AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0338010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0338010 MtrunA17_Chr2g0325481 97.826 368 5 1 1 365 1 368 0.0 751
Msa0338010 MtrunA17_Chr4g0041011 75.000 360 85 1 4 363 9 363 0.0 584
Msa0338010 MtrunA17_Chr2g0283581 59.563 366 137 5 1 363 1 358 2.07e-159 451
Msa0338010 MtrunA17_Chr4g0045951 55.890 365 158 2 1 364 1 363 1.23e-152 434
Msa0338010 MtrunA17_Chr4g0045941 55.191 366 147 3 1 364 1 351 8.73e-149 424
Msa0338010 MtrunA17_Chr3g0097891 54.829 321 142 2 47 364 31 351 1.63e-133 385
Msa0338010 MtrunA17_Chr2g0314801 55.799 319 137 3 46 360 32 350 2.28e-131 380
Msa0338010 MtrunA17_Chr3g0085071 52.905 327 153 1 38 363 37 363 2.29e-130 377
Msa0338010 MtrunA17_Chr3g0091031 50.448 335 165 1 30 363 14 348 2.24e-127 369
Msa0338010 MtrunA17_Chr3g0085081 52.201 318 151 1 47 363 31 348 1.05e-125 365
Msa0338010 MtrunA17_Chr5g0413851 51.796 334 155 4 35 364 29 360 6.61e-123 358
Msa0338010 MtrunA17_Chr3g0105301 44.800 375 190 4 7 364 3 377 4.35e-116 342
Msa0338010 MtrunA17_Chr5g0430371 44.236 373 167 5 17 364 44 400 4.12e-115 340
Msa0338010 MtrunA17_Chr2g0314791 45.289 329 158 8 47 364 29 346 3.24e-101 303
Msa0338010 MtrunA17_Chr1g0171171 42.941 340 170 8 39 363 45 375 1.07e-95 290
Msa0338010 MtrunA17_Chr1g0210771 38.040 347 194 6 31 363 43 382 1.31e-91 279
Msa0338010 MtrunA17_Chr1g0175671 38.400 375 204 6 15 364 138 510 2.79e-90 280
Msa0338010 MtrunA17_Chr7g0258851 40.233 343 180 6 46 364 247 588 1.85e-89 280
Msa0338010 MtrunA17_Chr4g0071391 37.318 343 186 6 46 363 108 446 4.09e-84 262
Msa0338010 MtrunA17_Chr5g0413271 34.637 358 217 4 23 364 121 477 1.00e-81 257
Msa0338010 MtrunA17_Chr3g0113671 39.017 346 184 9 47 365 106 451 1.48e-81 256
Msa0338010 MtrunA17_Chr4g0068111 38.441 372 198 10 23 364 84 454 2.10e-81 256
Msa0338010 MtrunA17_Chr7g0269991 39.759 332 176 8 47 359 137 463 2.73e-80 253
Msa0338010 MtrunA17_Chr1g0213281 36.827 353 193 8 39 364 167 516 1.27e-78 250
Msa0338010 MtrunA17_Chr5g0437291 38.109 349 186 10 47 365 165 513 1.28e-78 250
Msa0338010 MtrunA17_Chr8g0368461 36.667 360 186 7 43 364 174 529 2.05e-77 248
Msa0338010 MtrunA17_Chr3g0114101 34.085 399 227 10 2 364 33 431 8.08e-76 240
Msa0338010 MtrunA17_Chr5g0437601 37.069 348 194 7 42 364 78 425 3.15e-75 239
Msa0338010 MtrunA17_Chr4g0006991 34.555 382 208 10 24 363 46 427 1.20e-74 237
Msa0338010 MtrunA17_Chr8g0360521 35.613 351 196 9 40 364 64 410 4.34e-71 228
Msa0338010 MtrunA17_Chr1g0209921 48.261 230 110 7 44 268 41 266 2.19e-69 218
Msa0338010 MtrunA17_Chr2g0287901 37.059 340 188 7 47 361 97 435 7.84e-69 223
Msa0338010 MtrunA17_Chr7g0259711 36.812 345 189 8 47 364 91 433 3.28e-68 221
Msa0338010 MtrunA17_Chr4g0005481 35.632 348 198 9 36 359 97 442 3.33e-68 221
Msa0338010 MtrunA17_Chr4g0005491 32.687 361 209 9 33 364 53 408 5.48e-67 217
Msa0338010 MtrunA17_Chr7g0243301 31.969 391 230 10 4 365 28 411 1.12e-66 216
Msa0338010 MtrunA17_Chr7g0242701 35.838 346 195 9 44 363 83 427 4.79e-66 215
Msa0338010 MtrunA17_Chr3g0126021 36.202 337 191 7 47 364 129 460 3.00e-62 206
Msa0338010 MtrunA17_Chr8g0384151 30.029 343 220 6 37 364 80 417 2.61e-53 182
Msa0338010 MtrunA17_Chr1g0178081 31.778 343 154 10 47 364 55 342 3.65e-50 171
Msa0338010 MtrunA17_Chr6g0456691 28.753 393 232 11 4 363 42 419 6.03e-39 144
Msa0338010 MtrunA17_Chr5g0414051 29.592 392 221 16 9 359 39 416 5.65e-38 141
Msa0338010 MtrunA17_Chr5g0414061 30.049 406 224 19 4 359 39 434 5.68e-38 141
Msa0338010 MtrunA17_Chr1g0188191 28.792 389 230 13 12 359 195 577 6.54e-38 143
Msa0338010 MtrunA17_Chr6g0476041 27.895 380 232 11 16 359 137 510 9.92e-37 139
Msa0338010 MtrunA17_Chr6g0456681 29.040 396 228 14 3 359 23 404 1.77e-34 131
Msa0338010 MtrunA17_Chr6g0455901 30.725 345 204 12 47 359 71 412 2.67e-34 130
Msa0338010 MtrunA17_Chr8g0384421 31.389 360 206 17 35 363 123 472 2.67e-34 131
Msa0338010 MtrunA17_Chr5g0414091 30.636 346 204 11 47 359 85 427 5.23e-34 130
Msa0338010 MtrunA17_Chr8g0347321 27.225 382 226 11 20 363 72 439 5.46e-33 127
Msa0338010 MtrunA17_Chr2g0283851 28.940 349 203 13 48 359 56 396 5.88e-32 124
Msa0338010 MtrunA17_Chr5g0414041 29.480 346 208 12 47 359 87 429 8.84e-32 124
Msa0338010 MtrunA17_Chr7g0226991 28.010 382 221 16 15 358 32 397 3.15e-31 122
Msa0338010 MtrunA17_Chr4g0049421 27.666 347 211 13 47 359 48 388 4.14e-30 119
Msa0338010 MtrunA17_Chr8g0391481 26.862 376 228 13 22 359 2 368 6.22e-30 118
Msa0338010 MtrunA17_Chr3g0085101 44.737 114 62 1 231 343 1 114 3.33e-29 109
Msa0338010 MtrunA17_Chr4g0070341 29.395 347 208 15 47 363 132 471 3.99e-29 117
Msa0338010 MtrunA17_Chr6g0451321 28.788 396 218 18 14 363 80 457 2.55e-28 115
Msa0338010 MtrunA17_Chr8g0387921 27.066 351 215 13 44 359 71 415 1.16e-27 112
Msa0338010 MtrunA17_Chr3g0140371 28.080 349 205 13 47 359 18 356 3.33e-27 110
Msa0338010 MtrunA17_Chr7g0221271 36.719 128 81 0 46 173 78 205 1.49e-22 94.4
Msa0338010 MtrunA17_Chr7g0221291 44.444 90 50 0 46 135 78 167 3.65e-22 92.0
Msa0338010 MtrunA17_Chr5g0429211 38.532 109 60 2 74 176 162 269 2.01e-17 81.6
Msa0338010 MtrunA17_Chr1g0209931 44.944 89 44 2 279 365 9 94 9.02e-17 75.1
Msa0338010 MtrunA17_Chr2g0287931 32.773 119 66 3 107 211 2 120 1.90e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0338010 AT2G42570.1 66.279 344 111 3 26 364 22 365 3.06e-180 504
