Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0337600 | XP_013465154.1 | 100.000 | 78 | 0 | 0 | 1 | 78 | 1 | 78 | 1.53e-45 | 150 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0337600 | sp|Q1G309|TAX2_ARATH | 73.239 | 71 | 19 | 0 | 8 | 78 | 3 | 73 | 1.58e-24 | 90.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0030800 | Msa0337600 | 0.822580 | 2.195313e-53 | -8.615850e-47 |
| Msa0078210 | Msa0337600 | 0.820099 | 8.204197e-53 | -8.615850e-47 |
| Msa0262360 | Msa0337600 | 0.825706 | 4.049822e-54 | -8.615850e-47 |
| Msa0300140 | Msa0337600 | 0.827057 | 1.930067e-54 | -8.615850e-47 |
| Msa0337600 | Msa1401110 | 0.811062 | 8.446856e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0337600 | MtrunA17_Chr2g0324911 | 100.000 | 78 | 0 | 0 | 1 | 78 | 1 | 78 | 1.41e-49 | 150 |
| Msa0337600 | MtrunA17_Chr2g0283451 | 60.811 | 74 | 29 | 0 | 5 | 78 | 7 | 80 | 6.04e-28 | 95.9 |
| Msa0337600 | MtrunA17_Chr4g0040991 | 58.974 | 78 | 31 | 1 | 1 | 78 | 1 | 77 | 5.56e-27 | 93.6 |
| Msa0337600 | MtrunA17_Chr2g0325441 | 53.846 | 78 | 35 | 1 | 1 | 78 | 1 | 77 | 1.35e-25 | 90.1 |
| Msa0337600 | MtrunA17_Chr4g0040491 | 72.727 | 77 | 21 | 0 | 2 | 78 | 3 | 79 | 4.26e-23 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0337600 | AT2G20562.1 | 73.239 | 71 | 19 | 0 | 8 | 78 | 3 | 73 | 1.60e-25 | 90.1 |
| Msa0337600 | AT2G31090.1 | 59.155 | 71 | 29 | 0 | 8 | 78 | 5 | 75 | 5.04e-21 | 79.0 |
Find 34 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCGCTGGATTGGCAAATT+TGG | 0.128393 | 2_4:-57641500 | Msa0337600:CDS |
| CTCTTGTTGGTGCAGTTATC+TGG | 0.152454 | 2_4:-57641883 | Msa0337600:CDS |
| TCTCCACTTCCCCTCGTTCT+TGG | 0.245405 | 2_4:-57642053 | Msa0337600:five_prime_UTR |
| CGAGGGGAAGTGGAGAAGTT+AGG | 0.288412 | 2_4:+57642060 | None:intergenic |
| AGAAAGAAAGAAAGAAAATA+TGG | 0.303892 | 2_4:-57641984 | Msa0337600:exon |
| GAGGGGAAGTGGAGAAGTTA+GGG | 0.336170 | 2_4:+57642061 | None:intergenic |
| TGTGGGTCTTGTAAAGCTTC+CGG | 0.396290 | 2_4:-57641476 | Msa0337600:CDS |
| CAAATTCAAAGGAAACAAAA+AGG | 0.397305 | 2_4:+57641358 | None:intergenic |
| TTTCTTTCTATATTCTTCAA+TGG | 0.404934 | 2_4:+57642014 | None:intergenic |
| TTATTTGTATCATGATTAAT+CGG | 0.408204 | 2_4:-57641334 | Msa0337600:three_prime_UTR |
| CCATGTTGCATTTGCTGCGC+TGG | 0.414638 | 2_4:-57641514 | Msa0337600:CDS |
| GTCAATATCGTAAACTATGA+AGG | 0.426268 | 2_4:+57641401 | None:intergenic |
| TTGCATTTGCTGCGCTGGAT+TGG | 0.438326 | 2_4:-57641509 | Msa0337600:CDS |
| ATCAAAGCCAAGAGAGCAAA+AGG | 0.457758 | 2_4:+57641911 | None:intergenic |
| TTCCGGTTCGTGTTCTTAAG+TGG | 0.464186 | 2_4:-57641459 | Msa0337600:CDS |
| TGGATTGGCAAATTTGGCTG+TGG | 0.481790 | 2_4:-57641494 | Msa0337600:CDS |
| GGTTGTGAATGCAGGCCATT+GGG | 0.491219 | 2_4:-57641950 | Msa0337600:CDS |
