAG+TGG - chr2_4:5978928-5978947 Msa0311100:intron 15.0%
!!! GGTTATATTTTTAAAAGAAA+AGG + chr2_4:5979059-5979078 None:intergenic 15.0%
!!! AACTTTAATAATGGAAAAAA+TGG + chr2_4:5979134-5979153 None:intergenic 15.0%
!! GAATGAGAAATATGTATATA+TGG + chr2_4:5978392-5978411 None:intergenic 20.0%
!!! TTGTTGTTATATTTCATACT+AGG - chr2_4:5978542-5978561 Msa0311100:CDS 20.0%
!! TAAGCAATTATACACTATAT+AGG - chr2_4:5978662-5978681 Msa0311100:intron 20.0%
!! AAGCAATTATACACTATATA+GGG - chr2_4:5978663-5978682 Msa0311100:intron 20.0%
!! TAGTACATTTATTATCTGAA+AGG - chr2_4:5979013-5979032 Msa0311100:intron 20.0%
!! AGTACATTTATTATCTGAAA+GGG - chr2_4:5979014-5979033 Msa0311100:intron 20.0%
!! AAGAAAAGGAAGATAATAAA+GGG + chr2_4:5979045-5979064 None:intergenic 20.0%
!! AAAGAAAAGGAAGATAATAA+AGG + chr2_4:5979046-5979065 None:intergenic 20.0%
!!! TTTTTCCATTATTAAAGTTG+TGG - chr2_4:5979135-5979154 Msa0311100:five_prime_UTR 20.0%
!! ATATTCCACAACTTTAATAA+TGG + chr2_4:5979143-5979162 None:intergenic 20.0%
!! GAATGAGAAATATGTATATA+TGG + chr2_4:5978392-5978411 None:intergenic 20.0%
!!! TTGTTGTTATATTTCATACT+AGG - chr2_4:5978542-5978561 Msa0311100:CDS 20.0%
!! TAAGCAATTATACACTATAT+AGG - chr2_4:5978662-5978681 Msa0311100:intron 20.0%
!! AAGCAATTATACACTATATA+GGG - chr2_4:5978663-5978682 Msa0311100:intron 20.0%
!! TAGTACATTTATTATCTGAA+AGG - chr2_4:5979013-5979032 Msa0311100:intron 20.0%
!! AGTACATTTATTATCTGAAA+GGG - chr2_4:5979014-5979033 Msa0311100:intron 20.0%
!! AAGAAAAGGAAGATAATAAA+GGG + chr2_4:5979045-5979064 None:intergenic 20.0%
!! AAAGAAAAGGAAGATAATAA+AGG + chr2_4:5979046-5979065 None:intergenic 20.0%
!!! TTTTTCCATTATTAAAGTTG+TGG - chr2_4:5979135-5979154 Msa0311100:five_prime_UTR 20.0%
!! ATATTCCACAACTTTAATAA+TGG + chr2_4:5979143-5979162 None:intergenic 20.0%
!! AAGAACTTGTAATTTTGTGT+TGG + chr2_4:5978274-5978293 None:intergenic 25.0%
! TACTCACTCATACAATTAAT+AGG + chr2_4:5978960-5978979 None:intergenic 25.0%
! AGAAAAGGAAGATAATAAAG+GGG + chr2_4:5979044-5979063 None:intergenic 25.0%
!! AATAATGGAAAAAATGGAGA+GGG + chr2_4:5979128-5979147 None:intergenic 25.0%
!! TAATAATGGAAAAAATGGAG+AGG + chr2_4:5979129-5979148 None:intergenic 25.0%
!! AAGAACTTGTAATTTTGTGT+TGG + chr2_4:5978274-5978293 None:intergenic 25.0%
! TACTCACTCATACAATTAAT+AGG + chr2_4:5978960-5978979 None:intergenic 25.0%
! AGAAAAGGAAGATAATAAAG+GGG + chr2_4:5979044-5979063 None:intergenic 25.0%
!! AATAATGGAAAAAATGGAGA+GGG + chr2_4:5979128-5979147 None:intergenic 25.0%
