AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0423300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423300 MtrunA17_Chr3g0123971 98.544 206 3 0 1 206 1 206 2.03e-150 415
Msa0423300 MtrunA17_Chr8g0382221 94.686 207 10 1 1 206 1 207 5.16e-144 399
Msa0423300 MtrunA17_Chr5g0400131 84.541 207 31 1 1 206 1 207 1.14e-129 363
Msa0423300 MtrunA17_Chr4g0055081 85.507 207 28 2 1 206 1 206 3.18e-129 362
Msa0423300 MtrunA17_Chr1g0199331 47.561 164 85 1 9 171 31 194 3.79e-51 164
Msa0423300 MtrunA17_Chr5g0433561 44.099 161 90 0 11 171 12 172 1.54e-49 159
Msa0423300 MtrunA17_Chr3g0109721 43.478 161 91 0 11 171 12 172 2.92e-48 156
Msa0423300 MtrunA17_Chr8g0390561 44.375 160 89 0 11 170 11 170 5.44e-47 153
Msa0423300 MtrunA17_Chr8g0349711 40.341 176 103 1 10 185 7 180 5.23e-46 151
Msa0423300 MtrunA17_Chr4g0032201 41.401 157 92 0 10 166 13 169 2.33e-45 149
Msa0423300 MtrunA17_Chr8g0349681 40.909 176 102 1 10 185 176 349 5.11e-44 154
Msa0423300 MtrunA17_Chr8g0349681 42.197 173 94 1 10 182 385 551 7.19e-41 145
Msa0423300 MtrunA17_Chr8g0349681 40.123 162 97 0 10 171 14 175 2.98e-40 144
Msa0423300 MtrunA17_Chr6g0458081 35.533 197 119 2 10 203 7 198 6.72e-44 145
Msa0423300 MtrunA17_Chr8g0351861 37.888 161 100 0 10 170 17 177 8.97e-44 146
Msa0423300 MtrunA17_Chr2g0276931 43.478 161 91 0 10 170 10 170 1.12e-43 145
Msa0423300 MtrunA17_Chr2g0276941 37.158 183 112 1 10 189 16 198 1.93e-43 145
Msa0423300 MtrunA17_Chr8g0346421 42.038 157 91 0 10 166 27 183 4.33e-43 144
Msa0423300 MtrunA17_Chr3g0123821 34.518 197 114 1 10 206 17 198 6.84e-43 143
Msa0423300 MtrunA17_Chr7g0234201 41.558 154 90 0 10 163 14 167 1.10e-42 142
Msa0423300 MtrunA17_Chr2g0290891 37.267 161 101 0 10 170 7 167 1.39e-42 142
Msa0423300 MtrunA17_Chr3g0112681 37.640 178 102 1 10 178 13 190 3.41e-42 141
Msa0423300 MtrunA17_Chr7g0231131 34.896 192 112 1 10 201 16 194 3.42e-42 141
Msa0423300 MtrunA17_Chr8g0381841 34.010 197 115 1 10 206 18 199 8.54e-42 140
Msa0423300 MtrunA17_Chr7g0250531 42.236 161 93 0 10 170 9 169 1.19e-41 139
Msa0423300 MtrunA17_Chr7g0214241 35.961 203 124 2 10 206 16 218 1.53e-41 140
Msa0423300 MtrunA17_Chr4g0055651 41.615 161 94 0 10 170 9 169 1.66e-41 139
Msa0423300 MtrunA17_Chr6g0482721 37.356 174 109 0 10 183 13 186 2.14e-41 139
Msa0423300 MtrunA17_Chr1g0174281 35.266 207 122 3 10 204 16 222 2.51e-41 139
Msa0423300 MtrunA17_Chr7g0275991 38.182 165 98 1 6 166 13 177 4.20e-41 139
Msa0423300 MtrunA17_Chr8g0335471 40.994 161 95 0 10 170 9 169 1.84e-40 136
Msa0423300 MtrunA17_Chr1g0189361 38.217 157 97 0 10 166 15 171 2.60e-40 137
Msa0423300 MtrunA17_Chr4g0030911 36.548 197 122 2 11 204 17 213 4.84e-40 136
Msa0423300 MtrunA17_Chr4g0046261 36.306 157 100 0 10 166 14 170 8.54e-40 135
Msa0423300 MtrunA17_Chr2g0315551 35.669 157 101 0 10 166 14 170 1.18e-39 135
Msa0423300 MtrunA17_Chr1g0212521 36.364 198 122 3 11 204 17 214 1.37e-39 135
Msa0423300 MtrunA17_Chr1g0182321 42.405 158 90 1 11 167 17 174 3.00e-39 134
Msa0423300 MtrunA17_Chr2g0327911 37.056 197 120 3 11 204 17 212 3.25e-39 134
Msa0423300 MtrunA17_Chr1g0182301 42.405 158 90 1 11 167 17 174 4.82e-39 133
Msa0423300 MtrunA17_Chr4g0040781 36.306 157 100 0 10 166 14 170 4.85e-39 133
Msa0423300 MtrunA17_Chr7g0263071 42.405 158 90 1 11 167 17 174 5.09e-39 133
Msa0423300 MtrunA17_Chr2g0283131 36.306 157 100 0 10 166 14 170 8.49e-39 132
Msa0423300 MtrunA17_Chr2g0319951 34.783 161 105 0 10 170 14 174 1.88e-38 132
Msa0423300 MtrunA17_Chr2g0325091 33.663 202 128 2 10 205 12 213 2.44e-38 131
Msa0423300 MtrunA17_Chr4g0004661 39.216 204 110 5 11 206 66 263 2.82e-38 132
Msa0423300 MtrunA17_Chr4g0034871 38.235 204 111 4 11 206 10 206 4.48e-38 130
Msa0423300 MtrunA17_Chr2g0331691 36.946 203 116 3 11 206 12 209 1.79e-37 129
Msa0423300 MtrunA17_Chr8g0383261 33.498 203 121 4 11 206 14 209 5.45e-36 125
Msa0423300 MtrunA17_Chr1g0197331 37.186 199 120 4 10 205 16 212 1.64e-35 124
Msa0423300 MtrunA17_Chr8g0384311 41.509 159 90 3 10 166 14 171 2.02e-35 124
Msa0423300 MtrunA17_Chr1g0201121 34.975 203 120 4 11 206 10 207 2.31e-35 124
Msa0423300 MtrunA17_Chr6g0466301 35.294 204 117 5 11 206 10 206 2.61e-35 123
Msa0423300 MtrunA17_Chr6g0465491 35.294 204 117 5 11 206 10 206 2.61e-35 123
Msa0423300 MtrunA17_Chr1g0180291 36.041 197 123 3 10 204 15 210 2.89e-35 123
Msa0423300 MtrunA17_Chr8g0392931 37.267 161 101 0 10 170 15 175 3.58e-35 123
Msa0423300 MtrunA17_Chr7g0265001 33.333 198 130 2 10 205 17 214 7.68e-34 120
Msa0423300 MtrunA17_Chr3g0130271 39.412 170 99 4 10 176 13 181 1.65e-32 116
Msa0423300 MtrunA17_Chr1g0159481 37.278 169 103 3 10 176 18 185 5.22e-32 115
