AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0321660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0321660 MtrunA17_Chr2g0298861 87.480 623 35 2 1 580 1 623 0.0 1090
Msa0321660 MtrunA17_Chr7g0223721 77.578 611 106 1 1 580 1 611 0.0 953
Msa0321660 MtrunA17_Chr4g0040931 77.087 611 109 1 1 580 1 611 0.0 949
Msa0321660 MtrunA17_Chr2g0298851 71.732 612 140 2 1 580 1 611 0.0 902
Msa0321660 MtrunA17_Chr7g0223851 68.640 625 160 4 1 590 10 633 0.0 859
Msa0321660 MtrunA17_Chr2g0293581 68.026 613 163 3 1 580 1 613 0.0 849
Msa0321660 MtrunA17_Chr5g0427901 68.006 622 156 3 1 580 1 621 0.0 848
Msa0321660 MtrunA17_Chr7g0233301 71.084 581 167 1 1 580 1 581 0.0 844
Msa0321660 MtrunA17_Chr7g0233311 68.464 612 161 2 1 580 1 612 0.0 843
Msa0321660 MtrunA17_Chr7g0225671 69.868 604 150 3 6 578 13 615 0.0 843
Msa0321660 MtrunA17_Chr7g0221661 68.464 612 161 2 1 581 1 611 0.0 840
Msa0321660 MtrunA17_Chr7g0223801 68.576 611 158 4 1 580 1 608 0.0 832
Msa0321660 MtrunA17_Chr7g0223821 68.293 615 157 4 1 579 1 613 0.0 822
Msa0321660 MtrunA17_Chr7g0223821 45.714 105 46 2 487 580 596 700 1.30e-15 80.9
Msa0321660 MtrunA17_Chr2g0287631 67.647 612 166 2 1 580 1 612 0.0 817
Msa0321660 MtrunA17_Chr4g0012541 65.280 625 172 4 1 590 1 615 0.0 806
Msa0321660 MtrunA17_Chr7g0217971 66.113 602 147 4 1 571 1 576 0.0 791
Msa0321660 MtrunA17_Chr7g0223831 64.506 617 158 3 1 585 1 588 0.0 782
Msa0321660 MtrunA17_Chr7g0215261 65.049 618 148 5 1 585 1 583 0.0 775
Msa0321660 MtrunA17_Chr8g0390821 61.295 664 157 8 1 580 1 648 0.0 765
Msa0321660 MtrunA17_Chr2g0296391 69.434 530 130 2 1 498 1 530 0.0 763
Msa0321660 MtrunA17_Chr7g0228281 64.039 609 143 5 1 578 62 625 0.0 762
Msa0321660 MtrunA17_Chr7g0223181 63.011 611 190 5 1 578 1 608 0.0 756
Msa0321660 MtrunA17_Chr7g0223711 60.450 622 211 5 1 590 1 619 0.0 751
Msa0321660 MtrunA17_Chr4g0031301 62.520 611 193 5 1 578 1 608 0.0 749
Msa0321660 MtrunA17_Chr4g0031321 62.357 611 194 5 1 578 1 608 0.0 744
Msa0321660 MtrunA17_Chr7g0223291 61.702 611 198 5 1 578 1 608 0.0 744
Msa0321660 MtrunA17_Chr8g0350731 60.897 624 210 4 1 593 29 649 0.0 743
Msa0321660 MtrunA17_Chr7g0262821 61.905 609 196 5 1 576 1 606 0.0 742
Msa0321660 MtrunA17_Chr7g0223781 63.032 587 184 2 1 556 1 585 0.0 740
Msa0321660 MtrunA17_Chr7g0223031 61.111 612 205 3 1 581 1 610 0.0 740
Msa0321660 MtrunA17_Chr7g0223731 60.621 612 208 3 1 581 1 610 0.0 737
Msa0321660 MtrunA17_Chr4g0073741 60.383 626 211 6 1 593 1 622 0.0 737
Msa0321660 MtrunA17_Chr7g0223751 59.903 621 215 4 1 590 1 618 0.0 736
Msa0321660 MtrunA17_Chr7g0248131 60.065 616 210 5 1 581 1 615 0.0 732
Msa0321660 MtrunA17_Chr7g0223621 59.477 612 215 3 1 581 1 610 0.0 726
Msa0321660 MtrunA17_Chr7g0223701 58.548 620 217 3 1 581 1 619 0.0 721
Msa0321660 MtrunA17_Chr7g0223741 62.478 581 185 4 1 549 1 580 0.0 717
Msa0321660 MtrunA17_Chr7g0248151 59.283 614 216 3 1 581 1 613 0.0 711
Msa0321660 MtrunA17_Chr2g0293601 71.663 487 134 2 1 486 1 484 0.0 707
Msa0321660 MtrunA17_Chr7g0223631 59.581 621 203 6 1 590 1 604 0.0 704
Msa0321660 MtrunA17_Chr1g0166551 63.432 577 129 5 6 581 2 497 0.0 695
Msa0321660 MtrunA17_Chr2g0331381 64.314 510 147 4 9 516 6 482 0.0 652
