AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0346110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0346110 MtrunA17_Chr3g0078561 89.655 406 17 1 1 381 1 406 0.0 726
Msa0346110 MtrunA17_Chr8g0356011 36.613 437 191 16 1 377 1 411 3.25e-51 176
Msa0346110 MtrunA17_Chr5g0440311 36.735 98 60 2 11 108 4 99 1.10e-13 70.5
Msa0346110 MtrunA17_Chr5g0440291 31.655 139 74 5 14 142 8 135 3.61e-13 68.9
Msa0346110 MtrunA17_Chr3g0078491 27.970 404 230 18 20 378 9 396 2.41e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0346110 AT5G04890.1 44.944 89 48 1 20 108 16 103 1.73e-19 89.4
Msa0346110 AT2G27140.1 33.607 122 78 3 17 138 11 129 1.00e-14 73.2
Msa0346110 AT3G10680.1 33.636 110 69 2 1 107 1 109 7.11e-13 70.1

Find 78 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCGAACACTAAGGATTTC+TGG 0.223242 3_1:+6035324 Msa0346110:CDS
CTTCAAGGCAAGTTTGAAAA+TGG 0.223575 3_1:+6035429 Msa0346110:CDS
AAAGTTTCATACACAGGATT+TGG 0.240469 3_1:-6034660 None:intergenic
AAAGCCCTTAGTTGCAAATA+TGG 0.256931 3_1:+6036202 Msa0346110:CDS
TTGGTTCCCTTGGGTGTTCT+AGG 0.258214 3_1:-6035534 None:intergenic
TTTCTTCACGTTCACCTTCC+TGG 0.265041 3_1:+6034723 Msa0346110:CDS
ACCTTCTTGTCTCTAATTAT+AGG 0.272544 3_1:-6035594 None:intergenic
GCTAGAAGAGGTAAACTTAT+AGG 0.274512 3_1:-6036267 None:intergenic
CCCTTGGGTGTTCTAGGACT+TGG 0.288101 3_1:-6035528 None:intergenic
ATCAAAGGGAGAAGAATATT+TGG 0.290042 3_1:+6036016 Msa0346110:CDS
GAATAAATCAAGAAGCAATA+AGG 0.300890 3_1:+6034589 Msa0346110:five_prime_UTR
AAGTTTCATACACAGGATTT+GGG 0.304860 3_1:-6034659 None:intergenic
TGGAGGCTTTGAAGGAATAT+TGG 0.320768 3_1:-6035553 None:intergenic
TCCAGAAATCCTTAGTGTTC+GGG 0.322505 3_1:-6035325 None:intergenic
TTTAGAAGGAAAAGAAATAA+AGG 0.334192 3_1:+6035980 Msa0346110:CDS
CTCCAGAAATCCTTAGTGTT+CGG 0.335273 3_1:-6035326 None:intergenic
TTTGACTTGGAAAAGTATCT+TGG 0.347158 3_1:-6035686 None:intergenic
TCAAGAAGCAATAAGGGATA+TGG 0.352863 3_1:+6034596 Msa0346110:exon
TATATCCAACATCTGTTCAT+TGG 0.369391 3_1:+6034549 Msa0346110:five_prime_UTR
TGAAGGAATATTGGTTCCCT+TGG 0.376353 3_1:-6035544 None:intergenic
AACAAGTTGAAGGAACAAAA+AGG 0.378553 3_1:+6035660 Msa0346110:CDS
CCAACCCAAGTCAGAATTGA+AGG 0.386552 3_1:+6034683 Msa0346110:CDS
AGCACCCATATTTGCAACTA+AGG 0.387876 3_1:-6036207 None:intergenic
ACTCTAGTTGGAGGCTTTGA+AGG 0.393913 3_1:-6035561 None:intergenic
TCACTCTTCTATTAATATAA+TGG 0.395882 3_1:+6036388 Msa0346110:three_prime_UTR
AATAAATCAAGAAGCAATAA+GGG 0.424439 3_1:+6034590 Msa0346110:five_prime_UTR
CCTTCAATTCTGACTTGGGT+TGG 0.424665 3_1:-6034683 None:intergenic
AACCCTGTCACAATATTACT+TGG 0.426130 3_1:-6035627 None:intergenic
GAAGGAATATTGGTTCCCTT+GGG 0.429823 3_1:-6035543 None:intergenic
TCTCCAAGTAATATTGTGAC+AGG 0.442725 3_1:+6035624 Msa0346110:CDS
GAGGATAAATATCAAATTTG+TGG 0.447215 3_1:+6035296 Msa0346110:CDS
TTTGAATTACATTTCAAGAA+AGG 0.448423 3_1:-6036330 None:intergenic
TTGACTTGGAAAAGTATCTT+GGG 0.454151 3_1:-6035685 None:intergenic
TCTCAAGTTGTCAAGAGAAT+AGG 0.458977 3_1:+6036155 Msa0346110:CDS
GGTTCTTCTAGTACTCTAGT+TGG 0.460763 3_1:-6035573 None:intergenic
AGGATAAATATCAAATTTGT+GGG 0.471872 3_1:+6035297 Msa0346110:CDS
TAGACCTCAAAATGTTTATG+AGG 0.473198 3_1:+6035752 Msa0346110:CDS
AAAAGATGCAAAGAAGGAAA+AGG 0.477137 3_1:+6036043 Msa0346110:CDS
