AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0422490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0422490 MtrunA17_Chr3g0117391 97.638 127 3 0 1 127 1 127 1.70e-91 260
Msa0422490 MtrunA17_Chr3g0117411 81.667 120 19 1 1 120 1 117 1.71e-70 207
Msa0422490 MtrunA17_Chr7g0228311 59.048 105 43 0 21 125 3 107 3.91e-44 139
Msa0422490 MtrunA17_Chr1g0182971 48.000 100 49 2 25 123 87 184 7.18e-26 95.9
Msa0422490 MtrunA17_Chr2g0299021 42.000 100 51 1 15 114 84 176 9.28e-23 87.8
Msa0422490 MtrunA17_Chr5g0404751 42.353 85 49 0 30 114 42 126 1.50e-19 78.2
Msa0422490 MtrunA17_Chr2g0286251 38.000 100 62 0 21 120 1 100 1.57e-18 74.7
Msa0422490 MtrunA17_Chr7g0222541 39.423 104 62 1 18 120 27 130 6.19e-18 73.9
Msa0422490 MtrunA17_Chr1g0195951 34.000 100 66 0 21 120 1 100 6.85e-18 73.2
Msa0422490 MtrunA17_Chr7g0222551 40.385 104 61 1 18 120 27 130 7.12e-18 73.9
Msa0422490 MtrunA17_Chr7g0222491 39.423 104 62 1 18 120 27 130 1.22e-17 73.2
Msa0422490 MtrunA17_Chr7g0224091 37.615 109 67 1 13 120 14 122 4.04e-17 71.6
Msa0422490 MtrunA17_Chr7g0268751 34.000 100 66 0 21 120 1 100 1.09e-16 70.1
Msa0422490 MtrunA17_Chr4g0066791 35.644 101 64 1 21 120 1 101 8.66e-16 67.8
Msa0422490 MtrunA17_Chr4g0066801 35.644 101 64 1 21 120 1 101 8.66e-16 67.8
Msa0422490 MtrunA17_Chr4g0066811 35.644 101 64 1 21 120 1 101 8.66e-16 67.8
Msa0422490 MtrunA17_Chr7g0268731 36.634 101 63 1 21 120 1 101 9.14e-16 67.8
Msa0422490 MtrunA17_Chr1g0195991 34.043 94 62 0 21 114 1 94 1.84e-15 67.0
Msa0422490 MtrunA17_Chr1g0195981 34.043 94 62 0 21 114 1 94 2.03e-15 66.6
Msa0422490 MtrunA17_Chr7g0268721 35.644 101 64 1 21 120 1 101 4.66e-15 65.9
Msa0422490 MtrunA17_Chr2g0303741 35.354 99 60 1 26 120 44 142 5.70e-15 66.6
Msa0422490 MtrunA17_Chr1g0195971 35.000 100 65 0 21 120 1 100 8.41e-15 65.1
Msa0422490 MtrunA17_Chr2g0286271 32.000 100 68 0 21 120 1 100 1.46e-14 64.7
Msa0422490 MtrunA17_Chr7g0268761 35.106 94 61 0 27 120 7 100 2.56e-14 63.9
Msa0422490 MtrunA17_Chr1g0195931 35.106 94 61 0 27 120 7 100 1.13e-13 62.4
Msa0422490 MtrunA17_Chr5g0434201 40.476 84 48 1 39 120 38 121 1.36e-13 62.8
Msa0422490 MtrunA17_Chr7g0268711 31.915 94 64 0 21 114 1 94 1.77e-12 59.3
Msa0422490 MtrunA17_Chr2g0282951 35.000 100 62 3 22 120 28 125 2.51e-12 59.3
Msa0422490 MtrunA17_Chr2g0325001 35.135 111 63 3 13 120 34 138 3.31e-11 57.0
Msa0422490 MtrunA17_Chr1g0151231 31.132 106 69 2 18 120 43 147 6.42e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0422490 AT5G63030.1 65.354 127 42 1 1 127 1 125 5.26e-59 178
Msa0422490 AT5G40370.1 62.857 105 39 0 21 125 3 107 4.99e-47 147
Msa0422490 AT5G40370.2 64.835 91 32 0 35 125 42 132 4.34e-41 133
