AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0276380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0276380 MtrunA17_Chr2g0288831 99.099 222 2 0 1 222 1 222 2.16e-160 442
Msa0276380 MtrunA17_Chr4g0024941 61.086 221 85 1 1 221 1 220 8.06e-97 281
Msa0276380 MtrunA17_Chr2g0322421 51.584 221 106 1 1 221 1 220 1.13e-68 210
Msa0276380 MtrunA17_Chr7g0235621 44.131 213 110 3 1 205 1 212 1.30e-49 161
Msa0276380 MtrunA17_Chr5g0444551 49.306 144 72 1 1 144 1 143 4.48e-44 144
Msa0276380 MtrunA17_Chr5g0444571 51.579 95 45 1 1 95 1 94 1.03e-28 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0276380 AT5G42570.1 64.593 209 73 1 1 209 1 208 4.32e-97 282
Msa0276380 AT1G11905.1 59.524 210 84 1 1 210 1 209 1.07e-84 251
Msa0276380 AT1G11905.2 55.714 210 91 2 1 210 1 208 4.39e-73 221
Msa0276380 AT5G48660.1 42.056 214 115 3 1 206 1 213 2.04e-44 148
Msa0276380 AT3G07190.1 41.121 214 117 3 1 206 1 213 3.04e-42 142
Msa0276380 AT3G20450.1 50.376 133 65 1 1 132 5 137 1.08e-37 128
Msa0276380 AT5G48660.2 37.226 137 79 1 77 206 1 137 4.29e-19 80.9

Find 70 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCACACACACAGAGTTTA+TGG 0.163535 2_3:-8636853 None:intergenic
ACACTTTGTTAGGACAAAAT+TGG 0.172115 2_3:-8639660 None:intergenic
TGTTTCTCACATATTCTTCT+TGG 0.219887 2_3:-8639511 None:intergenic
TCCTACCGATCAGGTTCTTA+TGG 0.252341 2_3:+8637187 Msa0276380:CDS
CATAGCAGCTTGCTAGTAAC+AGG 0.279190 2_3:+8639574 Msa0276380:three_prime_UTR
TTATCATTGTTTCTTCTTTG+TGG 0.284036 2_3:+8639838 Msa0276380:three_prime_UTR
GTAGCTGGTTGCGGAGGTTT+TGG 0.307160 2_3:-8639455 None:intergenic
TACACCGATCAAGAGTGTTT+TGG 0.308796 2_3:-8636921 None:intergenic
ATTGAGGAAGCTTGTAATAA+TGG 0.322813 2_3:+8637025 Msa0276380:CDS
CTATCATAAGTGCAAGAAAA+AGG 0.327998 2_3:-8639152 None:intergenic
TTCATGAGAAGCATGTTTAC+AGG 0.331687 2_3:-8639606 None:intergenic
GGGGCTGAGGATGCGAAAGC+TGG 0.347270 2_3:+8639249 Msa0276380:CDS
GAATATGTGAGAAACATTTA+AGG 0.355673 2_3:+8639518 Msa0276380:exon
GAAGCTTGTAATAATGGCTT+TGG 0.364769 2_3:+8637031 Msa0276380:CDS
CCATGCTCTTTCTCCGAATC+CGG 0.367578 2_3:-8639203 None:intergenic
CACTTTGTTAGGACAAAATT+GGG 0.372520 2_3:-8639659 None:intergenic
TTATTACAAGCTTCCTCAAT+GGG 0.391866 2_3:-8637022 None:intergenic
AAGCCAGTGTAAGTGCTCTA+AGG 0.421670 2_3:+8639382 Msa0276380:CDS
ATGGGGTGAGTGAGAGGTTG+TGG 0.423024 2_3:-8636834 None:intergenic
CTTCTGTTCCTTCATTATGA+AGG 0.425413 2_3:+8639760 Msa0276380:three_prime_UTR
TAAACATGCTTCTCATGAAT+AGG 0.439713 2_3:+8639610 Msa0276380:three_prime_UTR
TTCTGTTAATCCTACCGATC+AGG 0.442382 2_3:+8637178 Msa0276380:CDS
ATCACACACACAGAGTTTAT+GGG 0.442629 2_3:-8636852 None:intergenic
ACAGACTGCATCATTACATA+AGG 0.453424 2_3:+8639175 Msa0276380:CDS
ACATAAGGGAACTCCGGATT+CGG 0.458900 2_3:+8639190 Msa0276380:CDS
ACGAGGTCGTGGACCTGTTA+TGG 0.464283 2_3:+8637064 Msa0276380:CDS