Msa0321660 MtrunA17_Chr7g0223541 59.074 540 187 4 74 581 31 568 0.0 624
Msa0321660 MtrunA17_Chr7g0223681 59.887 531 179 4 91 590 2 529 0.0 621
Msa0321660 MtrunA17_Chr2g0293561 76.738 374 87 0 1 374 1 374 0.0 613
Msa0321660 MtrunA17_Chr6g0462601 54.124 582 231 5 1 549 1 579 0.0 608
Msa0321660 MtrunA17_Chr6g0462681 53.265 582 236 5 1 549 1 579 0.0 603
Msa0321660 MtrunA17_Chr2g0284681 52.805 606 230 8 10 576 9 597 0.0 590
Msa0321660 MtrunA17_Chr2g0299821 57.752 516 174 6 1 506 1 482 0.0 578
Msa0321660 MtrunA17_Chr6g0462711 51.361 588 246 6 10 559 11 596 0.0 559
Msa0321660 MtrunA17_Chr8g0388761 49.322 590 256 9 7 560 36 618 0.0 552
Msa0321660 MtrunA17_Chr8g0388671 49.492 590 255 9 7 560 36 618 0.0 550
Msa0321660 MtrunA17_Chr6g0462771 51.300 577 240 6 10 548 10 583 0.0 548
Msa0321660 MtrunA17_Chr6g0462641 50.596 587 247 7 1 548 1 583 0.0 540
Msa0321660 MtrunA17_Chr6g0462611 51.060 519 220 4 63 549 11 527 9.33e-175 511
Msa0321660 MtrunA17_Chr2g0299921 66.497 394 128 2 3 396 1023 1412 1.77e-170 531
Msa0321660 MtrunA17_Chr2g0299921 65.979 388 127 3 4 390 1408 1791 6.15e-158 498
Msa0321660 MtrunA17_Chr2g0299851 64.359 390 135 2 1 390 1030 1415 1.33e-166 514
Msa0321660 MtrunA17_Chr2g0299791 65.039 389 132 2 1 389 1034 1418 1.65e-166 513
Msa0321660 MtrunA17_Chr2g0293271 60.859 419 99 6 21 426 43 409 1.03e-165 480
Msa0321660 MtrunA17_Chr1g0151991 76.976 291 67 0 1 291 1 291 1.05e-165 476
Msa0321660 MtrunA17_Chr2g0299881 63.427 391 135 3 3 389 1032 1418 2.03e-159 496
Msa0321660 MtrunA17_Chr2g0299881 58.904 73 28 1 180 250 1403 1475 1.01e-17 88.2
Msa0321660 MtrunA17_Chr7g0223761 68.713 342 93 3 241 580 3 332 7.44e-151 440
Msa0321660 MtrunA17_Chr7g0245521 77.289 273 62 0 1 273 1 273 1.92e-150 436
Msa0321660 MtrunA17_Chr6g0462861 42.022 633 303 11 6 596 6 616 2.22e-144 434
Msa0321660 MtrunA17_Chr2g0279901 43.686 586 274 13 2 550 49 615 6.16e-142 429
Msa0321660 MtrunA17_Chr4g0049501 41.653 617 297 16 2 579 46 638 4.97e-136 414
Msa0321660 MtrunA17_Chr2g0277021 42.652 558 268 11 9 529 71 613 2.15e-124 385
Msa0321660 MtrunA17_Chr7g0245531 61.415 311 88 2 305 584 2 311 2.08e-122 366
Msa0321660 MtrunA17_Chr1g0152001 59.797 296 88 2 315 580 1 295 1.81e-114 346
Msa0321660 MtrunA17_Chr7g0223671 75.510 196 44 1 74 265 8 203 1.45e-103 313
Msa0321660 MtrunA17_Chr1g0188601 78.534 191 41 0 92 282 1 191 5.91e-103 311
Msa0321660 MtrunA17_Chr6g0462631 48.903 319 120 3 91 408 13 289 4.19e-92 287
Msa0321660 MtrunA17_Chr7g0223771 86.275 153 21 0 93 245 1 153 1.18e-90 278
Msa0321660 MtrunA17_Chr6g0462751 46.667 345 124 4 67 408 12 299 1.75e-90 283
Msa0321660 MtrunA17_Chr1g0188611 54.138 290 95 2 322 580 1 283 2.10e-88 277
Msa0321660 MtrunA17_Chr4g0046881 78.723 141 27 1 135 275 14 151 9.34e-73 231
Msa0321660 MtrunA17_Chr4g0004331 31.887 461 297 8 9 466 3 449 9.46e-71 246
Msa0321660 MtrunA17_Chr2g0331901 32.579 442 283 7 9 449 3 430 1.60e-70 245
Msa0321660 MtrunA17_Chr7g0223861 82.443 131 23 0 78 208 4 134 2.17e-70 224
Msa0321660 MtrunA17_Chr5g0394391 30.934 514 293 14 10 473 30 531 1.07e-67 231
Msa0321660 MtrunA17_Chr6g0462791 61.143 175 67 1 109 283 2 175 2.77e-67 218
Msa0321660 MtrunA17_Chr7g0245541 62.644 174 64 1 407 580 22 194 1.77e-63 209
