AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0325840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0325840 MtrunA17_Chr2g0304251 85.714 84 12 0 14 97 73 156 2.31e-46 155
Msa0325840 MtrunA17_Chr4g0064851 60.465 86 32 1 14 97 75 160 2.87e-31 114
Msa0325840 MtrunA17_Chr4g0061271 46.316 95 50 1 4 97 50 144 2.97e-24 95.1
Msa0325840 MtrunA17_Chr5g0418671 49.398 83 42 0 15 97 59 141 3.16e-24 94.7
Msa0325840 MtrunA17_Chr3g0086291 45.455 88 46 1 12 97 59 146 2.00e-22 89.7
Msa0325840 MtrunA17_Chr3g0086271 46.429 84 45 0 14 97 61 144 2.08e-22 89.7
Msa0325840 MtrunA17_Chr3g0086251 46.429 84 45 0 14 97 170 253 3.88e-22 89.0
Msa0325840 MtrunA17_Chr3g0086241 45.455 88 46 1 12 97 59 146 1.01e-21 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0325840 AT5G34940.2 57.143 84 36 0 14 97 67 150 2.59e-31 115
Msa0325840 AT5G34940.3 50.000 84 42 0 14 97 67 150 3.08e-26 99.8
Msa0325840 AT5G07830.1 48.193 83 43 0 15 97 67 149 5.16e-24 94.7
Msa0325840 AT5G61250.3 44.578 83 46 0 15 97 64 146 1.85e-22 90.5
Msa0325840 AT5G61250.1 44.578 83 46 0 15 97 64 146 1.85e-22 90.5
Msa0325840 AT5G61250.2 44.578 83 46 0 15 97 64 146 1.85e-22 90.5

Find 85 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGATGCAACGTGGTTTA+AGG 0.142167 2_4:-33946457 Msa0325840:three_prime_UTR
TTTGGATTGAATGCCCTTTA+TGG 0.158147 2_4:-33946847 Msa0325840:three_prime_UTR
GATTATGTCTATATACTATT+TGG 0.187068 2_4:-33948468 Msa0325840:CDS
TTGTATCATTAACAGGTATT+TGG 0.205946 2_4:-33945691 Msa0325840:intron
TGACTAATGGATTCGCCTTC+TGG 0.215158 2_4:-33946935 Msa0325840:three_prime_UTR
ATGCATTTGTGTATAGTTTC+TGG 0.217121 2_4:-33945951 Msa0325840:intron
ATTGTTAAACTGTTTATGTT+AGG 0.260046 2_4:-33946882 Msa0325840:three_prime_UTR
TTGCAAGATGAACTGGTATA+TGG 0.282033 2_4:-33947152 Msa0325840:CDS
TGATTCTGGAAATGGGTTCT+TGG 0.346192 2_4:-33948496 None:intergenic
GTCTGGATTGGGAAGGAAAT+TGG 0.347707 2_4:+33945581 None:intergenic
TATTGCAGACAGAGTTTGAT+AGG 0.355616 2_4:-33945630 Msa0325840:three_prime_UTR
AGAAACACATCTGCAATGTT+TGG 0.370641 2_4:-33947092 Msa0325840:CDS
TATGGAAAGTCGGTACATAA+TGG 0.393891 2_4:-33946829 Msa0325840:three_prime_UTR
ACAGCATGGGTTGGTGAGTC+AGG 0.398551 2_4:-33946009 Msa0325840:three_prime_UTR
ATGTACCGACTTTCCATAAA+GGG 0.407723 2_4:+33946834 None:intergenic
GCAGATGTGTTTCTAACAAA+AGG 0.408452 2_4:+33947101 None:intergenic
CCTCCAGGCGCAATGACTAA+TGG 0.413718 2_4:+33946483 None:intergenic
TTGTTAAACTGTTTATGTTA+GGG 0.418016 2_4:-33946881 Msa0325840:three_prime_UTR
GAAATTCATGGATGGGAACT+TGG 0.427992 2_4:-33946736 Msa0325840:intron
AGTTTGATAGGAGGAAACTA+TGG 0.431640 2_4:-33945618 Msa0325840:three_prime_UTR
TCACCATTGAAACTGAGATT+AGG 0.436550 2_4:-33947182 Msa0325840:CDS
TACCTATAGTAGTCTGGATT+GGG 0.447932 2_4:+33945570 None:intergenic
ATTTAATACCTATAGTAGTC+TGG 0.451269 2_4:+33945564 None:intergenic
ACACACCACATTTATAGCCT+TGG 0.452398 2_4:-33946396 Msa0325840:three_prime_UTR
ATACCTATAGTAGTCTGGAT+TGG 0.458022 2_4:+33945569 None:intergenic
TCTCAGTTTCAATGGTGAAA+AGG 0.460420 2_4:+33947187 None:intergenic
