AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0320970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320970 MtrunA17_Chr2g0296621 87.195 328 14 3 12 312 17 343 0.0 575
Msa0320970 MtrunA17_Chr4g0076211 57.534 292 97 2 46 310 42 333 3.30e-119 346
Msa0320970 MtrunA17_Chr2g0292151 40.924 303 141 7 45 310 29 330 5.68e-64 206
Msa0320970 MtrunA17_Chr5g0402581 39.535 301 146 5 45 310 27 326 1.15e-63 204
Msa0320970 MtrunA17_Chr2g0292111 39.799 299 142 7 49 310 33 330 9.03e-63 202
Msa0320970 MtrunA17_Chr5g0402571 38.206 301 150 5 45 310 27 326 1.19e-62 201
Msa0320970 MtrunA17_Chr2g0291751 39.667 300 143 8 48 310 32 330 1.44e-61 199
Msa0320970 MtrunA17_Chr5g0404721 39.935 308 148 8 39 310 24 330 2.03e-61 198
Msa0320970 MtrunA17_Chr6g0460711 38.851 296 149 6 45 310 21 314 2.63e-61 197
Msa0320970 MtrunA17_Chr1g0194151 39.344 305 145 8 45 310 30 333 3.99e-60 195
Msa0320970 MtrunA17_Chr4g0046131 37.987 308 152 6 39 307 36 343 2.32e-59 193
Msa0320970 MtrunA17_Chr2g0292211 39.000 300 145 7 49 311 44 342 4.00e-59 193
Msa0320970 MtrunA17_Chr1g0188431 36.552 290 159 4 45 310 39 327 4.36e-59 192
Msa0320970 MtrunA17_Chr2g0292251 38.762 307 150 7 42 311 25 330 1.24e-58 192
Msa0320970 MtrunA17_Chr3g0106921 38.047 297 152 7 45 310 25 320 2.05e-58 190
Msa0320970 MtrunA17_Chr1g0194271 37.821 312 147 8 45 310 30 340 2.61e-58 191
Msa0320970 MtrunA17_Chr2g0279041 37.037 297 152 5 48 310 30 325 3.94e-58 189
Msa0320970 MtrunA17_Chr8g0372601 38.667 300 147 8 49 312 34 332 8.97e-58 189
Msa0320970 MtrunA17_Chr2g0292091 37.829 304 151 7 45 311 24 326 9.16e-58 189
Msa0320970 MtrunA17_Chr2g0292181 37.667 300 149 7 49 311 42 340 9.87e-58 189
Msa0320970 MtrunA17_Chr4g0038151 35.556 315 170 8 28 310 8 321 3.12e-57 187
Msa0320970 MtrunA17_Chr7g0254201 35.786 299 153 7 45 310 20 312 3.35e-57 187
Msa0320970 MtrunA17_Chr2g0320091 36.334 311 168 8 29 310 9 318 3.88e-57 187
Msa0320970 MtrunA17_Chr3g0114451 37.987 308 162 7 31 310 12 318 7.55e-57 186
Msa0320970 MtrunA17_Chr4g0038161 36.877 301 157 8 42 310 21 320 1.15e-56 186
Msa0320970 MtrunA17_Chr2g0292101 37.500 304 152 6 45 311 25 327 1.20e-56 186
Msa0320970 MtrunA17_Chr2g0322861 38.538 301 149 8 45 310 87 386 1.47e-56 187
Msa0320970 MtrunA17_Chr5g0432441 36.242 298 157 7 45 310 30 326 1.78e-56 185
Msa0320970 MtrunA17_Chr2g0292241 38.333 300 147 7 49 311 33 331 2.47e-56 186
Msa0320970 MtrunA17_Chr1g0164891 38.411 302 146 8 48 310 92 392 6.58e-56 186
Msa0320970 MtrunA17_Chr4g0063671 37.874 301 151 5 45 310 21 320 9.00e-56 183
Msa0320970 MtrunA17_Chr2g0292261 36.709 316 158 8 33 311 21 331 1.25e-55 184
Msa0320970 MtrunA17_Chr3g0128061 37.500 304 151 8 45 311 26 327 1.61e-55 183
Msa0320970 MtrunA17_Chr8g0371381 36.842 304 148 6 43 310 24 319 2.59e-55 182
Msa0320970 MtrunA17_Chr1g0181431 37.793 299 153 7 45 311 27 324 4.56e-55 182
Msa0320970 MtrunA17_Chr7g0268501 38.514 296 144 8 49 310 29 320 5.62e-55 181
Msa0320970 MtrunA17_Chr7g0268521 38.514 296 144 8 49 310 29 320 5.62e-55 181
Msa0320970 MtrunA17_Chr2g0320101 34.951 309 172 7 30 310 10 317 5.77e-55 181
Msa0320970 MtrunA17_Chr5g0400331 39.871 311 139 8 44 310 25 331 6.31e-55 181
Msa0320970 MtrunA17_Chr2g0320071 36.207 290 156 7 49 310 29 317 6.50e-55 181
Msa0320970 MtrunA17_Chr4g0024121 37.667 300 147 8 48 310 30 326 8.09e-55 181
Msa0320970 MtrunA17_Chr1g0175561 35.548 301 155 7 49 311 28 327 8.62e-55 181
Msa0320970 MtrunA17_Chr1g0181511 37.793 299 153 7 45 311 27 324 1.13e-54 181
Msa0320970 MtrunA17_Chr1g0157051 37.049 305 153 6 39 309 18 317 1.43e-54 180
Msa0320970 MtrunA17_Chr4g0013201 38.095 294 153 7 45 310 46 338 1.55e-54 181
Msa0320970 MtrunA17_Chr4g0043391 35.430 302 161 8 42 310 24 324 1.59e-54 180
Msa0320970 MtrunA17_Chr7g0254231 35.811 296 156 7 45 311 22 312 2.41e-54 179
Msa0320970 MtrunA17_Chr4g0038171 35.099 302 162 8 42 310 21 321 3.42e-54 179
Msa0320970 MtrunA17_Chr4g0013191 39.041 292 147 7 49 310 50 340 7.46e-54 179
Msa0320970 MtrunA17_Chr4g0072091 34.343 297 159 6 45 306 24 319 1.21e-53 178
Msa0320970 MtrunA17_Chr6g0467251 36.207 290 156 6 45 306 36 324 1.30e-53 178
Msa0320970 MtrunA17_Chr2g0292271 36.667 300 152 7 49 311 33 331 1.41e-53 179
Msa0320970 MtrunA17_Chr4g0013171 38.851 296 150 8 45 310 27 321 3.72e-53 176
Msa0320970 MtrunA17_Chr6g0467161 33.447 293 167 4 45 310 45 336 4.88e-53 177
Msa0320970 MtrunA17_Chr4g0068621 35.569 343 176 10 2 310 5 336 5.10e-53 177
Msa0320970 MtrunA17_Chr3g0129051 35.390 308 168 7 29 306 12 318 1.57e-52 175
Msa0320970 MtrunA17_Chr1g0213061 35.690 297 160 7 45 311 27 322 1.79e-52 175
Msa0320970 MtrunA17_Chr7g0258811 35.548 301 155 8 49 311 73 372 1.89e-52 176
Msa0320970 MtrunA17_Chr8g0364291 35.667 300 153 7 49 310 27 324 2.70e-52 174
Msa0320970 MtrunA17_Chr7g0224371 36.897 290 155 7 49 311 35 323 3.02e-52 174
Msa0320970 MtrunA17_Chr7g0244461 34.354 294 164 7 45 310 20 312 3.66e-52 174
Msa0320970 MtrunA17_Chr5g0401431 34.653 303 159 7 45 309 27 328 7.74e-52 173
Msa0320970 MtrunA17_Chr2g0320031 35.932 295 159 9 45 310 26 319 9.91e-52 173
Msa0320970 MtrunA17_Chr1g0181481 36.455 299 157 7 45 311 27 324 1.05e-51 173
