AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0371440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0371440 MtrunA17_Chr3g0118211 81.301 123 3 1 1 103 1 123 8.89e-63 189
Msa0371440 MtrunA17_Chr4g0059571 59.055 127 29 2 1 104 1 127 4.14e-43 139
Msa0371440 MtrunA17_Chr1g0170741 51.887 106 28 1 22 104 7 112 2.19e-30 107
Msa0371440 MtrunA17_Chr5g0414931 51.786 112 30 2 17 104 10 121 1.20e-28 103
Msa0371440 MtrunA17_Chr7g0256551 43.103 116 37 2 18 104 4 119 1.66e-24 92.8
Msa0371440 MtrunA17_Chr3g0082861 42.105 114 37 2 20 104 1 114 1.07e-21 84.0
Msa0371440 MtrunA17_Chr8g0352871 40.870 115 40 2 18 104 4 118 1.56e-21 83.6
Msa0371440 MtrunA17_Chr3g0128691 40.385 104 38 2 24 104 19 121 3.27e-21 82.4
Msa0371440 MtrunA17_Chr3g0124861 39.831 118 32 2 23 102 22 138 1.71e-18 77.0
Msa0371440 MtrunA17_Chr4g0021731 51.786 56 27 0 15 70 3 58 9.92e-13 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0371440 AT3G48100.1 53.968 126 32 3 5 104 2 127 1.63e-35 120
Msa0371440 AT1G74890.1 60.194 103 21 1 22 104 17 119 4.43e-35 119
Msa0371440 AT1G19050.1 55.000 120 32 2 5 102 4 123 8.05e-35 119
Msa0371440 AT5G62920.1 58.824 102 22 1 23 104 25 126 1.09e-33 115
Msa0371440 AT1G59940.1 49.265 136 35 4 1 104 1 134 5.51e-32 112
Msa0371440 AT1G59940.2 49.265 136 35 4 1 104 93 226 3.34e-31 112
Msa0371440 AT1G10470.3 53.153 111 32 1 14 104 11 121 4.80e-31 110
Msa0371440 AT1G10470.1 53.153 111 32 1 14 104 25 135 1.05e-30 109
Msa0371440 AT2G40670.1 47.170 106 33 1 22 104 28 133 3.40e-27 98.2
Msa0371440 AT3G56380.1 49.524 105 30 3 22 104 19 122 4.69e-27 97.4
Msa0371440 AT2G40670.2 46.729 107 33 2 22 104 28 134 1.32e-25 94.0
Msa0371440 AT3G56380.2 49.057 106 30 4 22 104 19 123 1.85e-25 93.6
Msa0371440 AT3G57040.2 43.590 117 36 1 18 104 4 120 3.85e-25 94.7
Msa0371440 AT3G57040.1 43.590 117 36 1 18 104 4 120 3.85e-25 94.7
Msa0371440 AT2G41310.1 43.750 112 35 2 21 104 7 118 9.12e-25 93.6

Find 107 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATTGCCACACAATTAT+AGG 0.180841 3_1:+69593556 Msa0371440:three_prime_UTR
AAAGATCTCAATAGCTAATT+AGG 0.190722 3_1:+69593100 Msa0371440:three_prime_UTR
AAGAAGATTACACTGTAAAT+AGG 0.246511 3_1:-69594319 None:intergenic
TGAACCTTCTCAGCCTTATA+AGG 0.246606 3_1:+69593648 Msa0371440:three_prime_UTR
TGTGAATTATGTATGGTATA+TGG 0.252952 3_1:+69592915 Msa0371440:three_prime_UTR
TTGCAAAATCTTTCTACTAA+TGG 0.257665 3_1:+69593362 Msa0371440:three_prime_UTR
ACATAGCCTTGGTGGTAAAT+TGG 0.285264 3_1:-69593234 None:intergenic
CATAATACTCAAATCATATT+AGG 0.289809 3_1:+69593059 Msa0371440:three_prime_UTR
TTAGAATGATGTTAAAGATT+TGG 0.295608 3_1:+69594292 Msa0371440:three_prime_UTR
TGGTGTTTGTCAATAATTTC+AGG 0.312578 3_1:-69593614 None:intergenic
GCAGGGTCCTTGTAGGAAAA+TGG 0.315431 3_1:-69593507 None:intergenic
CATTAAGCCTTCTTAATGAT+TGG 0.320570 3_1:+69593164 Msa0371440:three_prime_UTR
AGAAGATTACACTGTAAATA+GGG 0.323672 3_1:-69594318 None:intergenic
GAGAAAAGGGATTTATTGTT+TGG 0.330348 3_1:-69593289 None:intergenic
CTCATGCACATTCCCTTATA+AGG 0.332827 3_1:-69593661 None:intergenic
