AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0221140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221140 MtrunA17_Chr2g0325481 97.826 368 5 1 1 365 1 368 0.0 751
Msa0221140 MtrunA17_Chr4g0041011 75.000 360 85 1 4 363 9 363 0.0 584
Msa0221140 MtrunA17_Chr2g0283581 59.563 366 137 5 1 363 1 358 2.07e-159 451
Msa0221140 MtrunA17_Chr4g0045951 55.890 365 158 2 1 364 1 363 1.23e-152 434
Msa0221140 MtrunA17_Chr4g0045941 55.191 366 147 3 1 364 1 351 8.73e-149 424
Msa0221140 MtrunA17_Chr3g0097891 54.829 321 142 2 47 364 31 351 1.63e-133 385
Msa0221140 MtrunA17_Chr2g0314801 55.799 319 137 3 46 360 32 350 2.28e-131 380
Msa0221140 MtrunA17_Chr3g0085071 52.905 327 153 1 38 363 37 363 2.29e-130 377
Msa0221140 MtrunA17_Chr3g0091031 50.448 335 165 1 30 363 14 348 2.24e-127 369
Msa0221140 MtrunA17_Chr3g0085081 52.201 318 151 1 47 363 31 348 1.05e-125 365
Msa0221140 MtrunA17_Chr5g0413851 51.796 334 155 4 35 364 29 360 6.61e-123 358
Msa0221140 MtrunA17_Chr3g0105301 44.800 375 190 4 7 364 3 377 4.35e-116 342
Msa0221140 MtrunA17_Chr5g0430371 44.236 373 167 5 17 364 44 400 4.12e-115 340
Msa0221140 MtrunA17_Chr2g0314791 45.289 329 158 8 47 364 29 346 3.24e-101 303
Msa0221140 MtrunA17_Chr1g0171171 42.941 340 170 8 39 363 45 375 1.07e-95 290
Msa0221140 MtrunA17_Chr1g0210771 38.040 347 194 6 31 363 43 382 1.31e-91 279
Msa0221140 MtrunA17_Chr1g0175671 38.400 375 204 6 15 364 138 510 2.79e-90 280
Msa0221140 MtrunA17_Chr7g0258851 40.233 343 180 6 46 364 247 588 1.85e-89 280
Msa0221140 MtrunA17_Chr4g0071391 37.318 343 186 6 46 363 108 446 4.09e-84 262
Msa0221140 MtrunA17_Chr5g0413271 34.637 358 217 4 23 364 121 477 1.00e-81 257
Msa0221140 MtrunA17_Chr3g0113671 39.017 346 184 9 47 365 106 451 1.48e-81 256
Msa0221140 MtrunA17_Chr4g0068111 38.441 372 198 10 23 364 84 454 2.10e-81 256
Msa0221140 MtrunA17_Chr7g0269991 39.759 332 176 8 47 359 137 463 2.73e-80 253
Msa0221140 MtrunA17_Chr1g0213281 36.827 353 193 8 39 364 167 516 1.27e-78 250
Msa0221140 MtrunA17_Chr5g0437291 38.109 349 186 10 47 365 165 513 1.28e-78 250
Msa0221140 MtrunA17_Chr8g0368461 36.667 360 186 7 43 364 174 529 2.05e-77 248
Msa0221140 MtrunA17_Chr3g0114101 34.085 399 227 10 2 364 33 431 8.08e-76 240
Msa0221140 MtrunA17_Chr5g0437601 37.069 348 194 7 42 364 78 425 3.15e-75 239
Msa0221140 MtrunA17_Chr4g0006991 34.555 382 208 10 24 363 46 427 1.20e-74 237
Msa0221140 MtrunA17_Chr8g0360521 35.613 351 196 9 40 364 64 410 4.34e-71 228
Msa0221140 MtrunA17_Chr1g0209921 48.261 230 110 7 44 268 41 266 2.19e-69 218
Msa0221140 MtrunA17_Chr2g0287901 37.059 340 188 7 47 361 97 435 7.84e-69 223
Msa0221140 MtrunA17_Chr7g0259711 36.812 345 189 8 47 364 91 433 3.28e-68 221
Msa0221140 MtrunA17_Chr4g0005481 35.632 348 198 9 36 359 97 442 3.33e-68 221
Msa0221140 MtrunA17_Chr4g0005491 32.687 361 209 9 33 364 53 408 5.48e-67 217
Msa0221140 MtrunA17_Chr7g0243301 31.969 391 230 10 4 365 28 411 1.12e-66 216
Msa0221140 MtrunA17_Chr7g0242701 35.838 346 195 9 44 363 83 427 4.79e-66 215
Msa0221140 MtrunA17_Chr3g0126021 36.202 337 191 7 47 364 129 460 3.00e-62 206
Msa0221140 MtrunA17_Chr8g0384151 30.029 343 220 6 37 364 80 417 2.61e-53 182
Msa0221140 MtrunA17_Chr1g0178081 31.778 343 154 10 47 364 55 342 3.65e-50 171
Msa0221140 MtrunA17_Chr6g0456691 28.753 393 232 11 4 363 42 419 6.03e-39 144
Msa0221140 MtrunA17_Chr5g0414051 29.592 392 221 16 9 359 39 416 5.65e-38 141
Msa0221140 MtrunA17_Chr5g0414061 30.049 406 224 19 4 359 39 434 5.68e-38 141
Msa0221140 MtrunA17_Chr1g0188191 28.792 389 230 13 12 359 195 577 6.54e-38 143
Msa0221140 MtrunA17_Chr6g0476041 27.895 380 232 11 16 359 137 510 9.92e-37 139
Msa0221140 MtrunA17_Chr6g0456681 29.040 396 228 14 3 359 23 404 1.77e-34 131
Msa0221140 MtrunA17_Chr6g0455901 30.725 345 204 12 47 359 71 412 2.67e-34 130
Msa0221140 MtrunA17_Chr8g0384421 31.389 360 206 17 35 363 123 472 2.67e-34 131
Msa0221140 MtrunA17_Chr5g0414091 30.636 346 204 11 47 359 85 427 5.23e-34 130
Msa0221140 MtrunA17_Chr8g0347321 27.225 382 226 11 20 363 72 439 5.46e-33 127
Msa0221140 MtrunA17_Chr2g0283851 28.940 349 203 13 48 359 56 396 5.88e-32 124
Msa0221140 MtrunA17_Chr5g0414041 29.480 346 208 12 47 359 87 429 8.84e-32 124
Msa0221140 MtrunA17_Chr7g0226991 28.010 382 221 16 15 358 32 397 3.15e-31 122
Msa0221140 MtrunA17_Chr4g0049421 27.666 347 211 13 47 359 48 388 4.14e-30 119
Msa0221140 MtrunA17_Chr8g0391481 26.862 376 228 13 22 359 2 368 6.22e-30 118
Msa0221140 MtrunA17_Chr3g0085101 44.737 114 62 1 231 343 1 114 3.33e-29 109
Msa0221140 MtrunA17_Chr4g0070341 29.395 347 208 15 47 363 132 471 3.99e-29 117
Msa0221140 MtrunA17_Chr6g0451321 28.788 396 218 18 14 363 80 457 2.55e-28 115
Msa0221140 MtrunA17_Chr8g0387921 27.066 351 215 13 44 359 71 415 1.16e-27 112
Msa0221140 MtrunA17_Chr3g0140371 28.080 349 205 13 47 359 18 356 3.33e-27 110
Msa0221140 MtrunA17_Chr7g0221271 36.719 128 81 0 46 173 78 205 1.49e-22 94.4
Msa0221140 MtrunA17_Chr7g0221291 44.444 90 50 0 46 135 78 167 3.65e-22 92.0
Msa0221140 MtrunA17_Chr5g0429211 38.532 109 60 2 74 176 162 269 2.01e-17 81.6