| TGACCAAGAACGAGGGGAAG+TGG | 0.494668 | 2_4:+57642050 | None:intergenic |
| GAAAATATGGCAGATGATGA+AGG | 0.507098 | 2_4:-57641971 | Msa0337600:exon |
| AACCACTTAAGAACACGAAC+CGG | 0.518107 | 2_4:+57641457 | None:intergenic |
| ATGATGAAGGTTGTGAATGC+AGG | 0.535019 | 2_4:-57641958 | Msa0337600:CDS |
| TAATCAAATATATAGTCAAA+CGG | 0.537893 | 2_4:+57641293 | None:intergenic |
| GGATTGGCAAATTTGGCTGT+GGG | 0.548115 | 2_4:-57641493 | Msa0337600:CDS |
| CCAGCGCAGCAAATGCAACA+TGG | 0.552638 | 2_4:+57641514 | None:intergenic |
| TCAATATCGTAAACTATGAA+GGG | 0.561342 | 2_4:+57641402 | None:intergenic |
| AGGTTGTGAATGCAGGCCAT+TGG | 0.583286 | 2_4:-57641951 | Msa0337600:CDS |
| CAGCGCAGCAAATGCAACAT+GGG | 0.600667 | 2_4:+57641515 | None:intergenic |
| ATACAAATAAGCAAATTCAA+AGG | 0.610702 | 2_4:+57641347 | None:intergenic |
| AGGGGAAGTGGAGAAGTTAG+GGG | 0.612620 | 2_4:+57642062 | None:intergenic |
| AGGGATCTTCATCTCTAACA+AGG | 0.631162 | 2_4:+57641421 | None:intergenic |
| GGGGAAGTGGAGAAGTTAGG+GGG | 0.647330 | 2_4:+57642063 | None:intergenic |
| TGTGAACGTGACCAAGAACG+AGG | 0.653521 | 2_4:+57642042 | None:intergenic |
| GTGAACGTGACCAAGAACGA+GGG | 0.665471 | 2_4:+57642043 | None:intergenic |
| TGAACGTGACCAAGAACGAG+GGG | 0.695224 | 2_4:+57642044 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTATTTTATTATTTTT+TGG | - | chr2_4:57641816-57641835 | Msa0337600:intron | 0.0% |
| !!! | TATTTTATTTTATTATTTTT+TGG | - | chr2_4:57641816-57641835 | Msa0337600:intron | 0.0% |
| !!! | TTTATTATTTTTTGGTTTTT+TGG | - | chr2_4:57641824-57641843 | Msa0337600:intron | 10.0% |
| !!! | TTTATTATTTTTTGGTTTTT+TGG | - | chr2_4:57641824-57641843 | Msa0337600:intron | 10.0% |
| !! | ATATGATTTCATAAAATAAC+AGG | + | chr2_4:57641636-57641655 | None:intergenic | 15.0% |
| !! | TTATTTGTATCATGATTAAT+CGG | - | chr2_4:57642081-57642100 | Msa0337600:five_prime_UTR | 15.0% |
| !! | TAATCAAATATATAGTCAAA+CGG | + | chr2_4:57642125-57642144 | None:intergenic | 15.0% |
| !! | ATATGATTTCATAAAATAAC+AGG | + | chr2_4:57641636-57641655 | None:intergenic | 15.0% |
| !! | TTATTTGTATCATGATTAAT+CGG | - | chr2_4:57642081-57642100 | Msa0337600:five_prime_UTR | 15.0% |
| !! | TAATCAAATATATAGTCAAA+CGG | + | chr2_4:57642125-57642144 | None:intergenic | 15.0% |
| !! | TTTCTTTCTATATTCTTCAA+TGG | + | chr2_4:57641404-57641423 | None:intergenic | 20.0% |
| !! | AGAAAGAAAGAAAGAAAATA+TGG | - | chr2_4:57641431-57641450 | Msa0337600:exon | 20.0% |
| !! | AAAGATAAAACTTGATATCT+AGG | - | chr2_4:57641703-57641722 | Msa0337600:intron | 20.0% |
| !! | AAGATAAAACTTGATATCTA+GGG | - | chr2_4:57641704-57641723 | Msa0337600:intron | 20.0% |
| !! | ATACAAATAAGCAAATTCAA+AGG | + | chr2_4:57642071-57642090 | None:intergenic | 20.0% |
