AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0515070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515070 MtrunA17_Chr3g0123971 98.544 206 3 0 1 206 1 206 2.03e-150 415
Msa0515070 MtrunA17_Chr8g0382221 94.686 207 10 1 1 206 1 207 5.16e-144 399
Msa0515070 MtrunA17_Chr5g0400131 84.541 207 31 1 1 206 1 207 1.14e-129 363
Msa0515070 MtrunA17_Chr4g0055081 85.507 207 28 2 1 206 1 206 3.18e-129 362
Msa0515070 MtrunA17_Chr1g0199331 47.561 164 85 1 9 171 31 194 3.79e-51 164
Msa0515070 MtrunA17_Chr5g0433561 44.099 161 90 0 11 171 12 172 1.54e-49 159
Msa0515070 MtrunA17_Chr3g0109721 43.478 161 91 0 11 171 12 172 2.92e-48 156
Msa0515070 MtrunA17_Chr8g0390561 44.375 160 89 0 11 170 11 170 5.44e-47 153
Msa0515070 MtrunA17_Chr8g0349711 40.341 176 103 1 10 185 7 180 5.23e-46 151
Msa0515070 MtrunA17_Chr4g0032201 41.401 157 92 0 10 166 13 169 2.33e-45 149
Msa0515070 MtrunA17_Chr8g0349681 40.909 176 102 1 10 185 176 349 5.11e-44 154
Msa0515070 MtrunA17_Chr8g0349681 42.197 173 94 1 10 182 385 551 7.19e-41 145
Msa0515070 MtrunA17_Chr8g0349681 40.123 162 97 0 10 171 14 175 2.98e-40 144
Msa0515070 MtrunA17_Chr6g0458081 35.533 197 119 2 10 203 7 198 6.72e-44 145
Msa0515070 MtrunA17_Chr8g0351861 37.888 161 100 0 10 170 17 177 8.97e-44 146
Msa0515070 MtrunA17_Chr2g0276931 43.478 161 91 0 10 170 10 170 1.12e-43 145
Msa0515070 MtrunA17_Chr2g0276941 37.158 183 112 1 10 189 16 198 1.93e-43 145
Msa0515070 MtrunA17_Chr8g0346421 42.038 157 91 0 10 166 27 183 4.33e-43 144
Msa0515070 MtrunA17_Chr3g0123821 34.518 197 114 1 10 206 17 198 6.84e-43 143
Msa0515070 MtrunA17_Chr7g0234201 41.558 154 90 0 10 163 14 167 1.10e-42 142
Msa0515070 MtrunA17_Chr2g0290891 37.267 161 101 0 10 170 7 167 1.39e-42 142
Msa0515070 MtrunA17_Chr3g0112681 37.640 178 102 1 10 178 13 190 3.41e-42 141
Msa0515070 MtrunA17_Chr7g0231131 34.896 192 112 1 10 201 16 194 3.42e-42 141
Msa0515070 MtrunA17_Chr8g0381841 34.010 197 115 1 10 206 18 199 8.54e-42 140
Msa0515070 MtrunA17_Chr7g0250531 42.236 161 93 0 10 170 9 169 1.19e-41 139
Msa0515070 MtrunA17_Chr7g0214241 35.961 203 124 2 10 206 16 218 1.53e-41 140
Msa0515070 MtrunA17_Chr4g0055651 41.615 161 94 0 10 170 9 169 1.66e-41 139
Msa0515070 MtrunA17_Chr6g0482721 37.356 174 109 0 10 183 13 186 2.14e-41 139
Msa0515070 MtrunA17_Chr1g0174281 35.266 207 122 3 10 204 16 222 2.51e-41 139
Msa0515070 MtrunA17_Chr7g0275991 38.182 165 98 1 6 166 13 177 4.20e-41 139
Msa0515070 MtrunA17_Chr8g0335471 40.994 161 95 0 10 170 9 169 1.84e-40 136
Msa0515070 MtrunA17_Chr1g0189361 38.217 157 97 0 10 166 15 171 2.60e-40 137
Msa0515070 MtrunA17_Chr4g0030911 36.548 197 122 2 11 204 17 213 4.84e-40 136
Msa0515070 MtrunA17_Chr4g0046261 36.306 157 100 0 10 166 14 170 8.54e-40 135
Msa0515070 MtrunA17_Chr2g0315551 35.669 157 101 0 10 166 14 170 1.18e-39 135
Msa0515070 MtrunA17_Chr1g0212521 36.364 198 122 3 11 204 17 214 1.37e-39 135
Msa0515070 MtrunA17_Chr1g0182321 42.405 158 90 1 11 167 17 174 3.00e-39 134
Msa0515070 MtrunA17_Chr2g0327911 37.056 197 120 3 11 204 17 212 3.25e-39 134
Msa0515070 MtrunA17_Chr1g0182301 42.405 158 90 1 11 167 17 174 4.82e-39 133
Msa0515070 MtrunA17_Chr4g0040781 36.306 157 100 0 10 166 14 170 4.85e-39 133
Msa0515070 MtrunA17_Chr7g0263071 42.405 158 90 1 11 167 17 174 5.09e-39 133
Msa0515070 MtrunA17_Chr2g0283131 36.306 157 100 0 10 166 14 170 8.49e-39 132
Msa0515070 MtrunA17_Chr2g0319951 34.783 161 105 0 10 170 14 174 1.88e-38 132
Msa0515070 MtrunA17_Chr2g0325091 33.663 202 128 2 10 205 12 213 2.44e-38 131
Msa0515070 MtrunA17_Chr4g0004661 39.216 204 110 5 11 206 66 263 2.82e-38 132