Msa0221140 MtrunA17_Chr1g0209931 44.944 89 44 2 279 365 9 94 9.02e-17 75.1
Msa0221140 MtrunA17_Chr2g0287931 32.773 119 66 3 107 211 2 120 1.90e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221140 AT2G42570.1 66.279 344 111 3 26 364 22 365 3.06e-180 504
Msa0221140 AT2G31110.2 62.928 321 118 1 44 363 42 362 8.63e-166 468
Msa0221140 AT1G29050.1 59.882 339 134 2 27 363 40 378 1.57e-157 447
Msa0221140 AT2G34070.1 59.692 325 129 2 41 363 59 383 2.28e-150 429
Msa0221140 AT3G14850.2 55.108 323 141 2 42 360 29 351 4.07e-131 379
Msa0221140 AT1G78710.1 50.153 327 157 4 42 364 33 357 3.04e-121 354
Msa0221140 AT2G30900.1 50.000 324 156 4 47 364 43 366 1.48e-119 350
Msa0221140 AT2G34070.2 60.643 249 96 2 117 363 1 249 1.89e-111 325
Msa0221140 AT5G58600.1 47.619 336 158 5 47 364 66 401 1.36e-110 329
Msa0221140 AT2G31110.3 61.927 218 83 0 44 261 42 259 7.96e-108 317
Msa0221140 AT2G30010.1 45.322 342 163 5 47 364 56 397 3.09e-105 315
Msa0221140 AT2G30010.2 45.322 342 163 5 47 364 99 440 3.22e-105 317
Msa0221140 AT2G31110.1 63.768 207 74 1 158 363 8 214 4.30e-104 305
Msa0221140 AT3G14850.1 55.242 248 107 2 117 360 1 248 2.40e-95 285
Msa0221140 AT3G54260.1 42.515 334 167 6 47 364 51 375 6.78e-92 280
Msa0221140 AT5G06700.1 42.274 343 171 7 47 364 255 595 4.45e-91 285
Msa0221140 AT3G12060.1 39.678 373 197 7 19 364 165 536 1.59e-89 280
Msa0221140 AT1G78710.2 41.896 327 131 5 42 364 26 297 1.41e-87 266
Msa0221140 AT2G30900.2 50.424 236 109 3 47 280 43 272 1.31e-84 258
Msa0221140 AT3G62390.1 38.544 371 201 9 19 364 104 472 1.83e-82 259
Msa0221140 AT5G58600.2 51.786 224 106 2 47 268 66 289 5.50e-82 252
Msa0221140 AT5G20590.1 38.571 350 184 9 41 364 136 480 7.70e-82 258
Msa0221140 AT5G49340.1 37.960 353 190 8 39 364 92 442 1.23e-80 254
Msa0221140 AT1G60790.1 38.211 369 194 10 26 364 169 533 3.88e-80 255
Msa0221140 AT3G55990.1 37.464 347 190 9 46 365 139 485 3.76e-77 246
Msa0221140 AT2G40150.1 35.977 353 193 8 46 365 70 422 8.64e-76 240
Msa0221140 AT3G11030.1 38.000 350 179 10 47 364 106 449 8.53e-75 239
Msa0221140 AT2G40160.2 35.817 349 192 9 47 364 77 424 3.44e-74 237
Msa0221140 AT2G40160.1 36.047 344 188 9 47 359 77 419 5.02e-74 236
Msa0221140 AT1G73140.1 36.544 353 194 8 38 364 58 406 4.32e-73 233
Msa0221140 AT5G19160.1 36.388 371 193 10 24 361 82 442 5.74e-73 234
Msa0221140 AT5G01360.2 35.756 344 195 7 46 363 35 378 5.80e-72 229
Msa0221140 AT5G01360.1 35.756 344 195 7 46 363 91 434 2.01e-71 229
Msa0221140 AT5G01620.3 34.402 343 198 8 42 359 113 453 1.04e-70 228
Msa0221140 AT5G01620.2 34.402 343 198 8 42 359 105 445 1.10e-70 228
Msa0221140 AT5G01620.1 34.402 343 198 8 42 359 105 445 1.10e-70 228
Msa0221140 AT2G40320.1 35.632 348 199 8 42 364 76 423 8.05e-70 225
Msa0221140 AT3G06080.2 36.550 342 189 7 47 361 103 443 1.23e-69 226
Msa0221140 AT5G06230.2 35.574 357 201 7 36 364 10 365 4.60e-68 219
Msa0221140 AT5G06230.1 35.574 357 201 7 36 364 51 406 1.11e-67 219
Msa0221140 AT3G11570.1 37.538 325 180 7 63 365 98 421 5.75e-67 218
Msa0221140 AT2G38320.1 33.333 351 204 9 44 364 55 405 3.83e-64 210
Msa0221140 AT1G48880.1 33.973 365 194 8 21 364 102 440 4.72e-62 205
Msa0221140 AT3G62390.2 38.258 264 145 6 26 271 111 374 3.07e-57 192
Msa0221140 AT5G20590.2 39.431 246 128 7 41 269 136 377 1.48e-55 186
Msa0221140 AT5G19160.2 36.923 260 144 4 24 269 82 335 9.10e-54 181
Msa0221140 AT2G38320.2 31.056 322 194 7 71 364 1 322 4.16e-53 179
Msa0221140 AT2G40160.3 35.470 234 139 4 47 268 77 310 3.17e-50 171
Msa0221140 AT3G06080.1 37.131 237 135 3 47 269 103 339 4.27e-49 169
Msa0221140 AT5G06230.3 35.827 254 143 4 36 269 51 304 4.40e-46 160
Msa0221140 AT5G51640.1 28.571 378 222 11 23 359 114 484 5.19e-40 148
Msa0221140 AT1G70230.1 29.513 349 198 11 47 363 79 411 6.06e-40 146
Msa0221140 AT2G14530.1 29.049 389 240 12 7 363 22 406 7.60e-40 146
Msa0221140 AT4G25360.1 27.179 390 225 11 16 359 144 520 7.51e-37 140
Msa0221140 AT4G25360.2 27.179 390 225 11 16 359 144 520 7.51e-37 140
Msa0221140 AT5G64020.1 29.494 356 208 12 47 363 57 408 5.21e-35 133
Msa0221140 AT5G64470.3 26.744 344 223 10 47 364 55 395 1.47e-34 131
Msa0221140 AT1G01430.1 29.301 372 215 12 32 365 88 449 1.34e-33 130
Msa0221140 AT5G64470.2 26.857 350 221 11 47 364 55 401 4.15e-33 127
Msa0221140 AT5G15890.1 28.065 367 219 12 25 359 165 518 1.47e-32 128
Msa0221140 AT5G15900.1 26.934 349 217 11 47 363 67 409 3.66e-32 125
Msa0221140 AT5G15900.2 26.934 349 217 11 47 363 86 428 4.97e-32 125
Msa0221140 AT2G37720.1 30.939 362 183 14 47 363 143 482 5.01e-32 125
Msa0221140 AT5G20680.6 28.226 372 220 14 23 363 102 457 2.15e-31 124
Msa0221140 AT3G28150.1 30.966 352 199 16 46 363 69 410 3.10e-31 122
Msa0221140 AT3G02440.2 26.513 347 212 12 47 359 135 472 4.95e-31 123
Msa0221140 AT3G02440.1 26.513 347 212 12 47 359 127 464 5.19e-31 123
Msa0221140 AT5G20680.2 28.226 372 220 14 23 363 178 533 5.27e-31 123
Msa0221140 AT5G20680.4 29.023 348 208 13 47 363 212 551 6.62e-31 123
Msa0221140 AT5G20680.1 29.023 348 208 13 47 363 212 551 6.62e-31 123