TGAGGTCTAGATTCTACAAA+GGG 0.477686 3_1:-6035738 None:intergenic
TCTTCTAGTACTCTAGTTGG+AGG 0.480814 3_1:-6035570 None:intergenic
GGCTCCAAAAGATGCAAAGA+AGG 0.483316 3_1:+6036037 Msa0346110:CDS
TTTCTGGAACAGGATATGAT+TGG 0.485095 3_1:-6035389 None:intergenic
TGAAAAGTATACAAAAGAGA+AGG 0.489401 3_1:+6036103 Msa0346110:CDS
TGTGAGGTGGAGAAACTTCA+AGG 0.500541 3_1:+6035414 Msa0346110:CDS
AACTACTTGGTAGCAACAAA+TGG 0.500941 3_1:+6035355 Msa0346110:CDS
CACTAAGGATTTCTGGAGAA+AGG 0.501873 3_1:+6035331 Msa0346110:CDS
TCTGGAGAAAGGCAACTACT+TGG 0.505666 3_1:+6035342 Msa0346110:CDS
ATTCTAGTAATTGTTGCACT+TGG 0.506438 3_1:+6036233 Msa0346110:CDS
GGCAAGAAAAGAGTCATCAA+AGG 0.512092 3_1:+6036001 Msa0346110:CDS
AAGCCCTTAGTTGCAAATAT+GGG 0.513631 3_1:+6036203 Msa0346110:CDS
ACAATATTACTTGGAGATGA+AGG 0.514944 3_1:-6035618 None:intergenic
CAACTTGTGATTTAAGTGAT+GGG 0.517094 3_1:-6035482 None:intergenic
TTTCCAAGTCAAAGTCCTAA+GGG 0.518428 3_1:+6035696 Msa0346110:CDS
GTCAGAATTGAAGGAAAATG+AGG 0.520642 3_1:+6034692 Msa0346110:CDS
GCAAGAAAAGAGTCATCAAA+GGG 0.524808 3_1:+6036002 Msa0346110:CDS
AAATGACTTGAACAAGTTGA+AGG 0.526624 3_1:+6035650 Msa0346110:CDS
ACCTATAATTAGAGACAAGA+AGG 0.529275 3_1:+6035593 Msa0346110:CDS
TCATAGTAATTAGTAGTACT+CGG 0.542897 3_1:+6036411 Msa0346110:three_prime_UTR
TGGGACTCTCCCGAACACTA+AGG 0.543028 3_1:+6035316 Msa0346110:CDS
CAAAGCAAAGAGGTATAAGA+AGG 0.543303 3_1:+6036079 Msa0346110:CDS
GAAGGGAATCTTCAAAGAAG+TGG 0.544582 3_1:+6036121 Msa0346110:CDS
TTTCCCTTAGGACTTTGACT+TGG 0.546496 3_1:-6035699 None:intergenic
GAAAAGTATACAAAAGAGAA+GGG 0.549687 3_1:+6036104 Msa0346110:CDS
TCAACTTGTGATTTAAGTGA+TGG 0.550862 3_1:-6035483 None:intergenic
GCACCCATATTTGCAACTAA+GGG 0.560380 3_1:-6036206 None:intergenic
GGTTGGAAAGTTTCATACAC+AGG 0.568545 3_1:-6034666 None:intergenic
GATCACCAATGAACAGATGT+TGG 0.586607 3_1:-6034554 None:intergenic
CATAGTAATTAGTAGTACTC+GGG 0.587325 3_1:+6036412 Msa0346110:three_prime_UTR
TTGAGGTCTAGATTCTACAA+AGG 0.598222 3_1:-6035739 None:intergenic
GCCAAGTCCTAGAACACCCA+AGG 0.612732 3_1:+6035527 Msa0346110:CDS
GTTGTCAAGAGAATAGGAGA+AGG 0.616928 3_1:+6036161 Msa0346110:CDS
CTACAATTGACAAAGCAAAG+AGG 0.640484 3_1:+6036069 Msa0346110:CDS
AGTTTCATACACAGGATTTG+GGG 0.640952 3_1:-6034658 None:intergenic
CTCCAAGTAATATTGTGACA+GGG 0.641452 3_1:+6035625 Msa0346110:CDS
TGTTCCAGAAAATTGTGAGG+TGG 0.660896 3_1:+6035401 Msa0346110:CDS
TCCTGTTCCAGAAAATTGTG+AGG 0.672952 3_1:+6035398 Msa0346110:CDS
ATTATATTAATAGAAGAGTG+AGG 0.689619 3_1:-6036386 None:intergenic
CCAAGTCCTAGAACACCCAA+GGG 0.690447 3_1:+6035528 Msa0346110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATGAGTTTTTTTTTTTT+TGG + chr3_1:6034829-6034848 Msa0346110:intron 10.0%
!!! ATGAAATTGATAATTATAAA+AGG + chr3_1:6034929-6034948 Msa0346110:intron 10.0%
!!! TTTATGAGTTTTTTTTTTTT+TGG + chr3_1:6034829-6034848 Msa0346110:intron 10.0%
!!! ATGAAATTGATAATTATAAA+AGG + chr3_1:6034929-6034948 Msa0346110:intron 10.0%
!! ATGCAACAAAAAAAAAAAAT+TGG - chr3_1:6034883-6034902 None:intergenic 15.0%
!!! TTTTTTAATTGCTTCTAATA+TGG - chr3_1:6035850-6035869 None:intergenic 15.0%
!! ATGCAACAAAAAAAAAAAAT+TGG - chr3_1:6034883-6034902 None:intergenic 15.0%
!!! TTTTTTAATTGCTTCTAATA+TGG - chr3_1:6035850-6035869 None:intergenic 15.0%
