AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0373240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0373240 MtrunA17_Chr3g0122491 98.168 382 6 1 14 395 1 381 0.0 775
Msa0373240 MtrunA17_Chr8g0377371 70.461 369 88 3 14 371 1 359 0.0 531
Msa0373240 MtrunA17_Chr3g0132261 62.315 337 112 3 39 373 2 325 4.00e-143 410
Msa0373240 MtrunA17_Chr2g0302201 58.934 319 113 4 39 352 2 307 1.70e-122 358
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0373240 AT1G67340.1 69.516 351 90 6 47 392 38 376 1.10e-172 487
Msa0373240 AT5G50450.1 56.620 355 125 3 36 389 5 331 4.92e-137 395

Find 103 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGTGCTGGTTCTGTTTC+TGG 0.053970 3_1:+72280978 Msa0373240:CDS
TGTCCTTTGCTGAGTGATTT+TGG 0.190426 3_1:+72281008 Msa0373240:CDS
GATATTCGAGGCGGGTTTCC+TGG 0.252033 3_1:+72281089 Msa0373240:CDS
ATGAGGTTATGTTCGAATTC+TGG 0.259191 3_1:+72281119 Msa0373240:CDS
AGGATAACACAAACCTCTTC+TGG 0.297325 3_1:-72280020 None:intergenic
GCTGGTTCTGTTTCTGGTTC+GGG 0.299737 3_1:+72280984 Msa0373240:CDS
TACCATTCCAAGAGTGTAAC+AGG 0.304317 3_1:-72280456 None:intergenic
AGGAAGCGGCGCGTCGTTAA+TGG 0.308133 3_1:+72280611 Msa0373240:CDS
TGCTGGTTCTGTTTCTGGTT+CGG 0.313707 3_1:+72280983 Msa0373240:CDS
TGGTTGTAACGTTCCGGTTC+AGG 0.315466 3_1:+72281028 Msa0373240:CDS
AAAGTACTACTATTGGTAAA+AGG 0.362709 3_1:-72281540 None:intergenic
TGTGCACGCGCTGCATTTCT+CGG 0.363700 3_1:+72280747 Msa0373240:CDS
CGCGCCGTCTTTGTATTCAT+TGG 0.368841 3_1:+72280656 Msa0373240:CDS
GCGCTTGTCAAGCGCTTGAT+TGG 0.371030 3_1:+72281216 Msa0373240:CDS
CTCAAACGCTGTGTTGATGC+TGG 0.395843 3_1:+72280422 Msa0373240:CDS
AACTCATGTTTCCTTGTCTC+AGG 0.404750 3_1:-72281152 None:intergenic
ACGGTAACGGAAGATTGTTT+TGG 0.408205 3_1:+72281393 Msa0373240:three_prime_UTR
ATTGAAGCCTGTTACACTCT+TGG 0.412205 3_1:+72280449 Msa0373240:CDS
TTTGATAGTTGAAGTGTTTG+AGG 0.425616 3_1:-72280040 None:intergenic
CATTGTTTACAAGACGGTTA+CGG 0.429974 3_1:+72280798 Msa0373240:CDS
AACAGATGCAAAAGACTAAA+CGG 0.431674 3_1:+72280308 Msa0373240:intron
TCCGTCATAAACCGGTTCGC+TGG 0.432589 3_1:-72281059 None:intergenic
CAACACAGCGTTTGAGAAAA+CGG 0.444420 3_1:-72280415 None:intergenic
GGTTTCCTGGACCGGGTATG+AGG 0.445338 3_1:+72281102 Msa0373240:CDS
ACATGAGTTTCGAAGATGTT+CGG 0.448702 3_1:+72281166 Msa0373240:CDS
CGAACCACTCCGTCATAAAC+CGG 0.449642 3_1:-72281067 None:intergenic
GAGGAGGAGGAACATGTTGC+CGG 0.452656 3_1:+72281284 Msa0373240:CDS
GCTGACGTGGAGTGTTAACC+CGG 0.456764 3_1:+72280923 Msa0373240:CDS
GCCAGTGGAGAGGTGGTTAG+AGG 0.457892 3_1:+72281265 Msa0373240:CDS
GTTATTGCCGTTAGATAAAA+CGG 0.459886 3_1:-72280886 None:intergenic
GCTGGGTGAGTCTCCTGAAC+CGG 0.464172 3_1:-72281041 None:intergenic
TTGGCAGTGATTCAGTTCAA+TGG 0.465205 3_1:+72280675 Msa0373240:CDS
GGAGAGGTGGTTAGAGGAGG+AGG 0.467062 3_1:+72281271 Msa0373240:CDS
GGTTGTGCTTGATAGCTGAT+AGG 0.470550 3_1:+72281340 Msa0373240:exon
TTTCGAAGATGTTCGGTTTG+TGG 0.474837 3_1:+72281173 Msa0373240:CDS
AGGAGACTCACCCAGCGAAC+CGG 0.480282 3_1:+72281048 Msa0373240:CDS
ATAGAGAGAACTATATCATC+AGG 0.482596 3_1:-72280142 None:intergenic
TCCTCTAACCACCTCTCCAC+TGG 0.483960 3_1:-72281266 None:intergenic
TCGAGGCGGGTTTCCTGGAC+CGG 0.486003 3_1:+72281094 Msa0373240:CDS
GATATAGTTCTCTCTATACT+TGG 0.489280 3_1:+72280148 Msa0373240:CDS
AACACCACCGTCACCTTCAC+CGG 0.490736 3_1:-72281303 None:intergenic
AGATAGATAGATGATAAAGA+AGG 0.491265 3_1:-72279984 None:intergenic
GAAGGGTAAAAGCATAGAGA+GGG 0.495464 3_1:-72279966 None:intergenic
GTGATTCAGTTCAATGGAAG+TGG 0.496025 3_1:+72280681 Msa0373240:CDS
