Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0191020 | XP_003593425.1 | 97.030 | 202 | 6 | 0 | 1 | 202 | 1 | 202 | 1.04e-142 | 405 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0191020 | sp|Q8VZ13|Y1816_ARATH | 35.758 | 165 | 103 | 2 | 41 | 202 | 57 | 221 | 3.53e-33 | 121 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0191020 | G7IK66 | 97.030 | 202 | 6 | 0 | 1 | 202 | 1 | 202 | 4.97e-143 | 405 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0005890 | Msa0191020 | 0.804345 | 2.258559e-49 | -8.615850e-47 |
| Msa0034810 | Msa0191020 | 0.813290 | 2.758732e-51 | -8.615850e-47 |
| Msa0071860 | Msa0191020 | 0.808177 | 3.520427e-50 | -8.615850e-47 |
| Msa0071880 | Msa0191020 | 0.822709 | 2.049207e-53 | -8.615850e-47 |
| Msa0081520 | Msa0191020 | 0.805403 | 1.357349e-49 | -8.615850e-47 |
| Msa0102870 | Msa0191020 | 0.804343 | 2.260741e-49 | -8.615850e-47 |
| Msa0129940 | Msa0191020 | 0.816423 | 5.572082e-52 | -8.615850e-47 |
| Msa0164400 | Msa0191020 | 0.810968 | 8.852792e-51 | -8.615850e-47 |
| Msa0174450 | Msa0191020 | 0.805048 | 1.610832e-49 | -8.615850e-47 |
| Msa0175730 | Msa0191020 | 0.836703 | 8.009624e-57 | -8.615850e-47 |
| Msa0186920 | Msa0191020 | 0.855833 | 4.837913e-62 | -8.615850e-47 |
| Msa0191020 | Msa0194820 | 0.816267 | 6.040193e-52 | -8.615850e-47 |
| Msa0191020 | Msa0194840 | 0.816247 | 6.103262e-52 | -8.615850e-47 |
| Msa0191020 | Msa0211030 | 0.820927 | 5.294732e-53 | -8.615850e-47 |
| Msa0191020 | Msa0225100 | 0.826341 | 2.860431e-54 | -8.615850e-47 |
| Msa0191020 | Msa0225220 | 0.809365 | 1.961443e-50 | -8.615850e-47 |
| Msa0191020 | Msa0225860 | 0.824966 | 6.060257e-54 | -8.615850e-47 |
| Msa0191020 | Msa0233320 | 0.997895 | 1.775019e-251 | -8.615850e-47 |
| Msa0191020 | Msa0243600 | 0.815156 | 1.068020e-51 | -8.615850e-47 |
| Msa0191020 | Msa0245280 | 0.804056 | 2.594361e-49 | -8.615850e-47 |
| Msa0191020 | Msa0266180 | 0.824722 | 6.920527e-54 | -8.615850e-47 |
| Msa0191020 | Msa0282220 | 0.801231 | 9.924765e-49 | -8.615850e-47 |
| Msa0191020 | Msa0287120 | 0.819627 | 1.051875e-52 | -8.615850e-47 |
| Msa0191020 | Msa0287740 | 0.830677 | 2.566204e-55 | -8.615850e-47 |
| Msa0191020 | Msa0287780 | 0.819935 | 8.942147e-53 | -8.615850e-47 |
| Msa0191020 | Msa0298470 | 0.816142 | 6.440971e-52 | -8.615850e-47 |
| Msa0191020 | Msa0300270 | 0.806776 | 6.979628e-50 | -8.615850e-47 |
| Msa0191020 | Msa0321620 | 0.808467 | 3.053807e-50 | -8.615850e-47 |
| Msa0191020 | Msa0337700 | 0.802449 | 5.580295e-49 | -8.615850e-47 |
