AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0342410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0342410 MtrunA17_Chr2g0295124 96.907 97 3 0 2 98 61 157 3.29e-63 188
Msa0342410 MtrunA17_Chr2g0295104 93.814 97 6 0 2 98 61 157 3.39e-61 183
Msa0342410 MtrunA17_Chr2g0295091 92.708 96 7 0 3 98 62 157 1.25e-59 179
Msa0342410 MtrunA17_Chr2g0295141 86.458 96 13 0 3 98 62 157 1.04e-56 172
Msa0342410 MtrunA17_Chr6g0462311 65.979 97 33 0 2 98 61 157 2.44e-39 128
Msa0342410 MtrunA17_Chr4g0067951 65.979 97 33 0 2 98 61 157 4.31e-39 127
Msa0342410 MtrunA17_Chr2g0295064 61.458 96 37 0 3 98 129 224 6.09e-35 119
Msa0342410 MtrunA17_Chr2g0295021 58.763 97 40 0 2 98 61 157 7.47e-35 116
Msa0342410 MtrunA17_Chr2g0295001 58.763 97 40 0 2 98 61 157 7.47e-35 116
Msa0342410 MtrunA17_Chr2g0295051 57.292 96 41 0 3 98 62 157 5.80e-34 114
Msa0342410 MtrunA17_Chr2g0295041 58.333 96 40 0 3 98 62 157 1.52e-33 113
Msa0342410 MtrunA17_Chr2g0295081 52.577 97 46 0 2 98 61 157 2.29e-30 105
Msa0342410 MtrunA17_Chr6g0462291 62.500 80 30 0 2 81 61 140 2.45e-28 100
Msa0342410 MtrunA17_Chr4g0067921 40.659 91 52 2 10 98 68 158 1.05e-15 68.2
Msa0342410 MtrunA17_Chr2g0295211 31.111 90 62 0 7 96 67 156 7.60e-13 60.5
Msa0342410 MtrunA17_Chr4g0067841 29.167 96 68 0 2 97 62 157 3.39e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACATTTACTTAATAATT+AGG 0.132764 2_4:-65101904 None:intergenic
TTGAGGTTTGAGTGTTGTTT+TGG 0.265436 2_4:+65102038 Msa0342410:three_prime_UTR
GGCCATTGAGGGTTACGTTT+TGG 0.315356 2_4:+65101876 Msa0342410:CDS
CTTGATAAGTCCAGTTCCTT+TGG 0.329858 2_4:-65101854 None:intergenic
ATACATTTACTTAATAATTA+GGG 0.345110 2_4:-65101903 None:intergenic
AGCAGTGGATGAGGCAAACT+TGG 0.402684 2_4:+65101657 Msa0342410:CDS
TATAGCATAGTAGGAGGAAC+AGG 0.403927 2_4:+65101688 Msa0342410:CDS
AGATAGAACTGCATCACCTT+TGG 0.408990 2_4:-65101806 None:intergenic
GAGACTAAGGCCAAAGGAAC+TGG 0.415366 2_4:+65101844 Msa0342410:CDS
AGTGTTGTGGCTGGCTCTGA+TGG 0.429803 2_4:+65101748 Msa0342410:CDS
TTATTAAACAACCATATCAT+AGG 0.433028 2_4:-65101960 None:intergenic
TTATTAAGTAAATGTATACT+TGG 0.433767 2_4:+65101909 Msa0342410:exon
GGATACAACTATAGCATAGT+AGG 0.470749 2_4:+65101679 Msa0342410:CDS
GGACTTATCAAGGCCATTGA+GGG 0.495298 2_4:+65101865 Msa0342410:CDS
CTCTAGCTCGTACATGTGTG+TGG 0.498650 2_4:+65101999 Msa0342410:three_prime_UTR
TGCCAAAACGTAACCCTCAA+TGG 0.499044 2_4:-65101878 None:intergenic
CAAAGGAACTGGACTTATCA+AGG 0.516806 2_4:+65101855 Msa0342410:CDS
CAAATTAGAAGCAGTGGATG+AGG 0.520372 2_4:+65101648 Msa0342410:CDS
AGCAGTACGTGAGGAGACTA+AGG 0.536209 2_4:+65101831 Msa0342410:CDS
TGGACTTATCAAGGCCATTG+AGG 0.566375 2_4:+65101864 Msa0342410:CDS
TTTCACTGAAATCTTAACAA+TGG 0.569031 2_4:-65101776 None:intergenic
