AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0316430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0316430 MtrunA17_Chr2g0288831 99.099 222 2 0 1 222 1 222 2.16e-160 442
Msa0316430 MtrunA17_Chr4g0024941 61.086 221 85 1 1 221 1 220 8.06e-97 281
Msa0316430 MtrunA17_Chr2g0322421 51.584 221 106 1 1 221 1 220 1.13e-68 210
Msa0316430 MtrunA17_Chr7g0235621 44.131 213 110 3 1 205 1 212 1.30e-49 161
Msa0316430 MtrunA17_Chr5g0444551 49.306 144 72 1 1 144 1 143 4.48e-44 144
Msa0316430 MtrunA17_Chr5g0444571 51.579 95 45 1 1 95 1 94 1.03e-28 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0316430 AT5G42570.1 64.593 209 73 1 1 209 1 208 4.32e-97 282
Msa0316430 AT1G11905.1 59.524 210 84 1 1 210 1 209 1.07e-84 251
Msa0316430 AT1G11905.2 55.714 210 91 2 1 210 1 208 4.39e-73 221
Msa0316430 AT5G48660.1 42.056 214 115 3 1 206 1 213 2.04e-44 148
Msa0316430 AT3G07190.1 41.121 214 117 3 1 206 1 213 3.04e-42 142
Msa0316430 AT3G20450.1 50.376 133 65 1 1 132 5 137 1.08e-37 128
Msa0316430 AT5G48660.2 37.226 137 79 1 77 206 1 137 4.29e-19 80.9

Find 72 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTTTGTTAGTACAAAAT+TGG 0.147691 2_4:-13567958 None:intergenic
CATCACACACACAGAGTTTA+TGG 0.163535 2_4:-13565116 None:intergenic
TGTTTCTCACATATTCTTCT+TGG 0.219887 2_4:-13567808 None:intergenic
TCCTACCGATCAGGTTCTTA+TGG 0.252341 2_4:+13565450 Msa0316430:CDS
CATAGCAGCTTGCTAGTAAC+AGG 0.279190 2_4:+13567871 Msa0316430:three_prime_UTR
AAACATGCTTCTCATGAAAT+AGG 0.301312 2_4:+13567908 Msa0316430:three_prime_UTR
GTAGCTGGTTGCGGAGGTTT+TGG 0.307160 2_4:-13567752 None:intergenic
TACACCGATCAAGAGTGTTT+TGG 0.308796 2_4:-13565184 None:intergenic
TTCATGAGAAGCATGTTTAC+AGG 0.318501 2_4:-13567903 None:intergenic
ATTGAGGAAGCTTGTAATAA+TGG 0.322813 2_4:+13565288 Msa0316430:CDS
CTATCATAAGTGCAAGAAAA+AGG 0.327998 2_4:-13567449 None:intergenic
GAATATGTGAGAAACATTTA+AGG 0.355673 2_4:+13567815 Msa0316430:exon
GAAGCTTGTAATAATGGCTT+TGG 0.364769 2_4:+13565294 Msa0316430:CDS
CCATGCTCTTTCTCCGAATC+CGG 0.367578 2_4:-13567500 None:intergenic
CACTTTGTTAGTACAAAATT+GGG 0.372520 2_4:-13567957 None:intergenic
TTTAGTTTCATCTGAAGTTC+CGG 0.385457 2_4:-13567565 None:intergenic
TTATTACAAGCTTCCTCAAT+GGG 0.391866 2_4:-13565285 None:intergenic
AAATAGTGCTTGATATCTCT+TGG 0.410580 2_4:+13568093 Msa0316430:three_prime_UTR
AAGCCAGTGTAAGTGCTCTA+AGG 0.421670 2_4:+13567679 Msa0316430:CDS
ATGGGGTGAGTGAGAGGTTG+TGG 0.423024 2_4:-13565097 None:intergenic
CTTCTGTTCCTTCATTATGA+AGG 0.425413 2_4:+13568059 Msa0316430:three_prime_UTR
GGGGCTGAGGATGCGAAAGC+CGG 0.426768 2_4:+13567546 Msa0316430:CDS
TTCTGTTAATCCTACCGATC+AGG 0.442382 2_4:+13565441 Msa0316430:CDS
ATCACACACACAGAGTTTAT+GGG 0.442629 2_4:-13565115 None:intergenic
ACAGACTGCATCATTACATA+AGG 0.453424 2_4:+13567472 Msa0316430:CDS
TATTATCATTGTTTCTTCTG+TGG 0.458311 2_4:+13568136 Msa0316430:three_prime_UTR
ACATAAGGGAACTCCGGATT+CGG 0.458900 2_4:+13567487 Msa0316430:CDS
ACGAGGTCGTGGACCTGTTA+TGG 0.464283 2_4:+13565327 Msa0316430:CDS