Msa0515070 MtrunA17_Chr4g0034871 38.235 204 111 4 11 206 10 206 4.48e-38 130
Msa0515070 MtrunA17_Chr2g0331691 36.946 203 116 3 11 206 12 209 1.79e-37 129
Msa0515070 MtrunA17_Chr8g0383261 33.498 203 121 4 11 206 14 209 5.45e-36 125
Msa0515070 MtrunA17_Chr1g0197331 37.186 199 120 4 10 205 16 212 1.64e-35 124
Msa0515070 MtrunA17_Chr8g0384311 41.509 159 90 3 10 166 14 171 2.02e-35 124
Msa0515070 MtrunA17_Chr1g0201121 34.975 203 120 4 11 206 10 207 2.31e-35 124
Msa0515070 MtrunA17_Chr6g0466301 35.294 204 117 5 11 206 10 206 2.61e-35 123
Msa0515070 MtrunA17_Chr6g0465491 35.294 204 117 5 11 206 10 206 2.61e-35 123
Msa0515070 MtrunA17_Chr1g0180291 36.041 197 123 3 10 204 15 210 2.89e-35 123
Msa0515070 MtrunA17_Chr8g0392931 37.267 161 101 0 10 170 15 175 3.58e-35 123
Msa0515070 MtrunA17_Chr7g0265001 33.333 198 130 2 10 205 17 214 7.68e-34 120
Msa0515070 MtrunA17_Chr3g0130271 39.412 170 99 4 10 176 13 181 1.65e-32 116
Msa0515070 MtrunA17_Chr1g0159481 37.278 169 103 3 10 176 18 185 5.22e-32 115
Msa0515070 MtrunA17_Chr3g0138121 32.143 168 111 3 10 177 14 178 1.02e-24 96.7
Msa0515070 MtrunA17_Chr3g0138111 32.143 168 111 3 10 177 14 178 1.04e-24 96.7
Msa0515070 MtrunA17_Chr3g0138101 32.143 168 111 3 10 177 14 178 1.05e-24 96.7
Msa0515070 MtrunA17_Chr3g0117931 35.758 165 92 5 11 163 10 172 6.69e-22 89.0
Msa0515070 MtrunA17_Chr2g0325101 35.152 165 93 5 11 163 8 170 1.35e-21 87.8
Msa0515070 MtrunA17_Chr6g0486041 34.545 165 94 5 11 163 8 170 3.62e-20 84.3
Msa0515070 MtrunA17_Chr6g0466161 28.191 188 121 5 26 206 7 187 9.60e-20 83.2
Msa0515070 MtrunA17_Chr4g0046211 36.154 130 80 3 11 139 8 135 2.45e-19 80.5
Msa0515070 MtrunA17_Chr8g0371501 30.909 165 100 4 11 163 8 170 4.51e-19 81.3
Msa0515070 MtrunA17_Chr6g0465471 27.660 188 122 5 26 206 7 187 4.76e-19 81.3
Msa0515070 MtrunA17_Chr5g0405791 30.909 165 100 4 11 163 8 170 5.34e-19 80.9
Msa0515070 MtrunA17_Chr4g0037021 31.515 165 99 5 11 163 8 170 1.09e-18 80.1
Msa0515070 MtrunA17_Chr6g0466431 38.542 96 50 1 11 106 10 96 4.95e-18 75.9
Msa0515070 MtrunA17_Chr6g0465611 38.542 96 50 1 11 106 10 96 4.95e-18 75.9
Msa0515070 MtrunA17_Chr5g0443631 25.146 171 122 3 5 170 100 269 1.30e-13 67.8
Msa0515070 MtrunA17_Chr1g0178291 32.051 156 97 4 12 166 431 578 4.42e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515070 AT5G10260.1 88.406 207 23 1 1 206 1 207 1.09e-136 381
Msa0515070 AT2G22290.1 88.889 207 22 1 1 206 1 207 1.89e-135 378
Msa0515070 AT2G44610.1 86.058 208 27 2 1 206 1 208 4.13e-131 367
Msa0515070 AT4G39890.1 78.972 214 37 3 1 206 1 214 2.58e-117 332
Msa0515070 AT5G64990.1 78.392 199 41 2 10 206 8 206 3.23e-108 309
Msa0515070 AT5G64990.2 75.728 206 41 3 10 206 8 213 2.37e-105 302
Msa0515070 AT4G19640.1 44.720 161 89 0 11 171 12 172 1.12e-50 163
Msa0515070 AT3G54840.1 47.561 164 85 1 9 171 33 196 1.43e-50 162
Msa0515070 AT5G45130.1 44.720 161 89 0 11 171 12 172 1.45e-50 162
Msa0515070 AT3G54840.3 47.826 161 83 1 12 171 2 162 5.73e-50 160
Msa0515070 AT5G45130.2 44.025 159 89 0 13 171 22 180 1.18e-48 158
Msa0515070 AT3G54840.2 48.077 156 80 1 9 163 33 188 1.90e-48 157
Msa0515070 AT1G09630.1 40.252 159 95 0 10 168 13 171 9.24e-44 145
Msa0515070 AT3G11730.1 38.889 198 114 2 10 204 9 202 1.23e-43 145
Msa0515070 AT5G65270.1 38.217 157 97 0 10 166 18 174 1.41e-43 145
Msa0515070 AT1G07410.1 39.752 161 97 0 10 170 13 173 1.61e-43 145
Msa0515070 AT4G17160.1 38.272 162 100 0 9 170 6 167 2.54e-43 144
Msa0515070 AT1G05810.1 37.255 204 119 3 10 206 13 214 2.54e-43 145
Msa0515070 AT4G35860.1 34.615 208 118 3 10 204 7 209 3.40e-43 144
