AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0364060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0364060 MtrunA17_Chr3g0103371 94.631 149 7 1 1 148 1 149 5.20e-89 255
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0364060 AT2G01870.1 42.453 106 45 5 50 148 30 126 1.08e-15 70.1

Find 43 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATGAAGATAATGTTTA+TGG 0.128032 3_1:-56743434 None:intergenic
AAGCTCTTTGAGGGCTTCTT+TGG 0.197880 3_1:-56743728 None:intergenic
AGGGCTTCTTTGGCTTTCTC+TGG 0.231668 3_1:-56743718 None:intergenic
TCAACTTAGGAAAGAATATT+TGG 0.243444 3_1:+56745306 Msa0364060:three_prime_UTR
CTGCATAGTTCATGCATTAA+AGG 0.282045 3_1:+56743669 Msa0364060:CDS
GTGTGATATTAATTAATTTA+CGG 0.287568 3_1:+56745167 Msa0364060:three_prime_UTR
GAGAGAGTACCCTTGAGTTT+TGG 0.292782 3_1:-56743793 None:intergenic
GAGAGTGATGCAAATAATAA+TGG 0.393343 3_1:-56743568 None:intergenic
GTAATTAAGCCAGCCATTGA+TGG 0.399846 3_1:+56745119 Msa0364060:CDS
CTGCACTTGGATATAGGTTA+TGG 0.412024 3_1:-56745143 None:intergenic
TCGCTACATATATAAAGTTA+AGG 0.412406 3_1:+56745195 Msa0364060:three_prime_UTR
ACATATATAAAGTTAAGGTT+TGG 0.420592 3_1:+56745200 Msa0364060:three_prime_UTR
TTGGGTGAGTTTAGTGTAGA+GGG 0.423780 3_1:+56743479 Msa0364060:five_prime_UTR
CGTTTATCATTATGTAATAA+CGG 0.426588 3_1:-56743595 None:intergenic
TTTCTGGTCAAGCTCTTTGA+GGG 0.426726 3_1:-56743737 None:intergenic
CACACACTGCACTTGGATAT+AGG 0.429892 3_1:-56745149 None:intergenic
GGGCTTCTTTGGCTTTCTCT+GGG 0.431656 3_1:-56743717 None:intergenic
TCCTTTCTTGCACTACTAGC+TGG 0.435956 3_1:+56745050 Msa0364060:CDS
ACAATTGATGAAAATTTAAG+TGG 0.457103 3_1:-56745331 None:intergenic
TTAATATCACACACTGCACT+TGG 0.458769 3_1:-56745156 None:intergenic
ACCAGCTAGTAGTGCAAGAA+AGG 0.484237 3_1:-56745051 None:intergenic
CAGCATGAATTAAATGAAGA+AGG 0.488178 3_1:-56743649 None:intergenic
GGCTGCTTATAGATGCTAAG+AGG 0.520680 3_1:-56743544 None:intergenic
ATAAACGCATATTCAAAGAA+AGG 0.527079 3_1:+56743611 Msa0364060:CDS
TATAGGTTATGGACCATCAA+TGG 0.534653 3_1:-56745132 None:intergenic
GACAACATTAAAATCAACTT+AGG 0.546871 3_1:+56745293 Msa0364060:three_prime_UTR
TTTGGGTGAGTTTAGTGTAG+AGG 0.549195 3_1:+56743478 Msa0364060:five_prime_UTR
TGGCTGGCTTAATTACAGTA+AGG 0.551216 3_1:-56745112 None:intergenic
TTTCTTGCACTACTAGCTGG+TGG 0.557537 3_1:+56745053 Msa0364060:CDS
AGTTTCCTCTCCAAAACTCA+AGG 0.567427 3_1:+56743783 Msa0364060:CDS
GGTTATGGACCATCAATGGC+TGG 0.567707 3_1:-56745128 None:intergenic
GTGAGTTTAGTGTAGAGGGA+AGG 0.576557 3_1:+56743483 Msa0364060:five_prime_UTR
CTTATCACCTCCTCTCTCGT+AGG 0.590439 3_1:-56744996 None:intergenic