Msa0338010 AT2G31110.2 62.928 321 118 1 44 363 42 362 8.63e-166 468
Msa0338010 AT1G29050.1 59.882 339 134 2 27 363 40 378 1.57e-157 447
Msa0338010 AT2G34070.1 59.692 325 129 2 41 363 59 383 2.28e-150 429
Msa0338010 AT3G14850.2 55.108 323 141 2 42 360 29 351 4.07e-131 379
Msa0338010 AT1G78710.1 50.153 327 157 4 42 364 33 357 3.04e-121 354
Msa0338010 AT2G30900.1 50.000 324 156 4 47 364 43 366 1.48e-119 350
Msa0338010 AT2G34070.2 60.643 249 96 2 117 363 1 249 1.89e-111 325
Msa0338010 AT5G58600.1 47.619 336 158 5 47 364 66 401 1.36e-110 329
Msa0338010 AT2G31110.3 61.927 218 83 0 44 261 42 259 7.96e-108 317
Msa0338010 AT2G30010.1 45.322 342 163 5 47 364 56 397 3.09e-105 315
Msa0338010 AT2G30010.2 45.322 342 163 5 47 364 99 440 3.22e-105 317
Msa0338010 AT2G31110.1 63.768 207 74 1 158 363 8 214 4.30e-104 305
Msa0338010 AT3G14850.1 55.242 248 107 2 117 360 1 248 2.40e-95 285
Msa0338010 AT3G54260.1 42.515 334 167 6 47 364 51 375 6.78e-92 280
Msa0338010 AT5G06700.1 42.274 343 171 7 47 364 255 595 4.45e-91 285
Msa0338010 AT3G12060.1 39.678 373 197 7 19 364 165 536 1.59e-89 280
Msa0338010 AT1G78710.2 41.896 327 131 5 42 364 26 297 1.41e-87 266
Msa0338010 AT2G30900.2 50.424 236 109 3 47 280 43 272 1.31e-84 258
Msa0338010 AT3G62390.1 38.544 371 201 9 19 364 104 472 1.83e-82 259
Msa0338010 AT5G58600.2 51.786 224 106 2 47 268 66 289 5.50e-82 252
Msa0338010 AT5G20590.1 38.571 350 184 9 41 364 136 480 7.70e-82 258
Msa0338010 AT5G49340.1 37.960 353 190 8 39 364 92 442 1.23e-80 254
Msa0338010 AT1G60790.1 38.211 369 194 10 26 364 169 533 3.88e-80 255
Msa0338010 AT3G55990.1 37.464 347 190 9 46 365 139 485 3.76e-77 246
Msa0338010 AT2G40150.1 35.977 353 193 8 46 365 70 422 8.64e-76 240
Msa0338010 AT3G11030.1 38.000 350 179 10 47 364 106 449 8.53e-75 239
Msa0338010 AT2G40160.2 35.817 349 192 9 47 364 77 424 3.44e-74 237
Msa0338010 AT2G40160.1 36.047 344 188 9 47 359 77 419 5.02e-74 236
Msa0338010 AT1G73140.1 36.544 353 194 8 38 364 58 406 4.32e-73 233
Msa0338010 AT5G19160.1 36.388 371 193 10 24 361 82 442 5.74e-73 234
Msa0338010 AT5G01360.2 35.756 344 195 7 46 363 35 378 5.80e-72 229
Msa0338010 AT5G01360.1 35.756 344 195 7 46 363 91 434 2.01e-71 229
Msa0338010 AT5G01620.3 34.402 343 198 8 42 359 113 453 1.04e-70 228
Msa0338010 AT5G01620.2 34.402 343 198 8 42 359 105 445 1.10e-70 228
Msa0338010 AT5G01620.1 34.402 343 198 8 42 359 105 445 1.10e-70 228
Msa0338010 AT2G40320.1 35.632 348 199 8 42 364 76 423 8.05e-70 225
Msa0338010 AT3G06080.2 36.550 342 189 7 47 361 103 443 1.23e-69 226
Msa0338010 AT5G06230.2 35.574 357 201 7 36 364 10 365 4.60e-68 219
Msa0338010 AT5G06230.1 35.574 357 201 7 36 364 51 406 1.11e-67 219
Msa0338010 AT3G11570.1 37.538 325 180 7 63 365 98 421 5.75e-67 218
Msa0338010 AT2G38320.1 33.333 351 204 9 44 364 55 405 3.83e-64 210
Msa0338010 AT1G48880.1 33.973 365 194 8 21 364 102 440 4.72e-62 205
Msa0338010 AT3G62390.2 38.258 264 145 6 26 271 111 374 3.07e-57 192
Msa0338010 AT5G20590.2 39.431 246 128 7 41 269 136 377 1.48e-55 186
Msa0338010 AT5G19160.2 36.923 260 144 4 24 269 82 335 9.10e-54 181
Msa0338010 AT2G38320.2 31.056 322 194 7 71 364 1 322 4.16e-53 179
Msa0338010 AT2G40160.3 35.470 234 139 4 47 268 77 310 3.17e-50 171
Msa0338010 AT3G06080.1 37.131 237 135 3 47 269 103 339 4.27e-49 169
Msa0338010 AT5G06230.3 35.827 254 143 4 36 269 51 304 4.40e-46 160
Msa0338010 AT5G51640.1 28.571 378 222 11 23 359 114 484 5.19e-40 148
Msa0338010 AT1G70230.1 29.513 349 198 11 47 363 79 411 6.06e-40 146
Msa0338010 AT2G14530.1 29.049 389 240 12 7 363 22 406 7.60e-40 146
Msa0338010 AT4G25360.1 27.179 390 225 11 16 359 144 520 7.51e-37 140
Msa0338010 AT4G25360.2 27.179 390 225 11 16 359 144 520 7.51e-37 140
Msa0338010 AT5G64020.1 29.494 356 208 12 47 363 57 408 5.21e-35 133
Msa0338010 AT5G64470.3 26.744 344 223 10 47 364 55 395 1.47e-34 131
Msa0338010 AT1G01430.1 29.301 372 215 12 32 365 88 449 1.34e-33 130
Msa0338010 AT5G64470.2 26.857 350 221 11 47 364 55 401 4.15e-33 127
Msa0338010 AT5G15890.1 28.065 367 219 12 25 359 165 518 1.47e-32 128
Msa0338010 AT5G15900.1 26.934 349 217 11 47 363 67 409 3.66e-32 125
Msa0338010 AT5G15900.2 26.934 349 217 11 47 363 86 428 4.97e-32 125
Msa0338010 AT2G37720.1 30.939 362 183 14 47 363 143 482 5.01e-32 125
Msa0338010 AT5G20680.6 28.226 372 220 14 23 363 102 457 2.15e-31 124
Msa0338010 AT3G28150.1 30.966 352 199 16 46 363 69 410 3.10e-31 122
Msa0338010 AT3G02440.2 26.513 347 212 12 47 359 135 472 4.95e-31 123
Msa0338010 AT3G02440.1 26.513 347 212 12 47 359 127 464 5.19e-31 123
Msa0338010 AT5G20680.2 28.226 372 220 14 23 363 178 533 5.27e-31 123
Msa0338010 AT5G20680.4 29.023 348 208 13 47 363 212 551 6.62e-31 123
Msa0338010 AT5G20680.1 29.023 348 208 13 47 363 212 551 6.62e-31 123
Msa0338010 AT5G20680.3 29.023 348 208 13 47 363 212 551 6.62e-31 123
Msa0338010 AT5G20680.5 29.023 348 208 13 47 363 212 551 6.62e-31 123
Msa0338010 AT4G11090.1 25.679 405 243 15 8 365 35 428 8.34e-29 116
Msa0338010 AT4G01080.1 27.901 362 214 12 33 359 77 426 2.82e-28 115
Msa0338010 AT4G23790.1 25.275 364 217 13 46 365 78 430 5.56e-23 99.8
Msa0338010 AT5G64470.4 26.378 254 166 8 47 282 55 305 5.25e-21 92.8
Msa0338010 AT5G64470.1 26.378 254 166 8 47 282 55 305 7.35e-21 92.4

Find 86 sgRNAs with CRISPR-Local

