AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0190360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0190360 MtrunA17_Chr2g0279941 100.000 69 0 0 1 69 1 69 3.98e-43 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTTCTGCCATATATATT+TGG 0.170915 2_1:+5873257 None:intergenic
ATATTCCACAACTTTAATAA+TGG 0.294529 2_1:+5873194 None:intergenic
TATACTCATACACATGCTTT+AGG 0.317198 2_1:+5874115 None:intergenic
AAAATATAACCAAATATATA+TGG 0.338136 2_1:-5873266 Msa0190360:three_prime_UTR
AAAGAAAAGGAAGATAATAA+AGG 0.362142 2_1:+5873291 None:intergenic
TACTCACTCATACAATTAAT+AGG 0.364891 2_1:+5873377 None:intergenic
AAGAAAAGGAAGATAATAAA+GGG 0.377219 2_1:+5873292 None:intergenic
ACCGGAGGTGGTCCTTGATA+AGG 0.377579 2_1:+5873774 None:intergenic
GCTAATGCATTTCACCAAAC+TGG 0.387821 2_1:-5873479 Msa0190360:exon
TATCATATAATATCAAATAT+TGG 0.395323 2_1:+5873349 None:intergenic
GAGATGAGTGAGATCAAATA+TGG 0.409886 2_1:-5874003 Msa0190360:CDS
TATGTACCTTGAGCAGGATA+TGG 0.426594 2_1:+5873976 None:intergenic
TTTGTGTTGCTGTTGCCTCT+TGG 0.433493 2_1:-5873538 Msa0190360:CDS
GGTGGTGCCGCCACCGGAGG+TGG 0.452814 2_1:+5873762 None:intergenic
CCGGAGGTGGTCCTTGATAA+GGG 0.453222 2_1:+5873775 None:intergenic
AGTACATTTATTATCTGAAA+GGG 0.466301 2_1:-5873320 Msa0190360:three_prime_UTR
CCCTTATCAAGGACCACCTC+CGG 0.501946 2_1:-5873775 Msa0190360:CDS
TTCATACTAGGCCCTTATCA+AGG 0.506361 2_1:-5873786 Msa0190360:intron
TCAAGGACCACCTCCGGTGG+CGG 0.518251 2_1:-5873769 Msa0190360:CDS
ATGGTGTATGTACCTTGAGC+AGG 0.537878 2_1:+5873970 None:intergenic
TGCGGTGGTGCCGCCACCGG+AGG 0.538797 2_1:+5873759 None:intergenic
TATTGCGGTGGTGCCGCCAC+CGG 0.539716 2_1:+5873756 None:intergenic
TAGTACATTTATTATCTGAA+AGG 0.542095 2_1:-5873321 Msa0190360:three_prime_UTR
GGCTATCCATATCCTGCTCA+AGG 0.571306 2_1:-5873982 Msa0190360:intron
TACCATCCCTCAAGAAAACC+CGG 0.577880 2_1:+5873699 None:intergenic
AACAGCAACACAAAGCTGCT+AGG 0.601258 2_1:+5873547 None:intergenic
AGAAAAGGAAGATAATAAAG+GGG 0.613618 2_1:+5873293 None:intergenic
TTATCAAGGACCACCTCCGG+TGG 0.637488 2_1:-5873772 Msa0190360:CDS
CACAGCAGCACTCATCCAAG+AGG 0.685776 2_1:+5873523 None:intergenic
GGAGCAGCATAGTATTGCGG+TGG 0.697423 2_1:+5873744 None:intergenic
GGTGGAGCAGCATAGTATTG+CGG 0.699649 2_1:+5873741 None:intergenic
GGAATATAGATACAATAACG+TGG 0.751387 2_1:-5873178 Msa0190360:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTCAAGAATAATTTT+AGG - chr2_1:5873246-5873265 Msa0190360:three_prime_UTR 10.0%
!! TATCATATAATATCAAATAT+TGG + chr2_1:5873947-5873966 None:intergenic 10.0%
!! AAAATATAACCAAATATATA+TGG - chr2_1:5874027-5874046 Msa0190360:five_prime_UTR 10.0%
!!! TTTATTTCAAGAATAATTTT+AGG - chr2_1:5873246-5873265 Msa0190360:three_prime_UTR 10.0%
