AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0349430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0349430 MtrunA17_Chr3g0082861 97.006 167 5 0 1 167 1 167 1.47e-118 332
Msa0349430 MtrunA17_Chr8g0352871 73.939 165 42 1 3 167 8 171 8.00e-84 244
Msa0349430 MtrunA17_Chr7g0256551 67.901 162 50 1 2 163 7 166 1.32e-77 231
Msa0349430 MtrunA17_Chr3g0124861 59.119 159 50 4 3 150 21 175 1.11e-58 182
Msa0349430 MtrunA17_Chr5g0414931 57.971 138 53 1 3 140 15 147 5.88e-53 168
Msa0349430 MtrunA17_Chr4g0059571 52.147 163 69 2 2 164 24 177 2.27e-51 163
Msa0349430 MtrunA17_Chr3g0118211 50.307 163 72 2 1 163 20 173 2.75e-51 162
Msa0349430 MtrunA17_Chr1g0170741 57.971 138 52 1 3 140 7 138 5.92e-51 162
Msa0349430 MtrunA17_Chr3g0128691 55.634 142 46 3 5 141 19 148 3.47e-50 158
Msa0349430 MtrunA17_Chr4g0021731 73.864 88 14 1 3 90 10 88 1.05e-40 132
Msa0349430 MtrunA17_Chr4g0030271 29.927 137 78 2 6 142 91 209 1.39e-14 70.5
Msa0349430 MtrunA17_Chr8g0345901 27.007 137 82 1 6 142 38 156 2.46e-14 69.7
Msa0349430 MtrunA17_Chr1g0181811 27.737 137 81 1 6 142 87 205 5.39e-13 65.9
Msa0349430 MtrunA17_Chr3g0127941 28.467 137 80 2 6 142 58 176 7.88e-13 65.5
Msa0349430 MtrunA17_Chr7g0276361 24.074 162 103 2 1 162 13 154 5.85e-12 62.8
Msa0349430 MtrunA17_Chr7g0275401 29.221 154 85 4 4 154 9 141 8.47e-12 62.0
Msa0349430 MtrunA17_Chr7g0275891 28.777 139 79 3 6 144 11 129 4.54e-11 60.1
Msa0349430 MtrunA17_Chr1g0161431 28.125 160 93 4 2 161 13 150 4.54e-11 60.1
Msa0349430 MtrunA17_Chr1g0149851 28.671 143 81 3 6 148 11 132 5.93e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0349430 AT3G57040.2 69.799 149 42 2 2 148 7 154 4.63e-71 214
Msa0349430 AT3G57040.1 69.799 149 42 2 2 148 7 154 4.63e-71 214
Msa0349430 AT2G41310.1 64.430 149 52 1 2 150 7 154 6.89e-67 203
Msa0349430 AT1G59940.1 56.604 159 55 4 3 160 32 177 1.16e-55 175
Msa0349430 AT1G10470.3 55.556 162 57 5 1 161 17 164 3.03e-54 172
Msa0349430 AT1G10470.1 56.250 160 55 5 3 161 33 178 3.31e-54 172
Msa0349430 AT1G59940.2 60.714 140 46 2 3 142 124 254 7.33e-54 173
Msa0349430 AT3G48100.1 53.988 163 60 4 4 163 25 175 3.43e-53 167
Msa0349430 AT3G56380.1 55.634 142 55 2 3 144 19 152 2.41e-52 164
Msa0349430 AT2G40670.1 53.521 142 60 1 3 144 28 163 2.70e-51 162
Msa0349430 AT5G62920.1 57.447 141 51 2 4 144 25 156 1.19e-50 161
Msa0349430 AT3G56380.2 55.245 143 55 3 3 144 19 153 1.74e-50 159
Msa0349430 AT2G40670.2 53.147 143 60 2 3 144 28 164 2.24e-49 157
Msa0349430 AT1G19050.1 50.000 158 69 2 3 159 23 171 5.57e-49 157
Msa0349430 AT1G74890.1 50.625 160 68 2 3 160 17 167 7.98e-49 157
Msa0349430 AT2G07440.1 62.687 67 24 1 85 150 2 68 3.96e-22 86.7
Msa0349430 AT1G10470.2 52.857 70 27 2 90 158 35 99 3.13e-16 72.8
Msa0349430 AT5G24470.1 28.777 139 79 3 6 144 54 172 4.44e-13 66.6
Msa0349430 AT2G25180.1 28.105 153 88 4 6 157 19 150 4.33e-12 63.5
Msa0349430 AT5G60100.7 28.467 137 80 1 6 142 66 184 5.13e-12 63.2
Msa0349430 AT5G60100.6 28.467 137 80 1 6 142 66 184 5.13e-12 63.2
Msa0349430 AT5G60100.4 28.467 137 80 1 6 142 66 184 5.13e-12 63.2
Msa0349430 AT5G60100.2 28.467 137 80 1 6 142 66 184 6.06e-12 63.2
Msa0349430 AT5G60100.5 28.467 137 80 1 6 142 66 184 1.09e-11 62.4
Msa0349430 AT5G60100.3 28.467 137 80 1 6 142 66 184 1.09e-11 62.4
Msa0349430 AT5G60100.1 28.467 137 80 1 6 142 66 184 1.09e-11 62.4
Msa0349430 AT5G07210.2 30.000 140 76 3 5 144 17 134 1.57e-11 62.0
Msa0349430 AT2G46790.3 26.619 139 84 2 4 142 37 157 1.68e-11 62.0
Msa0349430 AT2G46790.1 26.619 139 84 2 4 142 37 157 2.01e-11 61.6
Msa0349430 AT5G02810.1 26.277 137 83 2 6 142 80 198 3.26e-11 61.2

Find 53 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGCACAGTTTCATATTT+TGG 0.112277 3_1:+13014831 Msa0349430:exon
