AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0450200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0450200 MtrunA17_Chr3g0118211 81.301 123 3 1 1 103 1 123 2.90e-62 187
Msa0450200 MtrunA17_Chr4g0059571 59.055 127 29 2 1 104 1 127 1.46e-42 138
Msa0450200 MtrunA17_Chr1g0170741 51.887 106 28 1 22 104 7 112 1.28e-28 102
Msa0450200 MtrunA17_Chr5g0414931 51.786 112 30 2 17 104 10 121 7.32e-27 99.0
Msa0450200 MtrunA17_Chr7g0256551 43.103 116 37 2 18 104 4 119 9.61e-23 88.2
Msa0450200 MtrunA17_Chr3g0128691 40.777 103 39 2 24 104 19 121 7.67e-20 79.0
Msa0450200 MtrunA17_Chr3g0082861 42.105 114 37 2 20 104 1 114 8.56e-20 79.0
Msa0450200 MtrunA17_Chr8g0352871 40.870 115 40 2 18 104 4 118 1.20e-19 79.0
Msa0450200 MtrunA17_Chr3g0124861 38.983 118 33 2 23 102 22 138 3.71e-17 73.6
Msa0450200 MtrunA17_Chr4g0021731 51.786 56 27 0 15 70 3 58 9.81e-13 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0450200 AT3G48100.1 54.762 126 31 3 5 104 2 127 2.77e-35 119
Msa0450200 AT1G74890.1 60.194 103 21 1 22 104 17 119 6.89e-34 116
Msa0450200 AT5G62920.1 58.824 102 22 1 23 104 25 126 3.21e-33 114
Msa0450200 AT1G19050.1 55.000 120 32 2 5 102 4 123 4.35e-33 114
Msa0450200 AT1G59940.1 49.265 136 35 4 1 104 1 134 4.61e-31 109
Msa0450200 AT1G59940.2 49.265 136 35 4 1 104 93 226 2.39e-30 110
Msa0450200 AT1G10470.3 53.153 111 32 1 14 104 11 121 3.39e-30 108
Msa0450200 AT1G10470.1 53.153 111 32 1 14 104 25 135 7.11e-30 107
Msa0450200 AT2G40670.2 46.729 107 33 2 22 104 28 134 1.10e-25 94.4
Msa0450200 AT2G40670.1 47.170 106 33 1 22 104 28 133 1.16e-25 94.4
Msa0450200 AT3G56380.1 49.524 105 30 3 22 104 19 122 1.45e-25 93.6
Msa0450200 AT3G56380.2 49.057 106 30 4 22 104 19 123 1.75e-25 93.6
Msa0450200 AT3G57040.2 43.590 117 36 1 18 104 4 120 1.36e-23 90.5
Msa0450200 AT3G57040.1 43.590 117 36 1 18 104 4 120 1.36e-23 90.5
Msa0450200 AT2G41310.1 43.750 112 35 2 21 104 7 118 3.46e-23 89.4

Find 103 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATTGCCACACAATTAT+AGG 0.180841 3_3:-35547457 Msa0450200:three_prime_UTR
AAAGATCTCAATATCTAATT+AGG 0.216835 3_3:-35547913 Msa0450200:three_prime_UTR
AAGAAGATTACACTGTAAAT+AGG 0.246511 3_3:+35546694 None:intergenic
TGAACCTTCTCAGCCTTATA+AGG 0.246606 3_3:-35547365 Msa0450200:three_prime_UTR
TTGCAAAATCTTTCTACTAA+TGG 0.257665 3_3:-35547651 Msa0450200:three_prime_UTR
ACATAGCCTTGGTGGTAAAT+TGG 0.285264 3_3:+35547779 None:intergenic
TTAGAATGATGTTAAAGATT+TGG 0.295608 3_3:-35546721 Msa0450200:three_prime_UTR
TGGTGTTTGTCAATAATTTC+AGG 0.312578 3_3:+35547399 None:intergenic
GCAGGGTCCTTGTAGGAAAA+TGG 0.315431 3_3:+35547506 None:intergenic
CATTAAGCCTTCTTAATGAT+TGG 0.320570 3_3:-35547849 Msa0450200:three_prime_UTR
TGTGAAATATGTATGGAATA+TGG 0.321658 3_3:-35548097 Msa0450200:three_prime_UTR
AGAAGATTACACTGTAAATA+GGG 0.323672 3_3:+35546695 None:intergenic
GAGAAAAGGGATTTATTGTT+TGG 0.330348 3_3:+35547724 None:intergenic
CTCATGCACATTCCCTTATA+AGG 0.332827 3_3:+35547352 None:intergenic