Msa0422490 AT2G20270.1 48.000 100 50 2 20 117 73 172 2.41e-24 92.0
Msa0422490 AT5G20500.1 42.105 95 55 0 24 118 35 129 3.29e-23 88.2
Msa0422490 AT1G77370.1 46.512 86 46 0 30 115 43 128 2.84e-22 85.5
Msa0422490 AT4G28730.1 41.748 103 56 2 16 117 68 167 2.97e-21 84.0
Msa0422490 AT5G14070.1 38.532 109 59 1 20 120 30 138 1.69e-19 78.6
Msa0422490 AT2G20270.2 37.795 127 50 2 20 117 73 199 3.41e-19 79.7
Msa0422490 AT5G18600.1 38.000 100 62 0 21 120 1 100 1.89e-17 72.4
Msa0422490 AT1G77370.2 36.036 111 45 1 30 114 43 153 7.05e-17 72.4
Msa0422490 AT1G03020.1 39.000 100 61 0 21 120 1 100 1.30e-16 70.1
Msa0422490 AT3G02000.1 36.697 109 63 1 18 120 26 134 5.54e-16 69.7
Msa0422490 AT4G15700.1 39.394 99 59 1 22 120 3 100 1.00e-15 67.8
Msa0422490 AT4G33040.1 32.203 118 76 1 7 120 25 142 1.63e-15 68.6
Msa0422490 AT3G62930.1 35.000 100 65 0 21 120 1 100 1.66e-15 67.4
Msa0422490 AT4G15670.1 34.000 100 66 0 21 120 1 100 5.17e-15 66.2
Msa0422490 AT4G15680.1 33.000 100 67 0 21 120 1 100 9.88e-15 65.5
Msa0422490 AT4G15690.1 37.374 99 61 1 22 120 3 100 1.44e-14 65.1
Msa0422490 AT4G15660.1 34.000 100 66 0 21 120 1 100 2.47e-14 64.3
Msa0422490 AT3G62950.1 30.693 101 69 1 21 120 1 101 3.11e-14 64.3
Msa0422490 AT1G28480.1 32.773 119 67 3 7 120 25 135 3.51e-14 65.1
Msa0422490 AT3G21460.1 33.000 100 67 0 21 120 1 100 1.23e-13 62.8
Msa0422490 AT2G47880.1 31.000 100 69 0 21 120 1 100 1.31e-13 62.4
Msa0422490 AT1G06830.1 31.915 94 64 0 21 114 1 94 3.43e-13 61.2
Msa0422490 AT3G62960.1 30.851 94 65 0 21 114 1 94 4.57e-13 61.2
Msa0422490 AT5G11930.1 33.645 107 61 3 15 120 49 146 6.27e-13 62.0
Msa0422490 AT2G47870.1 29.703 101 70 1 21 120 1 101 7.35e-13 60.5
Msa0422490 AT4G28730.2 40.789 76 41 2 16 90 68 140 1.46e-12 60.8
Msa0422490 AT2G30540.1 32.979 94 63 0 21 114 1 94 1.49e-12 59.7

Find 42 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATAGTTACAGAATGTTTA+TGG 0.206636 3_2:-71811540 Msa0422490:three_prime_UTR
CGTTGTTTCCATCTCTTCTT+TGG 0.212250 3_2:+71812650 None:intergenic
ACAGTACCTAATGTGTTTAT+TGG 0.265313 3_2:-71811799 Msa0422490:CDS
TTTATTTGTTGCAGCTGTTT+TGG 0.272729 3_2:-71811638 Msa0422490:intron
CAAATTCCATAACATAATAA+TGG 0.273756 3_2:-71812706 Msa0422490:exon
TCAGAGCTGAGCAGAGTTCT+TGG 0.303817 3_2:+71811561 None:intergenic
GTGAAAGAGGATCATCTTAT+TGG 0.307160 3_2:-71811378 Msa0422490:three_prime_UTR
TAGAACCCATTATTATGTTA+TGG 0.348332 3_2:+71812700 None:intergenic
CACTTTCTATATCCATTTCA+AGG 0.388516 3_2:+71812034 None:intergenic
ATCCATTTCAAGGACCTTGT+AGG 0.400003 3_2:+71812044 None:intergenic
CTTATTGTGGTTACTGCAAA+CGG 0.407824 3_2:-71812098 Msa0422490:CDS
