AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0099910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099910 MtrunA17_Chr8g0347021 99.160 119 1 0 60 178 28 146 2.74e-83 242
Msa0099910 MtrunA17_Chr8g0347341 100.000 118 0 0 61 178 179 296 8.40e-81 241
Msa0099910 MtrunA17_Chr8g0347341 92.063 63 5 0 1 63 1 63 5.38e-34 121
Msa0099910 MtrunA17_Chr1g0150351 90.984 122 0 1 1 111 1 122 1.25e-74 220
Msa0099910 MtrunA17_Chr8g0347031 93.651 63 4 0 1 63 1 63 1.67e-36 121
Msa0099910 MtrunA17_Chr8g0347041 92.063 63 5 0 1 63 1 63 1.70e-34 121
Msa0099910 MtrunA17_Chr8g0347041 92.157 51 0 1 61 111 179 225 7.24e-24 94.0
Msa0099910 MtrunA17_Chr1g0150341 98.182 55 1 0 112 166 7 61 4.80e-33 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099910 AT5G10780.1 81.768 181 29 2 1 178 1 180 3.52e-88 256
Msa0099910 AT5G10780.2 82.840 169 25 2 1 166 1 168 8.18e-82 240

Find 93 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTCTTGGAGGAATTTA+AGG 0.091841 1_3:+6266121 Msa0099910:three_prime_UTR
GGTATTCATCGCCCTTAATT+TGG 0.174653 1_3:+6264895 Msa0099910:CDS
AACACTCTGTAAGGCACTTA+TGG 0.194375 1_3:-6263955 None:intergenic
TACTGCAAATCTGATGTAAA+AGG 0.202542 1_3:+6266364 Msa0099910:three_prime_UTR
ATCATTAACAAGTTCTTAAA+TGG 0.219625 1_3:-6263855 None:intergenic
GTAACATGCCTCCCAAATTA+AGG 0.244098 1_3:-6264907 None:intergenic
CTTGGAGGAATTTAAGGATT+TGG 0.280619 1_3:+6266127 Msa0099910:three_prime_UTR
CTTCACAATGGGAAGGTTAC+AGG 0.295260 1_3:-6266499 None:intergenic
TGAAGCAGGTATACAAAATA+TGG 0.299528 1_3:+6266517 Msa0099910:three_prime_UTR
TGGATTCAGCATCTTTCTTT+TGG 0.301710 1_3:-6263363 None:intergenic
CTGAAAGAACAATAAGAATT+CGG 0.312632 1_3:-6266444 None:intergenic
TCTTCTGCAGCTCAATTCAT+TGG 0.332546 1_3:+6265305 Msa0099910:intron
CTTTGTATCTCTTTGTATGT+AGG 0.333936 1_3:+6266302 Msa0099910:three_prime_UTR
GTATCACCTTCTCAGCTCTT+TGG 0.334333 1_3:+6263928 Msa0099910:CDS
AAGGGTGTGATGGGATCTTC+TGG 0.346236 1_3:+6263232 Msa0099910:CDS
GTATTCATCGCCCTTAATTT+GGG 0.348550 1_3:+6264896 Msa0099910:CDS
TACAAACCATGTCTAATCTT+TGG 0.367592 1_3:-6266240 None:intergenic
CTCTTTCATTCATTCATAGT+TGG 0.368002 1_3:+6263117 None:intergenic
GATGCTAAAGTGGAGCTTCT+TGG 0.369452 1_3:+6264863 Msa0099910:CDS
AATAGCATACTAGAAATGTT+AGG 0.385259 1_3:+6266388 Msa0099910:three_prime_UTR
ATTTAAGAACTTGTTAATGA+TGG 0.394212 1_3:+6263857 Msa0099910:CDS
ATCGCGAGAAGTTGAATTAT+CGG 0.394794 1_3:-6263278 None:intergenic
GAGGGAAGCGCGTGAGAAAC+CGG 0.398618 1_3:-6263314 None:intergenic
AATTTGGGAGGCATGTTACT+AGG 0.402445 1_3:+6264911 Msa0099910:CDS
GAGGCATGTTACTAGGTGTT+TGG 0.405795 1_3:+6264918 Msa0099910:CDS
CGATTCAATTCAATGGATAA+AGG 0.406344 1_3:+6263208 Msa0099910:exon
ATGCTAAAGTGGAGCTTCTT+GGG 0.409851 1_3:+6264864 Msa0099910:CDS
TCCACTTTAGCATCTTTGTA+AGG 0.413449 1_3:-6264854 None:intergenic
TGAAAGAACAATAAGAATTC+GGG 0.413709 1_3:-6266443 None:intergenic
CTTCTGCAGCTCAATTCATT+GGG 0.415950 1_3:+6265306 Msa0099910:intron
TCTTCAGCGATTCAATTCAA+TGG 0.418706 1_3:+6263201 Msa0099910:exon
TGGTCACAAATGATCTCATT+AGG 0.422294 1_3:+6266169 Msa0099910:three_prime_UTR