TGGCAGTTTGCAAGATGAAC+TGG 0.463605 2_4:-33947159 Msa0325840:CDS
AGTTCATCCCATCTTGGCAT+TGG 0.469463 2_4:+33947050 None:intergenic
TGCAAGATGAACTGGTATAT+GGG 0.470523 2_4:-33947151 Msa0325840:CDS
TCGTTTATTCAATACACTGT+TGG 0.471428 2_4:-33946769 Msa0325840:three_prime_UTR
TGATTAAGTTCATCCCATCT+TGG 0.471533 2_4:+33947044 None:intergenic
TTCCCAATCCAGACTACTAT+AGG 0.475408 2_4:-33945572 None:intergenic
ATCTAATAAATCTGTGGATG+TGG 0.485447 2_4:-33946421 Msa0325840:three_prime_UTR
CCACCTAATCTCAGTTTCAA+TGG 0.488877 2_4:+33947179 None:intergenic
TTAAGAAAATCTTGTTGTTG+AGG 0.489628 2_4:+33947343 None:intergenic
TATGTACCGACTTTCCATAA+AGG 0.490448 2_4:+33946833 None:intergenic
TTGAATAAACGATTCGGCAT+TGG 0.492524 2_4:+33946780 None:intergenic
GGAAAGAACTATGAAATTCA+TGG 0.493133 2_4:-33946748 Msa0325840:three_prime_UTR
CATCCCATCTTGGCATTGGA+AGG 0.497102 2_4:+33947054 None:intergenic
CATGGGTTGGTGAGTCAGGA+GGG 0.509443 2_4:-33946005 Msa0325840:three_prime_UTR
AGTGTATTGAATAAACGATT+CGG 0.509792 2_4:+33946774 None:intergenic
GGTGCCTTCCAATGCCAAGA+TGG 0.519720 2_4:-33947058 Msa0325840:CDS
TGTCTATATACTATTTGGCA+TGG 0.521164 2_4:-33948463 Msa0325840:CDS
AGAGACAAAACTACATTCAA+AGG 0.521273 2_4:-33946069 Msa0325840:three_prime_UTR
AGAACTATGAAATTCATGGA+TGG 0.527137 2_4:-33946744 Msa0325840:three_prime_UTR
GTATGCACTCGCGTGAGCGC+AGG 0.529697 2_4:-33946977 Msa0325840:three_prime_UTR
AAGCCATTAGTCATTGCGCC+TGG 0.532443 2_4:-33946486 Msa0325840:three_prime_UTR
TTTCTAACAAAAGGAGTGCA+TGG 0.533788 2_4:+33947110 None:intergenic
GAACTATGAAATTCATGGAT+GGG 0.542759 2_4:-33946743 Msa0325840:three_prime_UTR
GTTGCATCAAAGAAACCTCC+AGG 0.545777 2_4:+33946468 None:intergenic
GGCGCAATGACTAATGGCTT+AGG 0.552752 2_4:+33946489 None:intergenic
GTGCCTTCCAATGCCAAGAT+GGG 0.557147 2_4:-33947057 Msa0325840:CDS
TGGCAGGCAATGAATTGAGT+GGG 0.557347 2_4:-33946605 Msa0325840:intron
TTGTCTCTATCAAGATAGGA+AGG 0.557907 2_4:+33946084 None:intergenic
TCGGCATTGGTGTAGTTCCA+AGG 0.562201 2_4:+33946793 None:intergenic
CACATTTATAGCCTTGGACC+AGG 0.565926 2_4:-33946390 Msa0325840:intron
GCATGGGTTGGTGAGTCAGG+AGG 0.569855 2_4:-33946006 Msa0325840:three_prime_UTR
ATGGGTTGGTGAGTCAGGAG+GGG 0.574317 2_4:-33946004 Msa0325840:three_prime_UTR
ACCAACCCATGCTGTTGCTG+AGG 0.580019 2_4:+33946017 None:intergenic
CAGGAACCTCAGCAACAGCA+TGG 0.580368 2_4:-33946023 Msa0325840:three_prime_UTR
ACCTCAGCAACAGCATGGGT+TGG 0.582674 2_4:-33946018 Msa0325840:three_prime_UTR
CCATTGAAACTGAGATTAGG+TGG 0.583219 2_4:-33947179 Msa0325840:CDS
ATGGTTATACAGAAGGACCT+TGG 0.589347 2_4:-33946810 Msa0325840:three_prime_UTR
ATGCATCGGATACAAGATGA+TGG 0.592109 2_4:+33945968 None:intergenic
GGCGAAAAGTACCTGGTCCA+AGG 0.595921 2_4:+33946379 None:intergenic
GCAATGTTTGGATTCTCACA+AGG 0.596531 2_4:-33947080 Msa0325840:CDS
TGGATTGGGAAGGAAATTGG+TGG 0.602050 2_4:+33945584 None:intergenic
GAATGCCCTTTATGGAAAGT+CGG 0.602404 2_4:-33946839 Msa0325840:three_prime_UTR
GTACATAATGGTTATACAGA+AGG 0.609586 2_4:-33946817 Msa0325840:three_prime_UTR
AAGCAAATCTAATAAATCTG+TGG 0.613723 2_4:-33946427 Msa0325840:three_prime_UTR
