AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0262360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0262360 MtrunA17_Chr2g0324911 100.000 78 0 0 1 78 1 78 1.41e-49 150
Msa0262360 MtrunA17_Chr2g0283451 60.811 74 29 0 5 78 7 80 6.04e-28 95.9
Msa0262360 MtrunA17_Chr4g0040991 58.974 78 31 1 1 78 1 77 5.56e-27 93.6
Msa0262360 MtrunA17_Chr2g0325441 53.846 78 35 1 1 78 1 77 1.35e-25 90.1
Msa0262360 MtrunA17_Chr4g0040491 72.727 77 21 0 2 78 3 79 4.26e-23 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0262360 AT2G20562.1 73.239 71 19 0 8 78 3 73 1.60e-25 90.1
Msa0262360 AT2G31090.1 59.155 71 29 0 8 78 5 75 5.04e-21 79.0

Find 33 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCGCTGGATTGGCAAATT+TGG 0.128393 2_2:+61229673 Msa0262360:CDS
CTCTTGTTGGTGCAGTTATC+TGG 0.152454 2_2:+61229290 Msa0262360:CDS
TCTCCACTTCCCCTCGTTCT+TGG 0.245405 2_2:+61229124 Msa0262360:five_prime_UTR
CGAGGGGAAGTGGAGAAGTT+AGG 0.288412 2_2:-61229117 None:intergenic
AGAAAGAAAGAAAGAAAATA+TGG 0.303892 2_2:+61229189 Msa0262360:exon
GAGGGGAAGTGGAGAAGTTA+GGG 0.336170 2_2:-61229116 None:intergenic
TTTCTTTCTATATTCTTCAA+TGG 0.404934 2_2:-61229163 None:intergenic
CAAATTCAAAGAAAACAAAA+AGG 0.405497 2_2:-61229815 None:intergenic
CCATGTTGCATTTGCTGCGC+TGG 0.414638 2_2:+61229659 Msa0262360:CDS
TTGCATTTGCTGCGCTGGAT+TGG 0.438326 2_2:+61229664 Msa0262360:CDS
ATCAAAGCCAAGAGAGCAAA+AGG 0.457758 2_2:-61229262 None:intergenic
GTAAATATCGTAAACTATGA+AGG 0.463185 2_2:-61229772 None:intergenic
TTCCAGTTCGTGTTCTTAAG+TGG 0.471610 2_2:+61229714 Msa0262360:CDS
AACCACTTAAGAACACGAAC+TGG 0.473947 2_2:-61229716 None:intergenic
TGGATTGGCAAATTTGGCTG+TGG 0.481790 2_2:+61229679 Msa0262360:CDS
GGTTGTGAATGCAGGCCATT+GGG 0.491219 2_2:+61229223 Msa0262360:CDS
TGACCAAGAACGAGGGGAAG+TGG 0.494668 2_2:-61229127 None:intergenic
GAAAATATGGCAGATGATGA+AGG 0.507098 2_2:+61229202 Msa0262360:exon
TAAATATCGTAAACTATGAA+GGG 0.517114 2_2:-61229771 None:intergenic
ATGATGAAGGTTGTGAATGC+AGG 0.535019 2_2:+61229215 Msa0262360:CDS
AATCAAAATATATAGACAAA+CGG 0.544954 2_2:-61229880 None:intergenic
GGATTGGCAAATTTGGCTGT+GGG 0.548115 2_2:+61229680 Msa0262360:CDS
CCAGCGCAGCAAATGCAACA+TGG 0.552638 2_2:-61229659 None:intergenic
TTATTTGTATCATGATTCAT+CGG 0.553612 2_2:+61229839 Msa0262360:three_prime_UTR
AGGTTGTGAATGCAGGCCAT+TGG 0.583286 2_2:+61229222 Msa0262360:CDS
CAGCGCAGCAAATGCAACAT+GGG 0.600667 2_2:-61229658 None:intergenic
AGGGGAAGTGGAGAAGTTAG+GGG 0.612620 2_2:-61229115 None:intergenic
AGGGGTCTTCATCTCTAACA+AGG 0.633868 2_2:-61229752 None:intergenic
AAATATCGTAAACTATGAAG+GGG 0.645560 2_2:-61229770 None:intergenic
GGGGAAGTGGAGAAGTTAGG+GGG 0.647330 2_2:-61229114 None:intergenic
TGTGAACGTGACCAAGAACG+AGG 0.653521 2_2:-61229135 None:intergenic
GTGAACGTGACCAAGAACGA+GGG 0.665471 2_2:-61229134 None:intergenic
