AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0511640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0511640 MtrunA17_Chr3g0118211 97.015 201 5 1 1 201 1 200 1.31e-138 385
Msa0511640 MtrunA17_Chr4g0059571 68.519 216 51 6 1 201 1 214 1.49e-87 256
Msa0511640 MtrunA17_Chr1g0170741 61.290 155 53 3 22 173 7 157 2.11e-58 182
Msa0511640 MtrunA17_Chr5g0414931 65.035 143 45 2 17 155 10 151 1.31e-57 181
Msa0511640 MtrunA17_Chr7g0256551 52.761 163 67 3 18 171 4 165 9.78e-53 169
Msa0511640 MtrunA17_Chr3g0082861 51.829 164 69 3 20 174 1 163 2.21e-50 160
Msa0511640 MtrunA17_Chr3g0128691 58.647 133 50 2 24 153 19 149 1.08e-49 158
Msa0511640 MtrunA17_Chr8g0352871 54.667 150 57 3 18 158 4 151 8.59e-49 157
Msa0511640 MtrunA17_Chr3g0124861 50.667 150 54 2 22 153 21 168 2.75e-46 152
Msa0511640 MtrunA17_Chr4g0021731 54.651 86 39 0 15 100 3 88 1.55e-25 94.7
Msa0511640 MtrunA17_Chr3g0127941 28.859 149 96 3 23 171 56 194 1.18e-13 68.9
Msa0511640 MtrunA17_Chr1g0149851 29.114 158 96 4 23 180 9 150 3.38e-12 64.7
Msa0511640 MtrunA17_Chr4g0030271 30.597 134 83 2 20 153 86 209 5.58e-12 63.9
Msa0511640 MtrunA17_Chr8g0345901 28.682 129 82 2 25 153 38 156 6.94e-12 63.5
Msa0511640 MtrunA17_Chr7g0275401 29.530 149 85 3 23 171 9 137 2.10e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0511640 AT3G48100.1 63.736 182 59 3 5 180 2 182 1.98e-75 225
Msa0511640 AT1G74890.1 75.333 150 30 3 22 167 17 163 1.11e-71 216
Msa0511640 AT5G62920.1 74.483 145 35 2 23 167 25 167 1.57e-70 213
Msa0511640 AT1G19050.1 67.089 158 49 2 3 158 2 158 3.48e-69 210
Msa0511640 AT1G59940.1 62.857 175 45 5 1 158 1 172 1.94e-66 204
Msa0511640 AT1G59940.2 62.857 175 45 5 1 158 93 264 3.86e-65 204
Msa0511640 AT1G10470.3 66.443 149 49 1 14 162 11 158 2.13e-63 196
Msa0511640 AT1G10470.1 72.727 132 35 1 22 153 33 163 3.63e-63 196
Msa0511640 AT2G41310.1 56.667 150 56 3 21 162 7 155 3.92e-53 170
Msa0511640 AT3G57040.2 57.534 146 51 2 18 153 4 148 4.16e-53 170
Msa0511640 AT3G57040.1 57.534 146 51 2 18 153 4 148 4.16e-53 170
Msa0511640 AT2G40670.2 59.559 136 50 2 22 153 28 162 5.06e-53 167
Msa0511640 AT3G56380.2 58.647 133 54 1 22 153 19 151 1.09e-52 166
Msa0511640 AT2G40670.1 58.824 136 50 3 22 153 28 161 3.00e-50 160
Msa0511640 AT3G56380.1 57.895 133 54 2 22 153 19 150 6.08e-50 159
Msa0511640 AT1G10470.2 61.111 72 28 0 91 162 25 96 8.06e-23 90.9
Msa0511640 AT2G07440.1 52.941 68 32 0 95 162 2 69 1.16e-16 73.6
Msa0511640 AT5G07210.2 30.508 177 97 6 1 175 1 153 6.84e-15 72.8
Msa0511640 AT5G24470.1 29.771 131 82 3 23 153 50 170 2.61e-12 65.5
Msa0511640 AT1G67710.1 30.137 146 89 3 14 159 2 134 1.08e-11 63.5
Msa0511640 AT2G46790.3 27.481 131 85 2 23 153 37 157 1.83e-11 62.8