TTCTGTCAACAGATTGTAGC+TGG 0.470135 2_4:-13567767 None:intergenic
ATTATTACAAGCTTCCTCAA+TGG 0.471169 2_4:-13565286 None:intergenic
CTGTTACTAGCAAGCTGCTA+TGG 0.480052 2_4:-13567870 None:intergenic
GAGAGCAGAAGTTGATCGTC+TGG 0.490422 2_4:+13567614 Msa0316430:CDS
AGATATCAAGCACTATTTCA+AGG 0.505263 2_4:-13568088 None:intergenic
CAACAGATTGTAGCTGGTTG+CGG 0.507849 2_4:-13567761 None:intergenic
GTTATGGTGAAAACCGTTGC+TGG 0.514910 2_4:+13565343 Msa0316430:CDS
GGCACATTCCTTCATAATGA+AGG 0.518873 2_4:-13568067 None:intergenic
TTCAGATGAAACTAAAACAA+TGG 0.522908 2_4:+13567572 Msa0316430:CDS
GATCGGTTGAAACGAGGTCG+TGG 0.523142 2_4:+13565316 Msa0316430:CDS
ACTTCAGTCAAAAGATAAAG+AGG 0.526615 2_4:+13567644 Msa0316430:CDS
TCATGAGAAGCATGTTTACA+GGG 0.527012 2_4:-13567902 None:intergenic
GTCAATATAATAGAACTCTC+TGG 0.527974 2_4:-13568173 None:intergenic
CAACAACGTGGGATCGAAGA+TGG 0.533524 2_4:+13565415 Msa0316430:CDS
TGAATATGATCGCTTACTTG+AGG 0.542275 2_4:+13567725 Msa0316430:CDS
TTGTAATAATGGCTTTGGAT+CGG 0.542465 2_4:+13565299 Msa0316430:CDS
AGCTGTTAAGAAACAAATGC+GGG 0.550293 2_4:+13567524 Msa0316430:CDS
TCTTCTTGAAGCTACACTCA+TGG 0.550488 2_4:+13565480 Msa0316430:CDS
GAGTTTATGGGGTGAGTGAG+AGG 0.552067 2_4:-13565103 None:intergenic
CAGACTGCATCATTACATAA+GGG 0.555282 2_4:+13567473 Msa0316430:CDS
CTTCTTGAAGCTACACTCAT+GGG 0.557141 2_4:+13565481 Msa0316430:CDS
CAGATTGTAGCTGGTTGCGG+AGG 0.567147 2_4:-13567758 None:intergenic
GCTCTAAGGAAACAATCTGA+AGG 0.569864 2_4:+13567693 Msa0316430:CDS
ATCATTACATAAGGGAACTC+CGG 0.575747 2_4:+13567481 Msa0316430:CDS
GCCATAAGAACCTGATCGGT+AGG 0.586898 2_4:-13565451 None:intergenic
CTCTAAGGAAACAATCTGAA+GGG 0.587548 2_4:+13567694 Msa0316430:CDS
CAGTCAAAAGATAAAGAGGT+AGG 0.596716 2_4:+13567648 Msa0316430:CDS
CCGGATTCGGAGAAAGAGCA+TGG 0.597596 2_4:+13567500 Msa0316430:CDS
GCTTTGGATCGGTTGAAACG+AGG 0.607033 2_4:+13565310 Msa0316430:CDS
AGATCCAAAACACTCTTGAT+CGG 0.608818 2_4:+13565180 Msa0316430:five_prime_UTR
TTTCCTTAGAGCACTTACAC+TGG 0.609208 2_4:-13567682 None:intergenic
CTTAGCCATAAGAACCTGAT+CGG 0.613122 2_4:-13565455 None:intergenic
AACAAGAACAGTACCAGCAA+CGG 0.621936 2_4:-13565356 None:intergenic
TCACACACACAGAGTTTATG+GGG 0.626002 2_4:-13565114 None:intergenic
CTGTTAAGAAACAAATGCGG+GGG 0.642686 2_4:+13567526 Msa0316430:CDS
TGTTATTCAAGACCCCATTG+AGG 0.652558 2_4:+13565272 Msa0316430:CDS
GAATTGCTTCATCACCGACA+AGG 0.655249 2_4:+13565155 Msa0316430:five_prime_UTR
TGATGAAGATTCAACAACGT+GGG 0.655476 2_4:+13565404 Msa0316430:CDS
TATGTGAGAAACATTTAAGG+CGG 0.655679 2_4:+13567818 Msa0316430:exon
GCTGTTAAGAAACAAATGCG+GGG 0.673517 2_4:+13567525 Msa0316430:CDS
TATTACAAGCTTCCTCAATG+GGG 0.687342 2_4:-13565284 None:intergenic
AAGCTGTTAAGAAACAAATG+CGG 0.709469 2_4:+13567523 Msa0316430:CDS
TGTTAAGAAACAAATGCGGG+GGG 0.715839 2_4:+13567527 Msa0316430:CDS
ATGATGAAGATTCAACAACG+TGG 0.740230 2_4:+13565403 Msa0316430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCATAATATGAAATAAAA+GGG - chr2_4:13566416-13566435 None:intergenic 10.0%