AAGAATGTTTGAGGATAATA+AGG 0.190228 3_1:-13014765 None:intergenic
AACATTTACTGTCCAAATTA+TGG 0.196728 3_1:+13014813 Msa0349430:exon
TATGAAACTGTGCCATAATT+TGG 0.209887 3_1:-13014825 None:intergenic
TCAGTTACTGTGGTTGATTC+TGG 0.242120 3_1:+13015381 Msa0349430:intron
CTCAAAACCTCTTCATTTCA+TGG 0.264136 3_1:+13014898 Msa0349430:CDS
GGCTTTGAAGTTTCTTGGTT+TGG 0.274386 3_1:+13015410 Msa0349430:CDS
ACTAAGGCTTTGAAGTTTCT+TGG 0.296459 3_1:+13015405 Msa0349430:CDS
GGTTACCTGTTAATCCTTGA+TGG 0.331015 3_1:-13016284 None:intergenic
TGTGGTTGATTCTGGTACTA+AGG 0.342411 3_1:+13015389 Msa0349430:CDS
TCCTAGGAAATCTGACAGTT+TGG 0.350175 3_1:-13014645 None:intergenic
TTAACATCTGATTGTTGAAC+TGG 0.359149 3_1:-13016455 None:intergenic
ATAGGAGTTTACAAACAAAA+TGG 0.363836 3_1:-13016923 None:intergenic
AGATCATAGCCTGTCATTCC+TGG 0.369947 3_1:-13015647 None:intergenic
ATGTTCCATCAAGGATTAAC+AGG 0.384039 3_1:+13016279 Msa0349430:CDS
AGCTCTTCTCACTAATTTGT+TGG 0.387474 3_1:-13016667 None:intergenic
AAAGAAGTTAAGAATGTTTG+AGG 0.402917 3_1:-13014774 None:intergenic
GTCACATATCAGTTTCAAAT+AGG 0.414453 3_1:-13016941 None:intergenic
GTTTCTGCAGCAATAGTTTG+AGG 0.420290 3_1:-13015459 None:intergenic
AGAAGAGCTTGCTGATGAAT+AGG 0.435827 3_1:+13016681 Msa0349430:three_prime_UTR
GGAAGAATCACATCATTTGT+TGG 0.438550 3_1:-13016892 None:intergenic
ATTGGTCCATGGATAACTCT+TGG 0.444241 3_1:-13016850 None:intergenic
TTGGTCCATGGATAACTCTT+GGG 0.449353 3_1:-13016849 None:intergenic
ATAGGTCATTGATTGATAGT+AGG 0.451798 3_1:+13016699 Msa0349430:three_prime_UTR
TACTGTATGCCAGGAATGAC+AGG 0.454246 3_1:+13015638 Msa0349430:CDS
TCCAAACTGTCAGATTTCCT+AGG 0.495654 3_1:+13014644 Msa0349430:five_prime_UTR
TTCTCACTAATTTGTTGGTG+AGG 0.504206 3_1:-13016662 None:intergenic
AGGAGATACAAAATCCTCCT+AGG 0.511110 3_1:-13014661 None:intergenic
TAGGGTGGACATCATATTCA+AGG 0.515357 3_1:-13014681 None:intergenic
TGAATCAGATGCTTAGAAGA+AGG 0.516221 3_1:+13016410 Msa0349430:intron
ATAACTGATTACTGTATGCC+AGG 0.518630 3_1:+13015629 Msa0349430:CDS
ACCTAATCAACAACAAAAGA+AGG 0.519626 3_1:+13016525 Msa0349430:CDS
TTGACAGAATGTTGATTGAA+AGG 0.523765 3_1:+13014872 Msa0349430:CDS
TCCATGGATAACTCTTGGGC+TGG 0.530715 3_1:-13016845 None:intergenic
AAAATTACCATGAAATGAAG+AGG 0.534519 3_1:-13014905 None:intergenic
AGAATGTTTGAGGATAATAA+GGG 0.537250 3_1:-13014764 None:intergenic
ATTCTGCAATGAAATCTGTC+AGG 0.543289 3_1:+13014612 Msa0349430:five_prime_UTR
GATTTCATTGCAGAATCTGC+CGG 0.543870 3_1:-13014605 None:intergenic
TATTGCTGCAGAAACAGATC+AGG 0.546049 3_1:+13015467 Msa0349430:CDS
AAGATAAAGATGAATGAGAG+AGG 0.554093 3_1:-13014718 None:intergenic
TCTTGGTTTGGTTGAAGATG+AGG 0.555035 3_1:+13015422 Msa0349430:CDS
GTTTGAGGATAATAAGGGAG+GGG 0.567314 3_1:-13014759 None:intergenic
TGTTTGAGGATAATAAGGGA+GGG 0.583699 3_1:-13014760 None:intergenic
AAACTGTCAGATTTCCTAGG+AGG 0.585928 3_1:+13014647 Msa0349430:five_prime_UTR
CCTCAGAGAATGTTCCATCA+AGG 0.587746 3_1:+13016270 Msa0349430:CDS
TCTGAGGACATAATCACCAC+TGG 0.603381 3_1:-13016254 None:intergenic
TCCAGCCCAAGAGTTATCCA+TGG 0.607199 3_1:+13016844 Msa0349430:three_prime_UTR
ATGTTTGAGGATAATAAGGG+AGG 0.612323 3_1:-13014761 None:intergenic
TAAGGGAGGGGAAATAGAAG+AGG 0.612847 3_1:-13014747 None:intergenic
AAGGGAGGGGAAATAGAAGA+GGG 0.615574 3_1:-13014746 None:intergenic
GTTTGGTTGAAGATGAGGTG+AGG 0.669904 3_1:+13015427 Msa0349430:CDS
TTCTCTTAAAGACATACCAG+TGG 0.689344 3_1:+13016238 Msa0349430:CDS
CCTTGATGGAACATTCTCTG+AGG 0.703773 3_1:-13016270 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAAAAAATTTAGAAAATA+TGG + chr3_1:13015103-13015122 Msa0349430:intron 10.0%
!! CTTAAAAAATTTAGAAAATA+TGG + chr3_1:13015103-13015122 Msa0349430:intron 10.0%