ACATGTGTGTGGGTCTATGT+TGG 0.584175 2_4:+65102010 Msa0342410:three_prime_UTR
TCTAGCTCGTACATGTGTGT+GGG 0.587397 2_4:+65102000 Msa0342410:three_prime_UTR
TAATTTGTGTAGCACAAAGT+CGG 0.587472 2_4:-65101632 None:intergenic
TTCGAGACAAGTGTTGTGGC+TGG 0.589865 2_4:+65101739 Msa0342410:CDS
GTTGTGGCTGGCTCTGATGG+TGG 0.598616 2_4:+65101751 Msa0342410:CDS
GCTACACAAATTAGAAGCAG+TGG 0.603203 2_4:+65101642 Msa0342410:CDS
CATAGTAGGAGGAACAGGGT+TGG 0.607807 2_4:+65101693 Msa0342410:CDS
GCAGTGGATGAGGCAAACTT+GGG 0.627060 2_4:+65101658 Msa0342410:CDS
TACAACTATAGCATAGTAGG+AGG 0.648903 2_4:+65101682 Msa0342410:CDS
TGAATTCGAGACAAGTGTTG+TGG 0.660471 2_4:+65101735 Msa0342410:CDS
TCAGTGAAATACCACACCAA+AGG 0.661287 2_4:+65101790 Msa0342410:CDS
CGTGAGGAGACTAAGGCCAA+AGG 0.670663 2_4:+65101838 Msa0342410:CDS
GAACTGCATCACCTTTGGTG+TGG 0.674778 2_4:-65101801 None:intergenic
TCTATCTGAAGCAGTACGTG+AGG 0.678507 2_4:+65101822 Msa0342410:CDS
ATAGCATAGTAGGAGGAACA+GGG 0.716365 2_4:+65101689 Msa0342410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACATTTACTTAATAATTA+GGG - chr2_4:65101906-65101925 None:intergenic 10.0%
!! TATACATTTACTTAATAATT+AGG - chr2_4:65101907-65101926 None:intergenic 10.0%
!! ATACATTTACTTAATAATTA+GGG - chr2_4:65101906-65101925 None:intergenic 10.0%
!! TATACATTTACTTAATAATT+AGG - chr2_4:65101907-65101926 None:intergenic 10.0%
!!! TCAAACTTTATTTACATTTA+AGG + chr2_4:65101561-65101580 Msa0342410:intron 15.0%
!! TTATTAAGTAAATGTATACT+TGG + chr2_4:65101909-65101928 Msa0342410:exon 15.0%
!!! TCAAACTTTATTTACATTTA+AGG + chr2_4:65101561-65101580 Msa0342410:intron 15.0%
!! TTATTAAGTAAATGTATACT+TGG + chr2_4:65101909-65101928 Msa0342410:exon 15.0%
!!! TTACACGATTGATTTTTTTT+AGG + chr2_4:65101437-65101456 Msa0342410:intron 20.0%
!! CTAAAAATTATTTCAGTCAT+CGG - chr2_4:65101541-65101560 None:intergenic 20.0%
!! TTATTAAACAACCATATCAT+AGG - chr2_4:65101963-65101982 None:intergenic 20.0%
!!! TTACACGATTGATTTTTTTT+AGG + chr2_4:65101437-65101456 Msa0342410:intron 20.0%
!! CTAAAAATTATTTCAGTCAT+CGG - chr2_4:65101541-65101560 None:intergenic 20.0%
!! TTATTAAACAACCATATCAT+AGG - chr2_4:65101963-65101982 None:intergenic 20.0%
!!! AACTGTTGTTTATGGTATTT+TGG + chr2_4:65101326-65101345 Msa0342410:intron 25.0%
!!! ATGGTATTTTGGTATTTTTG+TGG + chr2_4:65101337-65101356 Msa0342410:intron 25.0%
! ATTTATGATGTGTGTATTGT+AGG + chr2_4:65101601-65101620 Msa0342410:intron 25.0%
! TTTCACTGAAATCTTAACAA+TGG - chr2_4:65101779-65101798 None:intergenic 25.0%
!! TAATTTTACAGCCTATGATA+TGG + chr2_4:65101949-65101968 Msa0342410:three_prime_UTR 25.0%
!!! AACTGTTGTTTATGGTATTT+TGG + chr2_4:65101326-65101345 Msa0342410:intron 25.0%