ATAATAATGGGTTCTATGCT+TGG 0.419199 3_2:-71812693 Msa0422490:exon
ATTCCCCTTCTCACTGATGC+TGG 0.424957 3_2:-71811592 Msa0422490:CDS
AAATTCCATAACATAATAAT+GGG 0.425425 3_2:-71812705 Msa0422490:exon
TGTTATGGAATTTGATATGA+AGG 0.437446 3_2:+71812715 None:intergenic
AACCTACAAGGTCCTTGAAA+TGG 0.451012 3_2:-71812046 Msa0422490:CDS
TTGCCTCCAATAAACACATT+AGG 0.466203 3_2:+71811793 None:intergenic
AATCTTTAAATATAAAACTC+TGG 0.477736 3_2:-71811353 None:intergenic
CGCTCCAGCATCAGTGAGAA+GGG 0.479868 3_2:+71811588 None:intergenic
TATGTTAGTAGAGATATAGT+TGG 0.492609 3_2:-71811426 Msa0422490:three_prime_UTR
AATTCTAAAGTATGTGAAAG+AGG 0.492981 3_2:-71811391 Msa0422490:three_prime_UTR
TTATTGTGGTTACTGCAAAC+GGG 0.502636 3_2:-71812097 Msa0422490:CDS
AATAACCTGAAGACAAAGAC+GGG 0.504868 3_2:+71812594 None:intergenic
TGGTTACTGCAAACGGGTCA+AGG 0.522187 3_2:-71812091 Msa0422490:CDS
GTACCTAATGTGTTTATTGG+AGG 0.525018 3_2:-71811796 Msa0422490:CDS
ATTGGAGGCAAGCACATTGG+TGG 0.528449 3_2:-71811781 Msa0422490:CDS
TCAAGGGACCAAAGAAGAGA+TGG 0.547018 3_2:-71812658 Msa0422490:CDS
TCGCTCCAGCATCAGTGAGA+AGG 0.547446 3_2:+71811587 None:intergenic
CTTGGTTCTCAGAAGACTCA+AGG 0.550504 3_2:-71812675 Msa0422490:CDS
GCAGCATTAACTGAGTGGAC+AGG 0.622274 3_2:-71811829 Msa0422490:CDS
AAGGATCTACTCAAACAGCT+AGG 0.624288 3_2:-71812072 Msa0422490:CDS
AAATAACCTGAAGACAAAGA+CGG 0.625074 3_2:+71812593 None:intergenic
TTGGTTCTCAGAAGACTCAA+GGG 0.625272 3_2:-71812674 Msa0422490:CDS
TGTGACAGTAAGACTTATTG+TGG 0.631935 3_2:-71812111 Msa0422490:intron
ACAGCTAGGAGCAACCTACA+AGG 0.634355 3_2:-71812058 Msa0422490:CDS
TTTATTGGAGGCAAGCACAT+TGG 0.645273 3_2:-71811784 Msa0422490:CDS
TAACTGAGTGGACAGGACAA+AGG 0.651817 3_2:-71811822 Msa0422490:CDS
GGGGAATCAATTGACCTGCA+CGG 0.659613 3_2:+71811608 None:intergenic
GCTCCAGCATCAGTGAGAAG+GGG 0.673549 3_2:+71811589 None:intergenic
TTCATGCAGCATTAACTGAG+TGG 0.679113 3_2:-71811834 Msa0422490:CDS
ATAACCTGAAGACAAAGACG+GGG 0.734989 3_2:+71812595 None:intergenic
TAACCTGAAGACAAAGACGG+GGG 0.745087 3_2:+71812596 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTCCATAACATAATAAT+GGG - chr3_2:71811416-71811435 Msa0422490:three_prime_UTR 15.0%
!!! TCAATATTTTTTTAGGTAAT+AGG - chr3_2:71811611-71811630 Msa0422490:CDS 15.0%
!!! CAATATTTTTTTAGGTAATA+GGG - chr3_2:71811612-71811631 Msa0422490:CDS 15.0%
!!! AATATTTTTTTAGGTAATAG+GGG - chr3_2:71811613-71811632 Msa0422490:CDS 15.0%
!! AAATTCCATAACATAATAAT+GGG - chr3_2:71811416-71811435 Msa0422490:three_prime_UTR 15.0%
!!! TCAATATTTTTTTAGGTAAT+AGG - chr3_2:71811611-71811630 Msa0422490:CDS 15.0%
!!! CAATATTTTTTTAGGTAATA+GGG - chr3_2:71811612-71811631 Msa0422490:CDS 15.0%