!! AATAAATCAAGAAGCAATAA+GGG + chr3_1:6034590-6034609 Msa0346110:five_prime_UTR 20.0%
!! TCAACTAAAGTTAATGTATT+CGG - chr3_1:6035158-6035177 None:intergenic 20.0%
!! TCTTTGTAAAAAAGATTTGA+GGG + chr3_1:6035186-6035205 Msa0346110:intron 20.0%
!! AGGATAAATATCAAATTTGT+GGG + chr3_1:6035297-6035316 Msa0346110:CDS 20.0%
!! AAAAAATTCAAGAGGAAAAT+AGG + chr3_1:6035817-6035836 Msa0346110:CDS 20.0%
!! TTTAGAAGGAAAAGAAATAA+AGG + chr3_1:6035980-6035999 Msa0346110:CDS 20.0%
!! TTTGAATTACATTTCAAGAA+AGG - chr3_1:6036333-6036352 None:intergenic 20.0%
!! ATTATATTAATAGAAGAGTG+AGG - chr3_1:6036389-6036408 None:intergenic 20.0%
!! TCACTCTTCTATTAATATAA+TGG + chr3_1:6036388-6036407 Msa0346110:three_prime_UTR 20.0%
!! AATAAATCAAGAAGCAATAA+GGG + chr3_1:6034590-6034609 Msa0346110:five_prime_UTR 20.0%
!! TCAACTAAAGTTAATGTATT+CGG - chr3_1:6035158-6035177 None:intergenic 20.0%
!! TCTTTGTAAAAAAGATTTGA+GGG + chr3_1:6035186-6035205 Msa0346110:intron 20.0%
!! AGGATAAATATCAAATTTGT+GGG + chr3_1:6035297-6035316 Msa0346110:CDS 20.0%
!! AAAAAATTCAAGAGGAAAAT+AGG + chr3_1:6035817-6035836 Msa0346110:CDS 20.0%
!! TTTAGAAGGAAAAGAAATAA+AGG + chr3_1:6035980-6035999 Msa0346110:CDS 20.0%
!! TTTGAATTACATTTCAAGAA+AGG - chr3_1:6036333-6036352 None:intergenic 20.0%
!! ATTATATTAATAGAAGAGTG+AGG - chr3_1:6036389-6036408 None:intergenic 20.0%
!! TCACTCTTCTATTAATATAA+TGG + chr3_1:6036388-6036407 Msa0346110:three_prime_UTR 20.0%
! GAATAAATCAAGAAGCAATA+AGG + chr3_1:6034589-6034608 Msa0346110:five_prime_UTR 25.0%
! CCTATTTATCTCTTACAAAA+AGG - chr3_1:6035099-6035118 None:intergenic 25.0%
!! CCTTTTTGTAAGAGATAAAT+AGG + chr3_1:6035096-6035115 Msa0346110:intron 25.0%
! GTCTTTGTAAAAAAGATTTG+AGG + chr3_1:6035185-6035204 Msa0346110:intron 25.0%
! GAGGATAAATATCAAATTTG+TGG + chr3_1:6035296-6035315 Msa0346110:CDS 25.0%
!!! TTAAGTGATGGGAATTTTTT+TGG - chr3_1:6035474-6035493 None:intergenic 25.0%
!! TTTTCCTCATAAACATTTTG+AGG - chr3_1:6035759-6035778 None:intergenic 25.0%
!! TTTTTCATCATCAATTGCTT+GGG - chr3_1:6035802-6035821 None:intergenic 25.0%
!!! TTTTTTCATCATCAATTGCT+TGG - chr3_1:6035803-6035822 None:intergenic 25.0%
!! TGATGATGAAAAAATTCAAG+AGG + chr3_1:6035809-6035828 Msa0346110:CDS 25.0%
! AATTAAAAAACAATTGCACG+AGG + chr3_1:6035860-6035879 Msa0346110:CDS 25.0%
!! TGAAAAGTATACAAAAGAGA+AGG + chr3_1:6036103-6036122 Msa0346110:CDS 25.0%
!! GAAAAGTATACAAAAGAGAA+GGG + chr3_1:6036104-6036123 Msa0346110:CDS 25.0%
! TCATAGTAATTAGTAGTACT+CGG + chr3_1:6036411-6036430 Msa0346110:three_prime_UTR 25.0%
! GAATAAATCAAGAAGCAATA+AGG + chr3_1:6034589-6034608 Msa0346110:five_prime_UTR 25.0%
! CCTATTTATCTCTTACAAAA+AGG - chr3_1:6035099-6035118 None:intergenic 25.0%
!! CCTTTTTGTAAGAGATAAAT+AGG + chr3_1:6035096-6035115 Msa0346110:intron 25.0%
! GTCTTTGTAAAAAAGATTTG+AGG + chr3_1:6035185-6035204 Msa0346110:intron 25.0%
! GAGGATAAATATCAAATTTG+TGG + chr3_1:6035296-6035315 Msa0346110:CDS 25.0%
!!! TTAAGTGATGGGAATTTTTT+TGG - chr3_1:6035474-6035493 None:intergenic 25.0%
!! TTTTCCTCATAAACATTTTG+AGG - chr3_1:6035759-6035778 None:intergenic 25.0%
!! TTTTTCATCATCAATTGCTT+GGG - chr3_1:6035802-6035821 None:intergenic 25.0%
!!! TTTTTTCATCATCAATTGCT+TGG - chr3_1:6035803-6035822 None:intergenic 25.0%
!! TGATGATGAAAAAATTCAAG+AGG + chr3_1:6035809-6035828 Msa0346110:CDS 25.0%