AAATGGATTTCATGTCTAGA+TGG 0.333475 3_1:-69592893 None:intergenic
AATCCTTTCACTAAAATGAT+AGG 0.335686 3_1:+69593328 Msa0371440:three_prime_UTR
CAAGATCTTACCTATCAATT+CGG 0.347926 3_1:-69592680 None:intergenic
TTTAGATTGGAATTGTAGTT+AGG 0.349100 3_1:-69594404 None:intergenic
CTCAATAGCTAATTAGGTTT+AGG 0.360086 3_1:+69593106 Msa0371440:three_prime_UTR
ACTAGAGCTTTACAGTATCT+TGG 0.363036 3_1:+69592219 Msa0371440:CDS
GACTAGTTATAGGTACATAT+TGG 0.364314 3_1:+69592791 Msa0371440:three_prime_UTR
AGCTTTACAGTATCTTGGTT+TGG 0.366392 3_1:+69592224 Msa0371440:CDS
AAGTAAGAAACTTCCATTCA+TGG 0.376704 3_1:-69593731 None:intergenic
GATGGTGACACGAGTTCTAT+TGG 0.385104 3_1:+69592246 Msa0371440:CDS
CTACTGCTGGTGATGTTCTC+AGG 0.387823 3_1:+69591937 Msa0371440:CDS
GAAGGTTCAACATAACAAAA+TGG 0.389001 3_1:-69593634 None:intergenic
AATGGTAAAGTGTTATATAA+AGG 0.400670 3_1:-69591802 None:intergenic
ATGATCAATAAATATGAAAC+AGG 0.413542 3_1:-69593759 None:intergenic
TGTTCTCAGGCAGAGTTTAC+CGG 0.414947 3_1:+69591950 Msa0371440:CDS
GTGAATTATGTATGGTATAT+GGG 0.417114 3_1:+69592916 Msa0371440:three_prime_UTR
ATGAAGAAGAAGTTTGTTGA+TGG 0.417706 3_1:-69591898 None:intergenic
GAACCTTCTCAGCCTTATAA+GGG 0.418934 3_1:+69593649 Msa0371440:three_prime_UTR
ATTAACTAAACCCTCCCATT+TGG 0.422471 3_1:+69594234 Msa0371440:three_prime_UTR
CATATTGCCAATCATTAAGA+AGG 0.425262 3_1:-69593171 None:intergenic
TTCACATCTGCCAACTTGAC+AGG 0.430476 3_1:-69593839 None:intergenic
ATGAATCAATGGTTATAGTC+TGG 0.434730 3_1:-69594023 None:intergenic
CTGACAATGAGTATAGGATT+AGG 0.443189 3_1:+69594129 Msa0371440:three_prime_UTR
ACATCTTGACCCGAATTGAT+AGG 0.448548 3_1:+69592670 Msa0371440:CDS
ACACCAGCACCACTCACTTC+CGG 0.448562 3_1:-69591969 None:intergenic
ATAACCATTGATTCATTTAG+CGG 0.455397 3_1:+69594030 Msa0371440:three_prime_UTR
AATAATGTTGTCATGCCAGC+AGG 0.457290 3_1:-69593525 None:intergenic
GGCCATGTGTTGGTGAGAAA+AGG 0.459772 3_1:-69593303 None:intergenic
TACCATACATAATTCACAAA+TGG 0.460497 3_1:-69592910 None:intergenic
CATGCCAGCAGGGTCCTTGT+AGG 0.461652 3_1:-69593514 None:intergenic
CTTAAATTCACTTCTTGCAA+AGG 0.463478 3_1:+69592056 Msa0371440:CDS
CAAATTTGATGATCCATGAA+TGG 0.469303 3_1:+69593718 Msa0371440:three_prime_UTR
TTGAAGTAGACCCTGCCAAA+TGG 0.471084 3_1:-69594249 None:intergenic
ACTAAACCCTCCCATTTGGC+AGG 0.473859 3_1:+69594238 Msa0371440:three_prime_UTR
ATTGACAGTTGCTTGGAAGA+AGG 0.475916 3_1:+69593794 Msa0371440:three_prime_UTR
ATCCATTTGTGAATTATGTA+TGG 0.475964 3_1:+69592908 Msa0371440:three_prime_UTR
TAACATGGTGATATTATTGA+GGG 0.476877 3_1:-69593396 None:intergenic
TTACAGTATCTTGGTTTGGA+TGG 0.481557 3_1:+69592228 Msa0371440:CDS
TGAAGTAGACCCTGCCAAAT+GGG 0.496813 3_1:-69594248 None:intergenic
CTAACATGGTGATATTATTG+AGG 0.496837 3_1:-69593397 None:intergenic
AGGGATCAAGCTTAAAATCT+CGG 0.498907 3_1:-69592762 None:intergenic
AAGATCTTACCTATCAATTC+GGG 0.507592 3_1:-69592679 None:intergenic
TTTGTTCATTGACAGTTGCT+TGG 0.513588 3_1:+69593787 Msa0371440:three_prime_UTR
AATGGATTTCATGTCTAGAT+GGG 0.515419 3_1:-69592892 None:intergenic
GTTTAGGCTGGCACTGAATG+TGG 0.516455 3_1:+69593122 Msa0371440:three_prime_UTR