CTGGCAGGCAATGAATTGAG+TGG 0.614470 2_4:-33946606 Msa0325840:intron
GAAACTATACACAAATGCAT+CGG 0.619169 2_4:+33945954 None:intergenic
GCATAGTTTACAAGTCCAGA+AGG 0.620597 2_4:+33946920 None:intergenic
TGCAGACAGAGTTTGATAGG+AGG 0.621831 2_4:-33945627 Msa0325840:three_prime_UTR
AACAGGTATTTGGATCAACT+CGG 0.623593 2_4:-33945681 Msa0325840:intron
AGGAGTTGATACGCACCTTG+TGG 0.627565 2_4:-33946121 Msa0325840:three_prime_UTR
CCATTAGTCATTGCGCCTGG+AGG 0.629727 2_4:-33946483 Msa0325840:three_prime_UTR
AGGAACCTCAGCAACAGCAT+GGG 0.631168 2_4:-33946022 Msa0325840:three_prime_UTR
CTGGTCCAAGGCTATAAATG+TGG 0.631236 2_4:+33946391 None:intergenic
CAATGTTTGGATTCTCACAA+GGG 0.640318 2_4:-33947079 Msa0325840:CDS
TATAGTAGTCTGGATTGGGA+AGG 0.650930 2_4:+33945574 None:intergenic
GGCAGGCAATGAATTGAGTG+GGG 0.653685 2_4:-33946604 Msa0325840:intron
GAGGTTTCTTTGATGCAACG+TGG 0.664358 2_4:-33946464 Msa0325840:three_prime_UTR
TCAGGAGGGGCTTATAACAG+TGG 0.681174 2_4:-33945991 Msa0325840:three_prime_UTR
GCAGGCAATGAATTGAGTGG+GGG 0.733139 2_4:-33946603 Msa0325840:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAAGATAAAGAAAA+AGG + chr2_4:33945834-33945853 None:intergenic 10.0%
!! TTATTAATTAATTAACTTCT+TGG - chr2_4:33946378-33946397 Msa0325840:intron 10.0%
!! CTATTAATCAATAAATTAAA+TGG + chr2_4:33946609-33946628 None:intergenic 10.0%
!!! TATTGTTATTGTGATAAAAA+TGG - chr2_4:33946775-33946794 Msa0325840:three_prime_UTR 15.0%
!! GATTATGTCTATATACTATT+TGG - chr2_4:33945591-33945610 Msa0325840:three_prime_UTR 20.0%
!! AAAGATAAAGAAAAAGGAAA+AGG + chr2_4:33945828-33945847 None:intergenic 20.0%
!! ATTGTTAAACTGTTTATGTT+AGG - chr2_4:33947177-33947196 Msa0325840:CDS 20.0%
!! TTGTTAAACTGTTTATGTTA+GGG - chr2_4:33947178-33947197 Msa0325840:CDS 20.0%
!!! AGAAATAATTGATGATTTGA+AGG + chr2_4:33947894-33947913 None:intergenic 20.0%
!!! TACTTTCAATAACATTTTTC+AGG + chr2_4:33948016-33948035 None:intergenic 20.0%
!!! AAAAATGTTATTGAAAGTAC+AGG - chr2_4:33948017-33948036 Msa0325840:intron 20.0%
!!! TGATTTTATTTGTGCAACTT+TGG - chr2_4:33945726-33945745 Msa0325840:intron 25.0%
! TGTTTGTCTTGAAATCTTTA+AGG - chr2_4:33945860-33945879 Msa0325840:intron 25.0%
!!! TTTTTATTTGCAGATTAGCT+TGG + chr2_4:33946177-33946196 None:intergenic 25.0%
!!! TCTTGTTTTCTACAAACAAT+GGG + chr2_4:33946253-33946272 None:intergenic 25.0%
!!! ATCTTGTTTTCTACAAACAA+TGG + chr2_4:33946254-33946273 None:intergenic 25.0%
!!! AAATCTTGTGCATTTTTTCA+AGG - chr2_4:33946651-33946670 Msa0325840:intron 25.0%
!!! TTAAGAAAATCTTGTTGTTG+AGG + chr2_4:33946719-33946738 None:intergenic 25.0%
!! ATTTTCTTAAATGCAGTGAA+AGG - chr2_4:33946729-33946748 Msa0325840:intron 25.0%
! TATCACAATAACAATAGAGA+TGG + chr2_4:33946771-33946790 None:intergenic 25.0%
! AATCATTTCTTCCAAAAATC+AGG - chr2_4:33947033-33947052 Msa0325840:CDS 25.0%
! ATCATTTCTTCCAAAAATCA+GGG - chr2_4:33947034-33947053 Msa0325840:CDS 25.0%
! AGTGTATTGAATAAACGATT+CGG + chr2_4:33947288-33947307 None:intergenic 25.0%
! AATAAAATTCTTCTTGCCTA+AGG - chr2_4:33947398-33947417 Msa0325840:intron 25.0%
! AAGCAAATCTAATAAATCTG+TGG - chr2_4:33947632-33947651 Msa0325840:intron 25.0%