Msa0515070 AT1G05810.2 37.255 204 119 3 10 206 59 260 6.75e-43 145
Msa0515070 AT4G39990.1 36.646 161 102 0 10 170 18 178 1.28e-42 143
Msa0515070 AT4G17170.1 37.267 161 101 0 10 170 7 167 1.65e-42 142
Msa0515070 AT3G12160.1 36.946 203 120 3 10 204 16 218 1.85e-42 142
Msa0515070 AT5G59150.1 39.490 157 95 0 10 166 13 169 2.04e-42 142
Msa0515070 AT2G31680.1 35.122 205 123 3 10 206 13 215 4.25e-42 142
Msa0515070 AT3G46830.1 38.854 157 96 0 10 166 13 169 4.78e-42 141
Msa0515070 AT3G46830.2 38.854 157 96 0 10 166 13 169 4.78e-42 141
Msa0515070 AT3G07410.1 42.208 154 89 0 10 163 13 166 8.04e-42 141
Msa0515070 AT5G47960.1 34.804 204 124 2 10 204 16 219 1.45e-41 140
Msa0515070 AT1G18200.1 40.909 154 91 0 10 163 14 167 2.09e-41 140
Msa0515070 AT1G02130.1 40.994 161 95 0 10 170 9 169 3.56e-41 139
Msa0515070 AT5G47520.1 38.217 157 97 0 10 166 15 171 8.39e-41 138
Msa0515070 AT2G43130.1 38.217 157 97 0 10 166 13 169 1.05e-40 138
Msa0515070 AT3G15060.1 36.646 161 102 0 10 170 14 174 2.24e-40 137
Msa0515070 AT4G17530.1 40.373 161 96 0 10 170 9 169 4.03e-40 136
Msa0515070 AT5G47200.1 40.373 161 96 0 10 170 9 169 4.49e-40 136
Msa0515070 AT4G18430.1 33.010 206 125 3 10 205 14 216 1.10e-39 135
Msa0515070 AT5G60860.1 36.025 161 103 0 10 170 14 174 1.13e-39 135
Msa0515070 AT3G18820.1 38.725 204 110 4 11 206 10 206 1.17e-39 135
Msa0515070 AT1G73640.1 38.961 154 94 0 10 163 14 167 1.74e-39 135
Msa0515070 AT4G19640.2 45.238 126 69 0 11 136 12 137 3.56e-39 132
Msa0515070 AT1G28550.1 37.580 157 98 0 10 166 14 170 5.91e-39 134
Msa0515070 AT5G59840.1 37.056 197 111 3 11 203 17 204 7.74e-39 133
Msa0515070 AT1G16920.1 35.032 157 102 0 10 166 14 170 8.71e-39 133
Msa0515070 AT3G46060.2 41.772 158 91 1 11 167 17 174 2.38e-38 132
Msa0515070 AT3G46060.3 41.772 158 91 1 11 167 17 174 2.38e-38 132
Msa0515070 AT3G46060.1 41.772 158 91 1 11 167 17 174 2.38e-38 132
Msa0515070 AT1G06400.1 36.306 157 100 0 10 166 14 170 2.85e-38 132
Msa0515070 AT1G01200.1 36.190 210 120 5 10 206 29 237 2.87e-38 132
Msa0515070 AT4G19640.3 45.600 125 68 0 11 135 12 136 4.86e-38 130
Msa0515070 AT1G52280.1 38.235 204 111 4 11 206 10 206 6.19e-38 130
Msa0515070 AT3G53610.2 41.772 158 91 1 11 167 17 174 7.05e-38 130
Msa0515070 AT3G53610.3 41.772 158 91 1 11 167 17 174 7.05e-38 130
Msa0515070 AT3G53610.1 41.772 158 91 1 11 167 17 174 7.05e-38 130
Msa0515070 AT5G03520.1 38.889 198 118 3 11 205 17 214 1.03e-37 130
Msa0515070 AT3G16100.1 38.725 204 110 4 11 206 10 206 3.75e-37 129
Msa0515070 AT5G45750.1 35.220 159 99 2 10 166 14 170 3.75e-37 129
Msa0515070 AT2G33870.1 36.306 157 100 0 10 166 14 170 7.30e-37 128
Msa0515070 AT2G21880.1 35.714 210 113 5 11 206 11 212 2.34e-36 127
Msa0515070 AT4G18800.1 34.591 159 100 2 10 166 14 170 5.96e-36 125
Msa0515070 AT1G49300.2 36.275 204 115 4 11 206 10 206 2.27e-35 124
Msa0515070 AT1G49300.1 36.275 204 115 4 11 206 10 206 2.27e-35 124
Msa0515070 AT3G09900.1 43.038 158 89 1 11 167 17 174 4.74e-35 123
Msa0515070 AT4G09720.1 36.275 204 115 7 11 206 10 206 1.15e-34 122
Msa0515070 AT1G22740.1 35.468 203 115 5 11 206 10 203 2.92e-34 121
Msa0515070 AT5G03530.2 34.848 198 126 3 10 205 14 210 8.77e-34 120
Msa0515070 AT5G03530.1 34.848 198 126 3 10 205 14 210 8.77e-34 120
Msa0515070 AT4G09720.4 35.407 209 115 7 11 206 10 211 2.72e-33 119
Msa0515070 AT1G43890.3 37.791 172 104 3 10 179 14 184 3.04e-33 119
Msa0515070 AT1G43890.1 37.791 172 104 3 10 179 14 184 3.04e-33 119
Msa0515070 AT1G43890.2 37.791 172 104 3 10 179 14 184 3.04e-33 119
Msa0515070 AT4G09720.3 34.419 215 115 7 11 206 10 217 1.20e-32 117