Msa0320970 MtrunA17_Chr6g0467121 34.932 292 164 4 45 311 74 364 1.44e-51 174
Msa0320970 MtrunA17_Chr2g0320051 35.616 292 156 9 49 310 29 318 1.74e-51 172
Msa0320970 MtrunA17_Chr6g0477071 34.021 291 165 6 45 310 54 342 3.69e-51 172
Msa0320970 MtrunA17_Chr2g0292291 36.333 300 152 7 49 310 34 332 4.65e-51 172
Msa0320970 MtrunA17_Chr5g0405991 36.120 299 153 6 45 310 23 316 5.28e-51 171
Msa0320970 MtrunA17_Chr1g0148021 33.993 303 162 8 45 310 27 328 7.21e-51 171
Msa0320970 MtrunA17_Chr7g0244451 35.034 294 162 7 45 310 24 316 7.68e-51 170
Msa0320970 MtrunA17_Chr5g0438021 37.500 296 154 8 45 310 31 325 8.62e-51 171
Msa0320970 MtrunA17_Chr6g0451571 33.775 302 163 7 45 310 34 334 8.95e-51 171
Msa0320970 MtrunA17_Chr4g0013821 36.455 299 151 7 48 311 31 325 1.08e-50 171
Msa0320970 MtrunA17_Chr3g0129061 36.986 292 153 7 45 306 28 318 2.37e-50 169
Msa0320970 MtrunA17_Chr4g0070951 35.526 304 158 7 45 311 28 330 6.00e-50 168
Msa0320970 MtrunA17_Chr1g0155611 37.459 307 150 10 45 310 42 347 6.88e-50 169
Msa0320970 MtrunA17_Chr3g0106901 38.435 294 152 7 45 310 31 323 1.71e-49 167
Msa0320970 MtrunA17_Chr1g0197411 32.895 304 165 6 45 310 28 330 3.73e-49 166
Msa0320970 MtrunA17_Chr4g0053871 33.220 295 163 6 45 306 27 320 5.53e-49 166
Msa0320970 MtrunA17_Chr1g0204011 34.899 298 152 6 49 310 31 322 5.69e-49 166
Msa0320970 MtrunA17_Chr2g0320061 33.226 310 177 8 30 310 10 318 8.21e-49 165
Msa0320970 MtrunA17_Chr4g0013181 37.671 292 151 8 49 310 63 353 8.61e-48 163
Msa0320970 MtrunA17_Chr4g0013721 35.235 298 154 8 48 310 103 396 2.10e-47 164
Msa0320970 MtrunA17_Chr2g0299691 35.314 303 153 8 49 311 60 359 7.04e-47 161
Msa0320970 MtrunA17_Chr5g0432331 38.255 298 151 10 45 310 56 352 1.84e-46 160
Msa0320970 MtrunA17_Chr2g0278671 35.880 301 157 7 45 310 24 323 2.68e-46 159
Msa0320970 MtrunA17_Chr4g0074851 33.223 301 168 6 42 310 29 328 4.62e-46 158
Msa0320970 MtrunA17_Chr3g0107881 37.627 295 150 11 49 310 31 324 2.58e-45 156
Msa0320970 MtrunA17_Chr3g0129041 36.486 296 157 8 45 310 28 322 5.21e-45 155
Msa0320970 MtrunA17_Chr2g0316481 32.012 328 156 8 45 306 76 402 4.90e-44 155
Msa0320970 MtrunA17_Chr5g0432351 37.248 298 154 10 45 310 25 321 5.16e-44 153
Msa0320970 MtrunA17_Chr2g0316791 33.435 329 152 10 45 307 76 403 1.52e-43 154
Msa0320970 MtrunA17_Chr5g0432341 37.248 298 154 10 45 310 25 321 1.54e-43 152
Msa0320970 MtrunA17_Chr2g0316191 32.424 330 155 9 45 307 76 404 1.83e-43 154
Msa0320970 MtrunA17_Chr2g0316831 31.707 328 157 8 45 306 112 438 1.85e-43 154
Msa0320970 MtrunA17_Chr7g0225221 32.765 293 165 6 49 310 29 320 2.76e-43 151
Msa0320970 MtrunA17_Chr5g0421411 36.364 275 134 7 76 310 12 285 4.04e-43 149
Msa0320970 MtrunA17_Chr5g0413051 32.573 307 164 7 45 310 73 377 5.44e-43 151
Msa0320970 MtrunA17_Chr4g0075241 30.132 302 176 5 43 310 27 327 2.30e-42 149
Msa0320970 MtrunA17_Chr2g0279061 35.156 256 131 5 89 310 12 266 2.00e-40 142
Msa0320970 MtrunA17_Chr2g0311251 30.631 333 159 10 45 309 11 339 2.12e-40 144
Msa0320970 MtrunA17_Chr2g0329641 33.003 303 163 7 45 310 36 335 6.51e-37 134
Msa0320970 MtrunA17_Chr2g0311241 28.032 371 160 9 45 309 60 429 7.97e-37 136
Msa0320970 MtrunA17_Chr3g0136751 32.776 299 167 6 45 310 25 322 1.20e-35 131
Msa0320970 MtrunA17_Chr5g0432401 34.043 282 146 10 44 309 28 285 6.63e-34 125
Msa0320970 MtrunA17_Chr2g0316811 32.735 223 102 4 45 219 50 272 2.05e-32 122
Msa0320970 MtrunA17_Chr2g0292281 36.715 207 99 5 128 310 18 216 1.83e-31 117
Msa0320970 MtrunA17_Chr4g0004591 28.296 311 147 6 44 309 76 355 7.78e-30 116
Msa0320970 MtrunA17_Chr2g0316141 30.827 266 134 9 91 307 11 275 2.79e-29 113
Msa0320970 MtrunA17_Chr2g0316181 30.827 266 134 9 91 307 11 275 2.79e-29 113
Msa0320970 MtrunA17_Chr3g0114491 41.732 127 64 2 29 145 2 128 5.02e-26 100
Msa0320970 MtrunA17_Chr1g0181501 34.031 191 103 5 143 311 1 190 2.34e-22 92.4
Msa0320970 MtrunA17_Chr3g0114461 34.595 185 101 6 145 310 9 192 1.40e-19 84.7
Msa0320970 MtrunA17_Chr1g0181491 42.453 106 51 4 45 141 12 116 6.20e-15 70.9
Msa0320970 MtrunA17_Chr6g0468731 45.455 66 30 1 84 143 22 87 1.46e-13 65.5
Msa0320970 MtrunA17_Chr7g0254171 40.449 89 44 2 90 169 3 91 1.03e-12 64.7
Msa0320970 MtrunA17_Chr7g0254181 38.235 102 55 3 104 199 22 121 1.30e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320970 AT1G30870.1 54.000 300 109 4 41 311 47 346 1.68e-107 317
Msa0320970 AT5G17820.1 36.860 293 155 5 45 310 24 313 2.45e-67 213
Msa0320970 AT3G03670.1 37.667 300 152 6 45 310 23 321 5.73e-67 213
Msa0320970 AT5G17820.2 36.860 293 155 5 45 310 77 366 9.30e-66 211
Msa0320970 AT1G49570.1 39.073 302 145 7 45 308 48 348 1.99e-62 202
Msa0320970 AT1G05240.1 38.944 303 147 9 44 310 25 325 6.60e-62 199
Msa0320970 AT1G05250.1 38.944 303 147 9 44 310 25 325 6.60e-62 199
Msa0320970 AT4G36430.1 39.000 300 146 9 48 311 33 331 1.11e-61 199
Msa0320970 AT5G64120.1 37.543 293 153 5 47 310 37 328 1.79e-61 199
Msa0320970 AT2G18150.1 38.127 299 148 8 48 310 39 336 2.34e-61 199
Msa0320970 AT5G15180.1 41.472 299 141 8 45 310 32 329 2.89e-60 196
Msa0320970 AT3G01190.1 41.137 299 140 9 45 310 26 321 2.19e-59 193
Msa0320970 AT4G16270.1 39.869 306 145 8 41 310 46 348 6.99e-59 192