Find 297 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCATGCAGCTCTCTT+TGG 0.210145 2_4:+58155122 Msa0338010:CDS
TTTATTCCGCACACCATATT+TGG 0.231078 2_4:+58154013 Msa0338010:CDS
TTGTAATTGGATAATAAAAT+TGG 0.234987 2_4:+58155252 Msa0338010:three_prime_UTR
TATCTCCACAATAACATTTC+AGG 0.240854 2_4:+58150839 Msa0338010:CDS
ATTGTACCAAATACACTCTT+TGG 0.242280 2_4:+58155336 Msa0338010:intron
AGGATCGAAACCCAAGAAAA+TGG 0.260094 2_4:+58150117 Msa0338010:exon
AGAGAAACACAACCATTCTT+TGG 0.302957 2_4:+58154897 Msa0338010:CDS
CGGTCAAGGTCTACCAAATA+TGG 0.307611 2_4:-58154026 None:intergenic
GTTTGACAACTTGGGCTAGA+TGG 0.309964 2_4:+58154328 Msa0338010:CDS
CTTTGGTTGAAGAAGTGAAT+TGG 0.315943 2_4:+58155139 Msa0338010:exon
TAGCATGTATGATTCATTCT+TGG 0.316438 2_4:+58150778 Msa0338010:CDS
TGTAGTCATTGGTGTCTTCC+AGG 0.323483 2_4:+58155077 Msa0338010:CDS
CAAGCCATTGGTGTTCCTGC+TGG 0.351195 2_4:-58154930 None:intergenic
AGAAGAACATTCCAAGTATC+AGG 0.357411 2_4:-58155104 None:intergenic
TCTACAAAGGTTTGACAACT+TGG 0.371927 2_4:+58154319 Msa0338010:CDS
TTCCAGGACTTCCTGATACT+TGG 0.372897 2_4:+58155093 Msa0338010:CDS
CAATTCAATTGTCAAAAGTA+TGG 0.391925 2_4:+58150355 Msa0338010:CDS
TATTTCTGATACTGAGTATC+AGG 0.397938 2_4:-58150382 None:intergenic
ACCTTGACCGTGAGAACATT+GGG 0.399023 2_4:+58154039 Msa0338010:CDS
AGCATGTATGATTCATTCTT+GGG 0.407416 2_4:+58150779 Msa0338010:CDS
CTCATTACTACTACTGGAAA+TGG 0.409028 2_4:-58150233 None:intergenic
ATCCTCTTTGTTGAAGGAAA+AGG 0.418304 2_4:-58150099 None:intergenic
AAGAAGACCTTGGTTTGAGC+TGG 0.426020 2_4:-58154369 None:intergenic
GTAACTGGTTTCGTGGCAAA+TGG 0.428187 2_4:+58150275 Msa0338010:CDS
GTTTCGATCCTCTTTGTTGA+AGG 0.444404 2_4:-58150105 None:intergenic
CTCAGTATCAGAAATATAGA+TGG 0.444563 2_4:+58150389 Msa0338010:CDS
TGCCAACAGATTGTAGTCAT+TGG 0.444942 2_4:+58155066 Msa0338010:CDS
TTCCTATGGTAGAAAACAAT+GGG 0.449400 2_4:-58155201 None:intergenic
ACCATAGGAATTATTTAACT+TGG 0.452262 2_4:+58155214 Msa0338010:three_prime_UTR
AGCAGGAACACCAATGGCTT+GGG 0.461353 2_4:+58154932 Msa0338010:CDS
AACTCGCTGGAAGATGTAAC+TGG 0.464742 2_4:+58150260 Msa0338010:CDS
TACATGCTAGTGAATTGAAC+TGG 0.471439 2_4:-58150764 None:intergenic
TTTGACAACTTGGGCTAGAT+GGG 0.479154 2_4:+58154329 Msa0338010:CDS
TTCACTATCACCCAAGCCAT+TGG 0.480458 2_4:-58154942 None:intergenic
ATAACACTACTATACCCTTC+TGG 0.481855 2_4:-58155044 None:intergenic
CATTGGGCGTGTTCTAAAGT+TGG 0.484902 2_4:+58154055 Msa0338010:CDS
ATTGAATTGTGGATCTATGA+AGG 0.486450 2_4:-58150341 None:intergenic
TTCCAAGTATCAGGAAGTCC+TGG 0.489986 2_4:-58155095 None:intergenic
GGATCGAAACCCAAGAAAAT+GGG 0.490190 2_4:+58150118 Msa0338010:exon
GCCAAGTTAAATAATTCCTA+TGG 0.499275 2_4:-58155215 None:intergenic
GGTAGTTGTTGCTGTTGTGT+TGG 0.501527 2_4:-58150212 None:intergenic
CAGCAGGAACACCAATGGCT+TGG 0.502272 2_4:+58154931 Msa0338010:CDS
AAGCCATTGGTGTTCCTGCT+GGG 0.502968 2_4:-58154929 None:intergenic
CGGTTGTGCATTTCCAGTGT+GGG 0.509875 2_4:-58154147 None:intergenic
ATGGAGAGGAATGGATGTAT+TGG 0.512382 2_4:+58154100 Msa0338010:CDS
CTTTGTAGAACGCAATGAAA+CGG 0.518428 2_4:-58154305 None:intergenic
TAACTGGTTTCGTGGCAAAT+GGG 0.526407 2_4:+58150276 Msa0338010:CDS
CGTTTCATTGCGTTCTACAA+AGG 0.529142 2_4:+58154306 Msa0338010:CDS
GATAGCACTTTGCTTTGAGA+AGG 0.530854 2_4:-58150820 None:intergenic
CTACAAAGGTTTGACAACTT+GGG 0.543154 2_4:+58154320 Msa0338010:CDS
TGGCATTGGTGGACCCACAC+TGG 0.546946 2_4:+58154134 Msa0338010:CDS
GACCTTGACCGTGAGAACAT+TGG 0.550954 2_4:+58154038 Msa0338010:CDS
TCTATGAAGGGACAAGAAGA+AGG 0.551169 2_4:-58150328 None:intergenic
ATTCCTATGGTAGAAAACAA+TGG 0.552378 2_4:-58155202 None:intergenic
GGAAGATGTAACTGGTTTCG+TGG 0.558355 2_4:+58150268 Msa0338010:CDS
GAAAAGATGCACATCCAGAA+GGG 0.559145 2_4:+58155030 Msa0338010:CDS
TTTGGTTTGAAGTACCCAGC+AGG 0.566621 2_4:+58154915 Msa0338010:CDS
TGAAGAAGTGAATTGGAAAG+TGG 0.568982 2_4:+58155146 Msa0338010:exon
GGATCATAGAGAGGATACGA+AGG 0.570131 2_4:-58150307 None:intergenic
GGGTACTTCAAACCAAAGAA+TGG 0.571214 2_4:-58154909 None:intergenic
ATATGTTTCTTAATTAGTCG+AGG 0.572676 2_4:+58155174 Msa0338010:three_prime_UTR
CTTTCACTTGTTCTATACCA+AGG 0.575010 2_4:+58150416 Msa0338010:CDS
ACTAACCTGAAATGTTATTG+TGG 0.576103 2_4:-58150844 None:intergenic
CAATAAAGAGTGGTAATGCA+TGG 0.581612 2_4:+58154081 Msa0338010:CDS
ACGGTTGTGCATTTCCAGTG+TGG 0.583332 2_4:-58154148 None:intergenic
AGAAAAGATGCACATCCAGA+AGG 0.583703 2_4:+58155029 Msa0338010:CDS
TGGTAATGCATGGAGAGGAA+TGG 0.587257 2_4:+58154091 Msa0338010:CDS
CACCAATGACTACAATCTGT+TGG 0.588126 2_4:-58155068 None:intergenic
GCCCAATGTTCTCACGGTCA+AGG 0.590015 2_4:-58154040 None:intergenic
AAGAGTGGTAATGCATGGAG+AGG 0.592479 2_4:+58154086 Msa0338010:CDS
CAGATGGGATTATATTCAAG+AGG 0.594517 2_4:+58154257 Msa0338010:intron
ACAACTTACTCGTAATGCAC+AGG 0.597709 2_4:-58154405 None:intergenic
AGTAATGAGAGAAAACTCGC+TGG 0.602900 2_4:+58150247 Msa0338010:CDS
TTGAATTGTGGATCTATGAA+GGG 0.607685 2_4:-58150340 None:intergenic
TGTAAATCCAGCTCAAACCA+AGG 0.609530 2_4:+58154362 Msa0338010:CDS
CTTGGTATAGAACAAGTGAA+AGG 0.623660 2_4:-58150415 None:intergenic
GAACACGCCCAATGTTCTCA+CGG 0.633134 2_4:-58154046 None:intergenic
TTATATTCAAGAGGGTGGCA+AGG 0.635985 2_4:+58154266 Msa0338010:CDS
TGGGATTATATTCAAGAGGG+TGG 0.638991 2_4:+58154261 Msa0338010:CDS
CAAGAAGAAGGATCATAGAG+AGG 0.640153 2_4:-58150316 None:intergenic
GTACCCAGCAGGAACACCAA+TGG 0.661011 2_4:+58154926 Msa0338010:CDS