TGAACGTGACCAAGAACGAG+GGG 0.695224 2_2:-61229133 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTATTTTATTATTTTT+TGG + chr2_2:61229571-61229590 Msa0262360:intron 0.0%
!!! TATTTTATTTTATTATTTTT+TGG + chr2_2:61229571-61229590 Msa0262360:intron 0.0%
!!! TTTATTATTTTTTGGTTTTT+TGG + chr2_2:61229579-61229598 Msa0262360:intron 10.0%
!!! TTTATTATTTTTTGGTTTTT+TGG + chr2_2:61229579-61229598 Msa0262360:intron 10.0%
!! ATATGATTTCATAAAATAAC+AGG - chr2_2:61229394-61229413 None:intergenic 15.0%
!! AATCAAAATATATAGACAAA+CGG - chr2_2:61229883-61229902 None:intergenic 15.0%
!! ATATGATTTCATAAAATAAC+AGG - chr2_2:61229394-61229413 None:intergenic 15.0%
!! AATCAAAATATATAGACAAA+CGG - chr2_2:61229883-61229902 None:intergenic 15.0%
!! TTTCTTTCTATATTCTTCAA+TGG - chr2_2:61229166-61229185 None:intergenic 20.0%
!! AGAAAGAAAGAAAGAAAATA+TGG + chr2_2:61229189-61229208 Msa0262360:exon 20.0%
!! AAAGATAAAACTTGATATCT+AGG + chr2_2:61229461-61229480 Msa0262360:intron 20.0%
!! AAGATAAAACTTGATATCTA+GGG + chr2_2:61229462-61229481 Msa0262360:intron 20.0%
!! TAAATATCGTAAACTATGAA+GGG - chr2_2:61229774-61229793 None:intergenic 20.0%
!! CAAATTCAAAGAAAACAAAA+AGG - chr2_2:61229818-61229837 None:intergenic 20.0%
!! TTATTTGTATCATGATTCAT+CGG + chr2_2:61229839-61229858 Msa0262360:three_prime_UTR 20.0%
!! TTTCTTTCTATATTCTTCAA+TGG - chr2_2:61229166-61229185 None:intergenic 20.0%
!! AGAAAGAAAGAAAGAAAATA+TGG + chr2_2:61229189-61229208 Msa0262360:exon 20.0%
!! AAAGATAAAACTTGATATCT+AGG + chr2_2:61229461-61229480 Msa0262360:intron 20.0%
!! AAGATAAAACTTGATATCTA+GGG + chr2_2:61229462-61229481 Msa0262360:intron 20.0%
!! TAAATATCGTAAACTATGAA+GGG - chr2_2:61229774-61229793 None:intergenic 20.0%
!! CAAATTCAAAGAAAACAAAA+AGG - chr2_2:61229818-61229837 None:intergenic 20.0%
!! TTATTTGTATCATGATTCAT+CGG + chr2_2:61229839-61229858 Msa0262360:three_prime_UTR 20.0%
! AAATATCGTAAACTATGAAG+GGG - chr2_2:61229773-61229792 None:intergenic 25.0%
! GTAAATATCGTAAACTATGA+AGG - chr2_2:61229775-61229794 None:intergenic 25.0%
! AAATATCGTAAACTATGAAG+GGG - chr2_2:61229773-61229792 None:intergenic 25.0%
! GTAAATATCGTAAACTATGA+AGG - chr2_2:61229775-61229794 None:intergenic 25.0%
!! GCTTTGATTTTATCTCTTGT+TGG + chr2_2:61229277-61229296 Msa0262360:CDS 30.0%
!! GCTTTGATTTTATCTCTTGT+TGG + chr2_2:61229277-61229296 Msa0262360:CDS 30.0%
GAAAATATGGCAGATGATGA+AGG + chr2_2:61229202-61229221 Msa0262360:exon 35.0%
AATCCAAGCAAAAAACCCAA+TGG - chr2_2:61229241-61229260 None:intergenic 35.0%
! GTGCAGTTATCTGGATTTTT+GGG + chr2_2:61229299-61229318 Msa0262360:CDS 35.0%
GAAAATATGGCAGATGATGA+AGG + chr2_2:61229202-61229221 Msa0262360:exon 35.0%
AATCCAAGCAAAAAACCCAA+TGG - chr2_2:61229241-61229260 None:intergenic 35.0%
! GTGCAGTTATCTGGATTTTT+GGG + chr2_2:61229299-61229318 Msa0262360:CDS 35.0%
ATGATGAAGGTTGTGAATGC+AGG + chr2_2:61229215-61229234 Msa0262360:CDS 40.0%