Msa0320970 AT4G11290.1 39.672 305 142 9 45 310 25 326 7.04e-59 192
Msa0320970 AT2G18140.1 37.898 314 149 9 42 310 23 335 1.66e-58 191
Msa0320970 AT1G05260.1 38.411 302 150 8 45 311 26 326 6.49e-58 189
Msa0320970 AT3G21770.1 38.721 297 146 8 45 306 29 324 7.04e-58 189
Msa0320970 AT2G38390.1 39.000 300 144 8 49 310 35 333 9.20e-58 189
Msa0320970 AT2G41480.1 37.624 303 153 5 43 310 27 328 1.58e-57 188
Msa0320970 AT4G08770.1 38.562 306 148 9 45 311 24 328 2.91e-57 188
Msa0320970 AT2G34060.1 40.066 302 142 7 45 307 42 343 4.33e-57 188
Msa0320970 AT2G39040.1 38.562 306 147 8 45 310 46 350 4.76e-57 188
Msa0320970 AT5G66390.1 38.591 298 146 8 49 310 37 333 5.74e-57 187
Msa0320970 AT4G33420.1 37.586 290 156 4 45 310 37 325 1.19e-56 186
Msa0320970 AT4G33420.2 37.586 290 156 4 45 310 84 372 3.18e-56 186
Msa0320970 AT4G08780.1 38.235 306 149 9 45 311 24 328 4.01e-56 185
Msa0320970 AT1G71695.1 35.873 315 162 8 34 310 27 339 8.22e-56 185
Msa0320970 AT5G64100.1 37.075 294 152 7 48 310 40 331 1.69e-55 183
Msa0320970 AT4G26010.1 36.207 290 159 6 45 310 22 309 2.14e-55 182
Msa0320970 AT4G26010.2 36.207 290 159 6 45 310 50 337 4.20e-55 182
Msa0320970 AT3G50990.1 37.086 302 153 8 45 310 41 341 6.21e-55 182
Msa0320970 AT5G05340.1 35.135 296 161 7 45 310 30 324 4.08e-54 179
Msa0320970 AT2G38380.1 38.667 300 145 8 49 310 35 333 4.54e-54 180
Msa0320970 AT3G49120.1 36.424 302 152 8 49 311 36 336 7.22e-54 180
Msa0320970 AT3G28200.1 35.017 297 161 6 45 310 21 316 7.85e-54 178
Msa0320970 AT3G32980.1 38.079 302 147 8 49 311 35 335 1.07e-53 179
Msa0320970 AT5G39580.1 35.034 294 160 4 47 310 27 319 1.41e-53 178
Msa0320970 AT4G17690.1 34.694 294 159 6 49 310 30 322 1.82e-53 178
Msa0320970 AT3G49110.1 36.570 309 156 9 42 311 30 337 1.93e-53 179
Msa0320970 AT5G19890.1 36.545 301 153 6 45 310 30 327 1.94e-53 178
Msa0320970 AT5G06730.1 34.667 300 158 8 49 311 37 335 2.65e-53 178
Msa0320970 AT5G40150.1 35.570 298 159 7 45 310 32 328 3.69e-53 177
Msa0320970 AT5G06720.1 34.333 300 159 7 49 311 36 334 1.37e-52 176
Msa0320970 AT5G19880.1 34.641 306 159 7 45 310 25 329 1.84e-52 175
Msa0320970 AT1G14550.1 38.796 299 147 9 45 310 26 321 2.02e-52 175
Msa0320970 AT1G68850.1 36.601 306 154 8 45 310 30 335 4.28e-52 174
Msa0320970 AT1G77100.1 35.354 297 160 7 45 310 24 319 5.76e-52 174
Msa0320970 AT2G24800.1 38.333 300 150 6 45 310 31 329 6.03e-52 174
Msa0320970 AT4G30170.1 32.558 301 166 7 45 310 27 325 6.59e-52 174
Msa0320970 AT5G64110.1 36.700 297 151 7 48 310 37 330 7.74e-52 174
Msa0320970 AT2G35380.1 36.842 304 150 10 48 310 33 335 1.62e-51 173
Msa0320970 AT5G42180.1 35.548 301 151 6 45 309 23 316 2.77e-51 172
Msa0320970 AT2G18980.1 34.219 301 161 7 45 310 25 323 3.28e-51 172
Msa0320970 AT5G47000.1 34.106 302 161 7 44 310 32 330 1.32e-50 171
Msa0320970 AT5G51890.1 35.333 300 149 7 49 310 30 322 1.42e-50 170
Msa0320970 AT3G49960.1 33.224 304 164 6 45 310 27 329 1.72e-50 170
Msa0320970 AT1G44970.1 37.124 299 150 8 49 310 49 346 3.55e-50 170
Msa0320970 AT2G37130.1 35.786 299 158 8 45 310 30 327 2.25e-49 167
Msa0320970 AT2G37130.2 35.690 297 157 8 47 310 1 296 3.55e-49 166
Msa0320970 AT1G34510.1 36.552 290 158 6 45 310 22 309 4.06e-49 166
Msa0320970 AT2G22420.1 36.027 297 151 8 49 310 27 319 5.68e-49 166
Msa0320970 AT4G31760.1 35.452 299 159 6 45 310 29 326 6.11e-49 166
Msa0320970 AT1G14540.1 35.811 296 159 9 45 310 21 315 2.83e-48 164
Msa0320970 AT5G14130.1 34.000 300 163 7 45 310 32 330 4.04e-48 164
Msa0320970 AT4G25980.1 35.762 302 157 6 45 310 26 326 5.78e-48 164
Msa0320970 AT5G14130.2 34.000 300 163 7 45 310 73 371 2.28e-47 163
Msa0320970 AT4G37520.1 31.908 304 168 7 45 310 27 329 4.36e-47 161
Msa0320970 AT4G37520.2 31.908 304 165 8 45 310 27 326 5.13e-46 158
Msa0320970 AT5G58390.1 34.602 289 157 7 49 306 25 312 5.17e-46 158
Msa0320970 AT4G37530.1 31.000 300 168 6 49 310 31 329 1.48e-45 157
Msa0320970 AT5G58400.1 32.602 319 182 8 20 306 4 321 2.59e-45 157
Msa0320970 AT5G22410.1 32.770 296 163 7 45 306 28 321 2.50e-44 154
Msa0320970 AT5G67400.1 30.938 320 181 7 29 310 12 329 2.62e-44 154
Msa0320970 AT4G21960.1 33.224 304 170 6 39 310 25 327 3.01e-44 154
Msa0320970 AT5G24070.1 32.566 304 166 6 45 311 35 336 1.48e-43 152
Msa0320970 AT1G24110.1 35.738 305 157 8 45 312 22 324 1.10e-42 150
Msa0320970 AT2G43480.1 30.960 323 178 7 25 310 21 335 2.66e-41 146
Msa0320970 AT3G17070.1 32.566 304 165 8 45 310 38 339 1.59e-40 144
Msa0320970 AT4G33870.1 27.671 365 187 9 12 307 42 398 2.08e-40 145
Msa0320970 AT2G24800.2 44.172 163 84 2 45 200 31 193 1.13e-37 134
Msa0320970 AT4G25980.2 45.679 162 81 2 45 199 71 232 7.24e-37 133
Msa0320970 AT4G37530.2 30.159 252 138 5 49 262 31 282 2.38e-36 132
Msa0320970 AT5G39580.2 38.365 159 92 1 47 199 27 185 5.32e-33 122
Msa0320970 AT2G35380.2 34.599 237 117 8 111 310 12 247 3.41e-32 120
Msa0320970 AT3G17070.2 29.845 258 141 8 91 310 21 276 1.28e-23 98.2

Find 56 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAGAAGTTAAGGTATTT+TGG 0.131323 2_4:-21958163 None:intergenic
ACTTCCATGATTGCTCTGTT+AGG 0.266056 2_4:+21956972 Msa0320970:CDS
CCATATTGGTCAGTCCCTTA+TGG 0.281860 2_4:+21957752 Msa0320970:CDS