| !! | TTTCTTTCTATATTCTTCAA+TGG | + | chr2_4:57641404-57641423 | None:intergenic | 20.0% |
| !! | AGAAAGAAAGAAAGAAAATA+TGG | - | chr2_4:57641431-57641450 | Msa0337600:exon | 20.0% |
| !! | AAAGATAAAACTTGATATCT+AGG | - | chr2_4:57641703-57641722 | Msa0337600:intron | 20.0% |
| !! | AAGATAAAACTTGATATCTA+GGG | - | chr2_4:57641704-57641723 | Msa0337600:intron | 20.0% |
| !! | ATACAAATAAGCAAATTCAA+AGG | + | chr2_4:57642071-57642090 | None:intergenic | 20.0% |
| ! | TCAATATCGTAAACTATGAA+GGG | + | chr2_4:57642016-57642035 | None:intergenic | 25.0% |
| ! | CAAATTCAAAGGAAACAAAA+AGG | + | chr2_4:57642060-57642079 | None:intergenic | 25.0% |
| ! | TCAATATCGTAAACTATGAA+GGG | + | chr2_4:57642016-57642035 | None:intergenic | 25.0% |
| ! | CAAATTCAAAGGAAACAAAA+AGG | + | chr2_4:57642060-57642079 | None:intergenic | 25.0% |
| !! | GCTTTGATTTTATCTCTTGT+TGG | - | chr2_4:57641519-57641538 | Msa0337600:CDS | 30.0% |
| GTCAATATCGTAAACTATGA+AGG | + | chr2_4:57642017-57642036 | None:intergenic | 30.0% | |
| !! | GCTTTGATTTTATCTCTTGT+TGG | - | chr2_4:57641519-57641538 | Msa0337600:CDS | 30.0% |
| GTCAATATCGTAAACTATGA+AGG | + | chr2_4:57642017-57642036 | None:intergenic | 30.0% | |
| GAAAATATGGCAGATGATGA+AGG | - | chr2_4:57641444-57641463 | Msa0337600:CDS | 35.0% | |
| AATCCAAGCAAAAAACCCAA+TGG | + | chr2_4:57641483-57641502 | None:intergenic | 35.0% | |
| ! | GTGCAGTTATCTGGATTTTT+GGG | - | chr2_4:57641541-57641560 | Msa0337600:intron | 35.0% |
| GAAAATATGGCAGATGATGA+AGG | - | chr2_4:57641444-57641463 | Msa0337600:CDS | 35.0% | |
| AATCCAAGCAAAAAACCCAA+TGG | + | chr2_4:57641483-57641502 | None:intergenic | 35.0% | |
| ! | GTGCAGTTATCTGGATTTTT+GGG | - | chr2_4:57641541-57641560 | Msa0337600:intron | 35.0% |
| ATGATGAAGGTTGTGAATGC+AGG | - | chr2_4:57641457-57641476 | Msa0337600:CDS | 40.0% | |
| ! | TGGATTACCTTTTGCTCTCT+TGG | - | chr2_4:57641497-57641516 | Msa0337600:CDS | 40.0% |
| ATCAAAGCCAAGAGAGCAAA+AGG | + | chr2_4:57641507-57641526 | None:intergenic | 40.0% | |
| ! | GGTGCAGTTATCTGGATTTT+TGG | - | chr2_4:57641540-57641559 | Msa0337600:intron | 40.0% |
| AACCACTTAAGAACACGAAC+CGG | + | chr2_4:57641961-57641980 | None:intergenic | 40.0% | |
| AGGGATCTTCATCTCTAACA+AGG | + | chr2_4:57641997-57642016 | None:intergenic | 40.0% | |
| ATGATGAAGGTTGTGAATGC+AGG | - | chr2_4:57641457-57641476 | Msa0337600:CDS | 40.0% | |
| ! | TGGATTACCTTTTGCTCTCT+TGG | - | chr2_4:57641497-57641516 | Msa0337600:CDS | 40.0% |
| ATCAAAGCCAAGAGAGCAAA+AGG | + | chr2_4:57641507-57641526 | None:intergenic | 40.0% | |
| ! | GGTGCAGTTATCTGGATTTT+TGG | - | chr2_4:57641540-57641559 | Msa0337600:intron | 40.0% |
| AACCACTTAAGAACACGAAC+CGG | + | chr2_4:57641961-57641980 | None:intergenic | 40.0% | |
| AGGGATCTTCATCTCTAACA+AGG | + | chr2_4:57641997-57642016 | None:intergenic | 40.0% | |