CAAGGCGGAGTGTGCGCCAG+TGG 0.498772 3_1:+72281250 Msa0373240:CDS
GATAGATAGATGATAAAGAA+GGG 0.512654 3_1:-72279983 None:intergenic
TGAGGTGGTTGGTTTGAACC+CGG 0.521097 3_1:-72280942 None:intergenic
ATTATTATGCGAACCAGAAG+AGG 0.526910 3_1:+72280007 Msa0373240:CDS
CCGTCATAAACCGGTTCGCT+GGG 0.529417 3_1:-72281058 None:intergenic
ACAAACAACCGTTGCTGACG+TGG 0.529455 3_1:+72280910 Msa0373240:CDS
GTTCGAATTCTGGTTGTGGA+AGG 0.530016 3_1:+72281129 Msa0373240:CDS
AATGTTAACGGTGACGGTAA+CGG 0.530413 3_1:+72281380 Msa0373240:three_prime_UTR
TGATTGGAAGTTTCGACACA+AGG 0.534148 3_1:+72281232 Msa0373240:CDS
AGAAGGGTAAAAGCATAGAG+AGG 0.538553 3_1:-72279967 None:intergenic
TGTTTGCAAAACAGAGGAAG+CGG 0.541749 3_1:+72280597 Msa0373240:CDS
TTTCAAAATGTTAACGGTGA+CGG 0.543665 3_1:+72281374 Msa0373240:three_prime_UTR
AGAGGAGACCACACTACCAT+TGG 0.543834 3_1:-72281514 None:intergenic
TTATGTTCGAATTCTGGTTG+TGG 0.545212 3_1:+72281125 Msa0373240:CDS
TATTGTCTCATTTATTACTG+TGG 0.546729 3_1:+72281467 Msa0373240:three_prime_UTR
GAGGAACATGTTGCCGGTGA+AGG 0.549146 3_1:+72281290 Msa0373240:CDS
AAAATCTGAAGGAGAATTTG+CGG 0.550795 3_1:-72280185 None:intergenic
ACGGAGTGGTTCGATATTCG+AGG 0.557707 3_1:+72281077 Msa0373240:CDS
CATAACCTCATACCCGGTCC+AGG 0.560065 3_1:-72281107 None:intergenic
GCATTTCTCGGTCACATCGA+CGG 0.562721 3_1:+72280759 Msa0373240:CDS
CGAGGCGGGTTTCCTGGACC+GGG 0.564242 3_1:+72281095 Msa0373240:CDS
GTTAAACAGAACGTTATTGA+AGG 0.570072 3_1:+72280822 Msa0373240:CDS
CGGAGTGTGCGCCAGTGGAG+AGG 0.575589 3_1:+72281255 Msa0373240:CDS
ATCGACGGTTTACGTGAGCT+TGG 0.585324 3_1:+72280774 Msa0373240:CDS
GAGGTGGTTGGTTTGAACCC+GGG 0.585702 3_1:-72280941 None:intergenic
AGTGTGCGCCAGTGGAGAGG+TGG 0.586560 3_1:+72281258 Msa0373240:CDS
AATGCAATTGCAAAGTCCAA+TGG 0.595187 3_1:+72281498 Msa0373240:three_prime_UTR
AAACACGAAGCTGAGGTGGT+TGG 0.595586 3_1:-72280953 None:intergenic
CTTGGTCATTGTTTACAAGA+CGG 0.596462 3_1:+72280792 Msa0373240:CDS
AGCCTGTTACACTCTTGGAA+TGG 0.601007 3_1:+72280454 Msa0373240:CDS
AGAACACTGATAAAATCTGA+AGG 0.607669 3_1:-72280196 None:intergenic
CAAAGACTTACGCGCCGGTG+TGG 0.610989 3_1:+72280719 Msa0373240:CDS
TGCAAAGTCCAATGGTAGTG+TGG 0.612273 3_1:+72281506 Msa0373240:three_prime_UTR
CCCAGCGAACCGGTTTATGA+CGG 0.615877 3_1:+72281058 Msa0373240:CDS
AGTGGTTCGATATTCGAGGC+GGG 0.622558 3_1:+72281081 Msa0373240:CDS
ATTCAGTTCAATGGAAGTGG+AGG 0.623225 3_1:+72280684 Msa0373240:CDS
TGGCATTGGATACTGAAACA+TGG 0.624235 3_1:+72281428 Msa0373240:three_prime_UTR
GCTAAAACACGAAGCTGAGG+TGG 0.627721 3_1:-72280957 None:intergenic
AAGGGTAAAAGCATAGAGAG+GGG 0.628555 3_1:-72279965 None:intergenic
TTAACACTCCACGTCAGCAA+CGG 0.629849 3_1:-72280918 None:intergenic
AGAACGTTATTGAAGGTCGA+CGG 0.630802 3_1:+72280829 Msa0373240:CDS
GTTGTGGAAGGCCTGAGACA+AGG 0.631392 3_1:+72281141 Msa0373240:CDS
AGTGGAGAGGTGGTTAGAGG+AGG 0.634916 3_1:+72281268 Msa0373240:CDS
CAACCAAAATCACTCAGCAA+AGG 0.637304 3_1:-72281011 None:intergenic
CATGTTGCCGGTGAAGGTGA+CGG 0.641096 3_1:+72281296 Msa0373240:CDS
CCAGCTAAAACACGAAGCTG+AGG 0.643105 3_1:-72280960 None:intergenic
TTCGAACATAACCTCATACC+CGG 0.646462 3_1:-72281113 None:intergenic
GCGTGCACATAAAGCCACAC+CGG 0.649753 3_1:-72280733 None:intergenic
AACGACAAAGACTTACGCGC+CGG 0.652915 3_1:+72280714 Msa0373240:CDS
ATCTGAAGGAGAATTTGCGG+TGG 0.661673 3_1:-72280182 None:intergenic
TGGTGTTAACATTGATGAGG+TGG 0.662483 3_1:+72281319 Msa0373240:CDS
GAGTGGTTCGATATTCGAGG+CGG 0.662713 3_1:+72281080 Msa0373240:CDS
CTGACGTGGAGTGTTAACCC+GGG 0.664204 3_1:+72280924 Msa0373240:CDS
ACTATTGGTAAAAGGAACAG+AGG 0.675720 3_1:-72281532 None:intergenic