AGAGGTGAAGGAAAGAAAGT+CGG 0.518178 3_1:+69593884 Msa0371440:three_prime_UTR
AAACAATACTAATTCAAAAG+AGG 0.519546 3_1:-69592716 None:intergenic
AAGAGAAAGAGAGAGAGATA+CGG 0.521048 3_1:-69591776 None:intergenic
ATAGCTAATTAGGTTTAGGC+TGG 0.523150 3_1:+69593110 Msa0371440:three_prime_UTR
GGCACCGCTAAATGAATCAA+TGG 0.523340 3_1:-69594034 None:intergenic
TTACCGGAAGTGAGTGGTGC+TGG 0.527573 3_1:+69591966 Msa0371440:CDS
AAATTGCTGACAATGAGTAT+AGG 0.533650 3_1:+69594123 Msa0371440:three_prime_UTR
ATAATTTCAGGAATAAAAGT+AGG 0.534020 3_1:-69593602 None:intergenic
GCCATGTGTTGGTGAGAAAA+GGG 0.535915 3_1:-69593302 None:intergenic
AAAATACAACATAGCCTTGG+TGG 0.538703 3_1:-69593242 None:intergenic
TTGATAGGTAAGATCTTGAT+CGG 0.538912 3_1:+69592685 Msa0371440:exon
TTGATCGTAAAGTCATTGAA+AGG 0.541223 3_1:+69592030 Msa0371440:CDS
GAAGTGAGTGGTGCTGGTGT+TGG 0.542048 3_1:+69591972 Msa0371440:CDS
TTGTATTTCATCTCTATCAA+CGG 0.543618 3_1:+69593943 Msa0371440:three_prime_UTR
CATGGATCATCAAATTTGCA+AGG 0.548667 3_1:-69593713 None:intergenic
TCAGAGGACATAACCACCAC+TGG 0.556667 3_1:-69592645 None:intergenic
TCTATTGAAACCTGTCAAGT+TGG 0.561378 3_1:+69593829 Msa0371440:three_prime_UTR
ATTCCCTTATAAGGCTGAGA+AGG 0.565598 3_1:-69593652 None:intergenic
TTCTTTGACGACATTGCAGA+CGG 0.571495 3_1:-69593998 None:intergenic
GGTGAAGGAAAGAAAGTCGG+AGG 0.571633 3_1:+69593887 Msa0371440:three_prime_UTR
CCATGTGTTTAACTCTAACA+TGG 0.572453 3_1:-69593411 None:intergenic
ACCTATAACTAGTCAAAACA+AGG 0.579041 3_1:-69592782 None:intergenic
AGAGTTGCACGTTCTTGCAG+TGG 0.580799 3_1:+69591995 Msa0371440:CDS
GAAGATTACACTGTAAATAG+GGG 0.585833 3_1:-69594317 None:intergenic
TAGGATTAGGACTGATGAAG+AGG 0.592905 3_1:+69594142 Msa0371440:three_prime_UTR
ATTTAGAGAAATTCCAGTGG+TGG 0.593101 3_1:+69592632 Msa0371440:CDS
GATTTCATAATGAGAGGTGA+AGG 0.593144 3_1:+69593872 Msa0371440:three_prime_UTR
TTAACAGATTTCATAATGAG+AGG 0.593540 3_1:+69593866 Msa0371440:three_prime_UTR
GACATTGCAGACGGTGACAA+TGG 0.595350 3_1:-69593989 None:intergenic
TTGCTTGGAAGAAGGAGCAG+AGG 0.596704 3_1:+69593802 Msa0371440:three_prime_UTR
CTAAACCCTCCCATTTGGCA+GGG 0.601382 3_1:+69594239 Msa0371440:three_prime_UTR
AATATGGTTACAGGGTGTGA+AGG 0.609461 3_1:+69592370 Msa0371440:intron
GTAGACCCTGCCAAATGGGA+GGG 0.631769 3_1:-69594244 None:intergenic
AGTAGACCCTGCCAAATGGG+AGG 0.633441 3_1:-69594245 None:intergenic
GCAGTTACTGTTGTAGAGAG+TGG 0.636724 3_1:+69592195 Msa0371440:intron
AGAGTTAAACACATGGAGTG+TGG 0.641750 3_1:+69593418 Msa0371440:three_prime_UTR
AGAAAGAGAGAGAGATACGG+AGG 0.646002 3_1:-69591773 None:intergenic
TGATAAAACCTATAATTGTG+TGG 0.657257 3_1:-69593564 None:intergenic
ATGATTATGATCACCCACCA+TGG 0.659262 3_1:-69593039 None:intergenic
CAGAGTTTACCGGAAGTGAG+TGG 0.668612 3_1:+69591960 Msa0371440:CDS
AAGATTACACTGTAAATAGG+GGG 0.670911 3_1:-69594316 None:intergenic
CCATGTTAGAGTTAAACACA+TGG 0.671166 3_1:+69593411 Msa0371440:three_prime_UTR
ATAATGTTGTCATGCCAGCA+GGG 0.671199 3_1:-69593524 None:intergenic
ATATTACCAATTTACCACCA+AGG 0.677760 3_1:+69593228 Msa0371440:three_prime_UTR