Msa0423300 MtrunA17_Chr3g0138121 32.143 168 111 3 10 177 14 178 1.02e-24 96.7
Msa0423300 MtrunA17_Chr3g0138111 32.143 168 111 3 10 177 14 178 1.04e-24 96.7
Msa0423300 MtrunA17_Chr3g0138101 32.143 168 111 3 10 177 14 178 1.05e-24 96.7
Msa0423300 MtrunA17_Chr3g0117931 35.758 165 92 5 11 163 10 172 6.69e-22 89.0
Msa0423300 MtrunA17_Chr2g0325101 35.152 165 93 5 11 163 8 170 1.35e-21 87.8
Msa0423300 MtrunA17_Chr6g0486041 34.545 165 94 5 11 163 8 170 3.62e-20 84.3
Msa0423300 MtrunA17_Chr6g0466161 28.191 188 121 5 26 206 7 187 9.60e-20 83.2
Msa0423300 MtrunA17_Chr4g0046211 36.154 130 80 3 11 139 8 135 2.45e-19 80.5
Msa0423300 MtrunA17_Chr8g0371501 30.909 165 100 4 11 163 8 170 4.51e-19 81.3
Msa0423300 MtrunA17_Chr6g0465471 27.660 188 122 5 26 206 7 187 4.76e-19 81.3
Msa0423300 MtrunA17_Chr5g0405791 30.909 165 100 4 11 163 8 170 5.34e-19 80.9
Msa0423300 MtrunA17_Chr4g0037021 31.515 165 99 5 11 163 8 170 1.09e-18 80.1
Msa0423300 MtrunA17_Chr6g0466431 38.542 96 50 1 11 106 10 96 4.95e-18 75.9
Msa0423300 MtrunA17_Chr6g0465611 38.542 96 50 1 11 106 10 96 4.95e-18 75.9
Msa0423300 MtrunA17_Chr5g0443631 25.146 171 122 3 5 170 100 269 1.30e-13 67.8
Msa0423300 MtrunA17_Chr1g0178291 32.051 156 97 4 12 166 431 578 4.42e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423300 AT5G10260.1 88.406 207 23 1 1 206 1 207 1.09e-136 381
Msa0423300 AT2G22290.1 88.889 207 22 1 1 206 1 207 1.89e-135 378
Msa0423300 AT2G44610.1 86.058 208 27 2 1 206 1 208 4.13e-131 367
Msa0423300 AT4G39890.1 78.972 214 37 3 1 206 1 214 2.58e-117 332
Msa0423300 AT5G64990.1 78.392 199 41 2 10 206 8 206 3.23e-108 309
Msa0423300 AT5G64990.2 75.728 206 41 3 10 206 8 213 2.37e-105 302
Msa0423300 AT4G19640.1 44.720 161 89 0 11 171 12 172 1.12e-50 163
Msa0423300 AT3G54840.1 47.561 164 85 1 9 171 33 196 1.43e-50 162
Msa0423300 AT5G45130.1 44.720 161 89 0 11 171 12 172 1.45e-50 162
Msa0423300 AT3G54840.3 47.826 161 83 1 12 171 2 162 5.73e-50 160
Msa0423300 AT5G45130.2 44.025 159 89 0 13 171 22 180 1.18e-48 158
Msa0423300 AT3G54840.2 48.077 156 80 1 9 163 33 188 1.90e-48 157
Msa0423300 AT1G09630.1 40.252 159 95 0 10 168 13 171 9.24e-44 145
Msa0423300 AT3G11730.1 38.889 198 114 2 10 204 9 202 1.23e-43 145
Msa0423300 AT5G65270.1 38.217 157 97 0 10 166 18 174 1.41e-43 145
Msa0423300 AT1G07410.1 39.752 161 97 0 10 170 13 173 1.61e-43 145
Msa0423300 AT4G17160.1 38.272 162 100 0 9 170 6 167 2.54e-43 144
Msa0423300 AT1G05810.1 37.255 204 119 3 10 206 13 214 2.54e-43 145
Msa0423300 AT4G35860.1 34.615 208 118 3 10 204 7 209 3.40e-43 144
Msa0423300 AT1G05810.2 37.255 204 119 3 10 206 59 260 6.75e-43 145
Msa0423300 AT4G39990.1 36.646 161 102 0 10 170 18 178 1.28e-42 143
Msa0423300 AT4G17170.1 37.267 161 101 0 10 170 7 167 1.65e-42 142
Msa0423300 AT3G12160.1 36.946 203 120 3 10 204 16 218 1.85e-42 142
Msa0423300 AT5G59150.1 39.490 157 95 0 10 166 13 169 2.04e-42 142
Msa0423300 AT2G31680.1 35.122 205 123 3 10 206 13 215 4.25e-42 142
Msa0423300 AT3G46830.1 38.854 157 96 0 10 166 13 169 4.78e-42 141
Msa0423300 AT3G46830.2 38.854 157 96 0 10 166 13 169 4.78e-42 141
Msa0423300 AT3G07410.1 42.208 154 89 0 10 163 13 166 8.04e-42 141
Msa0423300 AT5G47960.1 34.804 204 124 2 10 204 16 219 1.45e-41 140
Msa0423300 AT1G18200.1 40.909 154 91 0 10 163 14 167 2.09e-41 140
Msa0423300 AT1G02130.1 40.994 161 95 0 10 170 9 169 3.56e-41 139
Msa0423300 AT5G47520.1 38.217 157 97 0 10 166 15 171 8.39e-41 138
Msa0423300 AT2G43130.1 38.217 157 97 0 10 166 13 169 1.05e-40 138
Msa0423300 AT3G15060.1 36.646 161 102 0 10 170 14 174 2.24e-40 137
Msa0423300 AT4G17530.1 40.373 161 96 0 10 170 9 169 4.03e-40 136
Msa0423300 AT5G47200.1 40.373 161 96 0 10 170 9 169 4.49e-40 136
Msa0423300 AT4G18430.1 33.010 206 125 3 10 205 14 216 1.10e-39 135
Msa0423300 AT5G60860.1 36.025 161 103 0 10 170 14 174 1.13e-39 135
Msa0423300 AT3G18820.1 38.725 204 110 4 11 206 10 206 1.17e-39 135
Msa0423300 AT1G73640.1 38.961 154 94 0 10 163 14 167 1.74e-39 135
Msa0423300 AT4G19640.2 45.238 126 69 0 11 136 12 137 3.56e-39 132
Msa0423300 AT1G28550.1 37.580 157 98 0 10 166 14 170 5.91e-39 134
Msa0423300 AT5G59840.1 37.056 197 111 3 11 203 17 204 7.74e-39 133
Msa0423300 AT1G16920.1 35.032 157 102 0 10 166 14 170 8.71e-39 133
Msa0423300 AT3G46060.2 41.772 158 91 1 11 167 17 174 2.38e-38 132
Msa0423300 AT3G46060.3 41.772 158 91 1 11 167 17 174 2.38e-38 132
Msa0423300 AT3G46060.1 41.772 158 91 1 11 167 17 174 2.38e-38 132
Msa0423300 AT1G06400.1 36.306 157 100 0 10 166 14 170 2.85e-38 132
Msa0423300 AT1G01200.1 36.190 210 120 5 10 206 29 237 2.87e-38 132
Msa0423300 AT4G19640.3 45.600 125 68 0 11 135 12 136 4.86e-38 130
