AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0421830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0421830 MtrunA17_Chr3g0122491 96.859 382 11 1 1 382 1 381 0.0 763
Msa0421830 MtrunA17_Chr8g0377371 71.003 369 86 4 1 358 1 359 0.0 526
Msa0421830 MtrunA17_Chr3g0132261 61.128 337 116 2 26 360 2 325 3.19e-142 408
Msa0421830 MtrunA17_Chr2g0302201 58.307 319 115 4 26 339 2 307 2.17e-121 355
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0421830 AT1G67340.1 70.262 343 87 5 34 375 38 366 8.24e-173 487
Msa0421830 AT5G50450.1 56.056 355 127 3 23 376 5 331 3.07e-135 390

Find 105 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGTTTCTGGTTCTGTTTC+TGG 0.024919 3_2:+70874277 Msa0421830:CDS
TGTCCTTTGCTGAGTGATTT+TGG 0.190426 3_2:+70874307 Msa0421830:CDS
GATATTCGAGGCGGGTTTCC+TGG 0.252033 3_2:+70874388 Msa0421830:CDS
ATGAGGTTATGTTCGAATTC+TGG 0.259191 3_2:+70874418 Msa0421830:CDS
TTCTGGTTCTGTTTCTGGTT+CGG 0.289711 3_2:+70874282 Msa0421830:CDS
TCTGGTTCTGTTTCTGGTTC+GGG 0.290808 3_2:+70874283 Msa0421830:CDS
AGGATAACACAAACCTCTTC+TGG 0.297325 3_2:-70873315 None:intergenic
TACCATTCCAAGAGTGTAAC+AGG 0.304317 3_2:-70873755 None:intergenic
TGGTTGTAACGTTCCGGTTC+AGG 0.315466 3_2:+70874327 Msa0421830:CDS
AAAGTACTACTATTGGTAAA+AGG 0.362709 3_2:-70874839 None:intergenic
TGTGCACGCGCTGCATTTCT+CGG 0.363700 3_2:+70874046 Msa0421830:CDS
CGCGCCGTCTTTGTATTCAT+TGG 0.368841 3_2:+70873955 Msa0421830:CDS
GCGCTTGTCAAGCGCTTGAT+TGG 0.371030 3_2:+70874515 Msa0421830:CDS
CTCAAACGCTGTGTTGATGC+TGG 0.395843 3_2:+70873721 Msa0421830:CDS
AACTCATGTTTCCTTGTCTC+AGG 0.404750 3_2:-70874451 None:intergenic
ACGGTAACGGAAGATTGTTT+TGG 0.408205 3_2:+70874692 Msa0421830:three_prime_UTR
ATTGAAGCCTGTTACACTCT+TGG 0.412205 3_2:+70873748 Msa0421830:CDS
AGGAAGCGGCGCGTCGTTGA+TGG 0.424760 3_2:+70873910 Msa0421830:CDS
TTTGATAGTTGAAGTGTTTG+AGG 0.425616 3_2:-70873335 None:intergenic
CATTGTTTACAAGACGGTTA+CGG 0.429974 3_2:+70874097 Msa0421830:CDS
AACAGATGCAAAAGACTAAA+CGG 0.431674 3_2:+70873607 Msa0421830:intron
CAACACAGCGTTTGAGAAAA+CGG 0.444420 3_2:-70873714 None:intergenic
GGTTTCCTGGACCGGGTATG+AGG 0.445338 3_2:+70874401 Msa0421830:CDS
ACATGAGTTTCGAAGATGTT+CGG 0.448702 3_2:+70874465 Msa0421830:CDS
GAGGAGGAGGAACATGTTGC+CGG 0.452656 3_2:+70874583 Msa0421830:CDS
GCTGACGTGGAGTGTTAACC+CGG 0.456764 3_2:+70874222 Msa0421830:CDS
GCCAGTGGAGAGGTGGTTAG+AGG 0.457892 3_2:+70874564 Msa0421830:CDS
GTTATTGCCGTTAGATAAAA+CGG 0.459886 3_2:-70874185 None:intergenic
CCCATCATAAACCGGTTCGC+TGG 0.462026 3_2:-70874358 None:intergenic
GCTGGGTGAGTCTCCTGAAC+CGG 0.464172 3_2:-70874340 None:intergenic
TTGGCAGTGATTCAGTTCAA+TGG 0.465205 3_2:+70873974 Msa0421830:CDS
GGAGAGGTGGTTAGAGGAGG+AGG 0.467062 3_2:+70874570 Msa0421830:CDS
GGTTGTGCTTGATAGCTGAT+AGG 0.470550 3_2:+70874639 Msa0421830:exon
CCCAGCGAACCGGTTTATGA+TGG 0.473236 3_2:+70874357 Msa0421830:CDS
TTTCGAAGATGTTCGGTTTG+TGG 0.474837 3_2:+70874472 Msa0421830:CDS
AGGAGACTCACCCAGCGAAC+CGG 0.480282 3_2:+70874347 Msa0421830:CDS
ATAGAGAGAACTATATCATC+AGG 0.482596 3_2:-70873437 None:intergenic
TCCTCTAACCACCTCTCCAC+TGG 0.483960 3_2:-70874565 None:intergenic
TCGAGGCGGGTTTCCTGGAC+CGG 0.486003 3_2:+70874393 Msa0421830:CDS
GATATAGTTCTCTCTATACT+TGG 0.489280 3_2:+70873443 Msa0421830:CDS
AACACCACCGTCACCTTCAC+CGG 0.490736 3_2:-70874602 None:intergenic
TAATAGATAGATGATAAAGA+AGG 0.492994 3_2:-70873283 None:intergenic
GAAGGGTAAAAGCATAGAGA+GGG 0.495464 3_2:-70873265 None:intergenic
GTGATTCAGTTCAATGGAAG+TGG 0.496025 3_2:+70873980 Msa0421830:CDS
CAAGGCGGAGTGTGCGCCAG+TGG 0.498772 3_2:+70874549 Msa0421830:CDS