TTCTGTCAACAGATTGTAGC+TGG 0.470135 2_3:-8639470 None:intergenic
ATTATTACAAGCTTCCTCAA+TGG 0.471169 2_3:-8637023 None:intergenic
CTGTTACTAGCAAGCTGCTA+TGG 0.480052 2_3:-8639573 None:intergenic
GAGAGCAGAAGTTGATCGTC+TGG 0.490422 2_3:+8639317 Msa0276380:CDS
AGATATCAAGCACTATTTCA+AGG 0.505263 2_3:-8639789 None:intergenic
CAACAGATTGTAGCTGGTTG+CGG 0.507849 2_3:-8639464 None:intergenic
GTTATGGTGAAAACCGTTGC+TGG 0.514910 2_3:+8637080 Msa0276380:CDS
GGCACATTCCTTCATAATGA+AGG 0.518873 2_3:-8639768 None:intergenic
TTCAGATGAAACTAAAACAA+TGG 0.522908 2_3:+8639275 Msa0276380:CDS
GATCGGTTGAAACGAGGTCG+TGG 0.523142 2_3:+8637053 Msa0276380:CDS
ACTTCAGTCAAAAGATAAAG+AGG 0.526615 2_3:+8639347 Msa0276380:CDS
GTCAATATAATAGAACTCTC+TGG 0.527974 2_3:-8639875 None:intergenic
CAACAACGTGGGATCGAAGA+TGG 0.533524 2_3:+8637152 Msa0276380:CDS
TGAATATGATCGCTTACTTG+AGG 0.542275 2_3:+8639428 Msa0276380:CDS
TTGTAATAATGGCTTTGGAT+CGG 0.542465 2_3:+8637036 Msa0276380:CDS
AGCTGTTAAGAAACAAATGC+GGG 0.550293 2_3:+8639227 Msa0276380:CDS
TCTTCTTGAAGCTACACTCA+TGG 0.550488 2_3:+8637217 Msa0276380:CDS
TCATGAGAAGCATGTTTACA+GGG 0.551926 2_3:-8639605 None:intergenic
GAGTTTATGGGGTGAGTGAG+AGG 0.552067 2_3:-8636840 None:intergenic
CAGACTGCATCATTACATAA+GGG 0.555282 2_3:+8639176 Msa0276380:CDS
CTTCTTGAAGCTACACTCAT+GGG 0.557141 2_3:+8637218 Msa0276380:CDS
CAGATTGTAGCTGGTTGCGG+AGG 0.567147 2_3:-8639461 None:intergenic
GCTCTAAGGAAACAATCTGA+AGG 0.569864 2_3:+8639396 Msa0276380:CDS
ATCATTACATAAGGGAACTC+CGG 0.575747 2_3:+8639184 Msa0276380:CDS
GCCATAAGAACCTGATCGGT+AGG 0.586898 2_3:-8637188 None:intergenic
CTCTAAGGAAACAATCTGAA+GGG 0.587548 2_3:+8639397 Msa0276380:CDS
CAGTCAAAAGATAAAGAGGT+AGG 0.596716 2_3:+8639351 Msa0276380:CDS
CCGGATTCGGAGAAAGAGCA+TGG 0.597596 2_3:+8639203 Msa0276380:CDS
GCTTTGGATCGGTTGAAACG+AGG 0.607033 2_3:+8637047 Msa0276380:CDS
AGATCCAAAACACTCTTGAT+CGG 0.608818 2_3:+8636917 Msa0276380:five_prime_UTR
TTTCCTTAGAGCACTTACAC+TGG 0.609208 2_3:-8639385 None:intergenic
CTTAGCCATAAGAACCTGAT+CGG 0.613122 2_3:-8637192 None:intergenic
AACAAGAACAGTACCAGCAA+CGG 0.621936 2_3:-8637093 None:intergenic
TCACACACACAGAGTTTATG+GGG 0.626002 2_3:-8636851 None:intergenic
CTGTTAAGAAACAAATGCGG+GGG 0.642686 2_3:+8639229 Msa0276380:CDS
TGTTATTCAAGACCCCATTG+AGG 0.652558 2_3:+8637009 Msa0276380:CDS
GAATTGCTTCATCACCGACA+AGG 0.655249 2_3:+8636892 Msa0276380:five_prime_UTR
TGATGAAGATTCAACAACGT+GGG 0.655476 2_3:+8637141 Msa0276380:CDS
TATGTGAGAAACATTTAAGG+CGG 0.655679 2_3:+8639521 Msa0276380:exon
GCTGTTAAGAAACAAATGCG+GGG 0.673517 2_3:+8639228 Msa0276380:CDS
TATTACAAGCTTCCTCAATG+GGG 0.687342 2_3:-8637021 None:intergenic
AAGCTGTTAAGAAACAAATG+CGG 0.709469 2_3:+8639226 Msa0276380:CDS
TGTTAAGAAACAAATGCGGG+GGG 0.715839 2_3:+8639230 Msa0276380:CDS
ATGATGAAGATTCAACAACG+TGG 0.740230 2_3:+8637140 Msa0276380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCATAATATGAAATAAAA+GGG - chr2_3:8638113-8638132 None:intergenic 10.0%