!!! TATTTGAATTTTGATGTAAA+AGG - chr3_1:13014971-13014990 None:intergenic 15.0%
!!! ATTCAAATAATTTTGTGAAT+TGG + chr3_1:13014982-13015001 Msa0349430:intron 15.0%
!! ACAAAAATAATATGATTATG+TGG + chr3_1:13015158-13015177 Msa0349430:intron 15.0%
!! TATGAATACTATCAAAAAAT+TGG - chr3_1:13016144-13016163 None:intergenic 15.0%
!! TTAATGTCATGATATTATTA+AGG - chr3_1:13016308-13016327 None:intergenic 15.0%
!!! TATTTGAATTTTGATGTAAA+AGG - chr3_1:13014971-13014990 None:intergenic 15.0%
!!! ATTCAAATAATTTTGTGAAT+TGG + chr3_1:13014982-13015001 Msa0349430:intron 15.0%
!! ACAAAAATAATATGATTATG+TGG + chr3_1:13015158-13015177 Msa0349430:intron 15.0%
!! TATGAATACTATCAAAAAAT+TGG - chr3_1:13016144-13016163 None:intergenic 15.0%
!! TTAATGTCATGATATTATTA+AGG - chr3_1:13016308-13016327 None:intergenic 15.0%
!!! TTTTCTCTCATATTTTTTAG+AGG + chr3_1:13014927-13014946 Msa0349430:intron 20.0%
!!! AAATTTTGTAAAGAACAAAC+TGG - chr3_1:13015058-13015077 None:intergenic 20.0%
!!! CAATTTTGAACGAATTTTTT+TGG + chr3_1:13015202-13015221 Msa0349430:intron 20.0%
!! GGTAATATGTAATGTTTATT+TGG + chr3_1:13015223-13015242 Msa0349430:intron 20.0%
!!! ATGTATTAGTCTAATCATTT+AGG - chr3_1:13015505-13015524 None:intergenic 20.0%
!! AATGATTAGACTAATACATA+TGG + chr3_1:13015506-13015525 Msa0349430:intron 20.0%
!! AAAAATCAAGGTAAATTCAT+TGG + chr3_1:13015676-13015695 Msa0349430:intron 20.0%
!!! TATTTGAAAATAGTTTGTGT+TGG + chr3_1:13015700-13015719 Msa0349430:intron 20.0%
!!! AAAACCACAATTTAAAATCT+TGG - chr3_1:13015887-13015906 None:intergenic 20.0%
!! AAAATCAAAAATTTGTGGTT+TGG - chr3_1:13015922-13015941 None:intergenic 20.0%
!!! AGAAAATATATTTGATTTGC+AGG + chr3_1:13016208-13016227 Msa0349430:intron 20.0%
!! AAATTTCGAATTCAAAACTT+TGG + chr3_1:13016336-13016355 Msa0349430:intron 20.0%
!! AAAAATGTAACCTATAAAAG+TGG + chr3_1:13016584-13016603 Msa0349430:three_prime_UTR 20.0%
!!! TTTAATGTAACCACTTTTAT+AGG - chr3_1:13016597-13016616 None:intergenic 20.0%
!!! ATTAAAAATTGATGATGCAT+AGG + chr3_1:13016611-13016630 Msa0349430:three_prime_UTR 20.0%
!!! TTCAATTTTTGTCTCATAAA+TGG + chr3_1:13016745-13016764 Msa0349430:three_prime_UTR 20.0%
!! ACAAACAAAATGGAAAAATT+TGG - chr3_1:13016916-13016935 None:intergenic 20.0%
!!! TTTTCTCTCATATTTTTTAG+AGG + chr3_1:13014927-13014946 Msa0349430:intron 20.0%
!!! AAATTTTGTAAAGAACAAAC+TGG - chr3_1:13015058-13015077 None:intergenic 20.0%
!!! CAATTTTGAACGAATTTTTT+TGG + chr3_1:13015202-13015221 Msa0349430:intron 20.0%
!! GGTAATATGTAATGTTTATT+TGG + chr3_1:13015223-13015242 Msa0349430:intron 20.0%
!!! ATGTATTAGTCTAATCATTT+AGG - chr3_1:13015505-13015524 None:intergenic 20.0%
!! AATGATTAGACTAATACATA+TGG + chr3_1:13015506-13015525 Msa0349430:intron 20.0%
!! AAAAATCAAGGTAAATTCAT+TGG + chr3_1:13015676-13015695 Msa0349430:intron 20.0%
!!! TATTTGAAAATAGTTTGTGT+TGG + chr3_1:13015700-13015719 Msa0349430:intron 20.0%
!!! AAAACCACAATTTAAAATCT+TGG - chr3_1:13015887-13015906 None:intergenic 20.0%
!! AAAATCAAAAATTTGTGGTT+TGG - chr3_1:13015922-13015941 None:intergenic 20.0%
!!! AGAAAATATATTTGATTTGC+AGG + chr3_1:13016208-13016227 Msa0349430:intron 20.0%
!! AAATTTCGAATTCAAAACTT+TGG + chr3_1:13016336-13016355 Msa0349430:intron 20.0%
!! AAAAATGTAACCTATAAAAG+TGG + chr3_1:13016584-13016603 Msa0349430:three_prime_UTR 20.0%
!!! TTTAATGTAACCACTTTTAT+AGG - chr3_1:13016597-13016616 None:intergenic 20.0%
!!! ATTAAAAATTGATGATGCAT+AGG + chr3_1:13016611-13016630 Msa0349430:three_prime_UTR 20.0%
!!! TTCAATTTTTGTCTCATAAA+TGG + chr3_1:13016745-13016764 Msa0349430:three_prime_UTR 20.0%
!! ACAAACAAAATGGAAAAATT+TGG - chr3_1:13016916-13016935 None:intergenic 20.0%
! AGAATGTTTGAGGATAATAA+GGG - chr3_1:13014767-13014786 None:intergenic 25.0%
! AAGAATGTTTGAGGATAATA+AGG - chr3_1:13014768-13014787 None:intergenic 25.0%