!! TATTCATAATATGAAATAAA+AGG - chr2_4:13566417-13566436 None:intergenic 10.0%
!! TATATTATTAAACTTATTAG+TGG + chr2_4:13566497-13566516 Msa0316430:intron 10.0%
!! TTTAATAAGTCTAAATATTA+AGG - chr2_4:13567369-13567388 None:intergenic 10.0%
!!! TGTGAATTTTTTTTTTTTTT+TGG + chr2_4:13567978-13567997 Msa0316430:three_prime_UTR 10.0%
!!! TTGAATTTGATTTATGTTTA+TGG + chr2_4:13565582-13565601 Msa0316430:intron 15.0%
!!! TGAATTTGATTTATGTTTAT+GGG + chr2_4:13565583-13565602 Msa0316430:intron 15.0%
!!! TTTTGTAAATATGATCAAAT+AGG + chr2_4:13565681-13565700 Msa0316430:intron 15.0%
!!! TTTTACTAGTTGTATATAAT+TGG + chr2_4:13566365-13566384 Msa0316430:intron 15.0%
!!! AGTATTTCTTTTAAATAGTA+TGG + chr2_4:13566472-13566491 Msa0316430:intron 15.0%
!!! GTTATTGTTTTACTATTGAT+AGG - chr2_4:13566345-13566364 None:intergenic 20.0%
!! AATCATAAGATATATGCATA+TGG - chr2_4:13566735-13566754 None:intergenic 20.0%
!! TTCTAAACCATTGAAAAAAT+CGG - chr2_4:13566957-13566976 None:intergenic 20.0%
!! ATTGTTTATTGATGAGTTAT+TGG + chr2_4:13567063-13567082 Msa0316430:intron 20.0%
!!! CTATATGATTTGCAATTTTT+AGG + chr2_4:13567118-13567137 Msa0316430:intron 20.0%
!!! TTTTACTTCAGAGTTAAATT+TGG + chr2_4:13567933-13567952 Msa0316430:three_prime_UTR 20.0%
!! GAATGAATGAATTTTGTATG+AGG + chr2_4:13565557-13565576 Msa0316430:intron 25.0%
! TTGATTTATGTTTATGGGTT+TGG + chr2_4:13565588-13565607 Msa0316430:intron 25.0%
!! ATTTTGCTTATAACTAAGAC+TGG + chr2_4:13565725-13565744 Msa0316430:intron 25.0%
! CTACATTAATGAAAGGAAAA+GGG - chr2_4:13565997-13566016 None:intergenic 25.0%
! ACTACATTAATGAAAGGAAA+AGG - chr2_4:13565998-13566017 None:intergenic 25.0%
!! ACATTGACTACATTAATGAA+AGG - chr2_4:13566004-13566023 None:intergenic 25.0%
!!! GAAGTTTTGAGTAATGTTTA+AGG + chr2_4:13566443-13566462 Msa0316430:intron 25.0%
!! AATCATTGTGATTCTTTTTC+AGG - chr2_4:13566681-13566700 None:intergenic 25.0%
! AAGTTTAACAACATACTAGA+TGG - chr2_4:13567167-13567186 None:intergenic 25.0%
! TTTGGAAATATGTTGAACTT+AGG - chr2_4:13567214-13567233 None:intergenic 25.0%
!!! CAGCAATGTTTTGAAAAATT+TGG - chr2_4:13567232-13567251 None:intergenic 25.0%
!!! TCTTTGATTTTTAGTAAGAC+CGG + chr2_4:13567255-13567274 Msa0316430:intron 25.0%
! TTATTAAAAGATATCCTCTG+TGG + chr2_4:13567381-13567400 Msa0316430:intron 25.0%
! TTCAGATGAAACTAAAACAA+TGG + chr2_4:13567572-13567591 Msa0316430:CDS 25.0%
! GAATATGTGAGAAACATTTA+AGG + chr2_4:13567815-13567834 Msa0316430:exon 25.0%
!! TTCTTCTCTTCTATACTTTT+TGG + chr2_4:13567842-13567861 Msa0316430:three_prime_UTR 25.0%
!!! TCTTCTCTTCTATACTTTTT+GGG + chr2_4:13567843-13567862 Msa0316430:three_prime_UTR 25.0%
! CACTTTGTTAGTACAAAATT+GGG - chr2_4:13567960-13567979 None:intergenic 25.0%
! ACACTTTGTTAGTACAAAAT+TGG - chr2_4:13567961-13567980 None:intergenic 25.0%
!! TATTATCATTGTTTCTTCTG+TGG + chr2_4:13568136-13568155 Msa0316430:three_prime_UTR 25.0%
TTATTACAAGCTTCCTCAAT+GGG - chr2_4:13565288-13565307 None:intergenic 30.0%