Msa0511640 AT2G46790.1 27.481 131 85 2 23 153 37 157 2.14e-11 62.4
Msa0511640 AT5G60100.7 31.343 134 82 3 20 153 61 184 6.77e-11 60.8
Msa0511640 AT5G60100.6 31.343 134 82 3 20 153 61 184 6.77e-11 60.8
Msa0511640 AT5G60100.4 31.343 134 82 3 20 153 61 184 6.77e-11 60.8
Msa0511640 AT5G60100.2 31.343 134 82 3 20 153 61 184 7.32e-11 60.8

Find 61 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGATTACACTGTAAAT+AGG 0.246511 3_4:+61238151 None:intergenic
TTAGAATGATGTTAAAGATT+TGG 0.295608 3_4:-61238178 Msa0511640:three_prime_UTR
AGAAGATTACACTGTAAATA+GGG 0.323672 3_4:+61238152 None:intergenic
CAAGATCTTACCTATCAATT+CGG 0.347926 3_4:+61239788 None:intergenic
TTTAGATTGGAATTGTAGTT+AGG 0.349100 3_4:+61238066 None:intergenic
TGAATTGCTCAAGAAGATTA+AGG 0.356380 3_4:-61240027 Msa0511640:intron
ACTAGAGCTTTACAGTATCT+TGG 0.363036 3_4:-61240257 Msa0511640:CDS
AGCTTTACAGTATCTTGGTT+TGG 0.366392 3_4:-61240252 Msa0511640:CDS
CTACTGCTGGTGATGTTCTC+AGG 0.387823 3_4:-61240526 Msa0511640:CDS
AATGGCAAAGTGTTATATAA+AGG 0.400670 3_4:+61240661 None:intergenic
TGTTCTCAGGCAGAGTTTAC+CGG 0.414947 3_4:-61240513 Msa0511640:CDS
ATGAAGAAGAAGTTTGTTGA+TGG 0.417706 3_4:+61240565 None:intergenic
ATTAACTAAACCCTCCCATT+TGG 0.422471 3_4:-61238236 Msa0511640:three_prime_UTR
TTCACATCTGCCAACTTGAC+AGG 0.430476 3_4:+61238631 None:intergenic
ATGAATCAATGGTTATAGTC+TGG 0.434730 3_4:+61238447 None:intergenic
CTGACAATGAGTATAGGATT+AGG 0.443189 3_4:-61238341 Msa0511640:three_prime_UTR
ACATCTTGACCCGAATTGAT+AGG 0.448548 3_4:-61239798 Msa0511640:intron
ATGACAGATTATTCTATGCC+TGG 0.452458 3_4:-61240062 Msa0511640:CDS
TATTCTATGCCTGGGATGAC+AGG 0.453139 3_4:-61240053 Msa0511640:CDS
ATAACCATTGATTCATTTAG+CGG 0.455397 3_4:-61238440 Msa0511640:exon
CTTAAATTCACTTCTTGCAA+AGG 0.463478 3_4:-61240407 Msa0511640:intron
TTGAAGTAGACCCTGCCAAA+TGG 0.471084 3_4:+61238221 None:intergenic
ACTAAACCCTCCCATTTGGC+AGG 0.473859 3_4:-61238232 Msa0511640:three_prime_UTR
ATTGACAGTTGCTTGGAAGA+AGG 0.475916 3_4:-61238676 Msa0511640:intron
ACACCAGCACCACTTACTTC+CGG 0.479536 3_4:+61240494 None:intergenic
TTACAGTATCTTGGTTTGGA+TGG 0.481557 3_4:-61240248 Msa0511640:CDS
TGAAGTAGACCCTGCCAAAT+GGG 0.496813 3_4:+61238222 None:intergenic
GATGGTGACACGAGTTCCAT+TGG 0.497003 3_4:-61240230 Msa0511640:CDS
AAGATCTTACCTATCAATTC+GGG 0.507592 3_4:+61239789 None:intergenic
TTTGTTCATTGACAGTTGCT+TGG 0.513588 3_4:-61238683 Msa0511640:intron
AGAGGTGAAGGAAAGAAAGT+CGG 0.518178 3_4:-61238586 Msa0511640:CDS
GGCACCGCTAAATGAATCAA+TGG 0.523340 3_4:+61238436 None:intergenic
TTTGCTTACATCAAAACCAA+TGG 0.528283 3_4:+61240214 None:intergenic
TTACCGGAAGTAAGTGGTGC+TGG 0.532656 3_4:-61240497 Msa0511640:CDS
GAAGTAAGTGGTGCTGGTGT+TGG 0.533059 3_4:-61240491 Msa0511640:CDS
