AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1151000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1151000 MtrunA17_Chr3g0082861 97.605 167 4 0 1 167 1 167 2.21e-119 333
Msa1151000 MtrunA17_Chr8g0352871 74.545 165 41 1 3 167 8 171 1.24e-84 246
Msa1151000 MtrunA17_Chr7g0256551 67.901 162 50 1 2 163 7 166 9.48e-78 231
Msa1151000 MtrunA17_Chr3g0124861 58.491 159 52 3 3 150 21 176 1.16e-58 182
Msa1151000 MtrunA17_Chr5g0414931 56.643 143 57 1 3 145 15 152 4.11e-53 168
Msa1151000 MtrunA17_Chr4g0059571 52.761 163 68 2 2 164 24 177 4.06e-52 165
Msa1151000 MtrunA17_Chr1g0170741 54.140 157 63 2 3 159 7 154 1.27e-51 163
Msa1151000 MtrunA17_Chr3g0118211 50.307 163 72 2 1 163 20 173 2.96e-51 162
Msa1151000 MtrunA17_Chr3g0128691 55.634 142 46 3 5 141 19 148 3.25e-50 158
Msa1151000 MtrunA17_Chr4g0021731 73.864 88 14 1 3 90 10 88 8.48e-41 132
Msa1151000 MtrunA17_Chr4g0030271 29.927 137 78 2 6 142 91 209 1.36e-14 70.5
Msa1151000 MtrunA17_Chr8g0345901 27.143 140 84 1 6 145 38 159 1.39e-14 70.5
Msa1151000 MtrunA17_Chr1g0181811 27.737 137 81 1 6 142 87 205 5.77e-13 65.9
Msa1151000 MtrunA17_Chr3g0127941 27.333 150 91 2 4 153 56 187 1.40e-12 64.7
Msa1151000 MtrunA17_Chr7g0276361 24.074 162 103 2 1 162 13 154 3.05e-12 63.5
Msa1151000 MtrunA17_Chr7g0275401 29.221 154 85 4 4 154 9 141 8.30e-12 62.0
Msa1151000 MtrunA17_Chr1g0161431 28.125 160 93 4 2 161 13 150 2.44e-11 61.2
Msa1151000 MtrunA17_Chr1g0149851 28.671 143 81 3 6 148 11 132 5.14e-11 60.1
Msa1151000 MtrunA17_Chr7g0275891 28.777 139 79 3 6 144 11 129 5.40e-11 59.7
Msa1151000 MtrunA17_Chr8g0376881 26.207 145 86 3 2 146 27 150 7.10e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1151000 AT3G57040.2 69.799 149 42 2 2 148 7 154 3.02e-71 215
Msa1151000 AT3G57040.1 69.799 149 42 2 2 148 7 154 3.02e-71 215
Msa1151000 AT2G41310.1 64.430 149 52 1 2 150 7 154 4.81e-67 204
Msa1151000 AT1G59940.1 57.233 159 54 4 3 160 32 177 2.12e-56 177
Msa1151000 AT1G59940.2 57.962 157 52 4 3 158 124 267 1.51e-54 175
Msa1151000 AT1G10470.3 55.556 162 57 5 1 161 17 164 3.06e-54 172
Msa1151000 AT1G10470.1 56.250 160 55 5 3 161 33 178 3.98e-54 172
Msa1151000 AT3G56380.1 55.634 142 55 2 3 144 19 152 3.06e-52 164
Msa1151000 AT3G48100.1 52.500 160 67 3 4 163 25 175 3.54e-52 164
Msa1151000 AT2G40670.1 53.521 142 60 1 3 144 28 163 2.95e-51 162
Msa1151000 AT5G62920.1 57.447 141 51 2 4 144 25 156 1.03e-50 161
Msa1151000 AT3G56380.2 55.245 143 55 3 3 144 19 153 1.74e-50 159
Msa1151000 AT2G40670.2 53.147 143 60 2 3 144 28 164 2.15e-49 157
Msa1151000 AT1G19050.1 50.000 158 69 2 3 159 23 171 3.69e-49 157
Msa1151000 AT1G74890.1 50.625 160 68 2 3 160 17 167 7.40e-49 157
Msa1151000 AT2G07440.1 66.667 60 20 0 91 150 9 68 4.17e-22 86.7
Msa1151000 AT1G10470.2 52.857 70 27 2 90 158 35 99 2.23e-16 73.2
Msa1151000 AT5G24470.1 28.777 139 79 3 6 144 54 172 5.08e-13 66.2
Msa1151000 AT5G60100.7 28.467 137 80 1 6 142 66 184 6.10e-12 63.2
Msa1151000 AT5G60100.6 28.467 137 80 1 6 142 66 184 6.10e-12 63.2
Msa1151000 AT5G60100.4 28.467 137 80 1 6 142 66 184 6.10e-12 63.2
Msa1151000 AT5G60100.2 28.467 137 80 1 6 142 66 184 6.41e-12 63.2
Msa1151000 AT5G60100.5 28.467 137 80 1 6 142 66 184 1.25e-11 62.4
Msa1151000 AT5G60100.3 28.467 137 80 1 6 142 66 184 1.25e-11 62.4
Msa1151000 AT5G60100.1 28.467 137 80 1 6 142 66 184 1.25e-11 62.4
Msa1151000 AT2G46790.3 26.619 139 84 2 4 142 37 157 1.88e-11 61.6