! TGTATGATTGTATCATTAAC+AGG - chr2_4:33948361-33948380 Msa0325840:intron 25.0%
! TTGTATCATTAACAGGTATT+TGG - chr2_4:33948368-33948387 Msa0325840:intron 25.0%
TGTCTATATACTATTTGGCA+TGG - chr2_4:33945596-33945615 Msa0325840:three_prime_UTR 30.0%
! GTTATGATGATGTGAAGAAA+GGG - chr2_4:33945659-33945678 Msa0325840:three_prime_UTR 30.0%
AAAGGGAATGTTATGATTGA+TGG - chr2_4:33945676-33945695 Msa0325840:three_prime_UTR 30.0%
TTATTTGTGCAACTTTGGAT+TGG - chr2_4:33945731-33945750 Msa0325840:intron 30.0%
ACACTTGTAATTACATGTGT+CGG + chr2_4:33945962-33945981 None:intergenic 30.0%
ACACATGTAATTACAAGTGT+CGG - chr2_4:33945962-33945981 Msa0325840:three_prime_UTR 30.0%
!! TGTTTTCGAAATGTTGTTTG+AGG - chr2_4:33946130-33946149 Msa0325840:intron 30.0%
!!! CAGAACATTGTTGCTTTTTA+TGG + chr2_4:33946203-33946222 None:intergenic 30.0%
! ATAATAATCCTGTTTTCTGC+AGG - chr2_4:33946340-33946359 Msa0325840:intron 30.0%
! TAATAATCCTGTTTTCTGCA+GGG - chr2_4:33946341-33946360 Msa0325840:intron 30.0%
!! TAATTTTGATCTATGAAGCG+CGG - chr2_4:33946410-33946429 Msa0325840:three_prime_UTR 30.0%
TTCAGTGTAATTACAAGTGT+CGG - chr2_4:33946488-33946507 Msa0325840:three_prime_UTR 30.0%
! CCAAATTTTACCCTGATTTT+TGG + chr2_4:33947047-33947066 None:intergenic 30.0%
CCAAAAATCAGGGTAAAATT+TGG - chr2_4:33947044-33947063 Msa0325840:CDS 30.0%
! GTTAGGGCTAAGATTATTTT+TGG - chr2_4:33947194-33947213 Msa0325840:intron 30.0%
GTACATAATGGTTATACAGA+AGG - chr2_4:33947242-33947261 Msa0325840:intron 30.0%
TCGTTTATTCAATACACTGT+TGG - chr2_4:33947290-33947309 Msa0325840:intron 30.0%
GGAAAGAACTATGAAATTCA+TGG - chr2_4:33947311-33947330 Msa0325840:intron 30.0%
AGAACTATGAAATTCATGGA+TGG - chr2_4:33947315-33947334 Msa0325840:intron 30.0%
GAACTATGAAATTCATGGAT+GGG - chr2_4:33947316-33947335 Msa0325840:intron 30.0%
ATCTAATAAATCTGTGGATG+TGG - chr2_4:33947638-33947657 Msa0325840:intron 30.0%
GTAATGAATATCAGCCAATT+CGG + chr2_4:33947704-33947723 None:intergenic 30.0%
CAGTGTTAAACTACTTTACA+CGG + chr2_4:33947778-33947797 None:intergenic 30.0%
!!! AAGTTCTTTATTTTGCCACA+TGG + chr2_4:33947826-33947845 None:intergenic 30.0%
!! ACTTAATTTGTCTTCTACAC+AGG - chr2_4:33947918-33947937 Msa0325840:intron 30.0%
!!! GATTCAGAATTTTTTCCACA+AGG + chr2_4:33947956-33947975 None:intergenic 30.0%
!! AGTTTTGTCTCTATCAAGAT+AGG + chr2_4:33947982-33948001 None:intergenic 30.0%
AGAGACAAAACTACATTCAA+AGG - chr2_4:33947990-33948009 Msa0325840:intron 30.0%
GAAACTATACACAAATGCAT+CGG + chr2_4:33948108-33948127 None:intergenic 30.0%
ATGCATTTGTGTATAGTTTC+TGG - chr2_4:33948108-33948127 Msa0325840:intron 30.0%
TCCTTGATTTAGACTCATTT+AGG - chr2_4:33948151-33948170 Msa0325840:intron 30.0%
CCTTGATTTAGACTCATTTA+GGG - chr2_4:33948152-33948171 Msa0325840:intron 30.0%
CTTGATTTAGACTCATTTAG+GGG - chr2_4:33948153-33948172 Msa0325840:intron 30.0%
TTGAATGTAACCACATGTAT+TGG + chr2_4:33948252-33948271 None:intergenic 30.0%
! GGTTATGATGATGTGAAGAA+AGG - chr2_4:33945658-33945677 Msa0325840:three_prime_UTR 35.0%
! ATTGATGGTAGTTCTGTGAT+TGG - chr2_4:33945691-33945710 Msa0325840:intron 35.0%