AAATTGCTGACAATGAGTAT+AGG 0.533650 3_4:-61238347 Msa0511640:three_prime_UTR
TTGATCGCAAAGTCATTGAA+AGG 0.535022 3_4:-61240433 Msa0511640:CDS
GCAGTTACTGTTGTTGAGAG+TGG 0.535906 3_4:-61240281 Msa0511640:intron
AATTCATATCCTGTCATCCC+AGG 0.536075 3_4:+61240044 None:intergenic
TTGTATTTCATCTCTATCAA+CGG 0.543618 3_4:-61238527 Msa0511640:CDS
TGACAGATTATTCTATGCCT+GGG 0.547632 3_4:-61240061 Msa0511640:CDS
TCAGAGGACATAACCACCAC+TGG 0.556667 3_4:+61239823 None:intergenic
TCTATTGAAACCTGTCAAGT+TGG 0.561378 3_4:-61238641 Msa0511640:CDS
TTCTTTGACGACATTGCAGA+CGG 0.571495 3_4:+61238472 None:intergenic
GGTGAAGGAAAGAAAGTCGG+AGG 0.571633 3_4:-61238583 Msa0511640:CDS
AGAGTTGCACGTTCTTGCAG+TGG 0.580799 3_4:-61240468 Msa0511640:CDS
GAAGATTACACTGTAAATAG+GGG 0.585833 3_4:+61238153 None:intergenic
TAGGATTAGGACTGATGAAG+AGG 0.592905 3_4:-61238328 Msa0511640:three_prime_UTR
ATTTAGAGAAATTCCAGTGG+TGG 0.593101 3_4:-61239836 Msa0511640:CDS
GATTTCATAATGAGAGGTGA+AGG 0.593144 3_4:-61238598 Msa0511640:CDS
TTAACAGATTTCATAATGAG+AGG 0.593540 3_4:-61238604 Msa0511640:CDS
GACATTGCAGACGGTGACAA+TGG 0.595350 3_4:+61238481 None:intergenic
CAGAGTTTACCGGAAGTAAG+TGG 0.596307 3_4:-61240503 Msa0511640:CDS
TTGCTTGGAAGAAGGAGCAG+AGG 0.596704 3_4:-61238668 Msa0511640:CDS
CTAAACCCTCCCATTTGGCA+GGG 0.601382 3_4:-61238231 Msa0511640:three_prime_UTR
AATATGGTTACAGGGTGTGA+AGG 0.609461 3_4:-61240096 Msa0511640:intron
GTAGACCCTGCCAAATGGGA+GGG 0.631769 3_4:+61238226 None:intergenic
AGTAGACCCTGCCAAATGGG+AGG 0.633441 3_4:+61238225 None:intergenic
AAGATTACACTGTAAATAGG+GGG 0.670911 3_4:+61238154 None:intergenic
TGTATTTAGAGAAATTCCAG+TGG 0.707997 3_4:-61239839 Msa0511640:CDS
TCGGGTCAAGATGTTCTCAG+AGG 0.723155 3_4:+61239807 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACACATAAAAAAATTCAAAA+GGG + chr3_4:61238449-61238468 None:intergenic 15.0%
!! AACACATAAAAAAATTCAAA+AGG + chr3_4:61238450-61238469 None:intergenic 15.0%
!!! TGAATTTTTTTATGTGTTTT+TGG - chr3_4:61238452-61238471 Msa0511640:exon 15.0%
!!! TGTGTTTTTTTTTTTTTTTG+AGG - chr3_4:61238643-61238662 Msa0511640:CDS 15.0%
!! AAAAAATAAAAGAATTGCAT+AGG + chr3_4:61239165-61239184 None:intergenic 15.0%
!!! ATTAAAGACATGTATATTTT+TGG - chr3_4:61239201-61239220 Msa0511640:intron 15.0%
!!! TTTTTTTAAGCAAAAAATCG+AGG + chr3_4:61238070-61238089 None:intergenic 20.0%
!!! TTTTTGAAGAAGAAGAAAAA+TGG + chr3_4:61238166-61238185 None:intergenic 20.0%
!! AAACAATACTAATTCAAAAG+AGG + chr3_4:61239057-61239076 None:intergenic 20.0%
!!! GATTAGTTATAGTTACATAT+TGG - chr3_4:61239121-61239140 Msa0511640:intron 20.0%
!! GTCTTTAATACAAAAACTAA+AGG + chr3_4:61239190-61239209 None:intergenic 20.0%
!! AAAGATCTCAATATCTAATT+AGG - chr3_4:61239440-61239459 Msa0511640:intron 20.0%