Msa1151000 AT5G07210.2 30.000 140 76 3 5 144 17 134 2.10e-11 61.6
Msa1151000 AT2G46790.1 26.619 139 84 2 4 142 37 157 2.10e-11 61.6
Msa1151000 AT5G02810.1 26.277 137 83 2 6 142 80 198 3.63e-11 60.8
Msa1151000 AT2G25180.1 27.451 153 89 4 6 157 19 150 4.04e-11 60.8

Find 49 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGCACAGTTTCATATTT+TGG 0.112277 7_4:-34987506 Msa1151000:CDS
AACATTTACTGTCCAAATTA+TGG 0.196728 7_4:-34987524 Msa1151000:exon
TATGAAACTGTGCCATAATT+TGG 0.209887 7_4:+34987512 None:intergenic
AAGAATGTTTGAGGATAATA+AGG 0.213912 7_4:+34987572 None:intergenic
TCAGTTACTGTGGTTGATTC+TGG 0.242120 7_4:-34986955 Msa1151000:intron
CTCAAAACCTCTTCATTTCA+TGG 0.264136 7_4:-34987439 Msa1151000:intron
GGCTTTGAAGTTTCTTGGTT+TGG 0.274386 7_4:-34986926 Msa1151000:CDS
ACTAAGGCTTTGAAGTTTCT+TGG 0.296459 7_4:-34986931 Msa1151000:CDS
TGTGGTTGATTCTGGTACTA+AGG 0.342411 7_4:-34986947 Msa1151000:CDS
TCCTAGGAAATCTGACAGTT+TGG 0.350175 7_4:+34987692 None:intergenic
TTAACATCTGATTGTTGAAC+TGG 0.359149 7_4:+34985909 None:intergenic
ATAGGAGTTTACAAACAAAA+TGG 0.363836 7_4:+34985412 None:intergenic
AAATTGAATCAAATTCTTCA+TGG 0.382858 7_4:+34985634 None:intergenic
CATATTGGACGGATAACTCT+TGG 0.385982 7_4:+34985514 None:intergenic
AGCTCTTCTCACTAATTTGT+TGG 0.387474 7_4:+34985697 None:intergenic
AAAGAAGTTAAGAATGTTTG+AGG 0.402917 7_4:+34987563 None:intergenic
GTCACATGTCAGTTTCAAAT+AGG 0.414453 7_4:+34985394 None:intergenic
GTTTCTGCAGCAATAGTTTG+AGG 0.420290 7_4:+34986877 None:intergenic
AGATCATAGCCAGTCATTCC+TGG 0.422849 7_4:+34986690 None:intergenic
TACTGTATGCCAGGAATGAC+TGG 0.429066 7_4:-34986699 Msa1151000:CDS
AGAAGAGCTTGCTGATGAAT+AGG 0.435827 7_4:-34985683 Msa1151000:three_prime_UTR
GGAAGAATCACATCATTTGT+TGG 0.438550 7_4:+34985443 None:intergenic
ATGTTCCATCAAGGATCAAC+AGG 0.450863 7_4:-34986083 Msa1151000:intron
ATAGGTCATTGATTGATAGT+AGG 0.451798 7_4:-34985665 Msa1151000:three_prime_UTR
ATATTGGACGGATAACTCTT+GGG 0.471923 7_4:+34985515 None:intergenic
TCCAAACTGTCAGATTTCCT+AGG 0.495654 7_4:-34987693 Msa1151000:five_prime_UTR
TTCTCACTAATTTGTTGGTG+AGG 0.504206 7_4:+34985702 None:intergenic
AGGAGATACAAAATCCTCCT+AGG 0.511110 7_4:+34987676 None:intergenic
TAGGGTGGACATCATATTCA+AGG 0.515357 7_4:+34987656 None:intergenic
TGAATCAGATGCTTAGAAGA+AGG 0.516221 7_4:-34985954 Msa1151000:intron
ATAACTGATTACTGTATGCC+AGG 0.518630 7_4:-34986708 Msa1151000:CDS
ACCTAATCAACAACAAAAGA+AGG 0.519626 7_4:-34985839 Msa1151000:CDS
AGAATGTTTGAGGATAATAA+GGG 0.521500 7_4:+34987573 None:intergenic
TTGACAGAATGTTGATTGAA+AGG 0.523765 7_4:-34987465 Msa1151000:CDS
TGGACGGATAACTCTTGGGC+TGG 0.533661 7_4:+34985519 None:intergenic
AAAATTACCATGAAATGAAG+AGG 0.534519 7_4:+34987432 None:intergenic
ATTCTGCAATGAAATCTGTC+AGG 0.543289 7_4:-34987725 Msa1151000:five_prime_UTR
GATTTCATTGCAGAATCTGC+CGG 0.543870 7_4:+34987732 None:intergenic
TATTGCTGCAGAAACAGATC+AGG 0.546049 7_4:-34986869 Msa1151000:intron
AAGATAAAGATGAATGAGAG+AGG 0.554093 7_4:+34987619 None:intergenic
TCTTGGTTTGGTTGAAGATG+AGG 0.555035 7_4:-34986914 Msa1151000:CDS
AAACTGTCAGATTTCCTAGG+AGG 0.585928 7_4:-34987690 Msa1151000:five_prime_UTR
CCTCAGAGAATGTTCCATCA+AGG 0.587746 7_4:-34986092 Msa1151000:CDS
TCTGAGGACATAATCACCAC+TGG 0.603381 7_4:+34986108 None:intergenic
GTTTGAGGATAATAAGGGAA+GGG 0.626959 7_4:+34987578 None:intergenic
TGTTTGAGGATAATAAGGGA+AGG 0.645942 7_4:+34987577 None:intergenic
GTTTGGTTGAAGATGAGGTG+AGG 0.669904 7_4:-34986909 Msa1151000:CDS