GTACCATAATCACATTTCTG+AGG + chr2_4:33945763-33945782 None:intergenic 35.0%
TGATTATGGTACATGTAGCT+GGG - chr2_4:33945771-33945790 Msa0325840:intron 35.0%
!! TGAGTTCTAGAATGTTTGCT+TGG - chr2_4:33945883-33945902 Msa0325840:intron 35.0%
CATTCTATGAAGCACAGATA+TGG - chr2_4:33945914-33945933 Msa0325840:intron 35.0%
! TTTGAGGTTTGAATGCATGT+TGG - chr2_4:33946146-33946165 Msa0325840:intron 35.0%
TTGAGGTTTGAATGCATGTT+GGG - chr2_4:33946147-33946166 Msa0325840:intron 35.0%
AGATCAAAACCTATGAAGCA+CGG + chr2_4:33946577-33946596 None:intergenic 35.0%
!!! TTTTTTTTTTCATCTCGCGC+AGG - chr2_4:33946692-33946711 Msa0325840:intron 35.0%
TCTCAGTTTCAATGGTGAAA+AGG + chr2_4:33946875-33946894 None:intergenic 35.0%
TCACCATTGAAACTGAGATT+AGG - chr2_4:33946877-33946896 Msa0325840:three_prime_UTR 35.0%
TTGCAAGATGAACTGGTATA+TGG - chr2_4:33946907-33946926 Msa0325840:three_prime_UTR 35.0%
TGCAAGATGAACTGGTATAT+GGG - chr2_4:33946908-33946927 Msa0325840:three_prime_UTR 35.0%
! TTTCTAACAAAAGGAGTGCA+TGG + chr2_4:33946952-33946971 None:intergenic 35.0%
! GCAGATGTGTTTCTAACAAA+AGG + chr2_4:33946961-33946980 None:intergenic 35.0%
AGAAACACATCTGCAATGTT+TGG - chr2_4:33946967-33946986 Msa0325840:three_prime_UTR 35.0%
CAATGTTTGGATTCTCACAA+GGG - chr2_4:33946980-33946999 Msa0325840:three_prime_UTR 35.0%
TGATTAAGTTCATCCCATCT+TGG + chr2_4:33947018-33947037 None:intergenic 35.0%
TTTGGATTGAATGCCCTTTA+TGG - chr2_4:33947212-33947231 Msa0325840:intron 35.0%
!! ATGTACCGACTTTCCATAAA+GGG + chr2_4:33947228-33947247 None:intergenic 35.0%
!! TATGTACCGACTTTCCATAA+AGG + chr2_4:33947229-33947248 None:intergenic 35.0%
TATGGAAAGTCGGTACATAA+TGG - chr2_4:33947230-33947249 Msa0325840:intron 35.0%
! TTGAATAAACGATTCGGCAT+TGG + chr2_4:33947282-33947301 None:intergenic 35.0%
! TTAGTCCTCTATTTTCCATG+TGG - chr2_4:33947808-33947827 Msa0325840:intron 35.0%
! TTTTGCCACATGGAAAATAG+AGG + chr2_4:33947816-33947835 None:intergenic 35.0%
TTGTCTCTATCAAGATAGGA+AGG + chr2_4:33947978-33947997 None:intergenic 35.0%
GAGTCTAAATCAAGGATCAA+GGG + chr2_4:33948147-33948166 None:intergenic 35.0%
TGAGTCTAAATCAAGGATCA+AGG + chr2_4:33948148-33948167 None:intergenic 35.0%
CCCTAAATGAGTCTAAATCA+AGG + chr2_4:33948155-33948174 None:intergenic 35.0%
! ATGCCTTCAATCTAAAGTGT+CGG + chr2_4:33948198-33948217 None:intergenic 35.0%
! ACTTTAGATTGAAGGCATGT+CGG - chr2_4:33948200-33948219 Msa0325840:intron 35.0%
AACAGGTATTTGGATCAACT+CGG - chr2_4:33948378-33948397 Msa0325840:intron 35.0%
TATTGCAGACAGAGTTTGAT+AGG - chr2_4:33948429-33948448 Msa0325840:intron 35.0%
AGTTTGATAGGAGGAAACTA+TGG - chr2_4:33948441-33948460 Msa0325840:CDS 35.0%
AACACAGTGAGTGATGAAAG+TGG - chr2_4:33945637-33945656 Msa0325840:three_prime_UTR 40.0%
! TTTGTGCAACTTTGGATTGG+TGG - chr2_4:33945734-33945753 Msa0325840:intron 40.0%
! CCATAATCACATTTCTGAGG+AGG + chr2_4:33945760-33945779 None:intergenic 40.0%
CCTCCTCAGAAATGTGATTA+TGG - chr2_4:33945757-33945776 Msa0325840:intron 40.0%
GTGATTATGGTACATGTAGC+TGG - chr2_4:33945770-33945789 Msa0325840:intron 40.0%
GATTATGGTACATGTAGCTG+GGG - chr2_4:33945772-33945791 Msa0325840:intron 40.0%