CCTATAACTAGTCAAAACAA+GGG 0.693281 3_1:-69592781 None:intergenic
TGTATTTAGAGAAATTCCAG+TGG 0.707997 3_1:+69592629 Msa0371440:CDS
TCGGGTCAAGATGTTCTCAG+AGG 0.723155 3_1:-69592661 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAATAATTGCAT+AGG - chr3_1:69592835-69592854 None:intergenic 10.0%
!!! TTAAATACATGTATATTTTT+GGG + chr3_1:69592862-69592881 Msa0371440:three_prime_UTR 10.0%
!! AATACATCAAAAAAACAAAA+GGG - chr3_1:69592106-69592125 None:intergenic 15.0%
!! AAATACATCAAAAAAACAAA+AGG - chr3_1:69592107-69592126 None:intergenic 15.0%
!!! GAATTTTTTTTTTTTTGTTG+AGG + chr3_1:69592303-69592322 Msa0371440:intron 15.0%
!!! TTTGAATTAGTATTGTTTTA+AGG + chr3_1:69592721-69592740 Msa0371440:three_prime_UTR 15.0%
!!! GTTAAATACATGTATATTTT+TGG + chr3_1:69592861-69592880 Msa0371440:three_prime_UTR 15.0%
!!! TTTTTTTAAGCAAAAAATCG+AGG - chr3_1:69591728-69591747 None:intergenic 20.0%
!!! AATGGTAAAGTGTTATATAA+AGG - chr3_1:69591805-69591824 None:intergenic 20.0%
!!! TTTTTGAAGAAGAAGAAAAA+TGG - chr3_1:69591823-69591842 None:intergenic 20.0%
!!! AACTTGTTTTTTTTTTTGTG+TGG + chr3_1:69592173-69592192 Msa0371440:intron 20.0%
!!! GTATTTTTAGTTTTTGTGTT+TGG + chr3_1:69592325-69592344 Msa0371440:intron 20.0%
!! AAACAATACTAATTCAAAAG+AGG - chr3_1:69592719-69592738 None:intergenic 20.0%
!! CATAATACTCAAATCATATT+AGG + chr3_1:69593059-69593078 Msa0371440:three_prime_UTR 20.0%
!! ATAATTTCAGGAATAAAAGT+AGG - chr3_1:69593605-69593624 None:intergenic 20.0%
!! ATGATCAATAAATATGAAAC+AGG - chr3_1:69593762-69593781 None:intergenic 20.0%
!! AAATATCTCAAAAAAGAAGT+CGG + chr3_1:69593912-69593931 Msa0371440:three_prime_UTR 20.0%
!! TTAGAATGATGTTAAAGATT+TGG + chr3_1:69594292-69594311 Msa0371440:three_prime_UTR 20.0%
!!! TCTTATTTTACTGTCTTTTT+TGG + chr3_1:69594338-69594357 Msa0371440:three_prime_UTR 20.0%
! TCTTGTATTCTTGATTCATT+TGG + chr3_1:69592128-69592147 Msa0371440:intron 25.0%
! TATTCTTGATTCATTTGGAT+TGG + chr3_1:69592133-69592152 Msa0371440:intron 25.0%
!!! TCATTTGGATTGGATTTTAA+TGG + chr3_1:69592143-69592162 Msa0371440:intron 25.0%
!!! GATTGGATTTTAATGGTTTT+TGG + chr3_1:69592150-69592169 Msa0371440:intron 25.0%
!! GTAAGCAAAGTTTCTAATTT+TGG + chr3_1:69592276-69592295 Msa0371440:intron 25.0%
!!! TGTTGTTGTTTTGATGAATA+TGG + chr3_1:69592354-69592373 Msa0371440:intron 25.0%
!! TTTTGATGAATATGGTTACA+GGG + chr3_1:69592362-69592381 Msa0371440:intron 25.0%
!!! TTTGTTTTCTTAGAGATTAG+AGG + chr3_1:69592484-69592503 Msa0371440:intron 25.0%
!!! TTATTTCTGTTAATTTCAGC+AGG + chr3_1:69592599-69592618 Msa0371440:intron 25.0%
! ATCCATTTGTGAATTATGTA+TGG + chr3_1:69592908-69592927 Msa0371440:three_prime_UTR 25.0%
! TACCATACATAATTCACAAA+TGG - chr3_1:69592913-69592932 None:intergenic 25.0%
! TGTGAATTATGTATGGTATA+TGG + chr3_1:69592915-69592934 Msa0371440:three_prime_UTR 25.0%
! GTGAATTATGTATGGTATAT+GGG + chr3_1:69592916-69592935 Msa0371440:three_prime_UTR 25.0%
! AAAGATCTCAATAGCTAATT+AGG + chr3_1:69593100-69593119 Msa0371440:three_prime_UTR 25.0%
!!! TCATTTTAGTGAAAGGATTT+TGG - chr3_1:69593327-69593346 None:intergenic 25.0%
