AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0320200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320200 MtrunA17_Chr2g0295124 97.452 157 4 0 1 157 1 157 8.41e-108 303
Msa0320200 MtrunA17_Chr2g0295104 93.631 157 10 0 1 157 1 157 7.42e-104 293
Msa0320200 MtrunA17_Chr2g0295091 91.083 157 14 0 1 157 1 157 3.58e-100 284
Msa0320200 MtrunA17_Chr2g0295141 87.261 157 20 0 1 157 1 157 3.33e-97 277
Msa0320200 MtrunA17_Chr6g0462311 66.879 157 52 0 1 157 1 157 6.04e-69 205
Msa0320200 MtrunA17_Chr4g0067951 66.879 157 52 0 1 157 1 157 1.99e-68 204
Msa0320200 MtrunA17_Chr2g0295021 63.057 157 58 0 1 157 1 157 3.64e-65 196
Msa0320200 MtrunA17_Chr2g0295001 63.057 157 58 0 1 157 1 157 3.64e-65 196
Msa0320200 MtrunA17_Chr2g0295064 63.694 157 57 0 1 157 68 224 1.42e-63 194
Msa0320200 MtrunA17_Chr2g0295051 60.510 157 62 0 1 157 1 157 6.42e-62 187
Msa0320200 MtrunA17_Chr2g0295041 58.599 157 65 0 1 157 1 157 1.13e-58 179
Msa0320200 MtrunA17_Chr6g0462291 65.000 140 49 0 1 140 1 140 8.62e-58 177
Msa0320200 MtrunA17_Chr2g0295081 49.315 146 74 0 12 157 12 157 3.71e-47 150
Msa0320200 MtrunA17_Chr4g0067841 34.395 157 102 1 1 156 1 157 5.68e-31 109
Msa0320200 MtrunA17_Chr4g0067921 40.373 161 89 5 1 157 1 158 1.98e-30 108
Msa0320200 MtrunA17_Chr2g0295211 31.410 156 106 1 1 155 1 156 5.95e-30 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACATTTGCTTAATAATT+AGG 0.095843 2_4:-20482774 None:intergenic
TTGAGGTTTGAGTGTTGTTT+TGG 0.265436 2_4:+20482908 Msa0320200:three_prime_UTR
GATAATTTCTTGATGGTTCC+AGG 0.292734 2_4:-20481363 None:intergenic
ATACATTTGCTTAATAATTA+GGG 0.306692 2_4:-20482773 None:intergenic
GGCCATTGAGGGTTACGTTT+TGG 0.315356 2_4:+20482746 Msa0320200:CDS
TGGGCAGCTGGAATCACCTT+TGG 0.317415 2_4:-20481309 None:intergenic
CTTGATAAGTCCAGTTCCTT+TGG 0.324632 2_4:-20482724 None:intergenic
GGGCAGCTGGAATCACCTTT+GGG 0.327572 2_4:-20481308 None:intergenic
ATATATATATCAAGATAAAA+AGG 0.344259 2_4:-20481192 None:intergenic
TTCAACAATTTCAACACTTT+GGG 0.350751 2_4:-20481328 None:intergenic
CTTCAACAATTTCAACACTT+TGG 0.364915 2_4:-20481329 None:intergenic
AAGTCCAGTTCCTTTGGCTT+TGG 0.382566 2_4:-20482718 None:intergenic
AGAGCTTTGTAGAGTTTAGC+TGG 0.394931 2_4:-20481261 None:intergenic
GTTGAAATTGTTGAAGGAAA+TGG 0.402462 2_4:+20481336 Msa0320200:CDS
AGCAGTGGATGAGGCAAACT+TGG 0.402684 2_4:+20482527 Msa0320200:CDS
TTATCTTGATATATATATCA+TGG 0.403777 2_4:+20481197 Msa0320200:exon
TATAGCATAGTAGGAGGAAC+AGG 0.403927 2_4:+20482558 Msa0320200:CDS
ATTTCAACACTTTGGGCAGC+TGG 0.408726 2_4:-20481321 None:intergenic
AGATAGAACTGCATCACCTT+TGG 0.408990 2_4:-20482676 None:intergenic
AGACATGGATAATTTCTTGA+TGG 0.415791 2_4:-20481370 None:intergenic
GAGACCAAAGCCAAAGGAAC+TGG 0.425614 2_4:+20482714 Msa0320200:CDS
AGTGTTGTGGCTGGCTCTGA+TGG 0.429803 2_4:+20482618 Msa0320200:CDS
TTATTAAACAACCATATCAT+AGG 0.433028 2_4:-20482830 None:intergenic
GGATACAACTATAGCATAGT+AGG 0.470749 2_4:+20482549 Msa0320200:CDS
GGACTTATCAAGGCCATTGA+GGG 0.495298 2_4:+20482735 Msa0320200:CDS