! AATTAAAAAACAATTGCACG+AGG + chr3_1:6035860-6035879 Msa0346110:CDS 25.0%
!! TGAAAAGTATACAAAAGAGA+AGG + chr3_1:6036103-6036122 Msa0346110:CDS 25.0%
!! GAAAAGTATACAAAAGAGAA+GGG + chr3_1:6036104-6036123 Msa0346110:CDS 25.0%
! TCATAGTAATTAGTAGTACT+CGG + chr3_1:6036411-6036430 Msa0346110:three_prime_UTR 25.0%
TATATCCAACATCTGTTCAT+TGG + chr3_1:6034549-6034568 Msa0346110:five_prime_UTR 30.0%
AAGTTTCATACACAGGATTT+GGG - chr3_1:6034662-6034681 None:intergenic 30.0%
AAAGTTTCATACACAGGATT+TGG - chr3_1:6034663-6034682 None:intergenic 30.0%
! TTTTCCTTCAATTCTGACTT+GGG - chr3_1:6034690-6034709 None:intergenic 30.0%
! ATTTTCCTTCAATTCTGACT+TGG - chr3_1:6034691-6034710 None:intergenic 30.0%
AAAAACAAAAAATACGCACC+AGG - chr3_1:6034744-6034763 None:intergenic 30.0%
AAATTGGATCCTTTCTAATG+TGG - chr3_1:6034867-6034886 None:intergenic 30.0%
TCTCTTACAAAAAGGACTAA+AGG - chr3_1:6035091-6035110 None:intergenic 30.0%
ATTTGAGGGAGTTAAATTTG+AGG + chr3_1:6035200-6035219 Msa0346110:intron 30.0%
!! TGTTCTGCAAGGTTTTATAA+AGG + chr3_1:6035272-6035291 Msa0346110:intron 30.0%
CAACTTGTGATTTAAGTGAT+GGG - chr3_1:6035485-6035504 None:intergenic 30.0%
TCAACTTGTGATTTAAGTGA+TGG - chr3_1:6035486-6035505 None:intergenic 30.0%
!!! ACTTGGCACAATTTTTTCTT+TGG - chr3_1:6035514-6035533 None:intergenic 30.0%
ACCTATAATTAGAGACAAGA+AGG + chr3_1:6035593-6035612 Msa0346110:CDS 30.0%
ACCTTCTTGTCTCTAATTAT+AGG - chr3_1:6035597-6035616 None:intergenic 30.0%
ACAATATTACTTGGAGATGA+AGG - chr3_1:6035621-6035640 None:intergenic 30.0%
! AAATGACTTGAACAAGTTGA+AGG + chr3_1:6035650-6035669 Msa0346110:CDS 30.0%
! AACAAGTTGAAGGAACAAAA+AGG + chr3_1:6035660-6035679 Msa0346110:CDS 30.0%
!! TTGACTTGGAAAAGTATCTT+GGG - chr3_1:6035688-6035707 None:intergenic 30.0%
!! TTTGACTTGGAAAAGTATCT+TGG - chr3_1:6035689-6035708 None:intergenic 30.0%
! AAAGCTTTCAATTTTCCCTT+AGG - chr3_1:6035714-6035733 None:intergenic 30.0%
TAGACCTCAAAATGTTTATG+AGG + chr3_1:6035752-6035771 Msa0346110:CDS 30.0%
!! GATCACAATACGTTTTTAGA+AGG + chr3_1:6035966-6035985 Msa0346110:CDS 30.0%
ATCAAAGGGAGAAGAATATT+TGG + chr3_1:6036016-6036035 Msa0346110:CDS 30.0%
! TTTTCCTTCTTTGCATCTTT+TGG - chr3_1:6036044-6036063 None:intergenic 30.0%
AAAAGATGCAAAGAAGGAAA+AGG + chr3_1:6036043-6036062 Msa0346110:CDS 30.0%
ATTCTAGTAATTGTTGCACT+TGG + chr3_1:6036233-6036252 Msa0346110:CDS 30.0%
!!! GTTTTAGTTTTTGCTAGAAG+AGG - chr3_1:6036282-6036301 None:intergenic 30.0%
CATAGTAATTAGTAGTACTC+GGG + chr3_1:6036412-6036431 Msa0346110:three_prime_UTR 30.0%
ATGAACAGATGTTGGATATA+AGG - chr3_1:6034549-6034568 None:intergenic 30.0%
TATATCCAACATCTGTTCAT+TGG + chr3_1:6034549-6034568 Msa0346110:five_prime_UTR 30.0%
AAGTTTCATACACAGGATTT+GGG - chr3_1:6034662-6034681 None:intergenic 30.0%
AAAGTTTCATACACAGGATT+TGG - chr3_1:6034663-6034682 None:intergenic 30.0%
! TTTTCCTTCAATTCTGACTT+GGG - chr3_1:6034690-6034709 None:intergenic 30.0%
! ATTTTCCTTCAATTCTGACT+TGG - chr3_1:6034691-6034710 None:intergenic 30.0%
AAAAACAAAAAATACGCACC+AGG - chr3_1:6034744-6034763 None:intergenic 30.0%
AAATTGGATCCTTTCTAATG+TGG - chr3_1:6034867-6034886 None:intergenic 30.0%
TCTCTTACAAAAAGGACTAA+AGG - chr3_1:6035091-6035110 None:intergenic 30.0%
ATTTGAGGGAGTTAAATTTG+AGG + chr3_1:6035200-6035219 Msa0346110:intron 30.0%
!! TGTTCTGCAAGGTTTTATAA+AGG + chr3_1:6035272-6035291 Msa0346110:intron 30.0%