AAGAATGAGTGAAGAGAAGA+AGG 0.424461 1_3:-6263182 None:intergenic
ATTGTTGGAAACAGAAAGCT+TGG 0.427332 1_3:+6263817 Msa0099910:intron
ACACTCTGTAAGGCACTTAT+GGG 0.435288 1_3:-6263954 None:intergenic
TTTAAGAACTTGTTAATGAT+GGG 0.445845 1_3:+6263858 Msa0099910:CDS
AGAAAGATGCTGAATCCAAT+TGG 0.446637 1_3:+6263368 Msa0099910:CDS
TAACCTTCCCATTGTGAAGC+AGG 0.447117 1_3:+6266503 Msa0099910:three_prime_UTR
ATAGCATACTAGAAATGTTA+GGG 0.456027 1_3:+6266389 Msa0099910:three_prime_UTR
TAACATGCCTCCCAAATTAA+GGG 0.464691 1_3:-6264906 None:intergenic
GGGTTTATGATGTGGATGGC+TGG 0.475763 1_3:+6263879 Msa0099910:CDS
AACATAGACATTTAAAAGAA+AGG 0.477427 1_3:-6266470 None:intergenic
TTGTATACCTGCTTCACAAT+GGG 0.483638 1_3:-6266510 None:intergenic
TTCCTACCCACACATCAGAC+TGG 0.494006 1_3:+6265334 Msa0099910:CDS
TGGGATCTTCTGGACGAAGA+TGG 0.499581 1_3:+6263242 Msa0099910:CDS
TGAATCCAATTGGAAATCTC+AGG 0.499594 1_3:+6263378 Msa0099910:CDS
AAAGAAAATCCAACCTGAGC+AGG 0.500189 1_3:-6265372 None:intergenic
TCTTTGGTGACTGCAAGTAT+TGG 0.501455 1_3:-6266224 None:intergenic
CAGGAGGTGGAGTACTCAGG+TGG 0.509179 1_3:+6266056 Msa0099910:intron
GTCATCCTTAGCACCTGCTC+AGG 0.516236 1_3:+6265359 Msa0099910:CDS
GTACTGTTAGCATGTTGTAA+TGG 0.518335 1_3:+6266149 Msa0099910:three_prime_UTR
GATAAAGGAAAGGGTGTGAT+GGG 0.521901 1_3:+6263223 Msa0099910:CDS
CTTCAGGAGGTGGAGTACTC+AGG 0.524189 1_3:+6266053 Msa0099910:intron
GAGGTGGAGTACTCAGGTGG+GGG 0.524742 1_3:+6266059 Msa0099910:CDS
GGAGCTTCTTGGGCCTAAGT+TGG 0.528352 1_3:+6264874 Msa0099910:CDS
CGCGATATTATCGATCCTCC+CGG 0.529746 1_3:+6263295 Msa0099910:CDS
ACCTTACAAAGATGCTAAAG+TGG 0.529869 1_3:+6264853 Msa0099910:CDS
GGGATCTTCTGGACGAAGAT+GGG 0.531311 1_3:+6263243 Msa0099910:CDS
ACTGTTCACTTGTTTAGCAT+CGG 0.532087 1_3:+6263906 Msa0099910:CDS
GCATACCTGAGATTTCCAAT+TGG 0.533753 1_3:-6263383 None:intergenic
AGGAGGTGGAGTACTCAGGT+GGG 0.535718 1_3:+6266057 Msa0099910:intron
CATGTTACTAGGTGTTTGGA+AGG 0.537062 1_3:+6264922 Msa0099910:CDS
GAAAGCTTGGGAAGTTGCAC+AGG 0.537408 1_3:+6263830 Msa0099910:intron
TCCAACCTGAGCAGGTGCTA+AGG 0.537452 1_3:-6265364 None:intergenic
CAGTCACCAAAGATTAGACA+TGG 0.538585 1_3:+6266234 Msa0099910:three_prime_UTR
GGATAAAGGAAAGGGTGTGA+TGG 0.543038 1_3:+6263222 Msa0099910:CDS
CTTAAGAACTGGAGCATCAA+CGG 0.547901 1_3:-6266091 None:intergenic
AATTCAATGGATAAAGGAAA+GGG 0.549939 1_3:+6263214 Msa0099910:exon
GGAGGTGGAGTACTCAGGTG+GGG 0.550312 1_3:+6266058 Msa0099910:intron
TCCTACCCACACATCAGACT+GGG 0.552562 1_3:+6265335 Msa0099910:CDS
AGGGCGATGAATACCAACTT+AGG 0.558217 1_3:-6264887 None:intergenic
AGGGAAGCGCGTGAGAAACC+GGG 0.558868 1_3:-6263313 None:intergenic
CAATTCAATGGATAAAGGAA+AGG 0.565610 1_3:+6263213 Msa0099910:exon
TTGTTGGAAACAGAAAGCTT+GGG 0.565750 1_3:+6263818 Msa0099910:intron
TTAAGAACTTGTTAATGATG+GGG 0.568371 1_3:+6263859 Msa0099910:CDS
TTTGTATACCTGCTTCACAA+TGG 0.573399 1_3:-6266511 None:intergenic
TTCATCGCCCTTAATTTGGG+AGG 0.578157 1_3:+6264899 Msa0099910:CDS
GGGTTGCCAAAGAGCTGAGA+AGG 0.582231 1_3:-6263934 None:intergenic
GGTAGAATCGTCTTGATCGA+GGG 0.585755 1_3:-6263332 None:intergenic
AGAATGAGTGAAGAGAAGAA+GGG 0.591696 1_3:-6263181 None:intergenic