!! TATTCATAATATGAAATAAA+AGG - chr2_3:8638114-8638133 None:intergenic 10.0%
!! TTTAATAAGTCTAAATATTA+AGG - chr2_3:8639072-8639091 None:intergenic 10.0%
!!! TTGAATTTGATTTATGTTTA+TGG + chr2_3:8637322-8637341 Msa0276380:intron 15.0%
!!! TTTTGTAAATATGATCAAAT+AGG + chr2_3:8637421-8637440 Msa0276380:intron 15.0%
!!! TTTTACTAGTTGTATATAAT+TGG + chr2_3:8638062-8638081 Msa0276380:intron 15.0%
!! TATAGTATTAAACTTATTAG+TGG + chr2_3:8638194-8638213 Msa0276380:intron 15.0%
!!! GTGTGAATTTTTTTTTTTTT+TGG + chr2_3:8639679-8639698 Msa0276380:three_prime_UTR 15.0%
!!! ATGGGTATGTTTTTTTTAAA+TGG + chr2_3:8637236-8637255 Msa0276380:intron 20.0%
!!! TGGGTATGTTTTTTTTAAAT+GGG + chr2_3:8637237-8637256 Msa0276380:intron 20.0%
!! TTGATTTATGTTTATGGATT+TGG + chr2_3:8637328-8637347 Msa0276380:intron 20.0%
!!! ATTTTGCTTATAATTAAGAC+TGG + chr2_3:8637465-8637484 Msa0276380:intron 20.0%
!!! GTTATTGTTTTACTATTGAT+AGG - chr2_3:8638042-8638061 None:intergenic 20.0%
!!! AGTATTTCTTTTGAATAGTA+TGG + chr2_3:8638169-8638188 Msa0276380:intron 20.0%
!! AATCATAAGATATATGCATA+TGG - chr2_3:8638432-8638451 None:intergenic 20.0%
!! TTCTAAACCATTGAAAAAAT+CGG - chr2_3:8638660-8638679 None:intergenic 20.0%
!! ATTGTTTATTGATGAGTTAT+TGG + chr2_3:8638766-8638785 Msa0276380:intron 20.0%
!!! CTATATGATTTGCAATTTTT+AGG + chr2_3:8638821-8638840 Msa0276380:intron 20.0%
!!! TCTTTGATTTTTAATAAGAC+CGG + chr2_3:8638958-8638977 Msa0276380:intron 20.0%
!!! TTTTACTTCAGAGTTAAATT+TGG + chr2_3:8639635-8639654 Msa0276380:three_prime_UTR 20.0%
!! AAAAAAATTCACACTTTGTT+AGG - chr2_3:8639673-8639692 None:intergenic 20.0%
!! GAATGAATGAATTTTGTATG+AGG + chr2_3:8637297-8637316 Msa0276380:intron 25.0%
! CTACATTAATGAAAGGAAAA+GGG - chr2_3:8637694-8637713 None:intergenic 25.0%
! ACTACATTAATGAAAGGAAA+AGG - chr2_3:8637695-8637714 None:intergenic 25.0%
!!! GAAGTTTTGAGTAATGTTTA+AGG + chr2_3:8638140-8638159 Msa0276380:intron 25.0%
!! AATCATTGTGATTCTTTTTC+AGG - chr2_3:8638378-8638397 None:intergenic 25.0%
! AAGTTTAACAACATACTAGA+TGG - chr2_3:8638870-8638889 None:intergenic 25.0%
! TTTGGAAATATGTTGAACTT+AGG - chr2_3:8638917-8638936 None:intergenic 25.0%
!!! CAGCAATGTTTTGAAAAATT+TGG - chr2_3:8638935-8638954 None:intergenic 25.0%
! TTATTAAAAGATATCCTCTG+TGG + chr2_3:8639084-8639103 Msa0276380:intron 25.0%
! TTCAGATGAAACTAAAACAA+TGG + chr2_3:8639275-8639294 Msa0276380:CDS 25.0%
! GAATATGTGAGAAACATTTA+AGG + chr2_3:8639518-8639537 Msa0276380:exon 25.0%
!! TTCTTCTCTTCTATACTTTT+TGG + chr2_3:8639545-8639564 Msa0276380:three_prime_UTR 25.0%
!!! TCTTCTCTTCTATACTTTTT+GGG + chr2_3:8639546-8639565 Msa0276380:three_prime_UTR 25.0%
!! TTATCATTGTTTCTTCTTTG+TGG + chr2_3:8639838-8639857 Msa0276380:three_prime_UTR 25.0%
TTATTACAAGCTTCCTCAAT+GGG - chr2_3:8637025-8637044 None:intergenic 30.0%