! AAAGAAGTTAAGAATGTTTG+AGG - chr3_1:13014777-13014796 None:intergenic 25.0%
! AACATTTACTGTCCAAATTA+TGG + chr3_1:13014813-13014832 Msa0349430:exon 25.0%
! AAAATTACCATGAAATGAAG+AGG - chr3_1:13014908-13014927 None:intergenic 25.0%
!!! ATATGATTATGTGGTTTGTT+TGG + chr3_1:13015167-13015186 Msa0349430:intron 25.0%
!! TTTTGTAATTTCAGTTACTG+TGG + chr3_1:13015371-13015390 Msa0349430:intron 25.0%
! TGCAAAAACAATTAACCAAA+AGG - chr3_1:13015587-13015606 None:intergenic 25.0%
!!! TTTTGGTTAATTGTTTTTGC+AGG + chr3_1:13015586-13015605 Msa0349430:intron 25.0%
! TGAAATTGACAAGACAATAA+TGG - chr3_1:13015772-13015791 None:intergenic 25.0%
!! GTCTTGTCAATTTCAAATTT+TGG + chr3_1:13015777-13015796 Msa0349430:intron 25.0%
!! ATCATCAGAATTTGAAATGA+TGG + chr3_1:13015800-13015819 Msa0349430:intron 25.0%
!! TCATCAGAATTTGAAATGAT+GGG + chr3_1:13015801-13015820 Msa0349430:intron 25.0%
!! ATGACCAAGATTTTAAATTG+TGG + chr3_1:13015880-13015899 Msa0349430:intron 25.0%
! TGGTGAAAATCAAAAATTTG+TGG - chr3_1:13015927-13015946 None:intergenic 25.0%
! TCACCAAAAAAGTAAAATTG+CGG + chr3_1:13015941-13015960 Msa0349430:intron 25.0%
!!! AAACCGCAATTTTACTTTTT+TGG - chr3_1:13015947-13015966 None:intergenic 25.0%
!!! CTATATTTTGATTGACAACA+AGG - chr3_1:13016029-13016048 None:intergenic 25.0%
!! CTTGTTGTCAATCAAAATAT+AGG + chr3_1:13016027-13016046 Msa0349430:intron 25.0%
! AGATTATAACTATCCCAATT+GGG - chr3_1:13016095-13016114 None:intergenic 25.0%
! TAGATTATAACTATCCCAAT+TGG - chr3_1:13016096-13016115 None:intergenic 25.0%
!! AATTTTGTCTTCAACAAATG+TGG - chr3_1:13016491-13016510 None:intergenic 25.0%
!!! TTTTTTGCCTAGTTTTTCAA+TGG - chr3_1:13016569-13016588 None:intergenic 25.0%
! ATAGGAGTTTACAAACAAAA+TGG - chr3_1:13016926-13016945 None:intergenic 25.0%
! AGAATGTTTGAGGATAATAA+GGG - chr3_1:13014767-13014786 None:intergenic 25.0%
! AAGAATGTTTGAGGATAATA+AGG - chr3_1:13014768-13014787 None:intergenic 25.0%
! AAAGAAGTTAAGAATGTTTG+AGG - chr3_1:13014777-13014796 None:intergenic 25.0%
! AACATTTACTGTCCAAATTA+TGG + chr3_1:13014813-13014832 Msa0349430:exon 25.0%
! AAAATTACCATGAAATGAAG+AGG - chr3_1:13014908-13014927 None:intergenic 25.0%
!!! ATATGATTATGTGGTTTGTT+TGG + chr3_1:13015167-13015186 Msa0349430:intron 25.0%
!! TTTTGTAATTTCAGTTACTG+TGG + chr3_1:13015371-13015390 Msa0349430:intron 25.0%
! TGCAAAAACAATTAACCAAA+AGG - chr3_1:13015587-13015606 None:intergenic 25.0%
!!! TTTTGGTTAATTGTTTTTGC+AGG + chr3_1:13015586-13015605 Msa0349430:intron 25.0%
! TGAAATTGACAAGACAATAA+TGG - chr3_1:13015772-13015791 None:intergenic 25.0%
!! GTCTTGTCAATTTCAAATTT+TGG + chr3_1:13015777-13015796 Msa0349430:intron 25.0%
!! ATCATCAGAATTTGAAATGA+TGG + chr3_1:13015800-13015819 Msa0349430:intron 25.0%
!! TCATCAGAATTTGAAATGAT+GGG + chr3_1:13015801-13015820 Msa0349430:intron 25.0%
!! ATGACCAAGATTTTAAATTG+TGG + chr3_1:13015880-13015899 Msa0349430:intron 25.0%
! TGGTGAAAATCAAAAATTTG+TGG - chr3_1:13015927-13015946 None:intergenic 25.0%
! TCACCAAAAAAGTAAAATTG+CGG + chr3_1:13015941-13015960 Msa0349430:intron 25.0%
!!! AAACCGCAATTTTACTTTTT+TGG - chr3_1:13015947-13015966 None:intergenic 25.0%
!!! CTATATTTTGATTGACAACA+AGG - chr3_1:13016029-13016048 None:intergenic 25.0%
!! CTTGTTGTCAATCAAAATAT+AGG + chr3_1:13016027-13016046 Msa0349430:intron 25.0%
! AGATTATAACTATCCCAATT+GGG - chr3_1:13016095-13016114 None:intergenic 25.0%
! TAGATTATAACTATCCCAAT+TGG - chr3_1:13016096-13016115 None:intergenic 25.0%
!! AATTTTGTCTTCAACAAATG+TGG - chr3_1:13016491-13016510 None:intergenic 25.0%
!!! TTTTTTGCCTAGTTTTTCAA+TGG - chr3_1:13016569-13016588 None:intergenic 25.0%
! ATAGGAGTTTACAAACAAAA+TGG - chr3_1:13016926-13016945 None:intergenic 25.0%
!! GAGGATGTCACATTTTAATA+GGG - chr3_1:13014702-13014721 None:intergenic 30.0%