!!! AATATTTTTTTAGGTAATAG+GGG - chr3_2:71811613-71811632 Msa0422490:CDS 15.0%
!! CAAATTCCATAACATAATAA+TGG - chr3_2:71811415-71811434 Msa0422490:three_prime_UTR 20.0%
!!! GAAATATGTCTTTTGATTTA+TGG - chr3_2:71811690-71811709 Msa0422490:intron 20.0%
!!! TGAGTTGAAAAATAAATTCA+AGG - chr3_2:71811800-71811819 Msa0422490:CDS 20.0%
!! ATTTCTCAATAAATACAAAG+TGG - chr3_2:71812195-71812214 Msa0422490:intron 20.0%
!! TTTCTCAATAAATACAAAGT+GGG - chr3_2:71812196-71812215 Msa0422490:intron 20.0%
!! CAAATTCCATAACATAATAA+TGG - chr3_2:71811415-71811434 Msa0422490:three_prime_UTR 20.0%
!!! GAAATATGTCTTTTGATTTA+TGG - chr3_2:71811690-71811709 Msa0422490:intron 20.0%
!!! TGAGTTGAAAAATAAATTCA+AGG - chr3_2:71811800-71811819 Msa0422490:CDS 20.0%
!! ATTTCTCAATAAATACAAAG+TGG - chr3_2:71812195-71812214 Msa0422490:intron 20.0%
!! TTTCTCAATAAATACAAAGT+GGG - chr3_2:71812196-71812215 Msa0422490:intron 20.0%
! TGTTATGGAATTTGATATGA+AGG + chr3_2:71811409-71811428 None:intergenic 25.0%
! TAGAACCCATTATTATGTTA+TGG + chr3_2:71811424-71811443 None:intergenic 25.0%
! GTTGATCAAAATACTAGTTA+AGG - chr3_2:71811583-71811602 Msa0422490:CDS 25.0%
!!! GGAGATGTCAATATTTTTTT+AGG - chr3_2:71811604-71811623 Msa0422490:CDS 25.0%
!!! TTATTTCTATATACTGTGCA+TGG - chr3_2:71811660-71811679 Msa0422490:intron 25.0%
!!! TTGTATTGAATTTGTTGTCA+AGG - chr3_2:71811899-71811918 Msa0422490:intron 25.0%
! TTTATCAAAATGAACAAGGA+GGG + chr3_2:71811949-71811968 None:intergenic 25.0%
! ATGCTTTATCAAAATGAACA+AGG + chr3_2:71811953-71811972 None:intergenic 25.0%
! TCACAACATAAATTACAGAT+GGG + chr3_2:71811995-71812014 None:intergenic 25.0%
! ATTTGTCTTTACCTATTTGA+AGG - chr3_2:71812250-71812269 Msa0422490:intron 25.0%
! GAATAGTTACAGAATGTTTA+TGG - chr3_2:71812581-71812600 Msa0422490:intron 25.0%
! TATGTTAGTAGAGATATAGT+TGG - chr3_2:71812695-71812714 Msa0422490:exon 25.0%
!!! AATTCTAAAGTATGTGAAAG+AGG - chr3_2:71812730-71812749 Msa0422490:five_prime_UTR 25.0%
! TGTTATGGAATTTGATATGA+AGG + chr3_2:71811409-71811428 None:intergenic 25.0%
! TAGAACCCATTATTATGTTA+TGG + chr3_2:71811424-71811443 None:intergenic 25.0%
! GTTGATCAAAATACTAGTTA+AGG - chr3_2:71811583-71811602 Msa0422490:CDS 25.0%
!!! GGAGATGTCAATATTTTTTT+AGG - chr3_2:71811604-71811623 Msa0422490:CDS 25.0%
!!! TTATTTCTATATACTGTGCA+TGG - chr3_2:71811660-71811679 Msa0422490:intron 25.0%
!!! TTGTATTGAATTTGTTGTCA+AGG - chr3_2:71811899-71811918 Msa0422490:intron 25.0%
! TTTATCAAAATGAACAAGGA+GGG + chr3_2:71811949-71811968 None:intergenic 25.0%
! ATGCTTTATCAAAATGAACA+AGG + chr3_2:71811953-71811972 None:intergenic 25.0%
! TCACAACATAAATTACAGAT+GGG + chr3_2:71811995-71812014 None:intergenic 25.0%