| Msa0191020 | Msa0342010 | 0.809753 | 1.618996e-50 | -8.615850e-47 |
| Msa0191020 | Msa0346110 | 0.800762 | 1.237768e-48 | -8.615850e-47 |
| Msa0191020 | Msa0359020 | 0.809513 | 1.823149e-50 | -8.615850e-47 |
| Msa0191020 | Msa0367870 | 0.802715 | 4.919806e-49 | -8.615850e-47 |
| Msa0191020 | Msa0371170 | 0.841748 | 3.935980e-58 | -8.615850e-47 |
| Msa0191020 | Msa0376630 | 0.800498 | 1.401049e-48 | -8.615850e-47 |
| Msa0191020 | Msa0378720 | 0.808412 | 3.136580e-50 | -8.615850e-47 |
| Msa0191020 | Msa0388770 | 0.809975 | 1.450328e-50 | -8.615850e-47 |
| Msa0191020 | Msa0393150 | 0.822323 | 2.519302e-53 | -8.615850e-47 |
| Msa0191020 | Msa0405680 | 0.808694 | 2.731294e-50 | -8.615850e-47 |
| Msa0191020 | Msa0415610 | 0.815156 | 1.067917e-51 | -8.615850e-47 |
| Msa0191020 | Msa0418970 | 0.817891 | 2.607017e-52 | -8.615850e-47 |
| Msa0191020 | Msa0421890 | 0.804716 | 1.889750e-49 | -8.615850e-47 |
| Msa0191020 | Msa0425170 | 0.824613 | 7.340400e-54 | -8.615850e-47 |
| Msa0191020 | Msa0460630 | 0.812124 | 4.964439e-51 | -8.615850e-47 |
| Msa0191020 | Msa0465400 | 0.830921 | 2.236076e-55 | -8.615850e-47 |
| Msa0191020 | Msa0467770 | 0.801244 | 9.864136e-49 | -8.615850e-47 |
| Msa0191020 | Msa0510480 | 0.811630 | 6.359672e-51 | -8.615850e-47 |
| Msa0191020 | Msa0528570 | 0.819469 | 1.142581e-52 | -8.615850e-47 |
| Msa0191020 | Msa0531180 | 0.816728 | 4.761479e-52 | -8.615850e-47 |
| Msa0191020 | Msa0557460 | 0.805790 | 1.125942e-49 | -8.615850e-47 |
| Msa0191020 | Msa0562620 | 0.822116 | 2.812829e-53 | -8.615850e-47 |
| Msa0191020 | Msa0565660 | 0.819186 | 1.325296e-52 | -8.615850e-47 |
| Msa0191020 | Msa0571130 | 0.803510 | 3.367845e-49 | -8.615850e-47 |
| Msa0191020 | Msa0580370 | 0.817129 | 3.869675e-52 | -8.615850e-47 |
| Msa0191020 | Msa0583360 | -0.804813 | 1.804224e-49 | -8.615850e-47 |
| Msa0191020 | Msa0600930 | 0.806817 | 6.843085e-50 | -8.615850e-47 |
| Msa0191020 | Msa0616510 | 0.818834 | 1.593424e-52 | -8.615850e-47 |
| Msa0191020 | Msa0626450 | 0.813018 | 3.164507e-51 | -8.615850e-47 |
| Msa0191020 | Msa0626490 | 0.806649 | 7.423057e-50 | -8.615850e-47 |
| Msa0191020 | Msa0664600 | 0.807278 | 5.465413e-50 | -8.615850e-47 |
| Msa0191020 | Msa0699840 | 0.823428 | 1.392665e-53 | -8.615850e-47 |
| Msa0191020 | Msa0701700 | 0.815086 | 1.107152e-51 | -8.615850e-47 |
| Msa0191020 | Msa0741220 | 0.848292 | 6.724441e-60 | -8.615850e-47 |
| Msa0191020 | Msa0741650 | 0.800855 | 1.184921e-48 | -8.615850e-47 |