CGGTGGTGTTAACATTGATG+AGG 0.680359 3_1:+72281316 Msa0373240:CDS
ACTGCCAATGAATACAAAGA+CGG 0.680538 3_1:-72280660 None:intergenic
TTGGAAGTTTCGACACAAGG+CGG 0.683789 3_1:+72281235 Msa0373240:CDS
GTTGCCGGTGAAGGTGACGG+TGG 0.699219 3_1:+72281299 Msa0373240:CDS
CGAACCGGTTTATGACGGAG+TGG 0.704790 3_1:+72281063 Msa0373240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTTTTCAAAATGTTAA+CGG + chr3_1:72281368-72281387 Msa0373240:three_prime_UTR 10.0%
!!! ATTATTTTTCAAAATGTTAA+CGG + chr3_1:72281368-72281387 Msa0373240:three_prime_UTR 10.0%
!!! AAAATTTCTAATTTTTTGAG+TGG - chr3_1:72280483-72280502 None:intergenic 15.0%
!!! AAAATTTCTAATTTTTTGAG+TGG - chr3_1:72280483-72280502 None:intergenic 15.0%
! GATAGATAGATGATAAAGAA+GGG - chr3_1:72279986-72280005 None:intergenic 25.0%
! AGATAGATAGATGATAAAGA+AGG - chr3_1:72279987-72280006 None:intergenic 25.0%
!! TTTTATTGTTTGCAAAACAG+AGG + chr3_1:72280591-72280610 Msa0373240:CDS 25.0%
!!! TGTTTTGGAACTGTATTTTT+TGG + chr3_1:72281408-72281427 Msa0373240:three_prime_UTR 25.0%
! TATTGTCTCATTTATTACTG+TGG + chr3_1:72281467-72281486 Msa0373240:three_prime_UTR 25.0%
!! AAAGTACTACTATTGGTAAA+AGG - chr3_1:72281543-72281562 None:intergenic 25.0%
!!! ACGTAAAAAAGTACTACTAT+TGG - chr3_1:72281550-72281569 None:intergenic 25.0%
! GATAGATAGATGATAAAGAA+GGG - chr3_1:72279986-72280005 None:intergenic 25.0%
! AGATAGATAGATGATAAAGA+AGG - chr3_1:72279987-72280006 None:intergenic 25.0%
!! TTTTATTGTTTGCAAAACAG+AGG + chr3_1:72280591-72280610 Msa0373240:CDS 25.0%
!!! TGTTTTGGAACTGTATTTTT+TGG + chr3_1:72281408-72281427 Msa0373240:three_prime_UTR 25.0%
! TATTGTCTCATTTATTACTG+TGG + chr3_1:72281467-72281486 Msa0373240:three_prime_UTR 25.0%
!! AAAGTACTACTATTGGTAAA+AGG - chr3_1:72281543-72281562 None:intergenic 25.0%
!!! ACGTAAAAAAGTACTACTAT+TGG - chr3_1:72281550-72281569 None:intergenic 25.0%
! TTTGATAGTTGAAGTGTTTG+AGG - chr3_1:72280043-72280062 None:intergenic 30.0%
!!! AAATCATGAGAAGTGTTTTG+TGG - chr3_1:72280109-72280128 None:intergenic 30.0%
ATAGAGAGAACTATATCATC+AGG - chr3_1:72280145-72280164 None:intergenic 30.0%
GATATAGTTCTCTCTATACT+TGG + chr3_1:72280148-72280167 Msa0373240:CDS 30.0%
AAAATCTGAAGGAGAATTTG+CGG - chr3_1:72280188-72280207 None:intergenic 30.0%
AGAACACTGATAAAATCTGA+AGG - chr3_1:72280199-72280218 None:intergenic 30.0%
AACAGATGCAAAAGACTAAA+CGG + chr3_1:72280308-72280327 Msa0373240:intron 30.0%
!! TAATGTTTTGTTCGAAGCTT+TGG - chr3_1:72280360-72280379 None:intergenic 30.0%
TATCAGTCAAACCTAAAAAC+TGG + chr3_1:72280378-72280397 Msa0373240:CDS 30.0%
GTTAAACAGAACGTTATTGA+AGG + chr3_1:72280822-72280841 Msa0373240:CDS 30.0%
GTTATTGCCGTTAGATAAAA+CGG - chr3_1:72280889-72280908 None:intergenic 30.0%
TTTCAAAATGTTAACGGTGA+CGG + chr3_1:72281374-72281393 Msa0373240:three_prime_UTR 30.0%
! TTTGATAGTTGAAGTGTTTG+AGG - chr3_1:72280043-72280062 None:intergenic 30.0%
!!! AAATCATGAGAAGTGTTTTG+TGG - chr3_1:72280109-72280128 None:intergenic 30.0%
ATAGAGAGAACTATATCATC+AGG - chr3_1:72280145-72280164 None:intergenic 30.0%
GATATAGTTCTCTCTATACT+TGG + chr3_1:72280148-72280167 Msa0373240:CDS 30.0%
AAAATCTGAAGGAGAATTTG+CGG - chr3_1:72280188-72280207 None:intergenic 30.0%
AGAACACTGATAAAATCTGA+AGG - chr3_1:72280199-72280218 None:intergenic 30.0%
AACAGATGCAAAAGACTAAA+CGG + chr3_1:72280308-72280327 Msa0373240:intron 30.0%
!! TAATGTTTTGTTCGAAGCTT+TGG - chr3_1:72280360-72280379 None:intergenic 30.0%
TATCAGTCAAACCTAAAAAC+TGG + chr3_1:72280378-72280397 Msa0373240:CDS 30.0%
GTTAAACAGAACGTTATTGA+AGG + chr3_1:72280822-72280841 Msa0373240:CDS 30.0%
