AlfalfaGEDB | Msa0238660

M. sativa cultivar zhongmu-4 / Msa0238660

KEGG: K14009
GO: GO:0000003 GO:0000139 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0005795 GO:0005811 GO:0005886 GO:0005887 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006888 GO:0007154 GO:0007165 GO:0007204 GO:0007276 GO:0007283 GO:0008104 GO:0008150 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009967 GO:0009987 GO:0010604 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012505 GO:0016020 GO:0016021 GO:0016192 GO:0019222 GO:0019722 GO:0019725 GO:0019932 GO:0019953 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030003 GO:0030135 GO:0030136 GO:0030162 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031410 GO:0031982 GO:0031984 GO:0031985 GO:0032268 GO:0032270 GO:0032386 GO:0032388 GO:0032434 GO:0032436 GO:0032469 GO:0032471 GO:0032501 GO:0032504 GO:0032580 GO:0032879 GO:0032880 GO:0033036 GO:0033157 GO:0033365 GO:0034613 GO:0035556 GO:0035584 GO:0042175 GO:0042176 GO:0042287 GO:0042288 GO:0042592 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043280 GO:0043281 GO:0044093 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0044703 GO:0044877 GO:0045732 GO:0045862 GO:0046907 GO:0048193 GO:0048232 GO:0048471 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048609 GO:0048878 GO:0050789 GO:0050790 GO:0050794 GO:0050801 GO:0050896 GO:0051049 GO:0051050 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051480 GO:0051560 GO:0051561 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0052547 GO:0052548 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0060255 GO:0060341 GO:0061136 GO:0065007 GO:0065008 GO:0065009 GO:0070201 GO:0070727 GO:0070861 GO:0070863 GO:0070972 GO:0070973 GO:0071944 GO:0072503 GO:0072507 GO:0080090 GO:0080134 GO:0080135 GO:0090087 GO:0090316 GO:0097038 GO:0097708 GO:0098588 GO:0098771 GO:0098791 GO:0098827 GO:1901800 GO:1902531 GO:1902533 GO:1903050 GO:1903052 GO:1903069 GO:1903071 GO:1903362 GO:1903364 GO:1903827 GO:1903829 GO:1904152 GO:1904154 GO:1904292 GO:1904294 GO:1904951 GO:1905897 GO:1905898 GO:2000058 GO:2000060 GO:2000116 GO:2001056 GO:2001233 GO:2001235 GO:2001242 GO:2001244
PFAM: PF17675.4

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0238660	XP_013462866.1	99.099	222	2	0	1	222	1	222	2.35e-156	442

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0238660	A0A072V5J3	99.099	222	2	0	1	222	1	222	1.12e-156	442

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0098470	Msa0238660	0.810252	1.264028e-50	-8.615850e-47
Msa0152870	Msa0238660	0.800646	1.307000e-48	-8.615850e-47
Msa0209140	Msa0238660	0.817117	3.894369e-52	-8.615850e-47
Msa0238660	Msa0276380	0.975271	1.196027e-139	-8.615850e-47
Msa0238660	Msa0296250	0.804005	2.658728e-49	-8.615850e-47
Msa0238660	Msa0309720	0.800552	1.365867e-48	-8.615850e-47
Msa0238660	Msa0316430	0.884643	1.609309e-71	-8.615850e-47
Msa0238660	Msa0435370	0.804129	2.505357e-49	-8.615850e-47
Msa0238660	Msa0435420	0.800335	1.512411e-48	-8.615850e-47
Msa0238660	Msa1206230	0.819863	9.288310e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0238660	MtrunA17_Chr2g0288831	99.099	222	2	0	1	222	1	222	2.16e-160	442
Msa0238660	MtrunA17_Chr4g0024941	61.086	221	85	1	1	221	1	220	8.06e-97	281
Msa0238660	MtrunA17_Chr2g0322421	51.584	221	106	1	1	221	1	220	1.13e-68	210
Msa0238660	MtrunA17_Chr7g0235621	44.131	213	110	3	1	205	1	212	1.30e-49	161
Msa0238660	MtrunA17_Chr5g0444551	49.306	144	72	1	1	144	1	143	4.48e-44	144
Msa0238660	MtrunA17_Chr5g0444571	51.579	95	45	1	1	95	1	94	1.03e-28	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0238660	AT5G42570.1	64.593	209	73	1	1	209	1	208	4.32e-97	282
Msa0238660	AT1G11905.1	59.524	210	84	1	1	210	1	209	1.07e-84	251
Msa0238660	AT1G11905.2	55.714	210	91	2	1	210	1	208	4.39e-73	221
Msa0238660	AT5G48660.1	42.056	214	115	3	1	206	1	213	2.04e-44	148
Msa0238660	AT3G07190.1	41.121	214	117	3	1	206	1	213	3.04e-42	142
Msa0238660	AT3G20450.1	50.376	133	65	1	1	132	5	137	1.08e-37	128
Msa0238660	AT5G48660.2	37.226	137	79	1	77	206	1	137	4.29e-19	80.9