| Msa0191020 | Msa0780270 | 0.815790 | 7.717022e-52 | -8.615850e-47 |
| Msa0191020 | Msa0795870 | 0.815652 | 8.284534e-52 | -8.615850e-47 |
| Msa0191020 | Msa0796160 | 0.813774 | 2.159006e-51 | -8.615850e-47 |
| Msa0191020 | Msa0823820 | 0.807839 | 4.154885e-50 | -8.615850e-47 |
| Msa0191020 | Msa0829300 | 0.830831 | 2.352524e-55 | -8.615850e-47 |
| Msa0191020 | Msa0829330 | 0.817698 | 2.881331e-52 | -8.615850e-47 |
| Msa0191020 | Msa0833650 | 0.816221 | 6.184601e-52 | -8.615850e-47 |
| Msa0191020 | Msa0833760 | 0.804786 | 1.827456e-49 | -8.615850e-47 |
| Msa0191020 | Msa0863010 | 0.807150 | 5.816503e-50 | -8.615850e-47 |
| Msa0191020 | Msa0896440 | 0.819087 | 1.396132e-52 | -8.615850e-47 |
| Msa0191020 | Msa0905240 | 0.802371 | 5.792067e-49 | -8.615850e-47 |
| Msa0191020 | Msa0922980 | 0.804264 | 2.348724e-49 | -8.615850e-47 |
| Msa0191020 | Msa0923030 | 0.809149 | 2.181978e-50 | -8.615850e-47 |
| Msa0191020 | Msa0965290 | 0.807859 | 4.114837e-50 | -8.615850e-47 |
| Msa0191020 | Msa0967870 | 0.818496 | 1.901975e-52 | -8.615850e-47 |
| Msa0191020 | Msa0996330 | 0.805740 | 1.153631e-49 | -8.615850e-47 |
| Msa0191020 | Msa1020630 | 0.808196 | 3.488247e-50 | -8.615850e-47 |
| Msa0191020 | Msa1031930 | 0.800432 | 1.445092e-48 | -8.615850e-47 |
| Msa0191020 | Msa1032780 | 0.811023 | 8.614533e-51 | -8.615850e-47 |
| Msa0191020 | Msa1106510 | 0.814805 | 1.277572e-51 | -8.615850e-47 |
| Msa0191020 | Msa1113800 | 0.826118 | 3.232239e-54 | -8.615850e-47 |
| Msa0191020 | Msa1132700 | 0.806786 | 6.944125e-50 | -8.615850e-47 |
| Msa0191020 | Msa1132840 | 0.819411 | 1.177834e-52 | -8.615850e-47 |
| Msa0191020 | Msa1155170 | -0.821837 | 3.264501e-53 | -8.615850e-47 |
| Msa0191020 | Msa1160500 | 0.828664 | 7.929118e-55 | -8.615850e-47 |
| Msa0191020 | Msa1161810 | 0.808577 | 2.893034e-50 | -8.615850e-47 |
| Msa0191020 | Msa1225860 | -0.811442 | 6.985413e-51 | -8.615850e-47 |
| Msa0191020 | Msa1226840 | 0.810021 | 1.417472e-50 | -8.615850e-47 |
| Msa0191020 | Msa1242370 | 0.806813 | 6.856318e-50 | -8.615850e-47 |
| Msa0191020 | Msa1263490 | 0.801556 | 8.516035e-49 | -8.615850e-47 |
| Msa0191020 | Msa1265650 | 0.800779 | 1.228023e-48 | -8.615850e-47 |
| Msa0191020 | Msa1341890 | 0.825867 | 3.709307e-54 | -8.615850e-47 |
| Msa0191020 | Msa1414640 | 0.802010 | 6.870753e-49 | -8.615850e-47 |
| Msa0191020 | Msa1415540 | 0.809179 | 2.150549e-50 | -8.615850e-47 |
| Msa0191020 | Msa1433810 | 0.851318 | 9.594348e-61 | -8.615850e-47 |