! AATCCTTTCACTAAAATGAT+AGG + chr3_1:69593328-69593347 Msa0371440:three_prime_UTR 25.0%
!!! TAACCTATCATTTTAGTGAA+AGG - chr3_1:69593334-69593353 None:intergenic 25.0%
! TTGCAAAATCTTTCTACTAA+TGG + chr3_1:69593362-69593381 Msa0371440:three_prime_UTR 25.0%
! TAACATGGTGATATTATTGA+GGG - chr3_1:69593399-69593418 None:intergenic 25.0%
! TATAATTGCCACACAATTAT+AGG + chr3_1:69593556-69593575 Msa0371440:three_prime_UTR 25.0%
! TGATAAAACCTATAATTGTG+TGG - chr3_1:69593567-69593586 None:intergenic 25.0%
! TTAACAGATTTCATAATGAG+AGG + chr3_1:69593866-69593885 Msa0371440:three_prime_UTR 25.0%
! TTGTATTTCATCTCTATCAA+CGG + chr3_1:69593943-69593962 Msa0371440:three_prime_UTR 25.0%
!!! ATAACCATTGATTCATTTAG+CGG + chr3_1:69594030-69594049 Msa0371440:three_prime_UTR 25.0%
! AGAAGATTACACTGTAAATA+GGG - chr3_1:69594321-69594340 None:intergenic 25.0%
! AAGAAGATTACACTGTAAAT+AGG - chr3_1:69594322-69594341 None:intergenic 25.0%
! TTTAGATTGGAATTGTAGTT+AGG - chr3_1:69594407-69594426 None:intergenic 25.0%
!! ATGAAGAAGAAGTTTGTTGA+TGG - chr3_1:69591901-69591920 None:intergenic 30.0%
TTGATCGTAAAGTCATTGAA+AGG + chr3_1:69592030-69592049 Msa0371440:CDS 30.0%
CTTAAATTCACTTCTTGCAA+AGG + chr3_1:69592056-69592075 Msa0371440:CDS 30.0%
!! GTTTTGATGAATATGGTTAC+AGG + chr3_1:69592361-69592380 Msa0371440:intron 30.0%
TGAATTGCTCAAGAAGATTA+AGG + chr3_1:69592439-69592458 Msa0371440:intron 30.0%
GTCTACAATCTAATCTAATC+AGG - chr3_1:69592536-69592555 None:intergenic 30.0%
TGTATTTAGAGAAATTCCAG+TGG + chr3_1:69592629-69592648 Msa0371440:CDS 30.0%
AAGATCTTACCTATCAATTC+GGG - chr3_1:69592682-69592701 None:intergenic 30.0%
CAAGATCTTACCTATCAATT+CGG - chr3_1:69592683-69592702 None:intergenic 30.0%
TTGATAGGTAAGATCTTGAT+CGG + chr3_1:69592685-69592704 Msa0371440:exon 30.0%
AATCTCGGCTAAAAAACAAT+AGG - chr3_1:69592750-69592769 None:intergenic 30.0%
CCTATAACTAGTCAAAACAA+GGG - chr3_1:69592784-69592803 None:intergenic 30.0%
ACCTATAACTAGTCAAAACA+AGG - chr3_1:69592785-69592804 None:intergenic 30.0%
! GACTAGTTATAGGTACATAT+TGG + chr3_1:69592791-69592810 Msa0371440:three_prime_UTR 30.0%
! AATGGATTTCATGTCTAGAT+GGG - chr3_1:69592895-69592914 None:intergenic 30.0%
! AAATGGATTTCATGTCTAGA+TGG - chr3_1:69592896-69592915 None:intergenic 30.0%
TAGAATAAGCAAAAACGTGT+GGG - chr3_1:69592951-69592970 None:intergenic 30.0%
CTCAATAGCTAATTAGGTTT+AGG + chr3_1:69593106-69593125 Msa0371440:three_prime_UTR 30.0%
CATTAAGCCTTCTTAATGAT+TGG + chr3_1:69593164-69593183 Msa0371440:three_prime_UTR 30.0%
CATATTGCCAATCATTAAGA+AGG - chr3_1:69593174-69593193 None:intergenic 30.0%
ATATTACCAATTTACCACCA+AGG + chr3_1:69593228-69593247 Msa0371440:three_prime_UTR 30.0%
ACAAAAATACAACATAGCCT+TGG - chr3_1:69593248-69593267 None:intergenic 30.0%
GAGAAAAGGGATTTATTGTT+TGG - chr3_1:69593292-69593311 None:intergenic 30.0%
CTAACATGGTGATATTATTG+AGG - chr3_1:69593400-69593419 None:intergenic 30.0%
! ATGAAATCCATTTTCCTACA+AGG + chr3_1:69593500-69593519 Msa0371440:three_prime_UTR 30.0%
TGGTGTTTGTCAATAATTTC+AGG - chr3_1:69593617-69593636 None:intergenic 30.0%
GAAGGTTCAACATAACAAAA+TGG - chr3_1:69593637-69593656 None:intergenic 30.0%