AGATGGGATTATATTCAAGA+GGG 0.662305 2_4:+58154258 Msa0338010:intron
GGTCTACCAAATATGGTGTG+CGG 0.672109 2_4:-58154019 None:intergenic
GATCATAGAGAGGATACGAA+GGG 0.672935 2_4:-58150306 None:intergenic
GTCACATCTAGAAAATACAC+TGG 0.675733 2_4:-58154990 None:intergenic
AAGTTGGACTCAATAAAGAG+TGG 0.679146 2_4:+58154071 Msa0338010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAAAATACATTTATTG+GGG + chr2_4:58151384-58151403 Msa0338010:intron 10.0%
!! ACTATATATTTATTCATTAT+AGG + chr2_4:58153365-58153384 Msa0338010:intron 10.0%
!!! TTATTATTTGAATTATTTTC+AGG + chr2_4:58153971-58153990 Msa0338010:intron 10.0%
!! TTTATCAACTATATTTATAA+TGG + chr2_4:58154815-58154834 Msa0338010:intron 10.0%
!!! TGTAAATTTTCATATTATTT+TGG + chr2_4:58155298-58155317 Msa0338010:three_prime_UTR 10.0%
!! ACAACAAAAACATTATAAAA+AGG + chr2_4:58150043-58150062 Msa0338010:five_prime_UTR 15.0%
!! TATCAAAAAATCGAAATTAA+AGG - chr2_4:58150895-58150914 None:intergenic 15.0%
!!! TTTGATACTTTTGAATTTAA+TGG + chr2_4:58150908-58150927 Msa0338010:intron 15.0%
!!! ATTTTTATTATTTTCCATGT+TGG + chr2_4:58151127-58151146 Msa0338010:intron 15.0%
!! TTAAAAAATACATTTATTGG+GGG + chr2_4:58151385-58151404 Msa0338010:intron 15.0%
!!! CATAATTTAAGTTTACATAT+AGG + chr2_4:58151933-58151952 Msa0338010:intron 15.0%
!!! TTCAATACAAATTTCATTTT+TGG - chr2_4:58152617-58152636 None:intergenic 15.0%
!! GTAAATAAATTATATGTTAG+TGG + chr2_4:58152738-58152757 Msa0338010:intron 15.0%
!! AGAATAATACAATATTAGTA+TGG + chr2_4:58152764-58152783 Msa0338010:intron 15.0%
!!! GATCATGAATATAATTTTAT+TGG - chr2_4:58152946-58152965 None:intergenic 15.0%
!! ACAAAAAATAAAATTTGAAG+TGG + chr2_4:58153304-58153323 Msa0338010:intron 15.0%
!!! CAAAAAATAAAATTTGAAGT+GGG + chr2_4:58153305-58153324 Msa0338010:intron 15.0%
!! AATATAAATTACTAGAAGAA+AGG - chr2_4:58153643-58153662 None:intergenic 15.0%
!! ATGTACAATTATTATATGTA+AGG + chr2_4:58153797-58153816 Msa0338010:intron 15.0%
!!! AATGTTTTTTTACAGAATTA+CGG + chr2_4:58154476-58154495 Msa0338010:intron 15.0%
!! ATAAAATTTAGTCTAGTAAT+TGG - chr2_4:58154800-58154819 None:intergenic 15.0%
!!! TTTTGAGTTATTATTGTAAT+TGG + chr2_4:58155239-58155258 Msa0338010:three_prime_UTR 15.0%
!! TTGTAATTGGATAATAAAAT+TGG + chr2_4:58155252-58155271 Msa0338010:three_prime_UTR 15.0%
!! TTAGAAAATGAAAATGTGTT+TGG + chr2_4:58150655-58150674 Msa0338010:intron 20.0%
!!! AATGTGTTTGGTATTTTTTT+AGG + chr2_4:58150667-58150686 Msa0338010:intron 20.0%
!!! AAATTATGTTGGTTATGTTT+TGG + chr2_4:58150936-58150955 Msa0338010:intron 20.0%
!! TTTGAAGAAAAACAAAACAT+GGG - chr2_4:58151052-58151071 None:intergenic 20.0%
!! ATTTGAAGAAAAACAAAACA+TGG - chr2_4:58151053-58151072 None:intergenic 20.0%
!! TTATGAAAAAACTATTACCA+TGG - chr2_4:58151281-58151300 None:intergenic 20.0%
!!! TTTTTTCATAAAAAATGCCA+TGG + chr2_4:58151290-58151309 Msa0338010:intron 20.0%
!! ATTACTATCTATTATTACCA+TGG - chr2_4:58151310-58151329 None:intergenic 20.0%
!! TAAAAAATACATTTATTGGG+GGG + chr2_4:58151386-58151405 Msa0338010:intron 20.0%
!!! TTTTAGAAATTTAGATGAAG+AGG - chr2_4:58151620-58151639 None:intergenic 20.0%
!! TCAGAATGATAATAAAAGAA+GGG - chr2_4:58151644-58151663 None:intergenic 20.0%
!! TTCAGAATGATAATAAAAGA+AGG - chr2_4:58151645-58151664 None:intergenic 20.0%
!! TAAAAAAAGATACAAACTAG+TGG + chr2_4:58152787-58152806 Msa0338010:intron 20.0%
!! TCCTAAAGTTTCTATATAAA+GGG - chr2_4:58152988-58153007 None:intergenic 20.0%
!! TTCCTAAAGTTTCTATATAA+AGG - chr2_4:58152989-58153008 None:intergenic 20.0%
!!! ATTAGCAATAATTAGTGTTT+TGG + chr2_4:58153020-58153039 Msa0338010:intron 20.0%
!! TGATCATTACAGTTTAAAAA+GGG - chr2_4:58153140-58153159 None:intergenic 20.0%
!! TTGATCATTACAGTTTAAAA+AGG - chr2_4:58153141-58153160 None:intergenic 20.0%
!! GCTATTCACATTAAAAAAAT+AGG + chr2_4:58153250-58153269 Msa0338010:intron 20.0%
!! GTACTACAATTTAATTCATA+TGG - chr2_4:58153331-58153350 None:intergenic 20.0%
!! TTCTAGTAATTTATATTCCA+TGG + chr2_4:58153646-58153665 Msa0338010:intron 20.0%
!!! TTGAATTATTTTCAGGATTA+TGG + chr2_4:58153978-58153997 Msa0338010:intron 20.0%
!! CATAATCAATTCTGTTATTA+AGG + chr2_4:58154195-58154214 Msa0338010:intron 20.0%
!!! TTTTAAATTTCAGTAGAATC+AGG + chr2_4:58154512-58154531 Msa0338010:intron 20.0%
!! TTTAAATTTCAGTAGAATCA+GGG + chr2_4:58154513-58154532 Msa0338010:intron 20.0%
!! AAAGAAATCATTAGAATGTT+TGG + chr2_4:58154601-58154620 Msa0338010:intron 20.0%
!! TTATAATGGTGTATGATATT+TGG + chr2_4:58154829-58154848 Msa0338010:intron 20.0%
!! TGTATGATATTTGGTAAATT+AGG + chr2_4:58154838-58154857 Msa0338010:intron 20.0%
!! GTATGATATTTGGTAAATTA+GGG + chr2_4:58154839-58154858 Msa0338010:intron 20.0%
!! TATGATATTTGGTAAATTAG+GGG + chr2_4:58154840-58154859 Msa0338010:intron 20.0%
!! TAAATTCAAGAGCTTTTGTT+TGG - chr2_4:58150189-58150208 None:intergenic 25.0%
!! TACTTTTGACAATTGAATTG+TGG - chr2_4:58150355-58150374 None:intergenic 25.0%
! CAATTCAATTGTCAAAAGTA+TGG + chr2_4:58150355-58150374 Msa0338010:CDS 25.0%
! TAAGTAGCTAGTATAATGTT+TGG - chr2_4:58150579-58150598 None:intergenic 25.0%
!!! TTTTTTTAGGTTCAATGCAT+TGG + chr2_4:58150680-58150699 Msa0338010:intron 25.0%
! GGAAAAAAGATTATGTTTGT+TGG + chr2_4:58150723-58150742 Msa0338010:CDS 25.0%
!! TAATGGTAGTGAAATTATGT+TGG + chr2_4:58150925-58150944 Msa0338010:intron 25.0%
! ACTCAATAAAGTTTCCAATT+AGG - chr2_4:58151013-58151032 None:intergenic 25.0%
!! TTATTCTTTAAAAATGTCCC+CGG - chr2_4:58151216-58151235 None:intergenic 25.0%