CCAGCGAACCGGTTTATGAT+GGG 0.509332 3_2:+70874358 Msa0421830:CDS
AATAGATAGATGATAAAGAA+GGG 0.516305 3_2:-70873282 None:intergenic
CGAACCACCCCATCATAAAC+CGG 0.517520 3_2:-70874366 None:intergenic
TGAGGTGGTTGGTTTGAACC+CGG 0.521097 3_2:-70874241 None:intergenic
ATTATTATGCGAACCAGAAG+AGG 0.526910 3_2:+70873302 Msa0421830:exon
ACAAACAACCGTTGCTGACG+TGG 0.529455 3_2:+70874209 Msa0421830:CDS
GTTCGAATTCTGGTTGTGGA+AGG 0.530016 3_2:+70874428 Msa0421830:CDS
AATGTTAACGGTGACGGTAA+CGG 0.530413 3_2:+70874679 Msa0421830:three_prime_UTR
TGATTGGAAGTTTCGACACA+AGG 0.534148 3_2:+70874531 Msa0421830:CDS
CCATCATAAACCGGTTCGCT+GGG 0.538212 3_2:-70874357 None:intergenic
AGAAGGGTAAAAGCATAGAG+AGG 0.538553 3_2:-70873266 None:intergenic
TTTCAAAATGTTAACGGTGA+CGG 0.543665 3_2:+70874673 Msa0421830:three_prime_UTR
AGAGGAGACCACACTACCAT+TGG 0.543834 3_2:-70874813 None:intergenic
TTATGTTCGAATTCTGGTTG+TGG 0.545212 3_2:+70874424 Msa0421830:CDS
TATTGTCTCATTTATTACTG+TGG 0.546729 3_2:+70874766 Msa0421830:three_prime_UTR
GAGGAACATGTTGCCGGTGA+AGG 0.549146 3_2:+70874589 Msa0421830:CDS
AAAATCTGAAGGAGAATTTG+CGG 0.550795 3_2:-70873480 None:intergenic
CATAACCTCATACCCGGTCC+AGG 0.560065 3_2:-70874406 None:intergenic
GCATTTCTCGGTCACATCGA+CGG 0.562721 3_2:+70874058 Msa0421830:CDS
TGTTTGCAAAACAGAGGAAG+CGG 0.562893 3_2:+70873896 Msa0421830:CDS
CGAGGCGGGTTTCCTGGACC+GGG 0.564242 3_2:+70874394 Msa0421830:CDS
ATGGGGTGGTTCGATATTCG+AGG 0.569412 3_2:+70874376 Msa0421830:CDS
GTTAAACAGAACGTTATTGA+AGG 0.570072 3_2:+70874121 Msa0421830:CDS
CGGAGTGTGCGCCAGTGGAG+AGG 0.575589 3_2:+70874554 Msa0421830:CDS
ATCGACGGTTTACGTGAGCT+TGG 0.585324 3_2:+70874073 Msa0421830:CDS
GAGGTGGTTGGTTTGAACCC+GGG 0.585702 3_2:-70874240 None:intergenic
AGTGTGCGCCAGTGGAGAGG+TGG 0.586560 3_2:+70874557 Msa0421830:CDS
AATGCAATTGCAAAGTCCAA+TGG 0.595187 3_2:+70874797 Msa0421830:three_prime_UTR
AAACACGAAGCTGAGGTGGT+TGG 0.595586 3_2:-70874252 None:intergenic
CTTGGTCATTGTTTACAAGA+CGG 0.596462 3_2:+70874091 Msa0421830:CDS
AGCCTGTTACACTCTTGGAA+TGG 0.601007 3_2:+70873753 Msa0421830:CDS
AGAACACTGATAAAATCTGA+AGG 0.607669 3_2:-70873491 None:intergenic
CAAAGACTTACGCGCCGGTG+TGG 0.610989 3_2:+70874018 Msa0421830:CDS
TGCAAAGTCCAATGGTAGTG+TGG 0.612273 3_2:+70874805 Msa0421830:three_prime_UTR
GCTAAAACACGAAGCTGAGG+TGG 0.620900 3_2:-70874256 None:intergenic
ATTCAGTTCAATGGAAGTGG+AGG 0.623225 3_2:+70873983 Msa0421830:CDS
TGGCATTGGATACTGAAACA+TGG 0.624235 3_2:+70874727 Msa0421830:three_prime_UTR
AAGGGTAAAAGCATAGAGAG+GGG 0.628555 3_2:-70873264 None:intergenic
CGAACCGGTTTATGATGGGG+TGG 0.628937 3_2:+70874362 Msa0421830:CDS
TTAACACTCCACGTCAGCAA+CGG 0.629849 3_2:-70874217 None:intergenic
AGAACGTTATTGAAGGTCGA+CGG 0.630802 3_2:+70874128 Msa0421830:CDS
GTTGTGGAAGGCCTGAGACA+AGG 0.631392 3_2:+70874440 Msa0421830:CDS
AGTGGAGAGGTGGTTAGAGG+AGG 0.634916 3_2:+70874567 Msa0421830:CDS
CAACCAAAATCACTCAGCAA+AGG 0.637304 3_2:-70874310 None:intergenic
CATGTTGCCGGTGAAGGTGA+CGG 0.641096 3_2:+70874595 Msa0421830:CDS
GGTGGTTCGATATTCGAGGC+GGG 0.643025 3_2:+70874380 Msa0421830:CDS
ACAGCTAAAACACGAAGCTG+AGG 0.646405 3_2:-70874259 None:intergenic
TTCGAACATAACCTCATACC+CGG 0.646462 3_2:-70874412 None:intergenic
GCGTGCACATAAAGCCACAC+CGG 0.649753 3_2:-70874032 None:intergenic
AACGACAAAGACTTACGCGC+CGG 0.652915 3_2:+70874013 Msa0421830:CDS
CAGCGAACCGGTTTATGATG+GGG 0.653162 3_2:+70874359 Msa0421830:CDS
ATCTGAAGGAGAATTTGCGG+TGG 0.661673 3_2:-70873477 None:intergenic
TGGTGTTAACATTGATGAGG+TGG 0.662483 3_2:+70874618 Msa0421830:CDS
CTGACGTGGAGTGTTAACCC+GGG 0.664204 3_2:+70874223 Msa0421830:CDS
