AlfalfaGEDB | Msa0221140

M. sativa cultivar zhongmu-4 / Msa0221140

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005911 GO:0008150 GO:0008374 GO:0009506 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0030054 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071554
PFAM: PF14416.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0221140	XP_013465226.1	97.826	368	5	1	1	365	1	368	0.0	751

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0221140	sp\|Q9SIN2\|TBL39_ARATH	66.279	344	111	3	26	364	22	365	3.02e-179	504

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0221140	A0A072VCD5	97.826	368	5	1	1	365	1	368	0.0	751

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0221140	Msa0262700	0.905043	6.512269e-80	-8.615850e-47
Msa0221140	Msa0300430	0.948324	1.183851e-106	-8.615850e-47
Msa0221140	Msa0338010	0.925718	1.255410e-90	-8.615850e-47
Msa0221140	Msa0456070	0.803284	3.750941e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0221140	MtrunA17_Chr2g0325481	97.826	368	5	1	1	365	1	368	0.0	751
Msa0221140	MtrunA17_Chr4g0041011	75.000	360	85	1	4	363	9	363	0.0	584
Msa0221140	MtrunA17_Chr2g0283581	59.563	366	137	5	1	363	1	358	2.07e-159	451
Msa0221140	MtrunA17_Chr4g0045951	55.890	365	158	2	1	364	1	363	1.23e-152	434
Msa0221140	MtrunA17_Chr4g0045941	55.191	366	147	3	1	364	1	351	8.73e-149	424
Msa0221140	MtrunA17_Chr3g0097891	54.829	321	142	2	47	364	31	351	1.63e-133	385
Msa0221140	MtrunA17_Chr2g0314801	55.799	319	137	3	46	360	32	350	2.28e-131	380
Msa0221140	MtrunA17_Chr3g0085071	52.905	327	153	1	38	363	37	363	2.29e-130	377
Msa0221140	MtrunA17_Chr3g0091031	50.448	335	165	1	30	363	14	348	2.24e-127	369
Msa0221140	MtrunA17_Chr3g0085081	52.201	318	151	1	47	363	31	348	1.05e-125	365

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0221140	AT2G42570.1	66.279	344	111	3	26	364	22	365	3.06e-180	504
Msa0221140	AT2G31110.2	62.928	321	118	1	44	363	42	362	8.63e-166	468
Msa0221140	AT1G29050.1	59.882	339	134	2	27	363	40	378	1.57e-157	447
Msa0221140	AT2G34070.1	59.692	325	129	2	41	363	59	383	2.28e-150	429
Msa0221140	AT3G14850.2	55.108	323	141	2	42	360	29	351	4.07e-131	379
Msa0221140	AT1G78710.1	50.153	327	157	4	42	364	33	357	3.04e-121	354
Msa0221140	AT2G30900.1	50.000	324	156	4	47	364	43	366	1.48e-119	350
Msa0221140	AT2G34070.2	60.643	249	96	2	117	363	1	249	1.89e-111	325
Msa0221140	AT5G58600.1	47.619	336	158	5	47	364	66	401	1.36e-110	329
Msa0221140	AT2G31110.3	61.927	218	83	0	44	261	42	259	7.96e-108	317

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Find 90 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTCGCTGGAAGATGTAAC+TGG	0.464742	2_1:-69745107	Msa0221140:CDS
AAGAAGACCTTGGTTTGAGC+TGG	0.426020	2_1:+69741831	None:intergenic
AAGAGTGGTAATGCATGGAG+AGG	0.592479	2_1:-69742114	Msa0221140:CDS
AAGCCATTGGTGTTCCTGCT+GGG	0.502968	2_1:+69741271	None:intergenic
AAGTTGGACTCAATAAAGAG+TGG	0.679146	2_1:-69742129	Msa0221140:CDS
ACAACTTACTCGTAATGCAC+AGG	0.597709	2_1:+69741795	None:intergenic
ACCATAGGAATTATTTAACT+TGG	0.452262	2_1:-69740986	Msa0221140:three_prime_UTR
ACCTTGACCGTGAGAACATT+GGG	0.399023	2_1:-69742161	Msa0221140:CDS
ACGGTTGTGCATTTCCAGTG+TGG	0.583332	2_1:+69742052	None:intergenic
ACTAACCTGAAATGTTATTG+TGG	0.576103	2_1:+69744530	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAGAAGTAAAAACAGAGT+CGG	+	chr2_1:69740953-69740972	None:intergenic	30.0%
TTGAATTGTGGATCTATGAA+GGG	+	chr2_1:69741142-69741161	None:intergenic	30.0%
ATTGAATTGTGGATCTATGA+AGG	+	chr2_1:69741143-69741162	None:intergenic	30.0%
CTCAGTATCAGAAATATAGA+TGG	-	chr2_1:69741188-69741207	Msa0221140:CDS	30.0%
AACAGAAGCTATGATAGAAA+TGG	+	chr2_1:69741256-69741275	None:intergenic	30.0%
TGTTAGATTTAGTGTAGGTT+TGG	-	chr2_1:69741272-69741291	Msa0221140:CDS	30.0%
GAATTAAAGTGACTCAAAGT+AGG	-	chr2_1:69741314-69741333	Msa0221140:CDS	30.0%
GAGTAAATCTATCAGAATCA+CGG	+	chr2_1:69741343-69741362	None:intergenic	30.0%
TAGCATGTATGATTCATTCT+TGG	-	chr2_1:69741570-69741589	Msa0221140:intron	30.0%
AGCATGTATGATTCATTCTT+GGG	-	chr2_1:69741571-69741590	Msa0221140:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	69740840	69745348	69740840	ID=Msa0221140;Name=Msa0221140
chr2_1	mRNA	69740840	69745348	69740840	ID=Msa0221140-mRNA-1;Parent=Msa0221140;Name=Msa0221140-mRNA-1;_AED=0.09;_eAED=0.09;_QI=95\|1\|1\|1\|0.8\|0.66\|6\|206\|365
chr2_1	exon	69740840	69740870	69740840	ID=Msa0221140-mRNA-1:exon:19800;Parent=Msa0221140-mRNA-1
chr2_1	exon	69740899	69741362	69740899	ID=Msa0221140-mRNA-1:exon:19799;Parent=Msa0221140-mRNA-1
chr2_1	exon	69741804	69741962	69741804	ID=Msa0221140-mRNA-1:exon:19798;Parent=Msa0221140-mRNA-1
chr2_1	exon	69742054	69742229	69742054	ID=Msa0221140-mRNA-1:exon:19797;Parent=Msa0221140-mRNA-1
chr2_1	exon	69744536	69744707	69744536	ID=Msa0221140-mRNA-1:exon:19796;Parent=Msa0221140-mRNA-1
chr2_1	exon	69744952	69745348	69744952	ID=Msa0221140-mRNA-1:exon:19795;Parent=Msa0221140-mRNA-1
chr2_1	five_prime_UTR	69745254	69745348	69745254	ID=Msa0221140-mRNA-1:five_prime_utr;Parent=Msa0221140-mRNA-1
chr2_1	CDS	69744952	69745253	69744952	ID=Msa0221140-mRNA-1:cds;Parent=Msa0221140-mRNA-1
chr2_1	CDS	69744536	69744707	69744536	ID=Msa0221140-mRNA-1:cds;Parent=Msa0221140-mRNA-1
chr2_1	CDS	69742054	69742229	69742054	ID=Msa0221140-mRNA-1:cds;Parent=Msa0221140-mRNA-1
chr2_1	CDS	69741804	69741962	69741804	ID=Msa0221140-mRNA-1:cds;Parent=Msa0221140-mRNA-1
chr2_1	CDS	69741074	69741362	69741074	ID=Msa0221140-mRNA-1:cds;Parent=Msa0221140-mRNA-1
chr2_1	three_prime_UTR	69740899	69741073	69740899	ID=Msa0221140-mRNA-1:three_prime_utr;Parent=Msa0221140-mRNA-1
chr2_1	three_prime_UTR	69740840	69740870	69740840	ID=Msa0221140-mRNA-1:three_prime_utr;Parent=Msa0221140-mRNA-1