GTTATTGCCGTTAGATAAAA+CGG - chr3_1:72280889-72280908 None:intergenic 30.0%
TTTCAAAATGTTAACGGTGA+CGG + chr3_1:72281374-72281393 Msa0373240:three_prime_UTR 30.0%
ATTATTATGCGAACCAGAAG+AGG + chr3_1:72280007-72280026 Msa0373240:CDS 35.0%
ACTGCCAATGAATACAAAGA+CGG - chr3_1:72280663-72280682 None:intergenic 35.0%
CTTGGTCATTGTTTACAAGA+CGG + chr3_1:72280792-72280811 Msa0373240:CDS 35.0%
CATTGTTTACAAGACGGTTA+CGG + chr3_1:72280798-72280817 Msa0373240:CDS 35.0%
!!! TGATTTTGGTTGTAACGTTC+CGG + chr3_1:72281022-72281041 Msa0373240:CDS 35.0%
ATGAGGTTATGTTCGAATTC+TGG + chr3_1:72281119-72281138 Msa0373240:CDS 35.0%
! TTATGTTCGAATTCTGGTTG+TGG + chr3_1:72281125-72281144 Msa0373240:CDS 35.0%
ACATGAGTTTCGAAGATGTT+CGG + chr3_1:72281166-72281185 Msa0373240:CDS 35.0%
!!! GGAACTGTATTTTTTGGCAT+TGG + chr3_1:72281414-72281433 Msa0373240:three_prime_UTR 35.0%
AATGCAATTGCAAAGTCCAA+TGG + chr3_1:72281498-72281517 Msa0373240:three_prime_UTR 35.0%
ACTATTGGTAAAAGGAACAG+AGG - chr3_1:72281535-72281554 None:intergenic 35.0%
ATTATTATGCGAACCAGAAG+AGG + chr3_1:72280007-72280026 Msa0373240:CDS 35.0%
ACTGCCAATGAATACAAAGA+CGG - chr3_1:72280663-72280682 None:intergenic 35.0%
CTTGGTCATTGTTTACAAGA+CGG + chr3_1:72280792-72280811 Msa0373240:CDS 35.0%
CATTGTTTACAAGACGGTTA+CGG + chr3_1:72280798-72280817 Msa0373240:CDS 35.0%
!!! TGATTTTGGTTGTAACGTTC+CGG + chr3_1:72281022-72281041 Msa0373240:CDS 35.0%
ATGAGGTTATGTTCGAATTC+TGG + chr3_1:72281119-72281138 Msa0373240:CDS 35.0%
! TTATGTTCGAATTCTGGTTG+TGG + chr3_1:72281125-72281144 Msa0373240:CDS 35.0%
ACATGAGTTTCGAAGATGTT+CGG + chr3_1:72281166-72281185 Msa0373240:CDS 35.0%
!!! GGAACTGTATTTTTTGGCAT+TGG + chr3_1:72281414-72281433 Msa0373240:three_prime_UTR 35.0%
AATGCAATTGCAAAGTCCAA+TGG + chr3_1:72281498-72281517 Msa0373240:three_prime_UTR 35.0%
ACTATTGGTAAAAGGAACAG+AGG - chr3_1:72281535-72281554 None:intergenic 35.0%
! AAGGGTAAAAGCATAGAGAG+GGG - chr3_1:72279968-72279987 None:intergenic 40.0%
! GAAGGGTAAAAGCATAGAGA+GGG - chr3_1:72279969-72279988 None:intergenic 40.0%
! AGAAGGGTAAAAGCATAGAG+AGG - chr3_1:72279970-72279989 None:intergenic 40.0%
AGGATAACACAAACCTCTTC+TGG - chr3_1:72280023-72280042 None:intergenic 40.0%
CAACACAGCGTTTGAGAAAA+CGG - chr3_1:72280418-72280437 None:intergenic 40.0%
ATTGAAGCCTGTTACACTCT+TGG + chr3_1:72280449-72280468 Msa0373240:CDS 40.0%
! TACCATTCCAAGAGTGTAAC+AGG - chr3_1:72280459-72280478 None:intergenic 40.0%
TGTTTGCAAAACAGAGGAAG+CGG + chr3_1:72280597-72280616 Msa0373240:CDS 40.0%
TTGGCAGTGATTCAGTTCAA+TGG + chr3_1:72280675-72280694 Msa0373240:CDS 40.0%
GTGATTCAGTTCAATGGAAG+TGG + chr3_1:72280681-72280700 Msa0373240:CDS 40.0%
ATTCAGTTCAATGGAAGTGG+AGG + chr3_1:72280684-72280703 Msa0373240:CDS 40.0%
AGAACGTTATTGAAGGTCGA+CGG + chr3_1:72280829-72280848 Msa0373240:CDS 40.0%
! TGTCCTTTGCTGAGTGATTT+TGG + chr3_1:72281008-72281027 Msa0373240:CDS 40.0%
CAACCAAAATCACTCAGCAA+AGG - chr3_1:72281014-72281033 None:intergenic 40.0%
TTCGAACATAACCTCATACC+CGG - chr3_1:72281116-72281135 None:intergenic 40.0%
AACTCATGTTTCCTTGTCTC+AGG - chr3_1:72281155-72281174 None:intergenic 40.0%
! TTTCGAAGATGTTCGGTTTG+TGG + chr3_1:72281173-72281192 Msa0373240:CDS 40.0%
TGATTGGAAGTTTCGACACA+AGG + chr3_1:72281232-72281251 Msa0373240:CDS 40.0%
! TGGTGTTAACATTGATGAGG+TGG + chr3_1:72281319-72281338 Msa0373240:CDS 40.0%
! AATGTTAACGGTGACGGTAA+CGG + chr3_1:72281380-72281399 Msa0373240:three_prime_UTR 40.0%
!!! ACGGTAACGGAAGATTGTTT+TGG + chr3_1:72281393-72281412 Msa0373240:three_prime_UTR 40.0%
! TGGCATTGGATACTGAAACA+TGG + chr3_1:72281428-72281447 Msa0373240:three_prime_UTR 40.0%