TTCTCTTAAAGACATACCAG+TGG 0.689344 7_4:-34986124 Msa1151000:CDS
CCTTGATGGAACATTCTCTG+AGG 0.696368 7_4:+34986092 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAAAAAATTTAGAAAATA+TGG - chr7_4:34985909-34985928 Msa1151000:CDS 10.0%
!!! CAAATTTTTAACATTAATAA+AGG + chr7_4:34986351-34986370 None:intergenic 10.0%
!! CTTTATTAATGTTAAAAATT+TGG - chr7_4:34986349-34986368 Msa1151000:intron 10.0%
!! CTTAAAAAATTTAGAAAATA+TGG - chr7_4:34985909-34985928 Msa1151000:CDS 10.0%
!!! CAAATTTTTAACATTAATAA+AGG + chr7_4:34986351-34986370 None:intergenic 10.0%
!! CTTTATTAATGTTAAAAATT+TGG - chr7_4:34986349-34986368 Msa1151000:intron 10.0%
!!! TATTTGAATTTTGATGTAAA+AGG + chr7_4:34985777-34985796 None:intergenic 15.0%
!!! ATTCAAATAATTTTGTGAAT+TGG - chr7_4:34985788-34985807 Msa1151000:exon 15.0%
!! ACAAAAATAATATGATTATG+TGG - chr7_4:34985964-34985983 Msa1151000:intron 15.0%
!!! AATTTTGAACGATTTTTTTT+TGG - chr7_4:34986009-34986028 Msa1151000:intron 15.0%
!! TGCAAAAATAATTAAGTAAA+AGG + chr7_4:34986393-34986412 None:intergenic 15.0%
!!! TTTTACTTAATTATTTTTGC+AGG - chr7_4:34986392-34986411 Msa1151000:intron 15.0%
!! AAATTTCAAATTCAAAACTT+TGG - chr7_4:34987115-34987134 Msa1151000:intron 15.0%
!!! TATTTGAATTTTGATGTAAA+AGG + chr7_4:34985777-34985796 None:intergenic 15.0%
!!! ATTCAAATAATTTTGTGAAT+TGG - chr7_4:34985788-34985807 Msa1151000:exon 15.0%
!! ACAAAAATAATATGATTATG+TGG - chr7_4:34985964-34985983 Msa1151000:intron 15.0%
!!! AATTTTGAACGATTTTTTTT+TGG - chr7_4:34986009-34986028 Msa1151000:intron 15.0%
!! TGCAAAAATAATTAAGTAAA+AGG + chr7_4:34986393-34986412 None:intergenic 15.0%
!!! TTTTACTTAATTATTTTTGC+AGG - chr7_4:34986392-34986411 Msa1151000:intron 15.0%
!! AAATTTCAAATTCAAAACTT+TGG - chr7_4:34987115-34987134 Msa1151000:intron 15.0%
!!! TTTTCTCTCATATTTTTTAG+AGG - chr7_4:34985733-34985752 Msa1151000:three_prime_UTR 20.0%
!!! AAATTTTGTAAAGAACAAAC+TGG + chr7_4:34985864-34985883 None:intergenic 20.0%
!! GGTAATATGTAATGTTTATT+TGG - chr7_4:34986030-34986049 Msa1151000:intron 20.0%
!! AATGATTAGACTAATACATA+TGG - chr7_4:34986313-34986332 Msa1151000:intron 20.0%
!! AAAAATCAAGGTAAATTCAT+AGG - chr7_4:34986482-34986501 Msa1151000:intron 20.0%
!!! ATGACCAATATTTTAAATTG+TGG - chr7_4:34986687-34986706 Msa1151000:CDS 20.0%
!!! AGAACCACAATTTAAAATAT+TGG + chr7_4:34986694-34986713 None:intergenic 20.0%
!!! AAAATATAACTTTTGTGGTT+TGG + chr7_4:34986729-34986748 None:intergenic 20.0%
!!! TTGTGAAAATATAACTTTTG+TGG + chr7_4:34986734-34986753 None:intergenic 20.0%
!! CTCGATTATATGAAATATTA+AGG + chr7_4:34986791-34986810 None:intergenic 20.0%
!! TATGGTTACTATCAAAAAAT+TGG + chr7_4:34986924-34986943 None:intergenic 20.0%
!!! ATACAAGCAATTTTATGTTA+TGG + chr7_4:34986942-34986961 None:intergenic 20.0%
!!! AGAAAATATATTTGATTTGC+AGG - chr7_4:34986989-34987008 Msa1151000:intron 20.0%
!! AAAAATGTAACCTATAAAAG+TGG - chr7_4:34987363-34987382 Msa1151000:intron 20.0%
!!! TTTAATGTAACCACTTTTAT+AGG + chr7_4:34987376-34987395 None:intergenic 20.0%
!!! ATTAAAAATTGATGATGCAT+AGG - chr7_4:34987390-34987409 Msa1151000:intron 20.0%
!! AAATTGAATCAAATTCTTCA+TGG + chr7_4:34987512-34987531 None:intergenic 20.0%
!!! TTCAATTTTTGTCTCATAAA+TGG - chr7_4:34987524-34987543 Msa1151000:exon 20.0%
!! TTAATGCATGAATTACATAT+TGG + chr7_4:34987647-34987666 None:intergenic 20.0%
!! ACAAACAAAATGGAAAAATT+TGG + chr7_4:34987724-34987743 None:intergenic 20.0%