ATTATTACAAGCTTCCTCAA+TGG - chr2_3:8637026-8637045 None:intergenic 30.0%
ATTGAGGAAGCTTGTAATAA+TGG + chr2_3:8637025-8637044 Msa0276380:CDS 30.0%
!! TTGTAATAATGGCTTTGGAT+CGG + chr2_3:8637036-8637055 Msa0276380:CDS 30.0%
TGCTTATAATTAAGACTGGA+CGG + chr2_3:8637469-8637488 Msa0276380:intron 30.0%
! TGATCAATCACAAAACCTTT+TGG + chr2_3:8637609-8637628 Msa0276380:intron 30.0%
AGATCTTCTCAATTTCCAAA+AGG - chr2_3:8637627-8637646 None:intergenic 30.0%
AGATCGACTACATTAATGAA+AGG - chr2_3:8637701-8637720 None:intergenic 30.0%
!!! GATTCTTTTTCAGGAAAACA+CGG - chr2_3:8638369-8638388 None:intergenic 30.0%
ACAATGATTCAGCATTCATA+AGG + chr2_3:8638389-8638408 Msa0276380:intron 30.0%
TTGTTTGCCTAATTCTTTGT+TGG + chr2_3:8638600-8638619 Msa0276380:intron 30.0%
TGTTTGCCTAATTCTTTGTT+GGG + chr2_3:8638601-8638620 Msa0276380:intron 30.0%
GAATAACCCAACAAAGAATT+AGG - chr2_3:8638610-8638629 None:intergenic 30.0%
!!! TTTGCTACCGATTTTTTCAA+TGG + chr2_3:8638650-8638669 Msa0276380:intron 30.0%
! ATGGTTTAGAATGAATGTTG+AGG + chr2_3:8638669-8638688 Msa0276380:intron 30.0%
AAGTTGAAAAATGTGAGCTT+TGG + chr2_3:8638720-8638739 Msa0276380:intron 30.0%
GTTTATTGATGAGTTATTGG+TGG + chr2_3:8638769-8638788 Msa0276380:intron 30.0%
!!! ATGTTGTTTTAAGATGTGAC+CGG - chr2_3:8638980-8638999 None:intergenic 30.0%
TTAAAACAACATAGCTGAAG+AGG + chr2_3:8638988-8639007 Msa0276380:intron 30.0%
CAGTATTGTTTATGTTGGAT+TGG - chr2_3:8639027-8639046 None:intergenic 30.0%
CTCAACAGTATTGTTTATGT+TGG - chr2_3:8639032-8639051 None:intergenic 30.0%
ATTCTTGGTCAATTATTTGC+AGG + chr2_3:8639123-8639142 Msa0276380:intron 30.0%
CTATCATAAGTGCAAGAAAA+AGG - chr2_3:8639155-8639174 None:intergenic 30.0%
AAGCTGTTAAGAAACAAATG+CGG + chr2_3:8639226-8639245 Msa0276380:CDS 30.0%
ACTTCAGTCAAAAGATAAAG+AGG + chr2_3:8639347-8639366 Msa0276380:CDS 30.0%
TGTTTCTCACATATTCTTCT+TGG - chr2_3:8639514-8639533 None:intergenic 30.0%
TATGTGAGAAACATTTAAGG+CGG + chr2_3:8639521-8639540 Msa0276380:exon 30.0%
TAAACATGCTTCTCATGAAT+AGG + chr2_3:8639610-8639629 Msa0276380:three_prime_UTR 30.0%
CACTTTGTTAGGACAAAATT+GGG - chr2_3:8639662-8639681 None:intergenic 30.0%
ACACTTTGTTAGGACAAAAT+TGG - chr2_3:8639663-8639682 None:intergenic 30.0%
AGATATCAAGCACTATTTCA+AGG - chr2_3:8639792-8639811 None:intergenic 30.0%
ATCACACACACAGAGTTTAT+GGG - chr2_3:8636855-8636874 None:intergenic 35.0%
!! TGTTTTGGATCTTACCTTGT+CGG - chr2_3:8636909-8636928 None:intergenic 35.0%
AGATCCAAAACACTCTTGAT+CGG + chr2_3:8636917-8636936 Msa0276380:five_prime_UTR 35.0%
TATTACAAGCTTCCTCAATG+GGG - chr2_3:8637024-8637043 None:intergenic 35.0%
GAAGCTTGTAATAATGGCTT+TGG + chr2_3:8637031-8637050 Msa0276380:CDS 35.0%
ATGATGAAGATTCAACAACG+TGG + chr2_3:8637140-8637159 Msa0276380:CDS 35.0%
TGATGAAGATTCAACAACGT+GGG + chr2_3:8637141-8637160 Msa0276380:CDS 35.0%
! GTCATATCTAGTTTCCGTAT+CGG - chr2_3:8637516-8637535 None:intergenic 35.0%