!! TATCATATAATATCAAATAT+TGG + chr2_1:5873947-5873966 None:intergenic 10.0%
!! AAAATATAACCAAATATATA+TGG - chr2_1:5874027-5874046 Msa0190360:five_prime_UTR 10.0%
!!! AAGTTTTAGTATATAATTTG+AGG + chr2_1:5873367-5873386 None:intergenic 15.0%
!! GTTTCAATATTGTAATAAAA+AGG + chr2_1:5873407-5873426 None:intergenic 15.0%
!! AAAAAAAATACATTGTTTAG+TGG - chr2_1:5873887-5873906 Msa0190360:intron 15.0%
!!! GGTTATATTTTTAAAAGAAA+AGG + chr2_1:5874018-5874037 None:intergenic 15.0%
!!! AACTTTAATAATGGAAAAAA+TGG + chr2_1:5874093-5874112 None:intergenic 15.0%
!!! AAGTTTTAGTATATAATTTG+AGG + chr2_1:5873367-5873386 None:intergenic 15.0%
!! GTTTCAATATTGTAATAAAA+AGG + chr2_1:5873407-5873426 None:intergenic 15.0%
!! AAAAAAAATACATTGTTTAG+TGG - chr2_1:5873887-5873906 Msa0190360:intron 15.0%
!!! GGTTATATTTTTAAAAGAAA+AGG + chr2_1:5874018-5874037 None:intergenic 15.0%
!!! AACTTTAATAATGGAAAAAA+TGG + chr2_1:5874093-5874112 None:intergenic 15.0%
!!! AAGAACTTGTAATTTTATGT+TGG + chr2_1:5873227-5873246 None:intergenic 20.0%
!! GAATGAGAAATATGTATATA+TGG + chr2_1:5873345-5873364 None:intergenic 20.0%
!!! TTGTTGTTATATTTCATACT+AGG - chr2_1:5873495-5873514 Msa0190360:exon 20.0%
!! TAAGCAATTATACACTATAT+AGG - chr2_1:5873615-5873634 Msa0190360:intron 20.0%
!! AAGCAATTATACACTATATA+GGG - chr2_1:5873616-5873635 Msa0190360:intron 20.0%
!! TAGTACATTTATTATCTGAA+AGG - chr2_1:5873972-5873991 Msa0190360:intron 20.0%
!! AGTACATTTATTATCTGAAA+GGG - chr2_1:5873973-5873992 Msa0190360:intron 20.0%
!! AAGAAAAGGAAGATAATAAA+GGG + chr2_1:5874004-5874023 None:intergenic 20.0%
!! AAAGAAAAGGAAGATAATAA+AGG + chr2_1:5874005-5874024 None:intergenic 20.0%
!!! TTTTTCCATTATTAAAGTTG+TGG - chr2_1:5874094-5874113 Msa0190360:five_prime_UTR 20.0%
!! ATATTCCACAACTTTAATAA+TGG + chr2_1:5874102-5874121 None:intergenic 20.0%
!!! AAGAACTTGTAATTTTATGT+TGG + chr2_1:5873227-5873246 None:intergenic 20.0%
!! GAATGAGAAATATGTATATA+TGG + chr2_1:5873345-5873364 None:intergenic 20.0%
!!! TTGTTGTTATATTTCATACT+AGG - chr2_1:5873495-5873514 Msa0190360:exon 20.0%
!! TAAGCAATTATACACTATAT+AGG - chr2_1:5873615-5873634 Msa0190360:intron 20.0%
!! AAGCAATTATACACTATATA+GGG - chr2_1:5873616-5873635 Msa0190360:intron 20.0%
!! TAGTACATTTATTATCTGAA+AGG - chr2_1:5873972-5873991 Msa0190360:intron 20.0%
!! AGTACATTTATTATCTGAAA+GGG - chr2_1:5873973-5873992 Msa0190360:intron 20.0%
!! AAGAAAAGGAAGATAATAAA+GGG + chr2_1:5874004-5874023 None:intergenic 20.0%
!! AAAGAAAAGGAAGATAATAA+AGG + chr2_1:5874005-5874024 None:intergenic 20.0%
!!! TTTTTCCATTATTAAAGTTG+TGG - chr2_1:5874094-5874113 Msa0190360:five_prime_UTR 20.0%
!! ATATTCCACAACTTTAATAA+TGG + chr2_1:5874102-5874121 None:intergenic 20.0%
!! TAATTTTATGTTGGAATGCA+GGG + chr2_1:5873218-5873237 None:intergenic 25.0%