ACTATTGGTAAAAGGAACAG+AGG 0.675720 3_2:-70874831 None:intergenic
GGGTGGTTCGATATTCGAGG+CGG 0.680012 3_2:+70874379 Msa0421830:CDS
CGGTGGTGTTAACATTGATG+AGG 0.680359 3_2:+70874615 Msa0421830:CDS
ACTGCCAATGAATACAAAGA+CGG 0.680538 3_2:-70873959 None:intergenic
TTGGAAGTTTCGACACAAGG+CGG 0.683789 3_2:+70874534 Msa0421830:CDS
GTTGCCGGTGAAGGTGACGG+TGG 0.699219 3_2:+70874598 Msa0421830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTTTTCAAAATGTTAA+CGG + chr3_2:70874667-70874686 Msa0421830:three_prime_UTR 10.0%
!!! ATTATTTTTCAAAATGTTAA+CGG + chr3_2:70874667-70874686 Msa0421830:three_prime_UTR 10.0%
!!! AAAATTTCTAATTTTTTGAG+TGG - chr3_2:70873782-70873801 None:intergenic 15.0%
!!! AAAATTTCTAATTTTTTGAG+TGG - chr3_2:70873782-70873801 None:intergenic 15.0%
!! AATAGATAGATGATAAAGAA+GGG - chr3_2:70873285-70873304 None:intergenic 20.0%
!! TAATAGATAGATGATAAAGA+AGG - chr3_2:70873286-70873305 None:intergenic 20.0%
!! AATAGATAGATGATAAAGAA+GGG - chr3_2:70873285-70873304 None:intergenic 20.0%
!! TAATAGATAGATGATAAAGA+AGG - chr3_2:70873286-70873305 None:intergenic 20.0%
!!! TGTTTTGGAACTGTATTTTT+TGG + chr3_2:70874707-70874726 Msa0421830:three_prime_UTR 25.0%
! TATTGTCTCATTTATTACTG+TGG + chr3_2:70874766-70874785 Msa0421830:three_prime_UTR 25.0%
!! AAAGTACTACTATTGGTAAA+AGG - chr3_2:70874842-70874861 None:intergenic 25.0%
!!! ACGTAAAAAAGTACTACTAT+TGG - chr3_2:70874849-70874868 None:intergenic 25.0%
!!! TGTTTTGGAACTGTATTTTT+TGG + chr3_2:70874707-70874726 Msa0421830:three_prime_UTR 25.0%
! TATTGTCTCATTTATTACTG+TGG + chr3_2:70874766-70874785 Msa0421830:three_prime_UTR 25.0%
!! AAAGTACTACTATTGGTAAA+AGG - chr3_2:70874842-70874861 None:intergenic 25.0%
!!! ACGTAAAAAAGTACTACTAT+TGG - chr3_2:70874849-70874868 None:intergenic 25.0%
! TTTGATAGTTGAAGTGTTTG+AGG - chr3_2:70873338-70873357 None:intergenic 30.0%
!!! AAATCATGAGAAGTGTTTTG+TGG - chr3_2:70873404-70873423 None:intergenic 30.0%
ATAGAGAGAACTATATCATC+AGG - chr3_2:70873440-70873459 None:intergenic 30.0%
GATATAGTTCTCTCTATACT+TGG + chr3_2:70873443-70873462 Msa0421830:CDS 30.0%
AAAATCTGAAGGAGAATTTG+CGG - chr3_2:70873483-70873502 None:intergenic 30.0%
AGAACACTGATAAAATCTGA+AGG - chr3_2:70873494-70873513 None:intergenic 30.0%
AACAGATGCAAAAGACTAAA+CGG + chr3_2:70873607-70873626 Msa0421830:intron 30.0%
!! TAATGTTTTGTTCGAAGCTT+TGG - chr3_2:70873659-70873678 None:intergenic 30.0%
TATCAGTCAAACCTAAAAAC+TGG + chr3_2:70873677-70873696 Msa0421830:CDS 30.0%
! TTTTACTGTTTGCAAAACAG+AGG + chr3_2:70873890-70873909 Msa0421830:CDS 30.0%
GTTAAACAGAACGTTATTGA+AGG + chr3_2:70874121-70874140 Msa0421830:CDS 30.0%
GTTATTGCCGTTAGATAAAA+CGG - chr3_2:70874188-70874207 None:intergenic 30.0%
TTTCAAAATGTTAACGGTGA+CGG + chr3_2:70874673-70874692 Msa0421830:three_prime_UTR 30.0%
! TTTGATAGTTGAAGTGTTTG+AGG - chr3_2:70873338-70873357 None:intergenic 30.0%
!!! AAATCATGAGAAGTGTTTTG+TGG - chr3_2:70873404-70873423 None:intergenic 30.0%
ATAGAGAGAACTATATCATC+AGG - chr3_2:70873440-70873459 None:intergenic 30.0%
GATATAGTTCTCTCTATACT+TGG + chr3_2:70873443-70873462 Msa0421830:CDS 30.0%
AAAATCTGAAGGAGAATTTG+CGG - chr3_2:70873483-70873502 None:intergenic 30.0%
AGAACACTGATAAAATCTGA+AGG - chr3_2:70873494-70873513 None:intergenic 30.0%
AACAGATGCAAAAGACTAAA+CGG + chr3_2:70873607-70873626 Msa0421830:intron 30.0%
!! TAATGTTTTGTTCGAAGCTT+TGG - chr3_2:70873659-70873678 None:intergenic 30.0%
TATCAGTCAAACCTAAAAAC+TGG + chr3_2:70873677-70873696 Msa0421830:CDS 30.0%
! TTTTACTGTTTGCAAAACAG+AGG + chr3_2:70873890-70873909 Msa0421830:CDS 30.0%
GTTAAACAGAACGTTATTGA+AGG + chr3_2:70874121-70874140 Msa0421830:CDS 30.0%