AAATGGATTTCATGTCTAGA+TGG 0.333475 3_3:+35548119 None:intergenic
AATCCTTTCACTAAAATGAT+AGG 0.335686 3_3:-35547685 Msa0450200:three_prime_UTR
CTCAATATCTAATTAGGTTT+AGG 0.337955 3_3:-35547907 Msa0450200:three_prime_UTR
CAAGATCTTACCTATCAATT+CGG 0.347926 3_3:+35548344 None:intergenic
TTTAGATTGGAATTGTAGTT+AGG 0.349100 3_3:+35546609 None:intergenic
ACTAGAGCTTTACAGTATCT+TGG 0.363036 3_3:-35548825 Msa0450200:CDS
AGCTTTACAGTATCTTGGTT+TGG 0.366392 3_3:-35548820 Msa0450200:CDS
GATTAGTTATAGTTACATAT+TGG 0.366901 3_3:-35548233 Msa0450200:three_prime_UTR
AAGTAAGAAACTTCCATTCA+TGG 0.376704 3_3:+35547282 None:intergenic
CTACTGCTGGTGATGTTCTC+AGG 0.387823 3_3:-35549095 Msa0450200:CDS
GAAGGTTCAACATAACAAAA+TGG 0.389001 3_3:+35547379 None:intergenic
TTCCATACATATTTCACAAA+TGG 0.394755 3_3:+35548102 None:intergenic
AATGGCAAAGTGTTATATAA+AGG 0.400670 3_3:+35549230 None:intergenic
ATGATCAATAAATATGAAAC+AGG 0.413542 3_3:+35547254 None:intergenic
TGTTCTCAGGCAGAGTTTAC+CGG 0.414947 3_3:-35549082 Msa0450200:CDS
ATGAAGAAGAAGTTTGTTGA+TGG 0.417706 3_3:+35549134 None:intergenic
GAACCTTCTCAGCCTTATAA+GGG 0.418934 3_3:-35547364 Msa0450200:three_prime_UTR
ATTAACTAAACCCTCCCATT+TGG 0.422471 3_3:-35546779 Msa0450200:three_prime_UTR
CATATTGCCAATCATTAAGA+AGG 0.425262 3_3:+35547842 None:intergenic
TTCACATCTGCCAACTTGAC+AGG 0.430476 3_3:+35547174 None:intergenic
ATGAATCAATGGTTATAGTC+TGG 0.434730 3_3:+35546990 None:intergenic
CTGACAATGAGTATAGGATT+AGG 0.443189 3_3:-35546884 Msa0450200:three_prime_UTR
ACATCTTGACCCGAATTGAT+AGG 0.448548 3_3:-35548354 Msa0450200:CDS
ATAACCATTGATTCATTTAG+CGG 0.455397 3_3:-35546983 Msa0450200:three_prime_UTR
GTGAAATATGTATGGAATAT+GGG 0.457133 3_3:-35548096 Msa0450200:three_prime_UTR
ATCCATTTGTGAAATATGTA+TGG 0.457154 3_3:-35548104 Msa0450200:three_prime_UTR
AATAATGTTGTCATGCCAGC+AGG 0.457290 3_3:+35547488 None:intergenic
GGCCATGTGTTGGTGAGAAA+AGG 0.459772 3_3:+35547710 None:intergenic
CATGCCAGCAGGGTCCTTGT+AGG 0.461652 3_3:+35547499 None:intergenic
CTTAAATTCACTTCTTGCAA+AGG 0.463478 3_3:-35548976 Msa0450200:intron
CAAATTTGATGATCCATGAA+TGG 0.469303 3_3:-35547295 Msa0450200:three_prime_UTR
TTGAAGTAGACCCTGCCAAA+TGG 0.471084 3_3:+35546764 None:intergenic
ACTAAACCCTCCCATTTGGC+AGG 0.473859 3_3:-35546775 Msa0450200:three_prime_UTR
ATTGACAGTTGCTTGGAAGA+AGG 0.475916 3_3:-35547219 Msa0450200:three_prime_UTR
TAACATGGTGATATTATTGA+GGG 0.476877 3_3:+35547617 None:intergenic
ACACCAGCACCACTTACTTC+CGG 0.479536 3_3:+35549063 None:intergenic
TTACAGTATCTTGGTTTGGA+TGG 0.481557 3_3:-35548816 Msa0450200:CDS
TGAAGTAGACCCTGCCAAAT+GGG 0.496813 3_3:+35546765 None:intergenic
CTAACATGGTGATATTATTG+AGG 0.496837 3_3:+35547616 None:intergenic
GATGGTGACACGAGTTCCAT+TGG 0.497003 3_3:-35548798 Msa0450200:CDS
AAGATCTTACCTATCAATTC+GGG 0.507592 3_3:+35548345 None:intergenic
TTTGTTCATTGACAGTTGCT+TGG 0.513588 3_3:-35547226 Msa0450200:three_prime_UTR
AATGGATTTCATGTCTAGAT+GGG 0.515419 3_3:+35548120 None:intergenic