! TACTCACTCATACAATTAAT+AGG + chr2_1:5873919-5873938 None:intergenic 25.0%
! AGAAAAGGAAGATAATAAAG+GGG + chr2_1:5874003-5874022 None:intergenic 25.0%
!! AATAATGGAAAAAATGGAGA+GGG + chr2_1:5874087-5874106 None:intergenic 25.0%
!! TAATAATGGAAAAAATGGAG+AGG + chr2_1:5874088-5874107 None:intergenic 25.0%
!! TAATTTTATGTTGGAATGCA+GGG + chr2_1:5873218-5873237 None:intergenic 25.0%
! TACTCACTCATACAATTAAT+AGG + chr2_1:5873919-5873938 None:intergenic 25.0%
! AGAAAAGGAAGATAATAAAG+GGG + chr2_1:5874003-5874022 None:intergenic 25.0%
!! AATAATGGAAAAAATGGAGA+GGG + chr2_1:5874087-5874106 None:intergenic 25.0%
!! TAATAATGGAAAAAATGGAG+AGG + chr2_1:5874088-5874107 None:intergenic 25.0%
TATACTCATACACATGCTTT+AGG + chr2_1:5873181-5873200 None:intergenic 30.0%
! GTAATTTTATGTTGGAATGC+AGG + chr2_1:5873219-5873238 None:intergenic 30.0%
CAATTATACACTATATAGGG+TGG - chr2_1:5873619-5873638 Msa0190360:intron 30.0%
AGCTCGCAAAAATAATAGTT+GGG + chr2_1:5873797-5873816 None:intergenic 30.0%
TAGCTCGCAAAAATAATAGT+TGG + chr2_1:5873798-5873817 None:intergenic 30.0%
AGCTTTCTGCCATATATATT+TGG + chr2_1:5874039-5874058 None:intergenic 30.0%
AAAATGGAGAGGGTAAAAAA+TGG + chr2_1:5874077-5874096 None:intergenic 30.0%
GGAATATAGATACAATAACG+TGG - chr2_1:5874115-5874134 Msa0190360:five_prime_UTR 30.0%
TATACTCATACACATGCTTT+AGG + chr2_1:5873181-5873200 None:intergenic 30.0%
! GTAATTTTATGTTGGAATGC+AGG + chr2_1:5873219-5873238 None:intergenic 30.0%
CAATTATACACTATATAGGG+TGG - chr2_1:5873619-5873638 Msa0190360:intron 30.0%
AGCTCGCAAAAATAATAGTT+GGG + chr2_1:5873797-5873816 None:intergenic 30.0%
TAGCTCGCAAAAATAATAGT+TGG + chr2_1:5873798-5873817 None:intergenic 30.0%
AGCTTTCTGCCATATATATT+TGG + chr2_1:5874039-5874058 None:intergenic 30.0%
AAAATGGAGAGGGTAAAAAA+TGG + chr2_1:5874077-5874096 None:intergenic 30.0%
GAGATGAGTGAGATCAAATA+TGG - chr2_1:5873290-5873309 Msa0190360:three_prime_UTR 35.0%
! CAATAGCTTCTTTTCCAGTT+TGG + chr2_1:5873831-5873850 None:intergenic 35.0%
GAGATGAGTGAGATCAAATA+TGG - chr2_1:5873290-5873309 Msa0190360:three_prime_UTR 35.0%
! CAATAGCTTCTTTTCCAGTT+TGG + chr2_1:5873831-5873850 None:intergenic 35.0%
TATGTACCTTGAGCAGGATA+TGG + chr2_1:5873320-5873339 None:intergenic 40.0%
TTCATACTAGGCCCTTATCA+AGG - chr2_1:5873507-5873526 Msa0190360:CDS 40.0%
CTGCTAGGCTGCATATAATT+GGG + chr2_1:5873734-5873753 None:intergenic 40.0%
GCTAATGCATTTCACCAAAC+TGG - chr2_1:5873814-5873833 Msa0190360:intron 40.0%
TATGTACCTTGAGCAGGATA+TGG + chr2_1:5873320-5873339 None:intergenic 40.0%
TTCATACTAGGCCCTTATCA+AGG - chr2_1:5873507-5873526 Msa0190360:CDS 40.0%
CTGCTAGGCTGCATATAATT+GGG + chr2_1:5873734-5873753 None:intergenic 40.0%
GCTAATGCATTTCACCAAAC+TGG - chr2_1:5873814-5873833 Msa0190360:intron 40.0%