Msa0221140 AT5G20680.3 29.023 348 208 13 47 363 212 551 6.62e-31 123
Msa0221140 AT5G20680.5 29.023 348 208 13 47 363 212 551 6.62e-31 123
Msa0221140 AT4G11090.1 25.679 405 243 15 8 365 35 428 8.34e-29 116
Msa0221140 AT4G01080.1 27.901 362 214 12 33 359 77 426 2.82e-28 115
Msa0221140 AT4G23790.1 25.275 364 217 13 46 365 78 430 5.56e-23 99.8
Msa0221140 AT5G64470.4 26.378 254 166 8 47 282 55 305 5.25e-21 92.8
Msa0221140 AT5G64470.1 26.378 254 166 8 47 282 55 305 7.35e-21 92.4

Find 90 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCATGCAGCTCTCTT+TGG 0.210145 2_1:-69741078 Msa0221140:CDS
TCTATTCCGCACACCATATT+TGG 0.219542 2_1:-69742187 Msa0221140:CDS
TTGTAATTGGATAATAAAAT+TGG 0.234987 2_1:-69740948 Msa0221140:three_prime_UTR
TATCTCCACAATAACATTTC+AGG 0.240854 2_1:-69744535 Msa0221140:intron
ATTGTACCAAATACACTCTT+TGG 0.242280 2_1:-69740862 Msa0221140:intron
AGGATCGAAACCCAAGAAAA+TGG 0.260094 2_1:-69745250 Msa0221140:exon
AGAGAAACACAACCATTCTT+TGG 0.302957 2_1:-69741303 Msa0221140:CDS
CGGTCAAGGTCTACCAAATA+TGG 0.307611 2_1:+69742174 None:intergenic
GTTTGACAACTTGGGCTAGA+TGG 0.309964 2_1:-69741872 Msa0221140:CDS
TAGCATGTATGATTCATTCT+TGG 0.316438 2_1:-69744596 Msa0221140:CDS
TGTAGTCATTGGTGTCTTCC+AGG 0.323483 2_1:-69741123 Msa0221140:CDS
GTGTGCGGAATAGAAATAAT+TGG 0.347634 2_1:+69742196 None:intergenic
CAAGCCATTGGTGTTCCTGC+TGG 0.351195 2_1:+69741270 None:intergenic
AGAAGAACATTCCAAGTATC+AGG 0.357411 2_1:+69741096 None:intergenic
TCTACAAAGGTTTGACAACT+TGG 0.371927 2_1:-69741881 Msa0221140:CDS
TTCCAGGACTTCCTGATACT+TGG 0.372897 2_1:-69741107 Msa0221140:CDS
CTTTGGTTGAAGTAGTGAAT+TGG 0.375458 2_1:-69741061 Msa0221140:exon
CAATTCAATTGTCAAAAGTA+TGG 0.391925 2_1:-69745012 Msa0221140:CDS
TATTTCTGATACTGAGTATC+AGG 0.397938 2_1:+69744985 None:intergenic
ACCTTGACCGTGAGAACATT+GGG 0.399023 2_1:-69742161 Msa0221140:CDS
AGCATGTATGATTCATTCTT+GGG 0.407416 2_1:-69744595 Msa0221140:CDS
CTCATTACTACTACTGGAAA+TGG 0.409028 2_1:+69745134 None:intergenic
ATCCTCTTTGTTGAAGGAAA+AGG 0.418304 2_1:+69745268 None:intergenic
AAGAAGACCTTGGTTTGAGC+TGG 0.426020 2_1:+69741831 None:intergenic
GTAACTGGTTTCGTGGCAAA+TGG 0.428187 2_1:-69745092 Msa0221140:CDS
GTTTCGATCCTCTTTGTTGA+AGG 0.444404 2_1:+69745262 None:intergenic
CTCAGTATCAGAAATATAGA+TGG 0.444563 2_1:-69744978 Msa0221140:CDS
TGCCAACAGATTGTAGTCAT+TGG 0.444942 2_1:-69741134 Msa0221140:CDS
TTCCTATGGTAGAAAACAAT+GGG 0.449400 2_1:+69740999 None:intergenic
ACCATAGGAATTATTTAACT+TGG 0.452262 2_1:-69740986 Msa0221140:three_prime_UTR
AACTCGCTGGAAGATGTAAC+TGG 0.464742 2_1:-69745107 Msa0221140:CDS
AGCAGGAACACCAATGGCTT+GGG 0.468530 2_1:-69741268 Msa0221140:CDS
TACATGCTAGTGAATTGAAC+TGG 0.471439 2_1:+69744610 None:intergenic
TTCACTATTACCCAAGCCAT+TGG 0.475251 2_1:+69741258 None:intergenic
TTTGACAACTTGGGCTAGAT+GGG 0.479154 2_1:-69741871 Msa0221140:CDS
ATAACACTACTATACCCTTC+TGG 0.481855 2_1:+69741156 None:intergenic
CATTGGGCGTGTTCTAAAGT+TGG 0.484902 2_1:-69742145 Msa0221140:CDS
ATTGAATTGTGGATCTATGA+AGG 0.486450 2_1:+69745026 None:intergenic
TTATGTTTCTTAATTAGTCC+AGG 0.487004 2_1:-69741026 Msa0221140:three_prime_UTR
TTCCAAGTATCAGGAAGTCC+TGG 0.489986 2_1:+69741105 None:intergenic
GGATCGAAACCCAAGAAAAT+GGG 0.490190 2_1:-69745249 Msa0221140:exon
TCTTCAACACATGGCATTGG+TGG 0.498115 2_1:-69742077 Msa0221140:CDS
GCCAAGTTAAATAATTCCTA+TGG 0.499275 2_1:+69740985 None:intergenic
GGTAGTTGTTGCTGTTGTGT+TGG 0.501527 2_1:+69745155 None:intergenic
AAGCCATTGGTGTTCCTGCT+GGG 0.502968 2_1:+69741271 None:intergenic
CAGCAGGAACACCAATGGCT+TGG 0.504711 2_1:-69741269 Msa0221140:CDS
CGGTTGTGCATTTCCAGTGT+GGG 0.509875 2_1:+69742053 None:intergenic
CTTTGTAGAACGCAATGAAA+CGG 0.518428 2_1:+69741895 None:intergenic
TAACTGGTTTCGTGGCAAAT+GGG 0.526407 2_1:-69745091 Msa0221140:CDS
CGTTTCATTGCGTTCTACAA+AGG 0.529142 2_1:-69741894 Msa0221140:CDS
GATAGCACTTTGCTTTGAGA+AGG 0.530854 2_1:+69744554 None:intergenic
CTACAAAGGTTTGACAACTT+GGG 0.543154 2_1:-69741880 Msa0221140:CDS
TGGCATTGGTGGACCCACAC+TGG 0.546946 2_1:-69742066 Msa0221140:CDS
GACCTTGACCGTGAGAACAT+TGG 0.550954 2_1:-69742162 Msa0221140:CDS
TCTATGAAGGGACAAGAAGA+AGG 0.551169 2_1:+69745039 None:intergenic
ATTCCTATGGTAGAAAACAA+TGG 0.552378 2_1:+69740998 None:intergenic
GGAAGATGTAACTGGTTTCG+TGG 0.558355 2_1:-69745099 Msa0221140:CDS
GAAAAGATGCACATCCAGAA+GGG 0.559145 2_1:-69741170 Msa0221140:CDS
TTTGGTTTGAAGTACCCAGC+AGG 0.566621 2_1:-69741285 Msa0221140:CDS
GGATCATAGAGAGGATACGA+AGG 0.570131 2_1:+69745060 None:intergenic
ATGGAGAGGAATGGATGTAT+TGG 0.570607 2_1:-69742100 Msa0221140:CDS
GGGTACTTCAAACCAAAGAA+TGG 0.571214 2_1:+69741291 None:intergenic
TGAAGTAGTGAATTGGAAAG+TGG 0.573720 2_1:-69741054 Msa0221140:three_prime_UTR
CTTTCACTTGTTCTATACCA+AGG 0.575010 2_1:-69744951 Msa0221140:intron
ACTAACCTGAAATGTTATTG+TGG 0.576103 2_1:+69744530 None:intergenic
TGGTCTTCAACACATGGCAT+TGG 0.578137 2_1:-69742080 Msa0221140:CDS
CAATAAAGAGTGGTAATGCA+TGG 0.581612 2_1:-69742119 Msa0221140:CDS
ACGGTTGTGCATTTCCAGTG+TGG 0.583332 2_1:+69742052 None:intergenic
AGAAAAGATGCACATCCAGA+AGG 0.583703 2_1:-69741171 Msa0221140:CDS