GTTTAGGCTGGCACTGAATG+TGG 0.516455 3_3:-35547891 Msa0450200:three_prime_UTR
AGAGGTGAAGGAAAGAAAGT+CGG 0.518178 3_3:-35547129 Msa0450200:three_prime_UTR
ATATCTAATTAGGTTTAGGC+TGG 0.518777 3_3:-35547903 Msa0450200:three_prime_UTR
AAGAGAAAGAGAGAGAGATA+CGG 0.521048 3_3:+35549256 None:intergenic
AAACAATACTAATTCAAAAG+AGG 0.522029 3_3:+35548308 None:intergenic
GGCACCGCTAAATGAATCAA+TGG 0.523340 3_3:+35546979 None:intergenic
TTTGCTTACATCAAAACCAA+TGG 0.528283 3_3:+35548782 None:intergenic
TTACCGGAAGTAAGTGGTGC+TGG 0.532656 3_3:-35549066 Msa0450200:CDS
GAAGTAAGTGGTGCTGGTGT+TGG 0.533059 3_3:-35549060 Msa0450200:CDS
AAATTGCTGACAATGAGTAT+AGG 0.533650 3_3:-35546890 Msa0450200:three_prime_UTR
ATAATTTCAGGAATAAAAGT+AGG 0.534020 3_3:+35547411 None:intergenic
TTGATCGCAAAGTCATTGAA+AGG 0.535022 3_3:-35549002 Msa0450200:CDS
GCAGTTACTGTTGTTGAGAG+TGG 0.535906 3_3:-35548849 Msa0450200:intron
GCCATGTGTTGGTGAGAAAA+GGG 0.535915 3_3:+35547711 None:intergenic
AAAATACAACATAGCCTTGG+TGG 0.538703 3_3:+35547771 None:intergenic
TTGTATTTCATCTCTATCAA+CGG 0.543618 3_3:-35547070 Msa0450200:three_prime_UTR
CATGGATCATCAAATTTGCA+AGG 0.548667 3_3:+35547300 None:intergenic
TCAGAGGACATAACCACCAC+TGG 0.556667 3_3:+35548379 None:intergenic
TCTATTGAAACCTGTCAAGT+TGG 0.561378 3_3:-35547184 Msa0450200:three_prime_UTR
ATTCCCTTATAAGGCTGAGA+AGG 0.565598 3_3:+35547361 None:intergenic
TTCTTTGACGACATTGCAGA+CGG 0.571495 3_3:+35547015 None:intergenic
GGTGAAGGAAAGAAAGTCGG+AGG 0.571633 3_3:-35547126 Msa0450200:three_prime_UTR
AGAGTTGCACGTTCTTGCAG+TGG 0.580799 3_3:-35549037 Msa0450200:CDS
CCATGTGTCTAACTCTAACA+TGG 0.582722 3_3:+35547602 None:intergenic
GAAGATTACACTGTAAATAG+GGG 0.585833 3_3:+35546696 None:intergenic
TAGGATTAGGACTGATGAAG+AGG 0.592905 3_3:-35546871 Msa0450200:three_prime_UTR
ATTTAGAGAAATTCCAGTGG+TGG 0.593101 3_3:-35548392 Msa0450200:CDS
GATTTCATAATGAGAGGTGA+AGG 0.593144 3_3:-35547141 Msa0450200:three_prime_UTR
TTAACAGATTTCATAATGAG+AGG 0.593540 3_3:-35547147 Msa0450200:three_prime_UTR
GACATTGCAGACGGTGACAA+TGG 0.595350 3_3:+35547024 None:intergenic
CAGAGTTTACCGGAAGTAAG+TGG 0.596307 3_3:-35549072 Msa0450200:CDS
TTGCTTGGAAGAAGGAGCAG+AGG 0.596704 3_3:-35547211 Msa0450200:three_prime_UTR
CTAAACCCTCCCATTTGGCA+GGG 0.601382 3_3:-35546774 Msa0450200:three_prime_UTR
AATATGGTTACAGGGTGTGA+AGG 0.609461 3_3:-35548662 Msa0450200:intron
GTAGACCCTGCCAAATGGGA+GGG 0.631769 3_3:+35546769 None:intergenic
AGTAGACCCTGCCAAATGGG+AGG 0.633441 3_3:+35546768 None:intergenic
AGAGTTAGACACATGGAGTG+TGG 0.644046 3_3:-35547595 Msa0450200:three_prime_UTR
AGAAAGAGAGAGAGATACGG+AGG 0.646002 3_3:+35549259 None:intergenic
TGATAAAACCTATAATTGTG+TGG 0.657257 3_3:+35547449 None:intergenic
CCATGTTAGAGTTAGACACA+TGG 0.657291 3_3:-35547602 Msa0450200:three_prime_UTR
ATGATTATGATCACCCACCA+TGG 0.659262 3_3:+35547974 None:intergenic
AAGATTACACTGTAAATAGG+GGG 0.670911 3_3:+35546697 None:intergenic
ATAATGTTGTCATGCCAGCA+GGG 0.671199 3_3:+35547489 None:intergenic