!!! TTTTCTCTCATATTTTTTAG+AGG - chr7_4:34985733-34985752 Msa1151000:three_prime_UTR 20.0%
!!! AAATTTTGTAAAGAACAAAC+TGG + chr7_4:34985864-34985883 None:intergenic 20.0%
!! GGTAATATGTAATGTTTATT+TGG - chr7_4:34986030-34986049 Msa1151000:intron 20.0%
!! AATGATTAGACTAATACATA+TGG - chr7_4:34986313-34986332 Msa1151000:intron 20.0%
!! AAAAATCAAGGTAAATTCAT+AGG - chr7_4:34986482-34986501 Msa1151000:intron 20.0%
!!! ATGACCAATATTTTAAATTG+TGG - chr7_4:34986687-34986706 Msa1151000:CDS 20.0%
!!! AGAACCACAATTTAAAATAT+TGG + chr7_4:34986694-34986713 None:intergenic 20.0%
!!! AAAATATAACTTTTGTGGTT+TGG + chr7_4:34986729-34986748 None:intergenic 20.0%
!!! TTGTGAAAATATAACTTTTG+TGG + chr7_4:34986734-34986753 None:intergenic 20.0%
!! CTCGATTATATGAAATATTA+AGG + chr7_4:34986791-34986810 None:intergenic 20.0%
!! TATGGTTACTATCAAAAAAT+TGG + chr7_4:34986924-34986943 None:intergenic 20.0%
!!! ATACAAGCAATTTTATGTTA+TGG + chr7_4:34986942-34986961 None:intergenic 20.0%
!!! AGAAAATATATTTGATTTGC+AGG - chr7_4:34986989-34987008 Msa1151000:intron 20.0%
!! AAAAATGTAACCTATAAAAG+TGG - chr7_4:34987363-34987382 Msa1151000:intron 20.0%
!!! TTTAATGTAACCACTTTTAT+AGG + chr7_4:34987376-34987395 None:intergenic 20.0%
!!! ATTAAAAATTGATGATGCAT+AGG - chr7_4:34987390-34987409 Msa1151000:intron 20.0%
!! AAATTGAATCAAATTCTTCA+TGG + chr7_4:34987512-34987531 None:intergenic 20.0%
!!! TTCAATTTTTGTCTCATAAA+TGG - chr7_4:34987524-34987543 Msa1151000:exon 20.0%
!! TTAATGCATGAATTACATAT+TGG + chr7_4:34987647-34987666 None:intergenic 20.0%
!! ACAAACAAAATGGAAAAATT+TGG + chr7_4:34987724-34987743 None:intergenic 20.0%
! AGAATGTTTGAGGATAATAA+GGG + chr7_4:34985573-34985592 None:intergenic 25.0%
! AAGAATGTTTGAGGATAATA+AGG + chr7_4:34985574-34985593 None:intergenic 25.0%
! AAAGAAGTTAAGAATGTTTG+AGG + chr7_4:34985583-34985602 None:intergenic 25.0%
! AACATTTACTGTCCAAATTA+TGG - chr7_4:34985619-34985638 Msa1151000:three_prime_UTR 25.0%
! AAAATTACCATGAAATGAAG+AGG + chr7_4:34985714-34985733 None:intergenic 25.0%
!!! ATATGATTATGTGGTTTGTT+TGG - chr7_4:34985973-34985992 Msa1151000:intron 25.0%
!! TTTTGTAATTTCAGTTACTG+TGG - chr7_4:34986178-34986197 Msa1151000:intron 25.0%
!! ATCGTAGAATTTGAAATGAT+GGG - chr7_4:34986608-34986627 Msa1151000:intron 25.0%
! TTTCAAATTCAAAACTTTGG+AGG - chr7_4:34987118-34987137 Msa1151000:intron 25.0%
!!! TTTTTTGCCTAGTTTTTCAA+TGG + chr7_4:34987348-34987367 None:intergenic 25.0%
! ATAGGAGTTTACAAACAAAA+TGG + chr7_4:34987734-34987753 None:intergenic 25.0%
! AGAATGTTTGAGGATAATAA+GGG + chr7_4:34985573-34985592 None:intergenic 25.0%
! AAGAATGTTTGAGGATAATA+AGG + chr7_4:34985574-34985593 None:intergenic 25.0%
! AAAGAAGTTAAGAATGTTTG+AGG + chr7_4:34985583-34985602 None:intergenic 25.0%
! AACATTTACTGTCCAAATTA+TGG - chr7_4:34985619-34985638 Msa1151000:three_prime_UTR 25.0%
! AAAATTACCATGAAATGAAG+AGG + chr7_4:34985714-34985733 None:intergenic 25.0%
!!! ATATGATTATGTGGTTTGTT+TGG - chr7_4:34985973-34985992 Msa1151000:intron 25.0%
!! TTTTGTAATTTCAGTTACTG+TGG - chr7_4:34986178-34986197 Msa1151000:intron 25.0%
!! ATCGTAGAATTTGAAATGAT+GGG - chr7_4:34986608-34986627 Msa1151000:intron 25.0%
! TTTCAAATTCAAAACTTTGG+AGG - chr7_4:34987118-34987137 Msa1151000:intron 25.0%
!!! TTTTTTGCCTAGTTTTTCAA+TGG + chr7_4:34987348-34987367 None:intergenic 25.0%
! ATAGGAGTTTACAAACAAAA+TGG + chr7_4:34987734-34987753 None:intergenic 25.0%
!! GAGGATGTCACATTTTAATA+GGG + chr7_4:34985508-34985527 None:intergenic 30.0%