GTTATTGCCGTTAGATAAAA+CGG - chr3_2:70874188-70874207 None:intergenic 30.0%
TTTCAAAATGTTAACGGTGA+CGG + chr3_2:70874673-70874692 Msa0421830:three_prime_UTR 30.0%
ATTATTATGCGAACCAGAAG+AGG + chr3_2:70873302-70873321 Msa0421830:exon 35.0%
ACTGCCAATGAATACAAAGA+CGG - chr3_2:70873962-70873981 None:intergenic 35.0%
CTTGGTCATTGTTTACAAGA+CGG + chr3_2:70874091-70874110 Msa0421830:CDS 35.0%
CATTGTTTACAAGACGGTTA+CGG + chr3_2:70874097-70874116 Msa0421830:CDS 35.0%
!!! TGATTTTGGTTGTAACGTTC+CGG + chr3_2:70874321-70874340 Msa0421830:CDS 35.0%
ATGAGGTTATGTTCGAATTC+TGG + chr3_2:70874418-70874437 Msa0421830:CDS 35.0%
! TTATGTTCGAATTCTGGTTG+TGG + chr3_2:70874424-70874443 Msa0421830:CDS 35.0%
ACATGAGTTTCGAAGATGTT+CGG + chr3_2:70874465-70874484 Msa0421830:CDS 35.0%
!!! GGAACTGTATTTTTTGGCAT+TGG + chr3_2:70874713-70874732 Msa0421830:three_prime_UTR 35.0%
AATGCAATTGCAAAGTCCAA+TGG + chr3_2:70874797-70874816 Msa0421830:three_prime_UTR 35.0%
ACTATTGGTAAAAGGAACAG+AGG - chr3_2:70874834-70874853 None:intergenic 35.0%
ATTATTATGCGAACCAGAAG+AGG + chr3_2:70873302-70873321 Msa0421830:exon 35.0%
ACTGCCAATGAATACAAAGA+CGG - chr3_2:70873962-70873981 None:intergenic 35.0%
CTTGGTCATTGTTTACAAGA+CGG + chr3_2:70874091-70874110 Msa0421830:CDS 35.0%
CATTGTTTACAAGACGGTTA+CGG + chr3_2:70874097-70874116 Msa0421830:CDS 35.0%
!!! TGATTTTGGTTGTAACGTTC+CGG + chr3_2:70874321-70874340 Msa0421830:CDS 35.0%
ATGAGGTTATGTTCGAATTC+TGG + chr3_2:70874418-70874437 Msa0421830:CDS 35.0%
! TTATGTTCGAATTCTGGTTG+TGG + chr3_2:70874424-70874443 Msa0421830:CDS 35.0%
ACATGAGTTTCGAAGATGTT+CGG + chr3_2:70874465-70874484 Msa0421830:CDS 35.0%
!!! GGAACTGTATTTTTTGGCAT+TGG + chr3_2:70874713-70874732 Msa0421830:three_prime_UTR 35.0%
AATGCAATTGCAAAGTCCAA+TGG + chr3_2:70874797-70874816 Msa0421830:three_prime_UTR 35.0%
ACTATTGGTAAAAGGAACAG+AGG - chr3_2:70874834-70874853 None:intergenic 35.0%
! AAGGGTAAAAGCATAGAGAG+GGG - chr3_2:70873267-70873286 None:intergenic 40.0%
! GAAGGGTAAAAGCATAGAGA+GGG - chr3_2:70873268-70873287 None:intergenic 40.0%
! AGAAGGGTAAAAGCATAGAG+AGG - chr3_2:70873269-70873288 None:intergenic 40.0%
AGGATAACACAAACCTCTTC+TGG - chr3_2:70873318-70873337 None:intergenic 40.0%
! GCAAAGTCACACCAGTTTTT+AGG - chr3_2:70873691-70873710 None:intergenic 40.0%
CAACACAGCGTTTGAGAAAA+CGG - chr3_2:70873717-70873736 None:intergenic 40.0%
ATTGAAGCCTGTTACACTCT+TGG + chr3_2:70873748-70873767 Msa0421830:CDS 40.0%
! TACCATTCCAAGAGTGTAAC+AGG - chr3_2:70873758-70873777 None:intergenic 40.0%
TGTTTGCAAAACAGAGGAAG+CGG + chr3_2:70873896-70873915 Msa0421830:CDS 40.0%
TTGGCAGTGATTCAGTTCAA+TGG + chr3_2:70873974-70873993 Msa0421830:CDS 40.0%
GTGATTCAGTTCAATGGAAG+TGG + chr3_2:70873980-70873999 Msa0421830:CDS 40.0%
ATTCAGTTCAATGGAAGTGG+AGG + chr3_2:70873983-70874002 Msa0421830:CDS 40.0%
AGAACGTTATTGAAGGTCGA+CGG + chr3_2:70874128-70874147 Msa0421830:CDS 40.0%
!!! CTTCGTGTTTTAGCTGTTTC+TGG + chr3_2:70874265-70874284 Msa0421830:CDS 40.0%
!! TTCTGGTTCTGTTTCTGGTT+CGG + chr3_2:70874282-70874301 Msa0421830:CDS 40.0%
! TGTCCTTTGCTGAGTGATTT+TGG + chr3_2:70874307-70874326 Msa0421830:CDS 40.0%
CAACCAAAATCACTCAGCAA+AGG - chr3_2:70874313-70874332 None:intergenic 40.0%
TTCGAACATAACCTCATACC+CGG - chr3_2:70874415-70874434 None:intergenic 40.0%
AACTCATGTTTCCTTGTCTC+AGG - chr3_2:70874454-70874473 None:intergenic 40.0%
! TTTCGAAGATGTTCGGTTTG+TGG + chr3_2:70874472-70874491 Msa0421830:CDS 40.0%
TGATTGGAAGTTTCGACACA+AGG + chr3_2:70874531-70874550 Msa0421830:CDS 40.0%
! TGGTGTTAACATTGATGAGG+TGG + chr3_2:70874618-70874637 Msa0421830:CDS 40.0%