! ATTTGTCTTTACCTATTTGA+AGG - chr3_2:71812250-71812269 Msa0422490:intron 25.0%
! GAATAGTTACAGAATGTTTA+TGG - chr3_2:71812581-71812600 Msa0422490:intron 25.0%
! TATGTTAGTAGAGATATAGT+TGG - chr3_2:71812695-71812714 Msa0422490:exon 25.0%
!!! AATTCTAAAGTATGTGAAAG+AGG - chr3_2:71812730-71812749 Msa0422490:five_prime_UTR 25.0%
!! ATAATAATGGGTTCTATGCT+TGG - chr3_2:71811428-71811447 Msa0422490:three_prime_UTR 30.0%
AAATAACCTGAAGACAAAGA+CGG + chr3_2:71811531-71811550 None:intergenic 30.0%
! TCTAGCTTCAAATTAGAATG+AGG - chr3_2:71811853-71811872 Msa0422490:intron 30.0%
!! AATTTGTTGTCAAGGTAGTT+TGG - chr3_2:71811907-71811926 Msa0422490:intron 30.0%
CTTTATCAAAATGAACAAGG+AGG + chr3_2:71811950-71811969 None:intergenic 30.0%
GTCACAACATAAATTACAGA+TGG + chr3_2:71811996-71812015 None:intergenic 30.0%
CACTTTCTATATCCATTTCA+AGG + chr3_2:71812090-71812109 None:intergenic 30.0%
ACAGTACCTAATGTGTTTAT+TGG - chr3_2:71812322-71812341 Msa0422490:intron 30.0%
!! TTTATTTGTTGCAGCTGTTT+TGG - chr3_2:71812483-71812502 Msa0422490:intron 30.0%
!! ATAATAATGGGTTCTATGCT+TGG - chr3_2:71811428-71811447 Msa0422490:three_prime_UTR 30.0%
AAATAACCTGAAGACAAAGA+CGG + chr3_2:71811531-71811550 None:intergenic 30.0%
! TCTAGCTTCAAATTAGAATG+AGG - chr3_2:71811853-71811872 Msa0422490:intron 30.0%
!! AATTTGTTGTCAAGGTAGTT+TGG - chr3_2:71811907-71811926 Msa0422490:intron 30.0%
CTTTATCAAAATGAACAAGG+AGG + chr3_2:71811950-71811969 None:intergenic 30.0%
GTCACAACATAAATTACAGA+TGG + chr3_2:71811996-71812015 None:intergenic 30.0%
CACTTTCTATATCCATTTCA+AGG + chr3_2:71812090-71812109 None:intergenic 30.0%
ACAGTACCTAATGTGTTTAT+TGG - chr3_2:71812322-71812341 Msa0422490:intron 30.0%
!! TTTATTTGTTGCAGCTGTTT+TGG - chr3_2:71812483-71812502 Msa0422490:intron 30.0%
GGAGAAGACAAAAATTGAAG+AGG + chr3_2:71811388-71811407 None:intergenic 35.0%
AATAACCTGAAGACAAAGAC+GGG + chr3_2:71811530-71811549 None:intergenic 35.0%
TGACACTACAAAACCAAACA+CGG + chr3_2:71811722-71811741 None:intergenic 35.0%
AATTACAGATGGGATTGTCA+AGG + chr3_2:71811985-71812004 None:intergenic 35.0%
TGTGACAGTAAGACTTATTG+TGG - chr3_2:71812010-71812029 Msa0422490:intron 35.0%
CTTATTGTGGTTACTGCAAA+CGG - chr3_2:71812023-71812042 Msa0422490:intron 35.0%
TTATTGTGGTTACTGCAAAC+GGG - chr3_2:71812024-71812043 Msa0422490:intron 35.0%
! TGCTTTGTACCTTTTCTATC+TGG - chr3_2:71812127-71812146 Msa0422490:intron 35.0%
! GCTTTGTACCTTTTCTATCT+GGG - chr3_2:71812128-71812147 Msa0422490:intron 35.0%
AATCTCAACCCAGATAGAAA+AGG + chr3_2:71812139-71812158 None:intergenic 35.0%
CTTTACCTATTTGAAGGTGA+TGG - chr3_2:71812256-71812275 Msa0422490:intron 35.0%
GTACCTAATGTGTTTATTGG+AGG - chr3_2:71812325-71812344 Msa0422490:intron 35.0%