CAAAGGAACTGGACTTATCA+AGG 0.497615 2_4:+20482725 Msa0320200:CDS
CTCTAGCTCGTACATGTGTG+TGG 0.498650 2_4:+20482869 Msa0320200:three_prime_UTR
TGCCAAAACGTAACCCTCAA+TGG 0.499044 2_4:-20482748 None:intergenic
CAAAGTGTTGAAATTGTTGA+AGG 0.502542 2_4:+20481330 Msa0320200:CDS
TATCTTGATATATATATCAT+GGG 0.511953 2_4:+20481198 Msa0320200:exon
CAAATTAGAAGCAGTGGATG+AGG 0.520372 2_4:+20482518 Msa0320200:CDS
TGGACTTATCAAGGCCATTG+AGG 0.566375 2_4:+20482734 Msa0320200:CDS
TTTCACTGAAATCTTAACAA+TGG 0.569031 2_4:-20482646 None:intergenic
TACTAATTTAGTTTGTGATG+CGG 0.569445 2_4:-20481121 None:intergenic
GTTGAAGGAAATGGAGGACC+TGG 0.574664 2_4:+20481345 Msa0320200:CDS
ACATGTGTGTGGGTCTATGT+TGG 0.584175 2_4:+20482880 Msa0320200:three_prime_UTR
TCTAGCTCGTACATGTGTGT+GGG 0.587397 2_4:+20482870 Msa0320200:three_prime_UTR
TAATTTGTGTAGCACAAAGT+CGG 0.587472 2_4:-20482502 None:intergenic
TTCGAGACAAGTGTTGTGGC+TGG 0.589865 2_4:+20482609 Msa0320200:CDS
AAGAAATTATCCATGTCTGA+AGG 0.597444 2_4:+20481375 Msa0320200:CDS
TAATCAATTACCTTCAGACA+TGG 0.598263 2_4:-20481385 None:intergenic
GTTGTGGCTGGCTCTGATGG+TGG 0.598616 2_4:+20482621 Msa0320200:CDS
GCTACACAAATTAGAAGCAG+TGG 0.603203 2_4:+20482512 Msa0320200:CDS
CATAGTAGGAGGAACAGGGT+TGG 0.607807 2_4:+20482563 Msa0320200:CDS
TGCTGATGAAATCATCCCAA+AGG 0.616025 2_4:+20481293 Msa0320200:CDS
ATATGCAAGAAGTAAATGAA+AGG 0.619747 2_4:-20481165 None:intergenic
GAAATTGTTGAAGGAAATGG+AGG 0.626795 2_4:+20481339 Msa0320200:CDS
GCAGTGGATGAGGCAAACTT+GGG 0.627060 2_4:+20482528 Msa0320200:CDS
TACAACTATAGCATAGTAGG+AGG 0.648903 2_4:+20482552 Msa0320200:CDS
TGAATTCGAGACAAGTGTTG+TGG 0.660471 2_4:+20482605 Msa0320200:CDS
TCAGTGAAATACCACACCAA+AGG 0.661287 2_4:+20482660 Msa0320200:CDS
CGTGAGGAGACCAAAGCCAA+AGG 0.666854 2_4:+20482708 Msa0320200:CDS
GAACTGCATCACCTTTGGTG+TGG 0.674778 2_4:-20482671 None:intergenic
TCTATCTGAAGCAGTACGTG+AGG 0.678507 2_4:+20482692 Msa0320200:CDS
ATAGCATAGTAGGAGGAACA+GGG 0.716365 2_4:+20482559 Msa0320200:CDS
TGATGAACATGTCTCAACTG+TGG 0.723859 2_4:+20481236 Msa0320200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATCAAGATAAAA+AGG - chr2_4:20481195-20481214 None:intergenic 10.0%
!! ATATATATATCAAGATAAAA+AGG - chr2_4:20481195-20481214 None:intergenic 10.0%
!! TTATCTTGATATATATATCA+TGG + chr2_4:20481197-20481216 Msa0320200:exon 15.0%
!! TATCTTGATATATATATCAT+GGG + chr2_4:20481198-20481217 Msa0320200:exon 15.0%
!!! TTTATTTTTTCCATGAAAAA+TGG - chr2_4:20481640-20481659 None:intergenic 15.0%
!! AACAAACAAATGAATAAATA+AGG + chr2_4:20481665-20481684 Msa0320200:intron 15.0%
!! ACAAACAAATGAATAAATAA+GGG + chr2_4:20481666-20481685 Msa0320200:intron 15.0%
!!! AGTACAAATTTAGAATTTTA+CGG - chr2_4:20481760-20481779 None:intergenic 15.0%
!! TAATTTCAACTATCAATTAT+AGG + chr2_4:20481935-20481954 Msa0320200:intron 15.0%
!! AGTTATTATTACATTGAAAA+TGG - chr2_4:20481961-20481980 None:intergenic 15.0%
!! CTTATTATCATTTATGTATA+TGG - chr2_4:20481999-20482018 None:intergenic 15.0%
!! AAAAAACTAAAACATTTACA+CGG + chr2_4:20482292-20482311 Msa0320200:intron 15.0%