TTTGTATGAGTTGTGAGTGT+TGG + chr2_3:8637567-8637586 Msa0276380:intron 35.0%
! GAACCCATTTTCTGATCTTT+CGG + chr2_3:8637659-8637678 Msa0276380:intron 35.0%
ACTCCGAAAGATCAGAAAAT+GGG - chr2_3:8637665-8637684 None:intergenic 35.0%
AAGAACCATTCGAGAATTGA+AGG - chr2_3:8637835-8637854 None:intergenic 35.0%
CTGTTGTCAATGCAGAAATA+CGG - chr2_3:8638255-8638274 None:intergenic 35.0%
TTCAGCATTCATAAGGCATA+AGG + chr2_3:8638396-8638415 Msa0276380:intron 35.0%
!!! TTGGTATTTTAGCCTCCTAA+TGG + chr2_3:8638501-8638520 Msa0276380:intron 35.0%
!!! TGGTATTTTAGCCTCCTAAT+GGG + chr2_3:8638502-8638521 Msa0276380:intron 35.0%
GAGAATGTTCTACTTTCTTG+TGG + chr2_3:8638742-8638761 Msa0276380:intron 35.0%
TTGCGAGCATTATTCATTCT+TGG + chr2_3:8639108-8639127 Msa0276380:intron 35.0%
ACAGACTGCATCATTACATA+AGG + chr2_3:8639175-8639194 Msa0276380:CDS 35.0%
CAGACTGCATCATTACATAA+GGG + chr2_3:8639176-8639195 Msa0276380:CDS 35.0%
ATCATTACATAAGGGAACTC+CGG + chr2_3:8639184-8639203 Msa0276380:CDS 35.0%
AGCTGTTAAGAAACAAATGC+GGG + chr2_3:8639227-8639246 Msa0276380:CDS 35.0%
CAGTCAAAAGATAAAGAGGT+AGG + chr2_3:8639351-8639370 Msa0276380:CDS 35.0%
! CTCTAAGGAAACAATCTGAA+GGG + chr2_3:8639397-8639416 Msa0276380:CDS 35.0%
TGAATATGATCGCTTACTTG+AGG + chr2_3:8639428-8639447 Msa0276380:CDS 35.0%
! TCATGAGAAGCATGTTTACA+GGG - chr2_3:8639608-8639627 None:intergenic 35.0%
! TTCATGAGAAGCATGTTTAC+AGG - chr2_3:8639609-8639628 None:intergenic 35.0%
CTTCTGTTCCTTCATTATGA+AGG + chr2_3:8639760-8639779 Msa0276380:three_prime_UTR 35.0%
TCACACACACAGAGTTTATG+GGG - chr2_3:8636854-8636873 None:intergenic 40.0%
CATCACACACACAGAGTTTA+TGG - chr2_3:8636856-8636875 None:intergenic 40.0%
!!! TACACCGATCAAGAGTGTTT+TGG - chr2_3:8636924-8636943 None:intergenic 40.0%
TGTTATTCAAGACCCCATTG+AGG + chr2_3:8637009-8637028 Msa0276380:CDS 40.0%
AACAAGAACAGTACCAGCAA+CGG - chr2_3:8637096-8637115 None:intergenic 40.0%
TTCTGTTAATCCTACCGATC+AGG + chr2_3:8637178-8637197 Msa0276380:CDS 40.0%
CTTAGCCATAAGAACCTGAT+CGG - chr2_3:8637195-8637214 None:intergenic 40.0%
TCTTCTTGAAGCTACACTCA+TGG + chr2_3:8637217-8637236 Msa0276380:CDS 40.0%
CTTCTTGAAGCTACACTCAT+GGG + chr2_3:8637218-8637237 Msa0276380:CDS 40.0%
GACTCCGAAAGATCAGAAAA+TGG - chr2_3:8637666-8637685 None:intergenic 40.0%
AATACACGTTCACGAGGTAT+AGG - chr2_3:8638341-8638360 None:intergenic 40.0%
GCTAAAATACCAAGAGTGAC+CGG - chr2_3:8638494-8638513 None:intergenic 40.0%
AATCATATGAGCCCATTAGG+AGG - chr2_3:8638516-8638535 None:intergenic 40.0%
CTGAATCATATGAGCCCATT+AGG - chr2_3:8638519-8638538 None:intergenic 40.0%
AATTAGGCAAACAACCAACG+AGG - chr2_3:8638594-8638613 None:intergenic 40.0%
! AGTTATTGGTGGCATTAGCA+GGG + chr2_3:8638780-8638799 Msa0276380:intron 40.0%
GCTGTTAAGAAACAAATGCG+GGG + chr2_3:8639228-8639247 Msa0276380:CDS 40.0%
CTGTTAAGAAACAAATGCGG+GGG + chr2_3:8639229-8639248 Msa0276380:CDS 40.0%