GGTGTCATTGATGTTCTTAC+TGG 0.290141 2_4:+21958403 Msa0320970:CDS
AACATTAGAGGATACAGATT+TGG 0.336587 2_4:+21956826 Msa0320970:CDS
TCTTGCAAAGAAAATGTAGA+TGG 0.363928 2_4:+21958179 Msa0320970:CDS
TTGGATAATCTCTTATCCTT+TGG 0.369990 2_4:+21956845 Msa0320970:CDS
TCTACAAGTCCCAACATTAG+AGG 0.388107 2_4:+21956814 Msa0320970:CDS
GCTGTTTCAATGTCAAAGTT+TGG 0.390316 2_4:+21958382 Msa0320970:CDS
TTTCCATCTTTCCTCCCATA+AGG 0.401746 2_4:-21957767 None:intergenic
TTCCATCTTTCCTCCCATAA+GGG 0.402901 2_4:-21957766 None:intergenic
TTTGGATCTAATGATGGGTC+TGG 0.404975 2_4:-21958145 None:intergenic
CTTGACTTAGTTGTTCTCTC+AGG 0.406767 2_4:+21957884 Msa0320970:CDS
CATATTGGTCAGTCCCTTAT+GGG 0.409021 2_4:+21957753 Msa0320970:CDS
AGCATTGACAAAATTGCAAT+TGG 0.413153 2_4:-21958449 None:intergenic
GTCAAGGACAGTCAAGCCTT+TGG 0.419581 2_4:-21957867 None:intergenic
TATTGACAAAGAAGCTGAAA+TGG 0.421889 2_4:+21957796 Msa0320970:CDS
ATATTCTTACAACTGCTTCA+AGG 0.425738 2_4:+21957705 Msa0320970:CDS
GAAACAAGTGGAGAAGTTCT+TGG 0.427170 2_4:-21958319 None:intergenic
CTTCCATGATTGCTCTGTTA+GGG 0.430268 2_4:+21956973 Msa0320970:CDS
CATGTCATTTGTGTTCTACT+AGG 0.438974 2_4:+21956485 Msa0320970:CDS
CCATAAGGGACTGACCAATA+TGG 0.445922 2_4:-21957752 None:intergenic
TTCTCCACTTGTTTCAGCAT+TGG 0.449242 2_4:+21958327 Msa0320970:CDS
CCACCCTAACAGAGCAATCA+TGG 0.454699 2_4:-21956976 None:intergenic
AGGGATGCCACTGTTGAGTT+GGG 0.457543 2_4:+21957725 Msa0320970:CDS
GTCCCTTATGGGAGGAAAGA+TGG 0.458270 2_4:+21957764 Msa0320970:CDS
AAATCTGTATCCTCTAATGT+TGG 0.458659 2_4:-21956824 None:intergenic
TTTGACTTTGCTATGCAAGA+TGG 0.467791 2_4:-21956900 None:intergenic
TGCATAGCAAAGTCAAAGAA+TGG 0.476889 2_4:+21956906 Msa0320970:CDS
AAGGGATGCCACTGTTGAGT+TGG 0.488094 2_4:+21957724 Msa0320970:CDS
AATCTGTATCCTCTAATGTT+GGG 0.489759 2_4:-21956823 None:intergenic
TGCAAGATGGATTCAAATTG+AGG 0.491467 2_4:-21956887 None:intergenic
TTGAGTTGGGTGGACCATAT+TGG 0.502726 2_4:+21957738 Msa0320970:CDS
AATCATGGAAGTGCAACCTT+AGG 0.505171 2_4:-21956961 None:intergenic
TATTCTTACAACTGCTTCAA+GGG 0.505713 2_4:+21957706 Msa0320970:CDS
CTTTAGCAGCTAGTCTCCTA+AGG 0.506408 2_4:+21956945 Msa0320970:CDS
AGAAGCTGAAATGGTACCAA+TGG 0.515929 2_4:+21957805 Msa0320970:CDS
GGATTGAAAATATTCGATCA+AGG 0.518413 2_4:-21957846 None:intergenic
GAGATGATGAAGGAGAAATC+AGG 0.533880 2_4:+21958425 Msa0320970:CDS
GAAGCTGAAATGGTACCAAT+GGG 0.535263 2_4:+21957806 Msa0320970:CDS
GCAAGATGGATTCAAATTGA+GGG 0.538287 2_4:-21956886 None:intergenic
ACCTTATGATGAAAACTATG+AGG 0.539825 2_4:+21956778 Msa0320970:CDS
TAGATGGGCCACAGAATATG+TGG 0.542434 2_4:+21958195 Msa0320970:CDS
AGTCAAAGAATGGATTAAGA+AGG 0.547241 2_4:+21956916 Msa0320970:CDS
GTAATGTTCTCATGGCCCAT+TGG 0.578787 2_4:-21957821 None:intergenic
TCAAGGAAGTAATGTTCTCA+TGG 0.582299 2_4:-21957829 None:intergenic
CTTGCAAAGAAAATGTAGAT+GGG 0.598148 2_4:+21958180 Msa0320970:CDS
CAAGTAAGCAAGACATTGAG+AGG 0.601262 2_4:+21957614 Msa0320970:CDS
TGAATCATGAAGGAAGTGAA+AGG 0.602756 2_4:+21957585 Msa0320970:CDS
GTTCTTACTGGAGATGATGA+AGG 0.609230 2_4:+21958415 Msa0320970:CDS
CTGAGAGAACAACTAAGTCA+AGG 0.638471 2_4:-21957883 None:intergenic
ATCAAGATCCACATATTCTG+TGG 0.652227 2_4:-21958203 None:intergenic
GATGCCACTGTTGAGTTGGG+TGG 0.668142 2_4:+21957728 Msa0320970:CDS
GTGGCCAATGCTGAAACAAG+TGG 0.669223 2_4:-21958331 None:intergenic
ATTGGTCAGTCCCTTATGGG+AGG 0.681509 2_4:+21957756 Msa0320970:CDS
TGGTCCACCCAACTCAACAG+TGG 0.695362 2_4:-21957732 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATAGTAAAAAATGAATA+TGG - chr2_4:21957511-21957530 None:intergenic 10.0%
!! ATATTAATATAGAAAACAAT+TGG - chr2_4:21958047-21958066 None:intergenic 10.0%
!! TATATAGTAAAAAATGAATA+TGG - chr2_4:21957511-21957530 None:intergenic 10.0%
!! ATATTAATATAGAAAACAAT+TGG - chr2_4:21958047-21958066 None:intergenic 10.0%
!!! TGATTTATATAGTTTTAGTA+AGG - chr2_4:21956595-21956614 None:intergenic 15.0%
!!! ATATACTTATTTGAACTTTT+TGG + chr2_4:21957526-21957545 Msa0320970:intron 15.0%
!!! TGTTTTCTATATTAATATTC+AGG + chr2_4:21958049-21958068 Msa0320970:intron 15.0%
!!! GTTTTCTATATTAATATTCA+GGG + chr2_4:21958050-21958069 Msa0320970:intron 15.0%
!!! TTTTCTATATTAATATTCAG+GGG + chr2_4:21958051-21958070 Msa0320970:intron 15.0%
!! ATAAATCTCAAAAAGAAAAT+GGG + chr2_4:21958262-21958281 Msa0320970:CDS 15.0%
!!! TGATTTATATAGTTTTAGTA+AGG - chr2_4:21956595-21956614 None:intergenic 15.0%
!!! ATATACTTATTTGAACTTTT+TGG + chr2_4:21957526-21957545 Msa0320970:intron 15.0%
!!! TGTTTTCTATATTAATATTC+AGG + chr2_4:21958049-21958068 Msa0320970:intron 15.0%
!!! GTTTTCTATATTAATATTCA+GGG + chr2_4:21958050-21958069 Msa0320970:intron 15.0%
!!! TTTTCTATATTAATATTCAG+GGG + chr2_4:21958051-21958070 Msa0320970:intron 15.0%
!! ATAAATCTCAAAAAGAAAAT+GGG + chr2_4:21958262-21958281 Msa0320970:CDS 15.0%
!! GATATTGTGAATATGTTATT+AGG - chr2_4:21956532-21956551 None:intergenic 20.0%
!!! TTATATAGTTTTAGTAAGGA+GGG - chr2_4:21956591-21956610 None:intergenic 20.0%
!!! TTTATATAGTTTTAGTAAGG+AGG - chr2_4:21956592-21956611 None:intergenic 20.0%
!! ATGCAAAACATATAAATACA+CGG - chr2_4:21957210-21957229 None:intergenic 20.0%
!!! ATGTTTTGCATTATTTTTCA+TGG + chr2_4:21957219-21957238 Msa0320970:intron 20.0%
