AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0300140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300140 MtrunA17_Chr2g0324911 100.000 78 0 0 1 78 1 78 1.41e-49 150
Msa0300140 MtrunA17_Chr2g0283451 60.811 74 29 0 5 78 7 80 6.04e-28 95.9
Msa0300140 MtrunA17_Chr4g0040991 58.974 78 31 1 1 78 1 77 5.56e-27 93.6
Msa0300140 MtrunA17_Chr2g0325441 53.846 78 35 1 1 78 1 77 1.35e-25 90.1
Msa0300140 MtrunA17_Chr4g0040491 72.727 77 21 0 2 78 3 79 4.26e-23 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300140 AT2G20562.1 73.239 71 19 0 8 78 3 73 1.60e-25 90.1
Msa0300140 AT2G31090.1 59.155 71 29 0 8 78 5 75 5.04e-21 79.0

Find 33 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCGCTGGATTGGCAAATT+TGG 0.128393 2_3:+55506868 Msa0300140:CDS
CTCTTGTTGGTGCAGTTATC+TGG 0.152454 2_3:+55506485 Msa0300140:CDS
TCTCCACTTCCCCTCGTTCT+TGG 0.245405 2_3:+55506319 Msa0300140:five_prime_UTR
CGAGGGGAAGTGGAGAAGTT+AGG 0.288412 2_3:-55506312 None:intergenic
AGAAAGAAAGAAAGAAAATA+TGG 0.303892 2_3:+55506384 Msa0300140:exon
GAGGGGAAGTGGAGAAGTTA+GGG 0.336170 2_3:-55506311 None:intergenic
TTTCTTTCTATATTCTTCAA+TGG 0.404934 2_3:-55506358 None:intergenic
CAAATTCAAAGAAAACAAAA+AGG 0.405497 2_3:-55507010 None:intergenic
CCATGTTGCATTTGCTGCGC+TGG 0.414638 2_3:+55506854 Msa0300140:CDS
TTGCATTTGCTGCGCTGGAT+TGG 0.438326 2_3:+55506859 Msa0300140:CDS
ATCAAAGCCAAGAGAGCAAA+AGG 0.457758 2_3:-55506457 None:intergenic
GTAAATATCGTAAACTATGA+AGG 0.463185 2_3:-55506967 None:intergenic
TTCCAGTTCGTGTTCTTAAG+TGG 0.471610 2_3:+55506909 Msa0300140:CDS
AACCACTTAAGAACACGAAC+TGG 0.473947 2_3:-55506911 None:intergenic
TGGATTGGCAAATTTGGCTG+TGG 0.481790 2_3:+55506874 Msa0300140:CDS
GGTTGTGAATGCAGGCCATT+GGG 0.491219 2_3:+55506418 Msa0300140:CDS
TGACCAAGAACGAGGGGAAG+TGG 0.494668 2_3:-55506322 None:intergenic
GAAAATATGGCAGATGATGA+AGG 0.507098 2_3:+55506397 Msa0300140:exon
TAAATATCGTAAACTATGAA+GGG 0.517114 2_3:-55506966 None:intergenic
ATGATGAAGGTTGTGAATGC+AGG 0.535019 2_3:+55506410 Msa0300140:CDS
AATCAAAATATATAGACAAA+CGG 0.544954 2_3:-55507075 None:intergenic
GGATTGGCAAATTTGGCTGT+GGG 0.548115 2_3:+55506875 Msa0300140:CDS
CCAGCGCAGCAAATGCAACA+TGG 0.552638 2_3:-55506854 None:intergenic
TTATTTGTATCATGATTCAT+CGG 0.553612 2_3:+55507034 Msa0300140:three_prime_UTR
AGGTTGTGAATGCAGGCCAT+TGG 0.583286 2_3:+55506417 Msa0300140:CDS
CAGCGCAGCAAATGCAACAT+GGG 0.600667 2_3:-55506853 None:intergenic
AGGGGAAGTGGAGAAGTTAG+GGG 0.612620 2_3:-55506310 None:intergenic
AGGGGTCTTCATCTCTAACA+AGG 0.633868 2_3:-55506947 None:intergenic
AAATATCGTAAACTATGAAG+GGG 0.645560 2_3:-55506965 None:intergenic
GGGGAAGTGGAGAAGTTAGG+GGG 0.647330 2_3:-55506309 None:intergenic
TGTGAACGTGACCAAGAACG+AGG 0.653521 2_3:-55506330 None:intergenic
GTGAACGTGACCAAGAACGA+GGG 0.665471 2_3:-55506329 None:intergenic