!! AGAGGATGTCACATTTTAAT+AGG + chr7_4:34985509-34985528 None:intergenic 30.0%
AAGATAAAGATGAATGAGAG+AGG + chr7_4:34985527-34985546 None:intergenic 30.0%
TATGAAACTGTGCCATAATT+TGG + chr7_4:34985634-34985653 None:intergenic 30.0%
! TATGGCACAGTTTCATATTT+TGG - chr7_4:34985637-34985656 Msa1151000:three_prime_UTR 30.0%
! TTGACAGAATGTTGATTGAA+AGG - chr7_4:34985678-34985697 Msa1151000:three_prime_UTR 30.0%
TGATGTTCCAAGTTATTACT+TGG - chr7_4:34986077-34986096 Msa1151000:intron 30.0%
! ATTCATAGGCTATTTGAAAC+TGG - chr7_4:34986496-34986515 Msa1151000:intron 30.0%
!! TATTTGAAACTGGTTTGTGT+TGG - chr7_4:34986506-34986525 Msa1151000:intron 30.0%
! GTCTTCGCAATTTCAAATTT+TGG - chr7_4:34986582-34986601 Msa1151000:intron 30.0%
! CATCGTAGAATTTGAAATGA+TGG - chr7_4:34986607-34986626 Msa1151000:intron 30.0%
CATATAATCGAGATCATATG+CGG - chr7_4:34986799-34986818 Msa1151000:intron 30.0%
!!! CGATTTTGATTATGACATCA+AGG + chr7_4:34986854-34986873 None:intergenic 30.0%
TGATGTCATAATCAAAATCG+AGG - chr7_4:34986854-34986873 Msa1151000:intron 30.0%
TCTAAGCATCTGATTCAATA+AGG + chr7_4:34987186-34987205 None:intergenic 30.0%
TTAACATCTGATTGTTGAAC+TGG + chr7_4:34987237-34987256 None:intergenic 30.0%
! ACTTTTGTCTTCAACAAATG+TGG + chr7_4:34987270-34987289 None:intergenic 30.0%
ACCTAATCAACAACAAAAGA+AGG - chr7_4:34987304-34987323 Msa1151000:intron 30.0%
!!! TCCTTCTTTTGTTGTTGATT+AGG + chr7_4:34987308-34987327 None:intergenic 30.0%
TGCAATTCCATTGAAAAACT+AGG - chr7_4:34987338-34987357 Msa1151000:intron 30.0%
! ATAGGTCATTGATTGATAGT+AGG - chr7_4:34987478-34987497 Msa1151000:CDS 30.0%
! TTTTGTCTCATAAATGGTAC+TGG - chr7_4:34987530-34987549 Msa1151000:five_prime_UTR 30.0%
TGCATGAATTACATATTGGA+CGG + chr7_4:34987643-34987662 None:intergenic 30.0%
TAATTCATGCATTAACTCCA+TGG - chr7_4:34987652-34987671 Msa1151000:five_prime_UTR 30.0%
! GTCAAGAATTTTCATGTGAT+TGG + chr7_4:34987679-34987698 None:intergenic 30.0%
!! GAGGATGTCACATTTTAATA+GGG + chr7_4:34985508-34985527 None:intergenic 30.0%
!! AGAGGATGTCACATTTTAAT+AGG + chr7_4:34985509-34985528 None:intergenic 30.0%
AAGATAAAGATGAATGAGAG+AGG + chr7_4:34985527-34985546 None:intergenic 30.0%
TATGAAACTGTGCCATAATT+TGG + chr7_4:34985634-34985653 None:intergenic 30.0%
! TATGGCACAGTTTCATATTT+TGG - chr7_4:34985637-34985656 Msa1151000:three_prime_UTR 30.0%
! TTGACAGAATGTTGATTGAA+AGG - chr7_4:34985678-34985697 Msa1151000:three_prime_UTR 30.0%
TGATGTTCCAAGTTATTACT+TGG - chr7_4:34986077-34986096 Msa1151000:intron 30.0%
! ATTCATAGGCTATTTGAAAC+TGG - chr7_4:34986496-34986515 Msa1151000:intron 30.0%
!! TATTTGAAACTGGTTTGTGT+TGG - chr7_4:34986506-34986525 Msa1151000:intron 30.0%
! GTCTTCGCAATTTCAAATTT+TGG - chr7_4:34986582-34986601 Msa1151000:intron 30.0%
! CATCGTAGAATTTGAAATGA+TGG - chr7_4:34986607-34986626 Msa1151000:intron 30.0%
CATATAATCGAGATCATATG+CGG - chr7_4:34986799-34986818 Msa1151000:intron 30.0%
!!! CGATTTTGATTATGACATCA+AGG + chr7_4:34986854-34986873 None:intergenic 30.0%
TGATGTCATAATCAAAATCG+AGG - chr7_4:34986854-34986873 Msa1151000:intron 30.0%
TCTAAGCATCTGATTCAATA+AGG + chr7_4:34987186-34987205 None:intergenic 30.0%
TTAACATCTGATTGTTGAAC+TGG + chr7_4:34987237-34987256 None:intergenic 30.0%
! ACTTTTGTCTTCAACAAATG+TGG + chr7_4:34987270-34987289 None:intergenic 30.0%
ACCTAATCAACAACAAAAGA+AGG - chr7_4:34987304-34987323 Msa1151000:intron 30.0%
!!! TCCTTCTTTTGTTGTTGATT+AGG + chr7_4:34987308-34987327 None:intergenic 30.0%
TGCAATTCCATTGAAAAACT+AGG - chr7_4:34987338-34987357 Msa1151000:intron 30.0%