TGTTAAGAAACAAATGCGGG+GGG + chr2_3:8639230-8639249 Msa0276380:CDS 40.0%
! TTTCCTTAGAGCACTTACAC+TGG - chr2_3:8639388-8639407 None:intergenic 40.0%
!! GCTCTAAGGAAACAATCTGA+AGG + chr2_3:8639396-8639415 Msa0276380:CDS 40.0%
TTCTGTCAACAGATTGTAGC+TGG - chr2_3:8639473-8639492 None:intergenic 40.0%
GGCACATTCCTTCATAATGA+AGG - chr2_3:8639771-8639790 None:intergenic 40.0%
GAATTGCTTCATCACCGACA+AGG + chr2_3:8636892-8636911 Msa0276380:five_prime_UTR 45.0%
! GCAACGGTTTTCACCATAAC+AGG - chr2_3:8637080-8637099 None:intergenic 45.0%
GTTATGGTGAAAACCGTTGC+TGG + chr2_3:8637080-8637099 Msa0276380:CDS 45.0%
TCCTACCGATCAGGTTCTTA+TGG + chr2_3:8637187-8637206 Msa0276380:CDS 45.0%
! TCTAGTTTCCGTATCGGATC+CGG - chr2_3:8637510-8637529 None:intergenic 45.0%
!! TGAGTTGTGAGTGTTGGTCA+CGG + chr2_3:8637573-8637592 Msa0276380:intron 45.0%
TCTTGAGTTTACTGTGCCTC+TGG + chr2_3:8637851-8637870 Msa0276380:intron 45.0%
TTTGACAGGGTAAAGCCTCT+CGG - chr2_3:8637890-8637909 None:intergenic 45.0%
AGAGGCTTTACCCTGTCAAA+CGG + chr2_3:8637890-8637909 Msa0276380:intron 45.0%
! TTTTGACAACAGAGGACAGG+AGG - chr2_3:8637919-8637938 None:intergenic 45.0%
CTGTCCTCTGTTGTCAAAAG+CGG + chr2_3:8637920-8637939 Msa0276380:intron 45.0%
CGGAGCATAGCAGAAAAATC+AGG + chr2_3:8637961-8637980 Msa0276380:intron 45.0%
GTCAATGCAGAAATACGGTG+TGG - chr2_3:8638250-8638269 None:intergenic 45.0%
TCTAGAAGCTGAACCTAGAC+AGG - chr2_3:8638282-8638301 None:intergenic 45.0%
GTTCGAAATACACGTTCACG+AGG - chr2_3:8638347-8638366 None:intergenic 45.0%
! GAGTTATTGGTGGCATTAGC+AGG + chr2_3:8638779-8638798 Msa0276380:intron 45.0%
ACATAAGGGAACTCCGGATT+CGG + chr2_3:8639190-8639209 Msa0276380:CDS 45.0%
AAGCCAGTGTAAGTGCTCTA+AGG + chr2_3:8639382-8639401 Msa0276380:CDS 45.0%
CAACAGATTGTAGCTGGTTG+CGG - chr2_3:8639467-8639486 None:intergenic 45.0%
CTGTTACTAGCAAGCTGCTA+TGG - chr2_3:8639576-8639595 None:intergenic 45.0%
!! CATAGCAGCTTGCTAGTAAC+AGG + chr2_3:8639574-8639593 Msa0276380:three_prime_UTR 45.0%
GAGTTTATGGGGTGAGTGAG+AGG - chr2_3:8636843-8636862 None:intergenic 50.0%
! GCTTTGGATCGGTTGAAACG+AGG + chr2_3:8637047-8637066 Msa0276380:CDS 50.0%
CAACAACGTGGGATCGAAGA+TGG + chr2_3:8637152-8637171 Msa0276380:CDS 50.0%
GCCATAAGAACCTGATCGGT+AGG - chr2_3:8637191-8637210 None:intergenic 50.0%
! ACGGAGTATCTGTCAAGCAC+CGG + chr2_3:8637488-8637507 Msa0276380:intron 50.0%
TGACACTGACACAGACACGT+TGG + chr2_3:8637532-8637551 Msa0276380:intron 50.0%
TTGCTGCATCTGATGCAGCA+TGG + chr2_3:8637806-8637825 Msa0276380:intron 50.0%
GGCTGCCTTCAATTCTCGAA+TGG + chr2_3:8637827-8637846 Msa0276380:intron 50.0%
GAGGCTTTACCCTGTCAAAC+GGG + chr2_3:8637891-8637910 Msa0276380:intron 50.0%
! CGCTTTTGACAACAGAGGAC+AGG - chr2_3:8637922-8637941 None:intergenic 50.0%
TCTGCTATGCTCCGCTATAG+CGG - chr2_3:8637955-8637974 None:intergenic 50.0%
CGGGTATCAGAAGTGGAAAG+CGG + chr2_3:8638007-8638026 Msa0276380:intron 50.0%