ATTATTACAAGCTTCCTCAA+TGG - chr2_4:13565289-13565308 None:intergenic 30.0%
ATTGAGGAAGCTTGTAATAA+TGG + chr2_4:13565288-13565307 Msa0316430:CDS 30.0%
!! TTGTAATAATGGCTTTGGAT+CGG + chr2_4:13565299-13565318 Msa0316430:CDS 30.0%
! ACATTTGTATGAGTGTTGTA+CGG + chr2_4:13565846-13565865 Msa0316430:intron 30.0%
! TGATCAATCACAAAACCTTT+TGG + chr2_4:13565912-13565931 Msa0316430:intron 30.0%
AGATCTTCTCAATTTCCAAA+AGG - chr2_4:13565930-13565949 None:intergenic 30.0%
! GAACCCATTTTCTAATCTTT+CGG + chr2_4:13565962-13565981 Msa0316430:intron 30.0%
ACTCCGAAAGATTAGAAAAT+GGG - chr2_4:13565968-13565987 None:intergenic 30.0%
!!! GATTCTTTTTCAGGAAAACA+CGG - chr2_4:13566672-13566691 None:intergenic 30.0%
ACAATGATTCAGCATTCATA+TGG + chr2_4:13566692-13566711 Msa0316430:intron 30.0%
TTGTTTGCCTAATTCTTTGT+TGG + chr2_4:13566897-13566916 Msa0316430:intron 30.0%
TGTTTGCCTAATTCTTTGTT+GGG + chr2_4:13566898-13566917 Msa0316430:intron 30.0%
GAATAACCCAACAAAGAATT+AGG - chr2_4:13566907-13566926 None:intergenic 30.0%
!!! TTTGCTACCGATTTTTTCAA+TGG + chr2_4:13566947-13566966 Msa0316430:intron 30.0%
! ATGGTTTAGAATGAATGTTG+AGG + chr2_4:13566966-13566985 Msa0316430:intron 30.0%
AAGATGAAAAATGTGAGCTT+TGG + chr2_4:13567017-13567036 Msa0316430:intron 30.0%
GTTTATTGATGAGTTATTGG+TGG + chr2_4:13567066-13567085 Msa0316430:intron 30.0%
!!! ATGTTGTTTTAAGATGTGAC+CGG - chr2_4:13567277-13567296 None:intergenic 30.0%
TTAAAACAACATAGCTGAAG+AGG + chr2_4:13567285-13567304 Msa0316430:intron 30.0%
CAGTATTGTTTATGTTGGAT+TGG - chr2_4:13567324-13567343 None:intergenic 30.0%
CTCAACAGTATTGTTTATGT+TGG - chr2_4:13567329-13567348 None:intergenic 30.0%
ATTCTTGGTCAATTATTTGC+AGG + chr2_4:13567420-13567439 Msa0316430:intron 30.0%
CTATCATAAGTGCAAGAAAA+AGG - chr2_4:13567452-13567471 None:intergenic 30.0%
AAGCTGTTAAGAAACAAATG+CGG + chr2_4:13567523-13567542 Msa0316430:CDS 30.0%
! TTTAGTTTCATCTGAAGTTC+CGG - chr2_4:13567568-13567587 None:intergenic 30.0%
ACTTCAGTCAAAAGATAAAG+AGG + chr2_4:13567644-13567663 Msa0316430:CDS 30.0%
TGTTTCTCACATATTCTTCT+TGG - chr2_4:13567811-13567830 None:intergenic 30.0%
TATGTGAGAAACATTTAAGG+CGG + chr2_4:13567818-13567837 Msa0316430:exon 30.0%
AAACATGCTTCTCATGAAAT+AGG + chr2_4:13567908-13567927 Msa0316430:three_prime_UTR 30.0%
AGATATCAAGCACTATTTCA+AGG - chr2_4:13568091-13568110 None:intergenic 30.0%
AAATAGTGCTTGATATCTCT+TGG + chr2_4:13568093-13568112 Msa0316430:three_prime_UTR 30.0%
ATCACACACACAGAGTTTAT+GGG - chr2_4:13565118-13565137 None:intergenic 35.0%
!! TGTTTTGGATCTTACCTTGT+CGG - chr2_4:13565172-13565191 None:intergenic 35.0%
AGATCCAAAACACTCTTGAT+CGG + chr2_4:13565180-13565199 Msa0316430:five_prime_UTR 35.0%
TATTACAAGCTTCCTCAATG+GGG - chr2_4:13565287-13565306 None:intergenic 35.0%
GAAGCTTGTAATAATGGCTT+TGG + chr2_4:13565294-13565313 Msa0316430:CDS 35.0%
ATGATGAAGATTCAACAACG+TGG + chr2_4:13565403-13565422 Msa0316430:CDS 35.0%
TGATGAAGATTCAACAACGT+GGG + chr2_4:13565404-13565423 Msa0316430:CDS 35.0%
! CTCATGGGTATGTTTTCTTA+TGG + chr2_4:13565496-13565515 Msa0316430:intron 35.0%