TTGCCTCCAATAAACACATT+AGG + chr3_2:71812331-71812350 None:intergenic 35.0%
CACATGAAAAAAGAAGCAAC+AGG + chr3_2:71812389-71812408 None:intergenic 35.0%
!!! CTGTTGCTTCTTTTTTCATG+TGG - chr3_2:71812387-71812406 Msa0422490:intron 35.0%
GTGAAAGAGGATCATCTTAT+TGG - chr3_2:71812743-71812762 Msa0422490:five_prime_UTR 35.0%
GGAGAAGACAAAAATTGAAG+AGG + chr3_2:71811388-71811407 None:intergenic 35.0%
AATAACCTGAAGACAAAGAC+GGG + chr3_2:71811530-71811549 None:intergenic 35.0%
TGACACTACAAAACCAAACA+CGG + chr3_2:71811722-71811741 None:intergenic 35.0%
AATTACAGATGGGATTGTCA+AGG + chr3_2:71811985-71812004 None:intergenic 35.0%
TGTGACAGTAAGACTTATTG+TGG - chr3_2:71812010-71812029 Msa0422490:intron 35.0%
CTTATTGTGGTTACTGCAAA+CGG - chr3_2:71812023-71812042 Msa0422490:intron 35.0%
TTATTGTGGTTACTGCAAAC+GGG - chr3_2:71812024-71812043 Msa0422490:intron 35.0%
! TGCTTTGTACCTTTTCTATC+TGG - chr3_2:71812127-71812146 Msa0422490:intron 35.0%
! GCTTTGTACCTTTTCTATCT+GGG - chr3_2:71812128-71812147 Msa0422490:intron 35.0%
AATCTCAACCCAGATAGAAA+AGG + chr3_2:71812139-71812158 None:intergenic 35.0%
CTTTACCTATTTGAAGGTGA+TGG - chr3_2:71812256-71812275 Msa0422490:intron 35.0%
GTACCTAATGTGTTTATTGG+AGG - chr3_2:71812325-71812344 Msa0422490:intron 35.0%
TTGCCTCCAATAAACACATT+AGG + chr3_2:71812331-71812350 None:intergenic 35.0%
CACATGAAAAAAGAAGCAAC+AGG + chr3_2:71812389-71812408 None:intergenic 35.0%
!!! CTGTTGCTTCTTTTTTCATG+TGG - chr3_2:71812387-71812406 Msa0422490:intron 35.0%
GTGAAAGAGGATCATCTTAT+TGG - chr3_2:71812743-71812762 Msa0422490:five_prime_UTR 35.0%
! TTGGTTCTCAGAAGACTCAA+GGG - chr3_2:71811447-71811466 Msa0422490:three_prime_UTR 40.0%
! CGTTGTTTCCATCTCTTCTT+TGG + chr3_2:71811474-71811493 None:intergenic 40.0%
ATAACCTGAAGACAAAGACG+GGG + chr3_2:71811529-71811548 None:intergenic 40.0%
GACACTACAAAACCAAACAC+GGG + chr3_2:71811721-71811740 None:intergenic 40.0%
AAGGATCTACTCAAACAGCT+AGG - chr3_2:71812049-71812068 Msa0422490:CDS 40.0%
AACCTACAAGGTCCTTGAAA+TGG - chr3_2:71812075-71812094 Msa0422490:CDS 40.0%
ATCCATTTCAAGGACCTTGT+AGG + chr3_2:71812080-71812099 None:intergenic 40.0%
CATCTCCATCACCTTCAAAT+AGG + chr3_2:71812264-71812283 None:intergenic 40.0%
TTCATGCAGCATTAACTGAG+TGG - chr3_2:71812287-71812306 Msa0422490:intron 40.0%
TTTATTGGAGGCAAGCACAT+TGG - chr3_2:71812337-71812356 Msa0422490:intron 40.0%
! TTGGTTCTCAGAAGACTCAA+GGG - chr3_2:71811447-71811466 Msa0422490:three_prime_UTR 40.0%
! CGTTGTTTCCATCTCTTCTT+TGG + chr3_2:71811474-71811493 None:intergenic 40.0%
ATAACCTGAAGACAAAGACG+GGG + chr3_2:71811529-71811548 None:intergenic 40.0%