! TAATAAATGTGAATCTTCCA+TGG + chr2_4:58151233-58151252 Msa0338010:intron 25.0%
! GTAAATGATAGTAATAACCA+TGG - chr2_4:58151253-58151272 None:intergenic 25.0%
!! AACATATTTTCCTCTTACAA+AGG + chr2_4:58151344-58151363 Msa0338010:intron 25.0%
!! GTTTTTAAATCCTTTGTAAG+AGG - chr2_4:58151357-58151376 None:intergenic 25.0%
!! TTTCTTTTCCTGATCAAAAA+TGG + chr2_4:58151474-58151493 Msa0338010:intron 25.0%
!!! TAAATATGCCATTTTTGATC+AGG - chr2_4:58151485-58151504 None:intergenic 25.0%
!!! ATTCAATTGACGATTTTAGT+AGG - chr2_4:58151716-58151735 None:intergenic 25.0%
!! TTGATTTAAGTTTACGTAGA+AGG + chr2_4:58151906-58151925 Msa0338010:intron 25.0%
!! TGATTTAAGTTTACGTAGAA+GGG + chr2_4:58151907-58151926 Msa0338010:intron 25.0%
!! AAAGGGTATATTAGTAACTT+TGG + chr2_4:58152293-58152312 Msa0338010:intron 25.0%
!! AAGGGTATATTAGTAACTTT+GGG + chr2_4:58152294-58152313 Msa0338010:intron 25.0%
! ATAGAACACCAAATATAAGA+CGG - chr2_4:58152572-58152591 None:intergenic 25.0%
!!! AGAAAAAGGCACTATATATT+AGG - chr2_4:58152903-58152922 None:intergenic 25.0%
! AGTTTCTATATAAAGGGAAA+AGG - chr2_4:58152982-58153001 None:intergenic 25.0%
! TCCCTTTATATAGAAACTTT+AGG + chr2_4:58152984-58153003 Msa0338010:intron 25.0%
! TTACTACTAATGATGATTGT+TGG - chr2_4:58153066-58153085 None:intergenic 25.0%
! GATCATTACAGTTTAAAAAG+GGG - chr2_4:58153139-58153158 None:intergenic 25.0%
! TTGATTGATGCTTATATCAA+GGG - chr2_4:58153230-58153249 None:intergenic 25.0%
!! TTTTTGTTCTATTGAGAACA+AGG - chr2_4:58153291-58153310 None:intergenic 25.0%
!!! TTTGAAGCGATTATCTTTTT+TGG - chr2_4:58153487-58153506 None:intergenic 25.0%
! AAAAGATAATCGCTTCAAAA+TGG + chr2_4:58153488-58153507 Msa0338010:intron 25.0%
!!! ACAAAAGTGTCACAAAATAT+TGG + chr2_4:58153587-58153606 Msa0338010:intron 25.0%
!!! CAAAAGTGTCACAAAATATT+GGG + chr2_4:58153588-58153607 Msa0338010:intron 25.0%
!! TTCATTTTCATTGAAACTAG+AGG + chr2_4:58153674-58153693 Msa0338010:intron 25.0%
! AACAGAATTGATTATGATCA+TGG - chr2_4:58154191-58154210 None:intergenic 25.0%
! AATCAATTCTGTTATTAAGG+AGG + chr2_4:58154198-58154217 Msa0338010:intron 25.0%
!! TAAATTTTACAAATTCGTGG+TGG - chr2_4:58154659-58154678 None:intergenic 25.0%
!! CTCTAAATTTTACAAATTCG+TGG - chr2_4:58154662-58154681 None:intergenic 25.0%
!! TTAGTGAATGTTTTGTACTA+TGG - chr2_4:58154704-58154723 None:intergenic 25.0%
! ATGATATTTGGTAAATTAGG+GGG + chr2_4:58154841-58154860 Msa0338010:intron 25.0%
! ATATGTTTCTTAATTAGTCG+AGG + chr2_4:58155174-58155193 Msa0338010:three_prime_UTR 25.0%
! ACCATAGGAATTATTTAACT+TGG + chr2_4:58155214-58155233 Msa0338010:three_prime_UTR 25.0%
!! AATATTCCAAAGAGTGTATT+TGG - chr2_4:58155345-58155364 None:intergenic 25.0%
! AACAGAAAACCCATTTTCTT+GGG - chr2_4:58150130-58150149 None:intergenic 30.0%
! TAACAGAAAACCCATTTTCT+TGG - chr2_4:58150131-58150150 None:intergenic 30.0%
AGAAGAAGTAAAAACAGAGT+CGG - chr2_4:58150154-58150173 None:intergenic 30.0%
! TTTTCTCTCATTACTACTAC+TGG - chr2_4:58150242-58150261 None:intergenic 30.0%
TTGAATTGTGGATCTATGAA+GGG - chr2_4:58150343-58150362 None:intergenic 30.0%
ATTGAATTGTGGATCTATGA+AGG - chr2_4:58150344-58150363 None:intergenic 30.0%
! TATTTCTGATACTGAGTATC+AGG - chr2_4:58150385-58150404 None:intergenic 30.0%
CTCAGTATCAGAAATATAGA+TGG + chr2_4:58150389-58150408 Msa0338010:CDS 30.0%
AGAAGGAGATATATATACCT+TGG - chr2_4:58150436-58150455 None:intergenic 30.0%
AACAGAAGCTATGATAGAAA+TGG - chr2_4:58150459-58150478 None:intergenic 30.0%
TGTTAGATTTAGTGTAGGTT+TGG + chr2_4:58150475-58150494 Msa0338010:intron 30.0%
GAGTAAATCTATCAGAATCA+CGG - chr2_4:58150551-58150570 None:intergenic 30.0%
!! TGGATTTTCTAGCAAAGTAT+AGG + chr2_4:58150700-58150719 Msa0338010:CDS 30.0%
!! GGATTTTCTAGCAAAGTATA+GGG + chr2_4:58150701-58150720 Msa0338010:CDS 30.0%
!! GATTTTCTAGCAAAGTATAG+GGG + chr2_4:58150702-58150721 Msa0338010:CDS 30.0%
TAGCATGTATGATTCATTCT+TGG + chr2_4:58150778-58150797 Msa0338010:CDS 30.0%
AGCATGTATGATTCATTCTT+GGG + chr2_4:58150779-58150798 Msa0338010:CDS 30.0%
TATCTCCACAATAACATTTC+AGG + chr2_4:58150839-58150858 Msa0338010:CDS 30.0%
ACTAACCTGAAATGTTATTG+TGG - chr2_4:58150847-58150866 None:intergenic 30.0%
! ATCCATTTTGTAGTTACTAG+CGG + chr2_4:58151078-58151097 Msa0338010:intron 30.0%
GTGAGCAATAAAGTATGAAA+CGG + chr2_4:58151166-58151185 Msa0338010:intron 30.0%
TGAGCAATAAAGTATGAAAC+GGG + chr2_4:58151167-58151186 Msa0338010:intron 30.0%
! AATAAAGTATGAAACGGGTT+TGG + chr2_4:58151172-58151191 Msa0338010:intron 30.0%
AAAAACTATTACCATGGCAA+TGG - chr2_4:58151275-58151294 None:intergenic 30.0%
GGTGTAACTATGATTATATG+GGG - chr2_4:58151559-58151578 None:intergenic 30.0%
GGGTGTAACTATGATTATAT+GGG - chr2_4:58151560-58151579 None:intergenic 30.0%
TGGGTGTAACTATGATTATA+TGG - chr2_4:58151561-58151580 None:intergenic 30.0%
! TTAGATGAAGAGGACAATTT+TGG - chr2_4:58151610-58151629 None:intergenic 30.0%
ATTAATCGAGTACGTATCAA+TGG - chr2_4:58151750-58151769 None:intergenic 30.0%
! CGATGTTGTTAATTTTACCA+TGG + chr2_4:58151833-58151852 Msa0338010:intron 30.0%
ACTACAACTATAAACATCCA+TGG - chr2_4:58151853-58151872 None:intergenic 30.0%
AGGGTATATTAGTAACTTTG+GGG + chr2_4:58152295-58152314 Msa0338010:intron 30.0%
! GAAACACGTGTTTTCTTTTT+CGG - chr2_4:58152518-58152537 None:intergenic 30.0%
!!! GTTTTTTTTTGTTGCGAAAC+CGG - chr2_4:58152873-58152892 None:intergenic 30.0%
TCCTATGCAAAGAAAGAAAA+AGG - chr2_4:58152917-58152936 None:intergenic 30.0%