GTTTCCTCTCCAAAACTCAA+GGG 0.619477 3_1:+56743784 Msa0364060:CDS
GAATAACATCTGAATATGAG+TGG 0.621057 3_1:-56743512 None:intergenic
AGAGATGAAGCCTACGAGAG+AGG 0.636132 3_1:+56744986 Msa0364060:CDS
CAGAATCTTAGCAACATGCG+TGG 0.639323 3_1:-56745268 None:intergenic
AGAATCTTAGCAACATGCGT+GGG 0.645379 3_1:-56745267 None:intergenic
AACGCATATTCAAAGAAAGG+AGG 0.648340 3_1:+56743614 Msa0364060:CDS
CAATTGATGAAAATTTAAGT+GGG 0.656477 3_1:-56745330 None:intergenic
GATGAAGCCTACGAGAGAGG+AGG 0.660242 3_1:+56744989 Msa0364060:CDS
AGTGATGCAAATAATAATGG+TGG 0.683648 3_1:-56743565 None:intergenic
GAGGTGATAAGTGACAACAA+TGG 0.780630 3_1:+56745008 Msa0364060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAGAGAAAGAAA+CGG - chr3_1:56743818-56743837 None:intergenic 15.0%
!!! ATTTTCCTTTTTTAATTGTA+TGG + chr3_1:56743946-56743965 Msa0364060:intron 15.0%
!!! TATAGCTTTGATAAAAAAAA+AGG - chr3_1:56744453-56744472 None:intergenic 15.0%
!! ATTATTCAACATATTTGAAT+CGG - chr3_1:56744493-56744512 None:intergenic 15.0%
!!! AAACTAAAAGTATTTATAAG+TGG - chr3_1:56744681-56744700 None:intergenic 15.0%
!! GTGTGATATTAATTAATTTA+CGG + chr3_1:56745167-56745186 Msa0364060:three_prime_UTR 15.0%
!! AAAAAAAAAAGAGAAAGAAA+CGG - chr3_1:56743818-56743837 None:intergenic 15.0%
!!! ATTTTCCTTTTTTAATTGTA+TGG + chr3_1:56743946-56743965 Msa0364060:intron 15.0%
!!! TATAGCTTTGATAAAAAAAA+AGG - chr3_1:56744453-56744472 None:intergenic 15.0%
!! ATTATTCAACATATTTGAAT+CGG - chr3_1:56744493-56744512 None:intergenic 15.0%
!!! AAACTAAAAGTATTTATAAG+TGG - chr3_1:56744681-56744700 None:intergenic 15.0%
!! GTGTGATATTAATTAATTTA+CGG + chr3_1:56745167-56745186 Msa0364060:three_prime_UTR 15.0%
!! CGTTTATCATTATGTAATAA+CGG - chr3_1:56743598-56743617 None:intergenic 20.0%
!! CAAAACCATACAATTAAAAA+AGG - chr3_1:56743954-56743973 None:intergenic 20.0%
!!! TGGTTTTGTTTATTTCTTTT+TGG + chr3_1:56743966-56743985 Msa0364060:intron 20.0%
!! TCAAAGACTAAACTAATTAA+CGG - chr3_1:56744085-56744104 None:intergenic 20.0%
!!! ATGTGGGAAAATTTTTTTTT+TGG + chr3_1:56744225-56744244 Msa0364060:intron 20.0%
!! CGAAATGCAAATTTATAAAA+GGG - chr3_1:56744362-56744381 None:intergenic 20.0%
!! ACGAAATGCAAATTTATAAA+AGG - chr3_1:56744363-56744382 None:intergenic 20.0%
!! ATAACAACTTGTATAATCTT+AGG - chr3_1:56744397-56744416 None:intergenic 20.0%
!!! ACATCATATATAGCTAATTT+TGG - chr3_1:56744421-56744440 None:intergenic 20.0%
!!! ATATATGAGATTTGAACTTT+TGG - chr3_1:56744630-56744649 None:intergenic 20.0%
!! GGAAATTAAAAAGTTTATGT+AGG - chr3_1:56744715-56744734 None:intergenic 20.0%
!! ACATATATAAAGTTAAGGTT+TGG + chr3_1:56745200-56745219 Msa0364060:three_prime_UTR 20.0%
