Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0399940 | XP_024633996.1 | 90.909 | 66 | 6 | 0 | 1 | 66 | 1 | 66 | 6.73e-33 | 118 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0399940 | G7IZF3 | 90.909 | 66 | 6 | 0 | 1 | 66 | 1 | 66 | 3.21e-33 | 118 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0399940 | Msa0427560 | 0.826457 | 2.684014e-54 | -8.615850e-47 |
| Msa0399940 | Msa0481770 | 0.821209 | 4.560156e-53 | -8.615850e-47 |
| Msa0399940 | Msa0660650 | 0.824310 | 8.651982e-54 | -8.615850e-47 |
| Msa0399940 | Msa1375890 | 0.819516 | 1.114650e-52 | -8.615850e-47 |
| Msa0399940 | Msa1453600 | 0.811775 | 5.915108e-51 | -8.615850e-47 |
| Msa0353340 | Msa0399940 | 0.887264 | 1.661833e-72 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTCGTTGGCTCCGGCACC+AGG | 0.351170 | 3_2:-23746356 | None:intergenic |
| GGTCCTGATGCTGGAGCTGC+TGG | 0.374025 | 3_2:-23746335 | None:intergenic |
| CCAACGAGGGACTAAGATCT+TGG | 0.389978 | 3_2:+23746369 | Msa0399940:CDS |
| CCGGCACCAGGTCCTGATGC+TGG | 0.402695 | 3_2:-23746344 | None:intergenic |
| GATCCAGCAGCTCCAGCATC+AGG | 0.425147 | 3_2:+23746332 | Msa0399940:CDS |
| GGCCACTGTTGCGGCAAAGA+TGG | 0.471206 | 3_2:+23746397 | Msa0399940:CDS |
| CCAGCATCAGGACCTGGTGC+CGG | 0.497543 | 3_2:+23746344 | Msa0399940:CDS |
| CAACGAGGGACTAAGATCTT+GGG | 0.519181 | 3_2:+23746370 | Msa0399940:CDS |
| TGTATTTCATATATTTGCAA+TGG | 0.527746 | 3_2:-23746459 | None:intergenic |
| GCAGCTCCAGCATCAGGACC+TGG | 0.528586 | 3_2:+23746338 | Msa0399940:CDS |
| CGCCATCTTTGCCGCAACAG+TGG | 0.545743 | 3_2:-23746399 | None:intergenic |
| TCTTAGTCCCTCGTTGGCTC+CGG | 0.555270 | 3_2:-23746363 | None:intergenic |
| ACAAGTTCAGTGGCTATGAT+CGG | 0.577020 | 3_2:-23746305 | None:intergenic |
| TTGGGCTGAGGCCACTGTTG+CGG | 0.580861 | 3_2:+23746388 | Msa0399940:CDS |
| CCAAGATCTTAGTCCCTCGT+TGG | 0.585989 | 3_2:-23746369 | None:intergenic |
| GGGACTAAGATCTTGGGCTG+AGG | 0.619302 | 3_2:+23746376 | Msa0399940:CDS |
| CCTGGTGCCGGAGCCAACGA+GGG | 0.637311 | 3_2:+23746356 | Msa0399940:CDS |
| ACCTGGTGCCGGAGCCAACG+AGG | 0.655600 | 3_2:+23746355 | Msa0399940:CDS |
| TGGATCTGTTACAAGTTCAG+TGG | 0.658686 | 3_2:-23746315 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGATGGCGATGAAAAAGA+AGG | + | chr3_2:23746412-23746431 | Msa0399940:CDS | 35.0% | |
| ACAAGTTCAGTGGCTATGAT+CGG | - | chr3_2:23746308-23746327 | None:intergenic | 40.0% | |
| TGGATCTGTTACAAGTTCAG+TGG | - | chr3_2:23746318-23746337 | None:intergenic | 40.0% | |
| CAACGAGGGACTAAGATCTT+GGG | + | chr3_2:23746370-23746389 | Msa0399940:CDS | 45.0% | |
| CCAAGATCTTAGTCCCTCGT+TGG | - | chr3_2:23746372-23746391 | None:intergenic | 50.0% | |
| CCAACGAGGGACTAAGATCT+TGG | + | chr3_2:23746369-23746388 | Msa0399940:CDS | 50.0% | |
| !! | TCTTAGTCCCTCGTTGGCTC+CGG | - | chr3_2:23746366-23746385 | None:intergenic | 55.0% |
| GGGACTAAGATCTTGGGCTG+AGG | + | chr3_2:23746376-23746395 | Msa0399940:CDS | 55.0% | |
| GATCCAGCAGCTCCAGCATC+AGG | + | chr3_2:23746332-23746351 | Msa0399940:CDS | 60.0% | |
| TTGGGCTGAGGCCACTGTTG+CGG | + | chr3_2:23746388-23746407 | Msa0399940:CDS | 60.0% | |
| GGCCACTGTTGCGGCAAAGA+TGG | + | chr3_2:23746397-23746416 | Msa0399940:CDS | 60.0% | |
| CGCCATCTTTGCCGCAACAG+TGG | - | chr3_2:23746402-23746421 | None:intergenic | 60.0% | |
| GGTCCTGATGCTGGAGCTGC+TGG | - | chr3_2:23746338-23746357 | None:intergenic | 65.0% | |
| GCAGCTCCAGCATCAGGACC+TGG | + | chr3_2:23746338-23746357 | Msa0399940:CDS | 65.0% | |
| CCAGCATCAGGACCTGGTGC+CGG | + | chr3_2:23746344-23746363 | Msa0399940:CDS | 65.0% | |
| CCGGCACCAGGTCCTGATGC+TGG | - | chr3_2:23746347-23746366 | None:intergenic | 70.0% | |
| ! | ACCTGGTGCCGGAGCCAACG+AGG | + | chr3_2:23746355-23746374 | Msa0399940:CDS | 70.0% |
| ! | CCTGGTGCCGGAGCCAACGA+GGG | + | chr3_2:23746356-23746375 | Msa0399940:CDS | 70.0% |
| !! | CCCTCGTTGGCTCCGGCACC+AGG | - | chr3_2:23746359-23746378 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 23746275 | 23746475 | 23746275 | ID=Msa0399940;Name=Msa0399940 |
| chr3_2 | mRNA | 23746275 | 23746475 | 23746275 | ID=Msa0399940-mRNA-1;Parent=Msa0399940;Name=Msa0399940-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|66 |
| chr3_2 | exon | 23746275 | 23746475 | 23746275 | ID=Msa0399940-mRNA-1:exon:4997;Parent=Msa0399940-mRNA-1 |
| chr3_2 | CDS | 23746275 | 23746475 | 23746275 | ID=Msa0399940-mRNA-1:cds;Parent=Msa0399940-mRNA-1 |
| Gene Sequence |
| Protein sequence |