| Msa0191020 | Msa1440960 | 0.802599 | 5.196129e-49 | -8.615850e-47 |
| Msa0191020 | Msa1450100 | 0.809407 | 1.921828e-50 | -8.615850e-47 |
| Msa0191020 | Msa1463900 | 0.806977 | 6.329452e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0191020 | MtrunA17_Chr2g0280851 | 97.030 | 202 | 6 | 0 | 1 | 202 | 1 | 202 | 9.58e-147 | 405 |
| Msa0191020 | MtrunA17_Chr7g0267281 | 30.952 | 210 | 131 | 7 | 4 | 202 | 20 | 226 | 6.45e-25 | 97.1 |
| Msa0191020 | MtrunA17_Chr3g0109121 | 34.965 | 143 | 86 | 3 | 43 | 181 | 88 | 227 | 2.61e-23 | 93.6 |
| Msa0191020 | MtrunA17_Chr1g0177471 | 32.867 | 143 | 95 | 1 | 40 | 181 | 73 | 215 | 5.43e-23 | 92.8 |
| Msa0191020 | MtrunA17_Chr7g0267321 | 32.022 | 178 | 115 | 4 | 14 | 188 | 162 | 336 | 7.26e-23 | 94.4 |
| Msa0191020 | MtrunA17_Chr4g0067041 | 31.915 | 141 | 95 | 1 | 34 | 173 | 64 | 204 | 9.71e-23 | 92.0 |
| Msa0191020 | MtrunA17_Chr5g0440571 | 31.515 | 165 | 108 | 3 | 20 | 180 | 67 | 230 | 1.21e-21 | 89.4 |
| Msa0191020 | MtrunA17_Chr7g0276371 | 28.070 | 171 | 119 | 3 | 22 | 190 | 44 | 212 | 3.72e-21 | 87.4 |
| Msa0191020 | MtrunA17_Chr7g0267181 | 33.690 | 187 | 113 | 4 | 4 | 181 | 18 | 202 | 1.83e-20 | 85.5 |
| Msa0191020 | MtrunA17_Chr7g0267371 | 29.834 | 181 | 118 | 4 | 11 | 183 | 3 | 182 | 1.94e-20 | 84.7 |
| Msa0191020 | MtrunA17_Chr3g0118371 | 28.846 | 156 | 109 | 2 | 27 | 180 | 73 | 228 | 1.62e-19 | 83.6 |
| Msa0191020 | MtrunA17_Chr7g0267361 | 31.361 | 169 | 110 | 4 | 18 | 183 | 68 | 233 | 2.26e-19 | 83.2 |
| Msa0191020 | MtrunA17_Chr5g0433201 | 33.333 | 153 | 101 | 1 | 41 | 192 | 97 | 249 | 2.26e-19 | 83.2 |
| Msa0191020 | MtrunA17_Chr2g0321801 | 26.344 | 186 | 122 | 4 | 20 | 192 | 15 | 198 | 1.05e-18 | 80.5 |
| Msa0191020 | MtrunA17_Chr2g0281401 | 28.022 | 182 | 123 | 5 | 23 | 199 | 80 | 258 | 2.19e-18 | 80.5 |
| Msa0191020 | MtrunA17_Chr7g0267291 | 30.319 | 188 | 121 | 5 | 2 | 180 | 13 | 199 | 9.19e-18 | 79.7 |
| Msa0191020 | MtrunA17_Chr1g0188801 | 31.902 | 163 | 107 | 3 | 43 | 202 | 87 | 248 | 5.48e-17 | 76.6 |
| Msa0191020 | MtrunA17_Chr6g0478671 | 33.663 | 101 | 65 | 2 | 22 | 122 | 9 | 107 | 1.04e-16 | 75.1 |
| Msa0191020 | MtrunA17_Chr6g0479081 | 29.747 | 158 | 106 | 3 | 28 | 180 | 15 | 172 | 1.36e-16 | 74.7 |
| Msa0191020 | MtrunA17_Chr1g0177571 | 29.240 | 171 | 114 | 4 | 14 | 180 | 10 | 177 | 1.46e-16 | 74.7 |
| Msa0191020 | MtrunA17_Chr4g0025681 | 25.773 | 194 | 138 | 4 | 1 | 191 | 3 | 193 | 1.76e-16 | 74.7 |
| Msa0191020 | MtrunA17_Chr3g0127951 | 27.143 | 140 | 100 | 2 | 47 | 185 | 49 | 187 | 4.87e-16 | 73.2 |