Msa0321660 MtrunA17_Chr3g0098761 32.170 401 265 5 9 407 3 398 8.80e-63 222
Msa0321660 MtrunA17_Chr5g0444001 32.170 401 267 4 9 408 3 399 1.80e-62 217
Msa0321660 MtrunA17_Chr3g0120101 30.273 403 259 7 9 393 26 424 8.63e-55 201
Msa0321660 MtrunA17_Chr6g0462701 53.216 171 56 1 100 270 22 168 4.00e-51 174
Msa0321660 MtrunA17_Chr4g0064621 29.865 519 299 17 10 472 141 650 1.31e-48 181
Msa0321660 MtrunA17_Chr6g0481391 68.033 122 36 1 38 159 13 131 4.31e-48 165
Msa0321660 MtrunA17_Chr4g0060241 28.767 511 284 19 10 465 21 506 3.76e-44 166
Msa0321660 MtrunA17_Chr5g0394221 37.276 279 133 6 280 517 40 317 6.96e-42 155
Msa0321660 MtrunA17_Chr5g0394231 37.004 227 92 4 74 299 5 181 1.13e-37 138
Msa0321660 MtrunA17_Chr7g0223871 55.224 134 57 1 460 590 7 140 4.90e-36 132
Msa0321660 MtrunA17_Chr4g0046871 80.822 73 14 0 336 408 6 78 2.91e-33 123
Msa0321660 MtrunA17_Chr6g0481381 73.973 73 19 0 177 249 2 74 2.52e-31 120
Msa0321660 MtrunA17_Chr6g0481381 56.250 96 42 0 484 579 75 170 6.17e-27 108
Msa0321660 MtrunA17_Chr5g0442171 28.049 328 186 8 170 456 1 319 1.77e-30 122
Msa0321660 MtrunA17_Chr1g0188591 72.603 73 20 0 1 73 1 73 2.45e-30 114
Msa0321660 MtrunA17_Chr7g0223791 48.485 132 59 1 410 541 58 180 2.64e-30 117
Msa0321660 MtrunA17_Chr7g0223791 59.756 82 25 3 56 137 18 91 1.58e-20 89.7
Msa0321660 MtrunA17_Chr7g0223691 67.123 73 24 0 1 73 1 73 6.31e-29 109
Msa0321660 MtrunA17_Chr6g0462691 43.662 142 48 2 322 431 1 142 3.26e-27 108
Msa0321660 MtrunA17_Chr7g0223881 54.878 82 36 1 281 362 2 82 1.40e-24 97.8
Msa0321660 MtrunA17_Chr6g0462621 61.644 73 26 1 1 73 1 71 1.56e-22 91.7
Msa0321660 MtrunA17_Chr7g0221651 48.193 83 43 0 215 297 1 83 7.33e-21 87.4
Msa0321660 MtrunA17_Chr5g0442161 31.579 152 97 4 10 157 29 177 2.31e-16 77.8
Msa0321660 MtrunA17_Chr6g0462801 57.576 66 23 1 322 382 1 66 2.83e-15 71.2
Msa0321660 MtrunA17_Chr2g0299891 68.889 45 14 0 315 359 1 45 4.33e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0321660 AT3G12580.1 62.848 611 191 5 1 578 1 608 0.0 756
Msa0321660 AT5G02500.1 62.029 611 196 5 1 578 1 608 0.0 746
Msa0321660 AT5G02490.1 61.047 611 202 5 1 578 1 608 0.0 736
Msa0321660 AT3G09440.1 60.720 611 204 5 1 578 1 608 0.0 729
Msa0321660 AT3G09440.4 60.720 611 204 5 1 578 1 608 0.0 729
Msa0321660 AT3G09440.2 60.720 611 204 5 1 578 1 608 0.0 729
Msa0321660 AT3G09440.3 60.720 611 204 5 1 578 1 608 0.0 729
Msa0321660 AT1G16030.1 60.393 611 205 6 1 578 1 607 0.0 723
Msa0321660 AT1G56410.1 60.884 611 201 6 1 578 1 606 0.0 719
Msa0321660 AT5G02500.2 71.979 389 107 1 1 389 1 387 0.0 589
Msa0321660 AT5G28540.1 50.165 606 258 10 7 576 35 632 0.0 566
Msa0321660 AT5G42020.1 49.752 605 262 9 7 576 35 632 0.0 564
Msa0321660 AT5G42020.3 49.752 605 262 9 7 576 40 637 0.0 564
Msa0321660 AT1G09080.1 50.166 604 260 8 7 576 50 646 0.0 547
Msa0321660 AT1G09080.2 50.166 604 260 8 7 576 40 636 0.0 547
Msa0321660 AT5G42020.2 48.925 558 225 12 7 517 35 579 5.02e-168 494
Msa0321660 AT5G09590.1 45.607 535 237 13 9 506 59 576 1.93e-133 407
Msa0321660 AT4G37910.1 42.960 554 269 11 9 528 54 594 5.40e-133 406
Msa0321660 AT4G37910.2 42.960 554 269 11 9 528 54 594 5.40e-133 406