Msa0515070 AT4G35860.2 36.364 165 87 3 53 204 4 163 1.61e-32 115
Msa0515070 AT5G39620.1 35.922 206 118 6 9 206 5 204 4.12e-32 115
Msa0515070 AT5G03520.2 37.576 165 100 3 44 205 40 204 4.63e-30 110
Msa0515070 AT3G09910.3 36.869 198 116 5 10 205 14 204 6.64e-30 110
Msa0515070 AT3G09910.1 36.869 198 116 5 10 205 14 204 6.64e-30 110
Msa0515070 AT3G09910.2 36.869 198 116 5 10 205 14 204 6.64e-30 110
Msa0515070 AT2G21880.4 33.846 195 107 5 26 206 1 187 2.07e-29 108
Msa0515070 AT2G21880.3 33.846 195 107 5 26 206 1 187 2.07e-29 108
Msa0515070 AT3G09910.5 38.750 160 95 3 10 167 14 172 3.54e-29 107
Msa0515070 AT3G09910.4 38.750 160 95 3 10 167 14 172 3.54e-29 107
Msa0515070 AT5G59150.2 39.474 114 69 0 53 166 5 118 6.81e-28 103
Msa0515070 AT2G21880.2 32.381 210 112 6 11 206 11 204 6.22e-27 102
Msa0515070 AT4G09720.6 35.593 177 99 7 38 206 3 172 1.85e-25 97.8
Msa0515070 AT4G09720.5 35.593 177 99 7 38 206 3 172 1.85e-25 97.8
Msa0515070 AT4G09720.2 35.593 177 99 7 38 206 3 172 1.85e-25 97.8
Msa0515070 AT5G55080.1 30.409 171 116 3 7 177 11 178 2.09e-25 99.0
Msa0515070 AT5G20020.1 32.143 168 111 3 10 177 14 178 5.72e-25 97.8
Msa0515070 AT5G20010.1 32.143 168 111 3 10 177 14 178 7.86e-25 97.4
Msa0515070 AT5G55190.1 32.143 168 111 3 10 177 14 178 1.72e-24 96.3
Msa0515070 AT2G44690.1 35.152 165 93 5 11 163 20 182 9.66e-23 91.7
Msa0515070 AT3G48040.1 35.758 165 92 5 11 163 10 172 4.52e-21 87.0
Msa0515070 AT4G35020.1 33.333 165 96 5 11 163 8 170 1.68e-20 85.5
Msa0515070 AT4G35020.2 33.333 165 96 5 11 163 8 170 1.68e-20 85.5
Msa0515070 AT4G35020.3 33.333 165 96 5 11 163 8 170 1.68e-20 85.5
Msa0515070 AT1G20090.1 32.727 165 97 4 11 163 7 169 6.67e-20 83.6
Msa0515070 AT1G75840.1 32.727 165 97 4 11 163 8 170 7.18e-20 83.6
Msa0515070 AT5G62880.1 34.545 165 94 5 11 163 10 172 7.47e-20 84.0
Msa0515070 AT3G51300.1 32.121 165 98 4 11 163 8 170 2.03e-19 82.4
Msa0515070 AT4G35950.1 32.727 165 97 4 11 163 8 170 2.05e-19 82.4
Msa0515070 AT2G17800.2 32.727 165 97 4 11 163 8 170 2.67e-19 82.0
Msa0515070 AT2G17800.1 32.727 165 97 4 11 163 8 170 2.67e-19 82.0
Msa0515070 AT3G48040.2 35.897 156 86 5 11 154 10 163 3.59e-19 81.3
Msa0515070 AT4G28950.1 32.515 163 98 5 11 163 8 168 1.79e-18 80.1
Msa0515070 AT5G62880.2 38.525 122 72 3 11 131 10 129 3.70e-18 79.0
Msa0515070 AT5G45970.1 30.909 165 100 5 11 163 8 170 4.12e-18 79.3
Msa0515070 AT4G35020.4 35.965 114 70 3 11 123 8 119 2.06e-15 70.5
Msa0515070 AT4G35020.5 35.965 114 70 3 11 123 8 119 2.06e-15 70.5
Msa0515070 AT5G46025.1 36.782 87 54 1 85 170 20 106 4.15e-15 68.9
Msa0515070 AT3G51290.2 31.818 132 77 4 44 163 641 771 2.46e-11 62.8

Find 53 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCACTTGACACAATTAT+TGG 0.192149 3_4:-66054865 Msa0515070:three_prime_UTR
TATTGGCTTGGCAACTAAAA+TGG 0.194288 3_4:-66054848 Msa0515070:three_prime_UTR
TAAGAAAACGAGTTTGTATT+TGG 0.198916 3_4:+66059366 None:intergenic
GAACGTGAATAACGCGAATC+TGG 0.310056 3_4:+66059447 None:intergenic
AAGATTGCTTCAGCCTTGCC+TGG 0.320177 3_4:-66055023 Msa0515070:CDS
CAGTGATGTTATTATCGTTT+TGG 0.337333 3_4:-66056191 Msa0515070:CDS
TCCACCTTGCTGCTCTGTCC+TGG 0.375622 3_4:+66054932 None:intergenic
CATTGGTACAAAAGTACGTT+TGG 0.379590 3_4:+66054804 None:intergenic
GAAGCAATCTTGCGAAATAA+AGG 0.385222 3_4:+66055035 None:intergenic
CAATGCTCTTCAGTTTAGTT+TGG 0.388867 3_4:-66054786 Msa0515070:three_prime_UTR
TACAAAAGTACGTTTGGGTT+TGG 0.416717 3_4:+66054810 None:intergenic
GAACAGTTCGTTTGCAGCTC+TGG 0.419041 3_4:-66057994 Msa0515070:intron
TAAATTCGACACTACCTATC+AGG 0.424038 3_4:-66059293 Msa0515070:intron