| Msa0191020 | MtrunA17_Chr2g0321831 | 26.500 | 200 | 139 | 5 | 3 | 195 | 4 | 202 | 5.17e-16 | 73.2 |
| Msa0191020 | MtrunA17_Chr7g0267301 | 28.261 | 184 | 123 | 4 | 5 | 180 | 21 | 203 | 4.41e-15 | 71.2 |
| Msa0191020 | MtrunA17_Chr6g0479071 | 31.579 | 95 | 65 | 0 | 28 | 122 | 15 | 109 | 5.93e-15 | 70.5 |
| Msa0191020 | MtrunA17_Chr3g0118391 | 26.429 | 140 | 101 | 2 | 43 | 181 | 45 | 183 | 4.54e-14 | 68.2 |
| Msa0191020 | MtrunA17_Chr2g0330241 | 24.000 | 175 | 125 | 3 | 36 | 202 | 77 | 251 | 7.77e-13 | 65.5 |
| Msa0191020 | MtrunA17_Chr3g0115651 | 25.786 | 159 | 110 | 2 | 23 | 180 | 70 | 221 | 1.01e-12 | 65.1 |
| Msa0191020 | MtrunA17_Chr7g0267231 | 25.000 | 160 | 114 | 3 | 13 | 167 | 31 | 189 | 2.82e-12 | 63.5 |
| Msa0191020 | MtrunA17_Chr4g0025861 | 23.226 | 155 | 109 | 3 | 47 | 192 | 44 | 197 | 2.47e-11 | 60.8 |
| Msa0191020 | MtrunA17_Chr3g0118381 | 31.481 | 108 | 72 | 2 | 75 | 180 | 14 | 121 | 2.82e-11 | 59.3 |
| Msa0191020 | MtrunA17_Chr5g0447101 | 21.739 | 161 | 117 | 3 | 11 | 168 | 50 | 204 | 9.07e-11 | 59.3 |
| Msa0191020 | MtrunA17_Chr6g0478661 | 31.313 | 99 | 67 | 1 | 24 | 122 | 7 | 104 | 9.87e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0191020 | AT5G22870.1 | 42.647 | 204 | 113 | 1 | 3 | 202 | 4 | 207 | 4.53e-60 | 187 |
| Msa0191020 | AT1G08160.1 | 35.758 | 165 | 103 | 2 | 41 | 202 | 57 | 221 | 3.59e-34 | 121 |
| Msa0191020 | AT5G06320.1 | 33.511 | 188 | 120 | 3 | 20 | 202 | 44 | 231 | 9.27e-33 | 118 |
| Msa0191020 | AT2G35980.1 | 35.542 | 166 | 105 | 2 | 17 | 180 | 34 | 199 | 8.13e-32 | 115 |
| Msa0191020 | AT3G11650.1 | 30.208 | 192 | 128 | 2 | 17 | 202 | 48 | 239 | 9.69e-31 | 113 |
| Msa0191020 | AT3G52470.1 | 36.145 | 166 | 99 | 4 | 19 | 180 | 14 | 176 | 9.10e-26 | 99.4 |
| Msa0191020 | AT2G35460.1 | 29.282 | 181 | 125 | 3 | 17 | 195 | 47 | 226 | 4.07e-25 | 98.2 |
| Msa0191020 | AT4G09590.1 | 31.579 | 171 | 114 | 2 | 24 | 192 | 22 | 191 | 2.55e-23 | 92.8 |
| Msa0191020 | AT3G11660.1 | 30.233 | 172 | 116 | 3 | 20 | 188 | 14 | 184 | 6.52e-23 | 92.0 |
| Msa0191020 | AT2G27080.1 | 29.221 | 154 | 108 | 1 | 28 | 180 | 82 | 235 | 2.82e-22 | 91.3 |
| Msa0191020 | AT2G27080.2 | 29.221 | 154 | 108 | 1 | 28 | 180 | 82 | 235 | 2.82e-22 | 91.3 |
| Msa0191020 | AT3G44220.1 | 27.848 | 158 | 113 | 1 | 24 | 180 | 20 | 177 | 1.36e-21 | 88.2 |
| Msa0191020 | AT5G53730.1 | 30.612 | 147 | 99 | 3 | 37 | 180 | 40 | 186 | 6.66e-21 | 86.7 |