!!! TCAAACTTTATTTACATTTA+AGG + chr2_4:20482431-20482450 Msa0320200:intron 15.0%
!! ATACATTTGCTTAATAATTA+GGG - chr2_4:20482776-20482795 None:intergenic 15.0%
!! TATACATTTGCTTAATAATT+AGG - chr2_4:20482777-20482796 None:intergenic 15.0%
!! TTATCTTGATATATATATCA+TGG + chr2_4:20481197-20481216 Msa0320200:exon 15.0%
!! TATCTTGATATATATATCAT+GGG + chr2_4:20481198-20481217 Msa0320200:exon 15.0%
!!! TTTATTTTTTCCATGAAAAA+TGG - chr2_4:20481640-20481659 None:intergenic 15.0%
!! AACAAACAAATGAATAAATA+AGG + chr2_4:20481665-20481684 Msa0320200:intron 15.0%
!! ACAAACAAATGAATAAATAA+GGG + chr2_4:20481666-20481685 Msa0320200:intron 15.0%
!!! AGTACAAATTTAGAATTTTA+CGG - chr2_4:20481760-20481779 None:intergenic 15.0%
!! TAATTTCAACTATCAATTAT+AGG + chr2_4:20481935-20481954 Msa0320200:intron 15.0%
!! AGTTATTATTACATTGAAAA+TGG - chr2_4:20481961-20481980 None:intergenic 15.0%
!! CTTATTATCATTTATGTATA+TGG - chr2_4:20481999-20482018 None:intergenic 15.0%
!! AAAAAACTAAAACATTTACA+CGG + chr2_4:20482292-20482311 Msa0320200:intron 15.0%
!!! TCAAACTTTATTTACATTTA+AGG + chr2_4:20482431-20482450 Msa0320200:intron 15.0%
!! ATACATTTGCTTAATAATTA+GGG - chr2_4:20482776-20482795 None:intergenic 15.0%
!! TATACATTTGCTTAATAATT+AGG - chr2_4:20482777-20482796 None:intergenic 15.0%
!! TTCAATTCTAGTATTTGAAT+AGG + chr2_4:20481489-20481508 Msa0320200:intron 20.0%
!! AAATAGAATTTGAGAATTGA+AGG + chr2_4:20481556-20481575 Msa0320200:intron 20.0%
!! CAAACAAATGAATAAATAAG+GGG + chr2_4:20481667-20481686 Msa0320200:intron 20.0%
!! CTAAAAATTATTTCAGTCAT+CGG - chr2_4:20482411-20482430 None:intergenic 20.0%
!! TTATTAAGCAAATGTATACT+TGG + chr2_4:20482779-20482798 Msa0320200:intron 20.0%
!! TTATTAAACAACCATATCAT+AGG - chr2_4:20482833-20482852 None:intergenic 20.0%
!! TTCAATTCTAGTATTTGAAT+AGG + chr2_4:20481489-20481508 Msa0320200:intron 20.0%
!! AAATAGAATTTGAGAATTGA+AGG + chr2_4:20481556-20481575 Msa0320200:intron 20.0%
!! CAAACAAATGAATAAATAAG+GGG + chr2_4:20481667-20481686 Msa0320200:intron 20.0%
!! CTAAAAATTATTTCAGTCAT+CGG - chr2_4:20482411-20482430 None:intergenic 20.0%
!! TTATTAAGCAAATGTATACT+TGG + chr2_4:20482779-20482798 Msa0320200:intron 20.0%
!! TTATTAAACAACCATATCAT+AGG - chr2_4:20482833-20482852 None:intergenic 20.0%
!!! TACTAATTTAGTTTGTGATG+CGG - chr2_4:20481124-20481143 None:intergenic 25.0%
! ATATGCAAGAAGTAAATGAA+AGG - chr2_4:20481168-20481187 None:intergenic 25.0%
! TTCAACAATTTCAACACTTT+GGG - chr2_4:20481331-20481350 None:intergenic 25.0%
!! TTGAATTCAACAACTCTTTT+TGG - chr2_4:20481474-20481493 None:intergenic 25.0%
!!! TTCTATTTTCTCTTTTTGAG+AGG - chr2_4:20481544-20481563 None:intergenic 25.0%
!!! TGAAGCTCTAAATTTATTGA+TGG - chr2_4:20481609-20481628 None:intergenic 25.0%
!!! AACTGTTGTTTATGGTATTT+TGG + chr2_4:20482196-20482215 Msa0320200:intron 25.0%
!!! ATGGTATTTTGGTATTTTTG+TGG + chr2_4:20482207-20482226 Msa0320200:intron 25.0%
!!! TTACACGGTTGATTTTTTTT+AGG + chr2_4:20482307-20482326 Msa0320200:intron 25.0%
! ATTTATGATGTGTGTATTGT+AGG + chr2_4:20482471-20482490 Msa0320200:intron 25.0%