! TGGATTACCTTTTGCTCTCT+TGG + chr2_2:61229255-61229274 Msa0262360:CDS 40.0%
ATCAAAGCCAAGAGAGCAAA+AGG - chr2_2:61229265-61229284 None:intergenic 40.0%
! GGTGCAGTTATCTGGATTTT+TGG + chr2_2:61229298-61229317 Msa0262360:CDS 40.0%
TTCCAGTTCGTGTTCTTAAG+TGG + chr2_2:61229714-61229733 Msa0262360:CDS 40.0%
AACCACTTAAGAACACGAAC+TGG - chr2_2:61229719-61229738 None:intergenic 40.0%
ATGATGAAGGTTGTGAATGC+AGG + chr2_2:61229215-61229234 Msa0262360:CDS 40.0%
! TGGATTACCTTTTGCTCTCT+TGG + chr2_2:61229255-61229274 Msa0262360:CDS 40.0%
ATCAAAGCCAAGAGAGCAAA+AGG - chr2_2:61229265-61229284 None:intergenic 40.0%
! GGTGCAGTTATCTGGATTTT+TGG + chr2_2:61229298-61229317 Msa0262360:CDS 40.0%
TTCCAGTTCGTGTTCTTAAG+TGG + chr2_2:61229714-61229733 Msa0262360:CDS 40.0%
AACCACTTAAGAACACGAAC+TGG - chr2_2:61229719-61229738 None:intergenic 40.0%
!!! AGGCCATTGGGTTTTTTGCT+TGG + chr2_2:61229235-61229254 Msa0262360:CDS 45.0%
CTCTTGTTGGTGCAGTTATC+TGG + chr2_2:61229290-61229309 Msa0262360:CDS 45.0%
TGCGCATCAACTACTTTCAC+CGG + chr2_2:61229505-61229524 Msa0262360:intron 45.0%
!! TGGATTGGCAAATTTGGCTG+TGG + chr2_2:61229679-61229698 Msa0262360:CDS 45.0%
! GGATTGGCAAATTTGGCTGT+GGG + chr2_2:61229680-61229699 Msa0262360:CDS 45.0%
AGGGGTCTTCATCTCTAACA+AGG - chr2_2:61229755-61229774 None:intergenic 45.0%
!!! AGGCCATTGGGTTTTTTGCT+TGG + chr2_2:61229235-61229254 Msa0262360:CDS 45.0%
CTCTTGTTGGTGCAGTTATC+TGG + chr2_2:61229290-61229309 Msa0262360:CDS 45.0%
TGCGCATCAACTACTTTCAC+CGG + chr2_2:61229505-61229524 Msa0262360:intron 45.0%
!! TGGATTGGCAAATTTGGCTG+TGG + chr2_2:61229679-61229698 Msa0262360:CDS 45.0%
! GGATTGGCAAATTTGGCTGT+GGG + chr2_2:61229680-61229699 Msa0262360:CDS 45.0%
AGGGGTCTTCATCTCTAACA+AGG - chr2_2:61229755-61229774 None:intergenic 45.0%
AGGGGAAGTGGAGAAGTTAG+GGG - chr2_2:61229118-61229137 None:intergenic 50.0%
GAGGGGAAGTGGAGAAGTTA+GGG - chr2_2:61229119-61229138 None:intergenic 50.0%
TGAACGTGACCAAGAACGAG+GGG - chr2_2:61229136-61229155 None:intergenic 50.0%
GTGAACGTGACCAAGAACGA+GGG - chr2_2:61229137-61229156 None:intergenic 50.0%
TGTGAACGTGACCAAGAACG+AGG - chr2_2:61229138-61229157 None:intergenic 50.0%
AGGTTGTGAATGCAGGCCAT+TGG + chr2_2:61229222-61229241 Msa0262360:CDS 50.0%
GGTTGTGAATGCAGGCCATT+GGG + chr2_2:61229223-61229242 Msa0262360:CDS 50.0%
CAGCGCAGCAAATGCAACAT+GGG - chr2_2:61229661-61229680 None:intergenic 50.0%
! TTGCATTTGCTGCGCTGGAT+TGG + chr2_2:61229664-61229683 Msa0262360:CDS 50.0%
!! CTGCGCTGGATTGGCAAATT+TGG + chr2_2:61229673-61229692 Msa0262360:CDS 50.0%
AGGGGAAGTGGAGAAGTTAG+GGG - chr2_2:61229118-61229137 None:intergenic 50.0%
GAGGGGAAGTGGAGAAGTTA+GGG - chr2_2:61229119-61229138 None:intergenic 50.0%
TGAACGTGACCAAGAACGAG+GGG - chr2_2:61229136-61229155 None:intergenic 50.0%
GTGAACGTGACCAAGAACGA+GGG - chr2_2:61229137-61229156 None:intergenic 50.0%
TGTGAACGTGACCAAGAACG+AGG - chr2_2:61229138-61229157 None:intergenic 50.0%