TGGTAATGCATGGAGAGGAA+TGG 0.587257 2_1:-69742109 Msa0221140:CDS
CACCAATGACTACAATCTGT+TGG 0.588126 2_1:+69741132 None:intergenic
GCCCAATGTTCTCACGGTCA+AGG 0.590015 2_1:+69742160 None:intergenic
AAGAGTGGTAATGCATGGAG+AGG 0.592479 2_1:-69742114 Msa0221140:CDS
CAGATGGGATTATATTCAAG+AGG 0.594517 2_1:-69741943 Msa0221140:CDS
ACAACTTACTCGTAATGCAC+AGG 0.597709 2_1:+69741795 None:intergenic
AGTAATGAGAGAAAACTCGC+TGG 0.602900 2_1:-69745120 Msa0221140:CDS
TTGAATTGTGGATCTATGAA+GGG 0.607685 2_1:+69745027 None:intergenic
TGTAAATCCAGCTCAAACCA+AGG 0.609530 2_1:-69741838 Msa0221140:CDS
CTTGGTATAGAACAAGTGAA+AGG 0.623660 2_1:+69744952 None:intergenic
GAACACGCCCAATGTTCTCA+CGG 0.633134 2_1:+69742154 None:intergenic
TTATATTCAAGAGGGTGGCA+AGG 0.635985 2_1:-69741934 Msa0221140:CDS
TGGGATTATATTCAAGAGGG+TGG 0.638991 2_1:-69741939 Msa0221140:CDS
CAAGAAGAAGGATCATAGAG+AGG 0.640153 2_1:+69745051 None:intergenic
ATGTATTGGTCTTCAACACA+TGG 0.655291 2_1:-69742086 Msa0221140:CDS
GTACCCAGCAGGAACACCAA+TGG 0.661011 2_1:-69741274 Msa0221140:CDS
AGATGGGATTATATTCAAGA+GGG 0.662305 2_1:-69741942 Msa0221140:CDS
GGTCTACCAAATATGGTGTG+CGG 0.672109 2_1:+69742181 None:intergenic
GATCATAGAGAGGATACGAA+GGG 0.672935 2_1:+69745061 None:intergenic
GTCACATCTAGAAAATACAC+TGG 0.675733 2_1:+69741210 None:intergenic
AAGTTGGACTCAATAAAGAG+TGG 0.679146 2_1:-69742129 Msa0221140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAAAATACATTTATTG+GGG - chr2_1:69742174-69742193 Msa0221140:CDS 10.0%
!! TTATAATTAATAAGAATGTT+TGG + chr2_1:69742649-69742668 None:intergenic 10.0%
!!! CATGAATATAATAATTTTAT+TGG + chr2_1:69742909-69742928 None:intergenic 10.0%
!! ACTATATATTTATTCATTAT+AGG - chr2_1:69743331-69743350 Msa0221140:intron 10.0%
!!! TTATTATTTGAATTATTTTC+AGG - chr2_1:69743937-69743956 Msa0221140:intron 10.0%
!! TTTATCAACTATATTTATAA+TGG - chr2_1:69744781-69744800 Msa0221140:intron 10.0%
!!! TGTAAATTTTCATATTATTT+TGG - chr2_1:69745266-69745285 Msa0221140:five_prime_UTR 10.0%
!! ACAACAAAAACATTATAAAA+AGG - chr2_1:69740842-69740861 Msa0221140:three_prime_UTR 15.0%
!!! TTTGATACTTTTGAATTTAA+TGG - chr2_1:69741698-69741717 Msa0221140:intron 15.0%
!!! ATTTTTATTATTTTCCATGT+TGG - chr2_1:69741917-69741936 Msa0221140:CDS 15.0%
!! TTAAAAAATACATTTATTGG+GGG - chr2_1:69742175-69742194 Msa0221140:CDS 15.0%
!!! TTCAATACAAATTTCATTTT+TGG + chr2_1:69742580-69742599 None:intergenic 15.0%
!! GTAAATAAATTATATGTTAG+TGG - chr2_1:69742701-69742720 Msa0221140:intron 15.0%
!! AGAATAATACAATATTAGTA+TGG - chr2_1:69742727-69742746 Msa0221140:intron 15.0%
!! ACAAAAAATAAAATTTGAAG+TGG - chr2_1:69743270-69743289 Msa0221140:intron 15.0%
!!! CAAAAAATAAAATTTGAAGT+GGG - chr2_1:69743271-69743290 Msa0221140:intron 15.0%
!! AATATAAATTACTAGAAGAA+AGG + chr2_1:69743609-69743628 None:intergenic 15.0%
!! ATGTACAATTATTATATGTA+AGG - chr2_1:69743763-69743782 Msa0221140:intron 15.0%
!!! AACTTTTATAAATCATTTGA+CGG + chr2_1:69743816-69743835 None:intergenic 15.0%
!!! AATGTTTTTTTACAGAATTA+CGG - chr2_1:69744442-69744461 Msa0221140:intron 15.0%
!!! TTTTAAATTTTAGTAGAATC+AGG - chr2_1:69744478-69744497 Msa0221140:intron 15.0%
!!! TTTAAATTTTAGTAGAATCA+GGG - chr2_1:69744479-69744498 Msa0221140:intron 15.0%
!!! TTTTGAGTTATTATTGTAAT+TGG - chr2_1:69745205-69745224 Msa0221140:CDS 15.0%
!! TTGTAATTGGATAATAAAAT+TGG - chr2_1:69745218-69745237 Msa0221140:CDS 15.0%
!! TTAGAAAATGAAAATGTGTT+TGG - chr2_1:69741447-69741466 Msa0221140:intron 20.0%
!!! AATGTGTTTGGTATTTTTTT+AGG - chr2_1:69741459-69741478 Msa0221140:intron 20.0%
!!! AAATTATGTTGGTTATGTTT+TGG - chr2_1:69741726-69741745 Msa0221140:intron 20.0%
!! TTTGAAGAAAAACAAAACAT+GGG + chr2_1:69741842-69741861 None:intergenic 20.0%
!! ATTTGAAGAAAAACAAAACA+TGG + chr2_1:69741843-69741862 None:intergenic 20.0%
!! TTATGAAAAAACTATTACCA+TGG + chr2_1:69742071-69742090 None:intergenic 20.0%
!!! TTTTTTCATAAAAAATGCCA+TGG - chr2_1:69742080-69742099 Msa0221140:CDS 20.0%
!! ATTACTATCTATTATTACCA+TGG + chr2_1:69742100-69742119 None:intergenic 20.0%
!! TAAAAAATACATTTATTGGG+GGG - chr2_1:69742176-69742195 Msa0221140:CDS 20.0%
!! TAAAAAAAGATACAAACTAG+TGG - chr2_1:69742750-69742769 Msa0221140:intron 20.0%
!! TCCTAAAGTTTCTATATAAA+GGG + chr2_1:69742954-69742973 None:intergenic 20.0%
!! TTCCTAAAGTTTCTATATAA+AGG + chr2_1:69742955-69742974 None:intergenic 20.0%
!!! ATTAGCAATAATTAGTGTTT+TGG - chr2_1:69742986-69743005 Msa0221140:intron 20.0%
!! TGATCATTACAGTTTAAAAA+GGG + chr2_1:69743106-69743125 None:intergenic 20.0%
!! TTGATCATTACAGTTTAAAA+AGG + chr2_1:69743107-69743126 None:intergenic 20.0%
!! GCTATTCACATTAAAAAAAT+AGG - chr2_1:69743216-69743235 Msa0221140:intron 20.0%
!! GTACTACAATTTAATTCATA+TGG + chr2_1:69743297-69743316 None:intergenic 20.0%
!! TTCTAGTAATTTATATTCCA+TGG - chr2_1:69743612-69743631 Msa0221140:intron 20.0%
!!! TTGAATTATTTTCAGGATTA+TGG - chr2_1:69743944-69743963 Msa0221140:intron 20.0%
!! CATAATCAATTCTGTTATTA+AGG - chr2_1:69744161-69744180 Msa0221140:intron 20.0%
!! AAAGAAATCATTAGAATGTT+TGG - chr2_1:69744567-69744586 Msa0221140:CDS 20.0%
!!! TAAATTTTACAAAATTGTGG+TGG + chr2_1:69744625-69744644 None:intergenic 20.0%