!! CAATTGATGAAAATTTAAGT+GGG - chr3_1:56745333-56745352 None:intergenic 20.0%
!! ACAATTGATGAAAATTTAAG+TGG - chr3_1:56745334-56745353 None:intergenic 20.0%
!! CGTTTATCATTATGTAATAA+CGG - chr3_1:56743598-56743617 None:intergenic 20.0%
!! CAAAACCATACAATTAAAAA+AGG - chr3_1:56743954-56743973 None:intergenic 20.0%
!!! TGGTTTTGTTTATTTCTTTT+TGG + chr3_1:56743966-56743985 Msa0364060:intron 20.0%
!! TCAAAGACTAAACTAATTAA+CGG - chr3_1:56744085-56744104 None:intergenic 20.0%
!!! ATGTGGGAAAATTTTTTTTT+TGG + chr3_1:56744225-56744244 Msa0364060:intron 20.0%
!! CGAAATGCAAATTTATAAAA+GGG - chr3_1:56744362-56744381 None:intergenic 20.0%
!! ACGAAATGCAAATTTATAAA+AGG - chr3_1:56744363-56744382 None:intergenic 20.0%
!! ATAACAACTTGTATAATCTT+AGG - chr3_1:56744397-56744416 None:intergenic 20.0%
!!! ACATCATATATAGCTAATTT+TGG - chr3_1:56744421-56744440 None:intergenic 20.0%
!!! ATATATGAGATTTGAACTTT+TGG - chr3_1:56744630-56744649 None:intergenic 20.0%
!! GGAAATTAAAAAGTTTATGT+AGG - chr3_1:56744715-56744734 None:intergenic 20.0%
!! ACATATATAAAGTTAAGGTT+TGG + chr3_1:56745200-56745219 Msa0364060:three_prime_UTR 20.0%
!! CAATTGATGAAAATTTAAGT+GGG - chr3_1:56745333-56745352 None:intergenic 20.0%
!! ACAATTGATGAAAATTTAAG+TGG - chr3_1:56745334-56745353 None:intergenic 20.0%
! GGAAATGAAGATAATGTTTA+TGG - chr3_1:56743437-56743456 None:intergenic 25.0%
! ATAAACGCATATTCAAAGAA+AGG + chr3_1:56743611-56743630 Msa0364060:CDS 25.0%
!! TGTAGTGCTTTTAAATTTTG+TGG + chr3_1:56743851-56743870 Msa0364060:intron 25.0%
! GAATTGCAATTGATTAGATT+AGG + chr3_1:56743921-56743940 Msa0364060:intron 25.0%
!! TTGCATTTTATCTTGAGTTA+GGG - chr3_1:56744040-56744059 None:intergenic 25.0%
!! TTTGCATTTTATCTTGAGTT+AGG - chr3_1:56744041-56744060 None:intergenic 25.0%
! CAAAGACTAAACTAATTAAC+GGG - chr3_1:56744084-56744103 None:intergenic 25.0%
!!! TTCTCTTCATTTTTCATTCT+AGG + chr3_1:56744536-56744555 Msa0364060:intron 25.0%
! ATATATGAACTCAAACTCTT+TGG + chr3_1:56744646-56744665 Msa0364060:intron 25.0%
! TGTAAGGACATGTTAAAAAT+AGG + chr3_1:56744746-56744765 Msa0364060:intron 25.0%
! TCGCTACATATATAAAGTTA+AGG + chr3_1:56745195-56745214 Msa0364060:three_prime_UTR 25.0%
! GACAACATTAAAATCAACTT+AGG + chr3_1:56745293-56745312 Msa0364060:three_prime_UTR 25.0%
! TCAACTTAGGAAAGAATATT+TGG + chr3_1:56745306-56745325 Msa0364060:three_prime_UTR 25.0%
! GGAAATGAAGATAATGTTTA+TGG - chr3_1:56743437-56743456 None:intergenic 25.0%
! ATAAACGCATATTCAAAGAA+AGG + chr3_1:56743611-56743630 Msa0364060:CDS 25.0%
!! TGTAGTGCTTTTAAATTTTG+TGG + chr3_1:56743851-56743870 Msa0364060:intron 25.0%