!!! ATGGTATTTTGGTATTTTTG+TGG + chr2_4:65101337-65101356 Msa0342410:intron 25.0%
! ATTTATGATGTGTGTATTGT+AGG + chr2_4:65101601-65101620 Msa0342410:intron 25.0%
! TTTCACTGAAATCTTAACAA+TGG - chr2_4:65101779-65101798 None:intergenic 25.0%
!! TAATTTTACAGCCTATGATA+TGG + chr2_4:65101949-65101968 Msa0342410:three_prime_UTR 25.0%
! AATGTTAACTGTTGTTGTTG+TGG + chr2_4:65101289-65101308 Msa0342410:intron 30.0%
! TAATTTGTGTAGCACAAAGT+CGG - chr2_4:65101635-65101654 None:intergenic 30.0%
! AATGTTAACTGTTGTTGTTG+TGG + chr2_4:65101289-65101308 Msa0342410:intron 30.0%
! TAATTTGTGTAGCACAAAGT+CGG - chr2_4:65101635-65101654 None:intergenic 30.0%
! CGACAGTTAACTGTTGTTTA+TGG + chr2_4:65101318-65101337 Msa0342410:intron 35.0%
! TTAGGACTAGAGTTGATTGT+TGG + chr2_4:65101455-65101474 Msa0342410:intron 35.0%
! AACGATATCCAGTTACTTTC+AGG + chr2_4:65101511-65101530 Msa0342410:intron 35.0%
TATGATGTGTGTATTGTAGG+TGG + chr2_4:65101604-65101623 Msa0342410:intron 35.0%
GGATACAACTATAGCATAGT+AGG + chr2_4:65101679-65101698 Msa0342410:CDS 35.0%
TACAACTATAGCATAGTAGG+AGG + chr2_4:65101682-65101701 Msa0342410:CDS 35.0%
! GGTCTATGTTGGATATTTTG+AGG + chr2_4:65102021-65102040 Msa0342410:three_prime_UTR 35.0%
!!! TTGAGGTTTGAGTGTTGTTT+TGG + chr2_4:65102038-65102057 Msa0342410:three_prime_UTR 35.0%
!!! TGAGGTTTGAGTGTTGTTTT+GGG + chr2_4:65102039-65102058 Msa0342410:three_prime_UTR 35.0%
! CGACAGTTAACTGTTGTTTA+TGG + chr2_4:65101318-65101337 Msa0342410:intron 35.0%
! TTAGGACTAGAGTTGATTGT+TGG + chr2_4:65101455-65101474 Msa0342410:intron 35.0%
! AACGATATCCAGTTACTTTC+AGG + chr2_4:65101511-65101530 Msa0342410:intron 35.0%
TATGATGTGTGTATTGTAGG+TGG + chr2_4:65101604-65101623 Msa0342410:intron 35.0%
GGATACAACTATAGCATAGT+AGG + chr2_4:65101679-65101698 Msa0342410:CDS 35.0%
TACAACTATAGCATAGTAGG+AGG + chr2_4:65101682-65101701 Msa0342410:CDS 35.0%
! GGTCTATGTTGGATATTTTG+AGG + chr2_4:65102021-65102040 Msa0342410:three_prime_UTR 35.0%
!!! TTGAGGTTTGAGTGTTGTTT+TGG + chr2_4:65102038-65102057 Msa0342410:three_prime_UTR 35.0%
!!! TGAGGTTTGAGTGTTGTTTT+GGG + chr2_4:65102039-65102058 Msa0342410:three_prime_UTR 35.0%
CAGTTGAGACATGTTCATCA+AGG - chr2_4:65101236-65101255 None:intergenic 40.0%
TGATGAACATGTCTCAACTG+TGG + chr2_4:65101236-65101255 Msa0342410:intron 40.0%
! AGAGCTTTGTAGAGTTTAGC+TGG - chr2_4:65101264-65101283 None:intergenic 40.0%
GCTACACAAATTAGAAGCAG+TGG + chr2_4:65101642-65101661 Msa0342410:CDS 40.0%
CAAATTAGAAGCAGTGGATG+AGG + chr2_4:65101648-65101667 Msa0342410:CDS 40.0%
TATAGCATAGTAGGAGGAAC+AGG + chr2_4:65101688-65101707 Msa0342410:CDS 40.0%
ATAGCATAGTAGGAGGAACA+GGG + chr2_4:65101689-65101708 Msa0342410:CDS 40.0%
! TGAATTCGAGACAAGTGTTG+TGG + chr2_4:65101735-65101754 Msa0342410:CDS 40.0%