CTCTGTCCTGGATGAATTCA+CGG 0.424353 3_4:+66054944 None:intergenic
TAGTGTTAAGAAATGATTGC+CGG 0.427909 3_4:+66056248 None:intergenic
AGTACGTTTGGGTTTGGCCT+AGG 0.430088 3_4:+66054816 None:intergenic
AACGAACTGTTCGATCTTCA+AGG 0.432629 3_4:+66058005 None:intergenic
TGCTTCAGCCTTGCCTGGGA+TGG 0.434278 3_4:-66055018 Msa0515070:CDS
TCTACATTTCAGGCGACAAT+TGG 0.437446 3_4:-66058055 Msa0515070:intron
ACTTGACACAATTATTGGCT+TGG 0.447901 3_4:-66054860 Msa0515070:three_prime_UTR
GCTCTTCAGTTTAGTTTGGT+AGG 0.461266 3_4:-66054782 Msa0515070:three_prime_UTR
ATGCCAAGTCGCGTGAGAGC+GGG 0.477457 3_4:-66055392 Msa0515070:CDS
AACGTGAATAACGCGAATCT+GGG 0.495484 3_4:+66059448 None:intergenic
ATTGGTACAAAAGTACGTTT+GGG 0.496039 3_4:+66054805 None:intergenic
TTCTTAGGTGATCAATCCGT+TGG 0.500123 3_4:-66059349 Msa0515070:CDS
AGATTGCTTCAGCCTTGCCT+GGG 0.515055 3_4:-66055022 Msa0515070:CDS
GTTGAAAGACTTTCCATCCC+AGG 0.517172 3_4:+66055005 None:intergenic
GAAGAATCTCTTATATAACT+TGG 0.525048 3_4:+66057831 None:intergenic
TAACACTAACAAGTGGGTTG+AGG 0.526084 3_4:-66056233 Msa0515070:CDS
AGGACAGAGCAGCAAGGTGG+AGG 0.526096 3_4:-66054930 Msa0515070:CDS
ATAGAAACCAGTGCAAAAGC+AGG 0.533135 3_4:-66055360 Msa0515070:CDS
TAACTCCACAGGGATACTGC+AGG 0.545708 3_4:-66057879 Msa0515070:intron
TAATAGGCAAGTCTCTATAG+AGG 0.546908 3_4:-66055421 Msa0515070:intron
GATCCCGCTCTCACGCGACT+TGG 0.552486 3_4:+66055389 None:intergenic
CACTAACAAGTGGGTTGAGG+AGG 0.555833 3_4:-66056230 Msa0515070:CDS
ACGAGTTTGTATTTGGCTAG+AGG 0.566889 3_4:+66059373 None:intergenic
CAAACTAAACTGAAGAGCAT+TGG 0.570927 3_4:+66054787 None:intergenic
AAAAGCAGGATTTAATATCA+AGG 0.572165 3_4:-66055346 Msa0515070:intron
ATTTCTTAACACTAACAAGT+GGG 0.572336 3_4:-66056239 Msa0515070:CDS
TTCAACAAAGCAAGAAGACA+TGG 0.574137 3_4:-66054988 Msa0515070:CDS
AAGACTTTCCATCCCAGGCA+AGG 0.577100 3_4:+66055010 None:intergenic
AATCTACCGTGAATTCATCC+AGG 0.579097 3_4:-66054950 Msa0515070:CDS
CATTTCTTAACACTAACAAG+TGG 0.582798 3_4:-66056240 Msa0515070:CDS
AGGCAAGTCTCTATAGAGGA+AGG 0.591101 3_4:-66055417 Msa0515070:CDS
GATGCCAAGTCGCGTGAGAG+CGG 0.614649 3_4:-66055393 Msa0515070:CDS
AACTCCACAGGGATACTGCA+GGG 0.615234 3_4:-66057878 Msa0515070:intron
TAAAATGGAAATATGCACCT+AGG 0.622590 3_4:-66054833 Msa0515070:three_prime_UTR
TATAAGAGATTCTTCAGTCG+CGG 0.624663 3_4:-66057823 Msa0515070:CDS
TCCAGGACAGAGCAGCAAGG+TGG 0.660114 3_4:-66054933 Msa0515070:CDS
CATGAAGCGAGTGATGATGC+TGG 0.674864 3_4:+66059321 None:intergenic
GAGGAGGTACGTGCAGAACG+AGG 0.683803 3_4:-66056214 Msa0515070:CDS
TCATCCAGGACAGAGCAGCA+AGG 0.690459 3_4:-66054936 Msa0515070:CDS
AAACAGTGCTATTCACAACA+AGG 0.704518 3_4:-66054896 Msa0515070:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAGAAACAAAAA+TGG - chr3_4:66054816-66054835 Msa0515070:three_prime_UTR 10.0%
!! AATAAATTAGAAATGAAAAA+GGG + chr3_4:66054962-66054981 None:intergenic 10.0%
!! TAATTATTATACATTCTTTA+AGG - chr3_4:66057731-66057750 Msa0515070:intron 10.0%
!! ATAAATTAGAAATGAAAAAG+GGG + chr3_4:66054961-66054980 None:intergenic 15.0%
!! CAATAAATTAGAAATGAAAA+AGG + chr3_4:66054963-66054982 None:intergenic 15.0%
!! TTTAATACATATACAAACAA+AGG - chr3_4:66055471-66055490 Msa0515070:intron 15.0%
!!! ATAATATGTTGATTTTGATT+CGG - chr3_4:66055732-66055751 Msa0515070:intron 15.0%
!! TTCAAAATATGCAAAAAAAT+GGG + chr3_4:66056312-66056331 None:intergenic 15.0%
!! ATTCAAAATATGCAAAAAAA+TGG + chr3_4:66056313-66056332 None:intergenic 15.0%
!! CAAAAAAAATTAATACTTAC+TGG + chr3_4:66056444-66056463 None:intergenic 15.0%
!!! AATTTTTTTTTCAATCTATG+AGG - chr3_4:66057900-66057919 Msa0515070:intron 15.0%
!! CAATAAAACATTCACTAAAT+AGG + chr3_4:66055179-66055198 None:intergenic 20.0%