!! ATAATTTCAGGAATAAAAGT+AGG + chr3_4:61239945-61239964 None:intergenic 20.0%
!! ATGATCAATAAATATGAAAC+AGG + chr3_4:61240098-61240117 None:intergenic 20.0%
!! AAATATCTCAAAAAAGAAGT+CGG - chr3_4:61240248-61240267 Msa0511640:CDS 20.0%
!! TTAGAATGATGTTAAAGATT+TGG - chr3_4:61240628-61240647 Msa0511640:five_prime_UTR 20.0%
!!! TCTTATTTTACTGTCTTTTT+TGG - chr3_4:61240674-61240693 Msa0511640:five_prime_UTR 20.0%
!! AATGGCAAAGTGTTATATAA+AGG + chr3_4:61238148-61238167 None:intergenic 25.0%
!!! TTATGTGTTTTTGGTTCATT+TGG - chr3_4:61238461-61238480 Msa0511640:CDS 25.0%
!! GATTGGATTCTAATGTTTTT+CGG - chr3_4:61238483-61238502 Msa0511640:CDS 25.0%
!! GTAAGCAAAGTTTCTAATTT+TGG - chr3_4:61238606-61238625 Msa0511640:CDS 25.0%
!! GTATTTTCAGTTTTTGTGTT+TGG - chr3_4:61238665-61238684 Msa0511640:CDS 25.0%
!!! TGTTGTTGTTTTGATGAATA+TGG - chr3_4:61238694-61238713 Msa0511640:intron 25.0%
!! TTTTGATGAATATGGTTACA+GGG - chr3_4:61238702-61238721 Msa0511640:intron 25.0%
! CAATCTAATCTAATCTAATC+AGG + chr3_4:61238877-61238896 None:intergenic 25.0%
!!! TTATTTCTGTTAATTTCAGC+AGG - chr3_4:61238937-61238956 Msa0511640:intron 25.0%
! AATCTCAGCTAAAAAACAAT+AGG + chr3_4:61239088-61239107 None:intergenic 25.0%
!! TCCATTTTGTGAAATATGTA+TGG - chr3_4:61239249-61239268 Msa0511640:intron 25.0%
! ACCATACATATTTCACAAAA+TGG + chr3_4:61239253-61239272 None:intergenic 25.0%
! TGTGAAATATGTATGGTATA+TGG - chr3_4:61239256-61239275 Msa0511640:intron 25.0%
! GTGAAATATGTATGGTATAT+GGG - chr3_4:61239257-61239276 Msa0511640:intron 25.0%
! TAGAATAAGAAAAAACGTGT+GGG + chr3_4:61239292-61239311 None:intergenic 25.0%
! CTCAATATCTAATTAGGTTT+AGG - chr3_4:61239446-61239465 Msa0511640:intron 25.0%
!!! TCATTTTAGTGAAAGGATTT+TGG + chr3_4:61239667-61239686 None:intergenic 25.0%
! AATCCTTTCACTAAAATGAT+AGG - chr3_4:61239668-61239687 Msa0511640:intron 25.0%
!!! TAACCTATCATTTTAGTGAA+AGG + chr3_4:61239674-61239693 None:intergenic 25.0%
! TTGCAAAATCTTTCTACTAA+TGG - chr3_4:61239702-61239721 Msa0511640:intron 25.0%
! TAACATGGTGATATTATTGA+GGG + chr3_4:61239739-61239758 None:intergenic 25.0%
! TATAATTGCCACACAATTAT+AGG - chr3_4:61239896-61239915 Msa0511640:intron 25.0%
! TGATAAAACCTATAATTGTG+TGG + chr3_4:61239907-61239926 None:intergenic 25.0%
! TTAACAGATTTCATAATGAG+AGG - chr3_4:61240202-61240221 Msa0511640:intron 25.0%
! TTGTATTTCATCTCTATCAA+CGG - chr3_4:61240279-61240298 Msa0511640:CDS 25.0%
!!! ATAACCATTGATTCATTTAG+CGG - chr3_4:61240366-61240385 Msa0511640:intron 25.0%
! AGAAGATTACACTGTAAATA+GGG + chr3_4:61240657-61240676 None:intergenic 25.0%
! AAGAAGATTACACTGTAAAT+AGG + chr3_4:61240658-61240677 None:intergenic 25.0%
! TTTAGATTGGAATTGTAGTT+AGG + chr3_4:61240743-61240762 None:intergenic 25.0%