!! TAATAATGGAAAAAATGGAG+AGG + chr2_4:5979129-5979148 None:intergenic 25.0%
TATACTCATACACATGCTTT+AGG + chr2_4:5978228-5978247 None:intergenic 30.0%
! TAATTTTGTGTTGGAATGCA+GGG + chr2_4:5978265-5978284 None:intergenic 30.0%
CAATTATACACTATATAGGG+TGG - chr2_4:5978666-5978685 Msa0311100:intron 30.0%
AGCTCGCAAAAATAATAGTT+GGG + chr2_4:5978844-5978863 None:intergenic 30.0%
TAGCTCGCAAAAATAATAGT+TGG + chr2_4:5978845-5978864 None:intergenic 30.0%
AGCTTTCTGCCATATATATT+TGG + chr2_4:5979080-5979099 None:intergenic 30.0%
AAAATGGAGAGGGTAAAAAA+TGG + chr2_4:5979118-5979137 None:intergenic 30.0%
GGAATATAGATACAATAACG+TGG - chr2_4:5979156-5979175 Msa0311100:five_prime_UTR 30.0%
TATACTCATACACATGCTTT+AGG + chr2_4:5978228-5978247 None:intergenic 30.0%
! TAATTTTGTGTTGGAATGCA+GGG + chr2_4:5978265-5978284 None:intergenic 30.0%
CAATTATACACTATATAGGG+TGG - chr2_4:5978666-5978685 Msa0311100:intron 30.0%
AGCTCGCAAAAATAATAGTT+GGG + chr2_4:5978844-5978863 None:intergenic 30.0%
TAGCTCGCAAAAATAATAGT+TGG + chr2_4:5978845-5978864 None:intergenic 30.0%
AGCTTTCTGCCATATATATT+TGG + chr2_4:5979080-5979099 None:intergenic 30.0%
AAAATGGAGAGGGTAAAAAA+TGG + chr2_4:5979118-5979137 None:intergenic 30.0%
! GTAATTTTGTGTTGGAATGC+AGG + chr2_4:5978266-5978285 None:intergenic 35.0%
GAGATGAGTGAGATCAAATA+TGG - chr2_4:5978337-5978356 Msa0311100:three_prime_UTR 35.0%
! CAATAGCTTCTTTTCCAGTT+TGG + chr2_4:5978878-5978897 None:intergenic 35.0%
! GTAATTTTGTGTTGGAATGC+AGG + chr2_4:5978266-5978285 None:intergenic 35.0%
GAGATGAGTGAGATCAAATA+TGG - chr2_4:5978337-5978356 Msa0311100:three_prime_UTR 35.0%
! CAATAGCTTCTTTTCCAGTT+TGG + chr2_4:5978878-5978897 None:intergenic 35.0%
TATGTACCTTGAGCAGGATA+TGG + chr2_4:5978367-5978386 None:intergenic 40.0%
TTCATACTAGGCCCTTATCA+AGG - chr2_4:5978554-5978573 Msa0311100:CDS 40.0%
GCTAATGCATTTCACCAAAC+TGG - chr2_4:5978861-5978880 Msa0311100:intron 40.0%
TATGTACCTTGAGCAGGATA+TGG + chr2_4:5978367-5978386 None:intergenic 40.0%
TTCATACTAGGCCCTTATCA+AGG - chr2_4:5978554-5978573 Msa0311100:CDS 40.0%
GCTAATGCATTTCACCAAAC+TGG - chr2_4:5978861-5978880 Msa0311100:intron 40.0%
ATGGTGTATGTACCTTGAGC+AGG + chr2_4:5978373-5978392 None:intergenic 45.0%
! AGAAAACCCGGCTCTCTTTT+TGG + chr2_4:5978632-5978651 None:intergenic 45.0%
TACCATCCCTCAAGAAAACC+CGG + chr2_4:5978644-5978663 None:intergenic 45.0%
GCTGCTAGGCTGCATATAAT+TGG + chr2_4:5978782-5978801 None:intergenic 45.0%
AACAGCAACACAAAGCTGCT+AGG + chr2_4:5978796-5978815 None:intergenic 45.0%
! TTTGTGTTGCTGTTGCCTCT+TGG - chr2_4:5978802-5978821 Msa0311100:CDS 45.0%