TGAACGTGACCAAGAACGAG+GGG 0.695224 2_3:-55506328 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTATTTTATTATTTTT+TGG + chr2_3:55506766-55506785 Msa0300140:intron 0.0%
!!! TATTTTATTTTATTATTTTT+TGG + chr2_3:55506766-55506785 Msa0300140:intron 0.0%
!!! TTTATTATTTTTTGGTTTTT+TGG + chr2_3:55506774-55506793 Msa0300140:intron 10.0%
!!! TTTATTATTTTTTGGTTTTT+TGG + chr2_3:55506774-55506793 Msa0300140:intron 10.0%
!! ATATGATTTCATAAAATAAC+AGG - chr2_3:55506589-55506608 None:intergenic 15.0%
!! AATCAAAATATATAGACAAA+CGG - chr2_3:55507078-55507097 None:intergenic 15.0%
!! ATATGATTTCATAAAATAAC+AGG - chr2_3:55506589-55506608 None:intergenic 15.0%
!! AATCAAAATATATAGACAAA+CGG - chr2_3:55507078-55507097 None:intergenic 15.0%
!! TTTCTTTCTATATTCTTCAA+TGG - chr2_3:55506361-55506380 None:intergenic 20.0%
!! AGAAAGAAAGAAAGAAAATA+TGG + chr2_3:55506384-55506403 Msa0300140:exon 20.0%
!! AAAGATAAAACTTGATATCT+AGG + chr2_3:55506656-55506675 Msa0300140:intron 20.0%
!! AAGATAAAACTTGATATCTA+GGG + chr2_3:55506657-55506676 Msa0300140:intron 20.0%
!! TAAATATCGTAAACTATGAA+GGG - chr2_3:55506969-55506988 None:intergenic 20.0%
!! CAAATTCAAAGAAAACAAAA+AGG - chr2_3:55507013-55507032 None:intergenic 20.0%
!! TTATTTGTATCATGATTCAT+CGG + chr2_3:55507034-55507053 Msa0300140:three_prime_UTR 20.0%
!! TTTCTTTCTATATTCTTCAA+TGG - chr2_3:55506361-55506380 None:intergenic 20.0%
!! AGAAAGAAAGAAAGAAAATA+TGG + chr2_3:55506384-55506403 Msa0300140:exon 20.0%
!! AAAGATAAAACTTGATATCT+AGG + chr2_3:55506656-55506675 Msa0300140:intron 20.0%
!! AAGATAAAACTTGATATCTA+GGG + chr2_3:55506657-55506676 Msa0300140:intron 20.0%
!! TAAATATCGTAAACTATGAA+GGG - chr2_3:55506969-55506988 None:intergenic 20.0%
!! CAAATTCAAAGAAAACAAAA+AGG - chr2_3:55507013-55507032 None:intergenic 20.0%
!! TTATTTGTATCATGATTCAT+CGG + chr2_3:55507034-55507053 Msa0300140:three_prime_UTR 20.0%
! AAATATCGTAAACTATGAAG+GGG - chr2_3:55506968-55506987 None:intergenic 25.0%
! GTAAATATCGTAAACTATGA+AGG - chr2_3:55506970-55506989 None:intergenic 25.0%
! AAATATCGTAAACTATGAAG+GGG - chr2_3:55506968-55506987 None:intergenic 25.0%
! GTAAATATCGTAAACTATGA+AGG - chr2_3:55506970-55506989 None:intergenic 25.0%
!! GCTTTGATTTTATCTCTTGT+TGG + chr2_3:55506472-55506491 Msa0300140:CDS 30.0%
!! GCTTTGATTTTATCTCTTGT+TGG + chr2_3:55506472-55506491 Msa0300140:CDS 30.0%
GAAAATATGGCAGATGATGA+AGG + chr2_3:55506397-55506416 Msa0300140:exon 35.0%
AATCCAAGCAAAAAACCCAA+TGG - chr2_3:55506436-55506455 None:intergenic 35.0%
! GTGCAGTTATCTGGATTTTT+GGG + chr2_3:55506494-55506513 Msa0300140:CDS 35.0%
GAAAATATGGCAGATGATGA+AGG + chr2_3:55506397-55506416 Msa0300140:exon 35.0%
AATCCAAGCAAAAAACCCAA+TGG - chr2_3:55506436-55506455 None:intergenic 35.0%
! GTGCAGTTATCTGGATTTTT+GGG + chr2_3:55506494-55506513 Msa0300140:CDS 35.0%
ATGATGAAGGTTGTGAATGC+AGG + chr2_3:55506410-55506429 Msa0300140:CDS 40.0%