! AATGTTAACGGTGACGGTAA+CGG + chr3_2:70874679-70874698 Msa0421830:three_prime_UTR 40.0%
!!! ACGGTAACGGAAGATTGTTT+TGG + chr3_2:70874692-70874711 Msa0421830:three_prime_UTR 40.0%
! TGGCATTGGATACTGAAACA+TGG + chr3_2:70874727-70874746 Msa0421830:three_prime_UTR 40.0%
! AAGGGTAAAAGCATAGAGAG+GGG - chr3_2:70873267-70873286 None:intergenic 40.0%
! GAAGGGTAAAAGCATAGAGA+GGG - chr3_2:70873268-70873287 None:intergenic 40.0%
! AGAAGGGTAAAAGCATAGAG+AGG - chr3_2:70873269-70873288 None:intergenic 40.0%
AGGATAACACAAACCTCTTC+TGG - chr3_2:70873318-70873337 None:intergenic 40.0%
! GCAAAGTCACACCAGTTTTT+AGG - chr3_2:70873691-70873710 None:intergenic 40.0%
CAACACAGCGTTTGAGAAAA+CGG - chr3_2:70873717-70873736 None:intergenic 40.0%
ATTGAAGCCTGTTACACTCT+TGG + chr3_2:70873748-70873767 Msa0421830:CDS 40.0%
! TACCATTCCAAGAGTGTAAC+AGG - chr3_2:70873758-70873777 None:intergenic 40.0%
TGTTTGCAAAACAGAGGAAG+CGG + chr3_2:70873896-70873915 Msa0421830:CDS 40.0%
TTGGCAGTGATTCAGTTCAA+TGG + chr3_2:70873974-70873993 Msa0421830:CDS 40.0%
GTGATTCAGTTCAATGGAAG+TGG + chr3_2:70873980-70873999 Msa0421830:CDS 40.0%
ATTCAGTTCAATGGAAGTGG+AGG + chr3_2:70873983-70874002 Msa0421830:CDS 40.0%
AGAACGTTATTGAAGGTCGA+CGG + chr3_2:70874128-70874147 Msa0421830:CDS 40.0%
!!! CTTCGTGTTTTAGCTGTTTC+TGG + chr3_2:70874265-70874284 Msa0421830:CDS 40.0%
!! TTCTGGTTCTGTTTCTGGTT+CGG + chr3_2:70874282-70874301 Msa0421830:CDS 40.0%
! TGTCCTTTGCTGAGTGATTT+TGG + chr3_2:70874307-70874326 Msa0421830:CDS 40.0%
CAACCAAAATCACTCAGCAA+AGG - chr3_2:70874313-70874332 None:intergenic 40.0%
TTCGAACATAACCTCATACC+CGG - chr3_2:70874415-70874434 None:intergenic 40.0%
AACTCATGTTTCCTTGTCTC+AGG - chr3_2:70874454-70874473 None:intergenic 40.0%
! TTTCGAAGATGTTCGGTTTG+TGG + chr3_2:70874472-70874491 Msa0421830:CDS 40.0%
TGATTGGAAGTTTCGACACA+AGG + chr3_2:70874531-70874550 Msa0421830:CDS 40.0%
! TGGTGTTAACATTGATGAGG+TGG + chr3_2:70874618-70874637 Msa0421830:CDS 40.0%
! AATGTTAACGGTGACGGTAA+CGG + chr3_2:70874679-70874698 Msa0421830:three_prime_UTR 40.0%
!!! ACGGTAACGGAAGATTGTTT+TGG + chr3_2:70874692-70874711 Msa0421830:three_prime_UTR 40.0%
! TGGCATTGGATACTGAAACA+TGG + chr3_2:70874727-70874746 Msa0421830:three_prime_UTR 40.0%
ATCTGAAGGAGAATTTGCGG+TGG - chr3_2:70873480-70873499 None:intergenic 45.0%
AGCCTGTTACACTCTTGGAA+TGG + chr3_2:70873753-70873772 Msa0421830:CDS 45.0%
TTGTCGTGTGTCTGCAGATT+CGG + chr3_2:70873867-70873886 Msa0421830:intron 45.0%
!!! CTCGCTGCCGTTTTATCTAA+CGG + chr3_2:70874178-70874197 Msa0421830:CDS 45.0%
TTAACACTCCACGTCAGCAA+CGG - chr3_2:70874220-70874239 None:intergenic 45.0%
ACAGCTAAAACACGAAGCTG+AGG - chr3_2:70874262-70874281 None:intergenic 45.0%
!! GCTGTTTCTGGTTCTGTTTC+TGG + chr3_2:70874277-70874296 Msa0421830:CDS 45.0%
!! TCTGGTTCTGTTTCTGGTTC+GGG + chr3_2:70874283-70874302 Msa0421830:CDS 45.0%
! GTTCGAATTCTGGTTGTGGA+AGG + chr3_2:70874428-70874447 Msa0421830:CDS 45.0%
TTGGAAGTTTCGACACAAGG+CGG + chr3_2:70874534-70874553 Msa0421830:CDS 45.0%
! CGGTGGTGTTAACATTGATG+AGG + chr3_2:70874615-70874634 Msa0421830:CDS 45.0%
! GGTTGTGCTTGATAGCTGAT+AGG + chr3_2:70874639-70874658 Msa0421830:exon 45.0%
! TGCAAAGTCCAATGGTAGTG+TGG + chr3_2:70874805-70874824 Msa0421830:three_prime_UTR 45.0%
ATCTGAAGGAGAATTTGCGG+TGG - chr3_2:70873480-70873499 None:intergenic 45.0%
AGCCTGTTACACTCTTGGAA+TGG + chr3_2:70873753-70873772 Msa0421830:CDS 45.0%
TTGTCGTGTGTCTGCAGATT+CGG + chr3_2:70873867-70873886 Msa0421830:intron 45.0%
!!! CTCGCTGCCGTTTTATCTAA+CGG + chr3_2:70874178-70874197 Msa0421830:CDS 45.0%
TTAACACTCCACGTCAGCAA+CGG - chr3_2:70874220-70874239 None:intergenic 45.0%