| Msa0191020 | AT2G35970.1 | 30.994 | 171 | 115 | 2 | 24 | 192 | 22 | 191 | 1.11e-20 | 85.9 |
| Msa0191020 | AT1G65690.1 | 30.000 | 160 | 111 | 1 | 23 | 181 | 68 | 227 | 1.95e-20 | 86.3 |
| Msa0191020 | AT2G35960.1 | 34.932 | 146 | 88 | 4 | 39 | 180 | 36 | 178 | 4.86e-20 | 84.3 |
| Msa0191020 | AT3G52460.1 | 28.796 | 191 | 127 | 3 | 5 | 187 | 90 | 279 | 4.81e-19 | 83.2 |
| Msa0191020 | AT5G22200.1 | 29.114 | 158 | 111 | 1 | 24 | 180 | 24 | 181 | 1.37e-18 | 80.5 |
| Msa0191020 | AT5G06330.1 | 30.719 | 153 | 102 | 3 | 43 | 192 | 39 | 190 | 3.20e-18 | 79.3 |
| Msa0191020 | AT1G61760.1 | 27.835 | 194 | 120 | 5 | 5 | 180 | 9 | 200 | 3.55e-18 | 79.7 |
| Msa0191020 | AT4G01410.1 | 28.125 | 160 | 114 | 1 | 22 | 180 | 42 | 201 | 5.38e-18 | 79.3 |
| Msa0191020 | AT4G05220.1 | 26.471 | 170 | 121 | 3 | 17 | 184 | 39 | 206 | 1.26e-17 | 78.2 |
| Msa0191020 | AT5G21130.1 | 26.230 | 183 | 134 | 1 | 21 | 202 | 96 | 278 | 1.10e-15 | 73.9 |
| Msa0191020 | AT5G36970.1 | 30.612 | 147 | 101 | 1 | 36 | 181 | 77 | 223 | 3.62e-15 | 72.0 |
| Msa0191020 | AT1G54540.1 | 30.405 | 148 | 99 | 3 | 43 | 188 | 76 | 221 | 4.11e-14 | 68.9 |
| Msa0191020 | AT2G27260.1 | 27.072 | 181 | 123 | 4 | 7 | 186 | 51 | 223 | 1.48e-12 | 64.7 |
Find 31 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATTGACACTTTGGAATT+TGG | 0.133624 | 2_1:+6554641 | None:intergenic |
| CAGAGCAAGGATAAGGTTTA+AGG | 0.222903 | 2_1:-6554417 | Msa0191020:CDS |
| TATTAAGGCTAAAATTATTA+TGG | 0.226061 | 2_1:+6554817 | None:intergenic |
| CTTAAGCTTGAGAGATCTTC+TGG | 0.228941 | 2_1:-6554464 | Msa0191020:CDS |
| CTCTCAAGCTTAAGATCTTT+TGG | 0.241960 | 2_1:+6554473 | None:intergenic |
| AAACGAAACACCGTACTCTA+AGG | 0.304258 | 2_1:-6554382 | Msa0191020:CDS |
| TAAGCCAACAATGATTATTA+AGG | 0.333073 | 2_1:+6554802 | None:intergenic |
| TTACATTTCAACATCACAAT+AGG | 0.375265 | 2_1:+6554304 | None:intergenic |
| TTAGCCTTAATAATCATTGT+TGG | 0.424677 | 2_1:-6554806 | Msa0191020:CDS |
| GTTGAACTGCATTTGTAGCT+AGG | 0.443513 | 2_1:+6554576 | None:intergenic |
| TGTTCATCAGAGCAAGGATA+AGG | 0.446104 | 2_1:-6554424 | Msa0191020:CDS |
| ATCATAGTATATTGACACTT+TGG | 0.456538 | 2_1:+6554634 | None:intergenic |
| GCTTGAGGATTTGATTGTGT+TGG | 0.466829 | 2_1:+6554878 | None:intergenic |
| TCATAAGTTGGCTAGTTGTA+AGG | 0.471372 | 2_1:-6554772 | Msa0191020:CDS |
| TGAGGATTTGATTGTGTTGG+AGG | 0.474694 | 2_1:+6554881 | None:intergenic |
| CATCAGTTAAATTGAAGTTG+TGG | 0.491299 | 2_1:+6554711 | None:intergenic |
| GCTTAGCTGTACTCATAAGT+TGG | 0.503619 | 2_1:-6554784 | Msa0191020:CDS |