TCAGTGAAATACCACACCAA+AGG + chr2_4:65101790-65101809 Msa0342410:CDS 40.0%
AGATAGAACTGCATCACCTT+TGG - chr2_4:65101809-65101828 None:intergenic 40.0%
CTTGATAAGTCCAGTTCCTT+TGG - chr2_4:65101857-65101876 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG + chr2_4:65101855-65101874 Msa0342410:CDS 40.0%
CAGTTGAGACATGTTCATCA+AGG - chr2_4:65101236-65101255 None:intergenic 40.0%
TGATGAACATGTCTCAACTG+TGG + chr2_4:65101236-65101255 Msa0342410:intron 40.0%
! AGAGCTTTGTAGAGTTTAGC+TGG - chr2_4:65101264-65101283 None:intergenic 40.0%
GCTACACAAATTAGAAGCAG+TGG + chr2_4:65101642-65101661 Msa0342410:CDS 40.0%
CAAATTAGAAGCAGTGGATG+AGG + chr2_4:65101648-65101667 Msa0342410:CDS 40.0%
TATAGCATAGTAGGAGGAAC+AGG + chr2_4:65101688-65101707 Msa0342410:CDS 40.0%
ATAGCATAGTAGGAGGAACA+GGG + chr2_4:65101689-65101708 Msa0342410:CDS 40.0%
! TGAATTCGAGACAAGTGTTG+TGG + chr2_4:65101735-65101754 Msa0342410:CDS 40.0%
TCAGTGAAATACCACACCAA+AGG + chr2_4:65101790-65101809 Msa0342410:CDS 40.0%
AGATAGAACTGCATCACCTT+TGG - chr2_4:65101809-65101828 None:intergenic 40.0%
CTTGATAAGTCCAGTTCCTT+TGG - chr2_4:65101857-65101876 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG + chr2_4:65101855-65101874 Msa0342410:CDS 40.0%
TCGGTTGACCTGAAAGTAAC+TGG - chr2_4:65101522-65101541 None:intergenic 45.0%
! TCTATCTGAAGCAGTACGTG+AGG + chr2_4:65101822-65101841 Msa0342410:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG + chr2_4:65101864-65101883 Msa0342410:CDS 45.0%
GGACTTATCAAGGCCATTGA+GGG + chr2_4:65101865-65101884 Msa0342410:CDS 45.0%
TGCCAAAACGTAACCCTCAA+TGG - chr2_4:65101881-65101900 None:intergenic 45.0%
! TCTAGCTCGTACATGTGTGT+GGG + chr2_4:65102000-65102019 Msa0342410:three_prime_UTR 45.0%
ACATGTGTGTGGGTCTATGT+TGG + chr2_4:65102010-65102029 Msa0342410:three_prime_UTR 45.0%
TCGGTTGACCTGAAAGTAAC+TGG - chr2_4:65101522-65101541 None:intergenic 45.0%
! TCTATCTGAAGCAGTACGTG+AGG + chr2_4:65101822-65101841 Msa0342410:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG + chr2_4:65101864-65101883 Msa0342410:CDS 45.0%
GGACTTATCAAGGCCATTGA+GGG + chr2_4:65101865-65101884 Msa0342410:CDS 45.0%
TGCCAAAACGTAACCCTCAA+TGG - chr2_4:65101881-65101900 None:intergenic 45.0%
! TCTAGCTCGTACATGTGTGT+GGG + chr2_4:65102000-65102019 Msa0342410:three_prime_UTR 45.0%
ACATGTGTGTGGGTCTATGT+TGG + chr2_4:65102010-65102029 Msa0342410:three_prime_UTR 45.0%
AGCAGTGGATGAGGCAAACT+TGG + chr2_4:65101657-65101676 Msa0342410:CDS 50.0%
GCAGTGGATGAGGCAAACTT+GGG + chr2_4:65101658-65101677 Msa0342410:CDS 50.0%
CATAGTAGGAGGAACAGGGT+TGG + chr2_4:65101693-65101712 Msa0342410:CDS 50.0%
! TTCGAGACAAGTGTTGTGGC+TGG + chr2_4:65101739-65101758 Msa0342410:CDS 50.0%
GAACTGCATCACCTTTGGTG+TGG - chr2_4:65101804-65101823 None:intergenic 50.0%