Gene Sequence

>Msa0221140

ATGGGTTTTCTGTTACCGACTCTGTTTTTACTTCTTCTGTTTTCTTCACACCAAACAAAAGCTCTTGAATTTAACTCCAACACAACAGCAACAACTACCATTTCCAGTAGTAGTAATGAGAGAAAACTCGCTGGAAGATGTAACTGGTTTCGTGGCAAATGGGTCTATGACCCTTCGTATCCTCTCTATGATCCTTCTTCTTGTCCCTTCATAGATCCACAATTCAATTGTCAAAAGTATGGCAGACCTGATACTCAGTATCAGAAATATAGATGGCAACCTTTCACTTGTTCTATACCAAGGTTCAATGCATTGGATTTTCTAGCAAAGTACAGGGGAAAAAAGATTATGTTTGTTGGTGATTCTTTGAGCTTAAACCAGTTCAATTCACTAGCATGTATGATTCATTCTTGGGTGCCAAAAACAAGGTATTCCTTCTCAAAGCAAAGTGCTATCTCCACAATAACATTTCAGGATTATGGTCTCCAATTATTTCTATTCCGCACACCATATTTGGTAGACCTTGACCGTGAGAACATTGGGCGTGTTCTAAAGTTGGACTCAATAAAGAGTGGTAATGCATGGAGAGGAATGGATGTATTGGTCTTCAACACATGGCATTGGTGGACCCACACTGGAAATGCACAACCATGGGATTATATTCAAGAGGGTGGCAAGGTGTACAAAGACATGAACCGTTTCATTGCGTTCTACAAAGGTTTGACAACTTGGGCTAGATGGGTTAACATCAATGTAAATCCAGCTCAAACCAAGGTCTTCTTTTTAGGAATTTCTCCTGTGCATTACGAGGGGAGAGATTGGAATCAACCAGCAAAATCATGCATGAGAGAAACACAACCATTCTTTGGTTTGAAGTACCCAGCAGGAACACCAATGGCTTGGGTAATAGTGAATAAAGTATTGAGCAGATTAAAGAAGCCAGTGTATTTTCTAGATGTGACAACATTGTCACAGTACAGAAAAGATGCACATCCAGAAGGGTATAGTAGTGTTATGCCAACAGATTGTAGTCATTGGTGTCTTCCAGGACTTCCTGATACTTGGAATGTTCTTCTTCATGCAGCTCTCTTTGGTTGA

Protein sequence

>Msa0221140

MGFLLPTLFLLLLFSSHQTKALEFNSNTTATTTISSSSNERKLAGRCNWFRGKWVYDPSYPLYDPSSCPFIDPQFNCQKYGRPDTQYQKYRWQPFTCSIPRFNALDFLAKYRGKKIMFVGDSLSLNQFNSLACMIHSWVPKTRYSFSKQSAISTITFQDYGLQLFLFRTPYLVDLDRENIGRVLKLDSIKSGNAWRGMDVLVFNTWHWWTHTGNAQPWDYIQEGGKVYKDMNRFIAFYKGLTTWARWVNINVNPAQTKVFFLGISPVHYEGRDWNQPAKSCMRETQPFFGLKYPAGTPMAWVIVNKVLSRLKKPVYFLDVTTLSQYRKDAHPEGYSSVMPTDCSHWCLPGLPDTWNVLLHAALFG*