!! AGAGGATGTCACATTTTAAT+AGG - chr3_1:13014703-13014722 None:intergenic 30.0%
AAGATAAAGATGAATGAGAG+AGG - chr3_1:13014721-13014740 None:intergenic 30.0%
TATGAAACTGTGCCATAATT+TGG - chr3_1:13014828-13014847 None:intergenic 30.0%
! TATGGCACAGTTTCATATTT+TGG + chr3_1:13014831-13014850 Msa0349430:exon 30.0%
! TTGACAGAATGTTGATTGAA+AGG + chr3_1:13014872-13014891 Msa0349430:CDS 30.0%
AGATGTTCCAAGTTATTACT+TGG + chr3_1:13015270-13015289 Msa0349430:intron 30.0%
!! GATGATGATGTTAATCCTTT+TGG + chr3_1:13015569-13015588 Msa0349430:intron 30.0%
CTCGATTATATGCAATACTA+AGG - chr3_1:13015979-13015998 None:intergenic 30.0%
GGATATCCAAAAAGGAAAAA+AGG - chr3_1:13016060-13016079 None:intergenic 30.0%
! TTTTTCCTTTTTGGATATCC+CGG + chr3_1:13016060-13016079 Msa0349430:intron 30.0%
TTTCGAATTCAAAACTTTGG+AGG + chr3_1:13016339-13016358 Msa0349430:intron 30.0%
TCTAAGCATCTGATTCAATA+AGG - chr3_1:13016407-13016426 None:intergenic 30.0%
TTAACATCTGATTGTTGAAC+TGG - chr3_1:13016458-13016477 None:intergenic 30.0%
ACCTAATCAACAACAAAAGA+AGG + chr3_1:13016525-13016544 Msa0349430:CDS 30.0%
!!! TCCTTCTTTTGTTGTTGATT+AGG - chr3_1:13016529-13016548 None:intergenic 30.0%
TGCAATTCCATTGAAAAACT+AGG + chr3_1:13016559-13016578 Msa0349430:CDS 30.0%
! ATAGGTCATTGATTGATAGT+AGG + chr3_1:13016699-13016718 Msa0349430:three_prime_UTR 30.0%
! TTTTGTCTCATAAATGGTAC+TGG + chr3_1:13016751-13016770 Msa0349430:three_prime_UTR 30.0%
! GTCAAGAATTTTCATGTGAT+TGG - chr3_1:13016871-13016890 None:intergenic 30.0%
!! GAGGATGTCACATTTTAATA+GGG - chr3_1:13014702-13014721 None:intergenic 30.0%
!! AGAGGATGTCACATTTTAAT+AGG - chr3_1:13014703-13014722 None:intergenic 30.0%
AAGATAAAGATGAATGAGAG+AGG - chr3_1:13014721-13014740 None:intergenic 30.0%
TATGAAACTGTGCCATAATT+TGG - chr3_1:13014828-13014847 None:intergenic 30.0%
! TATGGCACAGTTTCATATTT+TGG + chr3_1:13014831-13014850 Msa0349430:exon 30.0%
! TTGACAGAATGTTGATTGAA+AGG + chr3_1:13014872-13014891 Msa0349430:CDS 30.0%
AGATGTTCCAAGTTATTACT+TGG + chr3_1:13015270-13015289 Msa0349430:intron 30.0%
!! GATGATGATGTTAATCCTTT+TGG + chr3_1:13015569-13015588 Msa0349430:intron 30.0%
CTCGATTATATGCAATACTA+AGG - chr3_1:13015979-13015998 None:intergenic 30.0%
GGATATCCAAAAAGGAAAAA+AGG - chr3_1:13016060-13016079 None:intergenic 30.0%
! TTTTTCCTTTTTGGATATCC+CGG + chr3_1:13016060-13016079 Msa0349430:intron 30.0%
TTTCGAATTCAAAACTTTGG+AGG + chr3_1:13016339-13016358 Msa0349430:intron 30.0%
TCTAAGCATCTGATTCAATA+AGG - chr3_1:13016407-13016426 None:intergenic 30.0%
TTAACATCTGATTGTTGAAC+TGG - chr3_1:13016458-13016477 None:intergenic 30.0%
ACCTAATCAACAACAAAAGA+AGG + chr3_1:13016525-13016544 Msa0349430:CDS 30.0%
!!! TCCTTCTTTTGTTGTTGATT+AGG - chr3_1:13016529-13016548 None:intergenic 30.0%
TGCAATTCCATTGAAAAACT+AGG + chr3_1:13016559-13016578 Msa0349430:CDS 30.0%
! ATAGGTCATTGATTGATAGT+AGG + chr3_1:13016699-13016718 Msa0349430:three_prime_UTR 30.0%
! TTTTGTCTCATAAATGGTAC+TGG + chr3_1:13016751-13016770 Msa0349430:three_prime_UTR 30.0%
! GTCAAGAATTTTCATGTGAT+TGG - chr3_1:13016871-13016890 None:intergenic 30.0%
ATTCTGCAATGAAATCTGTC+AGG + chr3_1:13014612-13014631 Msa0349430:five_prime_UTR 35.0%
!! GATGTCACATTTTAATAGGG+TGG - chr3_1:13014699-13014718 None:intergenic 35.0%
TGTTTGAGGATAATAAGGGA+GGG - chr3_1:13014763-13014782 None:intergenic 35.0%
ATGTTTGAGGATAATAAGGG+AGG - chr3_1:13014764-13014783 None:intergenic 35.0%
CTCAAAACCTCTTCATTTCA+TGG + chr3_1:13014898-13014917 Msa0349430:CDS 35.0%
GTGTGTTCCAAGTAATAACT+TGG - chr3_1:13015280-13015299 None:intergenic 35.0%
!! ACTAAGGCTTTGAAGTTTCT+TGG + chr3_1:13015405-13015424 Msa0349430:CDS 35.0%
ATAACTGATTACTGTATGCC+AGG + chr3_1:13015629-13015648 Msa0349430:CDS 35.0%