AGAAGTGGAAAGCGGCAAAG+CGG + chr2_3:8638015-8638034 Msa0276380:intron 50.0%
AAATACGGTGTGGCGCATAG+CGG - chr2_3:8638240-8638259 None:intergenic 50.0%
TGACAACAGAGCTCCTGTCT+AGG + chr2_3:8638266-8638285 Msa0276380:intron 50.0%
AGAGAAAGAGCCTTCACCCT+TGG + chr2_3:8638463-8638482 Msa0276380:intron 50.0%
GAGAAAGAGCCTTCACCCTT+GGG + chr2_3:8638464-8638483 Msa0276380:intron 50.0%
! AGAGTGACCGGTTTACCCAA+GGG - chr2_3:8638482-8638501 None:intergenic 50.0%
! AAGAGTGACCGGTTTACCCA+AGG - chr2_3:8638483-8638502 None:intergenic 50.0%
TTGGGTAAACCGGTCACTCT+TGG + chr2_3:8638482-8638501 Msa0276380:intron 50.0%
ACTTGTGAGTGAAGCCTCGT+TGG + chr2_3:8638577-8638596 Msa0276380:intron 50.0%
ATAATGCTCGCAAGCCACAG+AGG - chr2_3:8639101-8639120 None:intergenic 50.0%
CCATGCTCTTTCTCCGAATC+CGG - chr2_3:8639206-8639225 None:intergenic 50.0%
! GAGAGCAGAAGTTGATCGTC+TGG + chr2_3:8639317-8639336 Msa0276380:CDS 50.0%
GATCGGTTGAAACGAGGTCG+TGG + chr2_3:8637053-8637072 Msa0276380:CDS 55.0%
ACGAGGTCGTGGACCTGTTA+TGG + chr2_3:8637064-8637083 Msa0276380:CDS 55.0%
!! GTCAAGCACCGGATCCGATA+CGG + chr2_3:8637499-8637518 Msa0276380:intron 55.0%
AGGGTAAAGCCTCTCGGACT+GGG - chr2_3:8637884-8637903 None:intergenic 55.0%
AAAATCAGGACCACTGCGCC+AGG + chr2_3:8637975-8637994 Msa0276380:intron 55.0%
AATACGGTGTGGCGCATAGC+GGG - chr2_3:8638239-8638258 None:intergenic 55.0%
!! ACTGGAAGAGGTGCCAACAG+GGG - chr2_3:8638317-8638336 None:intergenic 55.0%
!! CACTGGAAGAGGTGCCAACA+GGG - chr2_3:8638318-8638337 None:intergenic 55.0%
!! CGAGGTATAGGCACTGGAAG+AGG - chr2_3:8638329-8638348 None:intergenic 55.0%
CGTTCACGAGGTATAGGCAC+TGG - chr2_3:8638335-8638354 None:intergenic 55.0%
GCCTTCACCCTTGGGTAAAC+CGG + chr2_3:8638472-8638491 Msa0276380:intron 55.0%
! ACCGGTTTACCCAAGGGTGA+AGG - chr2_3:8638476-8638495 None:intergenic 55.0%
CCGGATTCGGAGAAAGAGCA+TGG + chr2_3:8639203-8639222 Msa0276380:CDS 55.0%
! GTAGCTGGTTGCGGAGGTTT+TGG - chr2_3:8639458-8639477 None:intergenic 55.0%
CAGATTGTAGCTGGTTGCGG+AGG - chr2_3:8639464-8639483 None:intergenic 55.0%
GGGTAAAGCCTCTCGGACTG+GGG - chr2_3:8637883-8637902 None:intergenic 60.0%
CAGGGTAAAGCCTCTCGGAC+TGG - chr2_3:8637885-8637904 None:intergenic 60.0%
CAGGAGGAGCCCGTTTGACA+GGG - chr2_3:8637903-8637922 None:intergenic 60.0%
ACAGGAGGAGCCCGTTTGAC+AGG - chr2_3:8637904-8637923 None:intergenic 60.0%
!!! GCGGCCGCTTTTGACAACAG+AGG - chr2_3:8637927-8637946 None:intergenic 60.0%
GTTGTCAAAAGCGGCCGCAC+CGG + chr2_3:8637929-8637948 Msa0276380:intron 60.0%
CTATGCTCCGCTATAGCGGC+CGG - chr2_3:8637951-8637970 None:intergenic 60.0%
ACTTCTGATACCCGCCGACG+CGG - chr2_3:8638001-8638020 None:intergenic 60.0%
!! CATAGCGGGCGCTATTTCGG+TGG - chr2_3:8638225-8638244 None:intergenic 60.0%
!! GCGCATAGCGGGCGCTATTT+CGG - chr2_3:8638228-8638247 None:intergenic 60.0%
ACCTCTCTCAGCTCCCCTGT+TGG + chr2_3:8638301-8638320 Msa0276380:intron 60.0%