Msa0321660 AT4G24280.1 42.218 559 271 11 8 529 79 622 4.34e-125 387
Msa0321660 AT5G49910.1 42.397 559 270 11 8 529 79 622 1.47e-124 386
Msa0321660 AT1G79930.2 32.805 442 282 5 9 449 3 430 1.02e-73 253
Msa0321660 AT1G79930.1 32.805 442 282 5 9 449 3 430 2.30e-73 253
Msa0321660 AT1G79920.1 33.032 442 281 5 9 449 3 430 4.17e-73 250
Msa0321660 AT1G79920.3 33.032 442 281 5 9 449 3 430 5.27e-73 250
Msa0321660 AT1G79920.2 33.032 442 281 5 9 449 3 430 5.27e-73 250
Msa0321660 AT1G79920.4 33.032 442 281 5 9 449 3 430 5.27e-73 250
Msa0321660 AT1G11660.4 33.499 403 260 5 9 408 3 400 8.16e-68 236
Msa0321660 AT1G11660.3 33.499 403 260 5 9 408 3 400 8.16e-68 236
Msa0321660 AT1G11660.1 33.499 403 260 5 9 408 3 400 8.16e-68 236
Msa0321660 AT2G32120.1 31.323 514 286 14 10 476 31 524 3.68e-63 219
Msa0321660 AT2G32120.2 31.323 514 286 14 10 476 31 524 3.68e-63 219
Msa0321660 AT1G11660.2 33.607 366 237 4 46 408 1 363 1.17e-60 216
Msa0321660 AT4G16660.1 28.690 481 305 13 6 461 23 490 1.82e-54 200
Msa0321660 AT4G16660.2 28.690 481 305 13 6 461 47 514 2.05e-54 200

Find 166 sgRNAs with CRISPR-Local

Find 2 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGATTGCAGAGGCTTATT+TGG 0.170727 2_4:-23196016 Msa0321660:CDS
CCTGATGAGGCTGTTGATTA+TGG 0.215785 2_4:-23195301 Msa0321660:CDS
ATGGATGCTGGTGCCATTGC+TGG 0.234093 2_4:-23195925 Msa0321660:CDS
CGCAATAGTTCCTGCACATT+TGG 0.250143 2_4:+23195364 None:intergenic
GTGTCTCTCCTCACAATTAA+AGG 0.259274 2_4:-23195775 Msa0321660:CDS
TCAGATGCTAAGAGGTTAAT+TGG 0.263493 2_4:-23196201 Msa0321660:intron
TTTGAAGTTAAAGCCACTTC+TGG 0.288192 2_4:-23195745 Msa0321660:CDS
AAGGACACTCTCTTTCAAAT+TGG 0.288998 2_4:-23195581 Msa0321660:CDS
ACTTTGTGCAAGAATTTAAT+AGG 0.318140 2_4:-23195675 Msa0321660:CDS
CTTCAATCAAGGCACTATAT+TGG 0.324611 2_4:+23195092 None:intergenic
AACTCACAAAACACCGTCTT+TGG 0.327171 2_4:-23197108 Msa0321660:intron
CTGCAATATTGATGGAGGTT+AGG 0.340762 2_4:-23188809 None:intergenic
TCTTCATACACTCATTAAAA+AGG 0.345773 2_4:+23195470 None:intergenic
GGATAACCTTTCTTTGTCAT+TGG 0.352083 2_4:+23194886 None:intergenic
TGGCCGTTCAAGGTTATTGC+TGG 0.368855 2_4:-23196135 Msa0321660:CDS
CCATAATCAACAGCCTCATC+AGG 0.382710 2_4:+23195301 None:intergenic
GGAGTTGGTGGTTGAGGAAT+GGG 0.387266 2_4:-23188851 Msa0321660:CDS
CTCAACCTCCTCAAGGCTCT+TGG 0.389032 2_4:+23195625 None:intergenic
ACGTCTCGCAGCACCAAGTT+TGG 0.390269 2_4:+23195232 None:intergenic
CATTAAGAATGTTCCAAACT+TGG 0.400175 2_4:-23195245 Msa0321660:CDS
GAAATCGAGTTGGTAGAGAT+TGG 0.405593 2_4:-23188952 Msa0321660:CDS
TGAGATTGATTATTTATATG+AGG 0.407076 2_4:-23195548 Msa0321660:CDS
TATGATTGCTGCAATATTGA+TGG 0.409916 2_4:-23188817 Msa0321660:CDS
GTTGGTAGAGATTGGAGTTA+TGG 0.417341 2_4:-23188944 Msa0321660:CDS
GGAAAAGGAGTTGGTGGTTG+AGG 0.419585 2_4:-23188857 Msa0321660:CDS
TGAAGGATGTAGGAACGATT+GGG 0.422397 2_4:-23189057 Msa0321660:CDS
TGGAGTTATGGCGGAGGATG+AGG 0.423539 2_4:-23188932 Msa0321660:CDS
CTGAGATACCAAGTGACAAT+GGG 0.427519 2_4:+23195206 None:intergenic
TGCAATATTGATGGAGGTTA+GGG 0.429483 2_4:-23188808 None:intergenic
CCATTGCTGGCCTTAACGTT+GGG 0.430331 2_4:-23195912 Msa0321660:CDS
GGTGGTTGAGGAATGGGTTG+TGG 0.430364 2_4:-23188845 Msa0321660:CDS
TCATTACCTTGGCCTTCCTT+AGG 0.431184 2_4:+23194801 None:intergenic