Msa0423300 AT1G52280.1 38.235 204 111 4 11 206 10 206 6.19e-38 130
Msa0423300 AT3G53610.2 41.772 158 91 1 11 167 17 174 7.05e-38 130
Msa0423300 AT3G53610.3 41.772 158 91 1 11 167 17 174 7.05e-38 130
Msa0423300 AT3G53610.1 41.772 158 91 1 11 167 17 174 7.05e-38 130
Msa0423300 AT5G03520.1 38.889 198 118 3 11 205 17 214 1.03e-37 130
Msa0423300 AT3G16100.1 38.725 204 110 4 11 206 10 206 3.75e-37 129
Msa0423300 AT5G45750.1 35.220 159 99 2 10 166 14 170 3.75e-37 129
Msa0423300 AT2G33870.1 36.306 157 100 0 10 166 14 170 7.30e-37 128
Msa0423300 AT2G21880.1 35.714 210 113 5 11 206 11 212 2.34e-36 127
Msa0423300 AT4G18800.1 34.591 159 100 2 10 166 14 170 5.96e-36 125
Msa0423300 AT1G49300.2 36.275 204 115 4 11 206 10 206 2.27e-35 124
Msa0423300 AT1G49300.1 36.275 204 115 4 11 206 10 206 2.27e-35 124
Msa0423300 AT3G09900.1 43.038 158 89 1 11 167 17 174 4.74e-35 123
Msa0423300 AT4G09720.1 36.275 204 115 7 11 206 10 206 1.15e-34 122
Msa0423300 AT1G22740.1 35.468 203 115 5 11 206 10 203 2.92e-34 121
Msa0423300 AT5G03530.2 34.848 198 126 3 10 205 14 210 8.77e-34 120
Msa0423300 AT5G03530.1 34.848 198 126 3 10 205 14 210 8.77e-34 120
Msa0423300 AT4G09720.4 35.407 209 115 7 11 206 10 211 2.72e-33 119
Msa0423300 AT1G43890.3 37.791 172 104 3 10 179 14 184 3.04e-33 119
Msa0423300 AT1G43890.1 37.791 172 104 3 10 179 14 184 3.04e-33 119
Msa0423300 AT1G43890.2 37.791 172 104 3 10 179 14 184 3.04e-33 119
Msa0423300 AT4G09720.3 34.419 215 115 7 11 206 10 217 1.20e-32 117
Msa0423300 AT4G35860.2 36.364 165 87 3 53 204 4 163 1.61e-32 115
Msa0423300 AT5G39620.1 35.922 206 118 6 9 206 5 204 4.12e-32 115
Msa0423300 AT5G03520.2 37.576 165 100 3 44 205 40 204 4.63e-30 110
Msa0423300 AT3G09910.3 36.869 198 116 5 10 205 14 204 6.64e-30 110
Msa0423300 AT3G09910.1 36.869 198 116 5 10 205 14 204 6.64e-30 110
Msa0423300 AT3G09910.2 36.869 198 116 5 10 205 14 204 6.64e-30 110
Msa0423300 AT2G21880.4 33.846 195 107 5 26 206 1 187 2.07e-29 108
Msa0423300 AT2G21880.3 33.846 195 107 5 26 206 1 187 2.07e-29 108
Msa0423300 AT3G09910.5 38.750 160 95 3 10 167 14 172 3.54e-29 107
Msa0423300 AT3G09910.4 38.750 160 95 3 10 167 14 172 3.54e-29 107
Msa0423300 AT5G59150.2 39.474 114 69 0 53 166 5 118 6.81e-28 103
Msa0423300 AT2G21880.2 32.381 210 112 6 11 206 11 204 6.22e-27 102
Msa0423300 AT4G09720.6 35.593 177 99 7 38 206 3 172 1.85e-25 97.8
Msa0423300 AT4G09720.5 35.593 177 99 7 38 206 3 172 1.85e-25 97.8
Msa0423300 AT4G09720.2 35.593 177 99 7 38 206 3 172 1.85e-25 97.8
Msa0423300 AT5G55080.1 30.409 171 116 3 7 177 11 178 2.09e-25 99.0
Msa0423300 AT5G20020.1 32.143 168 111 3 10 177 14 178 5.72e-25 97.8
Msa0423300 AT5G20010.1 32.143 168 111 3 10 177 14 178 7.86e-25 97.4
Msa0423300 AT5G55190.1 32.143 168 111 3 10 177 14 178 1.72e-24 96.3
Msa0423300 AT2G44690.1 35.152 165 93 5 11 163 20 182 9.66e-23 91.7
Msa0423300 AT3G48040.1 35.758 165 92 5 11 163 10 172 4.52e-21 87.0
Msa0423300 AT4G35020.1 33.333 165 96 5 11 163 8 170 1.68e-20 85.5
Msa0423300 AT4G35020.2 33.333 165 96 5 11 163 8 170 1.68e-20 85.5
Msa0423300 AT4G35020.3 33.333 165 96 5 11 163 8 170 1.68e-20 85.5
Msa0423300 AT1G20090.1 32.727 165 97 4 11 163 7 169 6.67e-20 83.6
Msa0423300 AT1G75840.1 32.727 165 97 4 11 163 8 170 7.18e-20 83.6
Msa0423300 AT5G62880.1 34.545 165 94 5 11 163 10 172 7.47e-20 84.0
Msa0423300 AT3G51300.1 32.121 165 98 4 11 163 8 170 2.03e-19 82.4
Msa0423300 AT4G35950.1 32.727 165 97 4 11 163 8 170 2.05e-19 82.4
Msa0423300 AT2G17800.2 32.727 165 97 4 11 163 8 170 2.67e-19 82.0
Msa0423300 AT2G17800.1 32.727 165 97 4 11 163 8 170 2.67e-19 82.0
Msa0423300 AT3G48040.2 35.897 156 86 5 11 154 10 163 3.59e-19 81.3
Msa0423300 AT4G28950.1 32.515 163 98 5 11 163 8 168 1.79e-18 80.1
Msa0423300 AT5G62880.2 38.525 122 72 3 11 131 10 129 3.70e-18 79.0
Msa0423300 AT5G45970.1 30.909 165 100 5 11 163 8 170 4.12e-18 79.3
Msa0423300 AT4G35020.4 35.965 114 70 3 11 123 8 119 2.06e-15 70.5
Msa0423300 AT4G35020.5 35.965 114 70 3 11 123 8 119 2.06e-15 70.5
Msa0423300 AT5G46025.1 36.782 87 54 1 85 170 20 106 4.15e-15 68.9
Msa0423300 AT3G51290.2 31.818 132 77 4 44 163 641 771 2.46e-11 62.8

Find 55 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCACTTGACACAATTAT+TGG 0.192149 3_2:+73057958 Msa0423300:three_prime_UTR
TATTGGCTTGGCAACTAAAA+TGG 0.194288 3_2:+73057975 Msa0423300:three_prime_UTR
TAAGAAAACGAGTTTGTATT+TGG 0.198916 3_2:-73053786 None:intergenic
GAACGTGAATAACGCGAATC+TGG 0.310056 3_2:-73053706 None:intergenic
CAGTGATGTTATTATCGTTT+TGG 0.337333 3_2:+73056637 Msa0423300:CDS
TCCACCTTGCTGCTCTGTCC+TGG 0.375622 3_2:-73057891 None:intergenic
CATTGGTACAAAAGTACGTT+TGG 0.379590 3_2:-73058019 None:intergenic