! GAATTGCAATTGATTAGATT+AGG + chr3_1:56743921-56743940 Msa0364060:intron 25.0%
!! TTGCATTTTATCTTGAGTTA+GGG - chr3_1:56744040-56744059 None:intergenic 25.0%
!! TTTGCATTTTATCTTGAGTT+AGG - chr3_1:56744041-56744060 None:intergenic 25.0%
! CAAAGACTAAACTAATTAAC+GGG - chr3_1:56744084-56744103 None:intergenic 25.0%
!!! TTCTCTTCATTTTTCATTCT+AGG + chr3_1:56744536-56744555 Msa0364060:intron 25.0%
! ATATATGAACTCAAACTCTT+TGG + chr3_1:56744646-56744665 Msa0364060:intron 25.0%
! TGTAAGGACATGTTAAAAAT+AGG + chr3_1:56744746-56744765 Msa0364060:intron 25.0%
! TCGCTACATATATAAAGTTA+AGG + chr3_1:56745195-56745214 Msa0364060:three_prime_UTR 25.0%
! GACAACATTAAAATCAACTT+AGG + chr3_1:56745293-56745312 Msa0364060:three_prime_UTR 25.0%
! TCAACTTAGGAAAGAATATT+TGG + chr3_1:56745306-56745325 Msa0364060:three_prime_UTR 25.0%
CTTCTCGTGTCTAAAAATTT+GGG + chr3_1:56743461-56743480 Msa0364060:five_prime_UTR 30.0%
GAATAACATCTGAATATGAG+TGG - chr3_1:56743515-56743534 None:intergenic 30.0%
AGTGATGCAAATAATAATGG+TGG - chr3_1:56743568-56743587 None:intergenic 30.0%
GAGAGTGATGCAAATAATAA+TGG - chr3_1:56743571-56743590 None:intergenic 30.0%
CAGCATGAATTAAATGAAGA+AGG - chr3_1:56743652-56743671 None:intergenic 30.0%
! TAGAGACAGATTGAATTTTC+TGG - chr3_1:56743756-56743775 None:intergenic 30.0%
!!! AATTTTTTTTTTGGCACCGA+AGG + chr3_1:56744234-56744253 Msa0364060:intron 30.0%
ATTACCATTCTTGAGACTTT+CGG + chr3_1:56744258-56744277 Msa0364060:intron 30.0%
TTTCCAACGATTACTATGTA+AGG + chr3_1:56744730-56744749 Msa0364060:intron 30.0%
! ATAGTAACATTTTGTAGCAC+AGG + chr3_1:56744854-56744873 Msa0364060:intron 30.0%
CTTCTCGTGTCTAAAAATTT+GGG + chr3_1:56743461-56743480 Msa0364060:five_prime_UTR 30.0%
GAATAACATCTGAATATGAG+TGG - chr3_1:56743515-56743534 None:intergenic 30.0%
AGTGATGCAAATAATAATGG+TGG - chr3_1:56743568-56743587 None:intergenic 30.0%
GAGAGTGATGCAAATAATAA+TGG - chr3_1:56743571-56743590 None:intergenic 30.0%
CAGCATGAATTAAATGAAGA+AGG - chr3_1:56743652-56743671 None:intergenic 30.0%
! TAGAGACAGATTGAATTTTC+TGG - chr3_1:56743756-56743775 None:intergenic 30.0%
!!! AATTTTTTTTTTGGCACCGA+AGG + chr3_1:56744234-56744253 Msa0364060:intron 30.0%
ATTACCATTCTTGAGACTTT+CGG + chr3_1:56744258-56744277 Msa0364060:intron 30.0%
TTTCCAACGATTACTATGTA+AGG + chr3_1:56744730-56744749 Msa0364060:intron 30.0%
! ATAGTAACATTTTGTAGCAC+AGG + chr3_1:56744854-56744873 Msa0364060:intron 30.0%
! TTTTTAGACACGAGAAGCAA+TGG - chr3_1:56743458-56743477 None:intergenic 35.0%
GCTTCTCGTGTCTAAAAATT+TGG + chr3_1:56743460-56743479 Msa0364060:five_prime_UTR 35.0%
AACGCATATTCAAAGAAAGG+AGG + chr3_1:56743614-56743633 Msa0364060:CDS 35.0%