!! GCACTGGAAGAGGTGCCAAC+AGG - chr2_3:8638319-8638338 None:intergenic 60.0%
GAAACAAATGCGGGGGGCTG+AGG + chr2_3:8639236-8639255 Msa0276380:CDS 60.0%
! CGGACTGGGGAAAGAGCCAG+AGG - chr2_3:8637870-8637889 None:intergenic 65.0%
GGCTCTTTCCCCAGTCCGAG+AGG + chr2_3:8637872-8637891 Msa0276380:intron 65.0%
! GCGTCGGCGGGTATCAGAAG+TGG + chr2_3:8638000-8638019 Msa0276380:intron 65.0%
GCCAACAGGGGAGCTGAGAG+AGG - chr2_3:8638305-8638324 None:intergenic 65.0%
GGGGCTGAGGATGCGAAAGC+TGG + chr2_3:8639249-8639268 Msa0276380:CDS 65.0%
CTCCGCTATAGCGGCCGGTG+CGG - chr2_3:8637946-8637965 None:intergenic 70.0%
! GGCCGCACCGGCCGCTATAG+CGG + chr2_3:8637941-8637960 Msa0276380:intron 75.0%
ACCACTGCGCCAGGCCGCGT+CGG + chr2_3:8637984-8638003 Msa0276380:intron 75.0%
TGATACCCGCCGACGCGGCC+TGG - chr2_3:8637996-8638015 None:intergenic 75.0%
! ACTGCGCCAGGCCGCGTCGG+CGG + chr2_3:8637987-8638006 Msa0276380:intron 80.0%
! GCCGACGCGGCCTGGCGCAG+TGG - chr2_3:8637988-8638007 None:intergenic 85.0%
!! CTGCGCCAGGCCGCGTCGGC+GGG + chr2_3:8637988-8638007 Msa0276380:intron 85.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 8636835 8639887 8636835 ID=Msa0276380;Name=Msa0276380
chr2_3 mRNA 8636835 8639887 8636835 ID=Msa0276380-mRNA-1;Parent=Msa0276380;Name=Msa0276380-mRNA-1;_AED=0.11;_eAED=0.11;_QI=119|1|1|1|0|1|2|360|222
chr2_3 exon 8636835 8637239 8636835 ID=Msa0276380-mRNA-1:exon:3888;Parent=Msa0276380-mRNA-1
chr2_3 exon 8639145 8639887 8639145 ID=Msa0276380-mRNA-1:exon:3889;Parent=Msa0276380-mRNA-1
chr2_3 five_prime_UTR 8636835 8636953 8636835 ID=Msa0276380-mRNA-1:five_prime_utr;Parent=Msa0276380-mRNA-1
chr2_3 CDS 8636954 8637239 8636954 ID=Msa0276380-mRNA-1:cds;Parent=Msa0276380-mRNA-1
chr2_3 CDS 8639145 8639527 8639145 ID=Msa0276380-mRNA-1:cds;Parent=Msa0276380-mRNA-1
chr2_3 three_prime_UTR 8639528 8639887 8639528 ID=Msa0276380-mRNA-1:three_prime_utr;Parent=Msa0276380-mRNA-1
Gene Sequence

>Msa0276380

ATGATTCATCTATTATTCACAGTGATTTTTTCAGAAGCAACAATGATCGCATTGCTGTTATTCAAGACCCCATTGAGGAAGCTTGTAATAATGGCTTTGGATCGGTTGAAACGAGGTCGTGGACCTGTTATGGTGAAAACCGTTGCTGGTACTGTTCTTGTTGTTTTGTCTTCTAGTGTTTATAGTATGATGAAGATTCAACAACGTGGGATCGAAGATGGTGCTTCTGTTAATCCTACCGATCAGGTTCTTATGGCTAAGCATCTTCTTGAAGCTACACTCATGGGTGCTATCCTTTTTCTTGCACTTATGATAGACAGACTGCATCATTACATAAGGGAACTCCGGATTCGGAGAAAGAGCATGGAAGCTGTTAAGAAACAAATGCGGGGGGCTGAGGATGCGAAAGCTGGAACTTCAGATGAAACTAAAACAATGGAGCAAGAAGCAGCTAAGTTGAGAGCAGAAGTTGATCGTCTGGAATCTGAACTTCAGTCAAAAGATAAAGAGGTAGGTGCTGCAGAAGCCAGTGTAAGTGCTCTAAGGAAACAATCTGAAGGGTTTCTTCTTGAATATGATCGCTTACTTGAGGAGAACCAAAACCTCCGCAACCAGCTACAATCTGTTGACAGAAGATTCTCACATTCAGTTTCCAAGAAGAATATGTGA
Protein sequence

>Msa0276380

MIHLLFTVIFSEATMIALLLFKTPLRKLVIMALDRLKRGRGPVMVKTVAGTVLVVLSSSVYSMMKIQQRGIEDGASVNPTDQVLMAKHLLEATLMGAILFLALMIDRLHHYIRELRIRRKSMEAVKKQMRGAEDAKAGTSDETKTMEQEAAKLRAEVDRLESELQSKDKEVGAAEASVSALRKQSEGFLLEYDRLLEENQNLRNQLQSVDRRFSHSVSKKNM*