!! TATTAGATTAAGTGCATATT+TGG - chr2_4:21957450-21957469 None:intergenic 20.0%
!!! ACAATAGATTCATAATTTGT+AGG + chr2_4:21957482-21957501 Msa0320970:intron 20.0%
!!! TTTTTGGCTGTATTTTTAAA+GGG + chr2_4:21957542-21957561 Msa0320970:intron 20.0%
!!! TCTATTTTATTGAATCATGA+AGG + chr2_4:21957575-21957594 Msa0320970:CDS 20.0%
!!! TGAAGTTATTGATGATATTA+AGG + chr2_4:21957640-21957659 Msa0320970:intron 20.0%
!!! TACATTCTGTCAATTTTTTT+TGG - chr2_4:21958238-21958257 None:intergenic 20.0%
!! CATAAATCTCAAAAAGAAAA+TGG + chr2_4:21958261-21958280 Msa0320970:CDS 20.0%
!! GATATTGTGAATATGTTATT+AGG - chr2_4:21956532-21956551 None:intergenic 20.0%
!!! TTATATAGTTTTAGTAAGGA+GGG - chr2_4:21956591-21956610 None:intergenic 20.0%
!!! TTTATATAGTTTTAGTAAGG+AGG - chr2_4:21956592-21956611 None:intergenic 20.0%
!! ATGCAAAACATATAAATACA+CGG - chr2_4:21957210-21957229 None:intergenic 20.0%
!!! ATGTTTTGCATTATTTTTCA+TGG + chr2_4:21957219-21957238 Msa0320970:intron 20.0%
!! TATTAGATTAAGTGCATATT+TGG - chr2_4:21957450-21957469 None:intergenic 20.0%
!!! ACAATAGATTCATAATTTGT+AGG + chr2_4:21957482-21957501 Msa0320970:intron 20.0%
!!! TTTTTGGCTGTATTTTTAAA+GGG + chr2_4:21957542-21957561 Msa0320970:intron 20.0%
!!! TCTATTTTATTGAATCATGA+AGG + chr2_4:21957575-21957594 Msa0320970:CDS 20.0%
!!! TGAAGTTATTGATGATATTA+AGG + chr2_4:21957640-21957659 Msa0320970:intron 20.0%
!!! TACATTCTGTCAATTTTTTT+TGG - chr2_4:21958238-21958257 None:intergenic 20.0%
!! CATAAATCTCAAAAAGAAAA+TGG + chr2_4:21958261-21958280 Msa0320970:CDS 20.0%
! TAATAGAAGCCATAACAATT+AGG + chr2_4:21956661-21956680 Msa0320970:intron 25.0%
! AATAGAAGCCATAACAATTA+GGG + chr2_4:21956662-21956681 Msa0320970:intron 25.0%
!! ATAGTTTTCATCATAAGGTT+GGG - chr2_4:21956777-21956796 None:intergenic 25.0%
! AGAAATCATCTCATCAAAAT+CGG - chr2_4:21957070-21957089 None:intergenic 25.0%
! ACATATAAATACACGGATAA+TGG - chr2_4:21957203-21957222 None:intergenic 25.0%
!!! TGCATTATTTTTCATGGATT+TGG + chr2_4:21957225-21957244 Msa0320970:intron 25.0%
!! TTCATGTTTGGTAATTTGAT+TGG - chr2_4:21957298-21957317 None:intergenic 25.0%
! CAAACATACATTAGAGTAAA+TGG - chr2_4:21957328-21957347 None:intergenic 25.0%
! CATTTACTCTAATGTATGTT+TGG + chr2_4:21957326-21957345 Msa0320970:intron 25.0%
!! ATTTTCAACAAAATCACAGT+CGG + chr2_4:21957417-21957436 Msa0320970:intron 25.0%
!!! CTTTTTGGCTGTATTTTTAA+AGG + chr2_4:21957541-21957560 Msa0320970:intron 25.0%
!! ATCGAATATTTTCAATCCAA+AGG + chr2_4:21957851-21957870 Msa0320970:CDS 25.0%
!! ATTTTCTTTGCAAGAAGTTA+AGG - chr2_4:21958174-21958193 None:intergenic 25.0%
! AATTGATGTTCAAAAACAGA+TGG - chr2_4:21958361-21958380 None:intergenic 25.0%
! TAATAGAAGCCATAACAATT+AGG + chr2_4:21956661-21956680 Msa0320970:intron 25.0%
! AATAGAAGCCATAACAATTA+GGG + chr2_4:21956662-21956681 Msa0320970:intron 25.0%
!! ATAGTTTTCATCATAAGGTT+GGG - chr2_4:21956777-21956796 None:intergenic 25.0%
! AGAAATCATCTCATCAAAAT+CGG - chr2_4:21957070-21957089 None:intergenic 25.0%
! ACATATAAATACACGGATAA+TGG - chr2_4:21957203-21957222 None:intergenic 25.0%
!!! TGCATTATTTTTCATGGATT+TGG + chr2_4:21957225-21957244 Msa0320970:intron 25.0%
!! TTCATGTTTGGTAATTTGAT+TGG - chr2_4:21957298-21957317 None:intergenic 25.0%
! CAAACATACATTAGAGTAAA+TGG - chr2_4:21957328-21957347 None:intergenic 25.0%
! CATTTACTCTAATGTATGTT+TGG + chr2_4:21957326-21957345 Msa0320970:intron 25.0%
!! ATTTTCAACAAAATCACAGT+CGG + chr2_4:21957417-21957436 Msa0320970:intron 25.0%
!!! CTTTTTGGCTGTATTTTTAA+AGG + chr2_4:21957541-21957560 Msa0320970:intron 25.0%
!! ATCGAATATTTTCAATCCAA+AGG + chr2_4:21957851-21957870 Msa0320970:CDS 25.0%
!! ATTTTCTTTGCAAGAAGTTA+AGG - chr2_4:21958174-21958193 None:intergenic 25.0%
! AATTGATGTTCAAAAACAGA+TGG - chr2_4:21958361-21958380 None:intergenic 25.0%
ATCTAAAAGAGAGAGAATGA+GGG - chr2_4:21956562-21956581 None:intergenic 30.0%
ATAGAAGCCATAACAATTAG+GGG + chr2_4:21956663-21956682 Msa0320970:intron 30.0%
! CATAGTTTTCATCATAAGGT+TGG - chr2_4:21956778-21956797 None:intergenic 30.0%
ACCTTATGATGAAAACTATG+AGG + chr2_4:21956778-21956797 Msa0320970:CDS 30.0%
! TCCTCATAGTTTTCATCATA+AGG - chr2_4:21956782-21956801 None:intergenic 30.0%
AATCTGTATCCTCTAATGTT+GGG - chr2_4:21956826-21956845 None:intergenic 30.0%
AAATCTGTATCCTCTAATGT+TGG - chr2_4:21956827-21956846 None:intergenic 30.0%
AACATTAGAGGATACAGATT+TGG + chr2_4:21956826-21956845 Msa0320970:CDS 30.0%
TTGGATAATCTCTTATCCTT+TGG + chr2_4:21956845-21956864 Msa0320970:CDS 30.0%
!! AGTCAAAGAATGGATTAAGA+AGG + chr2_4:21956916-21956935 Msa0320970:CDS 30.0%
TTTGGATTTCCTATAGTAGT+CGG + chr2_4:21957243-21957262 Msa0320970:intron 30.0%
TCTAATGTATGTTTGGATTG+TGG + chr2_4:21957333-21957352 Msa0320970:intron 30.0%
!! GTATTTTTAAAGGGATGTGA+TGG + chr2_4:21957551-21957570 Msa0320970:intron 30.0%
ATATTCTTACAACTGCTTCA+AGG + chr2_4:21957705-21957724 Msa0320970:CDS 30.0%
TATTCTTACAACTGCTTCAA+GGG + chr2_4:21957706-21957725 Msa0320970:CDS 30.0%
TATTGACAAAGAAGCTGAAA+TGG + chr2_4:21957796-21957815 Msa0320970:CDS 30.0%
GGATTGAAAATATTCGATCA+AGG - chr2_4:21957849-21957868 None:intergenic 30.0%
GAGAATGACTTACAAAGATA+TGG + chr2_4:21958000-21958019 Msa0320970:intron 30.0%
AAAGATATGGACCAATTTGA+TGG + chr2_4:21958013-21958032 Msa0320970:intron 30.0%