! TGGATTACCTTTTGCTCTCT+TGG + chr2_3:55506450-55506469 Msa0300140:CDS 40.0%
ATCAAAGCCAAGAGAGCAAA+AGG - chr2_3:55506460-55506479 None:intergenic 40.0%
! GGTGCAGTTATCTGGATTTT+TGG + chr2_3:55506493-55506512 Msa0300140:CDS 40.0%
TTCCAGTTCGTGTTCTTAAG+TGG + chr2_3:55506909-55506928 Msa0300140:CDS 40.0%
AACCACTTAAGAACACGAAC+TGG - chr2_3:55506914-55506933 None:intergenic 40.0%
ATGATGAAGGTTGTGAATGC+AGG + chr2_3:55506410-55506429 Msa0300140:CDS 40.0%
! TGGATTACCTTTTGCTCTCT+TGG + chr2_3:55506450-55506469 Msa0300140:CDS 40.0%
ATCAAAGCCAAGAGAGCAAA+AGG - chr2_3:55506460-55506479 None:intergenic 40.0%
! GGTGCAGTTATCTGGATTTT+TGG + chr2_3:55506493-55506512 Msa0300140:CDS 40.0%
TTCCAGTTCGTGTTCTTAAG+TGG + chr2_3:55506909-55506928 Msa0300140:CDS 40.0%
AACCACTTAAGAACACGAAC+TGG - chr2_3:55506914-55506933 None:intergenic 40.0%
!!! AGGCCATTGGGTTTTTTGCT+TGG + chr2_3:55506430-55506449 Msa0300140:CDS 45.0%
CTCTTGTTGGTGCAGTTATC+TGG + chr2_3:55506485-55506504 Msa0300140:CDS 45.0%
TGCGCATCAACTACTTTCAC+CGG + chr2_3:55506700-55506719 Msa0300140:intron 45.0%
!! TGGATTGGCAAATTTGGCTG+TGG + chr2_3:55506874-55506893 Msa0300140:CDS 45.0%
! GGATTGGCAAATTTGGCTGT+GGG + chr2_3:55506875-55506894 Msa0300140:CDS 45.0%
AGGGGTCTTCATCTCTAACA+AGG - chr2_3:55506950-55506969 None:intergenic 45.0%
!!! AGGCCATTGGGTTTTTTGCT+TGG + chr2_3:55506430-55506449 Msa0300140:CDS 45.0%
CTCTTGTTGGTGCAGTTATC+TGG + chr2_3:55506485-55506504 Msa0300140:CDS 45.0%
TGCGCATCAACTACTTTCAC+CGG + chr2_3:55506700-55506719 Msa0300140:intron 45.0%
!! TGGATTGGCAAATTTGGCTG+TGG + chr2_3:55506874-55506893 Msa0300140:CDS 45.0%
! GGATTGGCAAATTTGGCTGT+GGG + chr2_3:55506875-55506894 Msa0300140:CDS 45.0%
AGGGGTCTTCATCTCTAACA+AGG - chr2_3:55506950-55506969 None:intergenic 45.0%
AGGGGAAGTGGAGAAGTTAG+GGG - chr2_3:55506313-55506332 None:intergenic 50.0%
GAGGGGAAGTGGAGAAGTTA+GGG - chr2_3:55506314-55506333 None:intergenic 50.0%
TGAACGTGACCAAGAACGAG+GGG - chr2_3:55506331-55506350 None:intergenic 50.0%
GTGAACGTGACCAAGAACGA+GGG - chr2_3:55506332-55506351 None:intergenic 50.0%
TGTGAACGTGACCAAGAACG+AGG - chr2_3:55506333-55506352 None:intergenic 50.0%
AGGTTGTGAATGCAGGCCAT+TGG + chr2_3:55506417-55506436 Msa0300140:CDS 50.0%
GGTTGTGAATGCAGGCCATT+GGG + chr2_3:55506418-55506437 Msa0300140:CDS 50.0%
CAGCGCAGCAAATGCAACAT+GGG - chr2_3:55506856-55506875 None:intergenic 50.0%
! TTGCATTTGCTGCGCTGGAT+TGG + chr2_3:55506859-55506878 Msa0300140:CDS 50.0%
!! CTGCGCTGGATTGGCAAATT+TGG + chr2_3:55506868-55506887 Msa0300140:CDS 50.0%
AGGGGAAGTGGAGAAGTTAG+GGG - chr2_3:55506313-55506332 None:intergenic 50.0%
GAGGGGAAGTGGAGAAGTTA+GGG - chr2_3:55506314-55506333 None:intergenic 50.0%
TGAACGTGACCAAGAACGAG+GGG - chr2_3:55506331-55506350 None:intergenic 50.0%
GTGAACGTGACCAAGAACGA+GGG - chr2_3:55506332-55506351 None:intergenic 50.0%
TGTGAACGTGACCAAGAACG+AGG - chr2_3:55506333-55506352 None:intergenic 50.0%