ATATTACCAATTTACCACCA+AGG 0.677760 3_3:-35547785 Msa0450200:three_prime_UTR
TGTATTTAGAGAAATTCCAG+TGG 0.707997 3_3:-35548395 Msa0450200:CDS
TCGGGTCAAGATGTTCTCAG+AGG 0.723155 3_3:+35548363 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTTTTTTTTTTTTTG+AGG - chr3_3:35547188-35547207 Msa0450200:three_prime_UTR 10.0%
!! ACACATAAAAAAATTCAAAA+GGG + chr3_3:35546991-35547010 None:intergenic 15.0%
!! AACACATAAAAAAATTCAAA+AGG + chr3_3:35546992-35547011 None:intergenic 15.0%
!!! TGAATTTTTTTATGTGTTTT+TGG - chr3_3:35546994-35547013 Msa0450200:three_prime_UTR 15.0%
!! AAAATAAAAAAGAATTGCAT+AGG + chr3_3:35547728-35547747 None:intergenic 15.0%
!!! ATTAAAGACATGTATATTTT+TGG - chr3_3:35547766-35547785 Msa0450200:three_prime_UTR 15.0%
!!! TTTTTTTAAGCAAAAAATCG+AGG + chr3_3:35546613-35546632 None:intergenic 20.0%
!!! TTTTTGAAGAAGAAGAAAAA+TGG + chr3_3:35546708-35546727 None:intergenic 20.0%
!! TAATCTAATCTAATCTAATC+AGG + chr3_3:35547422-35547441 None:intergenic 20.0%
!! AAACAATACTAATTCAAAAG+AGG + chr3_3:35547612-35547631 None:intergenic 20.0%
!!! GATTAGTTATAGTTACATAT+TGG - chr3_3:35547684-35547703 Msa0450200:three_prime_UTR 20.0%
!! GTCTTTAATACAAAAACTAA+AGG + chr3_3:35547755-35547774 None:intergenic 20.0%
!! AAAGATCTCAATATCTAATT+AGG - chr3_3:35548004-35548023 Msa0450200:three_prime_UTR 20.0%
!! ATAATTTCAGGAATAAAAGT+AGG + chr3_3:35548509-35548528 None:intergenic 20.0%
!! ATGATCAATAAATATGAAAC+AGG + chr3_3:35548666-35548685 None:intergenic 20.0%
!! AAATATCTCAAAAAAGAAGT+CGG - chr3_3:35548816-35548835 Msa0450200:CDS 20.0%
!! TTAGAATGATGTTAAAGATT+TGG - chr3_3:35549196-35549215 Msa0450200:five_prime_UTR 20.0%
!!! TCTTATTTTACTGTCTTTTT+TGG - chr3_3:35549242-35549261 Msa0450200:five_prime_UTR 20.0%
!! AATGGCAAAGTGTTATATAA+AGG + chr3_3:35546690-35546709 None:intergenic 25.0%
!!! TTATGTGTTTTTGGTTCATT+TGG - chr3_3:35547003-35547022 Msa0450200:three_prime_UTR 25.0%
!! GATTGGATTCTAATGTTTTT+CGG - chr3_3:35547025-35547044 Msa0450200:three_prime_UTR 25.0%
!! GTAAGCAAAGTTTCTAATTT+TGG - chr3_3:35547149-35547168 Msa0450200:three_prime_UTR 25.0%
!! GTATTTTCAGTTTTTGTGTT+TGG - chr3_3:35547210-35547229 Msa0450200:three_prime_UTR 25.0%
!!! TGTTGTTGTTTTGATGAATA+TGG - chr3_3:35547239-35547258 Msa0450200:three_prime_UTR 25.0%
!! TTTTGATGAATATGGTTACA+GGG - chr3_3:35547247-35547266 Msa0450200:three_prime_UTR 25.0%
!!! TTATTTCTGTTAATTTCAGC+AGG - chr3_3:35547492-35547511 Msa0450200:three_prime_UTR 25.0%
! AATCTCAGCTAAAAAACAAT+AGG + chr3_3:35547643-35547662 None:intergenic 25.0%
! ATCCATTTGTGAAATATGTA+TGG - chr3_3:35547813-35547832 Msa0450200:three_prime_UTR 25.0%
! TTCCATACATATTTCACAAA+TGG + chr3_3:35547818-35547837 None:intergenic 25.0%
! TGTGAAATATGTATGGAATA+TGG - chr3_3:35547820-35547839 Msa0450200:three_prime_UTR 25.0%
! GTGAAATATGTATGGAATAT+GGG - chr3_3:35547821-35547840 Msa0450200:three_prime_UTR 25.0%
! TAGAATAAGAAAAAACGTGT+GGG + chr3_3:35547856-35547875 None:intergenic 25.0%