!! ATGAAGAAGAAGTTTGTTGA+TGG + chr3_4:61238244-61238263 None:intergenic 30.0%
CTTAAATTCACTTCTTGCAA+AGG - chr3_4:61238399-61238418 Msa0511640:three_prime_UTR 30.0%
!!! TGTTTTTGGTTCATTTGGAT+TGG - chr3_4:61238466-61238485 Msa0511640:CDS 30.0%
TTTGCTTACATCAAAACCAA+TGG + chr3_4:61238595-61238614 None:intergenic 30.0%
!! GTTTTGATGAATATGGTTAC+AGG - chr3_4:61238701-61238720 Msa0511640:intron 30.0%
TGAATTGCTCAAGAAGATTA+AGG - chr3_4:61238779-61238798 Msa0511640:intron 30.0%
! GATGTATGTATTGATGAAAG+AGG - chr3_4:61238845-61238864 Msa0511640:intron 30.0%
TGTATTTAGAGAAATTCCAG+TGG - chr3_4:61238967-61238986 Msa0511640:intron 30.0%
AAGATCTTACCTATCAATTC+GGG + chr3_4:61239020-61239039 None:intergenic 30.0%
CAAGATCTTACCTATCAATT+CGG + chr3_4:61239021-61239040 None:intergenic 30.0%
! AATGGATTTCATGTCTAGAT+GGG + chr3_4:61239235-61239254 None:intergenic 30.0%
! AAATGGATTTCATGTCTAGA+TGG + chr3_4:61239236-61239255 None:intergenic 30.0%
CTAGAATAAGAAAAAACGTG+TGG + chr3_4:61239293-61239312 None:intergenic 30.0%
!!! CACACGTTTTTTCTTATTCT+AGG - chr3_4:61239291-61239310 Msa0511640:intron 30.0%
!!! CGTTTTTTCTTATTCTAGGT+TGG - chr3_4:61239295-61239314 Msa0511640:intron 30.0%
ATATCTAATTAGGTTTAGGC+TGG - chr3_4:61239450-61239469 Msa0511640:intron 30.0%
CATTAAGCCTTCTTAATGAT+TGG - chr3_4:61239504-61239523 Msa0511640:intron 30.0%
CATATTGCCAATCATTAAGA+AGG + chr3_4:61239514-61239533 None:intergenic 30.0%
ATATTACCAATTTACCACCA+AGG - chr3_4:61239568-61239587 Msa0511640:intron 30.0%
ACAAAAATACAACATAGCCT+TGG + chr3_4:61239588-61239607 None:intergenic 30.0%
GAGAAAAGGGATTTATTGTT+TGG + chr3_4:61239632-61239651 None:intergenic 30.0%
CTAACATGGTGATATTATTG+AGG + chr3_4:61239740-61239759 None:intergenic 30.0%
! ATGAAATCCATTTTCCTACA+AGG - chr3_4:61239840-61239859 Msa0511640:CDS 30.0%
TGGTGTTTGTCAATAATTTC+AGG + chr3_4:61239957-61239976 None:intergenic 30.0%
GAAGGTTCAACATAACAAAA+TGG + chr3_4:61239977-61239996 None:intergenic 30.0%
! CAAATTTGATGATCCATGAA+TGG - chr3_4:61240054-61240073 Msa0511640:CDS 30.0%
AAGTAAGAAACTTCCATTCA+TGG + chr3_4:61240070-61240089 None:intergenic 30.0%
ATGAATCAATGGTTATAGTC+TGG + chr3_4:61240362-61240381 None:intergenic 30.0%
AGCAAAAATGGAGACATAAA+GGG + chr3_4:61240394-61240413 None:intergenic 30.0%
GAACATTAGTTCAGCAAAAA+TGG + chr3_4:61240406-61240425 None:intergenic 30.0%
AAATTGCTGACAATGAGTAT+AGG - chr3_4:61240459-61240478 Msa0511640:CDS 30.0%
AAGATTACACTGTAAATAGG+GGG + chr3_4:61240655-61240674 None:intergenic 30.0%
GAAGATTACACTGTAAATAG+GGG + chr3_4:61240656-61240675 None:intergenic 30.0%
TTGATCGCAAAGTCATTGAA+AGG - chr3_4:61238373-61238392 Msa0511640:three_prime_UTR 35.0%
ACTAGAGCTTTACAGTATCT+TGG - chr3_4:61238549-61238568 Msa0511640:CDS 35.0%
