AlfalfaGEDB | Msa1280560

M. sativa cultivar zhongmu-4 / Msa1280560

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1280560	A0A072TQ72	92.184	435	28	2	1	434	1	430	0.0	746

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002540	Msa1280560	0.819772	9.746033e-53	-8.615850e-47
Msa0008340	Msa1280560	0.820710	5.941134e-53	-8.615850e-47
Msa0008410	Msa1280560	0.822190	2.704813e-53	-8.615850e-47
Msa0012440	Msa1280560	0.810620	1.052832e-50	-8.615850e-47
Msa0027390	Msa1280560	0.801060	1.076060e-48	-8.615850e-47
Msa0032370	Msa1280560	0.842714	2.184029e-58	-8.615850e-47
Msa0044020	Msa1280560	0.801317	9.531270e-49	-8.615850e-47
Msa0047980	Msa1280560	0.839295	1.725456e-57	-8.615850e-47
Msa0057240	Msa1280560	0.815896	7.307486e-52	-8.615850e-47
Msa0064350	Msa1280560	0.809832	1.557008e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1280560	MtrunA17_Chr8g0350771	92.184	435	28	2	1	434	1	430	0.0	746

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1280560	AT4G00770.1	30.617	454	247	20	11	434	1	416	5.48e-35	135

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Find 86 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTCACGTACTTATAAG+TGG	0.528971	8_3:+19192045	Msa1280560:five_prime_UTR
AAAGGTGATAACGGCGGTGA+TGG	0.474408	8_3:-19192561	None:intergenic
AAAGTAATGAATGAAATGAT+AGG	0.394858	8_3:-19191900	None:intergenic
AAATCAAAGACATCATACGT+TGG	0.656713	8_3:+19192502	Msa1280560:CDS
AAATGGATAGGAGAGATGGG+TGG	0.646442	8_3:+19194378	Msa1280560:CDS
AACCAAAAGAGGAACGATGA+AGG	0.540411	8_3:+19194254	Msa1280560:CDS
AACGACGACGTTGAAGAGAG+TGG	0.596261	8_3:+19192711	Msa1280560:CDS
AACTTGCGTGATAAGGTTCG+TGG	0.577209	8_3:-19192405	None:intergenic
AAGAAATCCTTCAACATTCT+TGG	0.218670	8_3:-19192165	None:intergenic
AAGGAAATGGATAGGAGAGA+TGG	0.470909	8_3:+19194374	Msa1280560:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGAATGAAATGATAGGGT+TGG	-	chr8_3:19191898-19191917	None:intergenic	30.0%
AAACTTCACGTACTTATAAG+TGG	+	chr8_3:19192045-19192064	Msa1280560:five_prime_UTR	30.0%
TACTTATAAGTGGTGAATTG+CGG	+	chr8_3:19192055-19192074	Msa1280560:five_prime_UTR	30.0%
AAGAAATCCTTCAACATTCT+TGG	-	chr8_3:19192168-19192187	None:intergenic	30.0%
AATCGAAATCAAAACCAGTA+CGG	+	chr8_3:19192476-19192495	Msa1280560:CDS	30.0%
AAATCAAAGACATCATACGT+TGG	+	chr8_3:19192502-19192521	Msa1280560:CDS	30.0%
CGATAATCAAAAGGTGATAA+CGG	-	chr8_3:19192573-19192592	None:intergenic	30.0%
ACATTAGATACGATATGTGT+CGG	-	chr8_3:19193722-19193741	None:intergenic	30.0%
TAATGAAAATTGCTGAGGAT+TGG	+	chr8_3:19194291-19194310	Msa1280560:CDS	30.0%
AGATGCTTATATCAAGGAAA+TGG	+	chr8_3:19194361-19194380	Msa1280560:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	19191881	19194584	19191881	ID=Msa1280560;Name=Msa1280560
chr8_3	mRNA	19191881	19194584	19191881	ID=Msa1280560-mRNA-1;Parent=Msa1280560;Name=Msa1280560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=242\|1\|1\|1\|1\|1\|3\|94\|436
chr8_3	exon	19191881	19192797	19191881	ID=Msa1280560-mRNA-1:exon:7156;Parent=Msa1280560-mRNA-1
chr8_3	exon	19193443	19193545	19193443	ID=Msa1280560-mRNA-1:exon:7157;Parent=Msa1280560-mRNA-1
chr8_3	exon	19193958	19194584	19193958	ID=Msa1280560-mRNA-1:exon:7158;Parent=Msa1280560-mRNA-1
chr8_3	five_prime_UTR	19191881	19192122	19191881	ID=Msa1280560-mRNA-1:five_prime_utr;Parent=Msa1280560-mRNA-1
chr8_3	CDS	19192123	19192797	19192123	ID=Msa1280560-mRNA-1:cds;Parent=Msa1280560-mRNA-1
chr8_3	CDS	19193443	19193545	19193443	ID=Msa1280560-mRNA-1:cds;Parent=Msa1280560-mRNA-1
chr8_3	CDS	19193958	19194490	19193958	ID=Msa1280560-mRNA-1:cds;Parent=Msa1280560-mRNA-1
chr8_3	three_prime_UTR	19194491	19194584	19194491	ID=Msa1280560-mRNA-1:three_prime_utr;Parent=Msa1280560-mRNA-1