TAATGATGGGGTTTATGATG+TGG 0.592983 1_3:+6263871 Msa0099910:CDS
ATAAGTGCCTTACAGAGTGT+TGG 0.594768 1_3:+6263957 Msa0099910:CDS
GGGTAGAATCGTCTTGATCG+AGG 0.613210 1_3:-6263333 None:intergenic
TGAATTATCGGAGAAATCAA+CGG 0.616548 1_3:-6263266 None:intergenic
ATACCTGCTTCACAATGGGA+AGG 0.628040 1_3:-6266506 None:intergenic
ACATACAAAGAGATACAAAG+AGG 0.639704 1_3:-6266299 None:intergenic
GATGGGGTTTATGATGTGGA+TGG 0.644891 1_3:+6263875 Msa0099910:CDS
CATACTAGAAATGTTAGGGG+AGG 0.666754 1_3:+6266393 Msa0099910:three_prime_UTR
TGACACCCAGTCTGATGTGT+GGG 0.677476 1_3:-6265340 None:intergenic
TAGCATACTAGAAATGTTAG+GGG 0.703626 1_3:+6266390 Msa0099910:three_prime_UTR
ATGACACCCAGTCTGATGTG+TGG 0.709294 1_3:-6265341 None:intergenic
GAAGCGCGTGAGAAACCGGG+AGG 0.725314 1_3:-6263310 None:intergenic
ACCCAGTCTGATGTGTGGGT+AGG 0.740029 1_3:-6265336 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGATTAATTATATTGT+TGG + chr1_3:6263802-6263821 Msa0099910:intron 10.0%
!! GAAAAATAATAATAAGTATA+TGG - chr1_3:6265264-6265283 None:intergenic 10.0%
!! AAAATACAGAAATTATACAT+AGG - chr1_3:6263459-6263478 None:intergenic 15.0%
!! TATTTAACAAAACAACAAAA+AGG - chr1_3:6264017-6264036 None:intergenic 15.0%
!! TTTATATTATGGATGAAATT+AGG + chr1_3:6264237-6264256 Msa0099910:intron 15.0%
!! TATGTTCTATAATATATTTG+AGG + chr1_3:6265174-6265193 Msa0099910:intron 15.0%
!! TTGTATGTTATTAAAATGTT+TGG + chr1_3:6265565-6265584 Msa0099910:intron 15.0%
!! TGTATGTTATTAAAATGTTT+GGG + chr1_3:6265566-6265585 Msa0099910:intron 15.0%
!! TTTGAAAATAAATCTATAAC+TGG + chr1_3:6265905-6265924 Msa0099910:intron 15.0%
!!! AAAAAAAATCTATAACTTGT+TGG + chr1_3:6265938-6265957 Msa0099910:intron 15.0%
!! AGTTACAATAATTAGTTACA+TGG - chr1_3:6263728-6263747 None:intergenic 20.0%
!! ATCATTAACAAGTTCTTAAA+TGG - chr1_3:6263858-6263877 None:intergenic 20.0%
!!! ATTTAAGAACTTGTTAATGA+TGG + chr1_3:6263857-6263876 Msa0099910:CDS 20.0%
!!! TTTAAGAACTTGTTAATGAT+GGG + chr1_3:6263858-6263877 Msa0099910:CDS 20.0%
!! CAATGTTGATATTATTAGAA+TGG - chr1_3:6264099-6264118 None:intergenic 20.0%
!! ATACAATTACAATCATTGAA+TGG - chr1_3:6264151-6264170 None:intergenic 20.0%
!!! GAGGTTGATTATTTATATTA+TGG + chr1_3:6264226-6264245 Msa0099910:intron 20.0%
!!! CTTAATATGTATTTGTTGAA+TGG + chr1_3:6264513-6264532 Msa0099910:intron 20.0%
!!! GAACACTTTATTTTAAATTG+TGG + chr1_3:6264669-6264688 Msa0099910:intron 20.0%
!!! TTTAACTCATTTTTGTTTCA+AGG - chr1_3:6264958-6264977 None:intergenic 20.0%
!!! TTACCTCAAATTTTAAGTTT+TGG + chr1_3:6265119-6265138 Msa0099910:intron 20.0%
!!! CTATAATATATTTGAGGTTT+AGG + chr1_3:6265180-6265199 Msa0099910:intron 20.0%
!! TTAATAACATACAATGCTAT+GGG - chr1_3:6265559-6265578 None:intergenic 20.0%
!! TTTAATAACATACAATGCTA+TGG - chr1_3:6265560-6265579 None:intergenic 20.0%
!!! GGTTTTTTTTTTTTTTTCCT+GGG - chr1_3:6265608-6265627 None:intergenic 20.0%
!!! TGGTTTTTTTTTTTTTTTCC+TGG - chr1_3:6265609-6265628 None:intergenic 20.0%
!! GAAAAAAAAAAAAAAACCAG+AGG + chr1_3:6265610-6265629 Msa0099910:intron 20.0%
!!! CATTATTTCTGATATTAAAG+TGG - chr1_3:6265879-6265898 None:intergenic 20.0%