! ATAGGTCATTGATTGATAGT+AGG - chr7_4:34987478-34987497 Msa1151000:CDS 30.0%
! TTTTGTCTCATAAATGGTAC+TGG - chr7_4:34987530-34987549 Msa1151000:five_prime_UTR 30.0%
TGCATGAATTACATATTGGA+CGG + chr7_4:34987643-34987662 None:intergenic 30.0%
TAATTCATGCATTAACTCCA+TGG - chr7_4:34987652-34987671 Msa1151000:five_prime_UTR 30.0%
! GTCAAGAATTTTCATGTGAT+TGG + chr7_4:34987679-34987698 None:intergenic 30.0%
ATTCTGCAATGAAATCTGTC+AGG - chr7_4:34985418-34985437 Msa1151000:three_prime_UTR 35.0%
!! GATGTCACATTTTAATAGGG+TGG + chr7_4:34985505-34985524 None:intergenic 35.0%
GTTTGAGGATAATAAGGGAA+GGG + chr7_4:34985568-34985587 None:intergenic 35.0%
TGTTTGAGGATAATAAGGGA+AGG + chr7_4:34985569-34985588 None:intergenic 35.0%
CTCAAAACCTCTTCATTTCA+TGG - chr7_4:34985704-34985723 Msa1151000:three_prime_UTR 35.0%
! ATCATCTCTAAGGATTTCCA+CGG + chr7_4:34986061-34986080 None:intergenic 35.0%
AACTTGGAACATCATCTCTA+AGG + chr7_4:34986071-34986090 None:intergenic 35.0%
GTGTGTTCCAAGTAATAACT+TGG + chr7_4:34986087-34986106 None:intergenic 35.0%
!! ACTAAGGCTTTGAAGTTTCT+TGG - chr7_4:34986212-34986231 Msa1151000:intron 35.0%
ATAACTGATTACTGTATGCC+AGG - chr7_4:34986435-34986454 Msa1151000:intron 35.0%
TGATCTGCTGAGAAAAATCA+AGG - chr7_4:34986470-34986489 Msa1151000:intron 35.0%
TTCTCTTAAAGACATACCAG+TGG - chr7_4:34987019-34987038 Msa1151000:intron 35.0%
! TTTTACCTGTTGATCCTTGA+TGG + chr7_4:34987068-34987087 None:intergenic 35.0%
TGAATCAGATGCTTAGAAGA+AGG - chr7_4:34987189-34987208 Msa1151000:intron 35.0%
!! TTCTCACTAATTTGTTGGTG+AGG + chr7_4:34987444-34987463 None:intergenic 35.0%
! AGCTCTTCTCACTAATTTGT+TGG + chr7_4:34987449-34987468 None:intergenic 35.0%
ATATTGGACGGATAACTCTT+GGG + chr7_4:34987631-34987650 None:intergenic 35.0%
!! ATTTTCATGTGATTGGTCCA+TGG + chr7_4:34987672-34987691 None:intergenic 35.0%
GGAAGAATCACATCATTTGT+TGG + chr7_4:34987703-34987722 None:intergenic 35.0%
ATTCTGCAATGAAATCTGTC+AGG - chr7_4:34985418-34985437 Msa1151000:three_prime_UTR 35.0%
!! GATGTCACATTTTAATAGGG+TGG + chr7_4:34985505-34985524 None:intergenic 35.0%
GTTTGAGGATAATAAGGGAA+GGG + chr7_4:34985568-34985587 None:intergenic 35.0%
TGTTTGAGGATAATAAGGGA+AGG + chr7_4:34985569-34985588 None:intergenic 35.0%
CTCAAAACCTCTTCATTTCA+TGG - chr7_4:34985704-34985723 Msa1151000:three_prime_UTR 35.0%
! ATCATCTCTAAGGATTTCCA+CGG + chr7_4:34986061-34986080 None:intergenic 35.0%
AACTTGGAACATCATCTCTA+AGG + chr7_4:34986071-34986090 None:intergenic 35.0%
GTGTGTTCCAAGTAATAACT+TGG + chr7_4:34986087-34986106 None:intergenic 35.0%
!! ACTAAGGCTTTGAAGTTTCT+TGG - chr7_4:34986212-34986231 Msa1151000:intron 35.0%
ATAACTGATTACTGTATGCC+AGG - chr7_4:34986435-34986454 Msa1151000:intron 35.0%
TGATCTGCTGAGAAAAATCA+AGG - chr7_4:34986470-34986489 Msa1151000:intron 35.0%
TTCTCTTAAAGACATACCAG+TGG - chr7_4:34987019-34987038 Msa1151000:intron 35.0%
! TTTTACCTGTTGATCCTTGA+TGG + chr7_4:34987068-34987087 None:intergenic 35.0%
TGAATCAGATGCTTAGAAGA+AGG - chr7_4:34987189-34987208 Msa1151000:intron 35.0%
!! TTCTCACTAATTTGTTGGTG+AGG + chr7_4:34987444-34987463 None:intergenic 35.0%
! AGCTCTTCTCACTAATTTGT+TGG + chr7_4:34987449-34987468 None:intergenic 35.0%
ATATTGGACGGATAACTCTT+GGG + chr7_4:34987631-34987650 None:intergenic 35.0%
!! ATTTTCATGTGATTGGTCCA+TGG + chr7_4:34987672-34987691 None:intergenic 35.0%
GGAAGAATCACATCATTTGT+TGG + chr7_4:34987703-34987722 None:intergenic 35.0%