GAAGCAATCTTGCGAAATAA+AGG 0.385222 3_2:-73057788 None:intergenic
CAATGCTCTTCAGTTTAGTT+TGG 0.388867 3_2:+73058037 Msa0423300:three_prime_UTR
AAGATTGCTTCAGCCTTGCC+CGG 0.405262 3_2:+73057800 Msa0423300:CDS
TACAAAAGTACGTTTGGGTT+TGG 0.416717 3_2:-73058013 None:intergenic
GAACAGTTCGTTTGCAGCTC+TGG 0.419041 3_2:+73054826 Msa0423300:CDS
TAAATTCGACACTACCTATC+AGG 0.424038 3_2:+73053859 Msa0423300:CDS
CTCTGTCCTGGATGAATTCA+CGG 0.424353 3_2:-73057879 None:intergenic
TAGTGTTAAGAAATGATTGC+CGG 0.427909 3_2:-73056580 None:intergenic
AGTACGTTTGGGTTTGGCCT+AGG 0.430088 3_2:-73058007 None:intergenic
AACGAACTGTTCGATCTTCA+AGG 0.432629 3_2:-73054815 None:intergenic
TCTACATTTCAGGCGACAAT+TGG 0.437446 3_2:+73054765 Msa0423300:intron
ACTTGACACAATTATTGGCT+TGG 0.447901 3_2:+73057963 Msa0423300:three_prime_UTR
GAAAAGTGGAATAACCTGAT+AGG 0.460045 3_2:-73053873 None:intergenic
GCTCTTCAGTTTAGTTTGGT+AGG 0.461266 3_2:+73058041 Msa0423300:three_prime_UTR
TGCTTCAGCCTTGCCCGGGA+TGG 0.464859 3_2:+73057805 Msa0423300:CDS
ATGCCAAGTCGCGTGAGAGC+GGG 0.477457 3_2:+73057438 Msa0423300:CDS
AACGTGAATAACGCGAATCT+GGG 0.495484 3_2:-73053705 None:intergenic
ATTGGTACAAAAGTACGTTT+GGG 0.496039 3_2:-73058018 None:intergenic
TTCTTAGGTGATCAATCCGT+TGG 0.500123 3_2:+73053803 Msa0423300:CDS
GAAGAATCTCTTATATAACT+TGG 0.525048 3_2:-73054988 None:intergenic
TAACACTAACAAGTGGGTTG+AGG 0.526084 3_2:+73056595 Msa0423300:CDS
AGGACAGAGCAGCAAGGTGG+AGG 0.526096 3_2:+73057893 Msa0423300:CDS
AGATTGCTTCAGCCTTGCCC+GGG 0.531466 3_2:+73057801 Msa0423300:CDS
ATAGAAACCAGTGCAAAAGC+AGG 0.533135 3_2:+73057470 Msa0423300:CDS
GTTGAAAGACTTTCCATCCC+GGG 0.541408 3_2:-73057818 None:intergenic
TAACTCCACAGGGATACTGC+AGG 0.545708 3_2:+73054940 Msa0423300:intron
TAATAGGCAAGTCTCTATAG+AGG 0.546908 3_2:+73057409 Msa0423300:intron
AAGACTTTCCATCCCGGGCA+AGG 0.551250 3_2:-73057813 None:intergenic
GATCCCGCTCTCACGCGACT+TGG 0.552486 3_2:-73057441 None:intergenic
CACTAACAAGTGGGTTGAGG+AGG 0.555833 3_2:+73056598 Msa0423300:CDS
ACGAGTTTGTATTTGGCTAG+AGG 0.566889 3_2:-73053779 None:intergenic
TGTTGAAAGACTTTCCATCC+CGG 0.567013 3_2:-73057819 None:intergenic
CAAACTAAACTGAAGAGCAT+TGG 0.570927 3_2:-73058036 None:intergenic
AAAAGCAGGATTTAATATCA+AGG 0.572165 3_2:+73057484 Msa0423300:CDS
ATTTCTTAACACTAACAAGT+GGG 0.572336 3_2:+73056589 Msa0423300:CDS
TTCAACAAAGCAAGAAGACA+TGG 0.574137 3_2:+73057835 Msa0423300:CDS
AATCTACCGTGAATTCATCC+AGG 0.579097 3_2:+73057873 Msa0423300:CDS
CATTTCTTAACACTAACAAG+TGG 0.582798 3_2:+73056588 Msa0423300:CDS
AGGCAAGTCTCTATAGAGGA+AGG 0.591101 3_2:+73057413 Msa0423300:intron
GATGCCAAGTCGCGTGAGAG+CGG 0.614649 3_2:+73057437 Msa0423300:CDS
AACTCCACAGGGATACTGCA+GGG 0.615234 3_2:+73054941 Msa0423300:intron
TAAAATGGAAATATGCACCT+AGG 0.622590 3_2:+73057990 Msa0423300:three_prime_UTR
TATAAGAGATTCTTCAGTCG+CGG 0.624663 3_2:+73054996 Msa0423300:CDS
TCCAGGACAGAGCAGCAAGG+TGG 0.660114 3_2:+73057890 Msa0423300:CDS
CATGAAGCGAGTGATGATGC+TGG 0.674864 3_2:-73053831 None:intergenic
GAGGAGGTACGTGCAGAACG+AGG 0.683803 3_2:+73056614 Msa0423300:CDS
TCATCCAGGACAGAGCAGCA+AGG 0.690459 3_2:+73057887 Msa0423300:CDS
AAACAGTGCTATTCACAACA+AGG 0.704518 3_2:+73057927 Msa0423300:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAGAAACAAAAA+TGG + chr3_2:73053745-73053764 Msa0423300:exon 10.0%
!! TAATTATTATACATTCTTTA+AGG + chr3_2:73056337-73056356 Msa0423300:intron 10.0%
!! AGAAATTGAAAAAAATGAAA+CGG - chr3_2:73053942-73053961 None:intergenic 15.0%
!! ATTCAAAATATGCAAAAAAT+GGG - chr3_2:73054909-73054928 None:intergenic 15.0%
!!! TAATTTTTTTTCAATCTATG+AGG + chr3_2:73056505-73056524 Msa0423300:intron 15.0%
!! AATAAGTTAGAAATGAAAAG+TGG - chr3_2:73053890-73053909 None:intergenic 20.0%
!! CAATAAAACATTCACTAAAT+AGG - chr3_2:73054107-73054126 None:intergenic 20.0%
!! TTTATTGTGTATTAAGAACA+AGG + chr3_2:73054120-73054139 Msa0423300:intron 20.0%
!!! AATTGTAGGAATTATTTTCA+AGG + chr3_2:73054481-73054500 Msa0423300:intron 20.0%
!!! ATTGTAGGAATTATTTTCAA+GGG + chr3_2:73054482-73054501 Msa0423300:intron 20.0%
!! CATTCAAAATATGCAAAAAA+TGG - chr3_2:73054910-73054929 None:intergenic 20.0%
!! CAAAAAAAGTTAATACTTAC+TGG - chr3_2:73055040-73055059 None:intergenic 20.0%
!! TTTAACGTTAGCAAATAATT+TGG - chr3_2:73055203-73055222 None:intergenic 20.0%
!!! TCATGAACTTATTTTAACTT+TGG - chr3_2:73055554-73055573 None:intergenic 20.0%
!!! CATTGTAATTTGTAAGTTTA+TGG - chr3_2:73055919-73055938 None:intergenic 20.0%
!! TTACAAATTACAATGTTGTA+TGG + chr3_2:73055924-73055943 Msa0423300:intron 20.0%
!! TACAAATTACAATGTTGTAT+GGG + chr3_2:73055925-73055944 Msa0423300:intron 20.0%
!!! TGTTTCGTTTTCAAAAAAAT+TGG + chr3_2:73056850-73056869 Msa0423300:intron 20.0%