CTTGATTGATGCTTATATCA+AGG - chr2_4:58153231-58153250 None:intergenic 30.0%
!! GTAGTTTACTGACTTTATGT+AGG - chr2_4:58153569-58153588 None:intergenic 30.0%
!! AATGAAAATGAAAAGTGCCA+TGG - chr2_4:58153666-58153685 None:intergenic 30.0%
AGAAAATAGACATCACTGAA+AGG + chr2_4:58153698-58153717 Msa0338010:intron 30.0%
GTGTGCGGAATAAAAATAAT+TGG - chr2_4:58154007-58154026 None:intergenic 30.0%
!!! ATGTATTGGTTTTCAACACA+TGG + chr2_4:58154114-58154133 Msa0338010:CDS 30.0%
GGTGTTAAATATAGATCCTA+CGG - chr2_4:58154170-58154189 None:intergenic 30.0%
! TTTTGACATTTGAATGCAGA+TGG + chr2_4:58154241-58154260 Msa0338010:intron 30.0%
TTTGACATTTGAATGCAGAT+GGG + chr2_4:58154242-58154261 Msa0338010:intron 30.0%
AGATGGGATTATATTCAAGA+GGG + chr2_4:58154258-58154277 Msa0338010:intron 30.0%
AATTCCTAAAAAGAAGACCT+TGG - chr2_4:58154382-58154401 None:intergenic 30.0%
TTCCTATGGTAGAAAACAAT+GGG - chr2_4:58155204-58155223 None:intergenic 30.0%
ATTCCTATGGTAGAAAACAA+TGG - chr2_4:58155205-58155224 None:intergenic 30.0%
GCCAAGTTAAATAATTCCTA+TGG - chr2_4:58155218-58155237 None:intergenic 30.0%
ATTGTACCAAATACACTCTT+TGG + chr2_4:58155336-58155355 Msa0338010:intron 30.0%
ATCCTCTTTGTTGAAGGAAA+AGG - chr2_4:58150102-58150121 None:intergenic 35.0%
CTCATTACTACTACTGGAAA+TGG - chr2_4:58150236-58150255 None:intergenic 35.0%
CTTGGTATAGAACAAGTGAA+AGG - chr2_4:58150418-58150437 None:intergenic 35.0%
CTTTCACTTGTTCTATACCA+AGG + chr2_4:58150416-58150435 Msa0338010:CDS 35.0%
AGCTATGATAGAAATGGAGA+AGG - chr2_4:58150453-58150472 None:intergenic 35.0%
GCTTCTGTTAGATTTAGTGT+AGG + chr2_4:58150470-58150489 Msa0338010:intron 35.0%
TACATGCTAGTGAATTGAAC+TGG - chr2_4:58150767-58150786 None:intergenic 35.0%
!!! GAGAAGGAATACCTTGTTTT+TGG - chr2_4:58150807-58150826 None:intergenic 35.0%
CAATTAGGTGAAAGACAGAA+AGG - chr2_4:58150998-58151017 None:intergenic 35.0%
CTTTCTGTCTTTCACCTAAT+TGG + chr2_4:58150996-58151015 Msa0338010:intron 35.0%
ACTATCATTTACCATTGCCA+TGG + chr2_4:58151261-58151280 Msa0338010:intron 35.0%
ATTGTACTTTCCCTCTTCTT+TGG + chr2_4:58151438-58151457 Msa0338010:intron 35.0%
! TATTGATTGCATATCCATGG+TGG - chr2_4:58151693-58151712 None:intergenic 35.0%
!! AGGTATTGATTGCATATCCA+TGG - chr2_4:58151696-58151715 None:intergenic 35.0%
ACACCTAAACTCAGTTTACT+TGG + chr2_4:58152003-58152022 Msa0338010:intron 35.0%
ACACCAAGTAAACTGAGTTT+AGG - chr2_4:58152009-58152028 None:intergenic 35.0%
TGTGCAGTGAACATGAATAA+AGG + chr2_4:58152275-58152294 Msa0338010:intron 35.0%
GTGCAGTGAACATGAATAAA+GGG + chr2_4:58152276-58152295 Msa0338010:intron 35.0%
GGGTGTAACTTGGATTATTT+GGG + chr2_4:58152315-58152334 Msa0338010:intron 35.0%
GGTGTAACTTGGATTATTTG+GGG + chr2_4:58152316-58152335 Msa0338010:intron 35.0%
AACACCAAATATAAGACGGA+TGG - chr2_4:58152568-58152587 None:intergenic 35.0%
GAATAGAGCTTAATCGCTTA+AGG - chr2_4:58152851-58152870 None:intergenic 35.0%
TAAGCGATTAAGCTCTATTC+CGG + chr2_4:58152851-58152870 Msa0338010:intron 35.0%
! GCCTTTTTCTTTCTTTGCAT+AGG + chr2_4:58152913-58152932 Msa0338010:intron 35.0%
!! GGGAAAAGGTATTGACAATT+TGG - chr2_4:58152968-58152987 None:intergenic 35.0%
! CCTTCTTTTATCACACAATG+TGG - chr2_4:58153614-58153633 None:intergenic 35.0%
CCACATTGTGTGATAAAAGA+AGG + chr2_4:58153611-58153630 Msa0338010:intron 35.0%
TCTCAAGTAGTGTAGTACTT+TGG + chr2_4:58153738-58153757 Msa0338010:intron 35.0%
CAAATCACAATCAATCGTGT+TGG - chr2_4:58153903-58153922 None:intergenic 35.0%
ACACGATTGATTGTGATTTG+TGG + chr2_4:58153903-58153922 Msa0338010:intron 35.0%
ATACACTACATTGTTGACAC+TGG - chr2_4:58153933-58153952 None:intergenic 35.0%
TTTATTCCGCACACCATATT+TGG + chr2_4:58154013-58154032 Msa0338010:CDS 35.0%
AAGTTGGACTCAATAAAGAG+TGG + chr2_4:58154071-58154090 Msa0338010:CDS 35.0%
! CAATAAAGAGTGGTAATGCA+TGG + chr2_4:58154081-58154100 Msa0338010:CDS 35.0%
CAGATGGGATTATATTCAAG+AGG + chr2_4:58154257-58154276 Msa0338010:intron 35.0%
CTTTGTAGAACGCAATGAAA+CGG - chr2_4:58154308-58154327 None:intergenic 35.0%
TCTACAAAGGTTTGACAACT+TGG + chr2_4:58154319-58154338 Msa0338010:CDS 35.0%
CTACAAAGGTTTGACAACTT+GGG + chr2_4:58154320-58154339 Msa0338010:CDS 35.0%
! CAAACCAAGGTCTTCTTTTT+AGG + chr2_4:58154375-58154394 Msa0338010:CDS 35.0%
!! ACACATAGATTTTGGCATAC+TGG - chr2_4:58154635-58154654 None:intergenic 35.0%
! GTGAATGTTTTGTACTATGG+TGG - chr2_4:58154701-58154720 None:intergenic 35.0%
AGAGAAACACAACCATTCTT+TGG + chr2_4:58154897-58154916 Msa0338010:CDS 35.0%
GTCACATCTAGAAAATACAC+TGG - chr2_4:58154993-58155012 None:intergenic 35.0%
ATAACACTACTATACCCTTC+TGG - chr2_4:58155047-58155066 None:intergenic 35.0%
AGAAGAACATTCCAAGTATC+AGG - chr2_4:58155107-58155126 None:intergenic 35.0%
CTTTGGTTGAAGAAGTGAAT+TGG + chr2_4:58155139-58155158 Msa0338010:exon 35.0%
TGAAGAAGTGAATTGGAAAG+TGG + chr2_4:58155146-58155165 Msa0338010:exon 35.0%
! AACCCATTGTTTTCTACCAT+AGG + chr2_4:58155199-58155218 Msa0338010:three_prime_UTR 35.0%
GAGTGTATTTGGTACAATCA+AGG - chr2_4:58155334-58155353 None:intergenic 35.0%
!! GACAACTTTTTCTGCACTCT+TGG + chr2_4:58150075-58150094 Msa0338010:five_prime_UTR 40.0%
! GTCCTTTTCCTTCAACAAAG+AGG + chr2_4:58150097-58150116 Msa0338010:five_prime_UTR 40.0%
GTTTCGATCCTCTTTGTTGA+AGG - chr2_4:58150108-58150127 None:intergenic 40.0%
AGGATCGAAACCCAAGAAAA+TGG + chr2_4:58150117-58150136 Msa0338010:exon 40.0%
GGATCGAAACCCAAGAAAAT+GGG + chr2_4:58150118-58150137 Msa0338010:exon 40.0%