GATTTCATTGCAGAATCTGC+CGG + chr7_4:34985414-34985433 None:intergenic 40.0%
TCCAAACTGTCAGATTTCCT+AGG - chr7_4:34985450-34985469 Msa1151000:three_prime_UTR 40.0%
TCCTAGGAAATCTGACAGTT+TGG + chr7_4:34985454-34985473 None:intergenic 40.0%
AAACTGTCAGATTTCCTAGG+AGG - chr7_4:34985453-34985472 Msa1151000:three_prime_UTR 40.0%
AGGAGATACAAAATCCTCCT+AGG + chr7_4:34985470-34985489 None:intergenic 40.0%
TAGGGTGGACATCATATTCA+AGG + chr7_4:34985490-34985509 None:intergenic 40.0%
TGGAACACACTTCATACAGA+TGG - chr7_4:34986097-34986116 Msa1151000:CDS 40.0%
! TCAGTTACTGTGGTTGATTC+TGG - chr7_4:34986188-34986207 Msa1151000:intron 40.0%
! TGTGGTTGATTCTGGTACTA+AGG - chr7_4:34986196-34986215 Msa1151000:intron 40.0%
!! GGCTTTGAAGTTTCTTGGTT+TGG - chr7_4:34986217-34986236 Msa1151000:intron 40.0%
!! TCTTGGTTTGGTTGAAGATG+AGG - chr7_4:34986229-34986248 Msa1151000:intron 40.0%
GTTTCTGCAGCAATAGTTTG+AGG + chr7_4:34986269-34986288 None:intergenic 40.0%
TATTGCTGCAGAAACAGATC+AGG - chr7_4:34986274-34986293 Msa1151000:intron 40.0%
! TTTGTGGTTTGGAGAAATCG+CGG + chr7_4:34986718-34986737 None:intergenic 40.0%
ATGTTCCATCAAGGATCAAC+AGG - chr7_4:34987060-34987079 Msa1151000:intron 40.0%
!! AGAAGAGCTTGCTGATGAAT+AGG - chr7_4:34987460-34987479 Msa1151000:CDS 40.0%
CATATTGGACGGATAACTCT+TGG + chr7_4:34987632-34987651 None:intergenic 40.0%
GATTTCATTGCAGAATCTGC+CGG + chr7_4:34985414-34985433 None:intergenic 40.0%
TCCAAACTGTCAGATTTCCT+AGG - chr7_4:34985450-34985469 Msa1151000:three_prime_UTR 40.0%
TCCTAGGAAATCTGACAGTT+TGG + chr7_4:34985454-34985473 None:intergenic 40.0%
AAACTGTCAGATTTCCTAGG+AGG - chr7_4:34985453-34985472 Msa1151000:three_prime_UTR 40.0%
AGGAGATACAAAATCCTCCT+AGG + chr7_4:34985470-34985489 None:intergenic 40.0%
TAGGGTGGACATCATATTCA+AGG + chr7_4:34985490-34985509 None:intergenic 40.0%
TGGAACACACTTCATACAGA+TGG - chr7_4:34986097-34986116 Msa1151000:CDS 40.0%
! TCAGTTACTGTGGTTGATTC+TGG - chr7_4:34986188-34986207 Msa1151000:intron 40.0%
! TGTGGTTGATTCTGGTACTA+AGG - chr7_4:34986196-34986215 Msa1151000:intron 40.0%
!! GGCTTTGAAGTTTCTTGGTT+TGG - chr7_4:34986217-34986236 Msa1151000:intron 40.0%
!! TCTTGGTTTGGTTGAAGATG+AGG - chr7_4:34986229-34986248 Msa1151000:intron 40.0%
GTTTCTGCAGCAATAGTTTG+AGG + chr7_4:34986269-34986288 None:intergenic 40.0%
TATTGCTGCAGAAACAGATC+AGG - chr7_4:34986274-34986293 Msa1151000:intron 40.0%
! TTTGTGGTTTGGAGAAATCG+CGG + chr7_4:34986718-34986737 None:intergenic 40.0%
ATGTTCCATCAAGGATCAAC+AGG - chr7_4:34987060-34987079 Msa1151000:intron 40.0%
!! AGAAGAGCTTGCTGATGAAT+AGG - chr7_4:34987460-34987479 Msa1151000:CDS 40.0%
CATATTGGACGGATAACTCT+TGG + chr7_4:34987632-34987651 None:intergenic 40.0%
! ATGTTTATTTGGAACCGCCG+TGG - chr7_4:34986041-34986060 Msa1151000:intron 45.0%
! ATCTCTAAGGATTTCCACGG+CGG + chr7_4:34986058-34986077 None:intergenic 45.0%
ATACAGATGGATCTGCACTG+CGG - chr7_4:34986110-34986129 Msa1151000:CDS 45.0%
GTTTGGTTGAAGATGAGGTG+AGG - chr7_4:34986234-34986253 Msa1151000:intron 45.0%
TACTGTATGCCAGGAATGAC+TGG - chr7_4:34986444-34986463 Msa1151000:intron 45.0%
AGATCATAGCCAGTCATTCC+TGG + chr7_4:34986456-34986475 None:intergenic 45.0%
TCTGAGGACATAATCACCAC+TGG + chr7_4:34987038-34987057 None:intergenic 45.0%
CCTTGATGGAACATTCTCTG+AGG + chr7_4:34987054-34987073 None:intergenic 45.0%
CCTCAGAGAATGTTCCATCA+AGG - chr7_4:34987051-34987070 Msa1151000:intron 45.0%
! ATGTTTATTTGGAACCGCCG+TGG - chr7_4:34986041-34986060 Msa1151000:intron 45.0%