CAACTTGTGATTTAAGTGAT+GGG - chr3_1:6035485-6035504 None:intergenic 30.0%
TCAACTTGTGATTTAAGTGA+TGG - chr3_1:6035486-6035505 None:intergenic 30.0%
!!! ACTTGGCACAATTTTTTCTT+TGG - chr3_1:6035514-6035533 None:intergenic 30.0%
ACCTATAATTAGAGACAAGA+AGG + chr3_1:6035593-6035612 Msa0346110:CDS 30.0%
ACCTTCTTGTCTCTAATTAT+AGG - chr3_1:6035597-6035616 None:intergenic 30.0%
ACAATATTACTTGGAGATGA+AGG - chr3_1:6035621-6035640 None:intergenic 30.0%
! AAATGACTTGAACAAGTTGA+AGG + chr3_1:6035650-6035669 Msa0346110:CDS 30.0%
! AACAAGTTGAAGGAACAAAA+AGG + chr3_1:6035660-6035679 Msa0346110:CDS 30.0%
!! TTGACTTGGAAAAGTATCTT+GGG - chr3_1:6035688-6035707 None:intergenic 30.0%
!! TTTGACTTGGAAAAGTATCT+TGG - chr3_1:6035689-6035708 None:intergenic 30.0%
! AAAGCTTTCAATTTTCCCTT+AGG - chr3_1:6035714-6035733 None:intergenic 30.0%
TAGACCTCAAAATGTTTATG+AGG + chr3_1:6035752-6035771 Msa0346110:CDS 30.0%
!! GATCACAATACGTTTTTAGA+AGG + chr3_1:6035966-6035985 Msa0346110:CDS 30.0%
ATCAAAGGGAGAAGAATATT+TGG + chr3_1:6036016-6036035 Msa0346110:CDS 30.0%
! TTTTCCTTCTTTGCATCTTT+TGG - chr3_1:6036044-6036063 None:intergenic 30.0%
AAAAGATGCAAAGAAGGAAA+AGG + chr3_1:6036043-6036062 Msa0346110:CDS 30.0%
ATTCTAGTAATTGTTGCACT+TGG + chr3_1:6036233-6036252 Msa0346110:CDS 30.0%
!!! GTTTTAGTTTTTGCTAGAAG+AGG - chr3_1:6036282-6036301 None:intergenic 30.0%
CATAGTAATTAGTAGTACTC+GGG + chr3_1:6036412-6036431 Msa0346110:three_prime_UTR 30.0%
TCAAGAAGCAATAAGGGATA+TGG + chr3_1:6034596-6034615 Msa0346110:exon 35.0%
AGTTTCATACACAGGATTTG+GGG - chr3_1:6034661-6034680 None:intergenic 35.0%
GTCAGAATTGAAGGAAAATG+AGG + chr3_1:6034692-6034711 Msa0346110:CDS 35.0%
!! TGCAAGGTTTTATAAAGGAG+AGG + chr3_1:6035277-6035296 Msa0346110:intron 35.0%
! AACTACTTGGTAGCAACAAA+TGG + chr3_1:6035355-6035374 Msa0346110:CDS 35.0%
!! TTTCTGGAACAGGATATGAT+TGG - chr3_1:6035392-6035411 None:intergenic 35.0%
CTTCAAGGCAAGTTTGAAAA+TGG + chr3_1:6035429-6035448 Msa0346110:CDS 35.0%
TCTCCAAGTAATATTGTGAC+AGG + chr3_1:6035624-6035643 Msa0346110:CDS 35.0%
CTCCAAGTAATATTGTGACA+GGG + chr3_1:6035625-6035644 Msa0346110:CDS 35.0%
AACCCTGTCACAATATTACT+TGG - chr3_1:6035630-6035649 None:intergenic 35.0%
! TTTTCCAAGTCAAAGTCCTA+AGG + chr3_1:6035695-6035714 Msa0346110:CDS 35.0%
TTTCCAAGTCAAAGTCCTAA+GGG + chr3_1:6035696-6035715 Msa0346110:CDS 35.0%
TGAGGTCTAGATTCTACAAA+GGG - chr3_1:6035741-6035760 None:intergenic 35.0%
TTGAGGTCTAGATTCTACAA+AGG - chr3_1:6035742-6035761 None:intergenic 35.0%
! ATTGCTTGGGATTTTGCTTT+TGG - chr3_1:6035789-6035808 None:intergenic 35.0%
GCAAGAAAAGAGTCATCAAA+GGG + chr3_1:6036002-6036021 Msa0346110:CDS 35.0%
CTACAATTGACAAAGCAAAG+AGG + chr3_1:6036069-6036088 Msa0346110:CDS 35.0%
! CAAAGCAAAGAGGTATAAGA+AGG + chr3_1:6036079-6036098 Msa0346110:CDS 35.0%
! TCTCAAGTTGTCAAGAGAAT+AGG + chr3_1:6036155-6036174 Msa0346110:CDS 35.0%
AAAGCCCTTAGTTGCAAATA+TGG + chr3_1:6036202-6036221 Msa0346110:CDS 35.0%
AAGCCCTTAGTTGCAAATAT+GGG + chr3_1:6036203-6036222 Msa0346110:CDS 35.0%
GCTAGAAGAGGTAAACTTAT+AGG - chr3_1:6036270-6036289 None:intergenic 35.0%
TCAAGAAGCAATAAGGGATA+TGG + chr3_1:6034596-6034615 Msa0346110:exon 35.0%
AGTTTCATACACAGGATTTG+GGG - chr3_1:6034661-6034680 None:intergenic 35.0%
GTCAGAATTGAAGGAAAATG+AGG + chr3_1:6034692-6034711 Msa0346110:CDS 35.0%