| GCAAGGATAAGGTTTAAGGT+TGG | 0.511168 | 2_1:-6554413 | Msa0191020:CDS |
| TTGAACTGCATTTGTAGCTA+GGG | 0.511309 | 2_1:+6554577 | None:intergenic |
| TTAAGCTTGAGAGATCTTCT+GGG | 0.516407 | 2_1:-6554463 | Msa0191020:CDS |
| CTATGATTCTATTGAAGTGT+CGG | 0.534724 | 2_1:-6554618 | Msa0191020:CDS |
| GGTATCTTGTTCACCTGTGT+TGG | 0.539217 | 2_1:-6554360 | Msa0191020:CDS |
| ATCCTAGTAACATTCTTATG+TGG | 0.542159 | 2_1:+6554542 | None:intergenic |
| AACCACATAAGAATGTTACT+AGG | 0.554394 | 2_1:-6554544 | Msa0191020:CDS |
| TGAAGTGTCGGTGCGTTACG+AGG | 0.582552 | 2_1:-6554606 | Msa0191020:CDS |
| AAGACTCACCGCTCAAACTG+TGG | 0.584343 | 2_1:-6554513 | Msa0191020:CDS |
| AACGAAACACCGTACTCTAA+GGG | 0.584440 | 2_1:-6554381 | Msa0191020:CDS |
| AACAAGATACCCTTAGAGTA+CGG | 0.596628 | 2_1:+6554372 | None:intergenic |
| TAGATGTGTTCATCAGAGCA+AGG | 0.642918 | 2_1:-6554430 | Msa0191020:CDS |
| TAAGCTTGAGAGATCTTCTG+GGG | 0.656491 | 2_1:-6554462 | Msa0191020:CDS |
| ATACAAAGCCACAGTTTGAG+CGG | 0.660862 | 2_1:+6554505 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTAAGGCTAAAATTATTA+TGG | + | chr2_1:6554380-6554399 | None:intergenic | 15.0% |
| !!! | TTAGCCTTAATAATCATTGT+TGG | - | chr2_1:6554388-6554407 | Msa0191020:CDS | 25.0% |
| ! | TAAGCCAACAATGATTATTA+AGG | + | chr2_1:6554395-6554414 | None:intergenic | 25.0% |
| !!! | ACACTATATTGCAAGTTTTT+TGG | + | chr2_1:6554448-6554467 | None:intergenic | 25.0% |
| !!! | TATATTGACACTTTGGAATT+TGG | + | chr2_1:6554556-6554575 | None:intergenic | 25.0% |
| ! | ATCATAGTATATTGACACTT+TGG | + | chr2_1:6554563-6554582 | None:intergenic | 25.0% |
| ! | TTCTTATGTGGTTGAAAAAA+CGG | + | chr2_1:6554643-6554662 | None:intergenic | 25.0% |
| !!! | TAAAGCTAAGAGTTTTGAAA+GGG | - | chr2_1:6554867-6554886 | Msa0191020:CDS | 25.0% |
| ! | TTACATTTCAACATCACAAT+AGG | + | chr2_1:6554893-6554912 | None:intergenic | 25.0% |
| CATCAGTTAAATTGAAGTTG+TGG | + | chr2_1:6554486-6554505 | None:intergenic | 30.0% | |
| CTATGATTCTATTGAAGTGT+CGG | - | chr2_1:6554576-6554595 | Msa0191020:CDS | 30.0% | |
| AACCACATAAGAATGTTACT+AGG | - | chr2_1:6554650-6554669 | Msa0191020:CDS | 30.0% | |
| ATCCTAGTAACATTCTTATG+TGG | + | chr2_1:6554655-6554674 | None:intergenic | 30.0% | |
| TTAGAAAAATTCACCAACAC+AGG | + | chr2_1:6554850-6554869 | None:intergenic | 30.0% | |
| !!! | CTAAAGCTAAGAGTTTTGAA+AGG | - | chr2_1:6554866-6554885 | Msa0191020:CDS | 30.0% |
| !!! | CAGCATTGAACTTTTTGGTT+TGG | + | chr2_1:6554344-6554363 | None:intergenic | 35.0% |