AGCTTTACAGTATCTTGGTT+TGG - chr3_4:61238554-61238573 Msa0511640:CDS 35.0%
!! TTACAGTATCTTGGTTTGGA+TGG - chr3_4:61238558-61238577 Msa0511640:CDS 35.0%
ATGACAGATTATTCTATGCC+TGG - chr3_4:61238744-61238763 Msa0511640:intron 35.0%
TGACAGATTATTCTATGCCT+GGG - chr3_4:61238745-61238764 Msa0511640:intron 35.0%
ATTTAGAGAAATTCCAGTGG+TGG - chr3_4:61238970-61238989 Msa0511640:intron 35.0%
!!! ATGGCTATTTTTGGATTTGG+TGG + chr3_4:61239363-61239382 None:intergenic 35.0%
ACCAAATCCAAAAATAGCCA+TGG - chr3_4:61239362-61239381 Msa0511640:intron 35.0%
!!! ACCATGGCTATTTTTGGATT+TGG + chr3_4:61239366-61239385 None:intergenic 35.0%
AAATCCAAAAATAGCCATGG+TGG - chr3_4:61239365-61239384 Msa0511640:intron 35.0%
AATCCAAAAATAGCCATGGT+GGG - chr3_4:61239366-61239385 Msa0511640:intron 35.0%
! AAAATACAACATAGCCTTGG+TGG + chr3_4:61239585-61239604 None:intergenic 35.0%
CATGGATCATCAAATTTGCA+AGG + chr3_4:61240052-61240071 None:intergenic 35.0%
TTTGTTCATTGACAGTTGCT+TGG - chr3_4:61240123-61240142 Msa0511640:intron 35.0%
TCTATTGAAACCTGTCAAGT+TGG - chr3_4:61240165-61240184 Msa0511640:intron 35.0%
GATTTCATAATGAGAGGTGA+AGG - chr3_4:61240208-61240227 Msa0511640:intron 35.0%
CAGCAAAAATGGAGACATAA+AGG + chr3_4:61240395-61240414 None:intergenic 35.0%
CTGACAATGAGTATAGGATT+AGG - chr3_4:61240465-61240484 Msa0511640:CDS 35.0%
ATTAACTAAACCCTCCCATT+TGG - chr3_4:61240570-61240589 Msa0511640:five_prime_UTR 35.0%
!! TGGTAATGAGTTTTGCTACA+AGG - chr3_4:61240694-61240713 Msa0511640:five_prime_UTR 35.0%
AAGCAAAAAATCGAGGACAG+AGG + chr3_4:61238063-61238082 None:intergenic 40.0%
AGAGAAGAGAGAGAGAGATA+CGG + chr3_4:61238121-61238140 None:intergenic 40.0%
!! TCTTCATCGCTTTTCTGTGA+TGG - chr3_4:61238257-61238276 Msa0511640:three_prime_UTR 40.0%
AATATGGTTACAGGGTGTGA+AGG - chr3_4:61238710-61238729 Msa0511640:intron 40.0%
AATTCATATCCTGTCATCCC+AGG + chr3_4:61238765-61238784 None:intergenic 40.0%
ACATCTTGACCCGAATTGAT+AGG - chr3_4:61239008-61239027 Msa0511640:intron 40.0%
ATGATTATGATCACCCACCA+TGG + chr3_4:61239382-61239401 None:intergenic 40.0%
! ACATAGCCTTGGTGGTAAAT+TGG + chr3_4:61239577-61239596 None:intergenic 40.0%
!! AAAGGATTTTGGCCATGTGT+TGG + chr3_4:61239656-61239675 None:intergenic 40.0%
CCATGTGTCTAACTCTAACA+TGG + chr3_4:61239754-61239773 None:intergenic 40.0%
CCATGTTAGAGTTAGACACA+TGG - chr3_4:61239751-61239770 Msa0511640:intron 40.0%
ATAATGTTGTCATGCCAGCA+GGG + chr3_4:61239867-61239886 None:intergenic 40.0%
AATAATGTTGTCATGCCAGC+AGG + chr3_4:61239868-61239887 None:intergenic 40.0%
! TGAACCTTCTCAGCCTTATA+AGG - chr3_4:61239988-61240007 Msa0511640:intron 40.0%
! GAACCTTCTCAGCCTTATAA+GGG - chr3_4:61239989-61240008 Msa0511640:intron 40.0%