TGGTTAGTAAACCATCAAAT+TGG - chr2_4:21958027-21958046 None:intergenic 30.0%
!! GGTATTTTGGATCTAATGAT+GGG - chr2_4:21958153-21958172 None:intergenic 30.0%
!! AGGTATTTTGGATCTAATGA+TGG - chr2_4:21958154-21958173 None:intergenic 30.0%
!! TGCAAGAAGTTAAGGTATTT+TGG - chr2_4:21958166-21958185 None:intergenic 30.0%
TCTTGCAAAGAAAATGTAGA+TGG + chr2_4:21958179-21958198 Msa0320970:CDS 30.0%
CTTGCAAAGAAAATGTAGAT+GGG + chr2_4:21958180-21958199 Msa0320970:CDS 30.0%
!! AGCATTGACAAAATTGCAAT+TGG - chr2_4:21958452-21958471 None:intergenic 30.0%
ATCTAAAAGAGAGAGAATGA+GGG - chr2_4:21956562-21956581 None:intergenic 30.0%
ATAGAAGCCATAACAATTAG+GGG + chr2_4:21956663-21956682 Msa0320970:intron 30.0%
! CATAGTTTTCATCATAAGGT+TGG - chr2_4:21956778-21956797 None:intergenic 30.0%
ACCTTATGATGAAAACTATG+AGG + chr2_4:21956778-21956797 Msa0320970:CDS 30.0%
! TCCTCATAGTTTTCATCATA+AGG - chr2_4:21956782-21956801 None:intergenic 30.0%
AATCTGTATCCTCTAATGTT+GGG - chr2_4:21956826-21956845 None:intergenic 30.0%
AAATCTGTATCCTCTAATGT+TGG - chr2_4:21956827-21956846 None:intergenic 30.0%
AACATTAGAGGATACAGATT+TGG + chr2_4:21956826-21956845 Msa0320970:CDS 30.0%
TTGGATAATCTCTTATCCTT+TGG + chr2_4:21956845-21956864 Msa0320970:CDS 30.0%
!! AGTCAAAGAATGGATTAAGA+AGG + chr2_4:21956916-21956935 Msa0320970:CDS 30.0%
TTTGGATTTCCTATAGTAGT+CGG + chr2_4:21957243-21957262 Msa0320970:intron 30.0%
TCTAATGTATGTTTGGATTG+TGG + chr2_4:21957333-21957352 Msa0320970:intron 30.0%
!! GTATTTTTAAAGGGATGTGA+TGG + chr2_4:21957551-21957570 Msa0320970:intron 30.0%
ATATTCTTACAACTGCTTCA+AGG + chr2_4:21957705-21957724 Msa0320970:CDS 30.0%
TATTCTTACAACTGCTTCAA+GGG + chr2_4:21957706-21957725 Msa0320970:CDS 30.0%
TATTGACAAAGAAGCTGAAA+TGG + chr2_4:21957796-21957815 Msa0320970:CDS 30.0%
GGATTGAAAATATTCGATCA+AGG - chr2_4:21957849-21957868 None:intergenic 30.0%
GAGAATGACTTACAAAGATA+TGG + chr2_4:21958000-21958019 Msa0320970:intron 30.0%
AAAGATATGGACCAATTTGA+TGG + chr2_4:21958013-21958032 Msa0320970:intron 30.0%
TGGTTAGTAAACCATCAAAT+TGG - chr2_4:21958027-21958046 None:intergenic 30.0%
!! GGTATTTTGGATCTAATGAT+GGG - chr2_4:21958153-21958172 None:intergenic 30.0%
!! AGGTATTTTGGATCTAATGA+TGG - chr2_4:21958154-21958173 None:intergenic 30.0%
!! TGCAAGAAGTTAAGGTATTT+TGG - chr2_4:21958166-21958185 None:intergenic 30.0%
TCTTGCAAAGAAAATGTAGA+TGG + chr2_4:21958179-21958198 Msa0320970:CDS 30.0%
CTTGCAAAGAAAATGTAGAT+GGG + chr2_4:21958180-21958199 Msa0320970:CDS 30.0%
!! AGCATTGACAAAATTGCAAT+TGG - chr2_4:21958452-21958471 None:intergenic 30.0%
! CATGTCATTTGTGTTCTACT+AGG + chr2_4:21956485-21956504 Msa0320970:CDS 35.0%
TATTAGGATGTGACAAACGT+GGG - chr2_4:21956516-21956535 None:intergenic 35.0%
TTATTAGGATGTGACAAACG+TGG - chr2_4:21956517-21956536 None:intergenic 35.0%
CATCTAAAAGAGAGAGAATG+AGG - chr2_4:21956563-21956582 None:intergenic 35.0%
TAGAAGCCATAACAATTAGG+GGG + chr2_4:21956664-21956683 Msa0320970:intron 35.0%
GAACCAAAAGAAGCAACAAT+CGG - chr2_4:21956730-21956749 None:intergenic 35.0%
! GCAAGATGGATTCAAATTGA+GGG - chr2_4:21956889-21956908 None:intergenic 35.0%
! TGCAAGATGGATTCAAATTG+AGG - chr2_4:21956890-21956909 None:intergenic 35.0%
! TTTGACTTTGCTATGCAAGA+TGG - chr2_4:21956903-21956922 None:intergenic 35.0%
TGCATAGCAAAGTCAAAGAA+TGG + chr2_4:21956906-21956925 Msa0320970:CDS 35.0%
TTGGATTTCCTATAGTAGTC+GGG + chr2_4:21957244-21957263 Msa0320970:intron 35.0%
AATGGTCTAGACTTCATGTT+TGG - chr2_4:21957310-21957329 None:intergenic 35.0%
TGTTTGGATTGTGGTAAGTT+TGG + chr2_4:21957342-21957361 Msa0320970:intron 35.0%
TGAATCATGAAGGAAGTGAA+AGG + chr2_4:21957585-21957604 Msa0320970:CDS 35.0%
TCAAGGAAGTAATGTTCTCA+TGG - chr2_4:21957832-21957851 None:intergenic 35.0%
TACAGGTTATACAACTACCA+AGG + chr2_4:21958112-21958131 Msa0320970:intron 35.0%
TTATACAACTACCAAGGAAC+AGG + chr2_4:21958118-21958137 Msa0320970:intron 35.0%
ATCAAGATCCACATATTCTG+TGG - chr2_4:21958206-21958225 None:intergenic 35.0%
! TTCAAAAACAGATGGTGTTG+TGG - chr2_4:21958353-21958372 None:intergenic 35.0%
! GCTGTTTCAATGTCAAAGTT+TGG + chr2_4:21958382-21958401 Msa0320970:CDS 35.0%
! CATGTCATTTGTGTTCTACT+AGG + chr2_4:21956485-21956504 Msa0320970:CDS 35.0%
TATTAGGATGTGACAAACGT+GGG - chr2_4:21956516-21956535 None:intergenic 35.0%
TTATTAGGATGTGACAAACG+TGG - chr2_4:21956517-21956536 None:intergenic 35.0%
CATCTAAAAGAGAGAGAATG+AGG - chr2_4:21956563-21956582 None:intergenic 35.0%
TAGAAGCCATAACAATTAGG+GGG + chr2_4:21956664-21956683 Msa0320970:intron 35.0%
GAACCAAAAGAAGCAACAAT+CGG - chr2_4:21956730-21956749 None:intergenic 35.0%
! GCAAGATGGATTCAAATTGA+GGG - chr2_4:21956889-21956908 None:intergenic 35.0%
! TGCAAGATGGATTCAAATTG+AGG - chr2_4:21956890-21956909 None:intergenic 35.0%
! TTTGACTTTGCTATGCAAGA+TGG - chr2_4:21956903-21956922 None:intergenic 35.0%
TGCATAGCAAAGTCAAAGAA+TGG + chr2_4:21956906-21956925 Msa0320970:CDS 35.0%
TTGGATTTCCTATAGTAGTC+GGG + chr2_4:21957244-21957263 Msa0320970:intron 35.0%
AATGGTCTAGACTTCATGTT+TGG - chr2_4:21957310-21957329 None:intergenic 35.0%
TGTTTGGATTGTGGTAAGTT+TGG + chr2_4:21957342-21957361 Msa0320970:intron 35.0%
TGAATCATGAAGGAAGTGAA+AGG + chr2_4:21957585-21957604 Msa0320970:CDS 35.0%