TATATTGTTGTGGCCGTTCA+AGG 0.432647 2_4:-23196145 Msa0321660:CDS
TGATTATCTTCCCAACGTTA+AGG 0.434328 2_4:+23195902 None:intergenic
TGCCTTGATTGAAGTTTATG+AGG 0.441002 2_4:-23195083 Msa0321660:CDS
ATGAGATTTCCTCGGCATAA+AGG 0.441258 2_4:+23196061 None:intergenic
CTGATGAGGCTGTTGATTAT+GGG 0.443704 2_4:-23195300 Msa0321660:CDS
AGGAGTTGGTGGTTGAGGAA+TGG 0.445545 2_4:-23188852 Msa0321660:CDS
CATACTCATGTGTCGCTGTT+TGG 0.450568 2_4:-23197257 Msa0321660:CDS
CTATTGCGTAACTTCTTCAA+TGG 0.452833 2_4:-23195349 Msa0321660:CDS
TAAATAATCAATCTCAATAG+TGG 0.454560 2_4:+23195555 None:intergenic
TTGTCTTTGATCTTGGTGGT+GGG 0.456314 2_4:-23195807 Msa0321660:CDS
TTTGTCTTTGATCTTGGTGG+TGG 0.458128 2_4:-23195808 Msa0321660:CDS
GTTGAGAACTTCTTGTGAAA+AGG 0.462126 2_4:-23195608 Msa0321660:CDS
ATCTATTGCATTCATTACCT+TGG 0.463960 2_4:+23194790 None:intergenic
GAAGAATGCTCTAAAGAGTA+AGG 0.468191 2_4:-23194750 Msa0321660:CDS
ACTGCTGCAGCTATTGCATA+TGG 0.476452 2_4:-23195871 Msa0321660:CDS
CTTCTGGAAACACTCACCTC+GGG 0.477285 2_4:-23195729 Msa0321660:CDS
TGTTCCTCGTGGCCATCCCT+TGG 0.477913 2_4:-23194996 Msa0321660:CDS
TATTGCGTAACTTCTTCAAT+GGG 0.481757 2_4:-23195348 Msa0321660:CDS
GTGAAGGATGTAGGAACGAT+TGG 0.483336 2_4:-23189058 Msa0321660:CDS
TTGAAGTTTATGAGGGTGAG+AGG 0.487743 2_4:-23195075 Msa0321660:CDS
TTCTTGTCGGTGGCTCGTCT+AGG 0.487944 2_4:-23195390 Msa0321660:CDS
GTGCATGATATTGTTCTTGT+CGG 0.490907 2_4:-23195403 Msa0321660:CDS
CTTCTTGTGAAAAGGCGAAA+AGG 0.494676 2_4:-23195600 Msa0321660:CDS
TTTCACTGATGCTCAAAGGT+TGG 0.498338 2_4:-23197163 Msa0321660:CDS
GGTGGAGAGAATGAAGGAAA+AGG 0.498600 2_4:-23188872 Msa0321660:CDS
TGAGAATCGTTAAAATAAGC+AGG 0.500441 2_4:+23195961 None:intergenic
TAGTGTTAAGTACAAGGGTC+AGG 0.501144 2_4:-23196091 Msa0321660:CDS
GGATGGCCACGAGGAACACT+TGG 0.501644 2_4:+23195001 None:intergenic
CGAAAAGCCACCATGGATGC+TGG 0.502548 2_4:-23195937 Msa0321660:CDS
GGTACTGGGTGTTGAGTTGG+TGG 0.504479 2_4:-23188890 Msa0321660:CDS
TGAGGTACTGGGTGTTGAGT+TGG 0.504931 2_4:-23188893 Msa0321660:CDS
ACGAGGAACACTTGGAAGAC+CGG 0.508128 2_4:+23195009 None:intergenic
GAGATTATCCACAACGATGA+AGG 0.511349 2_4:-23197213 Msa0321660:CDS
GTCTAGGATTCCAAATGTGC+AGG 0.516334 2_4:-23195374 Msa0321660:CDS
AATGAAGGAAAAGGAGTTGG+TGG 0.519691 2_4:-23188863 Msa0321660:CDS
GCCGCCAACGGTATCACCTA+TGG 0.520273 2_4:-23189091 Msa0321660:CDS
GCGAGTGACAACAACGTGCT+TGG 0.521927 2_4:-23195046 Msa0321660:CDS
AAAATTGATTCAAGAAGCTA+AGG 0.522479 2_4:-23194849 Msa0321660:CDS
TGCTTTGCTATCGATGAAAA+CGG 0.522627 2_4:-23194968 Msa0321660:CDS
TTGCTTTCACTGATGCTCAA+AGG 0.524988 2_4:-23197167 Msa0321660:CDS
ATGCTAAGAGGTTAATTGGT+AGG 0.525498 2_4:-23196197 Msa0321660:CDS
ACTGATGCTCAAAGGTTGGT+TGG 0.525931 2_4:-23197159 Msa0321660:CDS
GAGAATGAAGGAAAAGGAGT+TGG 0.526573 2_4:-23188866 Msa0321660:CDS
CGATAACCAATGACAAAGAA+AGG 0.526916 2_4:-23194892 Msa0321660:CDS
ATTTAATAGGAAGAATAAAG+TGG 0.528036 2_4:-23195662 Msa0321660:CDS
ACTGAGGTTAGATGAGGTAC+TGG 0.528808 2_4:-23188905 Msa0321660:CDS
CTCATGTGTCGCTGTTTGGT+TGG 0.529662 2_4:-23197253 Msa0321660:CDS
GCCATTGCTGGCCTTAACGT+TGG 0.532881 2_4:-23195913 Msa0321660:CDS
GAACATCTACTTCATTCCCC+TGG 0.535646 2_4:+23194642 None:intergenic
GAGGTGAGTGTTTCCAGAAG+TGG 0.537456 2_4:+23195732 None:intergenic