! CTCAATATCTAATTAGGTTT+AGG - chr3_3:35548010-35548029 Msa0450200:three_prime_UTR 25.0%
!!! TCATTTTAGTGAAAGGATTT+TGG + chr3_3:35548231-35548250 None:intergenic 25.0%
! AATCCTTTCACTAAAATGAT+AGG - chr3_3:35548232-35548251 Msa0450200:three_prime_UTR 25.0%
!!! TAACCTATCATTTTAGTGAA+AGG + chr3_3:35548238-35548257 None:intergenic 25.0%
! TTGCAAAATCTTTCTACTAA+TGG - chr3_3:35548266-35548285 Msa0450200:three_prime_UTR 25.0%
! TAACATGGTGATATTATTGA+GGG + chr3_3:35548303-35548322 None:intergenic 25.0%
! TATAATTGCCACACAATTAT+AGG - chr3_3:35548460-35548479 Msa0450200:intron 25.0%
! TGATAAAACCTATAATTGTG+TGG + chr3_3:35548471-35548490 None:intergenic 25.0%
! TTAACAGATTTCATAATGAG+AGG - chr3_3:35548770-35548789 Msa0450200:intron 25.0%
! TTGTATTTCATCTCTATCAA+CGG - chr3_3:35548847-35548866 Msa0450200:CDS 25.0%
!!! ATAACCATTGATTCATTTAG+CGG - chr3_3:35548934-35548953 Msa0450200:intron 25.0%
! AGAAGATTACACTGTAAATA+GGG + chr3_3:35549225-35549244 None:intergenic 25.0%
! AAGAAGATTACACTGTAAAT+AGG + chr3_3:35549226-35549245 None:intergenic 25.0%
! TTTAGATTGGAATTGTAGTT+AGG + chr3_3:35549311-35549330 None:intergenic 25.0%
!! ATGAAGAAGAAGTTTGTTGA+TGG + chr3_3:35546786-35546805 None:intergenic 30.0%
CTTAAATTCACTTCTTGCAA+AGG - chr3_3:35546941-35546960 Msa0450200:three_prime_UTR 30.0%
!!! TGTTTTTGGTTCATTTGGAT+TGG - chr3_3:35547008-35547027 Msa0450200:three_prime_UTR 30.0%
TTTGCTTACATCAAAACCAA+TGG + chr3_3:35547138-35547157 None:intergenic 30.0%
!! GTTTTGATGAATATGGTTAC+AGG - chr3_3:35547246-35547265 Msa0450200:three_prime_UTR 30.0%
TGAATTGCTCAAGAAGATTA+AGG - chr3_3:35547324-35547343 Msa0450200:three_prime_UTR 30.0%
! GATGTATGTATTGATGAAAG+AGG - chr3_3:35547390-35547409 Msa0450200:three_prime_UTR 30.0%
TGTATTTAGAGAAATTCCAG+TGG - chr3_3:35547522-35547541 Msa0450200:three_prime_UTR 30.0%
AAGATCTTACCTATCAATTC+GGG + chr3_3:35547575-35547594 None:intergenic 30.0%
CAAGATCTTACCTATCAATT+CGG + chr3_3:35547576-35547595 None:intergenic 30.0%
! AATGGATTTCATGTCTAGAT+GGG + chr3_3:35547800-35547819 None:intergenic 30.0%
! AAATGGATTTCATGTCTAGA+TGG + chr3_3:35547801-35547820 None:intergenic 30.0%
CTAGAATAAGAAAAAACGTG+TGG + chr3_3:35547857-35547876 None:intergenic 30.0%
!!! CACACGTTTTTTCTTATTCT+AGG - chr3_3:35547855-35547874 Msa0450200:three_prime_UTR 30.0%
!!! CGTTTTTTCTTATTCTAGGT+TGG - chr3_3:35547859-35547878 Msa0450200:three_prime_UTR 30.0%
ATATCTAATTAGGTTTAGGC+TGG - chr3_3:35548014-35548033 Msa0450200:three_prime_UTR 30.0%
CATTAAGCCTTCTTAATGAT+TGG - chr3_3:35548068-35548087 Msa0450200:three_prime_UTR 30.0%
CATATTGCCAATCATTAAGA+AGG + chr3_3:35548078-35548097 None:intergenic 30.0%
ATATTACCAATTTACCACCA+AGG - chr3_3:35548132-35548151 Msa0450200:three_prime_UTR 30.0%
ACAAAAATACAACATAGCCT+TGG + chr3_3:35548152-35548171 None:intergenic 30.0%
GAGAAAAGGGATTTATTGTT+TGG + chr3_3:35548196-35548215 None:intergenic 30.0%
CTAACATGGTGATATTATTG+AGG + chr3_3:35548304-35548323 None:intergenic 30.0%