TCAAGGAAGTAATGTTCTCA+TGG - chr2_4:21957832-21957851 None:intergenic 35.0%
TACAGGTTATACAACTACCA+AGG + chr2_4:21958112-21958131 Msa0320970:intron 35.0%
TTATACAACTACCAAGGAAC+AGG + chr2_4:21958118-21958137 Msa0320970:intron 35.0%
ATCAAGATCCACATATTCTG+TGG - chr2_4:21958206-21958225 None:intergenic 35.0%
! TTCAAAAACAGATGGTGTTG+TGG - chr2_4:21958353-21958372 None:intergenic 35.0%
! GCTGTTTCAATGTCAAAGTT+TGG + chr2_4:21958382-21958401 Msa0320970:CDS 35.0%
ATCTCACCCCCTAATTGTTA+TGG - chr2_4:21956673-21956692 None:intergenic 40.0%
TCTACAAGTCCCAACATTAG+AGG + chr2_4:21956814-21956833 Msa0320970:CDS 40.0%
! GCTTTTACGGTAGTAACCAA+AGG - chr2_4:21956864-21956883 None:intergenic 40.0%
! CAAATTGAGGGCAGCTTTTA+CGG - chr2_4:21956877-21956896 None:intergenic 40.0%
! AATCATGGAAGTGCAACCTT+AGG - chr2_4:21956964-21956983 None:intergenic 40.0%
ACTTCCATGATTGCTCTGTT+AGG + chr2_4:21956972-21956991 Msa0320970:CDS 40.0%
CTTCCATGATTGCTCTGTTA+GGG + chr2_4:21956973-21956992 Msa0320970:CDS 40.0%
! AACACTGATTGCTAGACTAC+AGG - chr2_4:21957273-21957292 None:intergenic 40.0%
CAAGTAAGCAAGACATTGAG+AGG + chr2_4:21957614-21957633 Msa0320970:CDS 40.0%
! TCAGCACAAGAGACTGTTTT+AGG - chr2_4:21957686-21957705 None:intergenic 40.0%
CATATTGGTCAGTCCCTTAT+GGG + chr2_4:21957753-21957772 Msa0320970:CDS 40.0%
TTCCATCTTTCCTCCCATAA+GGG - chr2_4:21957769-21957788 None:intergenic 40.0%
TTTCCATCTTTCCTCCCATA+AGG - chr2_4:21957770-21957789 None:intergenic 40.0%
AGAAGCTGAAATGGTACCAA+TGG + chr2_4:21957805-21957824 Msa0320970:CDS 40.0%
GAAGCTGAAATGGTACCAAT+GGG + chr2_4:21957806-21957825 Msa0320970:CDS 40.0%
CTGAGAGAACAACTAAGTCA+AGG - chr2_4:21957886-21957905 None:intergenic 40.0%
CTTGACTTAGTTGTTCTCTC+AGG + chr2_4:21957884-21957903 Msa0320970:CDS 40.0%
! TATTCAGGGGCACATACTAT+TGG + chr2_4:21958064-21958083 Msa0320970:intron 40.0%
ACTATTGGAAGGACTACTTG+TGG + chr2_4:21958079-21958098 Msa0320970:intron 40.0%
! CTTGTGGCTCTATTCAATAC+AGG + chr2_4:21958095-21958114 Msa0320970:intron 40.0%
!! TTTGGATCTAATGATGGGTC+TGG - chr2_4:21958148-21958167 None:intergenic 40.0%
GAAACAAGTGGAGAAGTTCT+TGG - chr2_4:21958322-21958341 None:intergenic 40.0%
TTCTCCACTTGTTTCAGCAT+TGG + chr2_4:21958327-21958346 Msa0320970:CDS 40.0%
!! GGTGTCATTGATGTTCTTAC+TGG + chr2_4:21958403-21958422 Msa0320970:CDS 40.0%
GTTCTTACTGGAGATGATGA+AGG + chr2_4:21958415-21958434 Msa0320970:CDS 40.0%
GAGATGATGAAGGAGAAATC+AGG + chr2_4:21958425-21958444 Msa0320970:CDS 40.0%
ATCTCACCCCCTAATTGTTA+TGG - chr2_4:21956673-21956692 None:intergenic 40.0%
TCTACAAGTCCCAACATTAG+AGG + chr2_4:21956814-21956833 Msa0320970:CDS 40.0%
! GCTTTTACGGTAGTAACCAA+AGG - chr2_4:21956864-21956883 None:intergenic 40.0%
! CAAATTGAGGGCAGCTTTTA+CGG - chr2_4:21956877-21956896 None:intergenic 40.0%
! AATCATGGAAGTGCAACCTT+AGG - chr2_4:21956964-21956983 None:intergenic 40.0%
ACTTCCATGATTGCTCTGTT+AGG + chr2_4:21956972-21956991 Msa0320970:CDS 40.0%
CTTCCATGATTGCTCTGTTA+GGG + chr2_4:21956973-21956992 Msa0320970:CDS 40.0%
! AACACTGATTGCTAGACTAC+AGG - chr2_4:21957273-21957292 None:intergenic 40.0%
CAAGTAAGCAAGACATTGAG+AGG + chr2_4:21957614-21957633 Msa0320970:CDS 40.0%
! TCAGCACAAGAGACTGTTTT+AGG - chr2_4:21957686-21957705 None:intergenic 40.0%
CATATTGGTCAGTCCCTTAT+GGG + chr2_4:21957753-21957772 Msa0320970:CDS 40.0%
TTCCATCTTTCCTCCCATAA+GGG - chr2_4:21957769-21957788 None:intergenic 40.0%
TTTCCATCTTTCCTCCCATA+AGG - chr2_4:21957770-21957789 None:intergenic 40.0%
AGAAGCTGAAATGGTACCAA+TGG + chr2_4:21957805-21957824 Msa0320970:CDS 40.0%
GAAGCTGAAATGGTACCAAT+GGG + chr2_4:21957806-21957825 Msa0320970:CDS 40.0%
CTGAGAGAACAACTAAGTCA+AGG - chr2_4:21957886-21957905 None:intergenic 40.0%
CTTGACTTAGTTGTTCTCTC+AGG + chr2_4:21957884-21957903 Msa0320970:CDS 40.0%
! TATTCAGGGGCACATACTAT+TGG + chr2_4:21958064-21958083 Msa0320970:intron 40.0%
ACTATTGGAAGGACTACTTG+TGG + chr2_4:21958079-21958098 Msa0320970:intron 40.0%
! CTTGTGGCTCTATTCAATAC+AGG + chr2_4:21958095-21958114 Msa0320970:intron 40.0%
!! TTTGGATCTAATGATGGGTC+TGG - chr2_4:21958148-21958167 None:intergenic 40.0%
GAAACAAGTGGAGAAGTTCT+TGG - chr2_4:21958322-21958341 None:intergenic 40.0%
TTCTCCACTTGTTTCAGCAT+TGG + chr2_4:21958327-21958346 Msa0320970:CDS 40.0%
!! GGTGTCATTGATGTTCTTAC+TGG + chr2_4:21958403-21958422 Msa0320970:CDS 40.0%
GTTCTTACTGGAGATGATGA+AGG + chr2_4:21958415-21958434 Msa0320970:CDS 40.0%
GAGATGATGAAGGAGAAATC+AGG + chr2_4:21958425-21958444 Msa0320970:CDS 40.0%
CCAAAAGAAGCAACAATCGG+AGG - chr2_4:21956727-21956746 None:intergenic 45.0%
!! CCTCCGATTGTTGCTTCTTT+TGG + chr2_4:21956724-21956743 Msa0320970:intron 45.0%
! CTTTAGCAGCTAGTCTCCTA+AGG + chr2_4:21956945-21956964 Msa0320970:CDS 45.0%
CATGATTGCTCTGTTAGGGT+GGG + chr2_4:21956977-21956996 Msa0320970:intron 45.0%
ACAGGATTCCCGACTACTAT+AGG - chr2_4:21957255-21957274 None:intergenic 45.0%
!! TTGAGTTGGGTGGACCATAT+TGG + chr2_4:21957738-21957757 Msa0320970:CDS 45.0%
CCATAAGGGACTGACCAATA+TGG - chr2_4:21957755-21957774 None:intergenic 45.0%
CCATATTGGTCAGTCCCTTA+TGG + chr2_4:21957752-21957771 Msa0320970:CDS 45.0%
GTAATGTTCTCATGGCCCAT+TGG - chr2_4:21957824-21957843 None:intergenic 45.0%
TAGATGGGCCACAGAATATG+TGG + chr2_4:21958195-21958214 Msa0320970:CDS 45.0%