!!! AGTTATAGATTTTTTTTCAG+TGG - chr1_3:6265935-6265954 None:intergenic 20.0%
!! AACATAGACATTTAAAAGAA+AGG - chr1_3:6266473-6266492 None:intergenic 20.0%
! AATTCAATGGATAAAGGAAA+GGG + chr1_3:6263214-6263233 Msa0099910:exon 25.0%
! TCAACTGATAATAAACGAAA+GGG - chr1_3:6263593-6263612 None:intergenic 25.0%
! TAACAATCGTAAATAGTTTG+TGG - chr1_3:6263635-6263654 None:intergenic 25.0%
! CATCTGATTGTAATTGTTTA+AGG + chr1_3:6263756-6263775 Msa0099910:intron 25.0%
!!! TTAAGAACTTGTTAATGATG+GGG + chr1_3:6263859-6263878 Msa0099910:CDS 25.0%
! AAAGAAAAATAAAGTCGAAC+TGG + chr1_3:6264335-6264354 Msa0099910:intron 25.0%
! ATCTTATGAAAACAACGTAT+AGG - chr1_3:6264371-6264390 None:intergenic 25.0%
! TATAAGCTGAAAACAACTTA+TGG + chr1_3:6264410-6264429 Msa0099910:intron 25.0%
!! TATTTGTTGAATGGAATCAT+TGG + chr1_3:6264522-6264541 Msa0099910:intron 25.0%
! AAAATATGTAATTGCGTGTT+TGG + chr1_3:6264608-6264627 Msa0099910:intron 25.0%
! TTTCAAGAATTGTGAAAAGA+TGG + chr1_3:6264762-6264781 Msa0099910:intron 25.0%
! ATTGATAAACTCTTGATTCT+AGG + chr1_3:6265052-6265071 Msa0099910:intron 25.0%
! TTGATAAACTCTTGATTCTA+GGG + chr1_3:6265053-6265072 Msa0099910:intron 25.0%
! ACAAAACCATGTATATACTT+AGG - chr1_3:6265099-6265118 None:intergenic 25.0%
! GAACCAAAACTTAAAATTTG+AGG - chr1_3:6265125-6265144 None:intergenic 25.0%
!!! TAAGTTTTGGTTCATTTTGT+TGG + chr1_3:6265132-6265151 Msa0099910:intron 25.0%
! ATTCTCAAGTTTGAAAATCT+TGG + chr1_3:6265236-6265255 Msa0099910:intron 25.0%
!! TTCAGAGGACTAAAATATTT+TGG - chr1_3:6265436-6265455 None:intergenic 25.0%
!!! CGTTTTAATTAATGTTTCAG+AGG - chr1_3:6265451-6265470 None:intergenic 25.0%
!! ACATAATTTTACTGTCTTCA+AGG + chr1_3:6265476-6265495 Msa0099910:intron 25.0%
!! TTGCTTTATTGAGATGAAAT+TGG + chr1_3:6265667-6265686 Msa0099910:intron 25.0%
! ATTTATACACCAAATGATTG+TGG - chr1_3:6265765-6265784 None:intergenic 25.0%
! ACTTAAAGAAAAGAAACCAA+GGG - chr1_3:6266009-6266028 None:intergenic 25.0%
! AAGACAAAAAACTTAAGAAC+TGG - chr1_3:6266105-6266124 None:intergenic 25.0%
!!! TTAAGTTTTTTGTCTTCTCT+TGG + chr1_3:6266109-6266128 Msa0099910:three_prime_UTR 25.0%
! AATAGCATACTAGAAATGTT+AGG + chr1_3:6266388-6266407 Msa0099910:three_prime_UTR 25.0%
! ATAGCATACTAGAAATGTTA+GGG + chr1_3:6266389-6266408 Msa0099910:three_prime_UTR 25.0%
! TGAAAGAACAATAAGAATTC+GGG - chr1_3:6266446-6266465 None:intergenic 25.0%
! CTGAAAGAACAATAAGAATT+CGG - chr1_3:6266447-6266466 None:intergenic 25.0%
CGATTCAATTCAATGGATAA+AGG + chr1_3:6263208-6263227 Msa0099910:exon 30.0%
CAATTCAATGGATAAAGGAA+AGG + chr1_3:6263213-6263232 Msa0099910:exon 30.0%
TGAATTATCGGAGAAATCAA+CGG - chr1_3:6263269-6263288 None:intergenic 30.0%
CTCAACTGATAATAAACGAA+AGG - chr1_3:6263594-6263613 None:intergenic 30.0%
TGGAGTAGTACTTATTAGAT+CGG - chr1_3:6263708-6263727 None:intergenic 30.0%
ACAAAACAACAAAAAGGAAG+CGG - chr1_3:6264011-6264030 None:intergenic 30.0%
! TGGATGAAATTAGGTTTGTT+TGG + chr1_3:6264246-6264265 Msa0099910:intron 30.0%
ATACATATTAAGACCCTGTT+TGG - chr1_3:6264505-6264524 None:intergenic 30.0%
!! TTGGTTTGTTGTAACTCATT+AGG + chr1_3:6264573-6264592 Msa0099910:intron 30.0%
! CTTGCGATGTTAAATCTTTT+AGG + chr1_3:6264692-6264711 Msa0099910:intron 30.0%