! TTTCACTGAAATCTTAACAA+TGG - chr2_4:20482649-20482668 None:intergenic 25.0%
!! TAATTTTACAGCCTATGATA+TGG + chr2_4:20482819-20482838 Msa0320200:three_prime_UTR 25.0%
!!! TACTAATTTAGTTTGTGATG+CGG - chr2_4:20481124-20481143 None:intergenic 25.0%
! ATATGCAAGAAGTAAATGAA+AGG - chr2_4:20481168-20481187 None:intergenic 25.0%
! TTCAACAATTTCAACACTTT+GGG - chr2_4:20481331-20481350 None:intergenic 25.0%
!! TTGAATTCAACAACTCTTTT+TGG - chr2_4:20481474-20481493 None:intergenic 25.0%
!!! TTCTATTTTCTCTTTTTGAG+AGG - chr2_4:20481544-20481563 None:intergenic 25.0%
!!! TGAAGCTCTAAATTTATTGA+TGG - chr2_4:20481609-20481628 None:intergenic 25.0%
!!! AACTGTTGTTTATGGTATTT+TGG + chr2_4:20482196-20482215 Msa0320200:intron 25.0%
!!! ATGGTATTTTGGTATTTTTG+TGG + chr2_4:20482207-20482226 Msa0320200:intron 25.0%
!!! TTACACGGTTGATTTTTTTT+AGG + chr2_4:20482307-20482326 Msa0320200:intron 25.0%
! ATTTATGATGTGTGTATTGT+AGG + chr2_4:20482471-20482490 Msa0320200:intron 25.0%
! TTTCACTGAAATCTTAACAA+TGG - chr2_4:20482649-20482668 None:intergenic 25.0%
!! TAATTTTACAGCCTATGATA+TGG + chr2_4:20482819-20482838 Msa0320200:three_prime_UTR 25.0%
CTTCAACAATTTCAACACTT+TGG - chr2_4:20481332-20481351 None:intergenic 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG + chr2_4:20481330-20481349 Msa0320200:CDS 30.0%
GTTGAAATTGTTGAAGGAAA+TGG + chr2_4:20481336-20481355 Msa0320200:CDS 30.0%
AGACATGGATAATTTCTTGA+TGG - chr2_4:20481373-20481392 None:intergenic 30.0%
AAGAAATTATCCATGTCTGA+AGG + chr2_4:20481375-20481394 Msa0320200:CDS 30.0%
TAATCAATTACCTTCAGACA+TGG - chr2_4:20481388-20481407 None:intergenic 30.0%
!! CAAAGAACAACCATTTTTCA+TGG + chr2_4:20481627-20481646 Msa0320200:intron 30.0%
ATACAATTCATCCAAGGTTA+TGG + chr2_4:20481692-20481711 Msa0320200:intron 30.0%
! TTCTAGTTAGCACAAATAGT+TGG - chr2_4:20481810-20481829 None:intergenic 30.0%
ACTATTTGTGCTAACTAGAA+AGG + chr2_4:20481810-20481829 Msa0320200:intron 30.0%
TCAGTGAATCACTATTACAA+TGG - chr2_4:20481838-20481857 None:intergenic 30.0%
TGACTAATATATATCACGAG+AGG - chr2_4:20481866-20481885 None:intergenic 30.0%
AATCAGCCTAACATAATATG+TGG + chr2_4:20482030-20482049 Msa0320200:intron 30.0%
TGTAGACCACATATTATGTT+AGG - chr2_4:20482039-20482058 None:intergenic 30.0%
! TAATTTGTGTAGCACAAAGT+CGG - chr2_4:20482505-20482524 None:intergenic 30.0%
CTTCAACAATTTCAACACTT+TGG - chr2_4:20481332-20481351 None:intergenic 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG + chr2_4:20481330-20481349 Msa0320200:CDS 30.0%
GTTGAAATTGTTGAAGGAAA+TGG + chr2_4:20481336-20481355 Msa0320200:CDS 30.0%
AGACATGGATAATTTCTTGA+TGG - chr2_4:20481373-20481392 None:intergenic 30.0%
AAGAAATTATCCATGTCTGA+AGG + chr2_4:20481375-20481394 Msa0320200:CDS 30.0%
TAATCAATTACCTTCAGACA+TGG - chr2_4:20481388-20481407 None:intergenic 30.0%
!! CAAAGAACAACCATTTTTCA+TGG + chr2_4:20481627-20481646 Msa0320200:intron 30.0%
ATACAATTCATCCAAGGTTA+TGG + chr2_4:20481692-20481711 Msa0320200:intron 30.0%
! TTCTAGTTAGCACAAATAGT+TGG - chr2_4:20481810-20481829 None:intergenic 30.0%