ATGGTGTATGTACCTTGAGC+AGG + chr2_4:5978373-5978392 None:intergenic 45.0%
! AGAAAACCCGGCTCTCTTTT+TGG + chr2_4:5978632-5978651 None:intergenic 45.0%
TACCATCCCTCAAGAAAACC+CGG + chr2_4:5978644-5978663 None:intergenic 45.0%
GCTGCTAGGCTGCATATAAT+TGG + chr2_4:5978782-5978801 None:intergenic 45.0%
AACAGCAACACAAAGCTGCT+AGG + chr2_4:5978796-5978815 None:intergenic 45.0%
! TTTGTGTTGCTGTTGCCTCT+TGG - chr2_4:5978802-5978821 Msa0311100:CDS 45.0%
GGCTATCCATATCCTGCTCA+AGG - chr2_4:5978358-5978377 Msa0311100:three_prime_UTR 50.0%
GGTGGAGCAGCATAGTATTG+CGG + chr2_4:5978602-5978621 None:intergenic 50.0%
!!! TCTCTTTTTGGTGGTGGTGG+TGG + chr2_4:5978620-5978639 None:intergenic 50.0%
ACCACCACCAAAAAGAGAGC+CGG - chr2_4:5978622-5978641 Msa0311100:intron 50.0%
! AAACCCGGCTCTCTTTTTGG+TGG + chr2_4:5978629-5978648 None:intergenic 50.0%
! AAGAGAGCCGGGTTTTCTTG+AGG - chr2_4:5978634-5978653 Msa0311100:intron 50.0%
! AGAGAGCCGGGTTTTCTTGA+GGG - chr2_4:5978635-5978654 Msa0311100:intron 50.0%
GGCTATCCATATCCTGCTCA+AGG - chr2_4:5978358-5978377 Msa0311100:three_prime_UTR 50.0%
GGTGGAGCAGCATAGTATTG+CGG + chr2_4:5978602-5978621 None:intergenic 50.0%
!!! TCTCTTTTTGGTGGTGGTGG+TGG + chr2_4:5978620-5978639 None:intergenic 50.0%
ACCACCACCAAAAAGAGAGC+CGG - chr2_4:5978622-5978641 Msa0311100:intron 50.0%
! AAACCCGGCTCTCTTTTTGG+TGG + chr2_4:5978629-5978648 None:intergenic 50.0%
! AAGAGAGCCGGGTTTTCTTG+AGG - chr2_4:5978634-5978653 Msa0311100:intron 50.0%
! AGAGAGCCGGGTTTTCTTGA+GGG - chr2_4:5978635-5978654 Msa0311100:intron 50.0%
CCGGAGGTGGTCCTTGATAA+GGG + chr2_4:5978568-5978587 None:intergenic 55.0%
CCCTTATCAAGGACCACCTC+CGG - chr2_4:5978565-5978584 Msa0311100:CDS 55.0%
ACCGGAGGTGGTCCTTGATA+AGG + chr2_4:5978569-5978588 None:intergenic 55.0%
TTATCAAGGACCACCTCCGG+TGG - chr2_4:5978568-5978587 Msa0311100:CDS 55.0%
GGAGCAGCATAGTATTGCGG+TGG + chr2_4:5978599-5978618 None:intergenic 55.0%
!!! GGCTCTCTTTTTGGTGGTGG+TGG + chr2_4:5978623-5978642 None:intergenic 55.0%
CCACCACCAAAAAGAGAGCC+GGG - chr2_4:5978623-5978642 Msa0311100:intron 55.0%
! AGCCGGGTTTTCTTGAGGGA+TGG - chr2_4:5978639-5978658 Msa0311100:intron 55.0%
CACAGCAGCACTCATCCAAG+AGG + chr2_4:5978820-5978839 None:intergenic 55.0%
CCGGAGGTGGTCCTTGATAA+GGG + chr2_4:5978568-5978587 None:intergenic 55.0%
CCCTTATCAAGGACCACCTC+CGG - chr2_4:5978565-5978584 Msa0311100:CDS 55.0%
ACCGGAGGTGGTCCTTGATA+AGG + chr2_4:5978569-5978588 None:intergenic 55.0%
TTATCAAGGACCACCTCCGG+TGG - chr2_4:5978568-5978587 Msa0311100:CDS 55.0%