!! TGCAAGGTTTTATAAAGGAG+AGG + chr3_1:6035277-6035296 Msa0346110:intron 35.0%
! AACTACTTGGTAGCAACAAA+TGG + chr3_1:6035355-6035374 Msa0346110:CDS 35.0%
!! TTTCTGGAACAGGATATGAT+TGG - chr3_1:6035392-6035411 None:intergenic 35.0%
CTTCAAGGCAAGTTTGAAAA+TGG + chr3_1:6035429-6035448 Msa0346110:CDS 35.0%
TCTCCAAGTAATATTGTGAC+AGG + chr3_1:6035624-6035643 Msa0346110:CDS 35.0%
CTCCAAGTAATATTGTGACA+GGG + chr3_1:6035625-6035644 Msa0346110:CDS 35.0%
AACCCTGTCACAATATTACT+TGG - chr3_1:6035630-6035649 None:intergenic 35.0%
! TTTTCCAAGTCAAAGTCCTA+AGG + chr3_1:6035695-6035714 Msa0346110:CDS 35.0%
TTTCCAAGTCAAAGTCCTAA+GGG + chr3_1:6035696-6035715 Msa0346110:CDS 35.0%
TGAGGTCTAGATTCTACAAA+GGG - chr3_1:6035741-6035760 None:intergenic 35.0%
TTGAGGTCTAGATTCTACAA+AGG - chr3_1:6035742-6035761 None:intergenic 35.0%
! ATTGCTTGGGATTTTGCTTT+TGG - chr3_1:6035789-6035808 None:intergenic 35.0%
GCAAGAAAAGAGTCATCAAA+GGG + chr3_1:6036002-6036021 Msa0346110:CDS 35.0%
CTACAATTGACAAAGCAAAG+AGG + chr3_1:6036069-6036088 Msa0346110:CDS 35.0%
! CAAAGCAAAGAGGTATAAGA+AGG + chr3_1:6036079-6036098 Msa0346110:CDS 35.0%
! TCTCAAGTTGTCAAGAGAAT+AGG + chr3_1:6036155-6036174 Msa0346110:CDS 35.0%
AAAGCCCTTAGTTGCAAATA+TGG + chr3_1:6036202-6036221 Msa0346110:CDS 35.0%
AAGCCCTTAGTTGCAAATAT+GGG + chr3_1:6036203-6036222 Msa0346110:CDS 35.0%
GCTAGAAGAGGTAAACTTAT+AGG - chr3_1:6036270-6036289 None:intergenic 35.0%
GATCACCAATGAACAGATGT+TGG - chr3_1:6034557-6034576 None:intergenic 40.0%
!!! GAACGTTTTGTTCTAGAGGT+AGG - chr3_1:6034639-6034658 None:intergenic 40.0%
GGTTGGAAAGTTTCATACAC+AGG - chr3_1:6034669-6034688 None:intergenic 40.0%
ACAAAAAATACGCACCAGGA+AGG - chr3_1:6034740-6034759 None:intergenic 40.0%
TGTACTCCTCCACATTAGAA+AGG + chr3_1:6034855-6034874 Msa0346110:intron 40.0%
TTGGATCCTTTCTAATGTGG+AGG - chr3_1:6034864-6034883 None:intergenic 40.0%
! CTTTTCTCTCATGTTCTGCA+AGG + chr3_1:6035261-6035280 Msa0346110:intron 40.0%
TCCAGAAATCCTTAGTGTTC+GGG - chr3_1:6035328-6035347 None:intergenic 40.0%
CTCCAGAAATCCTTAGTGTT+CGG - chr3_1:6035329-6035348 None:intergenic 40.0%
! CACTAAGGATTTCTGGAGAA+AGG + chr3_1:6035331-6035350 Msa0346110:CDS 40.0%
TCCTGTTCCAGAAAATTGTG+AGG + chr3_1:6035398-6035417 Msa0346110:CDS 40.0%
!!! ACCTCACAATTTTCTGGAAC+AGG - chr3_1:6035402-6035421 None:intergenic 40.0%
TGTTCCAGAAAATTGTGAGG+TGG + chr3_1:6035401-6035420 Msa0346110:CDS 40.0%
! TTCTCCACCTCACAATTTTC+TGG - chr3_1:6035408-6035427 None:intergenic 40.0%
!! GAAGGAATATTGGTTCCCTT+GGG - chr3_1:6035546-6035565 None:intergenic 40.0%
!! TGAAGGAATATTGGTTCCCT+TGG - chr3_1:6035547-6035566 None:intergenic 40.0%
!! TGGAGGCTTTGAAGGAATAT+TGG - chr3_1:6035556-6035575 None:intergenic 40.0%
TCTTCTAGTACTCTAGTTGG+AGG - chr3_1:6035573-6035592 None:intergenic 40.0%
GGTTCTTCTAGTACTCTAGT+TGG - chr3_1:6035576-6035595 None:intergenic 40.0%
TTTCCCTTAGGACTTTGACT+TGG - chr3_1:6035702-6035721 None:intergenic 40.0%
GGCAAGAAAAGAGTCATCAA+AGG + chr3_1:6036001-6036020 Msa0346110:CDS 40.0%
GAAGGGAATCTTCAAAGAAG+TGG + chr3_1:6036121-6036140 Msa0346110:CDS 40.0%
GTTGTCAAGAGAATAGGAGA+AGG + chr3_1:6036161-6036180 Msa0346110:CDS 40.0%
GCACCCATATTTGCAACTAA+GGG - chr3_1:6036209-6036228 None:intergenic 40.0%
AGCACCCATATTTGCAACTA+AGG - chr3_1:6036210-6036229 None:intergenic 40.0%