AGTAATGAGAGAAAACTCGC+TGG + chr2_4:58150247-58150266 Msa0338010:CDS 40.0%
! TAACTGGTTTCGTGGCAAAT+GGG + chr2_4:58150276-58150295 Msa0338010:CDS 40.0%
GATCATAGAGAGGATACGAA+GGG - chr2_4:58150309-58150328 None:intergenic 40.0%
CAAGAAGAAGGATCATAGAG+AGG - chr2_4:58150319-58150338 None:intergenic 40.0%
TCTATGAAGGGACAAGAAGA+AGG - chr2_4:58150331-58150350 None:intergenic 40.0%
GAGTGAATTCGCCAGAATTA+AGG + chr2_4:58150503-58150522 Msa0338010:intron 40.0%
GAGTTGAGTCACCTTAATTC+TGG - chr2_4:58150517-58150536 None:intergenic 40.0%
AAGGTGACTCAACTCAAAGT+AGG + chr2_4:58150522-58150541 Msa0338010:intron 40.0%
ATTCTTGGGTGCCAAAAACA+AGG + chr2_4:58150793-58150812 Msa0338010:CDS 40.0%
!! GATAGCACTTTGCTTTGAGA+AGG - chr2_4:58150823-58150842 None:intergenic 40.0%
CAAAGAACACATCACTTGCA+TGG - chr2_4:58150975-58150994 None:intergenic 40.0%
ACAAAACATGGGTTCACACA+AGG - chr2_4:58151041-58151060 None:intergenic 40.0%
TCCATGTTGGTGTGAAACAA+TGG + chr2_4:58151140-58151159 Msa0338010:intron 40.0%
TCCATTGTTTCACACCAACA+TGG - chr2_4:58151144-58151163 None:intergenic 40.0%
! AAAGTATGAAACGGGTTTGG+TGG + chr2_4:58151175-58151194 Msa0338010:intron 40.0%
TTAAAAATGTCCCCGGCAAA+AGG - chr2_4:58151209-58151228 None:intergenic 40.0%
TGTCTACGTTCCAAAGAAGA+GGG - chr2_4:58151451-58151470 None:intergenic 40.0%
ATGTCTACGTTCCAAAGAAG+AGG - chr2_4:58151452-58151471 None:intergenic 40.0%
!! AGTAACTTTGGGGTGTAACT+TGG + chr2_4:58152305-58152324 Msa0338010:intron 40.0%
GGGGTGTAACTTGGATTATT+TGG + chr2_4:58152314-58152333 Msa0338010:intron 40.0%
! AACTTGGATTATTTGGGGTG+TGG + chr2_4:58152321-58152340 Msa0338010:intron 40.0%
! ACTTGGATTATTTGGGGTGT+GGG + chr2_4:58152322-58152341 Msa0338010:intron 40.0%
!!! GTTTTCTTTTTCGGTCTAGC+TGG - chr2_4:58152509-58152528 None:intergenic 40.0%
AACACGTGTTTCTCAGACTA+TGG + chr2_4:58152526-58152545 Msa0338010:intron 40.0%
ACGTGCAAAGAAGAAAAGGT+TGG - chr2_4:58153116-58153135 None:intergenic 40.0%
AGGAAAAGTCATGAAAGCAG+AGG - chr2_4:58153549-58153568 None:intergenic 40.0%
ATGGAGAGGAATGGATGTAT+TGG + chr2_4:58154100-58154119 Msa0338010:CDS 40.0%
! TGGTTTTCAACACATGGCAT+TGG + chr2_4:58154120-58154139 Msa0338010:CDS 40.0%
!! TTTTCAACACATGGCATTGG+TGG + chr2_4:58154123-58154142 Msa0338010:CDS 40.0%
TGGGATTATATTCAAGAGGG+TGG + chr2_4:58154261-58154280 Msa0338010:CDS 40.0%
TTATATTCAAGAGGGTGGCA+AGG + chr2_4:58154266-58154285 Msa0338010:CDS 40.0%
CGTTTCATTGCGTTCTACAA+AGG + chr2_4:58154306-58154325 Msa0338010:CDS 40.0%
! TTTGACAACTTGGGCTAGAT+GGG + chr2_4:58154329-58154348 Msa0338010:CDS 40.0%
TGTAAATCCAGCTCAAACCA+AGG + chr2_4:58154362-58154381 Msa0338010:CDS 40.0%
ACAACTTACTCGTAATGCAC+AGG - chr2_4:58154408-58154427 None:intergenic 40.0%
! TCTCTCATGCATGATTTTGC+TGG - chr2_4:58154882-58154901 None:intergenic 40.0%
GGGTACTTCAAACCAAAGAA+TGG - chr2_4:58154912-58154931 None:intergenic 40.0%
AGAAAAGATGCACATCCAGA+AGG + chr2_4:58155029-58155048 Msa0338010:CDS 40.0%
GAAAAGATGCACATCCAGAA+GGG + chr2_4:58155030-58155049 Msa0338010:CDS 40.0%
TGCCAACAGATTGTAGTCAT+TGG + chr2_4:58155066-58155085 Msa0338010:CDS 40.0%
CACCAATGACTACAATCTGT+TGG - chr2_4:58155071-58155090 None:intergenic 40.0%
GTATTTGGTACAATCAAGGC+AGG - chr2_4:58155330-58155349 None:intergenic 40.0%
! GGTAGTTGTTGCTGTTGTGT+TGG - chr2_4:58150215-58150234 None:intergenic 45.0%
AACTCGCTGGAAGATGTAAC+TGG + chr2_4:58150260-58150279 Msa0338010:CDS 45.0%
! GGAAGATGTAACTGGTTTCG+TGG + chr2_4:58150268-58150287 Msa0338010:CDS 45.0%
! GTAACTGGTTTCGTGGCAAA+TGG + chr2_4:58150275-58150294 Msa0338010:CDS 45.0%
GGATCATAGAGAGGATACGA+AGG - chr2_4:58150310-58150329 None:intergenic 45.0%
CGCCGCTAGTAACTACAAAA+TGG - chr2_4:58151083-58151102 None:intergenic 45.0%
! GTTACTAGCGGCGAATTTTG+TGG + chr2_4:58151090-58151109 Msa0338010:intron 45.0%
! GTGGTTTGTGGGTGAAAAAG+GGG - chr2_4:58151674-58151693 None:intergenic 45.0%
! GGTGGTTTGTGGGTGAAAAA+GGG - chr2_4:58151675-58151694 None:intergenic 45.0%
! TGGTGGTTTGTGGGTGAAAA+AGG - chr2_4:58151676-58151695 None:intergenic 45.0%
! TTTTCACCCACAAACCACCA+TGG + chr2_4:58151676-58151695 Msa0338010:intron 45.0%
! GCATATCCATGGTGGTTTGT+GGG - chr2_4:58151685-58151704 None:intergenic 45.0%
TGCATATCCATGGTGGTTTG+TGG - chr2_4:58151686-58151705 None:intergenic 45.0%
CGCTCCATCCGTCTTATATT+TGG + chr2_4:58152561-58152580 Msa0338010:intron 45.0%
CAAAATGGACCACATGACAC+TGG + chr2_4:58153503-58153522 Msa0338010:intron 45.0%
! CAGAGGAGTGCATCTGAATT+AGG - chr2_4:58153532-58153551 None:intergenic 45.0%
TCACTGAAAGGAGTTGAGTG+AGG + chr2_4:58153710-58153729 Msa0338010:intron 45.0%
!! CATTGGGCGTGTTCTAAAGT+TGG + chr2_4:58154055-58154074 Msa0338010:CDS 45.0%
! AAGAGTGGTAATGCATGGAG+AGG + chr2_4:58154086-58154105 Msa0338010:CDS 45.0%
! TGGTAATGCATGGAGAGGAA+TGG + chr2_4:58154091-58154110 Msa0338010:CDS 45.0%
! GTTTGACAACTTGGGCTAGA+TGG + chr2_4:58154328-58154347 Msa0338010:CDS 45.0%
! AAGAAGACCTTGGTTTGAGC+TGG - chr2_4:58154372-58154391 None:intergenic 45.0%
ACTTGCGCAAAGTATGTGTG+AGG + chr2_4:58154571-58154590 Msa0338010:intron 45.0%
! GTGGTGGCACACATAGATTT+TGG - chr2_4:58154643-58154662 None:intergenic 45.0%
GGTAAATTAGGGGGAGAGAT+TGG + chr2_4:58154850-58154869 Msa0338010:intron 45.0%
!! TTTGGTTTGAAGTACCCAGC+AGG + chr2_4:58154915-58154934 Msa0338010:CDS 45.0%
TTCACTATCACCCAAGCCAT+TGG - chr2_4:58154945-58154964 None:intergenic 45.0%
! TGTAGTCATTGGTGTCTTCC+AGG + chr2_4:58155077-58155096 Msa0338010:CDS 45.0%