AAACACGAAGGGCATGTTGT+GGG 0.538509 2_4:-23197297 Msa0321660:CDS
AGCAATGGCACCAGCATCCA+TGG 0.539868 2_4:+23195927 None:intergenic
GTTGGTTGGTGGTGCTGCTA+AGG 0.541802 2_4:-23197145 Msa0321660:CDS
CACATGAGTATGTTGTTCCT+AGG 0.544545 2_4:+23197268 None:intergenic
GGTAGAGATTGGAGTTATGG+CGG 0.544665 2_4:-23188941 Msa0321660:CDS
GACGTTACCCCATTGTCACT+TGG 0.546373 2_4:-23195214 Msa0321660:intron
TCAGTGAAAGCAACAAAAGA+AGG 0.546424 2_4:+23197177 None:intergenic
TGAAGTTTATGAGGGTGAGA+GGG 0.550868 2_4:-23195074 Msa0321660:CDS
AAGGTAATGAATGCAATAGA+TGG 0.552154 2_4:-23194788 Msa0321660:CDS
AGAGGCTTATTTGGAATCAC+CGG 0.553942 2_4:-23196007 Msa0321660:CDS
ACCCTCATAAACTTCAATCA+AGG 0.556937 2_4:+23195081 None:intergenic
TCAGCAGAGGAAAGATCCAC+TGG 0.557101 2_4:-23194932 Msa0321660:CDS
GTGAATGGTGTGAAGGATGT+AGG 0.558623 2_4:-23189067 Msa0321660:CDS
TACGATTAGTGTTAAGTACA+AGG 0.559474 2_4:-23196097 Msa0321660:CDS
GATCTCATTCTTACTTCCAG+TGG 0.560329 2_4:+23194916 None:intergenic
ACCATAGGTGATACCGTTGG+CGG 0.561687 2_4:+23189090 None:intergenic
GATTGTTGAAAGTTGTCTTG+TGG 0.568361 2_4:-23195449 Msa0321660:CDS
CAATCTCCTTCATGTTTGTG+AGG 0.570747 2_4:+23196031 None:intergenic
GCGTAACTTCTTCAATGGGA+AGG 0.571597 2_4:-23195344 Msa0321660:CDS
GGAATTTCTCATCTTCAGCA+TGG 0.578307 2_4:+23194822 None:intergenic
ACTTCTGGAAACACTCACCT+CGG 0.583908 2_4:-23195730 Msa0321660:CDS
GATGCTCAAAGGTTGGTTGG+TGG 0.584104 2_4:-23197156 Msa0321660:CDS
CATGTTGTGGGCATAGACCT+AGG 0.588329 2_4:-23197285 Msa0321660:CDS
AGAGATTGGAGTTATGGCGG+AGG 0.590011 2_4:-23188938 Msa0321660:CDS
GAATAGAAATATACCAAAGA+CGG 0.590465 2_4:+23197095 None:intergenic
TTGTACTTAACACTAATCGT+AGG 0.591476 2_4:+23196099 None:intergenic
GGTATCACCTATGGTGTGAA+TGG 0.592022 2_4:-23189082 Msa0321660:CDS
CTGAAGATGAGAAATTCCTA+AGG 0.592200 2_4:-23194817 Msa0321660:CDS
GCCTTGATTGAAGTTTATGA+GGG 0.593761 2_4:-23195082 Msa0321660:CDS
ATATGCAATAGCTGCAGCAG+TGG 0.594040 2_4:+23195873 None:intergenic
GCTGAGATACCAAGTGACAA+TGG 0.594285 2_4:+23195205 None:intergenic
CCCAACGTTAAGGCCAGCAA+TGG 0.596399 2_4:+23195912 None:intergenic
TTAGGTTAGGGAAATCGAGT+TGG 0.599385 2_4:-23188962 Msa0321660:intron
CTTCACACCATTCACACCAT+AGG 0.600519 2_4:+23189075 None:intergenic
TATTGTCCTCACAAACATGA+AGG 0.601761 2_4:-23196037 Msa0321660:CDS
TATGCAATAGCTGCAGCAGT+GGG 0.602359 2_4:+23195874 None:intergenic
GACAATAGATGAGATTTCCT+CGG 0.602697 2_4:+23196053 None:intergenic
AGATGAGAAATTCCTAAGGA+AGG 0.602756 2_4:-23194813 Msa0321660:CDS
AGAATAAAGTGGATATCACT+AGG 0.603710 2_4:-23195651 Msa0321660:CDS
TTGCTTCATAAGAATAACCA+GGG 0.605023 2_4:-23194659 Msa0321660:CDS
GATAGCAAAGCATACATCCA+AGG 0.607442 2_4:+23194979 None:intergenic
ATAGAAAGCGAAGCCGCCAA+CGG 0.607943 2_4:-23189103 Msa0321660:CDS
CATGATATTGTTCTTGTCGG+TGG 0.608819 2_4:-23195400 Msa0321660:CDS
TTTGCTTCATAAGAATAACC+AGG 0.610100 2_4:-23194660 Msa0321660:CDS
AGACATCACCTTTAATTGTG+AGG 0.611395 2_4:+23195767 None:intergenic
CATCTTAACTGTTTCAGCAG+AGG 0.613572 2_4:-23194945 Msa0321660:CDS
ACACCAGCAATAACCTTGAA+CGG 0.614534 2_4:+23196132 None:intergenic
GAATGAAGTAGATGTTCTTG+AGG 0.618161 2_4:-23194636 Msa0321660:CDS
ATAGCAAAGCATACATCCAA+GGG 0.619096 2_4:+23194980 None:intergenic