GATCACCAATGAACAGATGT+TGG - chr3_1:6034557-6034576 None:intergenic 40.0%
!!! GAACGTTTTGTTCTAGAGGT+AGG - chr3_1:6034639-6034658 None:intergenic 40.0%
GGTTGGAAAGTTTCATACAC+AGG - chr3_1:6034669-6034688 None:intergenic 40.0%
ACAAAAAATACGCACCAGGA+AGG - chr3_1:6034740-6034759 None:intergenic 40.0%
TGTACTCCTCCACATTAGAA+AGG + chr3_1:6034855-6034874 Msa0346110:intron 40.0%
TTGGATCCTTTCTAATGTGG+AGG - chr3_1:6034864-6034883 None:intergenic 40.0%
! CTTTTCTCTCATGTTCTGCA+AGG + chr3_1:6035261-6035280 Msa0346110:intron 40.0%
TCCAGAAATCCTTAGTGTTC+GGG - chr3_1:6035328-6035347 None:intergenic 40.0%
CTCCAGAAATCCTTAGTGTT+CGG - chr3_1:6035329-6035348 None:intergenic 40.0%
! CACTAAGGATTTCTGGAGAA+AGG + chr3_1:6035331-6035350 Msa0346110:CDS 40.0%
TCCTGTTCCAGAAAATTGTG+AGG + chr3_1:6035398-6035417 Msa0346110:CDS 40.0%
!!! ACCTCACAATTTTCTGGAAC+AGG - chr3_1:6035402-6035421 None:intergenic 40.0%
TGTTCCAGAAAATTGTGAGG+TGG + chr3_1:6035401-6035420 Msa0346110:CDS 40.0%
! TTCTCCACCTCACAATTTTC+TGG - chr3_1:6035408-6035427 None:intergenic 40.0%
!! GAAGGAATATTGGTTCCCTT+GGG - chr3_1:6035546-6035565 None:intergenic 40.0%
!! TGAAGGAATATTGGTTCCCT+TGG - chr3_1:6035547-6035566 None:intergenic 40.0%
!! TGGAGGCTTTGAAGGAATAT+TGG - chr3_1:6035556-6035575 None:intergenic 40.0%
TCTTCTAGTACTCTAGTTGG+AGG - chr3_1:6035573-6035592 None:intergenic 40.0%
GGTTCTTCTAGTACTCTAGT+TGG - chr3_1:6035576-6035595 None:intergenic 40.0%
TTTCCCTTAGGACTTTGACT+TGG - chr3_1:6035702-6035721 None:intergenic 40.0%
GGCAAGAAAAGAGTCATCAA+AGG + chr3_1:6036001-6036020 Msa0346110:CDS 40.0%
GAAGGGAATCTTCAAAGAAG+TGG + chr3_1:6036121-6036140 Msa0346110:CDS 40.0%
GTTGTCAAGAGAATAGGAGA+AGG + chr3_1:6036161-6036180 Msa0346110:CDS 40.0%
GCACCCATATTTGCAACTAA+GGG - chr3_1:6036209-6036228 None:intergenic 40.0%
AGCACCCATATTTGCAACTA+AGG - chr3_1:6036210-6036229 None:intergenic 40.0%
!! TGGGGAACGTTTTGTTCTAG+AGG - chr3_1:6034643-6034662 None:intergenic 45.0%
!! CCTTCAATTCTGACTTGGGT+TGG - chr3_1:6034686-6034705 None:intergenic 45.0%
CCAACCCAAGTCAGAATTGA+AGG + chr3_1:6034683-6034702 Msa0346110:CDS 45.0%
TTTCTTCACGTTCACCTTCC+TGG + chr3_1:6034723-6034742 Msa0346110:CDS 45.0%
TCCCGAACACTAAGGATTTC+TGG + chr3_1:6035324-6035343 Msa0346110:CDS 45.0%
TCTGGAGAAAGGCAACTACT+TGG + chr3_1:6035342-6035361 Msa0346110:CDS 45.0%
TGTGAGGTGGAGAAACTTCA+AGG + chr3_1:6035414-6035433 Msa0346110:CDS 45.0%
! ACTCTAGTTGGAGGCTTTGA+AGG - chr3_1:6035564-6035583 None:intergenic 45.0%
GGCTCCAAAAGATGCAAAGA+AGG + chr3_1:6036037-6036056 Msa0346110:CDS 45.0%
!! TGGGGAACGTTTTGTTCTAG+AGG - chr3_1:6034643-6034662 None:intergenic 45.0%
!! CCTTCAATTCTGACTTGGGT+TGG - chr3_1:6034686-6034705 None:intergenic 45.0%
CCAACCCAAGTCAGAATTGA+AGG + chr3_1:6034683-6034702 Msa0346110:CDS 45.0%
TTTCTTCACGTTCACCTTCC+TGG + chr3_1:6034723-6034742 Msa0346110:CDS 45.0%
TCCCGAACACTAAGGATTTC+TGG + chr3_1:6035324-6035343 Msa0346110:CDS 45.0%
TCTGGAGAAAGGCAACTACT+TGG + chr3_1:6035342-6035361 Msa0346110:CDS 45.0%
TGTGAGGTGGAGAAACTTCA+AGG + chr3_1:6035414-6035433 Msa0346110:CDS 45.0%
! ACTCTAGTTGGAGGCTTTGA+AGG - chr3_1:6035564-6035583 None:intergenic 45.0%
GGCTCCAAAAGATGCAAAGA+AGG + chr3_1:6036037-6036056 Msa0346110:CDS 45.0%
!!! ATTTTACTTATATTATTATT+AGG - chr3_1:6034789-6034808 None:intergenic 5.0%