! AAGGGTAAAAGCATAGAGAG+GGG - chr3_1:72279968-72279987 None:intergenic 40.0%
! GAAGGGTAAAAGCATAGAGA+GGG - chr3_1:72279969-72279988 None:intergenic 40.0%
! AGAAGGGTAAAAGCATAGAG+AGG - chr3_1:72279970-72279989 None:intergenic 40.0%
AGGATAACACAAACCTCTTC+TGG - chr3_1:72280023-72280042 None:intergenic 40.0%
CAACACAGCGTTTGAGAAAA+CGG - chr3_1:72280418-72280437 None:intergenic 40.0%
ATTGAAGCCTGTTACACTCT+TGG + chr3_1:72280449-72280468 Msa0373240:CDS 40.0%
! TACCATTCCAAGAGTGTAAC+AGG - chr3_1:72280459-72280478 None:intergenic 40.0%
TGTTTGCAAAACAGAGGAAG+CGG + chr3_1:72280597-72280616 Msa0373240:CDS 40.0%
TTGGCAGTGATTCAGTTCAA+TGG + chr3_1:72280675-72280694 Msa0373240:CDS 40.0%
GTGATTCAGTTCAATGGAAG+TGG + chr3_1:72280681-72280700 Msa0373240:CDS 40.0%
ATTCAGTTCAATGGAAGTGG+AGG + chr3_1:72280684-72280703 Msa0373240:CDS 40.0%
AGAACGTTATTGAAGGTCGA+CGG + chr3_1:72280829-72280848 Msa0373240:CDS 40.0%
! TGTCCTTTGCTGAGTGATTT+TGG + chr3_1:72281008-72281027 Msa0373240:CDS 40.0%
CAACCAAAATCACTCAGCAA+AGG - chr3_1:72281014-72281033 None:intergenic 40.0%
TTCGAACATAACCTCATACC+CGG - chr3_1:72281116-72281135 None:intergenic 40.0%
AACTCATGTTTCCTTGTCTC+AGG - chr3_1:72281155-72281174 None:intergenic 40.0%
! TTTCGAAGATGTTCGGTTTG+TGG + chr3_1:72281173-72281192 Msa0373240:CDS 40.0%
TGATTGGAAGTTTCGACACA+AGG + chr3_1:72281232-72281251 Msa0373240:CDS 40.0%
! TGGTGTTAACATTGATGAGG+TGG + chr3_1:72281319-72281338 Msa0373240:CDS 40.0%
! AATGTTAACGGTGACGGTAA+CGG + chr3_1:72281380-72281399 Msa0373240:three_prime_UTR 40.0%
!!! ACGGTAACGGAAGATTGTTT+TGG + chr3_1:72281393-72281412 Msa0373240:three_prime_UTR 40.0%
! TGGCATTGGATACTGAAACA+TGG + chr3_1:72281428-72281447 Msa0373240:three_prime_UTR 40.0%
ATCTGAAGGAGAATTTGCGG+TGG - chr3_1:72280185-72280204 None:intergenic 45.0%
! GCAGAGTCACACCAGTTTTT+AGG - chr3_1:72280392-72280411 None:intergenic 45.0%
AGCCTGTTACACTCTTGGAA+TGG + chr3_1:72280454-72280473 Msa0373240:CDS 45.0%
TTGTCGTGTGTCTGCAGATT+CGG + chr3_1:72280568-72280587 Msa0373240:intron 45.0%
!!! CTCGCTGCCGTTTTATCTAA+CGG + chr3_1:72280879-72280898 Msa0373240:CDS 45.0%
TTAACACTCCACGTCAGCAA+CGG - chr3_1:72280921-72280940 None:intergenic 45.0%
!! TGCTGGTTCTGTTTCTGGTT+CGG + chr3_1:72280983-72281002 Msa0373240:CDS 45.0%
! GTTCGAATTCTGGTTGTGGA+AGG + chr3_1:72281129-72281148 Msa0373240:CDS 45.0%
TTGGAAGTTTCGACACAAGG+CGG + chr3_1:72281235-72281254 Msa0373240:CDS 45.0%
! CGGTGGTGTTAACATTGATG+AGG + chr3_1:72281316-72281335 Msa0373240:CDS 45.0%
! GGTTGTGCTTGATAGCTGAT+AGG + chr3_1:72281340-72281359 Msa0373240:exon 45.0%
! TGCAAAGTCCAATGGTAGTG+TGG + chr3_1:72281506-72281525 Msa0373240:three_prime_UTR 45.0%
ATCTGAAGGAGAATTTGCGG+TGG - chr3_1:72280185-72280204 None:intergenic 45.0%
! GCAGAGTCACACCAGTTTTT+AGG - chr3_1:72280392-72280411 None:intergenic 45.0%
AGCCTGTTACACTCTTGGAA+TGG + chr3_1:72280454-72280473 Msa0373240:CDS 45.0%
TTGTCGTGTGTCTGCAGATT+CGG + chr3_1:72280568-72280587 Msa0373240:intron 45.0%
!!! CTCGCTGCCGTTTTATCTAA+CGG + chr3_1:72280879-72280898 Msa0373240:CDS 45.0%
TTAACACTCCACGTCAGCAA+CGG - chr3_1:72280921-72280940 None:intergenic 45.0%
!! TGCTGGTTCTGTTTCTGGTT+CGG + chr3_1:72280983-72281002 Msa0373240:CDS 45.0%
! GTTCGAATTCTGGTTGTGGA+AGG + chr3_1:72281129-72281148 Msa0373240:CDS 45.0%
TTGGAAGTTTCGACACAAGG+CGG + chr3_1:72281235-72281254 Msa0373240:CDS 45.0%
! CGGTGGTGTTAACATTGATG+AGG + chr3_1:72281316-72281335 Msa0373240:CDS 45.0%
! GGTTGTGCTTGATAGCTGAT+AGG + chr3_1:72281340-72281359 Msa0373240:exon 45.0%
! TGCAAAGTCCAATGGTAGTG+TGG + chr3_1:72281506-72281525 Msa0373240:three_prime_UTR 45.0%