CTGCATAGTTCATGCATTAA+AGG + chr3_1:56743669-56743688 Msa0364060:CDS 35.0%
AACAGCAATCCCTTAATTGT+AGG + chr3_1:56744186-56744205 Msa0364060:intron 35.0%
CTCAAGAATGGTAATTCCTT+CGG - chr3_1:56744253-56744272 None:intergenic 35.0%
TTACCATTCTTGAGACTTTC+GGG + chr3_1:56744259-56744278 Msa0364060:intron 35.0%
TGTCCTTACATAGTAATCGT+TGG - chr3_1:56744736-56744755 None:intergenic 35.0%
TTAGACGAACATTTATCCTC+AGG - chr3_1:56744800-56744819 None:intergenic 35.0%
TATAGGTTATGGACCATCAA+TGG - chr3_1:56745135-56745154 None:intergenic 35.0%
TTAATATCACACACTGCACT+TGG - chr3_1:56745159-56745178 None:intergenic 35.0%
ATGCGTGGGATATAAAAACA+TGG - chr3_1:56745256-56745275 None:intergenic 35.0%
! TTTTTAGACACGAGAAGCAA+TGG - chr3_1:56743458-56743477 None:intergenic 35.0%
GCTTCTCGTGTCTAAAAATT+TGG + chr3_1:56743460-56743479 Msa0364060:five_prime_UTR 35.0%
AACGCATATTCAAAGAAAGG+AGG + chr3_1:56743614-56743633 Msa0364060:CDS 35.0%
CTGCATAGTTCATGCATTAA+AGG + chr3_1:56743669-56743688 Msa0364060:CDS 35.0%
AACAGCAATCCCTTAATTGT+AGG + chr3_1:56744186-56744205 Msa0364060:intron 35.0%
CTCAAGAATGGTAATTCCTT+CGG - chr3_1:56744253-56744272 None:intergenic 35.0%
TTACCATTCTTGAGACTTTC+GGG + chr3_1:56744259-56744278 Msa0364060:intron 35.0%
TGTCCTTACATAGTAATCGT+TGG - chr3_1:56744736-56744755 None:intergenic 35.0%
TTAGACGAACATTTATCCTC+AGG - chr3_1:56744800-56744819 None:intergenic 35.0%
TATAGGTTATGGACCATCAA+TGG - chr3_1:56745135-56745154 None:intergenic 35.0%
TTAATATCACACACTGCACT+TGG - chr3_1:56745159-56745178 None:intergenic 35.0%
ATGCGTGGGATATAAAAACA+TGG - chr3_1:56745256-56745275 None:intergenic 35.0%
TTTGGGTGAGTTTAGTGTAG+AGG + chr3_1:56743478-56743497 Msa0364060:five_prime_UTR 40.0%
TTGGGTGAGTTTAGTGTAGA+GGG + chr3_1:56743479-56743498 Msa0364060:five_prime_UTR 40.0%
! TTTCTGGTCAAGCTCTTTGA+GGG - chr3_1:56743740-56743759 None:intergenic 40.0%
!! TTTTCTGGTCAAGCTCTTTG+AGG - chr3_1:56743741-56743760 None:intergenic 40.0%
AGTTTCCTCTCCAAAACTCA+AGG + chr3_1:56743783-56743802 Msa0364060:CDS 40.0%
GTTTCCTCTCCAAAACTCAA+GGG + chr3_1:56743784-56743803 Msa0364060:CDS 40.0%
! AGACGAATGATTTTGTGAGC+AGG + chr3_1:56743896-56743915 Msa0364060:intron 40.0%
!!! TGCTGTTGTTTTTAGTGCGT+CGG - chr3_1:56744173-56744192 None:intergenic 40.0%
! AATTTTCCCACATGCATGCT+GGG - chr3_1:56744218-56744237 None:intergenic 40.0%
! AAATTTTCCCACATGCATGC+TGG - chr3_1:56744219-56744238 None:intergenic 40.0%
TATCCCGAAAGTCTCAAGAA+TGG - chr3_1:56744265-56744284 None:intergenic 40.0%
!! AGTAACTTCTGAAGTACCTG+AGG + chr3_1:56744781-56744800 Msa0364060:intron 40.0%
GAGGTGATAAGTGACAACAA+TGG + chr3_1:56745008-56745027 Msa0364060:CDS 40.0%