TATCAGCCTAAGTATATACA+TGG + chr1_3:6265090-6265109 Msa0099910:intron 30.0%
! GTTCATTTTGTTGGATAGTT+GGG + chr1_3:6265141-6265160 Msa0099910:intron 30.0%
CTTACTACAAGTAATTTCTG+TGG + chr1_3:6265712-6265731 Msa0099910:intron 30.0%
GTGGTTCATCTATATTATTC+TGG - chr1_3:6265746-6265765 None:intergenic 30.0%
ATAGATGAACCACAATCATT+TGG + chr1_3:6265753-6265772 Msa0099910:intron 30.0%
AATCTACCAATTCTTCACTA+TGG - chr1_3:6265801-6265820 None:intergenic 30.0%
AAATCACAAAAGGATGAAGT+AGG - chr1_3:6265971-6265990 None:intergenic 30.0%
CACTTAAAGAAAAGAAACCA+AGG - chr1_3:6266010-6266029 None:intergenic 30.0%
CCTGAAGATACAAAAAAGTA+AGG - chr1_3:6266040-6266059 None:intergenic 30.0%
!!! CCTTACTTTTTTGTATCTTC+AGG + chr1_3:6266037-6266056 Msa0099910:intron 30.0%
!!! TACTTTTTTGTATCTTCAGG+AGG + chr1_3:6266040-6266059 Msa0099910:intron 30.0%
TACAAACCATGTCTAATCTT+TGG - chr1_3:6266243-6266262 None:intergenic 30.0%
ACATACAAAGAGATACAAAG+AGG - chr1_3:6266302-6266321 None:intergenic 30.0%
CTTTGTATCTCTTTGTATGT+AGG + chr1_3:6266302-6266321 Msa0099910:three_prime_UTR 30.0%
TACTGCAAATCTGATGTAAA+AGG + chr1_3:6266364-6266383 Msa0099910:three_prime_UTR 30.0%
TAGCATACTAGAAATGTTAG+GGG + chr1_3:6266390-6266409 Msa0099910:three_prime_UTR 30.0%
!!! AGGAGAATTTTTTTAGCAGT+GGG + chr1_3:6266413-6266432 Msa0099910:three_prime_UTR 30.0%
TGAAGCAGGTATACAAAATA+TGG + chr1_3:6266517-6266536 Msa0099910:three_prime_UTR 30.0%
AGAATGAGTGAAGAGAAGAA+GGG - chr1_3:6263184-6263203 None:intergenic 35.0%
AAGAATGAGTGAAGAGAAGA+AGG - chr1_3:6263185-6263204 None:intergenic 35.0%
TCTTCAGCGATTCAATTCAA+TGG + chr1_3:6263201-6263220 Msa0099910:exon 35.0%
ATCGCGAGAAGTTGAATTAT+CGG - chr1_3:6263281-6263300 None:intergenic 35.0%
! ATCTTTCTTTTGGCGACTAA+GGG - chr1_3:6263356-6263375 None:intergenic 35.0%
! TGGATTCAGCATCTTTCTTT+TGG - chr1_3:6263366-6263385 None:intergenic 35.0%
AGAAAGATGCTGAATCCAAT+TGG + chr1_3:6263368-6263387 Msa0099910:CDS 35.0%
TGAATCCAATTGGAAATCTC+AGG + chr1_3:6263378-6263397 Msa0099910:CDS 35.0%
ACTGATAATAAACGAAAGGG+AGG - chr1_3:6263590-6263609 None:intergenic 35.0%
ATTGTTGGAAACAGAAAGCT+TGG + chr1_3:6263817-6263836 Msa0099910:intron 35.0%
TTGTTGGAAACAGAAAGCTT+GGG + chr1_3:6263818-6263837 Msa0099910:intron 35.0%
! TAATGATGGGGTTTATGATG+TGG + chr1_3:6263871-6263890 Msa0099910:CDS 35.0%
ACTGTTCACTTGTTTAGCAT+CGG + chr1_3:6263906-6263925 Msa0099910:CDS 35.0%
!! ACTTTTTCCAACACTCTGTA+AGG - chr1_3:6263967-6263986 None:intergenic 35.0%
TTGAATGGAGAGATTCAGTA+AGG - chr1_3:6264136-6264155 None:intergenic 35.0%
CTTGATGTCTATAGATAACG+AGG + chr1_3:6264207-6264226 Msa0099910:intron 35.0%
AAGATATCCTAGTGAGCTTA+TGG + chr1_3:6264386-6264405 Msa0099910:intron 35.0%
CTTATATCCATAAGCTCACT+AGG - chr1_3:6264396-6264415 None:intergenic 35.0%
! GTCAGTCTCATAATTGCTTT+TGG + chr1_3:6264453-6264472 Msa0099910:intron 35.0%
TCAAATAAGTCGATCCAAAC+AGG + chr1_3:6264488-6264507 Msa0099910:intron 35.0%
CAAATAAGTCGATCCAAACA+GGG + chr1_3:6264489-6264508 Msa0099910:intron 35.0%
AACCAAAAGAGAAGAGAACT+GGG - chr1_3:6264559-6264578 None:intergenic 35.0%