! CAAATTTGATGATCCATGAA+TGG + chr3_1:69593718-69593737 Msa0371440:three_prime_UTR 30.0%
AAGTAAGAAACTTCCATTCA+TGG - chr3_1:69593734-69593753 None:intergenic 30.0%
ATGAATCAATGGTTATAGTC+TGG - chr3_1:69594026-69594045 None:intergenic 30.0%
AGCAAAAATGGAGACATAAA+GGG - chr3_1:69594058-69594077 None:intergenic 30.0%
GAACATTAGTTCAGCAAAAA+TGG - chr3_1:69594070-69594089 None:intergenic 30.0%
AAATTGCTGACAATGAGTAT+AGG + chr3_1:69594123-69594142 Msa0371440:three_prime_UTR 30.0%
AAGATTACACTGTAAATAGG+GGG - chr3_1:69594319-69594338 None:intergenic 30.0%
GAAGATTACACTGTAAATAG+GGG - chr3_1:69594320-69594339 None:intergenic 30.0%
AAGAGAAAGAGAGAGAGATA+CGG - chr3_1:69591779-69591798 None:intergenic 35.0%
ACTAGAGCTTTACAGTATCT+TGG + chr3_1:69592219-69592238 Msa0371440:CDS 35.0%
AGCTTTACAGTATCTTGGTT+TGG + chr3_1:69592224-69592243 Msa0371440:CDS 35.0%
!! TTACAGTATCTTGGTTTGGA+TGG + chr3_1:69592228-69592247 Msa0371440:CDS 35.0%
! TAGAGGTGTATGTATTGAAG+AGG + chr3_1:69592501-69592520 Msa0371440:intron 35.0%
ATTTAGAGAAATTCCAGTGG+TGG + chr3_1:69592632-69592651 Msa0371440:CDS 35.0%
AGGGATCAAGCTTAAAATCT+CGG - chr3_1:69592765-69592784 None:intergenic 35.0%
!!! CCCTTGTTTTGACTAGTTAT+AGG + chr3_1:69592781-69592800 Msa0371440:three_prime_UTR 35.0%
CTAGAATAAGCAAAAACGTG+TGG - chr3_1:69592952-69592971 None:intergenic 35.0%
! CACACGTTTTTGCTTATTCT+AGG + chr3_1:69592950-69592969 Msa0371440:three_prime_UTR 35.0%
! CGTTTTTGCTTATTCTAGGT+TGG + chr3_1:69592954-69592973 Msa0371440:three_prime_UTR 35.0%
ATAGCTAATTAGGTTTAGGC+TGG + chr3_1:69593110-69593129 Msa0371440:three_prime_UTR 35.0%
! AAAATACAACATAGCCTTGG+TGG - chr3_1:69593245-69593264 None:intergenic 35.0%
CCATGTGTTTAACTCTAACA+TGG - chr3_1:69593414-69593433 None:intergenic 35.0%
CCATGTTAGAGTTAAACACA+TGG + chr3_1:69593411-69593430 Msa0371440:three_prime_UTR 35.0%
CATGGATCATCAAATTTGCA+AGG - chr3_1:69593716-69593735 None:intergenic 35.0%
TTTGTTCATTGACAGTTGCT+TGG + chr3_1:69593787-69593806 Msa0371440:three_prime_UTR 35.0%
TCTATTGAAACCTGTCAAGT+TGG + chr3_1:69593829-69593848 Msa0371440:three_prime_UTR 35.0%
GATTTCATAATGAGAGGTGA+AGG + chr3_1:69593872-69593891 Msa0371440:three_prime_UTR 35.0%
CAGCAAAAATGGAGACATAA+AGG - chr3_1:69594059-69594078 None:intergenic 35.0%
CTGACAATGAGTATAGGATT+AGG + chr3_1:69594129-69594148 Msa0371440:three_prime_UTR 35.0%
ATTAACTAAACCCTCCCATT+TGG + chr3_1:69594234-69594253 Msa0371440:three_prime_UTR 35.0%
!! TGGTAATGAGTTTTGCTACA+AGG + chr3_1:69594358-69594377 Msa0371440:three_prime_UTR 35.0%
AAGCAAAAAATCGAGGACAG+AGG - chr3_1:69591721-69591740 None:intergenic 40.0%
!! TCTTCATCGCTTTTCTGTGA+TGG + chr3_1:69591914-69591933 Msa0371440:exon 40.0%
AATATGGTTACAGGGTGTGA+AGG + chr3_1:69592370-69592389 Msa0371440:intron 40.0%
ATGACTGATTATTCCATGCC+TGG + chr3_1:69592404-69592423 Msa0371440:intron 40.0%
TGACTGATTATTCCATGCCT+GGG + chr3_1:69592405-69592424 Msa0371440:intron 40.0%
AATTCATATCCTGTCATCCC+AGG - chr3_1:69592425-69592444 None:intergenic 40.0%
ACATCTTGACCCGAATTGAT+AGG + chr3_1:69592670-69592689 Msa0371440:CDS 40.0%