! ATGAAATCCATTTTCCTACA+AGG - chr3_3:35548404-35548423 Msa0450200:CDS 30.0%
TGGTGTTTGTCAATAATTTC+AGG + chr3_3:35548521-35548540 None:intergenic 30.0%
GAAGGTTCAACATAACAAAA+TGG + chr3_3:35548541-35548560 None:intergenic 30.0%
! CAAATTTGATGATCCATGAA+TGG - chr3_3:35548622-35548641 Msa0450200:intron 30.0%
AAGTAAGAAACTTCCATTCA+TGG + chr3_3:35548638-35548657 None:intergenic 30.0%
ATGAATCAATGGTTATAGTC+TGG + chr3_3:35548930-35548949 None:intergenic 30.0%
AGCAAAAATGGAGACATAAA+GGG + chr3_3:35548962-35548981 None:intergenic 30.0%
GAACATTAGTTCAGCAAAAA+TGG + chr3_3:35548974-35548993 None:intergenic 30.0%
AAATTGCTGACAATGAGTAT+AGG - chr3_3:35549027-35549046 Msa0450200:CDS 30.0%
AAGATTACACTGTAAATAGG+GGG + chr3_3:35549223-35549242 None:intergenic 30.0%
GAAGATTACACTGTAAATAG+GGG + chr3_3:35549224-35549243 None:intergenic 30.0%
AAGAGAAAGAGAGAGAGATA+CGG + chr3_3:35546664-35546683 None:intergenic 35.0%
TTGATCGCAAAGTCATTGAA+AGG - chr3_3:35546915-35546934 Msa0450200:three_prime_UTR 35.0%
ACTAGAGCTTTACAGTATCT+TGG - chr3_3:35547092-35547111 Msa0450200:three_prime_UTR 35.0%
AGCTTTACAGTATCTTGGTT+TGG - chr3_3:35547097-35547116 Msa0450200:three_prime_UTR 35.0%
!! TTACAGTATCTTGGTTTGGA+TGG - chr3_3:35547101-35547120 Msa0450200:three_prime_UTR 35.0%
ATGACAGATTATTCTATGCC+TGG - chr3_3:35547289-35547308 Msa0450200:three_prime_UTR 35.0%
TGACAGATTATTCTATGCCT+GGG - chr3_3:35547290-35547309 Msa0450200:three_prime_UTR 35.0%
ATTTAGAGAAATTCCAGTGG+TGG - chr3_3:35547525-35547544 Msa0450200:three_prime_UTR 35.0%
!!! ATGGCTATTTTTGGATTTGG+TGG + chr3_3:35547927-35547946 None:intergenic 35.0%
ACCAAATCCAAAAATAGCCA+TGG - chr3_3:35547926-35547945 Msa0450200:three_prime_UTR 35.0%
!!! ACCATGGCTATTTTTGGATT+TGG + chr3_3:35547930-35547949 None:intergenic 35.0%
AAATCCAAAAATAGCCATGG+TGG - chr3_3:35547929-35547948 Msa0450200:three_prime_UTR 35.0%
AATCCAAAAATAGCCATGGT+GGG - chr3_3:35547930-35547949 Msa0450200:three_prime_UTR 35.0%
! AAAATACAACATAGCCTTGG+TGG + chr3_3:35548149-35548168 None:intergenic 35.0%
CATGGATCATCAAATTTGCA+AGG + chr3_3:35548620-35548639 None:intergenic 35.0%
TTTGTTCATTGACAGTTGCT+TGG - chr3_3:35548691-35548710 Msa0450200:intron 35.0%
TCTATTGAAACCTGTCAAGT+TGG - chr3_3:35548733-35548752 Msa0450200:intron 35.0%
GATTTCATAATGAGAGGTGA+AGG - chr3_3:35548776-35548795 Msa0450200:intron 35.0%
CAGCAAAAATGGAGACATAA+AGG + chr3_3:35548963-35548982 None:intergenic 35.0%
CTGACAATGAGTATAGGATT+AGG - chr3_3:35549033-35549052 Msa0450200:CDS 35.0%
ATTAACTAAACCCTCCCATT+TGG - chr3_3:35549138-35549157 Msa0450200:five_prime_UTR 35.0%
!! TGGTAATGAGTTTTGCTACA+AGG - chr3_3:35549262-35549281 Msa0450200:five_prime_UTR 35.0%
AAGCAAAAAATCGAGGACAG+AGG + chr3_3:35546606-35546625 None:intergenic 40.0%
!! TCTTCATCGCTTTTCTGTGA+TGG - chr3_3:35546799-35546818 Msa0450200:three_prime_UTR 40.0%
AATATGGTTACAGGGTGTGA+AGG - chr3_3:35547255-35547274 Msa0450200:three_prime_UTR 40.0%
AATTCATATCCTGTCATCCC+AGG + chr3_3:35547310-35547329 None:intergenic 40.0%