AAACCAAAAGAGAAGAGAAC+TGG - chr1_3:6264560-6264579 None:intergenic 35.0%
!!! TCTTTTAGGACCTTTGCAAT+TGG + chr1_3:6264706-6264725 Msa0099910:intron 35.0%
ACCTTACAAAGATGCTAAAG+TGG + chr1_3:6264853-6264872 Msa0099910:CDS 35.0%
TCCACTTTAGCATCTTTGTA+AGG - chr1_3:6264857-6264876 None:intergenic 35.0%
GTATTCATCGCCCTTAATTT+GGG + chr1_3:6264896-6264915 Msa0099910:CDS 35.0%
TAACATGCCTCCCAAATTAA+GGG - chr1_3:6264909-6264928 None:intergenic 35.0%
! GGTTCATTTTGTTGGATAGT+TGG + chr1_3:6265140-6265159 Msa0099910:intron 35.0%
AGGCAGTAACATGAATAACA+GGG - chr1_3:6265286-6265305 None:intergenic 35.0%
! CTTCAAGGTATTATGACAAG+AGG + chr1_3:6265491-6265510 Msa0099910:intron 35.0%
TAACATACAATGCTATGGGT+AGG - chr1_3:6265555-6265574 None:intergenic 35.0%
CATATGCCATAGTGAAGAAT+TGG + chr1_3:6265792-6265811 Msa0099910:intron 35.0%
!!! TTTTTTGTATCTTCAGGAGG+TGG + chr1_3:6266043-6266062 Msa0099910:intron 35.0%
!!! AGTTTTTTGTCTTCTCTTGG+AGG + chr1_3:6266112-6266131 Msa0099910:three_prime_UTR 35.0%
TCTTCTCTTGGAGGAATTTA+AGG + chr1_3:6266121-6266140 Msa0099910:three_prime_UTR 35.0%
CTTGGAGGAATTTAAGGATT+TGG + chr1_3:6266127-6266146 Msa0099910:three_prime_UTR 35.0%
GTACTGTTAGCATGTTGTAA+TGG + chr1_3:6266149-6266168 Msa0099910:three_prime_UTR 35.0%
TGGTCACAAATGATCTCATT+AGG + chr1_3:6266169-6266188 Msa0099910:three_prime_UTR 35.0%
!!! GAGGAGAATTTTTTTAGCAG+TGG + chr1_3:6266412-6266431 Msa0099910:three_prime_UTR 35.0%
!!! GGAGAATTTTTTTAGCAGTG+GGG + chr1_3:6266414-6266433 Msa0099910:three_prime_UTR 35.0%
TTGTATACCTGCTTCACAAT+GGG - chr1_3:6266513-6266532 None:intergenic 35.0%
TTTGTATACCTGCTTCACAA+TGG - chr1_3:6266514-6266533 None:intergenic 35.0%
! GATAAAGGAAAGGGTGTGAT+GGG + chr1_3:6263223-6263242 Msa0099910:CDS 40.0%
! CATCTTTCTTTTGGCGACTA+AGG - chr1_3:6263357-6263376 None:intergenic 40.0%
GCATACCTGAGATTTCCAAT+TGG - chr1_3:6263386-6263405 None:intergenic 40.0%
ATCAGTTGAGATGTGAGATG+TGG + chr1_3:6263604-6263623 Msa0099910:intron 40.0%
TCAGTTGAGATGTGAGATGT+GGG + chr1_3:6263605-6263624 Msa0099910:intron 40.0%
! ACACTCTGTAAGGCACTTAT+GGG - chr1_3:6263957-6263976 None:intergenic 40.0%
! AACACTCTGTAAGGCACTTA+TGG - chr1_3:6263958-6263977 None:intergenic 40.0%
ATAAGTGCCTTACAGAGTGT+TGG + chr1_3:6263957-6263976 Msa0099910:CDS 40.0%
! ATCCCAGTTCTCTTCTCTTT+TGG + chr1_3:6264554-6264573 Msa0099910:intron 40.0%
ATCCACAGTTCCAATTGCAA+AGG - chr1_3:6264719-6264738 None:intergenic 40.0%
ATGCTAAAGTGGAGCTTCTT+GGG + chr1_3:6264864-6264883 Msa0099910:CDS 40.0%
GGTATTCATCGCCCTTAATT+TGG + chr1_3:6264895-6264914 Msa0099910:CDS 40.0%
GTAACATGCCTCCCAAATTA+AGG - chr1_3:6264910-6264929 None:intergenic 40.0%
! AATTTGGGAGGCATGTTACT+AGG + chr1_3:6264911-6264930 Msa0099910:CDS 40.0%
CATGTTACTAGGTGTTTGGA+AGG + chr1_3:6264922-6264941 Msa0099910:CDS 40.0%
AGGCTGATAAATTCACTGCT+AGG - chr1_3:6265079-6265098 None:intergenic 40.0%
GAGGCAGTAACATGAATAAC+AGG - chr1_3:6265287-6265306 None:intergenic 40.0%
AATGAATTGAGCTGCAGAAG+AGG - chr1_3:6265306-6265325 None:intergenic 40.0%
TCTTCTGCAGCTCAATTCAT+TGG + chr1_3:6265305-6265324 Msa0099910:intron 40.0%
CTTCTGCAGCTCAATTCATT+GGG + chr1_3:6265306-6265325 Msa0099910:intron 40.0%