!!! CTCTAAATTTTACAAAATTG+TGG + chr2_1:69744628-69744647 None:intergenic 20.0%
!! ATAAAACTTAGTCTAGTAAT+TGG + chr2_1:69744766-69744785 None:intergenic 20.0%
!! TTATAATGGTGTATGATATT+TGG - chr2_1:69744795-69744814 Msa0221140:intron 20.0%
!! TGTATGATATTTGGTAAATT+AGG - chr2_1:69744804-69744823 Msa0221140:intron 20.0%
!! GTATGATATTTGGTAAATTA+GGG - chr2_1:69744805-69744824 Msa0221140:intron 20.0%
!! TATGATATTTGGTAAATTAG+GGG - chr2_1:69744806-69744825 Msa0221140:intron 20.0%
!! TAAATTCAAGAGCTTTTGTT+TGG + chr2_1:69740988-69741007 None:intergenic 25.0%
!! TACTTTTGACAATTGAATTG+TGG + chr2_1:69741154-69741173 None:intergenic 25.0%
! CAATTCAATTGTCAAAAGTA+TGG - chr2_1:69741154-69741173 Msa0221140:CDS 25.0%
! TAAGTAGCTAGTATAATGTT+TGG + chr2_1:69741371-69741390 None:intergenic 25.0%
!!! TTTTTTTAGGTTCAATGCAT+TGG - chr2_1:69741472-69741491 Msa0221140:intron 25.0%
! GGAAAAAAGATTATGTTTGT+TGG - chr2_1:69741515-69741534 Msa0221140:intron 25.0%
!! TAATGGTAGTGAAATTATGT+TGG - chr2_1:69741715-69741734 Msa0221140:intron 25.0%
! ACTCAATAAAGTTTCCAATT+AGG + chr2_1:69741803-69741822 None:intergenic 25.0%
!! TTATTCTTTAAAAATGTCCC+CGG + chr2_1:69742006-69742025 None:intergenic 25.0%
! TAATAAATGTGAATCTTCCA+TGG - chr2_1:69742023-69742042 Msa0221140:intron 25.0%
! GTAAATGATAGTAATAACCA+TGG + chr2_1:69742043-69742062 None:intergenic 25.0%
!! AACATATTTTCCTCTTACAA+AGG - chr2_1:69742134-69742153 Msa0221140:CDS 25.0%
!! GTTTTTAAATCCTTTGTAAG+AGG + chr2_1:69742147-69742166 None:intergenic 25.0%
!!! GTTTTTTTTTGTTGTGAAAC+CGG + chr2_1:69742836-69742855 None:intergenic 25.0%
!!! AGAAAAAGGCACTATATATT+AGG + chr2_1:69742866-69742885 None:intergenic 25.0%
! AGTTTCTATATAAAGGGAAA+AGG + chr2_1:69742948-69742967 None:intergenic 25.0%
! TCCCTTTATATAGAAACTTT+AGG - chr2_1:69742950-69742969 Msa0221140:intron 25.0%
! TTACTACTAATGATGATTGT+TGG + chr2_1:69743032-69743051 None:intergenic 25.0%
! GATCATTACAGTTTAAAAAG+GGG + chr2_1:69743105-69743124 None:intergenic 25.0%
! TTGATTGATGCTTATATCAA+GGG + chr2_1:69743196-69743215 None:intergenic 25.0%
!! TTTTTGTTCTATTGAGAACA+AGG + chr2_1:69743257-69743276 None:intergenic 25.0%
!!! TTTGAAGGGATTATCTTTTT+TGG + chr2_1:69743453-69743472 None:intergenic 25.0%
! AAAAGATAATCCCTTCAAAA+TGG - chr2_1:69743454-69743473 Msa0221140:intron 25.0%
!!! ACAAAAGTGTCACAAAATAT+TGG - chr2_1:69743553-69743572 Msa0221140:intron 25.0%
!!! CAAAAGTGTCACAAAATATT+GGG - chr2_1:69743554-69743573 Msa0221140:intron 25.0%
!! TTCATTTTCATTGAAACTAG+AGG - chr2_1:69743640-69743659 Msa0221140:intron 25.0%
! AACAGAATTGATTATGATCA+TGG + chr2_1:69744157-69744176 None:intergenic 25.0%
! AATCAATTCTGTTATTAAGG+AGG - chr2_1:69744164-69744183 Msa0221140:intron 25.0%
!! TTAGTGAATGTTTTGTACTA+TGG + chr2_1:69744670-69744689 None:intergenic 25.0%
! ATGATATTTGGTAAATTAGG+GGG - chr2_1:69744807-69744826 Msa0221140:intron 25.0%
! TTATGTTTCTTAATTAGTCC+AGG - chr2_1:69745140-69745159 Msa0221140:CDS 25.0%
! ACCATAGGAATTATTTAACT+TGG - chr2_1:69745180-69745199 Msa0221140:CDS 25.0%
!! AATATTCCAAAGAGTGTATT+TGG + chr2_1:69745313-69745332 None:intergenic 25.0%
! AACAGAAAACCCATTTTCTT+GGG + chr2_1:69740929-69740948 None:intergenic 30.0%
! TAACAGAAAACCCATTTTCT+TGG + chr2_1:69740930-69740949 None:intergenic 30.0%
AGAAGAAGTAAAAACAGAGT+CGG + chr2_1:69740953-69740972 None:intergenic 30.0%
! TTTTCTCTCATTACTACTAC+TGG + chr2_1:69741041-69741060 None:intergenic 30.0%
TTGAATTGTGGATCTATGAA+GGG + chr2_1:69741142-69741161 None:intergenic 30.0%
ATTGAATTGTGGATCTATGA+AGG + chr2_1:69741143-69741162 None:intergenic 30.0%
! TATTTCTGATACTGAGTATC+AGG + chr2_1:69741184-69741203 None:intergenic 30.0%
CTCAGTATCAGAAATATAGA+TGG - chr2_1:69741188-69741207 Msa0221140:CDS 30.0%
AACAGAAGCTATGATAGAAA+TGG + chr2_1:69741256-69741275 None:intergenic 30.0%
TGTTAGATTTAGTGTAGGTT+TGG - chr2_1:69741272-69741291 Msa0221140:CDS 30.0%
! ACTTTGAGTCACTTTAATTC+TGG + chr2_1:69741314-69741333 None:intergenic 30.0%
GAATTAAAGTGACTCAAAGT+AGG - chr2_1:69741314-69741333 Msa0221140:CDS 30.0%
GAGTAAATCTATCAGAATCA+CGG + chr2_1:69741343-69741362 None:intergenic 30.0%
TAGCATGTATGATTCATTCT+TGG - chr2_1:69741570-69741589 Msa0221140:intron 30.0%
AGCATGTATGATTCATTCTT+GGG - chr2_1:69741571-69741590 Msa0221140:intron 30.0%
TATCTCCACAATAACATTTC+AGG - chr2_1:69741631-69741650 Msa0221140:intron 30.0%
ACTAACCTGAAATGTTATTG+TGG + chr2_1:69741639-69741658 None:intergenic 30.0%
! ATCCATTTTGTAGTTACTAG+CGG - chr2_1:69741868-69741887 Msa0221140:CDS 30.0%
GTGAGCAATAAAGTATGAAA+CGG - chr2_1:69741956-69741975 Msa0221140:intron 30.0%
TGAGCAATAAAGTATGAAAC+GGG - chr2_1:69741957-69741976 Msa0221140:intron 30.0%
! AATAAAGTATGAAACGGGTT+TGG - chr2_1:69741962-69741981 Msa0221140:intron 30.0%
AAAAACTATTACCATGGCAA+TGG + chr2_1:69742065-69742084 None:intergenic 30.0%
! TTTCTTTTCCTGATCAAAAC+TGG - chr2_1:69742264-69742283 Msa0221140:intron 30.0%
!! TAAATATGCCAGTTTTGATC+AGG + chr2_1:69742275-69742294 None:intergenic 30.0%
! GAAACACGTGTTTTCTTTTT+CGG + chr2_1:69742481-69742500 None:intergenic 30.0%
AGAGAACACCAAATATAAGA+CGG + chr2_1:69742535-69742554 None:intergenic 30.0%