Gene Sequence

>Msa1280560

ATGGAGGTGCAACAAAGAAGATTCAACACCATGGAGAGAAGACCAAGAATGTTGAAGGATTTCTTAAGCGAGAATCCAAACTCAAACTCAAACTCATGTTCTTCAAGTGGATTCAAATCACTTCCAAGAACAATAAAACCAATTACCAACTCCATTCAAATCCATCATACACATTCATCATCATCATCATCATCATCAAAATTTCAAGCCCTAATAAAAACCATAAAAAACAACGTCACATTCTTCAAAATCACAAAACAACCCTCCACCATCCTCTCACTACCACGAACCTTATCACGCAAGTTATCATTAAAACGAAGAAGAACAAGCCAAAGATGCAAAGGTGAAAGAGAAATCGAAATCAAAACCAGTACGGTGAAAATCAAAGACATCATACGTTGGAAATCGTTTCGAGATATACAACAACCGTTGACGATTCCATCACCGCCGTTATCACCTTTTGATTATCGGCGTTTTGTGATGGAATCGTCGACGGTTACTACTGCAACGACTTGTTGTGGTAGTAGTAGTGACGGTGATTCTAGTTGGAGTGACGGTGATTTTTTTAGTGAGTTGTGGGATGCAGAAAACGACGACGTTGAAGAGAGTGGGAAAAAATTGTTTTCATCTTTTTTTGTCGGCAAGGATTCGGTAGCATCGTTCGTTGGGGACAAGGATGATTTGACTTGCCAAGATGAACAACACAGTCCAGTTTCAGTTCTTCGGGTTGCAGAAAATGAATTTTCAATCTTTGACCAAAGCCTAGCCAATATTGAAAGGAGAAAACAAAAATTCAGACAAACTTCTGATAAATCTGAGAGTCATGCAAAATTTGACCTGGTAACCTTAGATGAATGTCTCTCATTGGATGAAAATTTATACTATGGTGAAGAACATAAAGACGACGATAAGGAAGAGGAGGACCAAAAAACTGAAGAAGAAGTTTGGATTGAGGAAAGTGCAAAGCAATTACTACATATTGTTAAAGCAACAAGTTCATCAGTACAAAATTGTGATGACAATTTTGATACAGTTCTGCTGGAATTTTTCAAGGAAGAGCTAAGTGGTAATAGAAACCAAAAGAGGAACGATGAAGGATTTGAGTTGGAGATAATGAAAATTGCTGAGGATTGGATAAATGAGTCATTTGAAAAAACTTATGATATTGTGCATGTTAATAAAGATGCTTATATCAAGGAAATGGATAGGAGAGATGGGTGGAGTAGGTTTGATGAGGAGCTAGATGAGATGGTTATGGAGATTGAGGCAGCTATATTACATAGTTTGATTGATGATATTTTTCACCCCTAA

Protein sequence

>Msa1280560

MEVQQRRFNTMERRPRMLKDFLSENPNSNSNSCSSSGFKSLPRTIKPITNSIQIHHTHSSSSSSSSKFQALIKTIKNNVTFFKITKQPSTILSLPRTLSRKLSLKRRRTSQRCKGEREIEIKTSTVKIKDIIRWKSFRDIQQPLTIPSPPLSPFDYRRFVMESSTVTTATTCCGSSSDGDSSWSDGDFFSELWDAENDDVEESGKKLFSSFFVGKDSVASFVGDKDDLTCQDEQHSPVSVLRVAENEFSIFDQSLANIERRKQKFRQTSDKSESHAKFDLVTLDECLSLDENLYYGEEHKDDDKEEEDQKTEEEVWIEESAKQLLHIVKATSSSVQNCDDNFDTVLLEFFKEELSGNRNQKRNDEGFELEIMKIAEDWINESFEKTYDIVHVNKDAYIKEMDRRDGWSRFDEELDEMVMEIEAAILHSLIDDIFHP*