!!! TTTTATTGTGTATTAAGAAC+AGG - chr3_4:66055191-66055210 Msa0515070:intron 20.0%
!! TTTATTGTGTATTAAGAACA+GGG - chr3_4:66055192-66055211 Msa0515070:intron 20.0%
!! TACATCTAGTAATTTGAAAA+GGG + chr3_4:66055381-66055400 None:intergenic 20.0%
!! ATACATCTAGTAATTTGAAA+AGG + chr3_4:66055382-66055401 None:intergenic 20.0%
!! AAGTTAGTAGTATTAATTAG+GGG + chr3_4:66055428-66055447 None:intergenic 20.0%
!! CAAGTTAGTAGTATTAATTA+GGG + chr3_4:66055429-66055448 None:intergenic 20.0%
!! ACAAGTTAGTAGTATTAATT+AGG + chr3_4:66055430-66055449 None:intergenic 20.0%
!!! AATTGTAGGAATTATTTTCA+AGG - chr3_4:66055884-66055903 Msa0515070:intron 20.0%
!!! ATTGTAGGAATTATTTTCAA+GGG - chr3_4:66055885-66055904 Msa0515070:intron 20.0%
!! TTTAACGTTAGCAAATAATT+TGG + chr3_4:66056607-66056626 None:intergenic 20.0%
!!! TCATGAACTTATTTTAACTT+TGG + chr3_4:66056958-66056977 None:intergenic 20.0%
!!! GTTTTACATTAAAAGTACTT+CGG - chr3_4:66057047-66057066 Msa0515070:intron 20.0%
!!! CATTGTAATTTGTAAGTTTA+TGG + chr3_4:66057323-66057342 None:intergenic 20.0%
!! TTACAAATTACAATGTTGTA+TGG - chr3_4:66057328-66057347 Msa0515070:intron 20.0%
!! TACAAATTACAATGTTGTAT+GGG - chr3_4:66057329-66057348 Msa0515070:intron 20.0%
!!! TGTTTCGTTTTCAAAAAAAT+TGG - chr3_4:66058244-66058263 Msa0515070:intron 20.0%
!! GTTCTGTTTGAATATTATAA+TGG - chr3_4:66058290-66058309 Msa0515070:intron 20.0%
!! TGTTTGAATATTATAATGGT+AGG - chr3_4:66058294-66058313 Msa0515070:intron 20.0%
!! GAAATAATAACTTCAAAGTA+TGG + chr3_4:66058454-66058473 None:intergenic 20.0%
!! TATTTCAAAACCATATTTAG+AGG - chr3_4:66058468-66058487 Msa0515070:intron 20.0%
!! TATGAATTTACCTCTAAATA+TGG + chr3_4:66058481-66058500 None:intergenic 20.0%
!! ATATTTAGAGGTAAATTCAT+AGG - chr3_4:66058480-66058499 Msa0515070:intron 20.0%
!!! ATTTTGTTTTTTTCCTCATA+TGG - chr3_4:66058704-66058723 Msa0515070:intron 20.0%
! TAAGAAAACGAGTTTGTATT+TGG + chr3_4:66054860-66054879 None:intergenic 25.0%
!! AAATACAAACTCGTTTTCTT+AGG - chr3_4:66054859-66054878 Msa0515070:three_prime_UTR 25.0%
! GGACAAAAATAAAGTCAAAT+GGG + chr3_4:66055346-66055365 None:intergenic 25.0%
! ACATCTAGTAATTTGAAAAG+GGG + chr3_4:66055380-66055399 None:intergenic 25.0%
!! TATTAATTAGGGGTATGTTT+AGG + chr3_4:66055418-66055437 None:intergenic 25.0%
! TATACAAACAAAGGACAATA+TGG - chr3_4:66055480-66055499 Msa0515070:intron 25.0%
! AAGAAATGTGAAAAAAGCAA+AGG - chr3_4:66055573-66055592 Msa0515070:intron 25.0%
! AGAAATGTGAAAAAAGCAAA+GGG - chr3_4:66055574-66055593 Msa0515070:intron 25.0%
!! ATTTTATTCTGCATTGTCTT+TGG - chr3_4:66055665-66055684 Msa0515070:intron 25.0%
!!! TTGTAGGAATTATTTTCAAG+GGG - chr3_4:66055886-66055905 Msa0515070:intron 25.0%
! GAAGAATCTCTTATATAACT+TGG + chr3_4:66056395-66056414 None:intergenic 25.0%
! CAAAAAAAGTTAATACTGAC+TGG + chr3_4:66056510-66056529 None:intergenic 25.0%
! AAGAAAACAATACAATAGCA+TGG - chr3_4:66056537-66056556 Msa0515070:intron 25.0%
! AAACTAAATGACCGATATAT+AGG - chr3_4:66057186-66057205 Msa0515070:intron 25.0%
!! TAAGCTTAAACGAAGATTTT+AGG - chr3_4:66057291-66057310 Msa0515070:intron 25.0%
!! AAGCTTAAACGAAGATTTTA+GGG - chr3_4:66057292-66057311 Msa0515070:intron 25.0%
! AGGAACAAAAATACATTTCT+TGG - chr3_4:66057447-66057466 Msa0515070:intron 25.0%
! ATCTCTAAATCTCATTAGTT+TGG + chr3_4:66057562-66057581 None:intergenic 25.0%
! TGATATCATAATTTCTGTCA+TGG + chr3_4:66057610-66057629 None:intergenic 25.0%
! ATTTCTTAACACTAACAAGT+GGG - chr3_4:66057984-66058003 Msa0515070:intron 25.0%
! AAACTGATCTTGTTGAAAAA+AGG - chr3_4:66058064-66058083 Msa0515070:intron 25.0%
! GAATTATAGCAAACATAGTA+TGG + chr3_4:66058223-66058242 None:intergenic 25.0%