!! GTTCTGTTTGAATATTATAA+TGG + chr3_2:73056896-73056915 Msa0423300:intron 20.0%
!! TGTTTGAATATTATAATGGT+AGG + chr3_2:73056900-73056919 Msa0423300:intron 20.0%
!! TATTTCAAAACCATATTTAG+AGG + chr3_2:73057074-73057093 Msa0423300:intron 20.0%
!! TATGAATTTACCTCTAAATA+TGG - chr3_2:73057087-73057106 None:intergenic 20.0%
!! ATATTTAGAGGTAAATTCAT+AGG + chr3_2:73057086-73057105 Msa0423300:intron 20.0%
!! GAAAAAAACAAAATATGATG+AGG - chr3_2:73057305-73057324 None:intergenic 20.0%
!!! ATTTTGTTTTTTTCCTCATA+TGG + chr3_2:73057311-73057330 Msa0423300:intron 20.0%
! TAAGAAAACGAGTTTGTATT+TGG - chr3_2:73053789-73053808 None:intergenic 25.0%
!! AAATACAAACTCGTTTTCTT+AGG + chr3_2:73053788-73053807 Msa0423300:CDS 25.0%
!! ATTTTATTCTGCATTGTCTT+TGG + chr3_2:73054287-73054306 Msa0423300:intron 25.0%
! TAAGCAAAATTGTTTCGATT+AGG + chr3_2:73054350-73054369 Msa0423300:intron 25.0%
!!! TTGTAGGAATTATTTTCAAG+GGG + chr3_2:73054483-73054502 Msa0423300:intron 25.0%
! GAAGAATCTCTTATATAACT+TGG - chr3_2:73054991-73055010 None:intergenic 25.0%
! CAAAAAAAGTTAATACTGAC+TGG - chr3_2:73055106-73055125 None:intergenic 25.0%
! AAGAAAACAATACAATAGCA+TGG + chr3_2:73055133-73055152 Msa0423300:intron 25.0%
!!! GTCTTACATTAAAAGTACTT+CGG + chr3_2:73055643-73055662 Msa0423300:intron 25.0%
! AAACTAAATGACCGATATAT+AGG + chr3_2:73055782-73055801 Msa0423300:intron 25.0%
!! AAGCTTAAACGAAGATTTTA+CGG + chr3_2:73055888-73055907 Msa0423300:intron 25.0%
! AGGAACAAAAATACATTTCT+TGG + chr3_2:73056044-73056063 Msa0423300:intron 25.0%
! TGATATCATAATTTCTGTCA+TGG - chr3_2:73056209-73056228 None:intergenic 25.0%
! ATTATGATATCATACGATCT+AGG + chr3_2:73056217-73056236 Msa0423300:intron 25.0%
! ATTTCTTAACACTAACAAGT+GGG + chr3_2:73056589-73056608 Msa0423300:CDS 25.0%
! AAACTGATCTTGTTGAAAAA+AGG + chr3_2:73056669-73056688 Msa0423300:CDS 25.0%
! GAATTATAGCAAACATAGTA+TGG - chr3_2:73056829-73056848 None:intergenic 25.0%
! GTAATACTAAAAATTCGTAC+AGG + chr3_2:73057021-73057040 Msa0423300:intron 25.0%
! GAAATAACAACTTCAAAGTA+TGG - chr3_2:73057060-73057079 None:intergenic 25.0%
! TATATCCAACAATATCATCA+TGG - chr3_2:73057113-73057132 None:intergenic 25.0%
! ATTGTTGGATATAATGTTTC+CGG + chr3_2:73057120-73057139 Msa0423300:intron 25.0%
! TTGCTATAAGAAGAATTGTA+TGG - chr3_2:73057344-73057363 None:intergenic 25.0%
! AAGCATCATAATGTCATTAT+AGG - chr3_2:73057389-73057408 None:intergenic 25.0%
! TGACATTATGATGCTTTAAT+AGG + chr3_2:73057393-73057412 Msa0423300:intron 25.0%
! AAAAGCAGGATTTAATATCA+AGG + chr3_2:73057484-73057503 Msa0423300:CDS 25.0%
! TACATCATACATGAACATAT+GGG - chr3_2:73057537-73057556 None:intergenic 25.0%
! ATACATCATACATGAACATA+TGG - chr3_2:73057538-73057557 None:intergenic 25.0%
TTGTGTATTAAGAACAAGGA+TGG + chr3_2:73054124-73054143 Msa0423300:intron 30.0%
! ATTATTTTCCTGTTGCGTAT+AGG + chr3_2:73054174-73054193 Msa0423300:intron 30.0%
! CGTGTTTAAAATACGTGATT+AGG + chr3_2:73054233-73054252 Msa0423300:intron 30.0%
TTAGGACTTGATTAGGAATT+AGG + chr3_2:73054251-73054270 Msa0423300:intron 30.0%
! TTTGGAGTATTTATGAACAG+AGG + chr3_2:73054305-73054324 Msa0423300:intron 30.0%
! TTGGAGTATTTATGAACAGA+GGG + chr3_2:73054306-73054325 Msa0423300:intron 30.0%
AGGGAATAGATCTAAATTCA+AGG + chr3_2:73054325-73054344 Msa0423300:intron 30.0%
! GAGCTTTTATTAGCAATTGT+AGG + chr3_2:73054467-73054486 Msa0423300:intron 30.0%
TATTACCAACTTATCATGCA+AGG + chr3_2:73054634-73054653 Msa0423300:intron 30.0%
TTTGTAATCGTCTACATTTC+AGG + chr3_2:73054755-73054774 Msa0423300:intron 30.0%
TTGTAGTAGATTTCACATTG+TGG + chr3_2:73054867-73054886 Msa0423300:intron 30.0%
!!! TAGTCTATTTTAACTCCACA+GGG + chr3_2:73054930-73054949 Msa0423300:intron 30.0%
TTTGTTCTTAAGAGATTCTC+CGG + chr3_2:73055056-73055075 Msa0423300:intron 30.0%
CATAACTAAAAACCATATGC+TGG - chr3_2:73055276-73055295 None:intergenic 30.0%
!!! CAGCATATGGTTTTTAGTTA+TGG + chr3_2:73055274-73055293 Msa0423300:intron 30.0%
!!! TCTTTGTTTTTCTCTCAACT+TGG - chr3_2:73055304-73055323 None:intergenic 30.0%
! GAATTAACAAAGTTGACTCT+TGG - chr3_2:73055389-73055408 None:intergenic 30.0%
AGAGTCAACTTTGTTAATTC+TGG + chr3_2:73055389-73055408 Msa0423300:intron 30.0%
! ATTGTGTTCTTCTTTTTGTG+TGG + chr3_2:73055446-73055465 Msa0423300:intron 30.0%
! AAAGTAATGGAAGTTTGTGA+TGG + chr3_2:73055500-73055519 Msa0423300:intron 30.0%
!! TTAGCTTTAGAGGTGAAAAT+TGG + chr3_2:73055601-73055620 Msa0423300:intron 30.0%
!! ATATCGGTCATTTAGTTTGA+TGG - chr3_2:73055780-73055799 None:intergenic 30.0%
AAGTGGTAGTTTATATCACA+GGG - chr3_2:73055868-73055887 None:intergenic 30.0%
TAAGTGGTAGTTTATATCAC+AGG - chr3_2:73055869-73055888 None:intergenic 30.0%
AATCTTCGTTTAAGCTTAAG+TGG - chr3_2:73055885-73055904 None:intergenic 30.0%