ATTCCCTTATAAGGCTGAGA+AGG + chr3_4:61239995-61240014 None:intergenic 40.0%
ACTACGCACATTCCCTTATA+AGG + chr3_4:61240004-61240023 None:intergenic 40.0%
ATTGACAGTTGCTTGGAAGA+AGG - chr3_4:61240130-61240149 Msa0511640:intron 40.0%
AGAGGTGAAGGAAAGAAAGT+CGG - chr3_4:61240220-61240239 Msa0511640:intron 40.0%
TTCTTTGACGACATTGCAGA+CGG + chr3_4:61240337-61240356 None:intergenic 40.0%
TAGGATTAGGACTGATGAAG+AGG - chr3_4:61240478-61240497 Msa0511640:CDS 40.0%
! TTTTCTGTGATGGCTACTGC+TGG - chr3_4:61238267-61238286 Msa0511640:three_prime_UTR 45.0%
TGTTCTCAGGCAGAGTTTAC+CGG - chr3_4:61238293-61238312 Msa0511640:three_prime_UTR 45.0%
CAGAGTTTACCGGAAGTAAG+TGG - chr3_4:61238303-61238322 Msa0511640:three_prime_UTR 45.0%
GCAGTTACTGTTGTTGAGAG+TGG - chr3_4:61238525-61238544 Msa0511640:CDS 45.0%
TATTCTATGCCTGGGATGAC+AGG - chr3_4:61238753-61238772 Msa0511640:intron 45.0%
!! TCACCCACCATGGCTATTTT+TGG + chr3_4:61239372-61239391 None:intergenic 45.0%
! TCCCTTTTCTCACCAACACA+TGG - chr3_4:61239641-61239660 Msa0511640:intron 45.0%
! GCCATGTGTTGGTGAGAAAA+GGG + chr3_4:61239645-61239664 None:intergenic 45.0%
AGAGTTAGACACATGGAGTG+TGG - chr3_4:61239758-61239777 Msa0511640:intron 45.0%
TTCACATCTGCCAACTTGAC+AGG + chr3_4:61240178-61240197 None:intergenic 45.0%
GGCACCGCTAAATGAATCAA+TGG + chr3_4:61240373-61240392 None:intergenic 45.0%
TGAAGTAGACCCTGCCAAAT+GGG + chr3_4:61240587-61240606 None:intergenic 45.0%
TTGAAGTAGACCCTGCCAAA+TGG + chr3_4:61240588-61240607 None:intergenic 45.0%
GAAGAGAGAGAGAGATACGG+AGG + chr3_4:61238118-61238137 None:intergenic 50.0%
! CTACTGCTGGTGATGTTCTC+AGG - chr3_4:61238280-61238299 Msa0511640:three_prime_UTR 50.0%
TTACCGGAAGTAAGTGGTGC+TGG - chr3_4:61238309-61238328 Msa0511640:three_prime_UTR 50.0%
ACACCAGCACCACTTACTTC+CGG + chr3_4:61238315-61238334 None:intergenic 50.0%
!! GAAGTAAGTGGTGCTGGTGT+TGG - chr3_4:61238315-61238334 Msa0511640:three_prime_UTR 50.0%
AGAGTTGCACGTTCTTGCAG+TGG - chr3_4:61238338-61238357 Msa0511640:three_prime_UTR 50.0%
GATGGTGACACGAGTTCCAT+TGG - chr3_4:61238576-61238595 Msa0511640:CDS 50.0%
TCAGAGGACATAACCACCAC+TGG + chr3_4:61238986-61239005 None:intergenic 50.0%
TCGGGTCAAGATGTTCTCAG+AGG + chr3_4:61239002-61239021 None:intergenic 50.0%
! GTTTAGGCTGGCACTGAATG+TGG - chr3_4:61239462-61239481 Msa0511640:intron 50.0%
! GGCCATGTGTTGGTGAGAAA+AGG + chr3_4:61239646-61239665 None:intergenic 50.0%
GCAGGGTCCTTGTAGGAAAA+TGG + chr3_4:61239850-61239869 None:intergenic 50.0%
! TTTTCCTACAAGGACCCTGC+TGG - chr3_4:61239850-61239869 Msa0511640:CDS 50.0%
TTGCTTGGAAGAAGGAGCAG+AGG - chr3_4:61240138-61240157 Msa0511640:intron 50.0%
GGTGAAGGAAAGAAAGTCGG+AGG - chr3_4:61240223-61240242 Msa0511640:CDS 50.0%
GACATTGCAGACGGTGACAA+TGG + chr3_4:61240328-61240347 None:intergenic 50.0%