! GTAATACTAAAAATTCGTAC+AGG - chr3_4:66058415-66058434 Msa0515070:intron 25.0%
! TTGCTATAAGAAGAATTGTA+TGG + chr3_4:66058737-66058756 None:intergenic 25.0%
! AAGCATCATAATGTCATTAT+AGG + chr3_4:66058782-66058801 None:intergenic 25.0%
! TGACATTATGATGCTTTAAT+AGG - chr3_4:66058786-66058805 Msa0515070:intron 25.0%
! AAAAGCAGGATTTAATATCA+AGG - chr3_4:66058877-66058896 Msa0515070:intron 25.0%
! TACATCATACATGAACATAT+GGG + chr3_4:66058930-66058949 None:intergenic 25.0%
! ATACATCATACATGAACATA+TGG + chr3_4:66058931-66058950 None:intergenic 25.0%
! ATTATTTTCCTGTTGCGTAT+AGG - chr3_4:66055246-66055265 Msa0515070:intron 30.0%
CAAAAATAAAGTCAAATGGG+TGG + chr3_4:66055343-66055362 None:intergenic 30.0%
GGGACAAAAATAAAGTCAAA+TGG + chr3_4:66055347-66055366 None:intergenic 30.0%
TGAAAAGGGGTTTAGATTTA+GGG + chr3_4:66055367-66055386 None:intergenic 30.0%
TTGAAAAGGGGTTTAGATTT+AGG + chr3_4:66055368-66055387 None:intergenic 30.0%
TTAGGACTTGATTAGGAATT+AGG - chr3_4:66055629-66055648 Msa0515070:intron 30.0%
! TTTGGAGTATTATGAACAGA+GGG - chr3_4:66055683-66055702 Msa0515070:intron 30.0%
AGGGAATAGATCTAAATTCA+AGG - chr3_4:66055702-66055721 Msa0515070:intron 30.0%
! GAGCTTTTATTAGCAATTGT+AGG - chr3_4:66055870-66055889 Msa0515070:intron 30.0%
! GATAAGTATTGTAACTGATG+CGG - chr3_4:66055969-66055988 Msa0515070:intron 30.0%
TATTACCAACTTATCATGCA+AGG - chr3_4:66056037-66056056 Msa0515070:intron 30.0%
! TCTTCTTTGTCATGTTGTTT+CGG - chr3_4:66056080-66056099 Msa0515070:intron 30.0%
AAGATACTAACAGTAGGAAA+GGG + chr3_4:66056131-66056150 None:intergenic 30.0%
TTTGTCAAGATACTAACAGT+AGG + chr3_4:66056137-66056156 None:intergenic 30.0%
TTTGTAATCGTCTACATTTC+AGG - chr3_4:66056158-66056177 Msa0515070:intron 30.0%
TTGTAGTAGATTTCACATTG+TGG - chr3_4:66056270-66056289 Msa0515070:intron 30.0%
!!! TAGTCTATTTTAACTCCACA+GGG - chr3_4:66056334-66056353 Msa0515070:intron 30.0%
TTTGTTCTTAAGAGATTCTC+CGG - chr3_4:66056460-66056479 Msa0515070:intron 30.0%
CATAACTAAAAACCATATGC+TGG + chr3_4:66056680-66056699 None:intergenic 30.0%
!!! CAGCATATGGTTTTTAGTTA+TGG - chr3_4:66056678-66056697 Msa0515070:intron 30.0%
!!! TCTTTGTTTTTCTCTCAACT+TGG + chr3_4:66056708-66056727 None:intergenic 30.0%
! GAATTAACAAAGTTGACTCT+TGG + chr3_4:66056793-66056812 None:intergenic 30.0%
AGAGTCAACTTTGTTAATTC+TGG - chr3_4:66056793-66056812 Msa0515070:intron 30.0%
! ATTGTGTTCTTCTTTTTGTG+TGG - chr3_4:66056850-66056869 Msa0515070:intron 30.0%
! AAAGTAATGGAAGTTTGTGA+TGG - chr3_4:66056904-66056923 Msa0515070:intron 30.0%
!! TTAGCTTTAGAGGTGAAAAT+TGG - chr3_4:66057005-66057024 Msa0515070:intron 30.0%
!! ATATCGGTCATTTAGTTTGA+TGG + chr3_4:66057184-66057203 None:intergenic 30.0%
AAGTGGTAGTTTATATCACA+GGG + chr3_4:66057272-66057291 None:intergenic 30.0%
TAAGTGGTAGTTTATATCAC+AGG + chr3_4:66057273-66057292 None:intergenic 30.0%
AATCTTCGTTTAAGCTTAAG+TGG + chr3_4:66057289-66057308 None:intergenic 30.0%
AAGAATGTTACAACGATAAG+TGG - chr3_4:66057407-66057426 Msa0515070:intron 30.0%
TGGTTTACACAAGATAAGAT+AGG - chr3_4:66057427-66057446 Msa0515070:intron 30.0%
CTATACTCCATTTAGTCAAT+CGG + chr3_4:66057505-66057524 None:intergenic 30.0%
GGCGAGTTAAGAAAAATTAT+AGG - chr3_4:66057536-66057555 Msa0515070:intron 30.0%
CACTGAATAATTGAAAACGT+CGG - chr3_4:66057797-66057816 Msa0515070:intron 30.0%
AATAGAAAACACACACAAAG+AGG + chr3_4:66057837-66057856 None:intergenic 30.0%
!! CAGCTTGCTAAGAAATTTAA+TGG + chr3_4:66057943-66057962 None:intergenic 30.0%
TAGTGTTAAGAAATGATTGC+CGG + chr3_4:66057978-66057997 None:intergenic 30.0%
CATTTCTTAACACTAACAAG+TGG - chr3_4:66057983-66058002 Msa0515070:intron 30.0%