AGGTTGTGAATGCAGGCCAT+TGG + chr2_2:61229222-61229241 Msa0262360:CDS 50.0%
GGTTGTGAATGCAGGCCATT+GGG + chr2_2:61229223-61229242 Msa0262360:CDS 50.0%
CAGCGCAGCAAATGCAACAT+GGG - chr2_2:61229661-61229680 None:intergenic 50.0%
! TTGCATTTGCTGCGCTGGAT+TGG + chr2_2:61229664-61229683 Msa0262360:CDS 50.0%
!! CTGCGCTGGATTGGCAAATT+TGG + chr2_2:61229673-61229692 Msa0262360:CDS 50.0%
GGGGAAGTGGAGAAGTTAGG+GGG - chr2_2:61229117-61229136 None:intergenic 55.0%
CGAGGGGAAGTGGAGAAGTT+AGG - chr2_2:61229120-61229139 None:intergenic 55.0%
TCTCCACTTCCCCTCGTTCT+TGG + chr2_2:61229124-61229143 Msa0262360:five_prime_UTR 55.0%
TGACCAAGAACGAGGGGAAG+TGG - chr2_2:61229130-61229149 None:intergenic 55.0%
ACTACTTTCACCGGACGCAG+CGG + chr2_2:61229514-61229533 Msa0262360:intron 55.0%
CCAGCGCAGCAAATGCAACA+TGG - chr2_2:61229662-61229681 None:intergenic 55.0%
! CCATGTTGCATTTGCTGCGC+TGG + chr2_2:61229659-61229678 Msa0262360:CDS 55.0%
GGGGAAGTGGAGAAGTTAGG+GGG - chr2_2:61229117-61229136 None:intergenic 55.0%
CGAGGGGAAGTGGAGAAGTT+AGG - chr2_2:61229120-61229139 None:intergenic 55.0%
TCTCCACTTCCCCTCGTTCT+TGG + chr2_2:61229124-61229143 Msa0262360:five_prime_UTR 55.0%
TGACCAAGAACGAGGGGAAG+TGG - chr2_2:61229130-61229149 None:intergenic 55.0%
ACTACTTTCACCGGACGCAG+CGG + chr2_2:61229514-61229533 Msa0262360:intron 55.0%
CCAGCGCAGCAAATGCAACA+TGG - chr2_2:61229662-61229681 None:intergenic 55.0%
! CCATGTTGCATTTGCTGCGC+TGG + chr2_2:61229659-61229678 Msa0262360:CDS 55.0%
! TGACTAAGCACCGCTGCGTC+CGG - chr2_2:61229527-61229546 None:intergenic 60.0%
! TGACTAAGCACCGCTGCGTC+CGG - chr2_2:61229527-61229546 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 61229052 61229906 61229052 ID=Msa0262360;Name=Msa0262360
chr2_2 mRNA 61229052 61229906 61229052 ID=Msa0262360-mRNA-1;Parent=Msa0262360;Name=Msa0262360-mRNA-1;_AED=0.01;_eAED=0.01;_QI=156|1|1|1|1|1|2|146|78
chr2_2 exon 61229052 61229320 61229052 ID=Msa0262360-mRNA-1:exon:18855;Parent=Msa0262360-mRNA-1
chr2_2 exon 61229637 61229906 61229637 ID=Msa0262360-mRNA-1:exon:18856;Parent=Msa0262360-mRNA-1
chr2_2 five_prime_UTR 61229052 61229207 61229052 ID=Msa0262360-mRNA-1:five_prime_utr;Parent=Msa0262360-mRNA-1
chr2_2 CDS 61229208 61229320 61229208 ID=Msa0262360-mRNA-1:cds;Parent=Msa0262360-mRNA-1
chr2_2 CDS 61229637 61229760 61229637 ID=Msa0262360-mRNA-1:cds;Parent=Msa0262360-mRNA-1
chr2_2 three_prime_UTR 61229761 61229906 61229761 ID=Msa0262360-mRNA-1:three_prime_utr;Parent=Msa0262360-mRNA-1
Gene Sequence

>Msa0262360

ATGGCAGATGATGAAGGTTGTGAATGCAGGCCATTGGGTTTTTTGCTTGGATTACCTTTTGCTCTCTTGGCTTTGATTTTATCTCTTGTTGGTGCAGTTATCTGGATTTTTGGATCTATATTGAGTTGTTTGTGCCCATGTTGCATTTGCTGCGCTGGATTGGCAAATTTGGCTGTGGGTCTTGTAAAGCTTCCAGTTCGTGTTCTTAAGTGGTTTACTCGACAAATTCCTTGTTAG

Protein sequence

>Msa0262360

MADDEGCECRPLGFLLGLPFALLALILSLVGAVIWIFGSILSCLCPCCICCAGLANLAVGLVKLPVRVLKWFTRQIPC*