!!! ATGGCTGTTTTTGGATTTGG+TGG - chr3_1:69593023-69593042 None:intergenic 40.0%
ACCAAATCCAAAAACAGCCA+TGG + chr3_1:69593022-69593041 Msa0371440:three_prime_UTR 40.0%
!!! ACCATGGCTGTTTTTGGATT+TGG - chr3_1:69593026-69593045 None:intergenic 40.0%
AAATCCAAAAACAGCCATGG+TGG + chr3_1:69593025-69593044 Msa0371440:three_prime_UTR 40.0%
AATCCAAAAACAGCCATGGT+GGG + chr3_1:69593026-69593045 Msa0371440:three_prime_UTR 40.0%
ATGATTATGATCACCCACCA+TGG - chr3_1:69593042-69593061 None:intergenic 40.0%
! ACATAGCCTTGGTGGTAAAT+TGG - chr3_1:69593237-69593256 None:intergenic 40.0%
!! AAAGGATTTTGGCCATGTGT+TGG - chr3_1:69593316-69593335 None:intergenic 40.0%
AGAGTTAAACACATGGAGTG+TGG + chr3_1:69593418-69593437 Msa0371440:three_prime_UTR 40.0%
ATAATGTTGTCATGCCAGCA+GGG - chr3_1:69593527-69593546 None:intergenic 40.0%
AATAATGTTGTCATGCCAGC+AGG - chr3_1:69593528-69593547 None:intergenic 40.0%
! TGAACCTTCTCAGCCTTATA+AGG + chr3_1:69593648-69593667 Msa0371440:three_prime_UTR 40.0%
! GAACCTTCTCAGCCTTATAA+GGG + chr3_1:69593649-69593668 Msa0371440:three_prime_UTR 40.0%
ATTCCCTTATAAGGCTGAGA+AGG - chr3_1:69593655-69593674 None:intergenic 40.0%
CTCATGCACATTCCCTTATA+AGG - chr3_1:69593664-69593683 None:intergenic 40.0%
ATTGACAGTTGCTTGGAAGA+AGG + chr3_1:69593794-69593813 Msa0371440:three_prime_UTR 40.0%
AGAGGTGAAGGAAAGAAAGT+CGG + chr3_1:69593884-69593903 Msa0371440:three_prime_UTR 40.0%
TTCTTTGACGACATTGCAGA+CGG - chr3_1:69594001-69594020 None:intergenic 40.0%
TAGGATTAGGACTGATGAAG+AGG + chr3_1:69594142-69594161 Msa0371440:three_prime_UTR 40.0%
AGAAAGAGAGAGAGATACGG+AGG - chr3_1:69591776-69591795 None:intergenic 45.0%
! TTTTCTGTGATGGCTACTGC+TGG + chr3_1:69591924-69591943 Msa0371440:exon 45.0%
TGTTCTCAGGCAGAGTTTAC+CGG + chr3_1:69591950-69591969 Msa0371440:CDS 45.0%
GCAGTTACTGTTGTAGAGAG+TGG + chr3_1:69592195-69592214 Msa0371440:intron 45.0%
GATGGTGACACGAGTTCTAT+TGG + chr3_1:69592246-69592265 Msa0371440:CDS 45.0%
! TCCCTTTTCTCACCAACACA+TGG + chr3_1:69593301-69593320 Msa0371440:three_prime_UTR 45.0%
! GCCATGTGTTGGTGAGAAAA+GGG - chr3_1:69593305-69593324 None:intergenic 45.0%
TTCACATCTGCCAACTTGAC+AGG - chr3_1:69593842-69593861 None:intergenic 45.0%
GGCACCGCTAAATGAATCAA+TGG - chr3_1:69594037-69594056 None:intergenic 45.0%
TGAAGTAGACCCTGCCAAAT+GGG - chr3_1:69594251-69594270 None:intergenic 45.0%
TTGAAGTAGACCCTGCCAAA+TGG - chr3_1:69594252-69594271 None:intergenic 45.0%
! CTACTGCTGGTGATGTTCTC+AGG + chr3_1:69591937-69591956 Msa0371440:CDS 50.0%
CAGAGTTTACCGGAAGTGAG+TGG + chr3_1:69591960-69591979 Msa0371440:CDS 50.0%
AGAGTTGCACGTTCTTGCAG+TGG + chr3_1:69591995-69592014 Msa0371440:CDS 50.0%
TATTCCATGCCTGGGATGAC+AGG + chr3_1:69592413-69592432 Msa0371440:intron 50.0%
ATATCCTGTCATCCCAGGCA+TGG - chr3_1:69592420-69592439 None:intergenic 50.0%
TCAGAGGACATAACCACCAC+TGG - chr3_1:69592648-69592667 None:intergenic 50.0%
TCGGGTCAAGATGTTCTCAG+AGG - chr3_1:69592664-69592683 None:intergenic 50.0%
!! TCACCCACCATGGCTGTTTT+TGG - chr3_1:69593032-69593051 None:intergenic 50.0%
! GTTTAGGCTGGCACTGAATG+TGG + chr3_1:69593122-69593141 Msa0371440:three_prime_UTR 50.0%