| !!! | AGGCCATTGGGTTTTTTGCT+TGG | - | chr2_4:57641477-57641496 | Msa0337600:CDS | 45.0% |
| CTCTTGTTGGTGCAGTTATC+TGG | - | chr2_4:57641532-57641551 | Msa0337600:CDS | 45.0% | |
| TGCGCATCAACTACTTTCAC+CGG | - | chr2_4:57641745-57641764 | Msa0337600:intron | 45.0% | |
| ! | TTATTAAGCACCGTTGCGTC+CGG | + | chr2_4:57641767-57641786 | None:intergenic | 45.0% |
| !! | TGGATTGGCAAATTTGGCTG+TGG | - | chr2_4:57641921-57641940 | Msa0337600:CDS | 45.0% |
| ! | GGATTGGCAAATTTGGCTGT+GGG | - | chr2_4:57641922-57641941 | Msa0337600:CDS | 45.0% |
| ! | TGTGGGTCTTGTAAAGCTTC+CGG | - | chr2_4:57641939-57641958 | Msa0337600:CDS | 45.0% |
| ! | TTCCGGTTCGTGTTCTTAAG+TGG | - | chr2_4:57641956-57641975 | Msa0337600:CDS | 45.0% |
| !!! | AGGCCATTGGGTTTTTTGCT+TGG | - | chr2_4:57641477-57641496 | Msa0337600:CDS | 45.0% |
| CTCTTGTTGGTGCAGTTATC+TGG | - | chr2_4:57641532-57641551 | Msa0337600:CDS | 45.0% | |
| TGCGCATCAACTACTTTCAC+CGG | - | chr2_4:57641745-57641764 | Msa0337600:intron | 45.0% | |
| ! | TTATTAAGCACCGTTGCGTC+CGG | + | chr2_4:57641767-57641786 | None:intergenic | 45.0% |
| !! | TGGATTGGCAAATTTGGCTG+TGG | - | chr2_4:57641921-57641940 | Msa0337600:CDS | 45.0% |
| ! | GGATTGGCAAATTTGGCTGT+GGG | - | chr2_4:57641922-57641941 | Msa0337600:CDS | 45.0% |
| ! | TGTGGGTCTTGTAAAGCTTC+CGG | - | chr2_4:57641939-57641958 | Msa0337600:CDS | 45.0% |
| ! | TTCCGGTTCGTGTTCTTAAG+TGG | - | chr2_4:57641956-57641975 | Msa0337600:CDS | 45.0% |
| AGGGGAAGTGGAGAAGTTAG+GGG | + | chr2_4:57641356-57641375 | None:intergenic | 50.0% | |
| GAGGGGAAGTGGAGAAGTTA+GGG | + | chr2_4:57641357-57641376 | None:intergenic | 50.0% | |
| TGAACGTGACCAAGAACGAG+GGG | + | chr2_4:57641374-57641393 | None:intergenic | 50.0% | |
| GTGAACGTGACCAAGAACGA+GGG | + | chr2_4:57641375-57641394 | None:intergenic | 50.0% | |
| TGTGAACGTGACCAAGAACG+AGG | + | chr2_4:57641376-57641395 | None:intergenic | 50.0% | |
| AGGTTGTGAATGCAGGCCAT+TGG | - | chr2_4:57641464-57641483 | Msa0337600:CDS | 50.0% | |
| GGTTGTGAATGCAGGCCATT+GGG | - | chr2_4:57641465-57641484 | Msa0337600:CDS | 50.0% | |
| ACTACTTTCACCGGACGCAA+CGG | - | chr2_4:57641754-57641773 | Msa0337600:intron | 50.0% | |
| CAGCGCAGCAAATGCAACAT+GGG | + | chr2_4:57641903-57641922 | None:intergenic | 50.0% | |
| ! | TTGCATTTGCTGCGCTGGAT+TGG | - | chr2_4:57641906-57641925 | Msa0337600:CDS | 50.0% |
| !! | CTGCGCTGGATTGGCAAATT+TGG | - | chr2_4:57641915-57641934 | Msa0337600:CDS | 50.0% |
| AGGGGAAGTGGAGAAGTTAG+GGG | + | chr2_4:57641356-57641375 | None:intergenic | 50.0% | |
| GAGGGGAAGTGGAGAAGTTA+GGG | + | chr2_4:57641357-57641376 | None:intergenic | 50.0% | |
| TGAACGTGACCAAGAACGAG+GGG | + | chr2_4:57641374-57641393 | None:intergenic | 50.0% | |
| GTGAACGTGACCAAGAACGA+GGG | + | chr2_4:57641375-57641394 | None:intergenic | 50.0% | |