TGATCTGCTGAGAAAAATCA+AGG + chr3_1:13015664-13015683 Msa0349430:CDS 35.0%
! TTTGTGGTTTGGAAAAATCG+CGG - chr3_1:13015911-13015930 None:intergenic 35.0%
!!! CGTAGACCTTTTTTCCTTTT+TGG + chr3_1:13016051-13016070 Msa0349430:intron 35.0%
TTCTCTTAAAGACATACCAG+TGG + chr3_1:13016238-13016257 Msa0349430:CDS 35.0%
ATGTTCCATCAAGGATTAAC+AGG + chr3_1:13016279-13016298 Msa0349430:CDS 35.0%
TGAATCAGATGCTTAGAAGA+AGG + chr3_1:13016410-13016429 Msa0349430:intron 35.0%
!! TTCTCACTAATTTGTTGGTG+AGG - chr3_1:13016665-13016684 None:intergenic 35.0%
! AGCTCTTCTCACTAATTTGT+TGG - chr3_1:13016670-13016689 None:intergenic 35.0%
!! ATTTTCATGTGATTGGTCCA+TGG - chr3_1:13016864-13016883 None:intergenic 35.0%
GGAAGAATCACATCATTTGT+TGG - chr3_1:13016895-13016914 None:intergenic 35.0%
ATTCTGCAATGAAATCTGTC+AGG + chr3_1:13014612-13014631 Msa0349430:five_prime_UTR 35.0%
!! GATGTCACATTTTAATAGGG+TGG - chr3_1:13014699-13014718 None:intergenic 35.0%
TGTTTGAGGATAATAAGGGA+GGG - chr3_1:13014763-13014782 None:intergenic 35.0%
ATGTTTGAGGATAATAAGGG+AGG - chr3_1:13014764-13014783 None:intergenic 35.0%
CTCAAAACCTCTTCATTTCA+TGG + chr3_1:13014898-13014917 Msa0349430:CDS 35.0%
GTGTGTTCCAAGTAATAACT+TGG - chr3_1:13015280-13015299 None:intergenic 35.0%
!! ACTAAGGCTTTGAAGTTTCT+TGG + chr3_1:13015405-13015424 Msa0349430:CDS 35.0%
ATAACTGATTACTGTATGCC+AGG + chr3_1:13015629-13015648 Msa0349430:CDS 35.0%
TGATCTGCTGAGAAAAATCA+AGG + chr3_1:13015664-13015683 Msa0349430:CDS 35.0%
! TTTGTGGTTTGGAAAAATCG+CGG - chr3_1:13015911-13015930 None:intergenic 35.0%
!!! CGTAGACCTTTTTTCCTTTT+TGG + chr3_1:13016051-13016070 Msa0349430:intron 35.0%
TTCTCTTAAAGACATACCAG+TGG + chr3_1:13016238-13016257 Msa0349430:CDS 35.0%
ATGTTCCATCAAGGATTAAC+AGG + chr3_1:13016279-13016298 Msa0349430:CDS 35.0%
TGAATCAGATGCTTAGAAGA+AGG + chr3_1:13016410-13016429 Msa0349430:intron 35.0%
!! TTCTCACTAATTTGTTGGTG+AGG - chr3_1:13016665-13016684 None:intergenic 35.0%
! AGCTCTTCTCACTAATTTGT+TGG - chr3_1:13016670-13016689 None:intergenic 35.0%
!! ATTTTCATGTGATTGGTCCA+TGG - chr3_1:13016864-13016883 None:intergenic 35.0%
GGAAGAATCACATCATTTGT+TGG - chr3_1:13016895-13016914 None:intergenic 35.0%
GATTTCATTGCAGAATCTGC+CGG - chr3_1:13014608-13014627 None:intergenic 40.0%
TCCAAACTGTCAGATTTCCT+AGG + chr3_1:13014644-13014663 Msa0349430:five_prime_UTR 40.0%
TCCTAGGAAATCTGACAGTT+TGG - chr3_1:13014648-13014667 None:intergenic 40.0%
AAACTGTCAGATTTCCTAGG+AGG + chr3_1:13014647-13014666 Msa0349430:five_prime_UTR 40.0%
AGGAGATACAAAATCCTCCT+AGG - chr3_1:13014664-13014683 None:intergenic 40.0%
TAGGGTGGACATCATATTCA+AGG - chr3_1:13014684-13014703 None:intergenic 40.0%
GTTTGAGGATAATAAGGGAG+GGG - chr3_1:13014762-13014781 None:intergenic 40.0%
TGGAACACACTTCATACAGA+TGG + chr3_1:13015290-13015309 Msa0349430:intron 40.0%
! TCAGTTACTGTGGTTGATTC+TGG + chr3_1:13015381-13015400 Msa0349430:intron 40.0%
! TGTGGTTGATTCTGGTACTA+AGG + chr3_1:13015389-13015408 Msa0349430:CDS 40.0%
!! GGCTTTGAAGTTTCTTGGTT+TGG + chr3_1:13015410-13015429 Msa0349430:CDS 40.0%
!! TCTTGGTTTGGTTGAAGATG+AGG + chr3_1:13015422-13015441 Msa0349430:CDS 40.0%
GTTTCTGCAGCAATAGTTTG+AGG - chr3_1:13015462-13015481 None:intergenic 40.0%
TATTGCTGCAGAAACAGATC+AGG + chr3_1:13015467-13015486 Msa0349430:CDS 40.0%
CATAACCGGGATATCCAAAA+AGG - chr3_1:13016068-13016087 None:intergenic 40.0%
GGTTACCTGTTAATCCTTGA+TGG - chr3_1:13016287-13016306 None:intergenic 40.0%
!! AGAAGAGCTTGCTGATGAAT+AGG + chr3_1:13016681-13016700 Msa0349430:three_prime_UTR 40.0%
TTGGTCCATGGATAACTCTT+GGG - chr3_1:13016852-13016871 None:intergenic 40.0%
ATTGGTCCATGGATAACTCT+TGG - chr3_1:13016853-13016872 None:intergenic 40.0%
GATTTCATTGCAGAATCTGC+CGG - chr3_1:13014608-13014627 None:intergenic 40.0%