! GGCCATGTGTTGGTGAGAAA+AGG - chr3_1:69593306-69593325 None:intergenic 50.0%
GCAGGGTCCTTGTAGGAAAA+TGG - chr3_1:69593510-69593529 None:intergenic 50.0%
! TTTTCCTACAAGGACCCTGC+TGG + chr3_1:69593510-69593529 Msa0371440:three_prime_UTR 50.0%
TTGCTTGGAAGAAGGAGCAG+AGG + chr3_1:69593802-69593821 Msa0371440:three_prime_UTR 50.0%
GGTGAAGGAAAGAAAGTCGG+AGG + chr3_1:69593887-69593906 Msa0371440:three_prime_UTR 50.0%
GACATTGCAGACGGTGACAA+TGG - chr3_1:69593992-69594011 None:intergenic 50.0%
ACTAAACCCTCCCATTTGGC+AGG + chr3_1:69594238-69594257 Msa0371440:three_prime_UTR 50.0%
CTAAACCCTCCCATTTGGCA+GGG + chr3_1:69594239-69594258 Msa0371440:three_prime_UTR 50.0%
TTACCGGAAGTGAGTGGTGC+TGG + chr3_1:69591966-69591985 Msa0371440:CDS 55.0%
ACACCAGCACCACTCACTTC+CGG - chr3_1:69591972-69591991 None:intergenic 55.0%
!! GAAGTGAGTGGTGCTGGTGT+TGG + chr3_1:69591972-69591991 Msa0371440:CDS 55.0%
GTAGACCCTGCCAAATGGGA+GGG - chr3_1:69594247-69594266 None:intergenic 55.0%
AGTAGACCCTGCCAAATGGG+AGG - chr3_1:69594248-69594267 None:intergenic 55.0%
CATGCCAGCAGGGTCCTTGT+AGG - chr3_1:69593517-69593536 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 69591712 69594438 69591712 ID=Msa0371440;Name=Msa0371440
chr3_1 mRNA 69591712 69594438 69591712 ID=Msa0371440-mRNA-1;Parent=Msa0371440;Name=Msa0371440-mRNA-1;_AED=0.03;_eAED=0.03;_QI=221|0.5|0.6|0.8|1|1|5|1743|104
chr3_1 exon 69591712 69592077 69591712 ID=Msa0371440-mRNA-1:exon:14878;Parent=Msa0371440-mRNA-1
chr3_1 exon 69592199 69592275 69592199 ID=Msa0371440-mRNA-1:exon:14879;Parent=Msa0371440-mRNA-1
chr3_1 exon 69592383 69592391 69592383 ID=Msa0371440-mRNA-1:exon:14880;Parent=Msa0371440-mRNA-1
chr3_1 exon 69592497 69592505 69592497 ID=Msa0371440-mRNA-1:exon:14881;Parent=Msa0371440-mRNA-1
chr3_1 exon 69592621 69594438 69592621 ID=Msa0371440-mRNA-1:exon:14882;Parent=Msa0371440-mRNA-1
chr3_1 five_prime_UTR 69591712 69591932 69591712 ID=Msa0371440-mRNA-1:five_prime_utr;Parent=Msa0371440-mRNA-1
chr3_1 CDS 69591933 69592077 69591933 ID=Msa0371440-mRNA-1:cds;Parent=Msa0371440-mRNA-1
chr3_1 CDS 69592199 69592275 69592199 ID=Msa0371440-mRNA-1:cds;Parent=Msa0371440-mRNA-1
chr3_1 CDS 69592383 69592391 69592383 ID=Msa0371440-mRNA-1:cds;Parent=Msa0371440-mRNA-1
chr3_1 CDS 69592497 69592505 69592497 ID=Msa0371440-mRNA-1:cds;Parent=Msa0371440-mRNA-1
chr3_1 CDS 69592621 69592695 69592621 ID=Msa0371440-mRNA-1:cds;Parent=Msa0371440-mRNA-1
chr3_1 three_prime_UTR 69592696 69594438 69592696 ID=Msa0371440-mRNA-1:three_prime_utr;Parent=Msa0371440-mRNA-1
Gene Sequence

>Msa0371440

ATGGCTACTGCTGGTGATGTTCTCAGGCAGAGTTTACCGGAAGTGAGTGGTGCTGGTGTTGGAGAGTTGCACGTTCTTGCAGTGGATGATAGTTTTGTTGATCGTAAAGTCATTGAAAGGTTGCTTAAATTCACTTCTTGCAAAGTTACTGTTGTAGAGAGTGGAACTAGAGCTTTACAGTATCTTGGTTTGGATGGTGACACGAGTTCTATTGGTTTTGATGGTGTGAAGAGATTAGAGGAATCATCTGTATTTAGAGAAATTCCAGTGGTGGTTATGTCCTCTGAGAACATCTTGACCCGAATTGATAGGTAA
Protein sequence

>Msa0371440

MATAGDVLRQSLPEVSGAGVGELHVLAVDDSFVDRKVIERLLKFTSCKVTVVESGTRALQYLGLDGDTSSIGFDGVKRLEESSVFREIPVVVMSSENILTRIDR*