CCAAAAGAAGCAACAATCGG+AGG - chr2_4:21956727-21956746 None:intergenic 45.0%
!! CCTCCGATTGTTGCTTCTTT+TGG + chr2_4:21956724-21956743 Msa0320970:intron 45.0%
! CTTTAGCAGCTAGTCTCCTA+AGG + chr2_4:21956945-21956964 Msa0320970:CDS 45.0%
CATGATTGCTCTGTTAGGGT+GGG + chr2_4:21956977-21956996 Msa0320970:intron 45.0%
ACAGGATTCCCGACTACTAT+AGG - chr2_4:21957255-21957274 None:intergenic 45.0%
!! TTGAGTTGGGTGGACCATAT+TGG + chr2_4:21957738-21957757 Msa0320970:CDS 45.0%
CCATAAGGGACTGACCAATA+TGG - chr2_4:21957755-21957774 None:intergenic 45.0%
CCATATTGGTCAGTCCCTTA+TGG + chr2_4:21957752-21957771 Msa0320970:CDS 45.0%
GTAATGTTCTCATGGCCCAT+TGG - chr2_4:21957824-21957843 None:intergenic 45.0%
TAGATGGGCCACAGAATATG+TGG + chr2_4:21958195-21958214 Msa0320970:CDS 45.0%
CCACCCTAACAGAGCAATCA+TGG - chr2_4:21956979-21956998 None:intergenic 50.0%
CCATGATTGCTCTGTTAGGG+TGG + chr2_4:21956976-21956995 Msa0320970:intron 50.0%
AAGGGATGCCACTGTTGAGT+TGG + chr2_4:21957724-21957743 Msa0320970:CDS 50.0%
AGGGATGCCACTGTTGAGTT+GGG + chr2_4:21957725-21957744 Msa0320970:CDS 50.0%
ATTGGTCAGTCCCTTATGGG+AGG + chr2_4:21957756-21957775 Msa0320970:CDS 50.0%
GTCCCTTATGGGAGGAAAGA+TGG + chr2_4:21957764-21957783 Msa0320970:CDS 50.0%
GTCAAGGACAGTCAAGCCTT+TGG - chr2_4:21957870-21957889 None:intergenic 50.0%
! CAGGGGCACATACTATTGGA+AGG + chr2_4:21958068-21958087 Msa0320970:intron 50.0%
GTGGCCAATGCTGAAACAAG+TGG - chr2_4:21958334-21958353 None:intergenic 50.0%
CCACCCTAACAGAGCAATCA+TGG - chr2_4:21956979-21956998 None:intergenic 50.0%
CCATGATTGCTCTGTTAGGG+TGG + chr2_4:21956976-21956995 Msa0320970:intron 50.0%
AAGGGATGCCACTGTTGAGT+TGG + chr2_4:21957724-21957743 Msa0320970:CDS 50.0%
AGGGATGCCACTGTTGAGTT+GGG + chr2_4:21957725-21957744 Msa0320970:CDS 50.0%
ATTGGTCAGTCCCTTATGGG+AGG + chr2_4:21957756-21957775 Msa0320970:CDS 50.0%
GTCCCTTATGGGAGGAAAGA+TGG + chr2_4:21957764-21957783 Msa0320970:CDS 50.0%
GTCAAGGACAGTCAAGCCTT+TGG - chr2_4:21957870-21957889 None:intergenic 50.0%
! CAGGGGCACATACTATTGGA+AGG + chr2_4:21958068-21958087 Msa0320970:intron 50.0%
GTGGCCAATGCTGAAACAAG+TGG - chr2_4:21958334-21958353 None:intergenic 50.0%
!! GATGCCACTGTTGAGTTGGG+TGG + chr2_4:21957728-21957747 Msa0320970:CDS 55.0%
TGGTCCACCCAACTCAACAG+TGG - chr2_4:21957735-21957754 None:intergenic 55.0%
! GGTCTGGTTTGCCTGTTCCT+TGG - chr2_4:21958132-21958151 None:intergenic 55.0%
!! GATGCCACTGTTGAGTTGGG+TGG + chr2_4:21957728-21957747 Msa0320970:CDS 55.0%
TGGTCCACCCAACTCAACAG+TGG - chr2_4:21957735-21957754 None:intergenic 55.0%
! GGTCTGGTTTGCCTGTTCCT+TGG - chr2_4:21958132-21958151 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 21956478 21958477 21956478 ID=Msa0320970;Name=Msa0320970
chr2_4 mRNA 21956478 21958477 21956478 ID=Msa0320970-mRNA-1;Parent=Msa0320970;Name=Msa0320970-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|0.8|0.25|0.2|5|0|312
chr2_4 exon 21956478 21956517 21956478 ID=Msa0320970-mRNA-1:exon:8047;Parent=Msa0320970-mRNA-1
chr2_4 exon 21956765 21956994 21956765 ID=Msa0320970-mRNA-1:exon:8048;Parent=Msa0320970-mRNA-1
chr2_4 exon 21957563 21957643 21957563 ID=Msa0320970-mRNA-1:exon:8049;Parent=Msa0320970-mRNA-1
chr2_4 exon 21957662 21957905 21957662 ID=Msa0320970-mRNA-1:exon:8050;Parent=Msa0320970-mRNA-1
chr2_4 exon 21958134 21958477 21958134 ID=Msa0320970-mRNA-1:exon:8051;Parent=Msa0320970-mRNA-1
chr2_4 CDS 21956478 21956517 21956478 ID=Msa0320970-mRNA-1:cds;Parent=Msa0320970-mRNA-1
chr2_4 CDS 21956765 21956994 21956765 ID=Msa0320970-mRNA-1:cds;Parent=Msa0320970-mRNA-1
chr2_4 CDS 21957563 21957643 21957563 ID=Msa0320970-mRNA-1:cds;Parent=Msa0320970-mRNA-1
chr2_4 CDS 21957662 21957905 21957662 ID=Msa0320970-mRNA-1:cds;Parent=Msa0320970-mRNA-1
chr2_4 CDS 21958134 21958477 21958134 ID=Msa0320970-mRNA-1:cds;Parent=Msa0320970-mRNA-1
Gene Sequence

>Msa0320970

ATGCTAGCATGTCATTTGTGTTCTACTAGGTGCGTCCCACTTAATTCAACCCAACCTTATGATGAAAACTATGAGGATGCTGAGTTCACTCTACAAGTCCCAACATTAGAGGATACAGATTTGGATAATCTCTTATCCTTTGGTTACTACCGTAAAAGCTGCCCTCAATTTGAATCCATCTTGCATAGCAAAGTCAAAGAATGGATTAAGAAGGATTACACTTTAGCAGCTAGTCTCCTAAGGTTGCACTTCCATGATTGCTCTGTTAGGGGATGTGATGGCTCTATTTTATTGAATCATGAAGGAAGTGAAAGGAAATCACAAGTAAGCAAGACATTGAGAGGTTATGAAGCAGAGATAGAGAAACAATGTCCTAAAACAGTCTCTTGTGCTGATATTCTTACAACTGCTTCAAGGGATGCCACTGTTGAGTTGGGTGGACCATATTGGTCAGTCCCTTATGGGAGGAAAGATGGAAAAGTCTCTATTGACAAAGAAGCTGAAATGGTACCAATGGGCCATGAGAACATTACTTCCTTGATCGAATATTTTCAATCCAAAGGCTTGACTGTCCTTGACTTAGTTGTTCTCTCAGGAACAGGCAAACCAGACCCATCATTAGATCCAAAATACCTTAACTTCTTGCAAAGAAAATGTAGATGGGCCACAGAATATGTGGATCTTGATGCAACAACACCAAAAAAAATTGACAGAATGTACTACATAAATCTCAAAAAGAAAATGGGATTGTTAACAACAGATCAATTGTTATATTCTGATCCAAGAACTTCTCCACTTGTTTCAGCATTGGCCACAACACCATCTGTTTTTGAACATCAATTTGCTGTTTCAATGTCAAAGTTTGGTGTCATTGATGTTCTTACTGGAGATGATGAAGGAGAAATCAGGACCAATTGCAATTTTGTCAATGCTTATTGA
Protein sequence

>Msa0320970

MLACHLCSTRCVPLNSTQPYDENYEDAEFTLQVPTLEDTDLDNLLSFGYYRKSCPQFESILHSKVKEWIKKDYTLAASLLRLHFHDCSVRGCDGSILLNHEGSERKSQVSKTLRGYEAEIEKQCPKTVSCADILTTASRDATVELGGPYWSVPYGRKDGKVSIDKEAEMVPMGHENITSLIEYFQSKGLTVLDLVVLSGTGKPDPSLDPKYLNFLQRKCRWATEYVDLDATTPKKIDRMYYINLKKKMGLLTTDQLLYSDPRTSPLVSALATTPSVFEHQFAVSMSKFGVIDVLTGDDEGEIRTNCNFVNAY*