AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1401110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1401110 MtrunA17_Chr7g0274321 79.381 97 7 3 22 109 21 113 2.61e-46 144
Msa1401110 MtrunA17_Chr1g0190491 45.238 84 27 3 1 68 1 81 5.99e-15 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCTGAAATTCATGTTGC+TGG 0.338103 tig0022499:-98861 Msa1401110:CDS
AATCCATGCTCTACAGTTCC+TGG 0.376944 tig0022499:-98906 Msa1401110:CDS
CAGTTCCTGGTCGCAGTAAA+GGG 0.426702 tig0022499:-98893 Msa1401110:CDS
ACAGTTCCTGGTCGCAGTAA+AGG 0.429935 tig0022499:-98894 Msa1401110:CDS
TTCTTCGTCGAACCTTTCAC+TGG 0.435414 tig0022499:+98927 None:intergenic
CAGTAAAGGGCGTTGTACTT+TGG 0.501791 tig0022499:-98880 Msa1401110:CDS
TTATTAATAACAGCAGGAAA+AGG 0.523670 tig0022499:+98804 None:intergenic
TCTTCATTATTAATAACAGC+AGG 0.533828 tig0022499:+98798 None:intergenic
GTGTTTGTCTGATTCAGTCA+AGG 0.558037 tig0022499:+98746 None:intergenic
CTACAAAGAGGTCCAGTGAA+AGG 0.579253 tig0022499:-98939 Msa1401110:CDS
CAACGCCCTTTACTGCGACC+AGG 0.595863 tig0022499:+98888 None:intergenic
CGACCAGGAACTGTAGAGCA+TGG 0.600664 tig0022499:+98903 None:intergenic
TTAATAACAGCAGGAAAAGG+TGG 0.612990 tig0022499:+98807 None:intergenic
GCAAGAACACTACTACAAAG+AGG 0.639636 tig0022499:-98951 Msa1401110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTATTAATAACAGCAGGAAA+AGG + tig0022499:98962-98981 None:intergenic 25.0%
! TCTTCATTATTAATAACAGC+AGG + tig0022499:98968-98987 None:intergenic 25.0%
!!! TCTTGATCATTGCTTTTTTT+TGG + tig0022499:98997-99016 None:intergenic 25.0%
TTAATAACAGCAGGAAAAGG+TGG + tig0022499:98959-98978 None:intergenic 35.0%
GCAAGAACACTACTACAAAG+AGG - tig0022499:98812-98831 Msa1401110:CDS 40.0%
TTGGCTGAAATTCATGTTGC+TGG - tig0022499:98902-98921 Msa1401110:CDS 40.0%
GTGTTTGTCTGATTCAGTCA+AGG + tig0022499:99020-99039 None:intergenic 40.0%
CTACAAAGAGGTCCAGTGAA+AGG - tig0022499:98824-98843 Msa1401110:CDS 45.0%
TTCTTCGTCGAACCTTTCAC+TGG + tig0022499:98839-98858 None:intergenic 45.0%
AATCCATGCTCTACAGTTCC+TGG - tig0022499:98857-98876 Msa1401110:CDS 45.0%
!! CAGTAAAGGGCGTTGTACTT+TGG - tig0022499:98883-98902 Msa1401110:CDS 45.0%
ACAGTTCCTGGTCGCAGTAA+AGG - tig0022499:98869-98888 Msa1401110:CDS 50.0%
CAGTTCCTGGTCGCAGTAAA+GGG - tig0022499:98870-98889 Msa1401110:CDS 50.0%
CGACCAGGAACTGTAGAGCA+TGG + tig0022499:98863-98882 None:intergenic 55.0%
CAACGCCCTTTACTGCGACC+AGG + tig0022499:98878-98897 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0022499 gene 98728 99057 98728 ID=Msa1401110;Name=Msa1401110
tig0022499 mRNA 98728 99057 98728 ID=Msa1401110-mRNA-1;Parent=Msa1401110;Name=Msa1401110-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|109
tig0022499 exon 98728 99057 98728 ID=Msa1401110-mRNA-1:exon:18096;Parent=Msa1401110-mRNA-1
tig0022499 CDS 98728 99057 98728 ID=Msa1401110-mRNA-1:cds;Parent=Msa1401110-mRNA-1
Gene Sequence

>Msa1401110

ATGGGTAGCTACATGATAAAGAAGTTGTGCTTGTTGACAACATTAATAATAATTCTTGTATTGAGTATAGAGAACACAAGTGCAGCAAGAACACTACTACAAAGAGGTCCAGTGAAAGGTTCGACGAAGAATCCATGCTCTACAGTTCCTGGTCGCAGTAAAGGGCGTTGTACTTTGGCTGAAATTCATGTTGCTGGACATATTAACGTCGTTGCTCATTCATCATCACCACCTTTTCCTGCTGTTATTAATAATGAAGATAGCATCCAAAAAAAAGCAATGATCAAGACCTTGACTGAATCAGACAAACACCTACAATCTCAAAAATGA

Protein sequence

>Msa1401110

MGSYMIKKLCLLTTLIIILVLSIENTSAARTLLQRGPVKGSTKNPCSTVPGRSKGRCTLAEIHVAGHINVVAHSSSPPFPAVINNEDSIQKKAMIKTLTESDKHLQSQK*