TGTTCTTGAGGATCATCTGA+AGG 0.619951 2_4:-23194624 Msa0321660:CDS
TTCTCAGCGAAAAGCCACCA+TGG 0.620029 2_4:-23195944 Msa0321660:CDS
TTCTGGAAACACTCACCTCG+GGG 0.622900 2_4:-23195728 Msa0321660:CDS
TGAGTTGGTGGAGAGAATGA+AGG 0.623756 2_4:-23188878 Msa0321660:CDS
CAAGCTGCTGTGTTGAGTGA+AGG 0.627003 2_4:-23195268 Msa0321660:CDS
CAAAGCATACATCCAAGGGA+TGG 0.629656 2_4:+23194984 None:intergenic
TGATGAGGCTGTTGATTATG+GGG 0.631930 2_4:-23195299 Msa0321660:CDS
AAAACACGAAGGGCATGTTG+TGG 0.634097 2_4:-23197298 Msa0321660:CDS
CAAGTTTGAAGAAATCAACA+TGG 0.636916 2_4:-23195494 Msa0321660:CDS
CATAATCAACAGCCTCATCA+GGG 0.638573 2_4:+23195302 None:intergenic
GGAGAAGCTCCTTTATGCCG+AGG 0.641687 2_4:-23196070 Msa0321660:CDS
GATTGCTGCAATATTGATGG+AGG 0.645852 2_4:-23188814 Msa0321660:CDS
AGAAGTTCTCAACCTCCTCA+AGG 0.646239 2_4:+23195618 None:intergenic
GACAAAAGAAGTAATTGTGA+TGG 0.650690 2_4:-23195844 Msa0321660:CDS
CTAGGAATCCAAGAGCCTTG+AGG 0.655232 2_4:-23195633 Msa0321660:CDS
CTATGGTGTGAATGGTGTGA+AGG 0.656122 2_4:-23189074 Msa0321660:CDS
CTGAGGTTAGATGAGGTACT+GGG 0.657164 2_4:-23188904 Msa0321660:CDS
CAAGAGCATCAACCCTGATG+AGG 0.657948 2_4:-23195314 Msa0321660:CDS
GAAATTCCTAAGGAAGGCCA+AGG 0.662766 2_4:-23194807 Msa0321660:CDS
GGAATCCAAGAGCCTTGAGG+AGG 0.663076 2_4:-23195630 Msa0321660:CDS
GATCAATCGTGCAATTACTG+TGG 0.674376 2_4:-23194690 Msa0321660:CDS
ACGATTAGTGTTAAGTACAA+GGG 0.676520 2_4:-23196096 Msa0321660:CDS
GAAGGATGTAGGAACGATTG+GGG 0.678493 2_4:-23189056 Msa0321660:CDS
CACACCATAGGTGATACCGT+TGG 0.682773 2_4:+23189087 None:intergenic
GGTTGAACTGAGGTTAGATG+AGG 0.684768 2_4:-23188911 Msa0321660:CDS
AAACATGAAGGAGATTGCAG+AGG 0.695775 2_4:-23196025 Msa0321660:CDS
ACATCCAAGGGATGGCCACG+AGG 0.704531 2_4:+23194992 None:intergenic
CGCATGGCGAGAAAACACGA+AGG 0.705045 2_4:-23197309 Msa0321660:CDS
GGTCTTCCAAGTGTTCCTCG+TGG 0.707924 2_4:-23195007 Msa0321660:CDS
GCATGGCGAGAAAACACGAA+GGG 0.708265 2_4:-23197308 Msa0321660:CDS
TGAGATACCAAGTGACAATG+GGG 0.709556 2_4:+23195207 None:intergenic
TGCTTCATAAGAATAACCAG+GGG 0.713870 2_4:-23194658 Msa0321660:CDS
TCTGGAAACACTCACCTCGG+GGG 0.720115 2_4:-23195727 Msa0321660:CDS
AATGGCACCAGCATCCATGG+TGG 0.736459 2_4:+23195930 None:intergenic
CGGAGGATGAGGTTGAACTG+AGG 0.737985 2_4:-23188921 Msa0321660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAACACGAAGGGCATGTTG+TGG - chr2_4:23188818-23188837 Msa0321660:CDS 45.0%
! AAACACGAAGGGCATGTTGT+GGG - chr2_4:23188819-23188838 Msa0321660:CDS 45.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 23188810 23197328 23188810 ID=Msa0321660;Name=Msa0321660
chr2_4 mRNA 23188810 23197328 23188810 ID=Msa0321660-mRNA-1;Parent=Msa0321660;Name=Msa0321660-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0|0|0.6|1|1|5|0|658
chr2_4 exon 23197109 23197328 23197109 ID=Msa0321660-mRNA-1:exon:8545;Parent=Msa0321660-mRNA-1
chr2_4 exon 23195215 23196219 23195215 ID=Msa0321660-mRNA-1:exon:8544;Parent=Msa0321660-mRNA-1
chr2_4 exon 23194615 23195118 23194615 ID=Msa0321660-mRNA-1:exon:8543;Parent=Msa0321660-mRNA-1
chr2_4 exon 23189054 23189130 23189054 ID=Msa0321660-mRNA-1:exon:8542;Parent=Msa0321660-mRNA-1
chr2_4 exon 23188810 23188980 23188810 ID=Msa0321660-mRNA-1:exon:8541;Parent=Msa0321660-mRNA-1