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Find 71 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAGTGCTTGATATCTCT+TGG	0.410580	2_2:+12487522	Msa0238660:three_prime_UTR
AACAAGAACAGTACCAGCAA+CGG	0.621936	2_2:-12484821	None:intergenic
AAGCCAGTGTAAGTGCTCTA+AGG	0.421670	2_2:+12487110	Msa0238660:CDS
AAGCTGTTAAGAAACAAATG+CGG	0.709469	2_2:+12486954	Msa0238660:CDS
ACACTTTGTTAGGACAAAAT+TGG	0.172115	2_2:-12487388	None:intergenic
ACAGACTGCATCATTACATA+AGG	0.453424	2_2:+12486903	Msa0238660:CDS
ACATAAGGGAACTCCGGATT+CGG	0.458900	2_2:+12486918	Msa0238660:CDS
ACGAGGTCGTGGACCTGTTA+TGG	0.464283	2_2:+12484792	Msa0238660:CDS
ACTTCAGTCAAAAGATAAAG+AGG	0.526615	2_2:+12487075	Msa0238660:CDS
AGATATCAAGCACTATTTCA+AGG	0.505263	2_2:-12487517	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTATTACAAGCTTCCTCAAT+GGG	-	chr2_2:12484753-12484772	None:intergenic	30.0%
ATTATTACAAGCTTCCTCAA+TGG	-	chr2_2:12484754-12484773	None:intergenic	30.0%
ATTGAGGAAGCTTGTAATAA+TGG	+	chr2_2:12484753-12484772	Msa0238660:CDS	30.0%
TGCTTATAATTAAGACTGGA+CGG	+	chr2_2:12485197-12485216	Msa0238660:intron	30.0%
AGATCTTCTCAATTTCCAAA+AGG	-	chr2_2:12485355-12485374	None:intergenic	30.0%
AGATCGACTACATTAATGAA+AGG	-	chr2_2:12485429-12485448	None:intergenic	30.0%
ACAATGATTCAGCATTCATA+AGG	+	chr2_2:12486117-12486136	Msa0238660:intron	30.0%
TTGTTTGCCTAATTCTTTGT+TGG	+	chr2_2:12486328-12486347	Msa0238660:intron	30.0%
TGTTTGCCTAATTCTTTGTT+GGG	+	chr2_2:12486329-12486348	Msa0238660:intron	30.0%
GAATAACCCAACAAAGAATT+AGG	-	chr2_2:12486338-12486357	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	12484563	12487616	12484563	ID=Msa0238660;Name=Msa0238660
chr2_2	mRNA	12484563	12487616	12484563	ID=Msa0238660-mRNA-1;Parent=Msa0238660;Name=Msa0238660-mRNA-1;_AED=0.11;_eAED=0.11;_QI=119\|1\|1\|1\|0\|1\|2\|361\|222
chr2_2	exon	12484563	12484967	12484563	ID=Msa0238660-mRNA-1:exon:5254;Parent=Msa0238660-mRNA-1
chr2_2	exon	12486873	12487616	12486873	ID=Msa0238660-mRNA-1:exon:5255;Parent=Msa0238660-mRNA-1
chr2_2	five_prime_UTR	12484563	12484681	12484563	ID=Msa0238660-mRNA-1:five_prime_utr;Parent=Msa0238660-mRNA-1
chr2_2	CDS	12484682	12484967	12484682	ID=Msa0238660-mRNA-1:cds;Parent=Msa0238660-mRNA-1
chr2_2	CDS	12486873	12487255	12486873	ID=Msa0238660-mRNA-1:cds;Parent=Msa0238660-mRNA-1
chr2_2	three_prime_UTR	12487256	12487616	12487256	ID=Msa0238660-mRNA-1:three_prime_utr;Parent=Msa0238660-mRNA-1

Gene Sequence

>Msa0238660

ATGATTCATCTATTATTCACAGTGATTTTTTCAGAAGCAACAATGATCGCATTGCTGTTATTCAAGACCCCATTGAGGAAGCTTGTAATAATGGCTTTGGATCGGTTGAAACGAGGTCGTGGACCTGTTATGGTGAAAACCGTTGCTGGTACTGTTCTTGTTGTTTTGTCTTCTAGTGTTTATAGTATGATGAAGATTCAACAACGTGGGATCGAAGATGGTGCTTCTGTTAATCCTACCGATCAGGTTCTTATGGCTAAGCATCTTCTTGAAGCTACACTCATGGGTGCTATCCTTTTTCTTGCACTTATGATAGACAGACTGCATCATTACATAAGGGAACTCCGGATTCGGAGAAAGAGCATGGAAGCTGTTAAGAAACAAATGCGGGGGGCTGAGGATGCGAAAGCTGGAACTTCAGATGAAACTAAAACAATGGAGCAAGAAGCAGCTAAGTTGAGAGCAGAAGTTGATCGTCTGGAATCTGAACTTCAGTCAAAAGATAAAGAGGTAGGTGCTGCAGAAGCCAGTGTAAGTGCTCTAAGGAAACAATCTGAAGGGTTTCTTCTTGAATATGATCGCTTACTTGAGGAGAACCAAAACCTCCGCAACCAGCTACAATCTGTTGACAGAAGATTCTCACATTCAGTTTCCAAGAAGAATATGTGA

Protein sequence

>Msa0238660

MIHLLFTVIFSEATMIALLLFKTPLRKLVIMALDRLKRGRGPVMVKTVAGTVLVVLSSSVYSMMKIQQRGIEDGASVNPTDQVLMAKHLLEATLMGAILFLALMIDRLHHYIRELRIRRKSMEAVKKQMRGAEDAKAGTSDETKTMEQEAAKLRAEVDRLESELQSKDKEVGAAEASVSALRKQSEGFLLEYDRLLEENQNLRNQLQSVDRRFSHSVSKKNM*