TTCCAGGACTTCCTGATACT+TGG + chr2_4:58155093-58155112 Msa0338010:CDS 45.0%
TTCCAAGTATCAGGAAGTCC+TGG - chr2_4:58155098-58155117 None:intergenic 45.0%
CTTCTTCATGCAGCTCTCTT+TGG + chr2_4:58155122-58155141 Msa0338010:CDS 45.0%
!! TATTTAAAAAATACATTTAT+TGG + chr2_4:58151382-58151401 Msa0338010:intron 5.0%
!! ATTTAAAAAATACATTTATT+GGG + chr2_4:58151383-58151402 Msa0338010:intron 5.0%
!!! ATATTTAATTTATCTTTTTT+TGG + chr2_4:58151498-58151517 Msa0338010:intron 5.0%
!! GTAAAAAATTTAATAAATTT+GGG - chr2_4:58151580-58151599 None:intergenic 5.0%
!! TGTAAAAAATTTAATAAATT+TGG - chr2_4:58151581-58151600 None:intergenic 5.0%
!!! TATTAATATTATTTTCAAAA+AGG - chr2_4:58153403-58153422 None:intergenic 5.0%
!! GTATGAAACGGGTTTGGTGG+AGG + chr2_4:58151178-58151197 Msa0338010:intron 50.0%
! GTGGAGGCTTTTCCTTTTGC+CGG + chr2_4:58151194-58151213 Msa0338010:intron 50.0%
! TGGAGGCTTTTCCTTTTGCC+GGG + chr2_4:58151195-58151214 Msa0338010:intron 50.0%
TGTGGGTGAAAAAGGGGTGA+GGG - chr2_4:58151668-58151687 None:intergenic 50.0%
TTGTGGGTGAAAAAGGGGTG+AGG - chr2_4:58151669-58151688 None:intergenic 50.0%
GGGGACGTGCAAAGAAGAAA+AGG - chr2_4:58153120-58153139 None:intergenic 50.0%
AATGGACCACATGACACTGG+TGG + chr2_4:58153506-58153525 Msa0338010:intron 50.0%
ATTAGGCCACCAGTGTCATG+TGG - chr2_4:58153515-58153534 None:intergenic 50.0%
GAACACGCCCAATGTTCTCA+CGG - chr2_4:58154049-58154068 None:intergenic 50.0%
CGGTTGTGCATTTCCAGTGT+GGG - chr2_4:58154150-58154169 None:intergenic 50.0%
ACGGTTGTGCATTTCCAGTG+TGG - chr2_4:58154151-58154170 None:intergenic 50.0%
CACTGGAAATGCACAACCGT+AGG + chr2_4:58154151-58154170 Msa0338010:intron 50.0%
! AAGCCATTGGTGTTCCTGCT+GGG - chr2_4:58154932-58154951 None:intergenic 50.0%
AGCAGGAACACCAATGGCTT+GGG + chr2_4:58154932-58154951 Msa0338010:CDS 50.0%
! GGAGGCTTTTCCTTTTGCCG+GGG + chr2_4:58151196-58151215 Msa0338010:intron 55.0%
GTACCCAGCAGGAACACCAA+TGG + chr2_4:58154926-58154945 Msa0338010:CDS 55.0%
! CAAGCCATTGGTGTTCCTGC+TGG - chr2_4:58154933-58154952 None:intergenic 55.0%
CAGCAGGAACACCAATGGCT+TGG + chr2_4:58154931-58154950 Msa0338010:CDS 55.0%
!! TGGCATTGGTGGACCCACAC+TGG + chr2_4:58154134-58154153 Msa0338010:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 58150041 58155374 58150041 ID=Msa0338010;Name=Msa0338010
chr2_4 mRNA 58150041 58155374 58150041 ID=Msa0338010-mRNA-1;Parent=Msa0338010;Name=Msa0338010-mRNA-1;_AED=0.11;_eAED=0.11;_QI=95|1|1|1|0.8|0.66|6|198|365
chr2_4 exon 58150041 58150437 58150041 ID=Msa0338010-mRNA-1:exon:17798;Parent=Msa0338010-mRNA-1
chr2_4 exon 58150689 58150860 58150689 ID=Msa0338010-mRNA-1:exon:17799;Parent=Msa0338010-mRNA-1
chr2_4 exon 58153993 58154168 58153993 ID=Msa0338010-mRNA-1:exon:17800;Parent=Msa0338010-mRNA-1
chr2_4 exon 58154260 58154418 58154260 ID=Msa0338010-mRNA-1:exon:17801;Parent=Msa0338010-mRNA-1
chr2_4 exon 58154860 58155321 58154860 ID=Msa0338010-mRNA-1:exon:17802;Parent=Msa0338010-mRNA-1
chr2_4 exon 58155350 58155374 58155350 ID=Msa0338010-mRNA-1:exon:17803;Parent=Msa0338010-mRNA-1
chr2_4 five_prime_UTR 58150041 58150135 58150041 ID=Msa0338010-mRNA-1:five_prime_utr;Parent=Msa0338010-mRNA-1
chr2_4 CDS 58150136 58150437 58150136 ID=Msa0338010-mRNA-1:cds;Parent=Msa0338010-mRNA-1
chr2_4 CDS 58150689 58150860 58150689 ID=Msa0338010-mRNA-1:cds;Parent=Msa0338010-mRNA-1
chr2_4 CDS 58153993 58154168 58153993 ID=Msa0338010-mRNA-1:cds;Parent=Msa0338010-mRNA-1
chr2_4 CDS 58154260 58154418 58154260 ID=Msa0338010-mRNA-1:cds;Parent=Msa0338010-mRNA-1
chr2_4 CDS 58154860 58155148 58154860 ID=Msa0338010-mRNA-1:cds;Parent=Msa0338010-mRNA-1
chr2_4 three_prime_UTR 58155149 58155321 58155149 ID=Msa0338010-mRNA-1:three_prime_utr;Parent=Msa0338010-mRNA-1
chr2_4 three_prime_UTR 58155350 58155374 58155350 ID=Msa0338010-mRNA-1:three_prime_utr;Parent=Msa0338010-mRNA-1
Gene Sequence

>Msa0338010

ATGGGTTTTCTGTTACCGACTCTGTTTTTACTTCTTCTGTTTTCTTCACACCAAACAAAAGCTCTTGAATTTAACTCCAACACAACAGCAACAACTACCATTTCCAGTAGTAGTAATGAGAGAAAACTCGCTGGAAGATGTAACTGGTTTCGTGGCAAATGGGTCTATGACCCTTCGTATCCTCTCTATGATCCTTCTTCTTGTCCCTTCATAGATCCACAATTCAATTGTCAAAAGTATGGCAGACCTGATACTCAGTATCAGAAATATAGATGGCAACCTTTCACTTGTTCTATACCAAGGTTCAATGCATTGGATTTTCTAGCAAAGTATAGGGGAAAAAAGATTATGTTTGTTGGTGATTCTTTGAGCTTAAACCAGTTCAATTCACTAGCATGTATGATTCATTCTTGGGTGCCAAAAACAAGGTATTCCTTCTCAAAGCAAAGTGCTATCTCCACAATAACATTTCAGGATTATGGTCTCCAATTATTTTTATTCCGCACACCATATTTGGTAGACCTTGACCGTGAGAACATTGGGCGTGTTCTAAAGTTGGACTCAATAAAGAGTGGTAATGCATGGAGAGGAATGGATGTATTGGTTTTCAACACATGGCATTGGTGGACCCACACTGGAAATGCACAACCATGGGATTATATTCAAGAGGGTGGCAAGGTGTACAAAGACATGAACCGTTTCATTGCGTTCTACAAAGGTTTGACAACTTGGGCTAGATGGGTTAACATCAATGTAAATCCAGCTCAAACCAAGGTCTTCTTTTTAGGAATTTCTCCTGTGCATTACGAGGGGAGAGATTGGAATCAACCAGCAAAATCATGCATGAGAGAAACACAACCATTCTTTGGTTTGAAGTACCCAGCAGGAACACCAATGGCTTGGGTGATAGTGAATAAAGTATTGAGCAGATTAAAGAAGCCAGTGTATTTTCTAGATGTGACAACATTGTCACAGTACAGAAAAGATGCACATCCAGAAGGGTATAGTAGTGTTATGCCAACAGATTGTAGTCATTGGTGTCTTCCAGGACTTCCTGATACTTGGAATGTTCTTCTTCATGCAGCTCTCTTTGGTTGA
Protein sequence

>Msa0338010

MGFLLPTLFLLLLFSSHQTKALEFNSNTTATTTISSSSNERKLAGRCNWFRGKWVYDPSYPLYDPSSCPFIDPQFNCQKYGRPDTQYQKYRWQPFTCSIPRFNALDFLAKYRGKKIMFVGDSLSLNQFNSLACMIHSWVPKTRYSFSKQSAISTITFQDYGLQLFLFRTPYLVDLDRENIGRVLKLDSIKSGNAWRGMDVLVFNTWHWWTHTGNAQPWDYIQEGGKVYKDMNRFIAFYKGLTTWARWVNINVNPAQTKVFFLGISPVHYEGRDWNQPAKSCMRETQPFFGLKYPAGTPMAWVIVNKVLSRLKKPVYFLDVTTLSQYRKDAHPEGYSSVMPTDCSHWCLPGLPDTWNVLLHAALFG*