!! TGGCTGGCTTAATTACAGTA+AGG - chr3_1:56745115-56745134 None:intergenic 40.0%
GTAATTAAGCCAGCCATTGA+TGG + chr3_1:56745119-56745138 Msa0364060:CDS 40.0%
CTGCACTTGGATATAGGTTA+TGG - chr3_1:56745146-56745165 None:intergenic 40.0%
AGAATCTTAGCAACATGCGT+GGG - chr3_1:56745270-56745289 None:intergenic 40.0%
TTTGGGTGAGTTTAGTGTAG+AGG + chr3_1:56743478-56743497 Msa0364060:five_prime_UTR 40.0%
TTGGGTGAGTTTAGTGTAGA+GGG + chr3_1:56743479-56743498 Msa0364060:five_prime_UTR 40.0%
! TTTCTGGTCAAGCTCTTTGA+GGG - chr3_1:56743740-56743759 None:intergenic 40.0%
!! TTTTCTGGTCAAGCTCTTTG+AGG - chr3_1:56743741-56743760 None:intergenic 40.0%
AGTTTCCTCTCCAAAACTCA+AGG + chr3_1:56743783-56743802 Msa0364060:CDS 40.0%
GTTTCCTCTCCAAAACTCAA+GGG + chr3_1:56743784-56743803 Msa0364060:CDS 40.0%
! AGACGAATGATTTTGTGAGC+AGG + chr3_1:56743896-56743915 Msa0364060:intron 40.0%
!!! TGCTGTTGTTTTTAGTGCGT+CGG - chr3_1:56744173-56744192 None:intergenic 40.0%
! AATTTTCCCACATGCATGCT+GGG - chr3_1:56744218-56744237 None:intergenic 40.0%
! AAATTTTCCCACATGCATGC+TGG - chr3_1:56744219-56744238 None:intergenic 40.0%
TATCCCGAAAGTCTCAAGAA+TGG - chr3_1:56744265-56744284 None:intergenic 40.0%
!! AGTAACTTCTGAAGTACCTG+AGG + chr3_1:56744781-56744800 Msa0364060:intron 40.0%
GAGGTGATAAGTGACAACAA+TGG + chr3_1:56745008-56745027 Msa0364060:CDS 40.0%
!! TGGCTGGCTTAATTACAGTA+AGG - chr3_1:56745115-56745134 None:intergenic 40.0%
GTAATTAAGCCAGCCATTGA+TGG + chr3_1:56745119-56745138 Msa0364060:CDS 40.0%
CTGCACTTGGATATAGGTTA+TGG - chr3_1:56745146-56745165 None:intergenic 40.0%
AGAATCTTAGCAACATGCGT+GGG - chr3_1:56745270-56745289 None:intergenic 40.0%
GTGAGTTTAGTGTAGAGGGA+AGG + chr3_1:56743483-56743502 Msa0364060:five_prime_UTR 45.0%
GGCTGCTTATAGATGCTAAG+AGG - chr3_1:56743547-56743566 None:intergenic 45.0%
! AAGCTCTTTGAGGGCTTCTT+TGG - chr3_1:56743731-56743750 None:intergenic 45.0%
!!! AGTACCCTTGAGTTTTGGAG+AGG - chr3_1:56743791-56743810 None:intergenic 45.0%
!!! GAGAGAGTACCCTTGAGTTT+TGG - chr3_1:56743796-56743815 None:intergenic 45.0%
!!! GCTGTTGTTTTTAGTGCGTC+GGG - chr3_1:56744172-56744191 None:intergenic 45.0%
GCATCACTCCACTGTAATAG+CGG + chr3_1:56744304-56744323 Msa0364060:intron 45.0%
CATCACTCCACTGTAATAGC+GGG + chr3_1:56744305-56744324 Msa0364060:intron 45.0%
ATCACTCCACTGTAATAGCG+GGG + chr3_1:56744306-56744325 Msa0364060:intron 45.0%
TTTACACCCCGCTATTACAG+TGG - chr3_1:56744315-56744334 None:intergenic 45.0%
! AATAGCGGGGTGTAAAGTCA+AGG + chr3_1:56744319-56744338 Msa0364060:intron 45.0%
! ATAGCGGGGTGTAAAGTCAA+GGG + chr3_1:56744320-56744339 Msa0364060:intron 45.0%