TGCTTATAACTAAGACTGGA+CGG + chr2_4:13565729-13565748 Msa0316430:intron 35.0%
! GTCATATCTAGTTTCCGTAT+CGG - chr2_4:13565776-13565795 None:intergenic 35.0%
! AGAACGTTTTGTATGAGTTG+TGG + chr2_4:13565820-13565839 Msa0316430:intron 35.0%
! GAACGTTTTGTATGAGTTGT+GGG + chr2_4:13565821-13565840 Msa0316430:intron 35.0%
GACTCCGAAAGATTAGAAAA+TGG - chr2_4:13565969-13565988 None:intergenic 35.0%
AAGAACCATTCGAGAATTGA+AGG - chr2_4:13566138-13566157 None:intergenic 35.0%
TTCAGCATTCATATGGCATA+AGG + chr2_4:13566699-13566718 Msa0316430:intron 35.0%
!!! TTGGTATTTTAGCCTCCTAA+TGG + chr2_4:13566804-13566823 Msa0316430:intron 35.0%
!!! TGGTATTTTAGCCTCCTAAT+GGG + chr2_4:13566805-13566824 Msa0316430:intron 35.0%
GAGAATGTTCTACTTTCTTG+TGG + chr2_4:13567039-13567058 Msa0316430:intron 35.0%
TTGCGAGCATTATTCATTCT+TGG + chr2_4:13567405-13567424 Msa0316430:intron 35.0%
ACAGACTGCATCATTACATA+AGG + chr2_4:13567472-13567491 Msa0316430:CDS 35.0%
CAGACTGCATCATTACATAA+GGG + chr2_4:13567473-13567492 Msa0316430:CDS 35.0%
ATCATTACATAAGGGAACTC+CGG + chr2_4:13567481-13567500 Msa0316430:CDS 35.0%
AGCTGTTAAGAAACAAATGC+GGG + chr2_4:13567524-13567543 Msa0316430:CDS 35.0%
CAGTCAAAAGATAAAGAGGT+AGG + chr2_4:13567648-13567667 Msa0316430:CDS 35.0%
! CTCTAAGGAAACAATCTGAA+GGG + chr2_4:13567694-13567713 Msa0316430:CDS 35.0%
TGAATATGATCGCTTACTTG+AGG + chr2_4:13567725-13567744 Msa0316430:CDS 35.0%
! TCATGAGAAGCATGTTTACA+GGG - chr2_4:13567905-13567924 None:intergenic 35.0%
! TTCATGAGAAGCATGTTTAC+AGG - chr2_4:13567906-13567925 None:intergenic 35.0%
CTTCTGTTCCTTCATTATGA+AGG + chr2_4:13568059-13568078 Msa0316430:three_prime_UTR 35.0%
TCACACACACAGAGTTTATG+GGG - chr2_4:13565117-13565136 None:intergenic 40.0%
CATCACACACACAGAGTTTA+TGG - chr2_4:13565119-13565138 None:intergenic 40.0%
!!! TACACCGATCAAGAGTGTTT+TGG - chr2_4:13565187-13565206 None:intergenic 40.0%
TGTTATTCAAGACCCCATTG+AGG + chr2_4:13565272-13565291 Msa0316430:CDS 40.0%
AACAAGAACAGTACCAGCAA+CGG - chr2_4:13565359-13565378 None:intergenic 40.0%
TTCTGTTAATCCTACCGATC+AGG + chr2_4:13565441-13565460 Msa0316430:CDS 40.0%
CTTAGCCATAAGAACCTGAT+CGG - chr2_4:13565458-13565477 None:intergenic 40.0%
TCTTCTTGAAGCTACACTCA+TGG + chr2_4:13565480-13565499 Msa0316430:CDS 40.0%
CTTCTTGAAGCTACACTCAT+GGG + chr2_4:13565481-13565500 Msa0316430:CDS 40.0%
! TTTTGACAACAGAGGACAAG+AGG - chr2_4:13566222-13566241 None:intergenic 40.0%
TTGTCCTCTGTTGTCAAAAG+CGG + chr2_4:13566223-13566242 Msa0316430:intron 40.0%
CTGTTGTCAATGCGGAAATA+CGG - chr2_4:13566558-13566577 None:intergenic 40.0%
AATACACGTTCACGAGGTAT+AGG - chr2_4:13566644-13566663 None:intergenic 40.0%
! GTTTGAAATACACGTTCACG+AGG - chr2_4:13566650-13566669 None:intergenic 40.0%
GCTAAAATACCAAGAGTGAC+CGG - chr2_4:13566797-13566816 None:intergenic 40.0%
AATCATATGAGCCCATTAGG+AGG - chr2_4:13566819-13566838 None:intergenic 40.0%
CTGAATCATATGAGCCCATT+AGG - chr2_4:13566822-13566841 None:intergenic 40.0%
CTTGTAACTTAAAGCCTCGT+TGG + chr2_4:13566874-13566893 Msa0316430:intron 40.0%