!!! ATTTTACTTATATTATTATT+AGG - chr3_1:6034789-6034808 None:intergenic 5.0%
CCAAGTCCTAGAACACCCAA+GGG + chr3_1:6035528-6035547 Msa0346110:CDS 50.0%
TTGGTTCCCTTGGGTGTTCT+AGG - chr3_1:6035537-6035556 None:intergenic 50.0%
CCAAGTCCTAGAACACCCAA+GGG + chr3_1:6035528-6035547 Msa0346110:CDS 50.0%
TTGGTTCCCTTGGGTGTTCT+AGG - chr3_1:6035537-6035556 None:intergenic 50.0%
TGGGACTCTCCCGAACACTA+AGG + chr3_1:6035316-6035335 Msa0346110:CDS 55.0%
GCCAAGTCCTAGAACACCCA+AGG + chr3_1:6035527-6035546 Msa0346110:CDS 55.0%
!! CCCTTGGGTGTTCTAGGACT+TGG - chr3_1:6035531-6035550 None:intergenic 55.0%
TGGGACTCTCCCGAACACTA+AGG + chr3_1:6035316-6035335 Msa0346110:CDS 55.0%
GCCAAGTCCTAGAACACCCA+AGG + chr3_1:6035527-6035546 Msa0346110:CDS 55.0%
!! CCCTTGGGTGTTCTAGGACT+TGG - chr3_1:6035531-6035550 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 6034547 6036459 6034547 ID=Msa0346110;Name=Msa0346110
chr3_1 mRNA 6034547 6036459 6034547 ID=Msa0346110-mRNA-1;Parent=Msa0346110;Name=Msa0346110-mRNA-1;_AED=0.02;_eAED=0.02;_QI=68|1|1|1|1|1|2|161|381
chr3_1 exon 6034547 6034744 6034547 ID=Msa0346110-mRNA-1:exon:1717;Parent=Msa0346110-mRNA-1
chr3_1 exon 6035283 6036459 6035283 ID=Msa0346110-mRNA-1:exon:1718;Parent=Msa0346110-mRNA-1
chr3_1 five_prime_UTR 6034547 6034614 6034547 ID=Msa0346110-mRNA-1:five_prime_utr;Parent=Msa0346110-mRNA-1
chr3_1 CDS 6034615 6034744 6034615 ID=Msa0346110-mRNA-1:cds;Parent=Msa0346110-mRNA-1
chr3_1 CDS 6035283 6036298 6035283 ID=Msa0346110-mRNA-1:cds;Parent=Msa0346110-mRNA-1
chr3_1 three_prime_UTR 6036299 6036459 6036299 ID=Msa0346110-mRNA-1:three_prime_utr;Parent=Msa0346110-mRNA-1
Gene Sequence

>Msa0346110

ATGGCTATGAGATCAAGAACACCTACCTCTAGAACAAAACGTTCCCCAAATCCTGTGTATGAAACTTTCCAACCCAAGTCAGAATTGAAGGAAAATGAGGAAGCATTCTTTCTTCACGTTCACCTTCCTGGTTTTATAAAGGAGAGGATAAATATCAAATTTGTGGGACTCTCCCGAACACTAAGGATTTCTGGAGAAAGGCAACTACTTGGTAGCAACAAATGGAGTCTATTTGACCAATCATATCCTGTTCCAGAAAATTGTGAGGTGGAGAAACTTCAAGGCAAGTTTGAAAATGGAACTCTCATTGTTGCAATGCCAAAAAAATTCCCATCACTTAAATCACAAGTTGAAACAACCAAAGAAAAAATTGTGCCAAGTCCTAGAACACCCAAGGGAACCAATATTCCTTCAAAGCCTCCAACTAGAGTACTAGAAGAACCTATAATTAGAGACAAGAAGGTTCCTTCATCTCCAAGTAATATTGTGACAGGGTTAAATGACTTGAACAAGTTGAAGGAACAAAAAGGTACCCAAGATACTTTTCCAAGTCAAAGTCCTAAGGGAAAATTGAAAGCTTTAGAACCCTTTGTAGAATCTAGACCTCAAAATGTTTATGAGGAAAATAATGTGCCAAAAGCAAAATCCCAAGCAATTGATGATGAAAAAATTCAAGAGGAAAATAGGAAAAAGTCCATATTAGAAGCAATTAAAAAACAATTGCACGAGGACGAAAAGAAAAGTGTCACAAATAAGAAAATTGAAGAAGATAAGAAAACTTATGAGTCACGCAAGCTTGATCAACAATACGTTGATCACAATACGTTTTTAGAAGGAAAAGAAATAAAGGCAAGAAAAGAGTCATCAAAGGGAGAAGAATATTTGGCTCCAAAAGATGCAAAGAAGGAAAAGGATACTACAATTGACAAAGCAAAGAGGTATAAGAAGGATGAAAAGTATACAAAAGAGAAGGGAATCTTCAAAGAAGTGGCAGCTTCAGCTTCTCAAGTTGTCAAGAGAATAGGAGAAGGAAAATTGAATGAACAAGAAAAGCCCTTAGTTGCAAATATGGGTGCTGCAATTCTAGTAATTGTTGCACTTGGAGCTTATGCTACCTATAAGTTTACCTCTTCTAGCAAAAACTAA
Protein sequence

>Msa0346110

MAMRSRTPTSRTKRSPNPVYETFQPKSELKENEEAFFLHVHLPGFIKERINIKFVGLSRTLRISGERQLLGSNKWSLFDQSYPVPENCEVEKLQGKFENGTLIVAMPKKFPSLKSQVETTKEKIVPSPRTPKGTNIPSKPPTRVLEEPIIRDKKVPSSPSNIVTGLNDLNKLKEQKGTQDTFPSQSPKGKLKALEPFVESRPQNVYEENNVPKAKSQAIDDEKIQEENRKKSILEAIKKQLHEDEKKSVTNKKIEEDKKTYESRKLDQQYVDHNTFLEGKEIKARKESSKGEEYLAPKDAKKEKDTTIDKAKRYKKDEKYTKEKGIFKEVAASASQVVKRIGEGKLNEQEKPLVANMGAAILVIVALGAYATYKFTSSSKN*