AAAGAAAATCCAACCTGAGC+AGG - chr1_3:6265375-6265394 None:intergenic 40.0%
AAGCATGTAATCATTGCCTC+TGG - chr1_3:6265629-6265648 None:intergenic 40.0%
CAGCTCATGGAAATCACAAA+AGG - chr1_3:6265981-6266000 None:intergenic 40.0%
! CTTAAGAACTGGAGCATCAA+CGG - chr1_3:6266094-6266113 None:intergenic 40.0%
! TCTTTGGTGACTGCAAGTAT+TGG - chr1_3:6266227-6266246 None:intergenic 40.0%
CAGTCACCAAAGATTAGACA+TGG + chr1_3:6266234-6266253 Msa0099910:three_prime_UTR 40.0%
CATACTAGAAATGTTAGGGG+AGG + chr1_3:6266393-6266412 Msa0099910:three_prime_UTR 40.0%
! GGATAAAGGAAAGGGTGTGA+TGG + chr1_3:6263222-6263241 Msa0099910:CDS 45.0%
GGTAGAATCGTCTTGATCGA+GGG - chr1_3:6263335-6263354 None:intergenic 45.0%
TCTCAACTCATGATGCCGAA+CGG + chr1_3:6263672-6263691 Msa0099910:intron 45.0%
ATCGGTCAATCATTCCCGTT+CGG - chr1_3:6263690-6263709 None:intergenic 45.0%
GATGGGGTTTATGATGTGGA+TGG + chr1_3:6263875-6263894 Msa0099910:CDS 45.0%
GTATCACCTTCTCAGCTCTT+TGG + chr1_3:6263928-6263947 Msa0099910:CDS 45.0%
CTAGGTGTGTGAGTTTCAAG+TGG - chr1_3:6264056-6264075 None:intergenic 45.0%
CACTTGAAACTCACACACCT+AGG + chr1_3:6264054-6264073 Msa0099910:intron 45.0%
ATCTTCACCTGAGATCTCCT+AGG - chr1_3:6264074-6264093 None:intergenic 45.0%
GACCTTTGCAATTGGAACTG+TGG + chr1_3:6264714-6264733 Msa0099910:intron 45.0%
GATGCTAAAGTGGAGCTTCT+TGG + chr1_3:6264863-6264882 Msa0099910:CDS 45.0%
AGGGCGATGAATACCAACTT+AGG - chr1_3:6264890-6264909 None:intergenic 45.0%
! TTCATCGCCCTTAATTTGGG+AGG + chr1_3:6264899-6264918 Msa0099910:CDS 45.0%
GAGGCATGTTACTAGGTGTT+TGG + chr1_3:6264918-6264937 Msa0099910:CDS 45.0%
GCAACAATATCTGTAGACCC+AGG + chr1_3:6265588-6265607 Msa0099910:intron 45.0%
CTTCACAATGGGAAGGTTAC+AGG - chr1_3:6266502-6266521 None:intergenic 45.0%
TAACCTTCCCATTGTGAAGC+AGG + chr1_3:6266503-6266522 Msa0099910:three_prime_UTR 45.0%
ATACCTGCTTCACAATGGGA+AGG - chr1_3:6266509-6266528 None:intergenic 45.0%
! AAGGGTGTGATGGGATCTTC+TGG + chr1_3:6263232-6263251 Msa0099910:CDS 50.0%
TGGGATCTTCTGGACGAAGA+TGG + chr1_3:6263242-6263261 Msa0099910:CDS 50.0%
GGGATCTTCTGGACGAAGAT+GGG + chr1_3:6263243-6263262 Msa0099910:CDS 50.0%
CGCGATATTATCGATCCTCC+CGG + chr1_3:6263295-6263314 Msa0099910:CDS 50.0%
GGGTAGAATCGTCTTGATCG+AGG - chr1_3:6263336-6263355 None:intergenic 50.0%
CTCAACTCATGATGCCGAAC+GGG + chr1_3:6263673-6263692 Msa0099910:intron 50.0%
GAAAGCTTGGGAAGTTGCAC+AGG + chr1_3:6263830-6263849 Msa0099910:intron 50.0%
GGGTTTATGATGTGGATGGC+TGG + chr1_3:6263879-6263898 Msa0099910:CDS 50.0%
TCACACACCTAGGAGATCTC+AGG + chr1_3:6264064-6264083 Msa0099910:intron 50.0%
TTCCTACCCACACATCAGAC+TGG + chr1_3:6265334-6265353 Msa0099910:CDS 50.0%
TCCTACCCACACATCAGACT+GGG + chr1_3:6265335-6265354 Msa0099910:CDS 50.0%
TGACACCCAGTCTGATGTGT+GGG - chr1_3:6265343-6265362 None:intergenic 50.0%
ATGACACCCAGTCTGATGTG+TGG - chr1_3:6265344-6265363 None:intergenic 50.0%
GGGTTGCCAAAGAGCTGAGA+AGG - chr1_3:6263937-6263956 None:intergenic 55.0%
GGAGCTTCTTGGGCCTAAGT+TGG + chr1_3:6264874-6264893 Msa0099910:CDS 55.0%
ACCCAGTCTGATGTGTGGGT+AGG - chr1_3:6265339-6265358 None:intergenic 55.0%