AAGAATGTTACAACGATAAG+TGG + chr3_2:73056004-73056023 Msa0423300:intron 30.0%
TGGTTTACACAAGATAAGAT+AGG + chr3_2:73056024-73056043 Msa0423300:intron 30.0%
CTATACTCCATTTAGTCAAT+CGG - chr3_2:73056102-73056121 None:intergenic 30.0%
GGCGAGTTAAGAAAAATTAT+AGG + chr3_2:73056133-73056152 Msa0423300:intron 30.0%
CTCTAAATCTCTCATTAGTT+TGG - chr3_2:73056159-73056178 None:intergenic 30.0%
CACTGAATAATTGAAAACGT+CGG + chr3_2:73056403-73056422 Msa0423300:intron 30.0%
AATAGAAAACACACACAAAG+AGG - chr3_2:73056443-73056462 None:intergenic 30.0%
!! CAGCTTGCTAAGAAATTTAA+TGG - chr3_2:73056548-73056567 None:intergenic 30.0%
TAGTGTTAAGAAATGATTGC+CGG - chr3_2:73056583-73056602 None:intergenic 30.0%
CATTTCTTAACACTAACAAG+TGG + chr3_2:73056588-73056607 Msa0423300:CDS 30.0%
! CAGTGATGTTATTATCGTTT+TGG + chr3_2:73056637-73056656 Msa0423300:CDS 30.0%
!! GATGTTATTATCGTTTTGGT+TGG + chr3_2:73056641-73056660 Msa0423300:CDS 30.0%
TTATTGCCTAACAACTGAAT+TGG + chr3_2:73056938-73056957 Msa0423300:intron 30.0%
ACTTAACCAATTCAGTTGTT+AGG - chr3_2:73056947-73056966 None:intergenic 30.0%
! ACTTCAAAGTATGGCAATTT+TGG - chr3_2:73057051-73057070 None:intergenic 30.0%
ACATCATACATGAACATATG+GGG - chr3_2:73057536-73057555 None:intergenic 30.0%
TGTTCACTTGACACAATTAT+TGG + chr3_2:73057958-73057977 Msa0423300:three_prime_UTR 30.0%
TAAAATGGAAATATGCACCT+AGG + chr3_2:73057990-73058009 Msa0423300:three_prime_UTR 30.0%
! ATTGGTACAAAAGTACGTTT+GGG - chr3_2:73058021-73058040 None:intergenic 30.0%
TAAATTCGACACTACCTATC+AGG + chr3_2:73053859-73053878 Msa0423300:CDS 35.0%
GAAAAGTGGAATAACCTGAT+AGG - chr3_2:73053876-73053895 None:intergenic 35.0%
! GATATTTGTGTGATTTTGCG+AGG + chr3_2:73054026-73054045 Msa0423300:intron 35.0%
TACGTGATTAGGACTTGATT+AGG + chr3_2:73054244-73054263 Msa0423300:intron 35.0%
ATGCAGAATAAAATATCCGC+TGG - chr3_2:73054281-73054300 None:intergenic 35.0%
CACAAACAAGATACGAGTAT+TGG + chr3_2:73054544-73054563 Msa0423300:intron 35.0%
! GATAAGTATCGTAACTGATG+CGG + chr3_2:73054566-73054585 Msa0423300:intron 35.0%
AGTAACCTTGCATGATAAGT+TGG - chr3_2:73054642-73054661 None:intergenic 35.0%
! TCTCCTTTGTCATGTTGTTT+CGG + chr3_2:73054677-73054696 Msa0423300:intron 35.0%
AAACCGAAACAACATGACAA+AGG - chr3_2:73054683-73054702 None:intergenic 35.0%
AAGATCCTAACAGTAGGAAA+GGG - chr3_2:73054728-73054747 None:intergenic 35.0%
TTTGTCAAGATCCTAACAGT+AGG - chr3_2:73054734-73054753 None:intergenic 35.0%
!!! GTAGTCTATTTTAACTCCAC+AGG + chr3_2:73054929-73054948 Msa0423300:intron 35.0%
TATAAGAGATTCTTCAGTCG+CGG + chr3_2:73054996-73055015 Msa0423300:CDS 35.0%
TCATATACAATAACCGCAAC+CGG - chr3_2:73055078-73055097 None:intergenic 35.0%
ACTAAAAACCATATGCTGGA+TGG - chr3_2:73055272-73055291 None:intergenic 35.0%
AACACAATGACATTGTCTCT+TGG - chr3_2:73055434-73055453 None:intergenic 35.0%
GTGGATAATATAGTCCTAGT+TGG + chr3_2:73055465-73055484 Msa0423300:intron 35.0%
TGAAGTACAAGTTTAGCAAG+AGG + chr3_2:73055571-73055590 Msa0423300:intron 35.0%
ATAGAGAATAGAGAGAGACA+TGG - chr3_2:73055747-73055766 None:intergenic 35.0%
!! ATTTAGTTTGATGGAGCTGT+CGG - chr3_2:73055771-73055790 None:intergenic 35.0%
AGTTGGCATCTCCTATATAT+CGG - chr3_2:73055796-73055815 None:intergenic 35.0%
ATATAGGAGATGCCAACTAT+AGG + chr3_2:73055798-73055817 Msa0423300:intron 35.0%
AGCCAAAAAGAAAGATGTCT+CGG + chr3_2:73055969-73055988 Msa0423300:intron 35.0%
!! AAAAGCACCGATTGACTAAA+TGG + chr3_2:73056092-73056111 Msa0423300:intron 35.0%
ATGGAGTATAGTCCAACATA+AGG + chr3_2:73056111-73056130 Msa0423300:intron 35.0%
TGGAGTATAGTCCAACATAA+GGG + chr3_2:73056112-73056131 Msa0423300:intron 35.0%
CATGGATCAAGTGTTAAGAT+AGG - chr3_2:73056191-73056210 None:intergenic 35.0%
AACAGAAATAACAGCAAAGC+AGG - chr3_2:73056263-73056282 None:intergenic 35.0%
TTGTTGAAAAAAGGTGAGCA+GGG + chr3_2:73056678-73056697 Msa0423300:intron 35.0%
!! CAGTTGATAGGTAACTATCA+AGG - chr3_2:73056733-73056752 None:intergenic 35.0%
! CTTGATAGTTACCTATCAAC+TGG + chr3_2:73056731-73056750 Msa0423300:intron 35.0%
TAGGTCCATGATGATATTGT+TGG + chr3_2:73057105-73057124 Msa0423300:intron 35.0%
TAATAGGCAAGTCTCTATAG+AGG + chr3_2:73057409-73057428 Msa0423300:intron 35.0%
!! ATTAAATCCTGCTTTTGCAC+TGG - chr3_2:73057480-73057499 None:intergenic 35.0%
AGACAGACTTCAGAATTCAA+AGG - chr3_2:73057572-73057591 None:intergenic 35.0%
! ATCTAAAAGTATGCCATCCA+TGG + chr3_2:73057649-73057668 Msa0423300:intron 35.0%
! ACAAGTTTGAAATCCATGGA+TGG - chr3_2:73057665-73057684 None:intergenic 35.0%
! GACAACAAGTTTGAAATCCA+TGG - chr3_2:73057669-73057688 None:intergenic 35.0%
!! CTGCAACATTTTAATCACTC+GGG - chr3_2:73057768-73057787 None:intergenic 35.0%
!! GCTGCAACATTTTAATCACT+CGG - chr3_2:73057769-73057788 None:intergenic 35.0%