ATGGTGTATGTACCTTGAGC+AGG + chr2_1:5873326-5873345 None:intergenic 45.0%
! AGAAAACCCGGCTCTCTTTT+TGG + chr2_1:5873585-5873604 None:intergenic 45.0%
TACCATCCCTCAAGAAAACC+CGG + chr2_1:5873597-5873616 None:intergenic 45.0%
GCTGCTAGGCTGCATATAAT+TGG + chr2_1:5873735-5873754 None:intergenic 45.0%
AACAGCAACACAAAGCTGCT+AGG + chr2_1:5873749-5873768 None:intergenic 45.0%
! TTTGTGTTGCTGTTGCCTCT+TGG - chr2_1:5873755-5873774 Msa0190360:CDS 45.0%
ATGGTGTATGTACCTTGAGC+AGG + chr2_1:5873326-5873345 None:intergenic 45.0%
! AGAAAACCCGGCTCTCTTTT+TGG + chr2_1:5873585-5873604 None:intergenic 45.0%
TACCATCCCTCAAGAAAACC+CGG + chr2_1:5873597-5873616 None:intergenic 45.0%
GCTGCTAGGCTGCATATAAT+TGG + chr2_1:5873735-5873754 None:intergenic 45.0%
AACAGCAACACAAAGCTGCT+AGG + chr2_1:5873749-5873768 None:intergenic 45.0%
! TTTGTGTTGCTGTTGCCTCT+TGG - chr2_1:5873755-5873774 Msa0190360:CDS 45.0%
GGCTATCCATATCCTGCTCA+AGG - chr2_1:5873311-5873330 Msa0190360:three_prime_UTR 50.0%
GGTGGAGCAGCATAGTATTG+CGG + chr2_1:5873555-5873574 None:intergenic 50.0%
!!! TCTCTTTTTGGTGGTGGTGG+TGG + chr2_1:5873573-5873592 None:intergenic 50.0%
ACCACCACCAAAAAGAGAGC+CGG - chr2_1:5873575-5873594 Msa0190360:intron 50.0%
! AAACCCGGCTCTCTTTTTGG+TGG + chr2_1:5873582-5873601 None:intergenic 50.0%
! AAGAGAGCCGGGTTTTCTTG+AGG - chr2_1:5873587-5873606 Msa0190360:intron 50.0%
! AGAGAGCCGGGTTTTCTTGA+GGG - chr2_1:5873588-5873607 Msa0190360:intron 50.0%
GGCTATCCATATCCTGCTCA+AGG - chr2_1:5873311-5873330 Msa0190360:three_prime_UTR 50.0%
GGTGGAGCAGCATAGTATTG+CGG + chr2_1:5873555-5873574 None:intergenic 50.0%
!!! TCTCTTTTTGGTGGTGGTGG+TGG + chr2_1:5873573-5873592 None:intergenic 50.0%
ACCACCACCAAAAAGAGAGC+CGG - chr2_1:5873575-5873594 Msa0190360:intron 50.0%
! AAACCCGGCTCTCTTTTTGG+TGG + chr2_1:5873582-5873601 None:intergenic 50.0%
! AAGAGAGCCGGGTTTTCTTG+AGG - chr2_1:5873587-5873606 Msa0190360:intron 50.0%
! AGAGAGCCGGGTTTTCTTGA+GGG - chr2_1:5873588-5873607 Msa0190360:intron 50.0%
CCGGAGGTGGTCCTTGATAA+GGG + chr2_1:5873521-5873540 None:intergenic 55.0%
CCCTTATCAAGGACCACCTC+CGG - chr2_1:5873518-5873537 Msa0190360:CDS 55.0%
ACCGGAGGTGGTCCTTGATA+AGG + chr2_1:5873522-5873541 None:intergenic 55.0%
TTATCAAGGACCACCTCCGG+TGG - chr2_1:5873521-5873540 Msa0190360:CDS 55.0%
GGAGCAGCATAGTATTGCGG+TGG + chr2_1:5873552-5873571 None:intergenic 55.0%
!!! GGCTCTCTTTTTGGTGGTGG+TGG + chr2_1:5873576-5873595 None:intergenic 55.0%
CCACCACCAAAAAGAGAGCC+GGG - chr2_1:5873576-5873595 Msa0190360:intron 55.0%
! AGCCGGGTTTTCTTGAGGGA+TGG - chr2_1:5873592-5873611 Msa0190360:intron 55.0%
CACAGCAGCACTCATCCAAG+AGG + chr2_1:5873773-5873792 None:intergenic 55.0%
CCGGAGGTGGTCCTTGATAA+GGG + chr2_1:5873521-5873540 None:intergenic 55.0%