TCCTTTCTTGCACTACTAGC+TGG + chr3_1:56745050-56745069 Msa0364060:CDS 45.0%
ACCAGCTAGTAGTGCAAGAA+AGG - chr3_1:56745054-56745073 None:intergenic 45.0%
TTTCTTGCACTACTAGCTGG+TGG + chr3_1:56745053-56745072 Msa0364060:CDS 45.0%
CACACACTGCACTTGGATAT+AGG - chr3_1:56745152-56745171 None:intergenic 45.0%
CAGAATCTTAGCAACATGCG+TGG - chr3_1:56745271-56745290 None:intergenic 45.0%
GTGAGTTTAGTGTAGAGGGA+AGG + chr3_1:56743483-56743502 Msa0364060:five_prime_UTR 45.0%
GGCTGCTTATAGATGCTAAG+AGG - chr3_1:56743547-56743566 None:intergenic 45.0%
! AAGCTCTTTGAGGGCTTCTT+TGG - chr3_1:56743731-56743750 None:intergenic 45.0%
!!! AGTACCCTTGAGTTTTGGAG+AGG - chr3_1:56743791-56743810 None:intergenic 45.0%
!!! GAGAGAGTACCCTTGAGTTT+TGG - chr3_1:56743796-56743815 None:intergenic 45.0%
!!! GCTGTTGTTTTTAGTGCGTC+GGG - chr3_1:56744172-56744191 None:intergenic 45.0%
GCATCACTCCACTGTAATAG+CGG + chr3_1:56744304-56744323 Msa0364060:intron 45.0%
CATCACTCCACTGTAATAGC+GGG + chr3_1:56744305-56744324 Msa0364060:intron 45.0%
ATCACTCCACTGTAATAGCG+GGG + chr3_1:56744306-56744325 Msa0364060:intron 45.0%
TTTACACCCCGCTATTACAG+TGG - chr3_1:56744315-56744334 None:intergenic 45.0%
! AATAGCGGGGTGTAAAGTCA+AGG + chr3_1:56744319-56744338 Msa0364060:intron 45.0%
! ATAGCGGGGTGTAAAGTCAA+GGG + chr3_1:56744320-56744339 Msa0364060:intron 45.0%
TCCTTTCTTGCACTACTAGC+TGG + chr3_1:56745050-56745069 Msa0364060:CDS 45.0%
ACCAGCTAGTAGTGCAAGAA+AGG - chr3_1:56745054-56745073 None:intergenic 45.0%
TTTCTTGCACTACTAGCTGG+TGG + chr3_1:56745053-56745072 Msa0364060:CDS 45.0%
CACACACTGCACTTGGATAT+AGG - chr3_1:56745152-56745171 None:intergenic 45.0%
CAGAATCTTAGCAACATGCG+TGG - chr3_1:56745271-56745290 None:intergenic 45.0%
!! GGGCTTCTTTGGCTTTCTCT+GGG - chr3_1:56743720-56743739 None:intergenic 50.0%
!! AGGGCTTCTTTGGCTTTCTC+TGG - chr3_1:56743721-56743740 None:intergenic 50.0%
!! TTAGTGCGTCGGGAAGCATT+CGG - chr3_1:56744162-56744181 None:intergenic 50.0%
GGGCGGTTGCCTACAATTAA+GGG - chr3_1:56744198-56744217 None:intergenic 50.0%
TGGGCGGTTGCCTACAATTA+AGG - chr3_1:56744199-56744218 None:intergenic 50.0%
AGAGATGAAGCCTACGAGAG+AGG + chr3_1:56744986-56745005 Msa0364060:CDS 50.0%
CTTATCACCTCCTCTCTCGT+AGG - chr3_1:56744999-56745018 None:intergenic 50.0%
GGTTATGGACCATCAATGGC+TGG - chr3_1:56745131-56745150 None:intergenic 50.0%
!! GGGCTTCTTTGGCTTTCTCT+GGG - chr3_1:56743720-56743739 None:intergenic 50.0%
!! AGGGCTTCTTTGGCTTTCTC+TGG - chr3_1:56743721-56743740 None:intergenic 50.0%
!! TTAGTGCGTCGGGAAGCATT+CGG - chr3_1:56744162-56744181 None:intergenic 50.0%
GGGCGGTTGCCTACAATTAA+GGG - chr3_1:56744198-56744217 None:intergenic 50.0%