ACTATTTGTGCTAACTAGAA+AGG + chr2_4:20481810-20481829 Msa0320200:intron 30.0%
TCAGTGAATCACTATTACAA+TGG - chr2_4:20481838-20481857 None:intergenic 30.0%
TGACTAATATATATCACGAG+AGG - chr2_4:20481866-20481885 None:intergenic 30.0%
AATCAGCCTAACATAATATG+TGG + chr2_4:20482030-20482049 Msa0320200:intron 30.0%
TGTAGACCACATATTATGTT+AGG - chr2_4:20482039-20482058 None:intergenic 30.0%
! TAATTTGTGTAGCACAAAGT+CGG - chr2_4:20482505-20482524 None:intergenic 30.0%
GAAATTGTTGAAGGAAATGG+AGG + chr2_4:20481339-20481358 Msa0320200:CDS 35.0%
! GATAATTTCTTGATGGTTCC+AGG - chr2_4:20481366-20481385 None:intergenic 35.0%
!! TTGTTGATTCTCCATAACCT+TGG - chr2_4:20481706-20481725 None:intergenic 35.0%
TGTGGTCTACAACTATAACT+TGG + chr2_4:20482048-20482067 Msa0320200:intron 35.0%
! CGACAGTTAACTGTTGTTTA+TGG + chr2_4:20482188-20482207 Msa0320200:intron 35.0%
! TTAGGACTAGAGTTGATTGT+TGG + chr2_4:20482325-20482344 Msa0320200:intron 35.0%
! AACGATATCCAGTTACTTTC+AGG + chr2_4:20482381-20482400 Msa0320200:intron 35.0%
TATGATGTGTGTATTGTAGG+TGG + chr2_4:20482474-20482493 Msa0320200:intron 35.0%
GGATACAACTATAGCATAGT+AGG + chr2_4:20482549-20482568 Msa0320200:CDS 35.0%
TACAACTATAGCATAGTAGG+AGG + chr2_4:20482552-20482571 Msa0320200:CDS 35.0%
! GGTCTATGTTGGATATTTTG+AGG + chr2_4:20482891-20482910 Msa0320200:three_prime_UTR 35.0%
!!! TTGAGGTTTGAGTGTTGTTT+TGG + chr2_4:20482908-20482927 Msa0320200:three_prime_UTR 35.0%
!!! TGAGGTTTGAGTGTTGTTTT+GGG + chr2_4:20482909-20482928 Msa0320200:three_prime_UTR 35.0%
GAAATTGTTGAAGGAAATGG+AGG + chr2_4:20481339-20481358 Msa0320200:CDS 35.0%
! GATAATTTCTTGATGGTTCC+AGG - chr2_4:20481366-20481385 None:intergenic 35.0%
!! TTGTTGATTCTCCATAACCT+TGG - chr2_4:20481706-20481725 None:intergenic 35.0%
TGTGGTCTACAACTATAACT+TGG + chr2_4:20482048-20482067 Msa0320200:intron 35.0%
! CGACAGTTAACTGTTGTTTA+TGG + chr2_4:20482188-20482207 Msa0320200:intron 35.0%
! TTAGGACTAGAGTTGATTGT+TGG + chr2_4:20482325-20482344 Msa0320200:intron 35.0%
! AACGATATCCAGTTACTTTC+AGG + chr2_4:20482381-20482400 Msa0320200:intron 35.0%
TATGATGTGTGTATTGTAGG+TGG + chr2_4:20482474-20482493 Msa0320200:intron 35.0%
GGATACAACTATAGCATAGT+AGG + chr2_4:20482549-20482568 Msa0320200:CDS 35.0%
TACAACTATAGCATAGTAGG+AGG + chr2_4:20482552-20482571 Msa0320200:CDS 35.0%
! GGTCTATGTTGGATATTTTG+AGG + chr2_4:20482891-20482910 Msa0320200:three_prime_UTR 35.0%
!!! TTGAGGTTTGAGTGTTGTTT+TGG + chr2_4:20482908-20482927 Msa0320200:three_prime_UTR 35.0%
!!! TGAGGTTTGAGTGTTGTTTT+GGG + chr2_4:20482909-20482928 Msa0320200:three_prime_UTR 35.0%
TGATGAACATGTCTCAACTG+TGG + chr2_4:20481236-20481255 Msa0320200:CDS 40.0%
! AGAGCTTTGTAGAGTTTAGC+TGG - chr2_4:20481264-20481283 None:intergenic 40.0%
TGCTGATGAAATCATCCCAA+AGG + chr2_4:20481293-20481312 Msa0320200:CDS 40.0%
GGGGATATACAATTCATCCA+AGG + chr2_4:20481686-20481705 Msa0320200:intron 40.0%
ATTTGTACTCAAATCGCTCC+AGG + chr2_4:20481771-20481790 Msa0320200:intron 40.0%
TTTGTACTCAAATCGCTCCA+GGG + chr2_4:20481772-20481791 Msa0320200:intron 40.0%
!!! GGGGTATTTTGGTATTTTCG+CGG - chr2_4:20482124-20482143 None:intergenic 40.0%