GAAGCAATCTTGCGAAATAA+AGG - chr3_2:73057791-73057810 None:intergenic 35.0%
TTCAACAAAGCAAGAAGACA+TGG + chr3_2:73057835-73057854 Msa0423300:CDS 35.0%
! AAACAGTGCTATTCACAACA+AGG + chr3_2:73057927-73057946 Msa0423300:exon 35.0%
! ACTTGACACAATTATTGGCT+TGG + chr3_2:73057963-73057982 Msa0423300:three_prime_UTR 35.0%
!! TATTGGCTTGGCAACTAAAA+TGG + chr3_2:73057975-73057994 Msa0423300:three_prime_UTR 35.0%
! TACAAAAGTACGTTTGGGTT+TGG - chr3_2:73058016-73058035 None:intergenic 35.0%
! CATTGGTACAAAAGTACGTT+TGG - chr3_2:73058022-73058041 None:intergenic 35.0%
! CAAACTAAACTGAAGAGCAT+TGG - chr3_2:73058039-73058058 None:intergenic 35.0%
CAATGCTCTTCAGTTTAGTT+TGG + chr3_2:73058037-73058056 Msa0423300:three_prime_UTR 35.0%
AACGTGAATAACGCGAATCT+GGG - chr3_2:73053708-73053727 None:intergenic 40.0%
ACGAGTTTGTATTTGGCTAG+AGG - chr3_2:73053782-73053801 None:intergenic 40.0%
TTCTTAGGTGATCAATCCGT+TGG + chr3_2:73053803-73053822 Msa0423300:CDS 40.0%
!!! GATGATGCTGGTTTTACCAA+CGG - chr3_2:73053822-73053841 None:intergenic 40.0%
! TTGTGTGATTTTGCGAGGAT+CGG + chr3_2:73054031-73054050 Msa0423300:intron 40.0%
GGACTTGATTAGGAATTAGG+AGG + chr3_2:73054254-73054273 Msa0423300:intron 40.0%
AATGCACGAGTAAAGCAAGA+AGG - chr3_2:73054400-73054419 None:intergenic 40.0%
!! CGTTAAAGAAGTGCTAGAGT+AGG + chr3_2:73054439-73054458 Msa0423300:intron 40.0%
AGATCCTAACAGTAGGAAAG+GGG - chr3_2:73054727-73054746 None:intergenic 40.0%
CAAGATCCTAACAGTAGGAA+AGG - chr3_2:73054729-73054748 None:intergenic 40.0%
TCTACATTTCAGGCGACAAT+TGG + chr3_2:73054765-73054784 Msa0423300:intron 40.0%
AACGAACTGTTCGATCTTCA+AGG - chr3_2:73054818-73054837 None:intergenic 40.0%
AACTCTCTTCTGATAGGACA+TGG - chr3_2:73055246-73055265 None:intergenic 40.0%
TGGTCAAACTCTCTTCTGAT+AGG - chr3_2:73055252-73055271 None:intergenic 40.0%
ATATAGTCCTAGTTGGAGAC+TGG + chr3_2:73055472-73055491 Msa0423300:intron 40.0%
GAGACTGGAGAAGAAAGTAA+TGG + chr3_2:73055487-73055506 Msa0423300:intron 40.0%
! GTTTGTGATGGAGTCAGTAT+TGG + chr3_2:73055512-73055531 Msa0423300:intron 40.0%
GAATTGAGAGAGATCTGATG+AGG + chr3_2:73055718-73055737 Msa0423300:intron 40.0%
CCAACTATAGGATCCAAGTA+GGG + chr3_2:73055810-73055829 Msa0423300:intron 40.0%
! TCTATTTTGTCCACCCTACT+TGG - chr3_2:73055826-73055845 None:intergenic 40.0%
AAGTAGGGTGGACAAAATAG+AGG + chr3_2:73055825-73055844 Msa0423300:intron 40.0%
! CACCGAGACATCTTTCTTTT+TGG - chr3_2:73055974-73055993 None:intergenic 40.0%
TTCTTAACTCGCCCTTATGT+TGG - chr3_2:73056126-73056145 None:intergenic 40.0%
ACACAAAGAGGTAGAGAACA+GGG - chr3_2:73056431-73056450 None:intergenic 40.0%
!! TAACACTAACAAGTGGGTTG+AGG + chr3_2:73056595-73056614 Msa0423300:CDS 40.0%
CTTGTTGAAAAAAGGTGAGC+AGG + chr3_2:73056677-73056696 Msa0423300:intron 40.0%
AAAATGCTCAATGTCCTGAC+TGG - chr3_2:73056710-73056729 None:intergenic 40.0%
AAGTCAGCAAACCAGTTGAT+AGG - chr3_2:73056745-73056764 None:intergenic 40.0%
ACATCACATGCAAAGCTAAC+CGG - chr3_2:73057142-73057161 None:intergenic 40.0%
GTATGGATATGTGCCATATG+AGG - chr3_2:73057327-73057346 None:intergenic 40.0%
ATAGAAACCAGTGCAAAAGC+AGG + chr3_2:73057470-73057489 Msa0423300:CDS 40.0%
ATGCAGCTGAGAATCATTCA+AGG - chr3_2:73057620-73057639 None:intergenic 40.0%
TGTTGAAAGACTTTCCATCC+CGG - chr3_2:73057822-73057841 None:intergenic 40.0%
AATCTACCGTGAATTCATCC+AGG + chr3_2:73057873-73057892 Msa0423300:CDS 40.0%
!! GCTCTTCAGTTTAGTTTGGT+AGG + chr3_2:73058041-73058060 Msa0423300:three_prime_UTR 40.0%
GAACGTGAATAACGCGAATC+TGG - chr3_2:73053709-73053728 None:intergenic 45.0%
GCTTGTTTCCTATACGCAAC+AGG - chr3_2:73054185-73054204 None:intergenic 45.0%
TTAGGAATTAGGAGGACCAG+CGG + chr3_2:73054262-73054281 Msa0423300:intron 45.0%
TTTCCCCCTTTCCTACTGTT+AGG + chr3_2:73054720-73054739 Msa0423300:intron 45.0%
GATCCTAACAGTAGGAAAGG+GGG - chr3_2:73054726-73054745 None:intergenic 45.0%
CTTAAGAGATTCTCCGGTTG+CGG + chr3_2:73055062-73055081 Msa0423300:intron 45.0%
GAGTTTGACCATCCAGCATA+TGG + chr3_2:73055261-73055280 Msa0423300:intron 45.0%
TTCTTCTCCAGTCTCCAACT+AGG - chr3_2:73055482-73055501 None:intergenic 45.0%
!! AGGCACGAGTTTAGCTTTAG+AGG + chr3_2:73055591-73055610 Msa0423300:intron 45.0%
GCCAACTATAGGATCCAAGT+AGG + chr3_2:73055809-73055828 Msa0423300:intron 45.0%
CCCTACTTGGATCCTATAGT+TGG - chr3_2:73055813-73055832 None:intergenic 45.0%
ACTATAGGATCCAAGTAGGG+TGG + chr3_2:73055813-73055832 Msa0423300:intron 45.0%
TTCTTGGAAGATAGCACGTG+TGG + chr3_2:73056060-73056079 Msa0423300:intron 45.0%
AAAGCAGGCTTCCAATAGCT+GGG - chr3_2:73056248-73056267 None:intergenic 45.0%
CACACAAAGAGGTAGAGAAC+AGG - chr3_2:73056432-73056451 None:intergenic 45.0%
AGGCAAGTCTCTATAGAGGA+AGG + chr3_2:73057413-73057432 Msa0423300:intron 45.0%