TCCAAACTGTCAGATTTCCT+AGG + chr3_1:13014644-13014663 Msa0349430:five_prime_UTR 40.0%
TCCTAGGAAATCTGACAGTT+TGG - chr3_1:13014648-13014667 None:intergenic 40.0%
AAACTGTCAGATTTCCTAGG+AGG + chr3_1:13014647-13014666 Msa0349430:five_prime_UTR 40.0%
AGGAGATACAAAATCCTCCT+AGG - chr3_1:13014664-13014683 None:intergenic 40.0%
TAGGGTGGACATCATATTCA+AGG - chr3_1:13014684-13014703 None:intergenic 40.0%
GTTTGAGGATAATAAGGGAG+GGG - chr3_1:13014762-13014781 None:intergenic 40.0%
TGGAACACACTTCATACAGA+TGG + chr3_1:13015290-13015309 Msa0349430:intron 40.0%
! TCAGTTACTGTGGTTGATTC+TGG + chr3_1:13015381-13015400 Msa0349430:intron 40.0%
! TGTGGTTGATTCTGGTACTA+AGG + chr3_1:13015389-13015408 Msa0349430:CDS 40.0%
!! GGCTTTGAAGTTTCTTGGTT+TGG + chr3_1:13015410-13015429 Msa0349430:CDS 40.0%
!! TCTTGGTTTGGTTGAAGATG+AGG + chr3_1:13015422-13015441 Msa0349430:CDS 40.0%
GTTTCTGCAGCAATAGTTTG+AGG - chr3_1:13015462-13015481 None:intergenic 40.0%
TATTGCTGCAGAAACAGATC+AGG + chr3_1:13015467-13015486 Msa0349430:CDS 40.0%
CATAACCGGGATATCCAAAA+AGG - chr3_1:13016068-13016087 None:intergenic 40.0%
GGTTACCTGTTAATCCTTGA+TGG - chr3_1:13016287-13016306 None:intergenic 40.0%
!! AGAAGAGCTTGCTGATGAAT+AGG + chr3_1:13016681-13016700 Msa0349430:three_prime_UTR 40.0%
TTGGTCCATGGATAACTCTT+GGG - chr3_1:13016852-13016871 None:intergenic 40.0%
ATTGGTCCATGGATAACTCT+TGG - chr3_1:13016853-13016872 None:intergenic 40.0%
AAGGGAGGGGAAATAGAAGA+GGG - chr3_1:13014749-13014768 None:intergenic 45.0%
TAAGGGAGGGGAAATAGAAG+AGG - chr3_1:13014750-13014769 None:intergenic 45.0%
! ATGTTTATTTGGAACCGCCG+TGG + chr3_1:13015234-13015253 Msa0349430:intron 45.0%
ATCTCCCCTAAGGATTTCCA+CGG - chr3_1:13015254-13015273 None:intergenic 45.0%
AACTTGGAACATCTCCCCTA+AGG - chr3_1:13015264-13015283 None:intergenic 45.0%
ATACAGATGGATCTGCACTG+CGG + chr3_1:13015303-13015322 Msa0349430:intron 45.0%
GTTTGGTTGAAGATGAGGTG+AGG + chr3_1:13015427-13015446 Msa0349430:CDS 45.0%
TACTGTATGCCAGGAATGAC+AGG + chr3_1:13015638-13015657 Msa0349430:CDS 45.0%
AGATCATAGCCTGTCATTCC+TGG - chr3_1:13015650-13015669 None:intergenic 45.0%
CCAATTGGGATGTCATAACC+GGG - chr3_1:13016081-13016100 None:intergenic 45.0%
CCCAATTGGGATGTCATAAC+CGG - chr3_1:13016082-13016101 None:intergenic 45.0%
CCGGTTATGACATCCCAATT+GGG + chr3_1:13016079-13016098 Msa0349430:intron 45.0%
TCTGAGGACATAATCACCAC+TGG - chr3_1:13016257-13016276 None:intergenic 45.0%
CCTTGATGGAACATTCTCTG+AGG - chr3_1:13016273-13016292 None:intergenic 45.0%
CCTCAGAGAATGTTCCATCA+AGG + chr3_1:13016270-13016289 Msa0349430:CDS 45.0%
AAGGGAGGGGAAATAGAAGA+GGG - chr3_1:13014749-13014768 None:intergenic 45.0%
TAAGGGAGGGGAAATAGAAG+AGG - chr3_1:13014750-13014769 None:intergenic 45.0%
! ATGTTTATTTGGAACCGCCG+TGG + chr3_1:13015234-13015253 Msa0349430:intron 45.0%
ATCTCCCCTAAGGATTTCCA+CGG - chr3_1:13015254-13015273 None:intergenic 45.0%
AACTTGGAACATCTCCCCTA+AGG - chr3_1:13015264-13015283 None:intergenic 45.0%
ATACAGATGGATCTGCACTG+CGG + chr3_1:13015303-13015322 Msa0349430:intron 45.0%
GTTTGGTTGAAGATGAGGTG+AGG + chr3_1:13015427-13015446 Msa0349430:CDS 45.0%
TACTGTATGCCAGGAATGAC+AGG + chr3_1:13015638-13015657 Msa0349430:CDS 45.0%
AGATCATAGCCTGTCATTCC+TGG - chr3_1:13015650-13015669 None:intergenic 45.0%
CCAATTGGGATGTCATAACC+GGG - chr3_1:13016081-13016100 None:intergenic 45.0%
CCCAATTGGGATGTCATAAC+CGG - chr3_1:13016082-13016101 None:intergenic 45.0%
CCGGTTATGACATCCCAATT+GGG + chr3_1:13016079-13016098 Msa0349430:intron 45.0%
TCTGAGGACATAATCACCAC+TGG - chr3_1:13016257-13016276 None:intergenic 45.0%
CCTTGATGGAACATTCTCTG+AGG - chr3_1:13016273-13016292 None:intergenic 45.0%
CCTCAGAGAATGTTCCATCA+AGG + chr3_1:13016270-13016289 Msa0349430:CDS 45.0%