ACAGCTAAAACACGAAGCTG+AGG - chr3_2:70874262-70874281 None:intergenic 45.0%
!! GCTGTTTCTGGTTCTGTTTC+TGG + chr3_2:70874277-70874296 Msa0421830:CDS 45.0%
!! TCTGGTTCTGTTTCTGGTTC+GGG + chr3_2:70874283-70874302 Msa0421830:CDS 45.0%
! GTTCGAATTCTGGTTGTGGA+AGG + chr3_2:70874428-70874447 Msa0421830:CDS 45.0%
TTGGAAGTTTCGACACAAGG+CGG + chr3_2:70874534-70874553 Msa0421830:CDS 45.0%
! CGGTGGTGTTAACATTGATG+AGG + chr3_2:70874615-70874634 Msa0421830:CDS 45.0%
! GGTTGTGCTTGATAGCTGAT+AGG + chr3_2:70874639-70874658 Msa0421830:exon 45.0%
! TGCAAAGTCCAATGGTAGTG+TGG + chr3_2:70874805-70874824 Msa0421830:three_prime_UTR 45.0%
! CTCAAACGCTGTGTTGATGC+TGG + chr3_2:70873721-70873740 Msa0421830:CDS 50.0%
CGCGCCGTCTTTGTATTCAT+TGG + chr3_2:70873955-70873974 Msa0421830:CDS 50.0%
AACGACAAAGACTTACGCGC+CGG + chr3_2:70874013-70874032 Msa0421830:CDS 50.0%
GCATTTCTCGGTCACATCGA+CGG + chr3_2:70874058-70874077 Msa0421830:CDS 50.0%
ATCGACGGTTTACGTGAGCT+TGG + chr3_2:70874073-70874092 Msa0421830:CDS 50.0%
ACAAACAACCGTTGCTGACG+TGG + chr3_2:70874209-70874228 Msa0421830:CDS 50.0%
!! TGAGGTGGTTGGTTTGAACC+CGG - chr3_2:70874244-70874263 None:intergenic 50.0%
AAACACGAAGCTGAGGTGGT+TGG - chr3_2:70874255-70874274 None:intergenic 50.0%
GCTAAAACACGAAGCTGAGG+TGG - chr3_2:70874259-70874278 None:intergenic 50.0%
TGGTTGTAACGTTCCGGTTC+AGG + chr3_2:70874327-70874346 Msa0421830:CDS 50.0%
!! CCATCATAAACCGGTTCGCT+GGG - chr3_2:70874360-70874379 None:intergenic 50.0%
! CCAGCGAACCGGTTTATGAT+GGG + chr3_2:70874358-70874377 Msa0421830:CDS 50.0%
! CAGCGAACCGGTTTATGATG+GGG + chr3_2:70874359-70874378 Msa0421830:CDS 50.0%
CGAACCACCCCATCATAAAC+CGG - chr3_2:70874369-70874388 None:intergenic 50.0%
ATGGGGTGGTTCGATATTCG+AGG + chr3_2:70874376-70874395 Msa0421830:CDS 50.0%
AGAGGAGACCACACTACCAT+TGG - chr3_2:70874816-70874835 None:intergenic 50.0%
! CTCAAACGCTGTGTTGATGC+TGG + chr3_2:70873721-70873740 Msa0421830:CDS 50.0%
CGCGCCGTCTTTGTATTCAT+TGG + chr3_2:70873955-70873974 Msa0421830:CDS 50.0%
AACGACAAAGACTTACGCGC+CGG + chr3_2:70874013-70874032 Msa0421830:CDS 50.0%
GCATTTCTCGGTCACATCGA+CGG + chr3_2:70874058-70874077 Msa0421830:CDS 50.0%
ATCGACGGTTTACGTGAGCT+TGG + chr3_2:70874073-70874092 Msa0421830:CDS 50.0%
ACAAACAACCGTTGCTGACG+TGG + chr3_2:70874209-70874228 Msa0421830:CDS 50.0%
!! TGAGGTGGTTGGTTTGAACC+CGG - chr3_2:70874244-70874263 None:intergenic 50.0%
AAACACGAAGCTGAGGTGGT+TGG - chr3_2:70874255-70874274 None:intergenic 50.0%
GCTAAAACACGAAGCTGAGG+TGG - chr3_2:70874259-70874278 None:intergenic 50.0%
TGGTTGTAACGTTCCGGTTC+AGG + chr3_2:70874327-70874346 Msa0421830:CDS 50.0%
!! CCATCATAAACCGGTTCGCT+GGG - chr3_2:70874360-70874379 None:intergenic 50.0%
! CCAGCGAACCGGTTTATGAT+GGG + chr3_2:70874358-70874377 Msa0421830:CDS 50.0%
! CAGCGAACCGGTTTATGATG+GGG + chr3_2:70874359-70874378 Msa0421830:CDS 50.0%
CGAACCACCCCATCATAAAC+CGG - chr3_2:70874369-70874388 None:intergenic 50.0%
ATGGGGTGGTTCGATATTCG+AGG + chr3_2:70874376-70874395 Msa0421830:CDS 50.0%
AGAGGAGACCACACTACCAT+TGG - chr3_2:70874816-70874835 None:intergenic 50.0%
GCGTGCACATAAAGCCACAC+CGG - chr3_2:70874035-70874054 None:intergenic 55.0%
TGTGCACGCGCTGCATTTCT+CGG + chr3_2:70874046-70874065 Msa0421830:CDS 55.0%
! GCTGACGTGGAGTGTTAACC+CGG + chr3_2:70874222-70874241 Msa0421830:CDS 55.0%
! CTGACGTGGAGTGTTAACCC+GGG + chr3_2:70874223-70874242 Msa0421830:CDS 55.0%
!! GAGGTGGTTGGTTTGAACCC+GGG - chr3_2:70874243-70874262 None:intergenic 55.0%
! CCCAGCGAACCGGTTTATGA+TGG + chr3_2:70874357-70874376 Msa0421830:CDS 55.0%
!! CCCATCATAAACCGGTTCGC+TGG - chr3_2:70874361-70874380 None:intergenic 55.0%