!!! TAACTATCGTGGGGGTATTT+TGG - chr2_4:20482135-20482154 None:intergenic 40.0%
GCTACACAAATTAGAAGCAG+TGG + chr2_4:20482512-20482531 Msa0320200:CDS 40.0%
CAAATTAGAAGCAGTGGATG+AGG + chr2_4:20482518-20482537 Msa0320200:CDS 40.0%
TATAGCATAGTAGGAGGAAC+AGG + chr2_4:20482558-20482577 Msa0320200:CDS 40.0%
ATAGCATAGTAGGAGGAACA+GGG + chr2_4:20482559-20482578 Msa0320200:CDS 40.0%
! TGAATTCGAGACAAGTGTTG+TGG + chr2_4:20482605-20482624 Msa0320200:CDS 40.0%
TCAGTGAAATACCACACCAA+AGG + chr2_4:20482660-20482679 Msa0320200:CDS 40.0%
AGATAGAACTGCATCACCTT+TGG - chr2_4:20482679-20482698 None:intergenic 40.0%
CTTGATAAGTCCAGTTCCTT+TGG - chr2_4:20482727-20482746 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG + chr2_4:20482725-20482744 Msa0320200:CDS 40.0%
TGATGAACATGTCTCAACTG+TGG + chr2_4:20481236-20481255 Msa0320200:CDS 40.0%
! AGAGCTTTGTAGAGTTTAGC+TGG - chr2_4:20481264-20481283 None:intergenic 40.0%
TGCTGATGAAATCATCCCAA+AGG + chr2_4:20481293-20481312 Msa0320200:CDS 40.0%
GGGGATATACAATTCATCCA+AGG + chr2_4:20481686-20481705 Msa0320200:intron 40.0%
ATTTGTACTCAAATCGCTCC+AGG + chr2_4:20481771-20481790 Msa0320200:intron 40.0%
TTTGTACTCAAATCGCTCCA+GGG + chr2_4:20481772-20481791 Msa0320200:intron 40.0%
!!! GGGGTATTTTGGTATTTTCG+CGG - chr2_4:20482124-20482143 None:intergenic 40.0%
!!! TAACTATCGTGGGGGTATTT+TGG - chr2_4:20482135-20482154 None:intergenic 40.0%
GCTACACAAATTAGAAGCAG+TGG + chr2_4:20482512-20482531 Msa0320200:CDS 40.0%
CAAATTAGAAGCAGTGGATG+AGG + chr2_4:20482518-20482537 Msa0320200:CDS 40.0%
TATAGCATAGTAGGAGGAAC+AGG + chr2_4:20482558-20482577 Msa0320200:CDS 40.0%
ATAGCATAGTAGGAGGAACA+GGG + chr2_4:20482559-20482578 Msa0320200:CDS 40.0%
! TGAATTCGAGACAAGTGTTG+TGG + chr2_4:20482605-20482624 Msa0320200:CDS 40.0%
TCAGTGAAATACCACACCAA+AGG + chr2_4:20482660-20482679 Msa0320200:CDS 40.0%
AGATAGAACTGCATCACCTT+TGG - chr2_4:20482679-20482698 None:intergenic 40.0%
CTTGATAAGTCCAGTTCCTT+TGG - chr2_4:20482727-20482746 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG + chr2_4:20482725-20482744 Msa0320200:CDS 40.0%
ATTTCAACACTTTGGGCAGC+TGG - chr2_4:20481324-20481343 None:intergenic 45.0%
GGTGTGTAGGAGCAAAAATG+AGG - chr2_4:20482103-20482122 None:intergenic 45.0%
! TTGGTATTTTCGCGGTGTGT+AGG - chr2_4:20482116-20482135 None:intergenic 45.0%
AGTGGCAGATAACTATCGTG+GGG - chr2_4:20482144-20482163 None:intergenic 45.0%
GAGTGGCAGATAACTATCGT+GGG - chr2_4:20482145-20482164 None:intergenic 45.0%
CACGATAGTTATCTGCCACT+CGG + chr2_4:20482144-20482163 Msa0320200:intron 45.0%
TCGGTTGACCTGAAAGTAAC+TGG - chr2_4:20482392-20482411 None:intergenic 45.0%
! TCTATCTGAAGCAGTACGTG+AGG + chr2_4:20482692-20482711 Msa0320200:CDS 45.0%
! AAGTCCAGTTCCTTTGGCTT+TGG - chr2_4:20482721-20482740 None:intergenic 45.0%
TGGACTTATCAAGGCCATTG+AGG + chr2_4:20482734-20482753 Msa0320200:CDS 45.0%
GGACTTATCAAGGCCATTGA+GGG + chr2_4:20482735-20482754 Msa0320200:CDS 45.0%
TGCCAAAACGTAACCCTCAA+TGG - chr2_4:20482751-20482770 None:intergenic 45.0%