TCCTATGCAAAGAAAGAAAA+AGG + chr2_1:69742880-69742899 None:intergenic 30.0%
CTTGATTGATGCTTATATCA+AGG + chr2_1:69743197-69743216 None:intergenic 30.0%
!! GTAGTTTACTGACTTTATGT+AGG + chr2_1:69743535-69743554 None:intergenic 30.0%
!! AATGAAAATGAAAAGTGCCA+TGG + chr2_1:69743632-69743651 None:intergenic 30.0%
AGAAAATAGACATCACTGAA+AGG - chr2_1:69743664-69743683 Msa0221140:intron 30.0%
GGTGTTAAATATAGATCCTA+CGG + chr2_1:69744136-69744155 None:intergenic 30.0%
! TTTTGACATTTGAATGCAGA+TGG - chr2_1:69744207-69744226 Msa0221140:intron 30.0%
TTTGACATTTGAATGCAGAT+GGG - chr2_1:69744208-69744227 Msa0221140:intron 30.0%
AGATGGGATTATATTCAAGA+GGG - chr2_1:69744224-69744243 Msa0221140:intron 30.0%
AATTCCTAAAAAGAAGACCT+TGG + chr2_1:69744348-69744367 None:intergenic 30.0%
TTCCTATGGTAGAAAACAAT+GGG + chr2_1:69745170-69745189 None:intergenic 30.0%
ATTCCTATGGTAGAAAACAA+TGG + chr2_1:69745171-69745190 None:intergenic 30.0%
GCCAAGTTAAATAATTCCTA+TGG + chr2_1:69745184-69745203 None:intergenic 30.0%
ATTGTACCAAATACACTCTT+TGG - chr2_1:69745304-69745323 Msa0221140:five_prime_UTR 30.0%
ATCCTCTTTGTTGAAGGAAA+AGG + chr2_1:69740901-69740920 None:intergenic 35.0%
CTCATTACTACTACTGGAAA+TGG + chr2_1:69741035-69741054 None:intergenic 35.0%
CTTGGTATAGAACAAGTGAA+AGG + chr2_1:69741217-69741236 None:intergenic 35.0%
CTTTCACTTGTTCTATACCA+AGG - chr2_1:69741215-69741234 Msa0221140:CDS 35.0%
AGCTATGATAGAAATGGAGA+AGG + chr2_1:69741250-69741269 None:intergenic 35.0%
GCTTCTGTTAGATTTAGTGT+AGG - chr2_1:69741267-69741286 Msa0221140:CDS 35.0%
!! TGGATTTTCTAGCAAAGTAC+AGG - chr2_1:69741492-69741511 Msa0221140:intron 35.0%
!! GGATTTTCTAGCAAAGTACA+GGG - chr2_1:69741493-69741512 Msa0221140:intron 35.0%
!! GATTTTCTAGCAAAGTACAG+GGG - chr2_1:69741494-69741513 Msa0221140:intron 35.0%
TACATGCTAGTGAATTGAAC+TGG + chr2_1:69741559-69741578 None:intergenic 35.0%
!!! GAGAAGGAATACCTTGTTTT+TGG + chr2_1:69741599-69741618 None:intergenic 35.0%
CAATTAGGTGAAAGACAGAA+AGG + chr2_1:69741788-69741807 None:intergenic 35.0%
CTTTCTGTCTTTCACCTAAT+TGG - chr2_1:69741786-69741805 Msa0221140:intron 35.0%
ACTATCATTTACCATTGCCA+TGG - chr2_1:69742051-69742070 Msa0221140:intron 35.0%
ATTGTACTTTCCCTCTTCTT+TGG - chr2_1:69742228-69742247 Msa0221140:intron 35.0%
AACACCAAATATAAGACGGA+TGG + chr2_1:69742531-69742550 None:intergenic 35.0%
GAATAGAGCTTAATCGCTTA+AGG + chr2_1:69742814-69742833 None:intergenic 35.0%
TAAGCGATTAAGCTCTATTC+CGG - chr2_1:69742814-69742833 Msa0221140:intron 35.0%
! GCCTTTTTCTTTCTTTGCAT+AGG - chr2_1:69742876-69742895 Msa0221140:intron 35.0%
!! GGGAAAAGGTATTGACAATT+TGG + chr2_1:69742934-69742953 None:intergenic 35.0%
! GTTATGTGGTCCATTTTGAA+GGG + chr2_1:69743467-69743486 None:intergenic 35.0%
! TGTTATGTGGTCCATTTTGA+AGG + chr2_1:69743468-69743487 None:intergenic 35.0%
! CCTTCTTTTATCACACAATG+TGG + chr2_1:69743580-69743599 None:intergenic 35.0%
CCACATTGTGTGATAAAAGA+AGG - chr2_1:69743577-69743596 Msa0221140:intron 35.0%
TCTCAAGTAGTGTAGTACTT+TGG - chr2_1:69743704-69743723 Msa0221140:intron 35.0%
CAAATCACAATCAATCGTGT+TGG + chr2_1:69743869-69743888 None:intergenic 35.0%
ACACGATTGATTGTGATTTG+TGG - chr2_1:69743869-69743888 Msa0221140:intron 35.0%
ATACACTACATTGTTGACAC+TGG + chr2_1:69743899-69743918 None:intergenic 35.0%
GTGTGCGGAATAGAAATAAT+TGG + chr2_1:69743973-69743992 None:intergenic 35.0%
AAGTTGGACTCAATAAAGAG+TGG - chr2_1:69744037-69744056 Msa0221140:intron 35.0%
! CAATAAAGAGTGGTAATGCA+TGG - chr2_1:69744047-69744066 Msa0221140:intron 35.0%
!! ATGTATTGGTCTTCAACACA+TGG - chr2_1:69744080-69744099 Msa0221140:intron 35.0%
CAGATGGGATTATATTCAAG+AGG - chr2_1:69744223-69744242 Msa0221140:intron 35.0%
CTTTGTAGAACGCAATGAAA+CGG + chr2_1:69744274-69744293 None:intergenic 35.0%
TCTACAAAGGTTTGACAACT+TGG - chr2_1:69744285-69744304 Msa0221140:intron 35.0%
CTACAAAGGTTTGACAACTT+GGG - chr2_1:69744286-69744305 Msa0221140:intron 35.0%
! CAAACCAAGGTCTTCTTTTT+AGG - chr2_1:69744341-69744360 Msa0221140:intron 35.0%
!! ACACATAGATTTTGGCATAC+TGG + chr2_1:69744601-69744620 None:intergenic 35.0%
! GTGAATGTTTTGTACTATGG+TGG + chr2_1:69744667-69744686 None:intergenic 35.0%
AGAGAAACACAACCATTCTT+TGG - chr2_1:69744863-69744882 Msa0221140:intron 35.0%
GTCACATCTAGAAAATACAC+TGG + chr2_1:69744959-69744978 None:intergenic 35.0%
ATAACACTACTATACCCTTC+TGG + chr2_1:69745013-69745032 None:intergenic 35.0%
AGAAGAACATTCCAAGTATC+AGG + chr2_1:69745073-69745092 None:intergenic 35.0%
CTTTGGTTGAAGTAGTGAAT+TGG - chr2_1:69745105-69745124 Msa0221140:CDS 35.0%
TGAAGTAGTGAATTGGAAAG+TGG - chr2_1:69745112-69745131 Msa0221140:CDS 35.0%
!! TAGAAAACAATGGGTTTTCC+TGG + chr2_1:69745161-69745180 None:intergenic 35.0%
! AACCCATTGTTTTCTACCAT+AGG - chr2_1:69745165-69745184 Msa0221140:CDS 35.0%
GAGTGTATTTGGTACAATCA+AGG + chr2_1:69745302-69745321 None:intergenic 35.0%
!! GACAACTTTTTCTGCACTCT+TGG - chr2_1:69740874-69740893 Msa0221140:intron 40.0%
! GTCCTTTTCCTTCAACAAAG+AGG - chr2_1:69740896-69740915 Msa0221140:intron 40.0%
GTTTCGATCCTCTTTGTTGA+AGG + chr2_1:69740907-69740926 None:intergenic 40.0%