AATTAGGCAAACAACCAACG+AGG - chr2_4:13566891-13566910 None:intergenic 40.0%
! AGTTATTGGTGGCATTAGCA+GGG + chr2_4:13567077-13567096 Msa0316430:intron 40.0%
GCTGTTAAGAAACAAATGCG+GGG + chr2_4:13567525-13567544 Msa0316430:CDS 40.0%
CTGTTAAGAAACAAATGCGG+GGG + chr2_4:13567526-13567545 Msa0316430:CDS 40.0%
TGTTAAGAAACAAATGCGGG+GGG + chr2_4:13567527-13567546 Msa0316430:CDS 40.0%
! TTTCCTTAGAGCACTTACAC+TGG - chr2_4:13567685-13567704 None:intergenic 40.0%
!! GCTCTAAGGAAACAATCTGA+AGG + chr2_4:13567693-13567712 Msa0316430:CDS 40.0%
TTCTGTCAACAGATTGTAGC+TGG - chr2_4:13567770-13567789 None:intergenic 40.0%
GGCACATTCCTTCATAATGA+AGG - chr2_4:13568070-13568089 None:intergenic 40.0%
GAATTGCTTCATCACCGACA+AGG + chr2_4:13565155-13565174 Msa0316430:five_prime_UTR 45.0%
! GCAACGGTTTTCACCATAAC+AGG - chr2_4:13565343-13565362 None:intergenic 45.0%
GTTATGGTGAAAACCGTTGC+TGG + chr2_4:13565343-13565362 Msa0316430:CDS 45.0%
TCCTACCGATCAGGTTCTTA+TGG + chr2_4:13565450-13565469 Msa0316430:CDS 45.0%
! TCTAGTTTCCGTATCGGATC+CGG - chr2_4:13565770-13565789 None:intergenic 45.0%
GTTGTACGGATGTCATACAC+CGG + chr2_4:13565860-13565879 Msa0316430:intron 45.0%
TCTTGAGTTTACTGTGCCTC+CGG + chr2_4:13566154-13566173 Msa0316430:intron 45.0%
CGGAGCATAGCAGAAAAATC+AGG + chr2_4:13566264-13566283 Msa0316430:intron 45.0%
TCTAGAAGCTGAACCTAGAC+AGG - chr2_4:13566585-13566604 None:intergenic 45.0%
! GAGTTATTGGTGGCATTAGC+AGG + chr2_4:13567076-13567095 Msa0316430:intron 45.0%
ACATAAGGGAACTCCGGATT+CGG + chr2_4:13567487-13567506 Msa0316430:CDS 45.0%
AAGCCAGTGTAAGTGCTCTA+AGG + chr2_4:13567679-13567698 Msa0316430:CDS 45.0%
CAACAGATTGTAGCTGGTTG+CGG - chr2_4:13567764-13567783 None:intergenic 45.0%
CTGTTACTAGCAAGCTGCTA+TGG - chr2_4:13567873-13567892 None:intergenic 45.0%
!! CATAGCAGCTTGCTAGTAAC+AGG + chr2_4:13567871-13567890 Msa0316430:three_prime_UTR 45.0%
GAGTTTATGGGGTGAGTGAG+AGG - chr2_4:13565106-13565125 None:intergenic 50.0%
! GCTTTGGATCGGTTGAAACG+AGG + chr2_4:13565310-13565329 Msa0316430:CDS 50.0%
CAACAACGTGGGATCGAAGA+TGG + chr2_4:13565415-13565434 Msa0316430:CDS 50.0%
GCCATAAGAACCTGATCGGT+AGG - chr2_4:13565454-13565473 None:intergenic 50.0%
! ACGGAGTATCTGTCAAGCAC+CGG + chr2_4:13565748-13565767 Msa0316430:intron 50.0%
TGACACTGACACAGACACGT+TGG + chr2_4:13565792-13565811 Msa0316430:intron 50.0%
TGTCATACACCGGTGTGTGT+TGG + chr2_4:13565870-13565889 Msa0316430:intron 50.0%
TCTCTGTGACCAACACACAC+CGG - chr2_4:13565882-13565901 None:intergenic 50.0%
TTGCTGCATCTGATGCAGCA+TGG + chr2_4:13566109-13566128 Msa0316430:intron 50.0%
GGCTGCCTTCAATTCTCGAA+TGG + chr2_4:13566130-13566149 Msa0316430:intron 50.0%
TTTGACAGGGCAAAGCCTCT+TGG - chr2_4:13566193-13566212 None:intergenic 50.0%
AGAGGCTTTGCCCTGTCAAA+CGG + chr2_4:13566193-13566212 Msa0316430:intron 50.0%
CAAGAGGAGTCCGTTTGACA+GGG - chr2_4:13566206-13566225 None:intergenic 50.0%
ACAAGAGGAGTCCGTTTGAC+AGG - chr2_4:13566207-13566226 None:intergenic 50.0%
