Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0353340 | XP_024633996.1 | 89.394 | 66 | 7 | 0 | 1 | 66 | 1 | 66 | 2.38e-32 | 116 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0353340 | G7IZF3 | 89.394 | 66 | 7 | 0 | 1 | 66 | 1 | 66 | 1.14e-32 | 116 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0353340 | Msa0399940 | 0.887264 | 1.661833e-72 | -8.615850e-47 |
| Msa0353340 | Msa0427560 | 0.816642 | 4.978321e-52 | -8.615850e-47 |
| Msa0353340 | Msa0481770 | 0.810965 | 8.867322e-51 | -8.615850e-47 |
| Msa0353340 | Msa0660650 | 0.816752 | 4.703133e-52 | -8.615850e-47 |
| Msa0353340 | Msa1375890 | 0.802850 | 4.613497e-49 | -8.615850e-47 |
| Msa0353340 | Msa1453600 | 0.806885 | 6.620473e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTCGTTGGCTCCGGCACC+AGG | 0.351170 | 3_1:-23181501 | None:intergenic |
| GGTCCTGATGCTGGAGCTGC+TGG | 0.374025 | 3_1:-23181480 | None:intergenic |
| CCGGCACCAGGTCCTGATGC+TGG | 0.402695 | 3_1:-23181489 | None:intergenic |
| GATCCAGCAGCTCCAGCATC+AGG | 0.425147 | 3_1:+23181477 | Msa0353340:CDS |
| CCAACGAGGGACTAAGGTCT+TGG | 0.426576 | 3_1:+23181514 | Msa0353340:CDS |
| GGCCACTGTTGCGGCAAAGA+TGG | 0.471206 | 3_1:+23181542 | Msa0353340:CDS |
| CCGGAGCCAACGAGGGACTA+AGG | 0.496351 | 3_1:+23181508 | Msa0353340:CDS |
| CCAGCATCAGGACCTGGTGC+CGG | 0.497543 | 3_1:+23181489 | Msa0353340:CDS |
| CAACGAGGGACTAAGGTCTT+GGG | 0.507904 | 3_1:+23181515 | Msa0353340:CDS |
| GGGACTAAGGTCTTGGGCTG+AGG | 0.527888 | 3_1:+23181521 | Msa0353340:CDS |
| GCAGCTCCAGCATCAGGACC+TGG | 0.528586 | 3_1:+23181483 | Msa0353340:CDS |
| TCTATTTCATATATTTGCAA+TGG | 0.531028 | 3_1:-23181604 | None:intergenic |
| CCTTAGTCCCTCGTTGGCTC+CGG | 0.543980 | 3_1:-23181508 | None:intergenic |
| CGCCATCTTTGCCGCAACAG+TGG | 0.545743 | 3_1:-23181544 | None:intergenic |
| ACAAGTTCAGTGGCTATGAT+CGG | 0.577020 | 3_1:-23181450 | None:intergenic |
| TTGGGCTGAGGCCACTGTTG+CGG | 0.580861 | 3_1:+23181533 | Msa0353340:CDS |
| CCAAGACCTTAGTCCCTCGT+TGG | 0.585989 | 3_1:-23181514 | None:intergenic |
| CCTGGTGCCGGAGCCAACGA+GGG | 0.637311 | 3_1:+23181501 | Msa0353340:CDS |
| ACCTGGTGCCGGAGCCAACG+AGG | 0.655600 | 3_1:+23181500 | Msa0353340:CDS |
| TGGATCTGTTACAAGTTCAG+TGG | 0.658686 | 3_1:-23181460 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGATGGCGATGAAAAAGA+AGG | + | chr3_1:23181557-23181576 | Msa0353340:CDS | 35.0% | |
| ACAAGTTCAGTGGCTATGAT+CGG | - | chr3_1:23181453-23181472 | None:intergenic | 40.0% | |
| TGGATCTGTTACAAGTTCAG+TGG | - | chr3_1:23181463-23181482 | None:intergenic | 40.0% | |
| CAACGAGGGACTAAGGTCTT+GGG | + | chr3_1:23181515-23181534 | Msa0353340:CDS | 50.0% | |
| CCAAGACCTTAGTCCCTCGT+TGG | - | chr3_1:23181517-23181536 | None:intergenic | 55.0% | |
| CCAACGAGGGACTAAGGTCT+TGG | + | chr3_1:23181514-23181533 | Msa0353340:CDS | 55.0% | |
| GATCCAGCAGCTCCAGCATC+AGG | + | chr3_1:23181477-23181496 | Msa0353340:CDS | 60.0% | |
| !! | CCTTAGTCCCTCGTTGGCTC+CGG | - | chr3_1:23181511-23181530 | None:intergenic | 60.0% |
| GGGACTAAGGTCTTGGGCTG+AGG | + | chr3_1:23181521-23181540 | Msa0353340:CDS | 60.0% | |
| TTGGGCTGAGGCCACTGTTG+CGG | + | chr3_1:23181533-23181552 | Msa0353340:CDS | 60.0% | |
| GGCCACTGTTGCGGCAAAGA+TGG | + | chr3_1:23181542-23181561 | Msa0353340:CDS | 60.0% | |
| CGCCATCTTTGCCGCAACAG+TGG | - | chr3_1:23181547-23181566 | None:intergenic | 60.0% | |
| GGTCCTGATGCTGGAGCTGC+TGG | - | chr3_1:23181483-23181502 | None:intergenic | 65.0% | |
| GCAGCTCCAGCATCAGGACC+TGG | + | chr3_1:23181483-23181502 | Msa0353340:CDS | 65.0% | |
| CCAGCATCAGGACCTGGTGC+CGG | + | chr3_1:23181489-23181508 | Msa0353340:CDS | 65.0% | |
| CCGGAGCCAACGAGGGACTA+AGG | + | chr3_1:23181508-23181527 | Msa0353340:CDS | 65.0% | |
| CCGGCACCAGGTCCTGATGC+TGG | - | chr3_1:23181492-23181511 | None:intergenic | 70.0% | |
| ! | ACCTGGTGCCGGAGCCAACG+AGG | + | chr3_1:23181500-23181519 | Msa0353340:CDS | 70.0% |
| ! | CCTGGTGCCGGAGCCAACGA+GGG | + | chr3_1:23181501-23181520 | Msa0353340:CDS | 70.0% |
| !! | CCCTCGTTGGCTCCGGCACC+AGG | - | chr3_1:23181504-23181523 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 23181420 | 23181620 | 23181420 | ID=Msa0353340;Name=Msa0353340 |
| chr3_1 | mRNA | 23181420 | 23181620 | 23181420 | ID=Msa0353340-mRNA-1;Parent=Msa0353340;Name=Msa0353340-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|66 |
| chr3_1 | exon | 23181420 | 23181620 | 23181420 | ID=Msa0353340-mRNA-1:exon:5208;Parent=Msa0353340-mRNA-1 |
| chr3_1 | CDS | 23181420 | 23181620 | 23181420 | ID=Msa0353340-mRNA-1:cds;Parent=Msa0353340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