AGGATCGAAACCCAAGAAAA+TGG - chr2_1:69740916-69740935 Msa0221140:three_prime_UTR 40.0%
GGATCGAAACCCAAGAAAAT+GGG - chr2_1:69740917-69740936 Msa0221140:three_prime_UTR 40.0%
AGTAATGAGAGAAAACTCGC+TGG - chr2_1:69741046-69741065 Msa0221140:three_prime_UTR 40.0%
! TAACTGGTTTCGTGGCAAAT+GGG - chr2_1:69741075-69741094 Msa0221140:CDS 40.0%
GATCATAGAGAGGATACGAA+GGG + chr2_1:69741108-69741127 None:intergenic 40.0%
CAAGAAGAAGGATCATAGAG+AGG + chr2_1:69741118-69741137 None:intergenic 40.0%
TCTATGAAGGGACAAGAAGA+AGG + chr2_1:69741130-69741149 None:intergenic 40.0%
GGAGAAGGAGATATATACCT+TGG + chr2_1:69741235-69741254 None:intergenic 40.0%
ATTCTTGGGTGCCAAAAACA+AGG - chr2_1:69741585-69741604 Msa0221140:intron 40.0%
!! GATAGCACTTTGCTTTGAGA+AGG + chr2_1:69741615-69741634 None:intergenic 40.0%
CAAAGAACACATCACTTGCA+TGG + chr2_1:69741765-69741784 None:intergenic 40.0%
ACAAAACATGGGTTCACACA+AGG + chr2_1:69741831-69741850 None:intergenic 40.0%
TCCATGTTGGTGTGAAACAA+TGG - chr2_1:69741930-69741949 Msa0221140:CDS 40.0%
TCCATTGTTTCACACCAACA+TGG + chr2_1:69741934-69741953 None:intergenic 40.0%
! AAAGTATGAAACGGGTTTGG+TGG - chr2_1:69741965-69741984 Msa0221140:intron 40.0%
TTAAAAATGTCCCCGGCAAA+AGG + chr2_1:69741999-69742018 None:intergenic 40.0%
TGTCTACGTTCCAAAGAAGA+GGG + chr2_1:69742241-69742260 None:intergenic 40.0%
ATGTCTACGTTCCAAAGAAG+AGG + chr2_1:69742242-69742261 None:intergenic 40.0%
!!! GTTTTCTTTTTCGGTCTAGC+TGG + chr2_1:69742472-69742491 None:intergenic 40.0%
AACACGTGTTTCTCAGACTA+TGG - chr2_1:69742489-69742508 Msa0221140:intron 40.0%
ACGTGCAAAGAAGAAAAGGT+TGG + chr2_1:69743082-69743101 None:intergenic 40.0%
CAAAATGGACCACATAACAC+TGG - chr2_1:69743469-69743488 Msa0221140:intron 40.0%
AGGAAAAGTCATGAAAGCAG+AGG + chr2_1:69743515-69743534 None:intergenic 40.0%
TCTATTCCGCACACCATATT+TGG - chr2_1:69743979-69743998 Msa0221140:intron 40.0%
ATGGAGAGGAATGGATGTAT+TGG - chr2_1:69744066-69744085 Msa0221140:intron 40.0%
TGGGATTATATTCAAGAGGG+TGG - chr2_1:69744227-69744246 Msa0221140:intron 40.0%
TTATATTCAAGAGGGTGGCA+AGG - chr2_1:69744232-69744251 Msa0221140:intron 40.0%
CGTTTCATTGCGTTCTACAA+AGG - chr2_1:69744272-69744291 Msa0221140:intron 40.0%
! TTTGACAACTTGGGCTAGAT+GGG - chr2_1:69744295-69744314 Msa0221140:intron 40.0%
TGTAAATCCAGCTCAAACCA+AGG - chr2_1:69744328-69744347 Msa0221140:intron 40.0%
ACAACTTACTCGTAATGCAC+AGG + chr2_1:69744374-69744393 None:intergenic 40.0%
! TCTCTCATGCATGATTTTGC+TGG + chr2_1:69744848-69744867 None:intergenic 40.0%
GGGTACTTCAAACCAAAGAA+TGG + chr2_1:69744878-69744897 None:intergenic 40.0%
TTCACTATTACCCAAGCCAT+TGG + chr2_1:69744911-69744930 None:intergenic 40.0%
AGAAAAGATGCACATCCAGA+AGG - chr2_1:69744995-69745014 Msa0221140:CDS 40.0%
GAAAAGATGCACATCCAGAA+GGG - chr2_1:69744996-69745015 Msa0221140:CDS 40.0%
TGCCAACAGATTGTAGTCAT+TGG - chr2_1:69745032-69745051 Msa0221140:CDS 40.0%
CACCAATGACTACAATCTGT+TGG + chr2_1:69745037-69745056 None:intergenic 40.0%
GTATTTGGTACAATCAAGGC+AGG + chr2_1:69745298-69745317 None:intergenic 40.0%
! GGTAGTTGTTGCTGTTGTGT+TGG + chr2_1:69741014-69741033 None:intergenic 45.0%
AACTCGCTGGAAGATGTAAC+TGG - chr2_1:69741059-69741078 Msa0221140:exon 45.0%
! GGAAGATGTAACTGGTTTCG+TGG - chr2_1:69741067-69741086 Msa0221140:exon 45.0%
! GTAACTGGTTTCGTGGCAAA+TGG - chr2_1:69741074-69741093 Msa0221140:CDS 45.0%
GGATCATAGAGAGGATACGA+AGG + chr2_1:69741109-69741128 None:intergenic 45.0%
CGCCGCTAGTAACTACAAAA+TGG + chr2_1:69741873-69741892 None:intergenic 45.0%
! GTTACTAGCGGCGAATTTTG+TGG - chr2_1:69741880-69741899 Msa0221140:CDS 45.0%
CGCTCCATCCGTCTTATATT+TGG - chr2_1:69742524-69742543 Msa0221140:intron 45.0%
AATGGACCACATAACACTGG+TGG - chr2_1:69743472-69743491 Msa0221140:intron 45.0%
ATTAGGCCACCAGTGTTATG+TGG + chr2_1:69743481-69743500 None:intergenic 45.0%
! CAGAGGAGTGCATCTGAATT+AGG + chr2_1:69743498-69743517 None:intergenic 45.0%
TCACTGAAAGGAGTTGAGTG+AGG - chr2_1:69743676-69743695 Msa0221140:intron 45.0%
GGTCTACCAAATATGGTGTG+CGG + chr2_1:69743988-69744007 None:intergenic 45.0%
CGGTCAAGGTCTACCAAATA+TGG + chr2_1:69743995-69744014 None:intergenic 45.0%
ACCTTGACCGTGAGAACATT+GGG - chr2_1:69744005-69744024 Msa0221140:intron 45.0%
!! CATTGGGCGTGTTCTAAAGT+TGG - chr2_1:69744021-69744040 Msa0221140:intron 45.0%
! AAGAGTGGTAATGCATGGAG+AGG - chr2_1:69744052-69744071 Msa0221140:intron 45.0%
! TGGTAATGCATGGAGAGGAA+TGG - chr2_1:69744057-69744076 Msa0221140:intron 45.0%
TGGTCTTCAACACATGGCAT+TGG - chr2_1:69744086-69744105 Msa0221140:intron 45.0%
! TCTTCAACACATGGCATTGG+TGG - chr2_1:69744089-69744108 Msa0221140:intron 45.0%
! GTTTGACAACTTGGGCTAGA+TGG - chr2_1:69744294-69744313 Msa0221140:intron 45.0%
! AAGAAGACCTTGGTTTGAGC+TGG + chr2_1:69744338-69744357 None:intergenic 45.0%
ACTTGCGCAAAGTATGTGTG+AGG - chr2_1:69744537-69744556 Msa0221140:CDS 45.0%
! GTGGTGGCACACATAGATTT+TGG + chr2_1:69744609-69744628 None:intergenic 45.0%
GGTAAATTAGGGGGAGAGAT+TGG - chr2_1:69744816-69744835 Msa0221140:intron 45.0%
!! TTTGGTTTGAAGTACCCAGC+AGG - chr2_1:69744881-69744900 Msa0221140:intron 45.0%
! TGTAGTCATTGGTGTCTTCC+AGG - chr2_1:69745043-69745062 Msa0221140:CDS 45.0%