ACATCTTGACCCGAATTGAT+AGG - chr3_3:35547563-35547582 Msa0450200:three_prime_UTR 40.0%
ATGATTATGATCACCCACCA+TGG + chr3_3:35547946-35547965 None:intergenic 40.0%
! ACATAGCCTTGGTGGTAAAT+TGG + chr3_3:35548141-35548160 None:intergenic 40.0%
!! AAAGGATTTTGGCCATGTGT+TGG + chr3_3:35548220-35548239 None:intergenic 40.0%
CCATGTGTCTAACTCTAACA+TGG + chr3_3:35548318-35548337 None:intergenic 40.0%
CCATGTTAGAGTTAGACACA+TGG - chr3_3:35548315-35548334 Msa0450200:three_prime_UTR 40.0%
ATAATGTTGTCATGCCAGCA+GGG + chr3_3:35548431-35548450 None:intergenic 40.0%
AATAATGTTGTCATGCCAGC+AGG + chr3_3:35548432-35548451 None:intergenic 40.0%
! TGAACCTTCTCAGCCTTATA+AGG - chr3_3:35548552-35548571 Msa0450200:intron 40.0%
! GAACCTTCTCAGCCTTATAA+GGG - chr3_3:35548553-35548572 Msa0450200:intron 40.0%
ATTCCCTTATAAGGCTGAGA+AGG + chr3_3:35548559-35548578 None:intergenic 40.0%
CTCATGCACATTCCCTTATA+AGG + chr3_3:35548568-35548587 None:intergenic 40.0%
ATTGACAGTTGCTTGGAAGA+AGG - chr3_3:35548698-35548717 Msa0450200:intron 40.0%
AGAGGTGAAGGAAAGAAAGT+CGG - chr3_3:35548788-35548807 Msa0450200:intron 40.0%
TTCTTTGACGACATTGCAGA+CGG + chr3_3:35548905-35548924 None:intergenic 40.0%
TAGGATTAGGACTGATGAAG+AGG - chr3_3:35549046-35549065 Msa0450200:CDS 40.0%
AGAAAGAGAGAGAGATACGG+AGG + chr3_3:35546661-35546680 None:intergenic 45.0%
! TTTTCTGTGATGGCTACTGC+TGG - chr3_3:35546809-35546828 Msa0450200:three_prime_UTR 45.0%
TGTTCTCAGGCAGAGTTTAC+CGG - chr3_3:35546835-35546854 Msa0450200:three_prime_UTR 45.0%
CAGAGTTTACCGGAAGTAAG+TGG - chr3_3:35546845-35546864 Msa0450200:three_prime_UTR 45.0%
GCAGTTACTGTTGTTGAGAG+TGG - chr3_3:35547068-35547087 Msa0450200:three_prime_UTR 45.0%
TATTCTATGCCTGGGATGAC+AGG - chr3_3:35547298-35547317 Msa0450200:three_prime_UTR 45.0%
!! TCACCCACCATGGCTATTTT+TGG + chr3_3:35547936-35547955 None:intergenic 45.0%
! TCCCTTTTCTCACCAACACA+TGG - chr3_3:35548205-35548224 Msa0450200:three_prime_UTR 45.0%
! GCCATGTGTTGGTGAGAAAA+GGG + chr3_3:35548209-35548228 None:intergenic 45.0%
AGAGTTAGACACATGGAGTG+TGG - chr3_3:35548322-35548341 Msa0450200:three_prime_UTR 45.0%
TTCACATCTGCCAACTTGAC+AGG + chr3_3:35548746-35548765 None:intergenic 45.0%
GGCACCGCTAAATGAATCAA+TGG + chr3_3:35548941-35548960 None:intergenic 45.0%
TGAAGTAGACCCTGCCAAAT+GGG + chr3_3:35549155-35549174 None:intergenic 45.0%
TTGAAGTAGACCCTGCCAAA+TGG + chr3_3:35549156-35549175 None:intergenic 45.0%
! CTACTGCTGGTGATGTTCTC+AGG - chr3_3:35546822-35546841 Msa0450200:three_prime_UTR 50.0%
TTACCGGAAGTAAGTGGTGC+TGG - chr3_3:35546851-35546870 Msa0450200:three_prime_UTR 50.0%
ACACCAGCACCACTTACTTC+CGG + chr3_3:35546857-35546876 None:intergenic 50.0%
!! GAAGTAAGTGGTGCTGGTGT+TGG - chr3_3:35546857-35546876 Msa0450200:three_prime_UTR 50.0%
AGAGTTGCACGTTCTTGCAG+TGG - chr3_3:35546880-35546899 Msa0450200:three_prime_UTR 50.0%
GATGGTGACACGAGTTCCAT+TGG - chr3_3:35547119-35547138 Msa0450200:three_prime_UTR 50.0%
TCAGAGGACATAACCACCAC+TGG + chr3_3:35547541-35547560 None:intergenic 50.0%