chr2_4 CDS 23197109 23197328 23197109 ID=Msa0321660-mRNA-1:cds;Parent=Msa0321660-mRNA-1
chr2_4 CDS 23195215 23196219 23195215 ID=Msa0321660-mRNA-1:cds;Parent=Msa0321660-mRNA-1
chr2_4 CDS 23194615 23195118 23194615 ID=Msa0321660-mRNA-1:cds;Parent=Msa0321660-mRNA-1
chr2_4 CDS 23189054 23189130 23189054 ID=Msa0321660-mRNA-1:cds;Parent=Msa0321660-mRNA-1
chr2_4 CDS 23188810 23188980 23188810 ID=Msa0321660-mRNA-1:cds;Parent=Msa0321660-mRNA-1
Gene Sequence

>Msa0321660

ATGGCGAGAAAACACGAAGGGCATGTTGTGGGCATAGACCTAGGAACAACATACTCATGTGTCGCTGTTTGGTTGGAAGAAAAAAACAGAGTTGAGATTATCCACAACGATGAAGGCAACAAAATTACACCTTCTTTTGTTGCTTTCACTGATGCTCAAAGGTTGGTTGGTGGTGCTGCTAAGGATCAAGCTGCTATCAACTCACAAAACACCGTCTTTGATGCTAAGAGGTTAATTGGTAGGAAATTTAGTGACCCCGTTGTTCAAAAAGATATATTGTTGTGGCCGTTCAAGGTTATTGCTGGTGTTAATGACAAACCTACGATTAGTGTTAAGTACAAGGGTCAGGAGAAGCTCCTTTATGCCGAGGAAATCTCATCTATTGTCCTCACAAACATGAAGGAGATTGCAGAGGCTTATTTGGAATCACCGGTTAAAAATGCAGTTGTCACTGTTCCTGCTTATTTTAACGATTCTCAGCGAAAAGCCACCATGGATGCTGGTGCCATTGCTGGCCTTAACGTTGGGAAGATAATCAATGAACCCACTGCTGCAGCTATTGCATATGGCCTTGACAAAAGAAGTAATTGTGATGGTGAAAGAAATATTTTTGTCTTTGATCTTGGTGGTGGGACTTTTGATGTGTCTCTCCTCACAATTAAAGGTGATGTCTTTGAAGTTAAAGCCACTTCTGGAAACACTCACCTCGGGGGAGAGGACTTTGATAACAGAATGTTGAACTACTTTGTGCAAGAATTTAATAGGAAGAATAAAGTGGATATCACTAGGAATCCAAGAGCCTTGAGGAGGTTGAGAACTTCTTGTGAAAAGGCGAAAAGGACACTCTCTTTCAAATTGGTCACCACTATTGAGATTGATTATTTATATGAGGACATTGACTTCTCTACATCAATCACTCGTGTCAAGTTTGAAGAAATCAACATGGACCTTTTTAATGAGTGTATGAAGATTGTTGAAAGTTGTCTTGTGGATTCAAAGATCGACAAGTGCAGTGTGCATGATATTGTTCTTGTCGGTGGCTCGTCTAGGATTCCAAATGTGCAGGAACTATTGCGTAACTTCTTCAATGGGAAGGAGTTGTGCAAGAGCATCAACCCTGATGAGGCTGTTGATTATGGGGCAGCTGTGCAAGCTGCTGTGTTGAGTGAAGGCATTAAGAATGTTCCAAACTTGGTGCTGCGAGACGTTACCCCATTGTCACTTGATAACCAATATAGTGCCTTGATTGAAGTTTATGAGGGTGAGAGGGCAAGAGCGAGTGACAACAACGTGCTTGGTTTTTTCAATCTTTCCGGTCTTCCAAGTGTTCCTCGTGGCCATCCCTTGGATGTATGCTTTGCTATCGATGAAAACGGCATCTTAACTGTTTCAGCAGAGGAAAGATCCACTGGAAGTAAGAATGAGATCACGATAACCAATGACAAAGAAAGGTTATCCAGAGTCGAAATTGAAAAATTGATTCAAGAAGCTAAGGAGTACCATGCTGAAGATGAGAAATTCCTAAGGAAGGCCAAGGTAATGAATGCAATAGATGGCTGCATTGACAAGTTGAAGAATGCTCTAAAGAGTAAGGATGTCAAACTAAAGCTTTCTTATCAAAAAAATAAAAAGATCAATCGTGCAATTACTGTGGCTAAAGATTTGCTTCATAAGAATAACCAGGGGAATGAAGTAGATGTTCTTGAGGATCATCTGAAGGAGCTTGAGAAATTGATAGAAAGCGAAGCCGCCAACGGTATCACCTATGGTGTGAATGGTGTGAAGGATGTAGGAACGATTGGGGATGTTAGGGAAATCGAGTTGGTAGAGATTGGAGTTATGGCGGAGGATGAGGTTGAACTGAGGTTAGATGAGGTACTGGGTGTTGAGTTGGTGGAGAGAATGAAGGAAAAGGAGTTGGTGGTTGAGGAATGGGTTGTGGTAGTTTATGATTGCTGCAATATTGATGGAGGTTAG
Protein sequence

>Msa0321660

MARKHEGHVVGIDLGTTYSCVAVWLEEKNRVEIIHNDEGNKITPSFVAFTDAQRLVGGAAKDQAAINSQNTVFDAKRLIGRKFSDPVVQKDILLWPFKVIAGVNDKPTISVKYKGQEKLLYAEEISSIVLTNMKEIAEAYLESPVKNAVVTVPAYFNDSQRKATMDAGAIAGLNVGKIINEPTAAAIAYGLDKRSNCDGERNIFVFDLGGGTFDVSLLTIKGDVFEVKATSGNTHLGGEDFDNRMLNYFVQEFNRKNKVDITRNPRALRRLRTSCEKAKRTLSFKLVTTIEIDYLYEDIDFSTSITRVKFEEINMDLFNECMKIVESCLVDSKIDKCSVHDIVLVGGSSRIPNVQELLRNFFNGKELCKSINPDEAVDYGAAVQAAVLSEGIKNVPNLVLRDVTPLSLDNQYSALIEVYEGERARASDNNVLGFFNLSGLPSVPRGHPLDVCFAIDENGILTVSAEERSTGSKNEITITNDKERLSRVEIEKLIQEAKEYHAEDEKFLRKAKVMNAIDGCIDKLKNALKSKDVKLKLSYQKNKKINRAITVAKDLLHKNNQGNEVDVLEDHLKELEKLIESEAANGITYGVNGVKDVGTIGDVREIELVEIGVMAEDEVELRLDEVLGVELVERMKEKELVVEEWVVVVYDCCNIDGG*