GGAGCAGCATAGTATTGCGG+TGG + chr2_4:5978599-5978618 None:intergenic 55.0%
!!! GGCTCTCTTTTTGGTGGTGG+TGG + chr2_4:5978623-5978642 None:intergenic 55.0%
CCACCACCAAAAAGAGAGCC+GGG - chr2_4:5978623-5978642 Msa0311100:intron 55.0%
! AGCCGGGTTTTCTTGAGGGA+TGG - chr2_4:5978639-5978658 Msa0311100:intron 55.0%
CACAGCAGCACTCATCCAAG+AGG + chr2_4:5978820-5978839 None:intergenic 55.0%
!! CCCGGCTCTCTTTTTGGTGG+TGG + chr2_4:5978626-5978645 None:intergenic 60.0%
!! CCCGGCTCTCTTTTTGGTGG+TGG + chr2_4:5978626-5978645 None:intergenic 60.0%
TCAAGGACCACCTCCGGTGG+CGG - chr2_4:5978571-5978590 Msa0311100:CDS 65.0%
!! TATTGCGGTGGTGCCGCCAC+CGG + chr2_4:5978587-5978606 None:intergenic 65.0%
TCAAGGACCACCTCCGGTGG+CGG - chr2_4:5978571-5978590 Msa0311100:CDS 65.0%
!! TATTGCGGTGGTGCCGCCAC+CGG + chr2_4:5978587-5978606 None:intergenic 65.0%
!!! GGTGGTGCCGCCACCGGAGG+TGG + chr2_4:5978581-5978600 None:intergenic 80.0%
!!! TGCGGTGGTGCCGCCACCGG+AGG + chr2_4:5978584-5978603 None:intergenic 80.0%
!!! GGTGGTGCCGCCACCGGAGG+TGG + chr2_4:5978581-5978600 None:intergenic 80.0%
!!! TGCGGTGGTGCCGCCACCGG+AGG + chr2_4:5978584-5978603 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 5978225 5979178 5978225 ID=Msa0311100;Name=Msa0311100
chr2_4 mRNA 5978225 5979178 5978225 ID=Msa0311100-mRNA-1;Parent=Msa0311100;Name=Msa0311100-mRNA-1;_AED=0.00;_eAED=0.00;_QI=115|1|1|1|1|1|3|313|69
chr2_4 exon 5978225 5978610 5978225 ID=Msa0311100-mRNA-1:exon:2254;Parent=Msa0311100-mRNA-1
chr2_4 exon 5978743 5978839 5978743 ID=Msa0311100-mRNA-1:exon:2253;Parent=Msa0311100-mRNA-1
chr2_4 exon 5979024 5979178 5979024 ID=Msa0311100-mRNA-1:exon:2252;Parent=Msa0311100-mRNA-1
chr2_4 five_prime_UTR 5979064 5979178 5979064 ID=Msa0311100-mRNA-1:five_prime_utr;Parent=Msa0311100-mRNA-1
chr2_4 CDS 5979024 5979063 5979024 ID=Msa0311100-mRNA-1:cds;Parent=Msa0311100-mRNA-1
chr2_4 CDS 5978743 5978839 5978743 ID=Msa0311100-mRNA-1:cds;Parent=Msa0311100-mRNA-1
chr2_4 CDS 5978538 5978610 5978538 ID=Msa0311100-mRNA-1:cds;Parent=Msa0311100-mRNA-1
chr2_4 three_prime_UTR 5978225 5978537 5978225 ID=Msa0311100-mRNA-1:three_prime_utr;Parent=Msa0311100-mRNA-1
Gene Sequence

>Msa0311100

ATGAGTGAGATCAAATATGGCTATCCATATCCTGCTCAAGGCCCTTATCAAGGACCACCTCCGGTGGCGGCACCACCGCAATACTATGCTGCTCCACCACCACCACCAAAAAGAGAGCCGGGTTTTCTTGAGGGATGCCTAGCAGCTTTGTGTTGCTGTTGCCTCTTGGATGAGTGCTGCTGTGACCCAACTATTATTTTTGCGAGCTAA

Protein sequence

>Msa0311100

MSEIKYGYPYPAQGPYQGPPPVAAPPQYYAAPPPPPKREPGFLEGCLAALCCCCLLDECCCDPTIIFAS*