GTCATCCTTAGCACCTGCTC+AGG + chr1_3:6265359-6265378 Msa0099910:CDS 55.0%
TCCTTAGCACCTGCTCAGGT+TGG + chr1_3:6265363-6265382 Msa0099910:intron 55.0%
TCCAACCTGAGCAGGTGCTA+AGG - chr1_3:6265367-6265386 None:intergenic 55.0%
TCCATGAGCTGCAACACCCT+TGG + chr1_3:6265990-6266009 Msa0099910:intron 55.0%
!! ACCAAGGGTGTTGCAGCTCA+TGG - chr1_3:6265994-6266013 None:intergenic 55.0%
! CTTCAGGAGGTGGAGTACTC+AGG + chr1_3:6266053-6266072 Msa0099910:intron 55.0%
! AGGAGGTGGAGTACTCAGGT+GGG + chr1_3:6266057-6266076 Msa0099910:intron 55.0%
! AGGGAAGCGCGTGAGAAACC+GGG - chr1_3:6263316-6263335 None:intergenic 60.0%
! GAGGGAAGCGCGTGAGAAAC+CGG - chr1_3:6263317-6263336 None:intergenic 60.0%
! CAGGAGGTGGAGTACTCAGG+TGG + chr1_3:6266056-6266075 Msa0099910:intron 60.0%
! GGAGGTGGAGTACTCAGGTG+GGG + chr1_3:6266058-6266077 Msa0099910:intron 60.0%
! GAGGTGGAGTACTCAGGTGG+GGG + chr1_3:6266059-6266078 Msa0099910:CDS 60.0%
GAAGCGCGTGAGAAACCGGG+AGG - chr1_3:6263313-6263332 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 6263119 6266544 6263119 ID=Msa0099910;Name=Msa0099910
chr1_3 mRNA 6263119 6266544 6263119 ID=Msa0099910-mRNA-1;Parent=Msa0099910;Name=Msa0099910-mRNA-1;_AED=0.06;_eAED=0.06;_QI=101|1|1|1|1|1|5|447|178
chr1_3 exon 6263119 6263399 6263119 ID=Msa0099910-mRNA-1:exon:2343;Parent=Msa0099910-mRNA-1
chr1_3 exon 6263831 6263984 6263831 ID=Msa0099910-mRNA-1:exon:2344;Parent=Msa0099910-mRNA-1
chr1_3 exon 6264846 6264943 6264846 ID=Msa0099910-mRNA-1:exon:2345;Parent=Msa0099910-mRNA-1
chr1_3 exon 6265315 6265380 6265315 ID=Msa0099910-mRNA-1:exon:2346;Parent=Msa0099910-mRNA-1
chr1_3 exon 6266059 6266544 6266059 ID=Msa0099910-mRNA-1:exon:2347;Parent=Msa0099910-mRNA-1
chr1_3 five_prime_UTR 6263119 6263219 6263119 ID=Msa0099910-mRNA-1:five_prime_utr;Parent=Msa0099910-mRNA-1
chr1_3 CDS 6263220 6263399 6263220 ID=Msa0099910-mRNA-1:cds;Parent=Msa0099910-mRNA-1
chr1_3 CDS 6263831 6263984 6263831 ID=Msa0099910-mRNA-1:cds;Parent=Msa0099910-mRNA-1
chr1_3 CDS 6264846 6264943 6264846 ID=Msa0099910-mRNA-1:cds;Parent=Msa0099910-mRNA-1
chr1_3 CDS 6265315 6265380 6265315 ID=Msa0099910-mRNA-1:cds;Parent=Msa0099910-mRNA-1
chr1_3 CDS 6266059 6266097 6266059 ID=Msa0099910-mRNA-1:cds;Parent=Msa0099910-mRNA-1
chr1_3 three_prime_UTR 6266098 6266544 6266098 ID=Msa0099910-mRNA-1:three_prime_utr;Parent=Msa0099910-mRNA-1
Gene Sequence

>Msa0099910

ATGGATAAAGGAAAGGGTGTGATGGGATCTTCTGGACGAAGATGGGCCGTTGATTTCTCCGATAATTCAACTTCTCGCGATATTATCGATCCTCCCGGTTTCTCACGCGCTTCCCTCGATCAAGACGATTCTACCCTTAGTCGCCAAAAGAAAGATGCTGAATCCAATTGGAAATCTCAGAAAGCTTGGGAAGTTGCACAGGCACCATTTAAGAACTTGTTAATGATGGGGTTTATGATGTGGATGGCTGGTAATACTGTTCACTTGTTTAGCATCGGTATCACCTTCTCAGCTCTTTGGCAACCCATAAGTGCCTTACAGAGTGTTGGAAAAATGTTTGAACCTTACAAAGATGCTAAAGTGGAGCTTCTTGGGCCTAAGTTGGTATTCATCGCCCTTAATTTGGGAGGCATGTTACTAGGTGTTTGGAAGCTCAATTCATTGGGACTGCTTCCTACCCACACATCAGACTGGGTGTCATCCTTAGCACCTGCTCAGGAGGTGGAGTACTCAGGTGGGGGTCTAAATTTCCGTTGA
Protein sequence

>Msa0099910

MDKGKGVMGSSGRRWAVDFSDNSTSRDIIDPPGFSRASLDQDDSTLSRQKKDAESNWKSQKAWEVAQAPFKNLLMMGFMMWMAGNTVHLFSIGITFSALWQPISALQSVGKMFEPYKDAKVELLGPKLVFIALNLGGMLLGVWKLNSLGLLPTHTSDWVSSLAPAQEVEYSGGGLNFR*