! CTCAAACGCTGTGTTGATGC+TGG + chr3_1:72280422-72280441 Msa0373240:CDS 50.0%
CGCGCCGTCTTTGTATTCAT+TGG + chr3_1:72280656-72280675 Msa0373240:CDS 50.0%
AACGACAAAGACTTACGCGC+CGG + chr3_1:72280714-72280733 Msa0373240:CDS 50.0%
GCATTTCTCGGTCACATCGA+CGG + chr3_1:72280759-72280778 Msa0373240:CDS 50.0%
ATCGACGGTTTACGTGAGCT+TGG + chr3_1:72280774-72280793 Msa0373240:CDS 50.0%
ACAAACAACCGTTGCTGACG+TGG + chr3_1:72280910-72280929 Msa0373240:CDS 50.0%
!! TGAGGTGGTTGGTTTGAACC+CGG - chr3_1:72280945-72280964 None:intergenic 50.0%
AAACACGAAGCTGAGGTGGT+TGG - chr3_1:72280956-72280975 None:intergenic 50.0%
GCTAAAACACGAAGCTGAGG+TGG - chr3_1:72280960-72280979 None:intergenic 50.0%
CCAGCTAAAACACGAAGCTG+AGG - chr3_1:72280963-72280982 None:intergenic 50.0%
!!! CCTCAGCTTCGTGTTTTAGC+TGG + chr3_1:72280960-72280979 Msa0373240:CDS 50.0%
!!! CTTCGTGTTTTAGCTGGTGC+TGG + chr3_1:72280966-72280985 Msa0373240:CDS 50.0%
!! GCTGGTTCTGTTTCTGGTTC+GGG + chr3_1:72280984-72281003 Msa0373240:CDS 50.0%
TGGTTGTAACGTTCCGGTTC+AGG + chr3_1:72281028-72281047 Msa0373240:CDS 50.0%
CGAACCACTCCGTCATAAAC+CGG - chr3_1:72281070-72281089 None:intergenic 50.0%
ACGGAGTGGTTCGATATTCG+AGG + chr3_1:72281077-72281096 Msa0373240:CDS 50.0%
GAGTGGTTCGATATTCGAGG+CGG + chr3_1:72281080-72281099 Msa0373240:CDS 50.0%
AGTGGTTCGATATTCGAGGC+GGG + chr3_1:72281081-72281100 Msa0373240:CDS 50.0%
AGAGGAGACCACACTACCAT+TGG - chr3_1:72281517-72281536 None:intergenic 50.0%
! CTCAAACGCTGTGTTGATGC+TGG + chr3_1:72280422-72280441 Msa0373240:CDS 50.0%
CGCGCCGTCTTTGTATTCAT+TGG + chr3_1:72280656-72280675 Msa0373240:CDS 50.0%
AACGACAAAGACTTACGCGC+CGG + chr3_1:72280714-72280733 Msa0373240:CDS 50.0%
GCATTTCTCGGTCACATCGA+CGG + chr3_1:72280759-72280778 Msa0373240:CDS 50.0%
ATCGACGGTTTACGTGAGCT+TGG + chr3_1:72280774-72280793 Msa0373240:CDS 50.0%
ACAAACAACCGTTGCTGACG+TGG + chr3_1:72280910-72280929 Msa0373240:CDS 50.0%
!! TGAGGTGGTTGGTTTGAACC+CGG - chr3_1:72280945-72280964 None:intergenic 50.0%
AAACACGAAGCTGAGGTGGT+TGG - chr3_1:72280956-72280975 None:intergenic 50.0%
GCTAAAACACGAAGCTGAGG+TGG - chr3_1:72280960-72280979 None:intergenic 50.0%
CCAGCTAAAACACGAAGCTG+AGG - chr3_1:72280963-72280982 None:intergenic 50.0%
!!! CCTCAGCTTCGTGTTTTAGC+TGG + chr3_1:72280960-72280979 Msa0373240:CDS 50.0%
!!! CTTCGTGTTTTAGCTGGTGC+TGG + chr3_1:72280966-72280985 Msa0373240:CDS 50.0%
!! GCTGGTTCTGTTTCTGGTTC+GGG + chr3_1:72280984-72281003 Msa0373240:CDS 50.0%
TGGTTGTAACGTTCCGGTTC+AGG + chr3_1:72281028-72281047 Msa0373240:CDS 50.0%
CGAACCACTCCGTCATAAAC+CGG - chr3_1:72281070-72281089 None:intergenic 50.0%
ACGGAGTGGTTCGATATTCG+AGG + chr3_1:72281077-72281096 Msa0373240:CDS 50.0%
GAGTGGTTCGATATTCGAGG+CGG + chr3_1:72281080-72281099 Msa0373240:CDS 50.0%
AGTGGTTCGATATTCGAGGC+GGG + chr3_1:72281081-72281100 Msa0373240:CDS 50.0%
AGAGGAGACCACACTACCAT+TGG - chr3_1:72281517-72281536 None:intergenic 50.0%
GCGTGCACATAAAGCCACAC+CGG - chr3_1:72280736-72280755 None:intergenic 55.0%
TGTGCACGCGCTGCATTTCT+CGG + chr3_1:72280747-72280766 Msa0373240:CDS 55.0%
! GCTGACGTGGAGTGTTAACC+CGG + chr3_1:72280923-72280942 Msa0373240:CDS 55.0%
! CTGACGTGGAGTGTTAACCC+GGG + chr3_1:72280924-72280943 Msa0373240:CDS 55.0%
!! GAGGTGGTTGGTTTGAACCC+GGG - chr3_1:72280944-72280963 None:intergenic 55.0%
!! GCTGGTGCTGGTTCTGTTTC+TGG + chr3_1:72280978-72280997 Msa0373240:CDS 55.0%
!! CCGTCATAAACCGGTTCGCT+GGG - chr3_1:72281061-72281080 None:intergenic 55.0%
! CCCAGCGAACCGGTTTATGA+CGG + chr3_1:72281058-72281077 Msa0373240:CDS 55.0%