CCCTTATCAAGGACCACCTC+CGG - chr2_1:5873518-5873537 Msa0190360:CDS 55.0%
ACCGGAGGTGGTCCTTGATA+AGG + chr2_1:5873522-5873541 None:intergenic 55.0%
TTATCAAGGACCACCTCCGG+TGG - chr2_1:5873521-5873540 Msa0190360:CDS 55.0%
GGAGCAGCATAGTATTGCGG+TGG + chr2_1:5873552-5873571 None:intergenic 55.0%
!!! GGCTCTCTTTTTGGTGGTGG+TGG + chr2_1:5873576-5873595 None:intergenic 55.0%
CCACCACCAAAAAGAGAGCC+GGG - chr2_1:5873576-5873595 Msa0190360:intron 55.0%
! AGCCGGGTTTTCTTGAGGGA+TGG - chr2_1:5873592-5873611 Msa0190360:intron 55.0%
CACAGCAGCACTCATCCAAG+AGG + chr2_1:5873773-5873792 None:intergenic 55.0%
!! CCCGGCTCTCTTTTTGGTGG+TGG + chr2_1:5873579-5873598 None:intergenic 60.0%
!! CCCGGCTCTCTTTTTGGTGG+TGG + chr2_1:5873579-5873598 None:intergenic 60.0%
TCAAGGACCACCTCCGGTGG+CGG - chr2_1:5873524-5873543 Msa0190360:CDS 65.0%
!! TATTGCGGTGGTGCCGCCAC+CGG + chr2_1:5873540-5873559 None:intergenic 65.0%
TCAAGGACCACCTCCGGTGG+CGG - chr2_1:5873524-5873543 Msa0190360:CDS 65.0%
!! TATTGCGGTGGTGCCGCCAC+CGG + chr2_1:5873540-5873559 None:intergenic 65.0%
!!! GGTGGTGCCGCCACCGGAGG+TGG + chr2_1:5873534-5873553 None:intergenic 80.0%
!!! TGCGGTGGTGCCGCCACCGG+AGG + chr2_1:5873537-5873556 None:intergenic 80.0%
!!! GGTGGTGCCGCCACCGGAGG+TGG + chr2_1:5873534-5873553 None:intergenic 80.0%
!!! TGCGGTGGTGCCGCCACCGG+AGG + chr2_1:5873537-5873556 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 5873178 5874137 5873178 ID=Msa0190360;Name=Msa0190360
chr2_1 mRNA 5873178 5874137 5873178 ID=Msa0190360-mRNA-1;Parent=Msa0190360;Name=Msa0190360-mRNA-1;_AED=0.00;_eAED=0.00;_QI=115|1|1|1|1|1|3|319|69
chr2_1 exon 5873178 5873569 5873178 ID=Msa0190360-mRNA-1:exon:2640;Parent=Msa0190360-mRNA-1
chr2_1 exon 5873702 5873798 5873702 ID=Msa0190360-mRNA-1:exon:2639;Parent=Msa0190360-mRNA-1
chr2_1 exon 5873983 5874137 5873983 ID=Msa0190360-mRNA-1:exon:2638;Parent=Msa0190360-mRNA-1
chr2_1 five_prime_UTR 5874023 5874137 5874023 ID=Msa0190360-mRNA-1:five_prime_utr;Parent=Msa0190360-mRNA-1
chr2_1 CDS 5873983 5874022 5873983 ID=Msa0190360-mRNA-1:cds;Parent=Msa0190360-mRNA-1
chr2_1 CDS 5873702 5873798 5873702 ID=Msa0190360-mRNA-1:cds;Parent=Msa0190360-mRNA-1
chr2_1 CDS 5873497 5873569 5873497 ID=Msa0190360-mRNA-1:cds;Parent=Msa0190360-mRNA-1
chr2_1 three_prime_UTR 5873178 5873496 5873178 ID=Msa0190360-mRNA-1:three_prime_utr;Parent=Msa0190360-mRNA-1
Gene Sequence

>Msa0190360

ATGAGTGAGATCAAATATGGCTATCCATATCCTGCTCAAGGCCCTTATCAAGGACCACCTCCGGTGGCGGCACCACCGCAATACTATGCTGCTCCACCACCACCACCAAAAAGAGAGCCGGGTTTTCTTGAGGGATGCCTAGCAGCTTTGTGTTGCTGTTGCCTCTTGGATGAGTGCTGCTGTGACCCAACTATTATTTTTGCGAGCTAA

Protein sequence

>Msa0190360

MSEIKYGYPYPAQGPYQGPPPVAAPPQYYAAPPPPPKREPGFLEGCLAALCCCCLLDECCCDPTIIFAS*