AGCAGTACGTGAGGAGACTA+AGG + chr2_4:65101831-65101850 Msa0342410:CDS 50.0%
GAGACTAAGGCCAAAGGAAC+TGG + chr2_4:65101844-65101863 Msa0342410:CDS 50.0%
! GGCCATTGAGGGTTACGTTT+TGG + chr2_4:65101876-65101895 Msa0342410:CDS 50.0%
! CTCTAGCTCGTACATGTGTG+TGG + chr2_4:65101999-65102018 Msa0342410:three_prime_UTR 50.0%
AGCAGTGGATGAGGCAAACT+TGG + chr2_4:65101657-65101676 Msa0342410:CDS 50.0%
GCAGTGGATGAGGCAAACTT+GGG + chr2_4:65101658-65101677 Msa0342410:CDS 50.0%
CATAGTAGGAGGAACAGGGT+TGG + chr2_4:65101693-65101712 Msa0342410:CDS 50.0%
! TTCGAGACAAGTGTTGTGGC+TGG + chr2_4:65101739-65101758 Msa0342410:CDS 50.0%
GAACTGCATCACCTTTGGTG+TGG - chr2_4:65101804-65101823 None:intergenic 50.0%
AGCAGTACGTGAGGAGACTA+AGG + chr2_4:65101831-65101850 Msa0342410:CDS 50.0%
GAGACTAAGGCCAAAGGAAC+TGG + chr2_4:65101844-65101863 Msa0342410:CDS 50.0%
! GGCCATTGAGGGTTACGTTT+TGG + chr2_4:65101876-65101895 Msa0342410:CDS 50.0%
! CTCTAGCTCGTACATGTGTG+TGG + chr2_4:65101999-65102018 Msa0342410:three_prime_UTR 50.0%
!! AGTGTTGTGGCTGGCTCTGA+TGG + chr2_4:65101748-65101767 Msa0342410:CDS 55.0%
CGTGAGGAGACTAAGGCCAA+AGG + chr2_4:65101838-65101857 Msa0342410:CDS 55.0%
!! AGTGTTGTGGCTGGCTCTGA+TGG + chr2_4:65101748-65101767 Msa0342410:CDS 55.0%
CGTGAGGAGACTAAGGCCAA+AGG + chr2_4:65101838-65101857 Msa0342410:CDS 55.0%
!! GTTGTGGCTGGCTCTGATGG+TGG + chr2_4:65101751-65101770 Msa0342410:CDS 60.0%
!! GTTGTGGCTGGCTCTGATGG+TGG + chr2_4:65101751-65101770 Msa0342410:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 65101216 65102077 65101216 ID=Msa0342410;Name=Msa0342410
chr2_4 mRNA 65101216 65102077 65101216 ID=Msa0342410-mRNA-1;Parent=Msa0342410;Name=Msa0342410-mRNA-1;_AED=0.24;_eAED=0.25;_QI=0|0|0.5|1|1|1|2|162|98
chr2_4 exon 65101216 65101219 65101216 ID=Msa0342410-mRNA-1:exon:20767;Parent=Msa0342410-mRNA-1
chr2_4 exon 65101623 65102077 65101623 ID=Msa0342410-mRNA-1:exon:20768;Parent=Msa0342410-mRNA-1
chr2_4 CDS 65101216 65101219 65101216 ID=Msa0342410-mRNA-1:cds;Parent=Msa0342410-mRNA-1
chr2_4 CDS 65101623 65101915 65101623 ID=Msa0342410-mRNA-1:cds;Parent=Msa0342410-mRNA-1
chr2_4 three_prime_UTR 65101916 65102077 65101916 ID=Msa0342410-mRNA-1:three_prime_utr;Parent=Msa0342410-mRNA-1
Gene Sequence

>Msa0342410

ATGGGTGGCAAAACCGACTTTGTGCTACACAAATTAGAAGCAGTGGATGAGGCAAACTTGGGATACAACTATAGCATAGTAGGAGGAACAGGGTTGGATGAAAGTTTAGAGAAAGTTGAATTCGAGACAAGTGTTGTGGCTGGCTCTGATGGTGGTTCCATTGTTAAGATTTCAGTGAAATACCACACCAAAGGTGATGCAGTTCTATCTGAAGCAGTACGTGAGGAGACTAAGGCCAAAGGAACTGGACTTATCAAGGCCATTGAGGGTTACGTTTTGGCAAACCCTAATTATTAA

Protein sequence

>Msa0342410

MGGKTDFVLHKLEAVDEANLGYNYSIVGGTGLDESLEKVEFETSVVAGSDGGSIVKISVKYHTKGDAVLSEAVREETKAKGTGLIKAIEGYVLANPNY*