TCTTGCTTCTCTTCTCAATC+TGG - chr2_4:33945795-33945814 Msa0325840:intron 40.0%
CAATAAACCCTGCAGAAAAC+AGG + chr2_4:33946351-33946370 None:intergenic 40.0%
GTCGACACTGCTAATAATCT+GGG - chr2_4:33946449-33946468 Msa0325840:three_prime_UTR 40.0%
TCGACACTGCTAATAATCTG+GGG - chr2_4:33946450-33946469 Msa0325840:three_prime_UTR 40.0%
CCACCTAATCTCAGTTTCAA+TGG + chr2_4:33946883-33946902 None:intergenic 40.0%
CCATTGAAACTGAGATTAGG+TGG - chr2_4:33946880-33946899 Msa0325840:three_prime_UTR 40.0%
GCAATGTTTGGATTCTCACA+AGG - chr2_4:33946979-33946998 Msa0325840:three_prime_UTR 40.0%
! CGTGAGTGTTTTGTGACTAA+TGG - chr2_4:33947111-33947130 Msa0325840:CDS 40.0%
GCATAGTTTACAAGTCCAGA+AGG + chr2_4:33947142-33947161 None:intergenic 40.0%
GAATGCCCTTTATGGAAAGT+CGG - chr2_4:33947220-33947239 Msa0325840:intron 40.0%
ATGGTTATACAGAAGGACCT+TGG - chr2_4:33947249-33947268 Msa0325840:intron 40.0%
GAAATTCATGGATGGGAACT+TGG - chr2_4:33947323-33947342 Msa0325840:intron 40.0%
ACTCAATGTACTCAAGCCTT+AGG + chr2_4:33947417-33947436 None:intergenic 40.0%
CTTTGATGCAACGTGGTTTA+AGG - chr2_4:33947602-33947621 Msa0325840:intron 40.0%
ACACACCACATTTATAGCCT+TGG - chr2_4:33947663-33947682 Msa0325840:intron 40.0%
ATGCATCGGATACAAGATGA+TGG + chr2_4:33948094-33948113 None:intergenic 40.0%
ACACCGACACTTTAGATTGA+AGG - chr2_4:33948192-33948211 Msa0325840:intron 40.0%
GTATCTAACGCCAATACATG+TGG - chr2_4:33948239-33948258 Msa0325840:intron 40.0%
! TATAGTAGTCTGGATTGGGA+AGG + chr2_4:33948488-33948507 None:intergenic 40.0%
TATGAAGCACGTACACTCCT+TGG + chr2_4:33946063-33946082 None:intergenic 45.0%
TCTGTTCTGTCACTGTTGTG+TGG + chr2_4:33946290-33946309 None:intergenic 45.0%
CGTCGACACTGCTAATAATC+TGG - chr2_4:33946448-33946467 Msa0325840:three_prime_UTR 45.0%
TGGCAGTTTGCAAGATGAAC+TGG - chr2_4:33946900-33946919 Msa0325840:three_prime_UTR 45.0%
AGTTCATCCCATCTTGGCAT+TGG + chr2_4:33947012-33947031 None:intergenic 45.0%
!! TGACTAATGGATTCGCCTTC+TGG - chr2_4:33947124-33947143 Msa0325840:CDS 45.0%
ATTCATTGCCTGCCAGTGAA+AGG + chr2_4:33947449-33947468 None:intergenic 45.0%
TGGCAGGCAATGAATTGAGT+GGG - chr2_4:33947454-33947473 Msa0325840:intron 45.0%
GTTGCATCAAAGAAACCTCC+AGG + chr2_4:33947594-33947613 None:intergenic 45.0%
GAGGTTTCTTTGATGCAACG+TGG - chr2_4:33947595-33947614 Msa0325840:intron 45.0%
CTGGTCCAAGGCTATAAATG+TGG + chr2_4:33947671-33947690 None:intergenic 45.0%
! CACATTTATAGCCTTGGACC+AGG - chr2_4:33947669-33947688 Msa0325840:intron 45.0%
!! ATCGGTGTTGTTTCCAGTGT+CGG - chr2_4:33948295-33948314 Msa0325840:intron 45.0%
TGCAGACAGAGTTTGATAGG+AGG - chr2_4:33948432-33948451 Msa0325840:intron 45.0%
! TGGATTGGGAAGGAAATTGG+TGG + chr2_4:33948478-33948497 None:intergenic 45.0%
! GTCTGGATTGGGAAGGAAAT+TGG + chr2_4:33948481-33948500 None:intergenic 45.0%
!!! TTCATTTTTAAAATTATTAT+CGG - chr2_4:33948277-33948296 Msa0325840:intron 5.0%
GCACAGATATGGACACGACA+TGG - chr2_4:33945925-33945944 Msa0325840:intron 50.0%
!! TACAAGTGTCGGTGTCGTGA+CGG - chr2_4:33945973-33945992 Msa0325840:three_prime_UTR 50.0%