! TCTAGCTCGTACATGTGTGT+GGG + chr2_4:20482870-20482889 Msa0320200:three_prime_UTR 45.0%
ACATGTGTGTGGGTCTATGT+TGG + chr2_4:20482880-20482899 Msa0320200:three_prime_UTR 45.0%
ATTTCAACACTTTGGGCAGC+TGG - chr2_4:20481324-20481343 None:intergenic 45.0%
GGTGTGTAGGAGCAAAAATG+AGG - chr2_4:20482103-20482122 None:intergenic 45.0%
! TTGGTATTTTCGCGGTGTGT+AGG - chr2_4:20482116-20482135 None:intergenic 45.0%
AGTGGCAGATAACTATCGTG+GGG - chr2_4:20482144-20482163 None:intergenic 45.0%
GAGTGGCAGATAACTATCGT+GGG - chr2_4:20482145-20482164 None:intergenic 45.0%
CACGATAGTTATCTGCCACT+CGG + chr2_4:20482144-20482163 Msa0320200:intron 45.0%
TCGGTTGACCTGAAAGTAAC+TGG - chr2_4:20482392-20482411 None:intergenic 45.0%
! TCTATCTGAAGCAGTACGTG+AGG + chr2_4:20482692-20482711 Msa0320200:CDS 45.0%
! AAGTCCAGTTCCTTTGGCTT+TGG - chr2_4:20482721-20482740 None:intergenic 45.0%
TGGACTTATCAAGGCCATTG+AGG + chr2_4:20482734-20482753 Msa0320200:CDS 45.0%
GGACTTATCAAGGCCATTGA+GGG + chr2_4:20482735-20482754 Msa0320200:CDS 45.0%
TGCCAAAACGTAACCCTCAA+TGG - chr2_4:20482751-20482770 None:intergenic 45.0%
! TCTAGCTCGTACATGTGTGT+GGG + chr2_4:20482870-20482889 Msa0320200:three_prime_UTR 45.0%
ACATGTGTGTGGGTCTATGT+TGG + chr2_4:20482880-20482899 Msa0320200:three_prime_UTR 45.0%
GTTGAAGGAAATGGAGGACC+TGG + chr2_4:20481345-20481364 Msa0320200:CDS 50.0%
TCAAATCGCTCCAGGGTAAG+CGG + chr2_4:20481779-20481798 Msa0320200:intron 50.0%
GTGGCAGATAACTATCGTGG+GGG - chr2_4:20482143-20482162 None:intergenic 50.0%
CGAGTGGCAGATAACTATCG+TGG - chr2_4:20482146-20482165 None:intergenic 50.0%
CCACAACAACATTTGCCGAG+TGG - chr2_4:20482162-20482181 None:intergenic 50.0%
CCACTCGGCAAATGTTGTTG+TGG + chr2_4:20482159-20482178 Msa0320200:intron 50.0%
AGCAGTGGATGAGGCAAACT+TGG + chr2_4:20482527-20482546 Msa0320200:CDS 50.0%
GCAGTGGATGAGGCAAACTT+GGG + chr2_4:20482528-20482547 Msa0320200:CDS 50.0%
CATAGTAGGAGGAACAGGGT+TGG + chr2_4:20482563-20482582 Msa0320200:CDS 50.0%
! TTCGAGACAAGTGTTGTGGC+TGG + chr2_4:20482609-20482628 Msa0320200:CDS 50.0%
GAACTGCATCACCTTTGGTG+TGG - chr2_4:20482674-20482693 None:intergenic 50.0%
GAGACCAAAGCCAAAGGAAC+TGG + chr2_4:20482714-20482733 Msa0320200:CDS 50.0%
! GGCCATTGAGGGTTACGTTT+TGG + chr2_4:20482746-20482765 Msa0320200:CDS 50.0%
! CTCTAGCTCGTACATGTGTG+TGG + chr2_4:20482869-20482888 Msa0320200:three_prime_UTR 50.0%
GTTGAAGGAAATGGAGGACC+TGG + chr2_4:20481345-20481364 Msa0320200:CDS 50.0%
TCAAATCGCTCCAGGGTAAG+CGG + chr2_4:20481779-20481798 Msa0320200:intron 50.0%
GTGGCAGATAACTATCGTGG+GGG - chr2_4:20482143-20482162 None:intergenic 50.0%
CGAGTGGCAGATAACTATCG+TGG - chr2_4:20482146-20482165 None:intergenic 50.0%
CCACAACAACATTTGCCGAG+TGG - chr2_4:20482162-20482181 None:intergenic 50.0%
CCACTCGGCAAATGTTGTTG+TGG + chr2_4:20482159-20482178 Msa0320200:intron 50.0%
AGCAGTGGATGAGGCAAACT+TGG + chr2_4:20482527-20482546 Msa0320200:CDS 50.0%
GCAGTGGATGAGGCAAACTT+GGG + chr2_4:20482528-20482547 Msa0320200:CDS 50.0%
CATAGTAGGAGGAACAGGGT+TGG + chr2_4:20482563-20482582 Msa0320200:CDS 50.0%
! TTCGAGACAAGTGTTGTGGC+TGG + chr2_4:20482609-20482628 Msa0320200:CDS 50.0%