TGGGCGGTTGCCTACAATTA+AGG - chr3_1:56744199-56744218 None:intergenic 50.0%
AGAGATGAAGCCTACGAGAG+AGG + chr3_1:56744986-56745005 Msa0364060:CDS 50.0%
CTTATCACCTCCTCTCTCGT+AGG - chr3_1:56744999-56745018 None:intergenic 50.0%
GGTTATGGACCATCAATGGC+TGG - chr3_1:56745131-56745150 None:intergenic 50.0%
!! TAGTGCGTCGGGAAGCATTC+GGG - chr3_1:56744161-56744180 None:intergenic 55.0%
TTTCCCACATGCATGCTGGG+CGG - chr3_1:56744215-56744234 None:intergenic 55.0%
GATGAAGCCTACGAGAGAGG+AGG + chr3_1:56744989-56745008 Msa0364060:CDS 55.0%
!! TAGTGCGTCGGGAAGCATTC+GGG - chr3_1:56744161-56744180 None:intergenic 55.0%
TTTCCCACATGCATGCTGGG+CGG - chr3_1:56744215-56744234 None:intergenic 55.0%
GATGAAGCCTACGAGAGAGG+AGG + chr3_1:56744989-56745008 Msa0364060:CDS 55.0%
CAACCGCCCAGCATGCATGT+GGG + chr3_1:56744209-56744228 Msa0364060:intron 60.0%
CAACCGCCCAGCATGCATGT+GGG + chr3_1:56744209-56744228 Msa0364060:intron 60.0%
GCAACCGCCCAGCATGCATG+TGG + chr3_1:56744208-56744227 Msa0364060:intron 65.0%
GCAACCGCCCAGCATGCATG+TGG + chr3_1:56744208-56744227 Msa0364060:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 56743421 56745381 56743421 ID=Msa0364060;Name=Msa0364060
chr3_1 mRNA 56743421 56745381 56743421 ID=Msa0364060-mRNA-1;Parent=Msa0364060;Name=Msa0364060-mRNA-1;_AED=0.00;_eAED=0.00;_QI=105|1|1|1|1|1|2|233|148
chr3_1 exon 56743421 56743805 56743421 ID=Msa0364060-mRNA-1:exon:10571;Parent=Msa0364060-mRNA-1
chr3_1 exon 56744982 56745381 56744982 ID=Msa0364060-mRNA-1:exon:10572;Parent=Msa0364060-mRNA-1
chr3_1 five_prime_UTR 56743421 56743525 56743421 ID=Msa0364060-mRNA-1:five_prime_utr;Parent=Msa0364060-mRNA-1
chr3_1 CDS 56743526 56743805 56743526 ID=Msa0364060-mRNA-1:cds;Parent=Msa0364060-mRNA-1
chr3_1 CDS 56744982 56745148 56744982 ID=Msa0364060-mRNA-1:cds;Parent=Msa0364060-mRNA-1
chr3_1 three_prime_UTR 56745149 56745381 56745149 ID=Msa0364060-mRNA-1:three_prime_utr;Parent=Msa0364060-mRNA-1
Gene Sequence

>Msa0364060

ATGTTATTCTCTATGAAACCTCTTAGCATCTATAAGCAGCCACCATTATTATTTGCATCACTCTCTAAACCGTTATTACATAATGATAAACGCATATTCAAAGAAAGGAGGCTCATAAAATATCCTTCTTCATTTAATTCATGCTGCATAGTTCATGCATTAAAGGAAGATTCAAAGCAATATGAGATAGACCCAGAGAAAGCCAAAGAAGCCCTCAAAGAGCTTGACCAGAAAATTCAATCTGTCTCTAGTAGAGAAGTTTCCTCTCCAAAACTCAAGGTTTCAGAGATGAAGCCTACGAGAGAGGAGGTGATAAGTGACAACAATGGTAAGCTAGAAATTTCAGAGTCCTTTCTTGCACTACTAGCTGGTGGACTTGTTCTCTTTACTATATTGTACAATGTACTATTCCTTACTGTAATTAAGCCAGCCATTGATGGTCCATAA
Protein sequence

>Msa0364060

MLFSMKPLSIYKQPPLLFASLSKPLLHNDKRIFKERRLIKYPSSFNSCCIVHALKEDSKQYEIDPEKAKEALKELDQKIQSVSSREVSSPKLKVSEMKPTREEVISDNNGKLEISESFLALLAGGLVLFTILYNVLFLTVIKPAIDGP*