! CGAACCGGTTTATGATGGGG+TGG + chr3_2:70874362-70874381 Msa0421830:CDS 55.0%
GGGTGGTTCGATATTCGAGG+CGG + chr3_2:70874379-70874398 Msa0421830:CDS 55.0%
GGTGGTTCGATATTCGAGGC+GGG + chr3_2:70874380-70874399 Msa0421830:CDS 55.0%
! GATATTCGAGGCGGGTTTCC+TGG + chr3_2:70874388-70874407 Msa0421830:CDS 55.0%
CATAACCTCATACCCGGTCC+AGG - chr3_2:70874409-70874428 None:intergenic 55.0%
GTTGTGGAAGGCCTGAGACA+AGG + chr3_2:70874440-70874459 Msa0421830:CDS 55.0%
GCGCTTGTCAAGCGCTTGAT+TGG + chr3_2:70874515-70874534 Msa0421830:CDS 55.0%
TCCTCTAACCACCTCTCCAC+TGG - chr3_2:70874568-70874587 None:intergenic 55.0%
AGTGGAGAGGTGGTTAGAGG+AGG + chr3_2:70874567-70874586 Msa0421830:CDS 55.0%
GAGGAGGAGGAACATGTTGC+CGG + chr3_2:70874583-70874602 Msa0421830:CDS 55.0%
GAGGAACATGTTGCCGGTGA+AGG + chr3_2:70874589-70874608 Msa0421830:CDS 55.0%
CATGTTGCCGGTGAAGGTGA+CGG + chr3_2:70874595-70874614 Msa0421830:CDS 55.0%
AACACCACCGTCACCTTCAC+CGG - chr3_2:70874605-70874624 None:intergenic 55.0%
GCGTGCACATAAAGCCACAC+CGG - chr3_2:70874035-70874054 None:intergenic 55.0%
TGTGCACGCGCTGCATTTCT+CGG + chr3_2:70874046-70874065 Msa0421830:CDS 55.0%
! GCTGACGTGGAGTGTTAACC+CGG + chr3_2:70874222-70874241 Msa0421830:CDS 55.0%
! CTGACGTGGAGTGTTAACCC+GGG + chr3_2:70874223-70874242 Msa0421830:CDS 55.0%
!! GAGGTGGTTGGTTTGAACCC+GGG - chr3_2:70874243-70874262 None:intergenic 55.0%
! CCCAGCGAACCGGTTTATGA+TGG + chr3_2:70874357-70874376 Msa0421830:CDS 55.0%
!! CCCATCATAAACCGGTTCGC+TGG - chr3_2:70874361-70874380 None:intergenic 55.0%
! CGAACCGGTTTATGATGGGG+TGG + chr3_2:70874362-70874381 Msa0421830:CDS 55.0%
GGGTGGTTCGATATTCGAGG+CGG + chr3_2:70874379-70874398 Msa0421830:CDS 55.0%
GGTGGTTCGATATTCGAGGC+GGG + chr3_2:70874380-70874399 Msa0421830:CDS 55.0%
! GATATTCGAGGCGGGTTTCC+TGG + chr3_2:70874388-70874407 Msa0421830:CDS 55.0%
CATAACCTCATACCCGGTCC+AGG - chr3_2:70874409-70874428 None:intergenic 55.0%
GTTGTGGAAGGCCTGAGACA+AGG + chr3_2:70874440-70874459 Msa0421830:CDS 55.0%
GCGCTTGTCAAGCGCTTGAT+TGG + chr3_2:70874515-70874534 Msa0421830:CDS 55.0%
TCCTCTAACCACCTCTCCAC+TGG - chr3_2:70874568-70874587 None:intergenic 55.0%
AGTGGAGAGGTGGTTAGAGG+AGG + chr3_2:70874567-70874586 Msa0421830:CDS 55.0%
GAGGAGGAGGAACATGTTGC+CGG + chr3_2:70874583-70874602 Msa0421830:CDS 55.0%
GAGGAACATGTTGCCGGTGA+AGG + chr3_2:70874589-70874608 Msa0421830:CDS 55.0%
CATGTTGCCGGTGAAGGTGA+CGG + chr3_2:70874595-70874614 Msa0421830:CDS 55.0%
AACACCACCGTCACCTTCAC+CGG - chr3_2:70874605-70874624 None:intergenic 55.0%
! CAAAGACTTACGCGCCGGTG+TGG + chr3_2:70874018-70874037 Msa0421830:CDS 60.0%
GCTGGGTGAGTCTCCTGAAC+CGG - chr3_2:70874343-70874362 None:intergenic 60.0%
AGGAGACTCACCCAGCGAAC+CGG + chr3_2:70874347-70874366 Msa0421830:CDS 60.0%
GGTTTCCTGGACCGGGTATG+AGG + chr3_2:70874401-70874420 Msa0421830:CDS 60.0%
GCCAGTGGAGAGGTGGTTAG+AGG + chr3_2:70874564-70874583 Msa0421830:CDS 60.0%
GGAGAGGTGGTTAGAGGAGG+AGG + chr3_2:70874570-70874589 Msa0421830:CDS 60.0%
! CAAAGACTTACGCGCCGGTG+TGG + chr3_2:70874018-70874037 Msa0421830:CDS 60.0%
GCTGGGTGAGTCTCCTGAAC+CGG - chr3_2:70874343-70874362 None:intergenic 60.0%
AGGAGACTCACCCAGCGAAC+CGG + chr3_2:70874347-70874366 Msa0421830:CDS 60.0%
GGTTTCCTGGACCGGGTATG+AGG + chr3_2:70874401-70874420 Msa0421830:CDS 60.0%
GCCAGTGGAGAGGTGGTTAG+AGG + chr3_2:70874564-70874583 Msa0421830:CDS 60.0%
GGAGAGGTGGTTAGAGGAGG+AGG + chr3_2:70874570-70874589 Msa0421830:CDS 60.0%
AGGAAGCGGCGCGTCGTTGA+TGG + chr3_2:70873910-70873929 Msa0421830:CDS 65.0%
! TCGAGGCGGGTTTCCTGGAC+CGG + chr3_2:70874393-70874412 Msa0421830:CDS 65.0%