!!! AAATTTTTAACATTAATAAA+GGG - chr3_1:13015543-13015562 None:intergenic 5.0%
!!! AAAATTTTTAACATTAATAA+AGG - chr3_1:13015544-13015563 None:intergenic 5.0%
!!! TTTATTAATGTTAAAAATTT+TGG + chr3_1:13015543-13015562 Msa0349430:intron 5.0%
!!! AAATTTTTAACATTAATAAA+GGG - chr3_1:13015543-13015562 None:intergenic 5.0%
!!! AAAATTTTTAACATTAATAA+AGG - chr3_1:13015544-13015563 None:intergenic 5.0%
!!! TTTATTAATGTTAAAAATTT+TGG + chr3_1:13015543-13015562 Msa0349430:intron 5.0%
AACCGCCGTGGAAATCCTTA+GGG + chr3_1:13015246-13015265 Msa0349430:intron 50.0%
CCCGGTTATGACATCCCAAT+TGG + chr3_1:13016078-13016097 Msa0349430:intron 50.0%
TCCAGCCCAAGAGTTATCCA+TGG + chr3_1:13016844-13016863 Msa0349430:three_prime_UTR 50.0%
TCCATGGATAACTCTTGGGC+TGG - chr3_1:13016848-13016867 None:intergenic 50.0%
AACCGCCGTGGAAATCCTTA+GGG + chr3_1:13015246-13015265 Msa0349430:intron 50.0%
CCCGGTTATGACATCCCAAT+TGG + chr3_1:13016078-13016097 Msa0349430:intron 50.0%
TCCAGCCCAAGAGTTATCCA+TGG + chr3_1:13016844-13016863 Msa0349430:three_prime_UTR 50.0%
TCCATGGATAACTCTTGGGC+TGG - chr3_1:13016848-13016867 None:intergenic 50.0%
GAACCGCCGTGGAAATCCTT+AGG + chr3_1:13015245-13015264 Msa0349430:intron 55.0%
ACCGCCGTGGAAATCCTTAG+GGG + chr3_1:13015247-13015266 Msa0349430:intron 55.0%
TCCCCTAAGGATTTCCACGG+CGG - chr3_1:13015251-13015270 None:intergenic 55.0%
GAACCGCCGTGGAAATCCTT+AGG + chr3_1:13015245-13015264 Msa0349430:intron 55.0%
ACCGCCGTGGAAATCCTTAG+GGG + chr3_1:13015247-13015266 Msa0349430:intron 55.0%
TCCCCTAAGGATTTCCACGG+CGG - chr3_1:13015251-13015270 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 13014599 13016952 13014599 ID=Msa0349430;Name=Msa0349430
chr3_1 mRNA 13014599 13016952 13014599 ID=Msa0349430-mRNA-1;Parent=Msa0349430;Name=Msa0349430-mRNA-1;_AED=0.15;_eAED=0.15;_QI=233|1|1|1|1|1|5|372|167
chr3_1 exon 13014599 13014919 13014599 ID=Msa0349430-mRNA-1:exon:3325;Parent=Msa0349430-mRNA-1
chr3_1 exon 13015385 13015488 13015385 ID=Msa0349430-mRNA-1:exon:3326;Parent=Msa0349430-mRNA-1
chr3_1 exon 13015608 13015685 13015608 ID=Msa0349430-mRNA-1:exon:3327;Parent=Msa0349430-mRNA-1
chr3_1 exon 13016230 13016300 13016230 ID=Msa0349430-mRNA-1:exon:3328;Parent=Msa0349430-mRNA-1
chr3_1 exon 13016418 13016952 13016418 ID=Msa0349430-mRNA-1:exon:3329;Parent=Msa0349430-mRNA-1
chr3_1 five_prime_UTR 13014599 13014831 13014599 ID=Msa0349430-mRNA-1:five_prime_utr;Parent=Msa0349430-mRNA-1
chr3_1 CDS 13014832 13014919 13014832 ID=Msa0349430-mRNA-1:cds;Parent=Msa0349430-mRNA-1
chr3_1 CDS 13015385 13015488 13015385 ID=Msa0349430-mRNA-1:cds;Parent=Msa0349430-mRNA-1
chr3_1 CDS 13015608 13015685 13015608 ID=Msa0349430-mRNA-1:cds;Parent=Msa0349430-mRNA-1
chr3_1 CDS 13016230 13016300 13016230 ID=Msa0349430-mRNA-1:cds;Parent=Msa0349430-mRNA-1
chr3_1 CDS 13016418 13016580 13016418 ID=Msa0349430-mRNA-1:cds;Parent=Msa0349430-mRNA-1
chr3_1 three_prime_UTR 13016581 13016952 13016581 ID=Msa0349430-mRNA-1:three_prime_utr;Parent=Msa0349430-mRNA-1
Gene Sequence

>Msa0349430

ATGGCACAGTTTCATATTTTGGCTGTTGATGACAGTGTTATTGACAGAATGTTGATTGAAAGGCTGCTCAAAACCTCTTCATTTCATGTTACTGTGGTTGATTCTGGTACTAAGGCTTTGAAGTTTCTTGGTTTGGTTGAAGATGAGGTGAGGAATGAAAAGCCTCAAACTATTGCTGCAGAAACAGATCAGGTTGTAGAAGTGAATCTGATCATAACTGATTACTGTATGCCAGGAATGACAGGCTATGATCTGCTGAGAAAAATCAAGGAATCTAATTCTCTTAAAGACATACCAGTGGTGATTATGTCCTCAGAGAATGTTCCATCAAGGATTAACAGATGCTTAGAAGAAGGAGCTGAAGAATTCTTTCTGAAACCAGTTCAACAATCAGATGTTAACAAGTTGAAACCACATTTGTTGAAGACAAAATTTAAGAATGAAGATGACCTAATCAACAACAAAAGAAGGATACAAAACAATGCAATTCCATTGAAAAACTAG
Protein sequence

>Msa0349430

MAQFHILAVDDSVIDRMLIERLLKTSSFHVTVVDSGTKALKFLGLVEDEVRNEKPQTIAAETDQVVEVNLIITDYCMPGMTGYDLLRKIKESNSLKDIPVVIMSSENVPSRINRCLEEGAEEFFLKPVQQSDVNKLKPHLLKTKFKNEDDLINNKRRIQNNAIPLKN*