| TGTGAACGTGACCAAGAACG+AGG | + | chr2_4:57641376-57641395 | None:intergenic | 50.0% | |
| AGGTTGTGAATGCAGGCCAT+TGG | - | chr2_4:57641464-57641483 | Msa0337600:CDS | 50.0% | |
| GGTTGTGAATGCAGGCCATT+GGG | - | chr2_4:57641465-57641484 | Msa0337600:CDS | 50.0% | |
| ACTACTTTCACCGGACGCAA+CGG | - | chr2_4:57641754-57641773 | Msa0337600:intron | 50.0% | |
| CAGCGCAGCAAATGCAACAT+GGG | + | chr2_4:57641903-57641922 | None:intergenic | 50.0% | |
| ! | TTGCATTTGCTGCGCTGGAT+TGG | - | chr2_4:57641906-57641925 | Msa0337600:CDS | 50.0% |
| !! | CTGCGCTGGATTGGCAAATT+TGG | - | chr2_4:57641915-57641934 | Msa0337600:CDS | 50.0% |
| GGGGAAGTGGAGAAGTTAGG+GGG | + | chr2_4:57641355-57641374 | None:intergenic | 55.0% | |
| CGAGGGGAAGTGGAGAAGTT+AGG | + | chr2_4:57641358-57641377 | None:intergenic | 55.0% | |
| TCTCCACTTCCCCTCGTTCT+TGG | - | chr2_4:57641362-57641381 | Msa0337600:three_prime_UTR | 55.0% | |
| TGACCAAGAACGAGGGGAAG+TGG | + | chr2_4:57641368-57641387 | None:intergenic | 55.0% | |
| CCAGCGCAGCAAATGCAACA+TGG | + | chr2_4:57641904-57641923 | None:intergenic | 55.0% | |
| ! | CCATGTTGCATTTGCTGCGC+TGG | - | chr2_4:57641901-57641920 | Msa0337600:CDS | 55.0% |
| GGGGAAGTGGAGAAGTTAGG+GGG | + | chr2_4:57641355-57641374 | None:intergenic | 55.0% | |
| CGAGGGGAAGTGGAGAAGTT+AGG | + | chr2_4:57641358-57641377 | None:intergenic | 55.0% | |
| TCTCCACTTCCCCTCGTTCT+TGG | - | chr2_4:57641362-57641381 | Msa0337600:three_prime_UTR | 55.0% | |
| TGACCAAGAACGAGGGGAAG+TGG | + | chr2_4:57641368-57641387 | None:intergenic | 55.0% | |
| CCAGCGCAGCAAATGCAACA+TGG | + | chr2_4:57641904-57641923 | None:intergenic | 55.0% | |
| ! | CCATGTTGCATTTGCTGCGC+TGG | - | chr2_4:57641901-57641920 | Msa0337600:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 57641290 | 57642147 | 57641290 | ID=Msa0337600;Name=Msa0337600 |
| chr2_4 | mRNA | 57641290 | 57642147 | 57641290 | ID=Msa0337600-mRNA-1;Parent=Msa0337600;Name=Msa0337600-mRNA-1;_AED=0.01;_eAED=0.01;_QI=160|1|1|1|1|1|2|145|78 |
| chr2_4 | exon | 57641290 | 57641558 | 57641290 | ID=Msa0337600-mRNA-1:exon:17577;Parent=Msa0337600-mRNA-1 |
| chr2_4 | exon | 57641875 | 57642147 | 57641875 | ID=Msa0337600-mRNA-1:exon:17576;Parent=Msa0337600-mRNA-1 |
| chr2_4 | five_prime_UTR | 57641988 | 57642147 | 57641988 | ID=Msa0337600-mRNA-1:five_prime_utr;Parent=Msa0337600-mRNA-1 |
| chr2_4 | CDS | 57641875 | 57641987 | 57641875 | ID=Msa0337600-mRNA-1:cds;Parent=Msa0337600-mRNA-1 |
| chr2_4 | CDS | 57641435 | 57641558 | 57641435 | ID=Msa0337600-mRNA-1:cds;Parent=Msa0337600-mRNA-1 |
| chr2_4 | three_prime_UTR | 57641290 | 57641434 | 57641290 | ID=Msa0337600-mRNA-1:three_prime_utr;Parent=Msa0337600-mRNA-1 |
| Gene Sequence |
| Protein sequence |