!! TCCGTCATAAACCGGTTCGC+TGG - chr3_1:72281062-72281081 None:intergenic 55.0%
! CGAACCGGTTTATGACGGAG+TGG + chr3_1:72281063-72281082 Msa0373240:CDS 55.0%
! GATATTCGAGGCGGGTTTCC+TGG + chr3_1:72281089-72281108 Msa0373240:CDS 55.0%
CATAACCTCATACCCGGTCC+AGG - chr3_1:72281110-72281129 None:intergenic 55.0%
GTTGTGGAAGGCCTGAGACA+AGG + chr3_1:72281141-72281160 Msa0373240:CDS 55.0%
GCGCTTGTCAAGCGCTTGAT+TGG + chr3_1:72281216-72281235 Msa0373240:CDS 55.0%
TCCTCTAACCACCTCTCCAC+TGG - chr3_1:72281269-72281288 None:intergenic 55.0%
AGTGGAGAGGTGGTTAGAGG+AGG + chr3_1:72281268-72281287 Msa0373240:CDS 55.0%
GAGGAGGAGGAACATGTTGC+CGG + chr3_1:72281284-72281303 Msa0373240:CDS 55.0%
GAGGAACATGTTGCCGGTGA+AGG + chr3_1:72281290-72281309 Msa0373240:CDS 55.0%
CATGTTGCCGGTGAAGGTGA+CGG + chr3_1:72281296-72281315 Msa0373240:CDS 55.0%
AACACCACCGTCACCTTCAC+CGG - chr3_1:72281306-72281325 None:intergenic 55.0%
GCGTGCACATAAAGCCACAC+CGG - chr3_1:72280736-72280755 None:intergenic 55.0%
TGTGCACGCGCTGCATTTCT+CGG + chr3_1:72280747-72280766 Msa0373240:CDS 55.0%
! GCTGACGTGGAGTGTTAACC+CGG + chr3_1:72280923-72280942 Msa0373240:CDS 55.0%
! CTGACGTGGAGTGTTAACCC+GGG + chr3_1:72280924-72280943 Msa0373240:CDS 55.0%
!! GAGGTGGTTGGTTTGAACCC+GGG - chr3_1:72280944-72280963 None:intergenic 55.0%
!! GCTGGTGCTGGTTCTGTTTC+TGG + chr3_1:72280978-72280997 Msa0373240:CDS 55.0%
!! CCGTCATAAACCGGTTCGCT+GGG - chr3_1:72281061-72281080 None:intergenic 55.0%
! CCCAGCGAACCGGTTTATGA+CGG + chr3_1:72281058-72281077 Msa0373240:CDS 55.0%
!! TCCGTCATAAACCGGTTCGC+TGG - chr3_1:72281062-72281081 None:intergenic 55.0%
! CGAACCGGTTTATGACGGAG+TGG + chr3_1:72281063-72281082 Msa0373240:CDS 55.0%
! GATATTCGAGGCGGGTTTCC+TGG + chr3_1:72281089-72281108 Msa0373240:CDS 55.0%
CATAACCTCATACCCGGTCC+AGG - chr3_1:72281110-72281129 None:intergenic 55.0%
GTTGTGGAAGGCCTGAGACA+AGG + chr3_1:72281141-72281160 Msa0373240:CDS 55.0%
GCGCTTGTCAAGCGCTTGAT+TGG + chr3_1:72281216-72281235 Msa0373240:CDS 55.0%
TCCTCTAACCACCTCTCCAC+TGG - chr3_1:72281269-72281288 None:intergenic 55.0%
AGTGGAGAGGTGGTTAGAGG+AGG + chr3_1:72281268-72281287 Msa0373240:CDS 55.0%
GAGGAGGAGGAACATGTTGC+CGG + chr3_1:72281284-72281303 Msa0373240:CDS 55.0%
GAGGAACATGTTGCCGGTGA+AGG + chr3_1:72281290-72281309 Msa0373240:CDS 55.0%
CATGTTGCCGGTGAAGGTGA+CGG + chr3_1:72281296-72281315 Msa0373240:CDS 55.0%
AACACCACCGTCACCTTCAC+CGG - chr3_1:72281306-72281325 None:intergenic 55.0%
AGGAAGCGGCGCGTCGTTAA+TGG + chr3_1:72280611-72280630 Msa0373240:CDS 60.0%
! CAAAGACTTACGCGCCGGTG+TGG + chr3_1:72280719-72280738 Msa0373240:CDS 60.0%
GCTGGGTGAGTCTCCTGAAC+CGG - chr3_1:72281044-72281063 None:intergenic 60.0%
AGGAGACTCACCCAGCGAAC+CGG + chr3_1:72281048-72281067 Msa0373240:CDS 60.0%
GGTTTCCTGGACCGGGTATG+AGG + chr3_1:72281102-72281121 Msa0373240:CDS 60.0%
GCCAGTGGAGAGGTGGTTAG+AGG + chr3_1:72281265-72281284 Msa0373240:CDS 60.0%
GGAGAGGTGGTTAGAGGAGG+AGG + chr3_1:72281271-72281290 Msa0373240:CDS 60.0%
AGGAAGCGGCGCGTCGTTAA+TGG + chr3_1:72280611-72280630 Msa0373240:CDS 60.0%
! CAAAGACTTACGCGCCGGTG+TGG + chr3_1:72280719-72280738 Msa0373240:CDS 60.0%
GCTGGGTGAGTCTCCTGAAC+CGG - chr3_1:72281044-72281063 None:intergenic 60.0%
AGGAGACTCACCCAGCGAAC+CGG + chr3_1:72281048-72281067 Msa0373240:CDS 60.0%
GGTTTCCTGGACCGGGTATG+AGG + chr3_1:72281102-72281121 Msa0373240:CDS 60.0%
GCCAGTGGAGAGGTGGTTAG+AGG + chr3_1:72281265-72281284 Msa0373240:CDS 60.0%
GGAGAGGTGGTTAGAGGAGG+AGG + chr3_1:72281271-72281290 Msa0373240:CDS 60.0%
! TCGAGGCGGGTTTCCTGGAC+CGG + chr3_1:72281094-72281113 Msa0373240:CDS 65.0%