GAACTGCATCACCTTTGGTG+TGG - chr2_4:20482674-20482693 None:intergenic 50.0%
GAGACCAAAGCCAAAGGAAC+TGG + chr2_4:20482714-20482733 Msa0320200:CDS 50.0%
! GGCCATTGAGGGTTACGTTT+TGG + chr2_4:20482746-20482765 Msa0320200:CDS 50.0%
! CTCTAGCTCGTACATGTGTG+TGG + chr2_4:20482869-20482888 Msa0320200:three_prime_UTR 50.0%
GGGCAGCTGGAATCACCTTT+GGG - chr2_4:20481311-20481330 None:intergenic 55.0%
TGGGCAGCTGGAATCACCTT+TGG - chr2_4:20481312-20481331 None:intergenic 55.0%
GTTGGATCTTCCGCTTACCC+TGG - chr2_4:20481792-20481811 None:intergenic 55.0%
!! AGTGTTGTGGCTGGCTCTGA+TGG + chr2_4:20482618-20482637 Msa0320200:CDS 55.0%
CGTGAGGAGACCAAAGCCAA+AGG + chr2_4:20482708-20482727 Msa0320200:CDS 55.0%
GGGCAGCTGGAATCACCTTT+GGG - chr2_4:20481311-20481330 None:intergenic 55.0%
TGGGCAGCTGGAATCACCTT+TGG - chr2_4:20481312-20481331 None:intergenic 55.0%
GTTGGATCTTCCGCTTACCC+TGG - chr2_4:20481792-20481811 None:intergenic 55.0%
!! AGTGTTGTGGCTGGCTCTGA+TGG + chr2_4:20482618-20482637 Msa0320200:CDS 55.0%
CGTGAGGAGACCAAAGCCAA+AGG + chr2_4:20482708-20482727 Msa0320200:CDS 55.0%
!! GTTGTGGCTGGCTCTGATGG+TGG + chr2_4:20482621-20482640 Msa0320200:CDS 60.0%
!! GTTGTGGCTGGCTCTGATGG+TGG + chr2_4:20482621-20482640 Msa0320200:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 20481121 20482935 20481121 ID=Msa0320200;Name=Msa0320200
chr2_4 mRNA 20481121 20482935 20481121 ID=Msa0320200-mRNA-1;Parent=Msa0320200;Name=Msa0320200-mRNA-1;_AED=0.38;_eAED=0.38;_QI=95|1|1|1|0.5|0.33|3|128|157
chr2_4 exon 20481121 20481396 20481121 ID=Msa0320200-mRNA-1:exon:7531;Parent=Msa0320200-mRNA-1
chr2_4 exon 20482493 20482791 20482493 ID=Msa0320200-mRNA-1:exon:7532;Parent=Msa0320200-mRNA-1
chr2_4 exon 20482814 20482935 20482814 ID=Msa0320200-mRNA-1:exon:7533;Parent=Msa0320200-mRNA-1
chr2_4 five_prime_UTR 20481121 20481215 20481121 ID=Msa0320200-mRNA-1:five_prime_utr;Parent=Msa0320200-mRNA-1
chr2_4 CDS 20481216 20481396 20481216 ID=Msa0320200-mRNA-1:cds;Parent=Msa0320200-mRNA-1
chr2_4 CDS 20482493 20482785 20482493 ID=Msa0320200-mRNA-1:cds;Parent=Msa0320200-mRNA-1
chr2_4 three_prime_UTR 20482786 20482791 20482786 ID=Msa0320200-mRNA-1:three_prime_utr;Parent=Msa0320200-mRNA-1
chr2_4 three_prime_UTR 20482814 20482935 20482814 ID=Msa0320200-mRNA-1:three_prime_utr;Parent=Msa0320200-mRNA-1
Gene Sequence

>Msa0320200

ATGGGTGTTTTTGTTTTCATTGATGAACATGTCTCAACTGTGGCTCCAGCTAAACTCTACAAAGCTCTTGCAAAAGATGCTGATGAAATCATCCCAAAGGTGATTCCAGCTGCCCAAAGTGTTGAAATTGTTGAAGGAAATGGAGGACCTGGAACCATCAAGAAATTATCCATGTCTGAAGGTGGCAAAACCGACTTTGTGCTACACAAATTAGAAGCAGTGGATGAGGCAAACTTGGGATACAACTATAGCATAGTAGGAGGAACAGGGTTGGATGAAAGTTTAGAGAAAGTTGAATTCGAGACAAGTGTTGTGGCTGGCTCTGATGGTGGTTCCATTGTTAAGATTTCAGTGAAATACCACACCAAAGGTGATGCAGTTCTATCTGAAGCAGTACGTGAGGAGACCAAAGCCAAAGGAACTGGACTTATCAAGGCCATTGAGGGTTACGTTTTGGCAAACCCTAATTATTAA
Protein sequence

>Msa0320200

MGVFVFIDEHVSTVAPAKLYKALAKDADEIIPKVIPAAQSVEIVEGNGGPGTIKKLSMSEGGKTDFVLHKLEAVDEANLGYNYSIVGGTGLDESLEKVEFETSVVAGSDGGSIVKISVKYHTKGDAVLSEAVREETKAKGTGLIKAIEGYVLANPNY*