TTCCAGGACTTCCTGATACT+TGG - chr2_1:69745059-69745078 Msa0221140:CDS 45.0%
TTCCAAGTATCAGGAAGTCC+TGG + chr2_1:69745064-69745083 None:intergenic 45.0%
CTTCTTCATGCAGCTCTCTT+TGG - chr2_1:69745088-69745107 Msa0221140:CDS 45.0%
!! TATTTAAAAAATACATTTAT+TGG - chr2_1:69742172-69742191 Msa0221140:CDS 5.0%
!! ATTTAAAAAATACATTTATT+GGG - chr2_1:69742173-69742192 Msa0221140:CDS 5.0%
!!! ATATTTAATTTTTCTTTTTT+TGG - chr2_1:69742288-69742307 Msa0221140:intron 5.0%
!!! TATTAATATTATTTTCAAAA+AGG + chr2_1:69743369-69743388 None:intergenic 5.0%
!! GTATGAAACGGGTTTGGTGG+AGG - chr2_1:69741968-69741987 Msa0221140:intron 50.0%
! GTGGAGGCTTTTCCTTTTGC+CGG - chr2_1:69741984-69742003 Msa0221140:intron 50.0%
! TGGAGGCTTTTCCTTTTGCC+GGG - chr2_1:69741985-69742004 Msa0221140:intron 50.0%
GGGGACGTGCAAAGAAGAAA+AGG + chr2_1:69743086-69743105 None:intergenic 50.0%
GACCTTGACCGTGAGAACAT+TGG - chr2_1:69744004-69744023 Msa0221140:intron 50.0%
GAACACGCCCAATGTTCTCA+CGG + chr2_1:69744015-69744034 None:intergenic 50.0%
CGGTTGTGCATTTCCAGTGT+GGG + chr2_1:69744116-69744135 None:intergenic 50.0%
ACGGTTGTGCATTTCCAGTG+TGG + chr2_1:69744117-69744136 None:intergenic 50.0%
CACTGGAAATGCACAACCGT+AGG - chr2_1:69744117-69744136 Msa0221140:intron 50.0%
! AAGCCATTGGTGTTCCTGCT+GGG + chr2_1:69744898-69744917 None:intergenic 50.0%
AGCAGGAACACCAATGGCTT+GGG - chr2_1:69744898-69744917 Msa0221140:intron 50.0%
! GGAGGCTTTTCCTTTTGCCG+GGG - chr2_1:69741986-69742005 Msa0221140:intron 55.0%
GCCCAATGTTCTCACGGTCA+AGG + chr2_1:69744009-69744028 None:intergenic 55.0%
GTACCCAGCAGGAACACCAA+TGG - chr2_1:69744892-69744911 Msa0221140:intron 55.0%
! CAAGCCATTGGTGTTCCTGC+TGG + chr2_1:69744899-69744918 None:intergenic 55.0%
CAGCAGGAACACCAATGGCT+TGG - chr2_1:69744897-69744916 Msa0221140:intron 55.0%
!! TGGCATTGGTGGACCCACAC+TGG - chr2_1:69744100-69744119 Msa0221140:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 69740840 69745348 69740840 ID=Msa0221140;Name=Msa0221140
chr2_1 mRNA 69740840 69745348 69740840 ID=Msa0221140-mRNA-1;Parent=Msa0221140;Name=Msa0221140-mRNA-1;_AED=0.09;_eAED=0.09;_QI=95|1|1|1|0.8|0.66|6|206|365
chr2_1 exon 69740840 69740870 69740840 ID=Msa0221140-mRNA-1:exon:19800;Parent=Msa0221140-mRNA-1
chr2_1 exon 69740899 69741362 69740899 ID=Msa0221140-mRNA-1:exon:19799;Parent=Msa0221140-mRNA-1
chr2_1 exon 69741804 69741962 69741804 ID=Msa0221140-mRNA-1:exon:19798;Parent=Msa0221140-mRNA-1
chr2_1 exon 69742054 69742229 69742054 ID=Msa0221140-mRNA-1:exon:19797;Parent=Msa0221140-mRNA-1
chr2_1 exon 69744536 69744707 69744536 ID=Msa0221140-mRNA-1:exon:19796;Parent=Msa0221140-mRNA-1
chr2_1 exon 69744952 69745348 69744952 ID=Msa0221140-mRNA-1:exon:19795;Parent=Msa0221140-mRNA-1
chr2_1 five_prime_UTR 69745254 69745348 69745254 ID=Msa0221140-mRNA-1:five_prime_utr;Parent=Msa0221140-mRNA-1
chr2_1 CDS 69744952 69745253 69744952 ID=Msa0221140-mRNA-1:cds;Parent=Msa0221140-mRNA-1
chr2_1 CDS 69744536 69744707 69744536 ID=Msa0221140-mRNA-1:cds;Parent=Msa0221140-mRNA-1
chr2_1 CDS 69742054 69742229 69742054 ID=Msa0221140-mRNA-1:cds;Parent=Msa0221140-mRNA-1
chr2_1 CDS 69741804 69741962 69741804 ID=Msa0221140-mRNA-1:cds;Parent=Msa0221140-mRNA-1
chr2_1 CDS 69741074 69741362 69741074 ID=Msa0221140-mRNA-1:cds;Parent=Msa0221140-mRNA-1
chr2_1 three_prime_UTR 69740899 69741073 69740899 ID=Msa0221140-mRNA-1:three_prime_utr;Parent=Msa0221140-mRNA-1
chr2_1 three_prime_UTR 69740840 69740870 69740840 ID=Msa0221140-mRNA-1:three_prime_utr;Parent=Msa0221140-mRNA-1
Gene Sequence

>Msa0221140

ATGGGTTTTCTGTTACCGACTCTGTTTTTACTTCTTCTGTTTTCTTCACACCAAACAAAAGCTCTTGAATTTAACTCCAACACAACAGCAACAACTACCATTTCCAGTAGTAGTAATGAGAGAAAACTCGCTGGAAGATGTAACTGGTTTCGTGGCAAATGGGTCTATGACCCTTCGTATCCTCTCTATGATCCTTCTTCTTGTCCCTTCATAGATCCACAATTCAATTGTCAAAAGTATGGCAGACCTGATACTCAGTATCAGAAATATAGATGGCAACCTTTCACTTGTTCTATACCAAGGTTCAATGCATTGGATTTTCTAGCAAAGTACAGGGGAAAAAAGATTATGTTTGTTGGTGATTCTTTGAGCTTAAACCAGTTCAATTCACTAGCATGTATGATTCATTCTTGGGTGCCAAAAACAAGGTATTCCTTCTCAAAGCAAAGTGCTATCTCCACAATAACATTTCAGGATTATGGTCTCCAATTATTTCTATTCCGCACACCATATTTGGTAGACCTTGACCGTGAGAACATTGGGCGTGTTCTAAAGTTGGACTCAATAAAGAGTGGTAATGCATGGAGAGGAATGGATGTATTGGTCTTCAACACATGGCATTGGTGGACCCACACTGGAAATGCACAACCATGGGATTATATTCAAGAGGGTGGCAAGGTGTACAAAGACATGAACCGTTTCATTGCGTTCTACAAAGGTTTGACAACTTGGGCTAGATGGGTTAACATCAATGTAAATCCAGCTCAAACCAAGGTCTTCTTTTTAGGAATTTCTCCTGTGCATTACGAGGGGAGAGATTGGAATCAACCAGCAAAATCATGCATGAGAGAAACACAACCATTCTTTGGTTTGAAGTACCCAGCAGGAACACCAATGGCTTGGGTAATAGTGAATAAAGTATTGAGCAGATTAAAGAAGCCAGTGTATTTTCTAGATGTGACAACATTGTCACAGTACAGAAAAGATGCACATCCAGAAGGGTATAGTAGTGTTATGCCAACAGATTGTAGTCATTGGTGTCTTCCAGGACTTCCTGATACTTGGAATGTTCTTCTTCATGCAGCTCTCTTTGGTTGA
Protein sequence

>Msa0221140

MGFLLPTLFLLLLFSSHQTKALEFNSNTTATTTISSSSNERKLAGRCNWFRGKWVYDPSYPLYDPSSCPFIDPQFNCQKYGRPDTQYQKYRWQPFTCSIPRFNALDFLAKYRGKKIMFVGDSLSLNQFNSLACMIHSWVPKTRYSFSKQSAISTITFQDYGLQLFLFRTPYLVDLDRENIGRVLKLDSIKSGNAWRGMDVLVFNTWHWWTHTGNAQPWDYIQEGGKVYKDMNRFIAFYKGLTTWARWVNINVNPAQTKVFFLGISPVHYEGRDWNQPAKSCMRETQPFFGLKYPAGTPMAWVIVNKVLSRLKKPVYFLDVTTLSQYRKDAHPEGYSSVMPTDCSHWCLPGLPDTWNVLLHAALFG*