ACTAAACCCTCCCATTTGGC+AGG - chr3_4:61240574-61240593 Msa0511640:five_prime_UTR 50.0%
CTAAACCCTCCCATTTGGCA+GGG - chr3_4:61240575-61240594 Msa0511640:five_prime_UTR 50.0%
GTAGACCCTGCCAAATGGGA+GGG + chr3_4:61240583-61240602 None:intergenic 55.0%
AGTAGACCCTGCCAAATGGG+AGG + chr3_4:61240584-61240603 None:intergenic 55.0%
CATGCCAGCAGGGTCCTTGT+AGG + chr3_4:61239857-61239876 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 61238054 61240774 61238054 ID=Msa0511640;Name=Msa0511640
chr3_4 mRNA 61238054 61240774 61238054 ID=Msa0511640-mRNA-1;Parent=Msa0511640;Name=Msa0511640-mRNA-1;_AED=0.26;_eAED=0.26;_QI=222|0.75|0.8|1|1|1|5|405|201
chr3_4 exon 61238054 61238690 61238054 ID=Msa0511640-mRNA-1:exon:14896;Parent=Msa0511640-mRNA-1
chr3_4 exon 61239799 61239872 61239799 ID=Msa0511640-mRNA-1:exon:14895;Parent=Msa0511640-mRNA-1
chr3_4 exon 61240028 61240105 61240028 ID=Msa0511640-mRNA-1:exon:14894;Parent=Msa0511640-mRNA-1
chr3_4 exon 61240223 61240299 61240223 ID=Msa0511640-mRNA-1:exon:14893;Parent=Msa0511640-mRNA-1
chr3_4 exon 61240408 61240774 61240408 ID=Msa0511640-mRNA-1:exon:14892;Parent=Msa0511640-mRNA-1
chr3_4 five_prime_UTR 61240553 61240774 61240553 ID=Msa0511640-mRNA-1:five_prime_utr;Parent=Msa0511640-mRNA-1
chr3_4 CDS 61240408 61240552 61240408 ID=Msa0511640-mRNA-1:cds;Parent=Msa0511640-mRNA-1
chr3_4 CDS 61240223 61240299 61240223 ID=Msa0511640-mRNA-1:cds;Parent=Msa0511640-mRNA-1
chr3_4 CDS 61240028 61240105 61240028 ID=Msa0511640-mRNA-1:cds;Parent=Msa0511640-mRNA-1
chr3_4 CDS 61239799 61239872 61239799 ID=Msa0511640-mRNA-1:cds;Parent=Msa0511640-mRNA-1
chr3_4 CDS 61238459 61238690 61238459 ID=Msa0511640-mRNA-1:cds;Parent=Msa0511640-mRNA-1
chr3_4 three_prime_UTR 61238054 61238458 61238054 ID=Msa0511640-mRNA-1:three_prime_utr;Parent=Msa0511640-mRNA-1
Gene Sequence

>Msa0511640

ATGGCTACTGCTGGTGATGTTCTCAGGCAGAGTTTACCGGAAGTAAGTGGTGCTGGTGTTGGAGAGTTGCACGTTCTTGCAGTGGATGATAGTTTTGTTGATCGCAAAGTCATTGAAAGGTTGCTTAAATTCACTTCTTGCAAAGTTACTGTTGTTGAGAGTGGAACTAGAGCTTTACAGTATCTTGGTTTGGATGGTGACACGAGTTCCATTGGTTTTGATGGTGTGAAGGTTAATATGATAATGACAGATTATTCTATGCCTGGGATGACAGGATATGAATTGCTCAAGAAGATTAAGCAGGAATCATCTGTATTTAGAGAAATTCCAGTGGTGGTTATGTCCTCTGAGAACATCTTGACCCGAATTGATAGTTGCTTGGAAGAAGGAGCAGAGGAGTTTCTATTGAAACCTGTCAAGTTGGCAGATGTGAAACGCTTAACAGATTTCATAATGAGAGGTGAAGGAAAGAAAGTCGGAGGAAAAATATCTCAAAAAAGAAGTCGGTCAGATGATTGTATTTCATCTCTATCAACGGCTTGTTCAACATGTGATCTGTCGCCATTGTCACCGTCTGCAATGTCGTCAAAGAAATCCAGACTATAA
Protein sequence

>Msa0511640

MATAGDVLRQSLPEVSGAGVGELHVLAVDDSFVDRKVIERLLKFTSCKVTVVESGTRALQYLGLDGDTSSIGFDGVKVNMIMTDYSMPGMTGYELLKKIKQESSVFREIPVVVMSSENILTRIDSCLEEGAEEFLLKPVKLADVKRLTDFIMRGEGKKVGGKISQKRSRSDDCISSLSTACSTCDLSPLSPSAMSSKKSRL*