GACACTACAAAACCAAACAC+GGG + chr3_2:71811721-71811740 None:intergenic 40.0%
AAGGATCTACTCAAACAGCT+AGG - chr3_2:71812049-71812068 Msa0422490:CDS 40.0%
AACCTACAAGGTCCTTGAAA+TGG - chr3_2:71812075-71812094 Msa0422490:CDS 40.0%
ATCCATTTCAAGGACCTTGT+AGG + chr3_2:71812080-71812099 None:intergenic 40.0%
CATCTCCATCACCTTCAAAT+AGG + chr3_2:71812264-71812283 None:intergenic 40.0%
TTCATGCAGCATTAACTGAG+TGG - chr3_2:71812287-71812306 Msa0422490:intron 40.0%
TTTATTGGAGGCAAGCACAT+TGG - chr3_2:71812337-71812356 Msa0422490:intron 40.0%
! CTTGGTTCTCAGAAGACTCA+AGG - chr3_2:71811446-71811465 Msa0422490:three_prime_UTR 45.0%
TCAAGGGACCAAAGAAGAGA+TGG - chr3_2:71811463-71811482 Msa0422490:three_prime_UTR 45.0%
TAACCTGAAGACAAAGACGG+GGG + chr3_2:71811528-71811547 None:intergenic 45.0%
TTTATGGTCAAGCCCGTGTT+TGG - chr3_2:71811706-71811725 Msa0422490:intron 45.0%
TAACTGAGTGGACAGGACAA+AGG - chr3_2:71812299-71812318 Msa0422490:intron 45.0%
! CATTGGTGGTTGTGACTGTA+AGG - chr3_2:71812354-71812373 Msa0422490:intron 45.0%
! CTTGGTTCTCAGAAGACTCA+AGG - chr3_2:71811446-71811465 Msa0422490:three_prime_UTR 45.0%
TCAAGGGACCAAAGAAGAGA+TGG - chr3_2:71811463-71811482 Msa0422490:three_prime_UTR 45.0%
TAACCTGAAGACAAAGACGG+GGG + chr3_2:71811528-71811547 None:intergenic 45.0%
TTTATGGTCAAGCCCGTGTT+TGG - chr3_2:71811706-71811725 Msa0422490:intron 45.0%
TAACTGAGTGGACAGGACAA+AGG - chr3_2:71812299-71812318 Msa0422490:intron 45.0%
! CATTGGTGGTTGTGACTGTA+AGG - chr3_2:71812354-71812373 Msa0422490:intron 45.0%
TGGTTACTGCAAACGGGTCA+AGG - chr3_2:71812030-71812049 Msa0422490:intron 50.0%
ACAGCTAGGAGCAACCTACA+AGG - chr3_2:71812063-71812082 Msa0422490:CDS 50.0%
GCAGCATTAACTGAGTGGAC+AGG - chr3_2:71812292-71812311 Msa0422490:intron 50.0%
ATTGGAGGCAAGCACATTGG+TGG - chr3_2:71812340-71812359 Msa0422490:intron 50.0%
!!! GTTTTGGAGAAACACCGTGC+AGG - chr3_2:71812499-71812518 Msa0422490:intron 50.0%
GGGGAATCAATTGACCTGCA+CGG + chr3_2:71812516-71812535 None:intergenic 50.0%
ATTCCCCTTCTCACTGATGC+TGG - chr3_2:71812529-71812548 Msa0422490:intron 50.0%
TCAGAGCTGAGCAGAGTTCT+TGG + chr3_2:71812563-71812582 None:intergenic 50.0%
TGGTTACTGCAAACGGGTCA+AGG - chr3_2:71812030-71812049 Msa0422490:intron 50.0%
ACAGCTAGGAGCAACCTACA+AGG - chr3_2:71812063-71812082 Msa0422490:CDS 50.0%
GCAGCATTAACTGAGTGGAC+AGG - chr3_2:71812292-71812311 Msa0422490:intron 50.0%
ATTGGAGGCAAGCACATTGG+TGG - chr3_2:71812340-71812359 Msa0422490:intron 50.0%
!!! GTTTTGGAGAAACACCGTGC+AGG - chr3_2:71812499-71812518 Msa0422490:intron 50.0%
GGGGAATCAATTGACCTGCA+CGG + chr3_2:71812516-71812535 None:intergenic 50.0%
ATTCCCCTTCTCACTGATGC+TGG - chr3_2:71812529-71812548 Msa0422490:intron 50.0%