AGTGTGCGCCAGTGGAGAGG+TGG + chr3_2:70874557-70874576 Msa0421830:CDS 65.0%
! GTTGCCGGTGAAGGTGACGG+TGG + chr3_2:70874598-70874617 Msa0421830:CDS 65.0%
AGGAAGCGGCGCGTCGTTGA+TGG + chr3_2:70873910-70873929 Msa0421830:CDS 65.0%
! TCGAGGCGGGTTTCCTGGAC+CGG + chr3_2:70874393-70874412 Msa0421830:CDS 65.0%
AGTGTGCGCCAGTGGAGAGG+TGG + chr3_2:70874557-70874576 Msa0421830:CDS 65.0%
! GTTGCCGGTGAAGGTGACGG+TGG + chr3_2:70874598-70874617 Msa0421830:CDS 65.0%
! CGAGGCGGGTTTCCTGGACC+GGG + chr3_2:70874394-70874413 Msa0421830:CDS 70.0%
CAAGGCGGAGTGTGCGCCAG+TGG + chr3_2:70874549-70874568 Msa0421830:CDS 70.0%
CGGAGTGTGCGCCAGTGGAG+AGG + chr3_2:70874554-70874573 Msa0421830:CDS 70.0%
! CGAGGCGGGTTTCCTGGACC+GGG + chr3_2:70874394-70874413 Msa0421830:CDS 70.0%
CAAGGCGGAGTGTGCGCCAG+TGG + chr3_2:70874549-70874568 Msa0421830:CDS 70.0%
CGGAGTGTGCGCCAGTGGAG+AGG + chr3_2:70874554-70874573 Msa0421830:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 70873260 70874921 70873260 ID=Msa0421830;Name=Msa0421830
chr3_2 mRNA 70873260 70874921 70873260 ID=Msa0421830-mRNA-1;Parent=Msa0421830;Name=Msa0421830-mRNA-1;_AED=0.04;_eAED=0.04;_QI=48|1|1|1|1|1|3|264|382
chr3_2 exon 70873260 70873519 70873260 ID=Msa0421830-mRNA-1:exon:17111;Parent=Msa0421830-mRNA-1
chr3_2 exon 70873612 70873774 70873612 ID=Msa0421830-mRNA-1:exon:17112;Parent=Msa0421830-mRNA-1
chr3_2 exon 70873884 70874921 70873884 ID=Msa0421830-mRNA-1:exon:17113;Parent=Msa0421830-mRNA-1
chr3_2 five_prime_UTR 70873260 70873307 70873260 ID=Msa0421830-mRNA-1:five_prime_utr;Parent=Msa0421830-mRNA-1
chr3_2 CDS 70873308 70873519 70873308 ID=Msa0421830-mRNA-1:cds;Parent=Msa0421830-mRNA-1
chr3_2 CDS 70873612 70873774 70873612 ID=Msa0421830-mRNA-1:cds;Parent=Msa0421830-mRNA-1
chr3_2 CDS 70873884 70874657 70873884 ID=Msa0421830-mRNA-1:cds;Parent=Msa0421830-mRNA-1
chr3_2 three_prime_UTR 70874658 70874921 70874658 ID=Msa0421830-mRNA-1:three_prime_utr;Parent=Msa0421830-mRNA-1
Gene Sequence

>Msa0421830

ATGCGAACCAGAAGAGGTTTGTGTTATCCTCAAACACTTCAACTATCAAACATGTTTTCAGACACAAACAATAACAAAAGACACAAGAGAATGCCACAAAACACTTCTCATGATTTCTTTGAACTTTTGCCTGATGATATAGTTCTCTCTATACTTGGAAAACTCAGCTCCACCGCAAATTCTCCTTCAGATTTTATCAGTGTTCTAATCACATGCAAAAGACTAAACGGCTTAAGTCTTAACTCTGTTGTTTTGTCCAAAGCTTCGAACAAAACATTATCAGTCAAACCTAAAAACTGGTGTGACTTTGCTCACCGTTTTCTCAAACGCTGTGTTGATGCTGGAAACATTGAAGCCTGTTACACTCTTGGAATGATTCGGTTTTACTGTTTGCAAAACAGAGGAAGCGGCGCGTCGTTGATGGCAAAAGCAGCTATGAAATCTCACGCGCCGTCTTTGTATTCATTGGCAGTGATTCAGTTCAATGGAAGTGGAGGAACAAAAAACGACAAAGACTTACGCGCCGGTGTGGCTTTATGTGCACGCGCTGCATTTCTCGGTCACATCGACGGTTTACGTGAGCTTGGTCATTGTTTACAAGACGGTTACGGCGTTAAACAGAACGTTATTGAAGGTCGACGGTTTCTAGTTCAAGCTAATGCTCGTGAACTCGCTGCCGTTTTATCTAACGGCAATAACAACAAACAACCGTTGCTGACGTGGAGTGTTAACCCGGGTTCAAACCAACCACCTCAGCTTCGTGTTTTAGCTGTTTCTGGTTCTGTTTCTGGTTCGGGATGTCCTTTGCTGAGTGATTTTGGTTGTAACGTTCCGGTTCAGGAGACTCACCCAGCGAACCGGTTTATGATGGGGTGGTTCGATATTCGAGGCGGGTTTCCTGGACCGGGTATGAGGTTATGTTCGAATTCTGGTTGTGGAAGGCCTGAGACAAGGAAACATGAGTTTCGAAGATGTTCGGTTTGTGGTGTTGTGAATTATTGTTCACGCGCTTGTCAAGCGCTTGATTGGAAGTTTCGACACAAGGCGGAGTGTGCGCCAGTGGAGAGGTGGTTAGAGGAGGAGGAACATGTTGCCGGTGAAGGTGACGGTGGTGTTAACATTGATGAGGTGGTTGTGCTTGATAGCTGA
Protein sequence

>Msa0421830

MRTRRGLCYPQTLQLSNMFSDTNNNKRHKRMPQNTSHDFFELLPDDIVLSILGKLSSTANSPSDFISVLITCKRLNGLSLNSVVLSKASNKTLSVKPKNWCDFAHRFLKRCVDAGNIEACYTLGMIRFYCLQNRGSGASLMAKAAMKSHAPSLYSLAVIQFNGSGGTKNDKDLRAGVALCARAAFLGHIDGLRELGHCLQDGYGVKQNVIEGRRFLVQANARELAAVLSNGNNNKQPLLTWSVNPGSNQPPQLRVLAVSGSVSGSGCPLLSDFGCNVPVQETHPANRFMMGWFDIRGGFPGPGMRLCSNSGCGRPETRKHEFRRCSVCGVVNYCSRACQALDWKFRHKAECAPVERWLEEEEHVAGEGDGGVNIDEVVVLDS*