AGGTTGTGAATGCAGGCCAT+TGG + chr2_3:55506417-55506436 Msa0300140:CDS 50.0%
GGTTGTGAATGCAGGCCATT+GGG + chr2_3:55506418-55506437 Msa0300140:CDS 50.0%
CAGCGCAGCAAATGCAACAT+GGG - chr2_3:55506856-55506875 None:intergenic 50.0%
! TTGCATTTGCTGCGCTGGAT+TGG + chr2_3:55506859-55506878 Msa0300140:CDS 50.0%
!! CTGCGCTGGATTGGCAAATT+TGG + chr2_3:55506868-55506887 Msa0300140:CDS 50.0%
GGGGAAGTGGAGAAGTTAGG+GGG - chr2_3:55506312-55506331 None:intergenic 55.0%
CGAGGGGAAGTGGAGAAGTT+AGG - chr2_3:55506315-55506334 None:intergenic 55.0%
TCTCCACTTCCCCTCGTTCT+TGG + chr2_3:55506319-55506338 Msa0300140:five_prime_UTR 55.0%
TGACCAAGAACGAGGGGAAG+TGG - chr2_3:55506325-55506344 None:intergenic 55.0%
ACTACTTTCACCGGACGCAG+CGG + chr2_3:55506709-55506728 Msa0300140:intron 55.0%
CCAGCGCAGCAAATGCAACA+TGG - chr2_3:55506857-55506876 None:intergenic 55.0%
! CCATGTTGCATTTGCTGCGC+TGG + chr2_3:55506854-55506873 Msa0300140:CDS 55.0%
GGGGAAGTGGAGAAGTTAGG+GGG - chr2_3:55506312-55506331 None:intergenic 55.0%
CGAGGGGAAGTGGAGAAGTT+AGG - chr2_3:55506315-55506334 None:intergenic 55.0%
TCTCCACTTCCCCTCGTTCT+TGG + chr2_3:55506319-55506338 Msa0300140:five_prime_UTR 55.0%
TGACCAAGAACGAGGGGAAG+TGG - chr2_3:55506325-55506344 None:intergenic 55.0%
ACTACTTTCACCGGACGCAG+CGG + chr2_3:55506709-55506728 Msa0300140:intron 55.0%
CCAGCGCAGCAAATGCAACA+TGG - chr2_3:55506857-55506876 None:intergenic 55.0%
! CCATGTTGCATTTGCTGCGC+TGG + chr2_3:55506854-55506873 Msa0300140:CDS 55.0%
! TGACTAAGCACCGCTGCGTC+CGG - chr2_3:55506722-55506741 None:intergenic 60.0%
! TGACTAAGCACCGCTGCGTC+CGG - chr2_3:55506722-55506741 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 55506247 55507101 55506247 ID=Msa0300140;Name=Msa0300140
chr2_3 mRNA 55506247 55507101 55506247 ID=Msa0300140-mRNA-1;Parent=Msa0300140;Name=Msa0300140-mRNA-1;_AED=0.01;_eAED=0.01;_QI=156|1|1|1|1|1|2|146|78
chr2_3 exon 55506247 55506515 55506247 ID=Msa0300140-mRNA-1:exon:17754;Parent=Msa0300140-mRNA-1
chr2_3 exon 55506832 55507101 55506832 ID=Msa0300140-mRNA-1:exon:17755;Parent=Msa0300140-mRNA-1
chr2_3 five_prime_UTR 55506247 55506402 55506247 ID=Msa0300140-mRNA-1:five_prime_utr;Parent=Msa0300140-mRNA-1
chr2_3 CDS 55506403 55506515 55506403 ID=Msa0300140-mRNA-1:cds;Parent=Msa0300140-mRNA-1
chr2_3 CDS 55506832 55506955 55506832 ID=Msa0300140-mRNA-1:cds;Parent=Msa0300140-mRNA-1
chr2_3 three_prime_UTR 55506956 55507101 55506956 ID=Msa0300140-mRNA-1:three_prime_utr;Parent=Msa0300140-mRNA-1
Gene Sequence

>Msa0300140

ATGGCAGATGATGAAGGTTGTGAATGCAGGCCATTGGGTTTTTTGCTTGGATTACCTTTTGCTCTCTTGGCTTTGATTTTATCTCTTGTTGGTGCAGTTATCTGGATTTTTGGATCTATATTGAGTTGTTTGTGCCCATGTTGCATTTGCTGCGCTGGATTGGCAAATTTGGCTGTGGGTCTTGTAAAGCTTCCAGTTCGTGTTCTTAAGTGGTTTACTCGACAAATTCCTTGTTAG

Protein sequence

>Msa0300140

MADDEGCECRPLGFLLGLPFALLALILSLVGAVIWIFGSILSCLCPCCICCAGLANLAVGLVKLPVRVLKWFTRQIPC*