AGTGTGCGCCAGTGGAGAGG+TGG + chr3_1:72281258-72281277 Msa0373240:CDS 65.0%
! GTTGCCGGTGAAGGTGACGG+TGG + chr3_1:72281299-72281318 Msa0373240:CDS 65.0%
! TCGAGGCGGGTTTCCTGGAC+CGG + chr3_1:72281094-72281113 Msa0373240:CDS 65.0%
AGTGTGCGCCAGTGGAGAGG+TGG + chr3_1:72281258-72281277 Msa0373240:CDS 65.0%
! GTTGCCGGTGAAGGTGACGG+TGG + chr3_1:72281299-72281318 Msa0373240:CDS 65.0%
! CGAGGCGGGTTTCCTGGACC+GGG + chr3_1:72281095-72281114 Msa0373240:CDS 70.0%
CAAGGCGGAGTGTGCGCCAG+TGG + chr3_1:72281250-72281269 Msa0373240:CDS 70.0%
CGGAGTGTGCGCCAGTGGAG+AGG + chr3_1:72281255-72281274 Msa0373240:CDS 70.0%
! CGAGGCGGGTTTCCTGGACC+GGG + chr3_1:72281095-72281114 Msa0373240:CDS 70.0%
CAAGGCGGAGTGTGCGCCAG+TGG + chr3_1:72281250-72281269 Msa0373240:CDS 70.0%
CGGAGTGTGCGCCAGTGGAG+AGG + chr3_1:72281255-72281274 Msa0373240:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 72279961 72281623 72279961 ID=Msa0373240;Name=Msa0373240
chr3_1 mRNA 72279961 72281623 72279961 ID=Msa0373240-mRNA-1;Parent=Msa0373240;Name=Msa0373240-mRNA-1;_AED=0.04;_eAED=0.04;_QI=13|1|1|1|1|1|3|265|395
chr3_1 exon 72279961 72280224 72279961 ID=Msa0373240-mRNA-1:exon:16022;Parent=Msa0373240-mRNA-1
chr3_1 exon 72280313 72280475 72280313 ID=Msa0373240-mRNA-1:exon:16023;Parent=Msa0373240-mRNA-1
chr3_1 exon 72280585 72281623 72280585 ID=Msa0373240-mRNA-1:exon:16024;Parent=Msa0373240-mRNA-1
chr3_1 five_prime_UTR 72279961 72279973 72279961 ID=Msa0373240-mRNA-1:five_prime_utr;Parent=Msa0373240-mRNA-1
chr3_1 CDS 72279974 72280224 72279974 ID=Msa0373240-mRNA-1:cds;Parent=Msa0373240-mRNA-1
chr3_1 CDS 72280313 72280475 72280313 ID=Msa0373240-mRNA-1:cds;Parent=Msa0373240-mRNA-1
chr3_1 CDS 72280585 72281358 72280585 ID=Msa0373240-mRNA-1:cds;Parent=Msa0373240-mRNA-1
chr3_1 three_prime_UTR 72281359 72281623 72281359 ID=Msa0373240-mRNA-1:three_prime_utr;Parent=Msa0373240-mRNA-1
Gene Sequence

>Msa0373240

ATGCTTTTACCCTTCTTTATCATCTATCTATCTATTATTATGCGAACCAGAAGAGGTTTGTGTTATCCTCAAACACTTCAACTATCAAACATGTTTTCAGACACAAACAATAACAAAAGACACAAGAGAATGCCACAAAACACTTCTCATGATTTCTTTGAACTTTTGCCTGATGATATAGTTCTCTCTATACTTGGAAAACTCAGCTCCACCGCAAATTCTCCTTCAGATTTTATCAGTGTTCTAATCACATGCAAAAGACTAAACGGCTTAAGTCTTAACTCTGTTGTTTTGTCCAAAGCTTCGAACAAAACATTATCAGTCAAACCTAAAAACTGGTGTGACTCTGCTCACCGTTTTCTCAAACGCTGTGTTGATGCTGGAAACATTGAAGCCTGTTACACTCTTGGAATGATTCGGTTTTATTGTTTGCAAAACAGAGGAAGCGGCGCGTCGTTAATGGCAAAAGCAGCTATGAAATCTCACGCGCCGTCTTTGTATTCATTGGCAGTGATTCAGTTCAATGGAAGTGGAGGAACAAAAAACGACAAAGACTTACGCGCCGGTGTGGCTTTATGTGCACGCGCTGCATTTCTCGGTCACATCGACGGTTTACGTGAGCTTGGTCATTGTTTACAAGACGGTTACGGCGTTAAACAGAACGTTATTGAAGGTCGACGGTTTCTAGTTCAAGCTAATGCTCGTGAACTCGCTGCCGTTTTATCTAACGGCAATAACAACAAACAACCGTTGCTGACGTGGAGTGTTAACCCGGGTTCAAACCAACCACCTCAGCTTCGTGTTTTAGCTGGTGCTGGTTCTGTTTCTGGTTCGGGATGTCCTTTGCTGAGTGATTTTGGTTGTAACGTTCCGGTTCAGGAGACTCACCCAGCGAACCGGTTTATGACGGAGTGGTTCGATATTCGAGGCGGGTTTCCTGGACCGGGTATGAGGTTATGTTCGAATTCTGGTTGTGGAAGGCCTGAGACAAGGAAACATGAGTTTCGAAGATGTTCGGTTTGTGGTGTTGTGAATTATTGTTCACGCGCTTGTCAAGCGCTTGATTGGAAGTTTCGACACAAGGCGGAGTGTGCGCCAGTGGAGAGGTGGTTAGAGGAGGAGGAACATGTTGCCGGTGAAGGTGACGGTGGTGTTAACATTGATGAGGTGGTTGTGCTTGATAGCTGA
Protein sequence

>Msa0373240

MLLPFFIIYLSIIMRTRRGLCYPQTLQLSNMFSDTNNNKRHKRMPQNTSHDFFELLPDDIVLSILGKLSSTANSPSDFISVLITCKRLNGLSLNSVVLSKASNKTLSVKPKNWCDSAHRFLKRCVDAGNIEACYTLGMIRFYCLQNRGSGASLMAKAAMKSHAPSLYSLAVIQFNGSGGTKNDKDLRAGVALCARAAFLGHIDGLRELGHCLQDGYGVKQNVIEGRRFLVQANARELAAVLSNGNNNKQPLLTWSVNPGSNQPPQLRVLAGAGSVSGSGCPLLSDFGCNVPVQETHPANRFMTEWFDIRGGFPGPGMRLCSNSGCGRPETRKHEFRRCSVCGVVNYCSRACQALDWKFRHKAECAPVERWLEEEEHVAGEGDGGVNIDEVVVLDS*