CATGAACATATGGGGTGACT+CGG - chr3_2:73057528-73057547 None:intergenic 45.0%
GTTGAAAGACTTTCCATCCC+GGG - chr3_2:73057821-73057840 None:intergenic 45.0%
CTCTGTCCTGGATGAATTCA+CGG - chr3_2:73057882-73057901 None:intergenic 45.0%
! CATGAAGCGAGTGATGATGC+TGG - chr3_2:73053834-73053853 None:intergenic 50.0%
ACTGATGCGGCGATACGTTA+AGG + chr3_2:73054579-73054598 Msa0423300:intron 50.0%
GAACAGTTCGTTTGCAGCTC+TGG + chr3_2:73054826-73054845 Msa0423300:CDS 50.0%
TAACTCCACAGGGATACTGC+AGG + chr3_2:73054940-73054959 Msa0423300:intron 50.0%
AACTCCACAGGGATACTGCA+GGG + chr3_2:73054941-73054960 Msa0423300:intron 50.0%
TCTAGGTACTCCCCAGCTAT+TGG + chr3_2:73056234-73056253 Msa0423300:intron 50.0%
AAGCAGGCTTCCAATAGCTG+GGG - chr3_2:73056247-73056266 None:intergenic 50.0%
CAAAGCAGGCTTCCAATAGC+TGG - chr3_2:73056249-73056268 None:intergenic 50.0%
CAAGCTGTTCTGATGCAGAC+CGG + chr3_2:73056561-73056580 Msa0423300:intron 50.0%
!! CACTAACAAGTGGGTTGAGG+AGG + chr3_2:73056598-73056617 Msa0423300:CDS 50.0%
AAGATTGCTTCAGCCTTGCC+CGG + chr3_2:73057800-73057819 Msa0423300:CDS 50.0%
! AGTACGTTTGGGTTTGGCCT+AGG - chr3_2:73058010-73058029 None:intergenic 50.0%
AGATTGCTTCAGCCTTGCCC+GGG + chr3_2:73057801-73057820 Msa0423300:CDS 55.0%
AAGACTTTCCATCCCGGGCA+AGG - chr3_2:73057816-73057835 None:intergenic 55.0%
TCATCCAGGACAGAGCAGCA+AGG + chr3_2:73057887-73057906 Msa0423300:CDS 55.0%
CTTGCCCTGCAGTATCCCTG+TGG - chr3_2:73054948-73054967 None:intergenic 60.0%
! GAGGAGGTACGTGCAGAACG+AGG + chr3_2:73056614-73056633 Msa0423300:CDS 60.0%
GAGCAGGGAGTCAACCAGTC+AGG + chr3_2:73056693-73056712 Msa0423300:intron 60.0%
GATGCCAAGTCGCGTGAGAG+CGG + chr3_2:73057437-73057456 Msa0423300:CDS 60.0%
ATGCCAAGTCGCGTGAGAGC+GGG + chr3_2:73057438-73057457 Msa0423300:CDS 60.0%
TCCAGGACAGAGCAGCAAGG+TGG + chr3_2:73057890-73057909 Msa0423300:CDS 60.0%
TCCACCTTGCTGCTCTGTCC+TGG - chr3_2:73057894-73057913 None:intergenic 60.0%
AGGACAGAGCAGCAAGGTGG+AGG + chr3_2:73057893-73057912 Msa0423300:CDS 60.0%
GATCCCGCTCTCACGCGACT+TGG - chr3_2:73057444-73057463 None:intergenic 65.0%
TGCTTCAGCCTTGCCCGGGA+TGG + chr3_2:73057805-73057824 Msa0423300:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 73053653 73058122 73053653 ID=Msa0423300;Name=Msa0423300
chr3_2 mRNA 73053653 73058122 73053653 ID=Msa0423300-mRNA-1;Parent=Msa0423300;Name=Msa0423300-mRNA-1;_AED=0.08;_eAED=0.08;_QI=111|0.8|0.66|1|1|1|6|194|206
chr3_2 exon 73053653 73053880 73053653 ID=Msa0423300-mRNA-1:exon:18132;Parent=Msa0423300-mRNA-1
chr3_2 exon 73054777 73054847 73054777 ID=Msa0423300-mRNA-1:exon:18133;Parent=Msa0423300-mRNA-1
chr3_2 exon 73054951 73055039 73054951 ID=Msa0423300-mRNA-1:exon:18134;Parent=Msa0423300-mRNA-1
chr3_2 exon 73056579 73056690 73056579 ID=Msa0423300-mRNA-1:exon:18135;Parent=Msa0423300-mRNA-1
chr3_2 exon 73057415 73057505 73057415 ID=Msa0423300-mRNA-1:exon:18136;Parent=Msa0423300-mRNA-1
chr3_2 exon 73057788 73058122 73057788 ID=Msa0423300-mRNA-1:exon:18137;Parent=Msa0423300-mRNA-1
chr3_2 five_prime_UTR 73053653 73053763 73053653 ID=Msa0423300-mRNA-1:five_prime_utr;Parent=Msa0423300-mRNA-1
chr3_2 CDS 73053764 73053880 73053764 ID=Msa0423300-mRNA-1:cds;Parent=Msa0423300-mRNA-1
chr3_2 CDS 73054777 73054847 73054777 ID=Msa0423300-mRNA-1:cds;Parent=Msa0423300-mRNA-1
chr3_2 CDS 73054951 73055039 73054951 ID=Msa0423300-mRNA-1:cds;Parent=Msa0423300-mRNA-1
chr3_2 CDS 73056579 73056690 73056579 ID=Msa0423300-mRNA-1:cds;Parent=Msa0423300-mRNA-1
chr3_2 CDS 73057415 73057505 73057415 ID=Msa0423300-mRNA-1:cds;Parent=Msa0423300-mRNA-1
chr3_2 CDS 73057788 73057928 73057788 ID=Msa0423300-mRNA-1:cds;Parent=Msa0423300-mRNA-1
chr3_2 three_prime_UTR 73057929 73058122 73057929 ID=Msa0423300-mRNA-1:three_prime_utr;Parent=Msa0423300-mRNA-1
Gene Sequence

>Msa0423300

ATGGTGACTGTATCACCTCTAGCCAAATACAAACTCGTTTTCTTAGGTGATCAATCCGTTGGTAAAACCAGCATCATCACTCGCTTCATGTATGATAAATTCGACACTACCTATCAGGCGACAATTGGTATTGACTTTTTGTCGAAAACAATGTACCTTGAAGATCGAACAGTTCGTTTGCAGCTCTGGGATACTGCAGGGCAAGAAAGATTTAGAAGTCTCATTCCAAGTTATATAAGAGATTCTTCAGTCGCGGTTATTGTGTATGATGTTGCCAACCGGCAATCATTTCTTAACACTAACAAGTGGGTTGAGGAGGTACGTGCAGAACGAGGCAGTGATGTTATTATCGTTTTGGTTGGAAACAAAACTGATCTTGTTGAAAAAAGGCAAGTCTCTATAGAGGAAGGAGATGCCAAGTCGCGTGAGAGCGGGATCATGTTTATAGAAACCAGTGCAAAAGCAGGATTTAATATCAAGCCTTTATTTCGCAAGATTGCTTCAGCCTTGCCCGGGATGGAAAGTCTTTCAACAAAGCAAGAAGACATGGTTGATGTAAATTTAAAATCTACCGTGAATTCATCCAGGACAGAGCAGCAAGGTGGAGGTTGTTCGTGCTAA
Protein sequence

>Msa0423300

MVTVSPLAKYKLVFLGDQSVGKTSIITRFMYDKFDTTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSSVAVIVYDVANRQSFLNTNKWVEEVRAERGSDVIIVLVGNKTDLVEKRQVSIEEGDAKSRESGIMFIETSAKAGFNIKPLFRKIASALPGMESLSTKQEDMVDVNLKSTVNSSRTEQQGGGCSC*