! ATCTCTAAGGATTTCCACGG+CGG + chr7_4:34986058-34986077 None:intergenic 45.0%
ATACAGATGGATCTGCACTG+CGG - chr7_4:34986110-34986129 Msa1151000:CDS 45.0%
GTTTGGTTGAAGATGAGGTG+AGG - chr7_4:34986234-34986253 Msa1151000:intron 45.0%
TACTGTATGCCAGGAATGAC+TGG - chr7_4:34986444-34986463 Msa1151000:intron 45.0%
AGATCATAGCCAGTCATTCC+TGG + chr7_4:34986456-34986475 None:intergenic 45.0%
TCTGAGGACATAATCACCAC+TGG + chr7_4:34987038-34987057 None:intergenic 45.0%
CCTTGATGGAACATTCTCTG+AGG + chr7_4:34987054-34987073 None:intergenic 45.0%
CCTCAGAGAATGTTCCATCA+AGG - chr7_4:34987051-34987070 Msa1151000:intron 45.0%
!!! AAATTTTTAACATTAATAAA+GGG + chr7_4:34986350-34986369 None:intergenic 5.0%
!! AAAAAAAAAATTAAAATTGT+AGG - chr7_4:34986754-34986773 Msa1151000:intron 5.0%
!!! AAATTTTTAACATTAATAAA+GGG + chr7_4:34986350-34986369 None:intergenic 5.0%
!! AAAAAAAAAATTAAAATTGT+AGG - chr7_4:34986754-34986773 Msa1151000:intron 5.0%
TGGACGGATAACTCTTGGGC+TGG + chr7_4:34987627-34987646 None:intergenic 55.0%
TGGACGGATAACTCTTGGGC+TGG + chr7_4:34987627-34987646 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 34985405 34987760 34985405 ID=Msa1151000;Name=Msa1151000
chr7_4 mRNA 34985405 34987760 34985405 ID=Msa1151000-mRNA-1;Parent=Msa1151000;Name=Msa1151000-mRNA-1;_AED=0.14;_eAED=0.14;_QI=233|1|1|1|0.8|0.66|6|372|167
chr7_4 exon 34985405 34985492 34985405 ID=Msa1151000-mRNA-1:exon:8270;Parent=Msa1151000-mRNA-1
chr7_4 exon 34985522 34985968 34985522 ID=Msa1151000-mRNA-1:exon:8269;Parent=Msa1151000-mRNA-1
chr7_4 exon 34986084 34986154 34986084 ID=Msa1151000-mRNA-1:exon:8268;Parent=Msa1151000-mRNA-1
chr7_4 exon 34986674 34986751 34986674 ID=Msa1151000-mRNA-1:exon:8267;Parent=Msa1151000-mRNA-1
chr7_4 exon 34986870 34986973 34986870 ID=Msa1151000-mRNA-1:exon:8266;Parent=Msa1151000-mRNA-1
chr7_4 exon 34987440 34987760 34987440 ID=Msa1151000-mRNA-1:exon:8265;Parent=Msa1151000-mRNA-1
chr7_4 five_prime_UTR 34987528 34987760 34987528 ID=Msa1151000-mRNA-1:five_prime_utr;Parent=Msa1151000-mRNA-1
chr7_4 CDS 34987440 34987527 34987440 ID=Msa1151000-mRNA-1:cds;Parent=Msa1151000-mRNA-1
chr7_4 CDS 34986870 34986973 34986870 ID=Msa1151000-mRNA-1:cds;Parent=Msa1151000-mRNA-1
chr7_4 CDS 34986674 34986751 34986674 ID=Msa1151000-mRNA-1:cds;Parent=Msa1151000-mRNA-1
chr7_4 CDS 34986084 34986154 34986084 ID=Msa1151000-mRNA-1:cds;Parent=Msa1151000-mRNA-1
chr7_4 CDS 34985806 34985968 34985806 ID=Msa1151000-mRNA-1:cds;Parent=Msa1151000-mRNA-1
chr7_4 three_prime_UTR 34985522 34985805 34985522 ID=Msa1151000-mRNA-1:three_prime_utr;Parent=Msa1151000-mRNA-1
chr7_4 three_prime_UTR 34985405 34985492 34985405 ID=Msa1151000-mRNA-1:three_prime_utr;Parent=Msa1151000-mRNA-1
Gene Sequence

>Msa1151000

ATGGCACAGTTTCATATTTTGGCTGTTGATGACAGTGTTATTGACAGAATGTTGATTGAAAGGCTGCTCAAAACCTCTTCATTTCATGTTACTGTGGTTGATTCTGGTACTAAGGCTTTGAAGTTTCTTGGTTTGGTTGAAGATGAGGTGAGGAATGAAAAGCCTCAAACTATTGCTGCAGAAACAGATCAGGTTGTAGAAGTGAATCTGATCATAACTGATTACTGTATGCCAGGAATGACTGGCTATGATCTGCTGAGAAAAATCAAGGAATCTAATTCTCTTAAAGACATACCAGTGGTGATTATGTCCTCAGAGAATGTTCCATCAAGGATCAACAGATGCTTAGAAGAAGGAGCTGAAGAATTCTTTCTGAAACCAGTTCAACAATCAGATGTTAACAAGTTGAAACCACATTTGTTGAAGACAAAAGTTAAGAATGAAGATGACCTAATCAACAACAAAAGAAGGATACAAAACAATGCAATTCCATTGAAAAACTAG
Protein sequence

>Msa1151000

MAQFHILAVDDSVIDRMLIERLLKTSSFHVTVVDSGTKALKFLGLVEDEVRNEKPQTIAAETDQVVEVNLIITDYCMPGMTGYDLLRKIKESNSLKDIPVVIMSSENVPSRINRCLEEGAEEFFLKPVQQSDVNKLKPHLLKTKVKNEDDLINNKRRIQNNAIPLKN*