ATACGACACGTGTTCGACAC+CGG - chr2_4:33946025-33946044 Msa0325840:three_prime_UTR 50.0%
GCACGTACACTCCTTGGATT+AGG + chr2_4:33946057-33946076 None:intergenic 50.0%
TGATCTATGAAGCGCGGACA+CGG - chr2_4:33946416-33946435 Msa0325840:three_prime_UTR 50.0%
!! TGTCGGTGTCATGTCGATGT+CGG - chr2_4:33946505-33946524 Msa0325840:three_prime_UTR 50.0%
TATGAAGCACGGACACTCCT+CGG + chr2_4:33946566-33946585 None:intergenic 50.0%
GTGCCTTCCAATGCCAAGAT+GGG - chr2_4:33947002-33947021 Msa0325840:three_prime_UTR 50.0%
! CATCCCATCTTGGCATTGGA+AGG + chr2_4:33947008-33947027 None:intergenic 50.0%
!! TCGGCATTGGTGTAGTTCCA+AGG + chr2_4:33947269-33947288 None:intergenic 50.0%
AGTGAGCTAGCTCCTTTCAC+TGG - chr2_4:33947434-33947453 Msa0325840:intron 50.0%
CTGGCAGGCAATGAATTGAG+TGG - chr2_4:33947453-33947472 Msa0325840:intron 50.0%
GGCAGGCAATGAATTGAGTG+GGG - chr2_4:33947455-33947474 Msa0325840:intron 50.0%
GCAGGCAATGAATTGAGTGG+GGG - chr2_4:33947456-33947475 Msa0325840:intron 50.0%
GGCGCAATGACTAATGGCTT+AGG + chr2_4:33947573-33947592 None:intergenic 50.0%
AAGCCATTAGTCATTGCGCC+TGG - chr2_4:33947573-33947592 Msa0325840:intron 50.0%
!! CCAATTCGGCGAAAAGTACC+TGG + chr2_4:33947690-33947709 None:intergenic 50.0%
! CCAGGTACTTTTCGCCGAAT+TGG - chr2_4:33947687-33947706 Msa0325840:intron 50.0%
!! AGGAGTTGATACGCACCTTG+TGG - chr2_4:33947938-33947957 Msa0325840:intron 50.0%
AGGAACCTCAGCAACAGCAT+GGG - chr2_4:33948037-33948056 Msa0325840:intron 50.0%
TCAGGAGGGGCTTATAACAG+TGG - chr2_4:33948068-33948087 Msa0325840:intron 50.0%
! TGTCGGTGTTCGACACTTGT+TGG - chr2_4:33948217-33948236 Msa0325840:intron 50.0%
GTGTCGGACACGACATGTGT+CGG - chr2_4:33945995-33946014 Msa0325840:three_prime_UTR 55.0%
! GACACGACATGTGTCGGTGT+CGG - chr2_4:33946001-33946020 Msa0325840:three_prime_UTR 55.0%
! ACACGACATGTGTCGGTGTC+GGG - chr2_4:33946002-33946021 Msa0325840:three_prime_UTR 55.0%
TACGACACGTGTTCGACACC+GGG - chr2_4:33946026-33946045 Msa0325840:three_prime_UTR 55.0%
! TCCTTGGATTAGGCGTGTCC+CGG + chr2_4:33946047-33946066 None:intergenic 55.0%
ATGAAGCACGGACACTCCTC+GGG + chr2_4:33946565-33946584 None:intergenic 55.0%
!! GAGGAGTGTCCGTGCTTCAT+AGG - chr2_4:33946565-33946584 Msa0325840:three_prime_UTR 55.0%
GGTGCCTTCCAATGCCAAGA+TGG - chr2_4:33947001-33947020 Msa0325840:three_prime_UTR 55.0%
AGCTAGCTCCTTTCACTGGC+AGG - chr2_4:33947438-33947457 Msa0325840:intron 55.0%
GGCAATGAATTGAGTGGGGG+TGG - chr2_4:33947459-33947478 Msa0325840:intron 55.0%
GAATTGAGTGGGGGTGGAGT+TGG - chr2_4:33947465-33947484 Msa0325840:intron 55.0%
CCTCCAGGCGCAATGACTAA+TGG + chr2_4:33947579-33947598 None:intergenic 55.0%
CCATTAGTCATTGCGCCTGG+AGG - chr2_4:33947576-33947595 Msa0325840:intron 55.0%
!! GGCGAAAAGTACCTGGTCCA+AGG + chr2_4:33947683-33947702 None:intergenic 55.0%
CAGGAACCTCAGCAACAGCA+TGG - chr2_4:33948036-33948055 Msa0325840:intron 55.0%
ACCTCAGCAACAGCATGGGT+TGG - chr2_4:33948041-33948060 Msa0325840:intron 55.0%
ACCAACCCATGCTGTTGCTG+AGG + chr2_4:33948045-33948064 None:intergenic 55.0%
!! ACAGCATGGGTTGGTGAGTC+AGG - chr2_4:33948050-33948069 Msa0325840:intron 55.0%
!! CATGGGTTGGTGAGTCAGGA+GGG - chr2_4:33948054-33948073 Msa0325840:intron 55.0%