TCTGCTATGCTCCGCTATAG+CGG - chr2_4:13566258-13566277 None:intergenic 50.0%
CGGGTATCAGAAGTGGAAAG+CGG + chr2_4:13566310-13566329 Msa0316430:intron 50.0%
AGAAGTGGAAAGCGGCAAAG+CGG + chr2_4:13566318-13566337 Msa0316430:intron 50.0%
AAATACGGTGTGGCGCATAG+CGG - chr2_4:13566543-13566562 None:intergenic 50.0%
GTCAATGCGGAAATACGGTG+TGG - chr2_4:13566553-13566572 None:intergenic 50.0%
CAGGAGCTCTGTTGTCAATG+CGG - chr2_4:13566566-13566585 None:intergenic 50.0%
TGACAACAGAGCTCCTGTCT+AGG + chr2_4:13566569-13566588 Msa0316430:intron 50.0%
AGAGAAATAGCCTTCGCCCT+TGG + chr2_4:13566766-13566785 Msa0316430:intron 50.0%
GAGAAATAGCCTTCGCCCTT+GGG + chr2_4:13566767-13566786 Msa0316430:intron 50.0%
! AGAGTGACCGGTTTACCCAA+GGG - chr2_4:13566785-13566804 None:intergenic 50.0%
! AAGAGTGACCGGTTTACCCA+AGG - chr2_4:13566786-13566805 None:intergenic 50.0%
TTGGGTAAACCGGTCACTCT+TGG + chr2_4:13566785-13566804 Msa0316430:intron 50.0%
ATAATGCTCGCAAGCCACAG+AGG - chr2_4:13567398-13567417 None:intergenic 50.0%
CCATGCTCTTTCTCCGAATC+CGG - chr2_4:13567503-13567522 None:intergenic 50.0%
! GAGAGCAGAAGTTGATCGTC+TGG + chr2_4:13567614-13567633 Msa0316430:CDS 50.0%
GATCGGTTGAAACGAGGTCG+TGG + chr2_4:13565316-13565335 Msa0316430:CDS 55.0%
ACGAGGTCGTGGACCTGTTA+TGG + chr2_4:13565327-13565346 Msa0316430:CDS 55.0%
!! GTCAAGCACCGGATCCGATA+CGG + chr2_4:13565759-13565778 Msa0316430:intron 55.0%
! TCTTGGACTGGGGAAAGAGC+CGG - chr2_4:13566176-13566195 None:intergenic 55.0%
AGGGCAAAGCCTCTTGGACT+GGG - chr2_4:13566187-13566206 None:intergenic 55.0%
!!! GTGGCCGCTTTTGACAACAG+AGG - chr2_4:13566230-13566249 None:intergenic 55.0%
GTTGTCAAAAGCGGCCACAC+CGG + chr2_4:13566232-13566251 Msa0316430:intron 55.0%
AAAATCAGGACCACTGCGCC+AGG + chr2_4:13566278-13566297 Msa0316430:intron 55.0%
AATACGGTGTGGCGCATAGC+GGG - chr2_4:13566542-13566561 None:intergenic 55.0%
!! ACTGGAAGAGGTGCCAACAG+GGG - chr2_4:13566620-13566639 None:intergenic 55.0%
!! CACTGGAAGAGGTGCCAACA+GGG - chr2_4:13566621-13566640 None:intergenic 55.0%
!! CGAGGTATAGGCACTGGAAG+AGG - chr2_4:13566632-13566651 None:intergenic 55.0%
CGTTCACGAGGTATAGGCAC+TGG - chr2_4:13566638-13566657 None:intergenic 55.0%
CCGGATTCGGAGAAAGAGCA+TGG + chr2_4:13567500-13567519 Msa0316430:CDS 55.0%
! GTAGCTGGTTGCGGAGGTTT+TGG - chr2_4:13567755-13567774 None:intergenic 55.0%
CAGATTGTAGCTGGTTGCGG+AGG - chr2_4:13567761-13567780 None:intergenic 55.0%
GGCTCTTTCCCCAGTCCAAG+AGG + chr2_4:13566175-13566194 Msa0316430:intron 60.0%
GGGCAAAGCCTCTTGGACTG+GGG - chr2_4:13566186-13566205 None:intergenic 60.0%
CAGGGCAAAGCCTCTTGGAC+TGG - chr2_4:13566188-13566207 None:intergenic 60.0%
CTATGCTCCGCTATAGCGGC+CGG - chr2_4:13566254-13566273 None:intergenic 60.0%
ACTTCTGATACCCGCCGACG+CGG - chr2_4:13566304-13566323 None:intergenic 60.0%
!! CATAGCGGGCGCTATTTCGG+TGG - chr2_4:13566528-13566547 None:intergenic 60.0%
!! GCGCATAGCGGGCGCTATTT+CGG - chr2_4:13566531-13566550 None:intergenic 60.0%
ACCTCTCTCAGCTCCCCTGT+TGG + chr2_4:13566604-13566623 Msa0316430:intron 60.0%