TCGGGTCAAGATGTTCTCAG+AGG + chr3_3:35547557-35547576 None:intergenic 50.0%
! GTTTAGGCTGGCACTGAATG+TGG - chr3_3:35548026-35548045 Msa0450200:three_prime_UTR 50.0%
! GGCCATGTGTTGGTGAGAAA+AGG + chr3_3:35548210-35548229 None:intergenic 50.0%
GCAGGGTCCTTGTAGGAAAA+TGG + chr3_3:35548414-35548433 None:intergenic 50.0%
! TTTTCCTACAAGGACCCTGC+TGG - chr3_3:35548414-35548433 Msa0450200:intron 50.0%
TTGCTTGGAAGAAGGAGCAG+AGG - chr3_3:35548706-35548725 Msa0450200:intron 50.0%
GGTGAAGGAAAGAAAGTCGG+AGG - chr3_3:35548791-35548810 Msa0450200:CDS 50.0%
GACATTGCAGACGGTGACAA+TGG + chr3_3:35548896-35548915 None:intergenic 50.0%
ACTAAACCCTCCCATTTGGC+AGG - chr3_3:35549142-35549161 Msa0450200:five_prime_UTR 50.0%
CTAAACCCTCCCATTTGGCA+GGG - chr3_3:35549143-35549162 Msa0450200:five_prime_UTR 50.0%
GTAGACCCTGCCAAATGGGA+GGG + chr3_3:35549151-35549170 None:intergenic 55.0%
AGTAGACCCTGCCAAATGGG+AGG + chr3_3:35549152-35549171 None:intergenic 55.0%
CATGCCAGCAGGGTCCTTGT+AGG + chr3_3:35548421-35548440 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 35546597 35549342 35546597 ID=Msa0450200;Name=Msa0450200
chr3_3 mRNA 35546597 35549342 35546597 ID=Msa0450200-mRNA-1;Parent=Msa0450200;Name=Msa0450200-mRNA-1;_AED=0.05;_eAED=0.06;_QI=221|0.5|0.6|1|1|1|5|1754|104
chr3_3 exon 35546597 35548425 35546597 ID=Msa0450200-mRNA-1:exon:6317;Parent=Msa0450200-mRNA-1
chr3_3 exon 35548547 35548555 35548547 ID=Msa0450200-mRNA-1:exon:6316;Parent=Msa0450200-mRNA-1
chr3_3 exon 35548663 35548671 35548663 ID=Msa0450200-mRNA-1:exon:6315;Parent=Msa0450200-mRNA-1
chr3_3 exon 35548791 35548867 35548791 ID=Msa0450200-mRNA-1:exon:6314;Parent=Msa0450200-mRNA-1
chr3_3 exon 35548977 35549342 35548977 ID=Msa0450200-mRNA-1:exon:6313;Parent=Msa0450200-mRNA-1
chr3_3 five_prime_UTR 35549122 35549342 35549122 ID=Msa0450200-mRNA-1:five_prime_utr;Parent=Msa0450200-mRNA-1
chr3_3 CDS 35548977 35549121 35548977 ID=Msa0450200-mRNA-1:cds;Parent=Msa0450200-mRNA-1
chr3_3 CDS 35548791 35548867 35548791 ID=Msa0450200-mRNA-1:cds;Parent=Msa0450200-mRNA-1
chr3_3 CDS 35548663 35548671 35548663 ID=Msa0450200-mRNA-1:cds;Parent=Msa0450200-mRNA-1
chr3_3 CDS 35548547 35548555 35548547 ID=Msa0450200-mRNA-1:cds;Parent=Msa0450200-mRNA-1
chr3_3 CDS 35548351 35548425 35548351 ID=Msa0450200-mRNA-1:cds;Parent=Msa0450200-mRNA-1
chr3_3 three_prime_UTR 35546597 35548350 35546597 ID=Msa0450200-mRNA-1:three_prime_utr;Parent=Msa0450200-mRNA-1
Gene Sequence

>Msa0450200

ATGGCTACTGCTGGTGATGTTCTCAGGCAGAGTTTACCGGAAGTAAGTGGTGCTGGTGTTGGAGAGTTGCACGTTCTTGCAGTGGATGATAGTTTTGTTGATCGCAAAGTCATTGAAAGGTTGCTTAAATTCACTTCTTGCAAAGTTACTGTTGTTGAGAGTGGAACTAGAGCTTTACAGTATCTTGGTTTGGATGGTGACACGAGTTCCATTGGTTTTGATGGTGTGAAGATTAGAGATGAATCATCTGTATTTAGAGAAATTCCAGTGGTGGTTATGTCCTCTGAGAACATCTTGACCCGAATTGATAGGTAA
Protein sequence

>Msa0450200

MATAGDVLRQSLPEVSGAGVGELHVLAVDDSFVDRKVIERLLKFTSCKVTVVESGTRALQYLGLDGDTSSIGFDGVKIRDESSVFREIPVVVMSSENILTRIDR*