| ! | TCATAAGTTGGCTAGTTGTA+AGG | - | chr2_1:6554422-6554441 | Msa0191020:CDS | 35.0% |
| TTGAACTGCATTTGTAGCTA+GGG | + | chr2_1:6554620-6554639 | None:intergenic | 35.0% | |
| ! | CTCTCAAGCTTAAGATCTTT+TGG | + | chr2_1:6554724-6554743 | None:intergenic | 35.0% |
| TTAAGCTTGAGAGATCTTCT+GGG | - | chr2_1:6554731-6554750 | Msa0191020:CDS | 35.0% | |
| AACAAGATACCCTTAGAGTA+CGG | + | chr2_1:6554825-6554844 | None:intergenic | 35.0% | |
| TGAGGATTTGATTGTGTTGG+AGG | + | chr2_1:6554316-6554335 | None:intergenic | 40.0% | |
| GCTTGAGGATTTGATTGTGT+TGG | + | chr2_1:6554319-6554338 | None:intergenic | 40.0% | |
| !!! | CTTTTTGGTTTGGTTGCTTG+AGG | + | chr2_1:6554334-6554353 | None:intergenic | 40.0% |
| !! | CGTAGCAGCATTGAACTTTT+TGG | + | chr2_1:6554349-6554368 | None:intergenic | 40.0% |
| GCTTAGCTGTACTCATAAGT+TGG | - | chr2_1:6554410-6554429 | Msa0191020:CDS | 40.0% | |
| GTTGAACTGCATTTGTAGCT+AGG | + | chr2_1:6554621-6554640 | None:intergenic | 40.0% | |
| ATACAAAGCCACAGTTTGAG+CGG | + | chr2_1:6554692-6554711 | None:intergenic | 40.0% | |
| CTTAAGCTTGAGAGATCTTC+TGG | - | chr2_1:6554730-6554749 | Msa0191020:CDS | 40.0% | |
| TAAGCTTGAGAGATCTTCTG+GGG | - | chr2_1:6554732-6554751 | Msa0191020:CDS | 40.0% | |
| TAGATGTGTTCATCAGAGCA+AGG | - | chr2_1:6554764-6554783 | Msa0191020:CDS | 40.0% | |
| TGTTCATCAGAGCAAGGATA+AGG | - | chr2_1:6554770-6554789 | Msa0191020:CDS | 40.0% | |
| CAGAGCAAGGATAAGGTTTA+AGG | - | chr2_1:6554777-6554796 | Msa0191020:CDS | 40.0% | |
| ! | GCAAGGATAAGGTTTAAGGT+TGG | - | chr2_1:6554781-6554800 | Msa0191020:CDS | 40.0% |
| AAACGAAACACCGTACTCTA+AGG | - | chr2_1:6554812-6554831 | Msa0191020:CDS | 40.0% | |
| AACGAAACACCGTACTCTAA+GGG | - | chr2_1:6554813-6554832 | Msa0191020:CDS | 40.0% | |
| GGTATCTTGTTCACCTGTGT+TGG | - | chr2_1:6554834-6554853 | Msa0191020:CDS | 45.0% | |
| AAGACTCACCGCTCAAACTG+TGG | - | chr2_1:6554681-6554700 | Msa0191020:CDS | 50.0% | |
| !! | TGAAGTGTCGGTGCGTTACG+AGG | - | chr2_1:6554588-6554607 | Msa0191020:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 6554304 | 6554912 | 6554304 | ID=Msa0191020;Name=Msa0191020 |
| chr2_1 | mRNA | 6554304 | 6554912 | 6554304 | ID=Msa0191020-mRNA-1;Parent=Msa0191020;Name=Msa0191020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|202 |
| chr2_1 | exon | 6554304 | 6554912 | 6554304 | ID=Msa0191020-mRNA-1:exon:2938;Parent=Msa0191020-mRNA-1 |
| chr2_1 | CDS | 6554304 | 6554912 | 6554304 | ID=Msa0191020-mRNA-1:cds;Parent=Msa0191020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