TCAGAGCTGAGCAGAGTTCT+TGG + chr3_2:71812563-71812582 None:intergenic 50.0%
GAAGACAAAGACGGGGGAAG+AGG + chr3_2:71811522-71811541 None:intergenic 55.0%
CTTCCCCCGTCTTTGTCTTC+AGG - chr3_2:71811522-71811541 Msa0422490:three_prime_UTR 55.0%
GCTCCAGCATCAGTGAGAAG+GGG + chr3_2:71812535-71812554 None:intergenic 55.0%
CGCTCCAGCATCAGTGAGAA+GGG + chr3_2:71812536-71812555 None:intergenic 55.0%
TCGCTCCAGCATCAGTGAGA+AGG + chr3_2:71812537-71812556 None:intergenic 55.0%
GAAGACAAAGACGGGGGAAG+AGG + chr3_2:71811522-71811541 None:intergenic 55.0%
CTTCCCCCGTCTTTGTCTTC+AGG - chr3_2:71811522-71811541 Msa0422490:three_prime_UTR 55.0%
GCTCCAGCATCAGTGAGAAG+GGG + chr3_2:71812535-71812554 None:intergenic 55.0%
CGCTCCAGCATCAGTGAGAA+GGG + chr3_2:71812536-71812555 None:intergenic 55.0%
TCGCTCCAGCATCAGTGAGA+AGG + chr3_2:71812537-71812556 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 71811354 71812789 71811354 ID=Msa0422490;Name=Msa0422490
chr3_2 mRNA 71811354 71812789 71811354 ID=Msa0422490-mRNA-1;Parent=Msa0422490;Name=Msa0422490-mRNA-1;_AED=0.30;_eAED=0.30;_QI=80|1|1|1|1|1|4|207|127
chr3_2 exon 71811354 71811646 71811354 ID=Msa0422490-mRNA-1:exon:17610;Parent=Msa0422490-mRNA-1
chr3_2 exon 71811773 71811871 71811773 ID=Msa0422490-mRNA-1:exon:17609;Parent=Msa0422490-mRNA-1
chr3_2 exon 71812037 71812125 71812037 ID=Msa0422490-mRNA-1:exon:17608;Parent=Msa0422490-mRNA-1
chr3_2 exon 71812600 71812789 71812600 ID=Msa0422490-mRNA-1:exon:17607;Parent=Msa0422490-mRNA-1
chr3_2 five_prime_UTR 71812710 71812789 71812710 ID=Msa0422490-mRNA-1:five_prime_utr;Parent=Msa0422490-mRNA-1
chr3_2 CDS 71812600 71812709 71812600 ID=Msa0422490-mRNA-1:cds;Parent=Msa0422490-mRNA-1
chr3_2 CDS 71812037 71812125 71812037 ID=Msa0422490-mRNA-1:cds;Parent=Msa0422490-mRNA-1
chr3_2 CDS 71811773 71811871 71811773 ID=Msa0422490-mRNA-1:cds;Parent=Msa0422490-mRNA-1
chr3_2 CDS 71811561 71811646 71811561 ID=Msa0422490-mRNA-1:cds;Parent=Msa0422490-mRNA-1
chr3_2 three_prime_UTR 71811354 71811560 71811354 ID=Msa0422490-mRNA-1:three_prime_utr;Parent=Msa0422490-mRNA-1
Gene Sequence

>Msa0422490

ATGGGTTCTATGCTTGGTTCTCAGAAGACTCAAGGGACCAAAGAAGAGATGGAAACAACGTTGAATAAAACTAAACAGATTGCTGCCTCTTCCCCCGTCTTTGTCTTCAGTAAGACTTATTGTGGTTACTGCAAACGGGTCAAGGATCTACTCAAACAGCTAGGAGCAACCTACAAGGTCCTTGAAATGGATATAGAAAGTGATGGAGATGAGATTCATGCAGCATTAACTGAGTGGACAGGACAAAGGACAGTACCTAATGTGTTTATTGGAGGCAAGCACATTGGTGGTTGTGACTCTGTTTTGGAGAAACACCGTGCAGGTCAATTGATTCCCCTTCTCACTGATGCTGGAGCGATCGCCAAGAACTCTGCTCAGCTCTGA
Protein sequence

>Msa0422490

MGSMLGSQKTQGTKEEMETTLNKTKQIAASSPVFVFSKTYCGYCKRVKDLLKQLGATYKVLEMDIESDGDEIHAALTEWTGQRTVPNVFIGGKHIGGCDSVLEKHRAGQLIPLLTDAGAIAKNSAQL*