!! ATGGGTTGGTGAGTCAGGAG+GGG - chr2_4:33948055-33948074 Msa0325840:intron 55.0%
TGTAGCACAGACACCGACAC+TGG + chr2_4:33948311-33948330 None:intergenic 55.0%
!! TGTCGGTGTCGTGACGGTGT+CGG - chr2_4:33945979-33945998 Msa0325840:three_prime_UTR 60.0%
ACCGGGACACGCCTAATCCA+AGG - chr2_4:33946043-33946062 Msa0325840:three_prime_UTR 60.0%
ACACGACACGTGTCCGACAC+CGG - chr2_4:33946528-33946547 Msa0325840:three_prime_UTR 60.0%
!! GCATGGGTTGGTGAGTCAGG+AGG - chr2_4:33948053-33948072 Msa0325840:intron 60.0%
CACGACACGTGTCCGACACC+GGG - chr2_4:33946529-33946548 Msa0325840:three_prime_UTR 65.0%
GGTTAGGCGTGTCCCGGTGT+CGG + chr2_4:33946544-33946563 None:intergenic 65.0%
! TCCTCGGGTTAGGCGTGTCC+CGG + chr2_4:33946550-33946569 None:intergenic 65.0%
GCACGGACACTCCTCGGGTT+AGG + chr2_4:33946560-33946579 None:intergenic 65.0%
GTATGCACTCGCGTGAGCGC+AGG - chr2_4:33947082-33947101 Msa0325840:CDS 65.0%
ACCGGGACACGCCTAACCCG+AGG - chr2_4:33946546-33946565 Msa0325840:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 33945574 33948507 33945574 ID=Msa0325840;Name=Msa0325840
chr2_4 mRNA 33945574 33948507 33945574 ID=Msa0325840-mRNA-1;Parent=Msa0325840;Name=Msa0325840-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0.8|0.83|1|0.8|0.5|6|831|97
chr2_4 exon 33945574 33945698 33945574 ID=Msa0325840-mRNA-1:exon:10881;Parent=Msa0325840-mRNA-1
chr2_4 exon 33945952 33946141 33945952 ID=Msa0325840-mRNA-1:exon:10880;Parent=Msa0325840-mRNA-1
chr2_4 exon 33946391 33946621 33946391 ID=Msa0325840-mRNA-1:exon:10879;Parent=Msa0325840-mRNA-1
chr2_4 exon 33946737 33947209 33946737 ID=Msa0325840-mRNA-1:exon:10878;Parent=Msa0325840-mRNA-1
chr2_4 exon 33947331 33947367 33947331 ID=Msa0325840-mRNA-1:exon:10877;Parent=Msa0325840-mRNA-1
chr2_4 exon 33948439 33948507 33948439 ID=Msa0325840-mRNA-1:exon:10876;Parent=Msa0325840-mRNA-1
chr2_4 CDS 33948439 33948507 33948439 ID=Msa0325840-mRNA-1:cds;Parent=Msa0325840-mRNA-1
chr2_4 CDS 33947331 33947367 33947331 ID=Msa0325840-mRNA-1:cds;Parent=Msa0325840-mRNA-1
chr2_4 CDS 33947022 33947209 33947022 ID=Msa0325840-mRNA-1:cds;Parent=Msa0325840-mRNA-1
chr2_4 three_prime_UTR 33946737 33947021 33946737 ID=Msa0325840-mRNA-1:three_prime_utr;Parent=Msa0325840-mRNA-1
chr2_4 three_prime_UTR 33946391 33946621 33946391 ID=Msa0325840-mRNA-1:three_prime_utr;Parent=Msa0325840-mRNA-1
chr2_4 three_prime_UTR 33945952 33946141 33945952 ID=Msa0325840-mRNA-1:three_prime_utr;Parent=Msa0325840-mRNA-1
chr2_4 three_prime_UTR 33945574 33945698 33945574 ID=Msa0325840-mRNA-1:three_prime_utr;Parent=Msa0325840-mRNA-1
Gene Sequence

>Msa0325840

ATGGGTTCTTGGATTCAGATTATGTCTATATACTATTTGGCATGGTTCAGTTTTCTTGTTGTGAACACAGACCTCAACAACAAGATTTTCTTAAATGCAGTGAAAGCCTTTTCACCATTGAAACTGAGATTAGGTGGCAGTTTGCAAGATGAACTGGTATATGGGACTGATGATTACAACAAACCATGCACTCCTTTTGTTAGAAACACATCTGCAATGTTTGGATTCTCACAAGGGTGCCTTCCAATGCCAAGATGGGATGAACTTAATCATTTCTTCCAAAAATCAGGGTAA
Protein sequence

>Msa0325840

MGSWIQIMSIYYLAWFSFLVVNTDLNNKIFLNAVKAFSPLKLRLGGSLQDELVYGTDDYNKPCTPFVRNTSAMFGFSQGCLPMPRWDELNHFFQKSG*