!! GCACTGGAAGAGGTGCCAAC+AGG - chr2_4:13566622-13566641 None:intergenic 60.0%
GCCTTCGCCCTTGGGTAAAC+CGG + chr2_4:13566775-13566794 Msa0316430:intron 60.0%
! ACCGGTTTACCCAAGGGCGA+AGG - chr2_4:13566779-13566798 None:intergenic 60.0%
GAAACAAATGCGGGGGGCTG+AGG + chr2_4:13567533-13567552 Msa0316430:CDS 60.0%
! TGGACTGGGGAAAGAGCCGG+AGG - chr2_4:13566173-13566192 None:intergenic 65.0%
! GCGTCGGCGGGTATCAGAAG+TGG + chr2_4:13566303-13566322 Msa0316430:intron 65.0%
GCCAACAGGGGAGCTGAGAG+AGG - chr2_4:13566608-13566627 None:intergenic 65.0%
GGGGCTGAGGATGCGAAAGC+CGG + chr2_4:13567546-13567565 Msa0316430:CDS 65.0%
GGCCACACCGGCCGCTATAG+CGG + chr2_4:13566244-13566263 Msa0316430:intron 70.0%
CTCCGCTATAGCGGCCGGTG+TGG - chr2_4:13566249-13566268 None:intergenic 70.0%
ACCACTGCGCCAGGCCGCGT+CGG + chr2_4:13566287-13566306 Msa0316430:intron 75.0%
TGATACCCGCCGACGCGGCC+TGG - chr2_4:13566299-13566318 None:intergenic 75.0%
! ACTGCGCCAGGCCGCGTCGG+CGG + chr2_4:13566290-13566309 Msa0316430:intron 80.0%
! GCCGACGCGGCCTGGCGCAG+TGG - chr2_4:13566291-13566310 None:intergenic 85.0%
!! CTGCGCCAGGCCGCGTCGGC+GGG + chr2_4:13566291-13566310 Msa0316430:intron 85.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 13565098 13568185 13565098 ID=Msa0316430;Name=Msa0316430
chr2_4 mRNA 13565098 13568185 13565098 ID=Msa0316430-mRNA-1;Parent=Msa0316430;Name=Msa0316430-mRNA-1;_AED=0.10;_eAED=0.10;_QI=119|1|1|1|1|1|2|361|222
chr2_4 exon 13565098 13565502 13565098 ID=Msa0316430-mRNA-1:exon:5091;Parent=Msa0316430-mRNA-1
chr2_4 exon 13567442 13568185 13567442 ID=Msa0316430-mRNA-1:exon:5092;Parent=Msa0316430-mRNA-1
chr2_4 five_prime_UTR 13565098 13565216 13565098 ID=Msa0316430-mRNA-1:five_prime_utr;Parent=Msa0316430-mRNA-1
chr2_4 CDS 13565217 13565502 13565217 ID=Msa0316430-mRNA-1:cds;Parent=Msa0316430-mRNA-1
chr2_4 CDS 13567442 13567824 13567442 ID=Msa0316430-mRNA-1:cds;Parent=Msa0316430-mRNA-1
chr2_4 three_prime_UTR 13567825 13568185 13567825 ID=Msa0316430-mRNA-1:three_prime_utr;Parent=Msa0316430-mRNA-1
Gene Sequence

>Msa0316430

ATGATTCATCTATTATTCACAGTGATTTTTTCAGAAGCAACAATGATCGCATTGCTGTTATTCAAGACCCCATTGAGGAAGCTTGTAATAATGGCTTTGGATCGGTTGAAACGAGGTCGTGGACCTGTTATGGTGAAAACCGTTGCTGGTACTGTTCTTGTTGTTTTGTCTTCTAGTGTTTATAGTATGATGAAGATTCAACAACGTGGGATCGAAGATGGTGCTTCTGTTAATCCTACCGATCAGGTTCTTATGGCTAAGCATCTTCTTGAAGCTACACTCATGGGTGCTATCCTTTTTCTTGCACTTATGATAGACAGACTGCATCATTACATAAGGGAACTCCGGATTCGGAGAAAGAGCATGGAAGCTGTTAAGAAACAAATGCGGGGGGCTGAGGATGCGAAAGCCGGAACTTCAGATGAAACTAAAACAATGGAGCAAGAAGCAGCTAAGTTGAGAGCAGAAGTTGATCGTCTGGAATCTGAACTTCAGTCAAAAGATAAAGAGGTAGGTGCTGCAGAAGCCAGTGTAAGTGCTCTAAGGAAACAATCTGAAGGGTTTCTTCTTGAATATGATCGCTTACTTGAGGAGAACCAAAACCTCCGCAACCAGCTACAATCTGTTGACAGAAGATTCTCACATTCAGTTTCCAAGAAGAATATGTGA
Protein sequence

>Msa0316430

MIHLLFTVIFSEATMIALLLFKTPLRKLVIMALDRLKRGRGPVMVKTVAGTVLVVLSSSVYSMMKIQQRGIEDGASVNPTDQVLMAKHLLEATLMGAILFLALMIDRLHHYIRELRIRRKSMEAVKKQMRGAEDAKAGTSDETKTMEQEAAKLRAEVDRLESELQSKDKEVGAAEASVSALRKQSEGFLLEYDRLLEENQNLRNQLQSVDRRFSHSVSKKNM*