! CAGTGATGTTATTATCGTTT+TGG - chr3_4:66058032-66058051 Msa0515070:CDS 30.0%
!! GATGTTATTATCGTTTTGGT+TGG - chr3_4:66058036-66058055 Msa0515070:CDS 30.0%
TTATTGCCTAACAACTGAAT+TGG - chr3_4:66058332-66058351 Msa0515070:intron 30.0%
ACTTAACCAATTCAGTTGTT+AGG + chr3_4:66058341-66058360 None:intergenic 30.0%
! ACTTCAAAGTATGGCAATTT+TGG + chr3_4:66058445-66058464 None:intergenic 30.0%
CATATCCAACAATATCATCA+TGG + chr3_4:66058507-66058526 None:intergenic 30.0%
ACATCATACATGAACATATG+GGG + chr3_4:66058929-66058948 None:intergenic 30.0%
TGTTCACTTGACACAATTAT+TGG - chr3_4:66059358-66059377 Msa0515070:CDS 30.0%
TAAAATGGAAATATGCACCT+AGG - chr3_4:66059390-66059409 Msa0515070:CDS 30.0%
! ATTGGTACAAAAGTACGTTT+GGG + chr3_4:66059421-66059440 None:intergenic 30.0%
TAAATTCGACACTACCTATC+AGG - chr3_4:66054930-66054949 Msa0515070:CDS 35.0%
AAAAAGGGGAATAACCTGAT+AGG + chr3_4:66054947-66054966 None:intergenic 35.0%
! GATATTTGTGTGATTTTGCG+AGG - chr3_4:66055097-66055116 Msa0515070:intron 35.0%
TTGTGTATTAAGAACAGGGA+TGG - chr3_4:66055196-66055215 Msa0515070:intron 35.0%
CAAATGGGTGGTTAGATATA+GGG + chr3_4:66055331-66055350 None:intergenic 35.0%
TCAAATGGGTGGTTAGATAT+AGG + chr3_4:66055332-66055351 None:intergenic 35.0%
AATGTGAAAAAAGCAAAGGG+TGG - chr3_4:66055577-66055596 Msa0515070:intron 35.0%
TACGTGATTAGGACTTGATT+AGG - chr3_4:66055622-66055641 Msa0515070:intron 35.0%
ATGCAGAATAAAATATCCGC+TGG + chr3_4:66055659-66055678 None:intergenic 35.0%
!! CTTTGGAGTATTATGAACAG+AGG - chr3_4:66055682-66055701 Msa0515070:intron 35.0%
CACAAACAAGATACGAGTAT+TGG - chr3_4:66055947-66055966 Msa0515070:intron 35.0%
AGTAACCTTGCATGATAAGT+TGG + chr3_4:66056045-66056064 None:intergenic 35.0%
AGATACTAACAGTAGGAAAG+GGG + chr3_4:66056130-66056149 None:intergenic 35.0%
CAAGATACTAACAGTAGGAA+AGG + chr3_4:66056132-66056151 None:intergenic 35.0%
!!! GTAGTCTATTTTAACTCCAC+AGG - chr3_4:66056333-66056352 Msa0515070:intron 35.0%
TATAAGAGATTCTTCAGTCG+CGG - chr3_4:66056400-66056419 Msa0515070:intron 35.0%
TCATATACAATAACCGCAAC+CGG + chr3_4:66056482-66056501 None:intergenic 35.0%
ACTAAAAACCATATGCTGGA+TGG + chr3_4:66056676-66056695 None:intergenic 35.0%
AACACAATGACATTGTCTCT+TGG + chr3_4:66056838-66056857 None:intergenic 35.0%
GTGGATAATATAGTCCTAGT+TGG - chr3_4:66056869-66056888 Msa0515070:intron 35.0%
TGAAGTACAAGTTTAGCAAG+AGG - chr3_4:66056975-66056994 Msa0515070:intron 35.0%
ATAGAGAATAGAGAGAGACA+TGG + chr3_4:66057151-66057170 None:intergenic 35.0%
!! ATTTAGTTTGATGGAGCTGT+CGG + chr3_4:66057175-66057194 None:intergenic 35.0%
AGTTGGCATCTCCTATATAT+CGG + chr3_4:66057200-66057219 None:intergenic 35.0%
ATATAGGAGATGCCAACTAT+AGG - chr3_4:66057202-66057221 Msa0515070:intron 35.0%
GAGCCAAAAAAAAGATGTCT+CGG - chr3_4:66057372-66057391 Msa0515070:intron 35.0%
!!! ACACCGAGACATCTTTTTTT+TGG + chr3_4:66057378-66057397 None:intergenic 35.0%
!! AAAAGCACCGATTGACTAAA+TGG - chr3_4:66057495-66057514 Msa0515070:intron 35.0%
ATGGAGTATAGTCCAACATA+AGG - chr3_4:66057514-66057533 Msa0515070:intron 35.0%
TGGAGTATAGTCCAACATAA+GGG - chr3_4:66057515-66057534 Msa0515070:intron 35.0%
CATGGATCAAGTGTTAAGAT+AGG + chr3_4:66057592-66057611 None:intergenic 35.0%
AACAGAAATAACAGCAAAGC+AGG + chr3_4:66057664-66057683 None:intergenic 35.0%
TTGTTGAAAAAAGGTGAGCA+GGG - chr3_4:66058073-66058092 Msa0515070:intron 35.0%
!! CAGTTGATAGGTAACTATCA+AGG + chr3_4:66058128-66058147 None:intergenic 35.0%
! CTTGATAGTTACCTATCAAC+TGG - chr3_4:66058126-66058145 Msa0515070:intron 35.0%
TAGGTCCATGATGATATTGT+TGG - chr3_4:66058499-66058518 Msa0515070:intron 35.0%
TGTAAGAGTAGCTGATTGTA+AGG - chr3_4:66058636-66058655 Msa0515070:intron 35.0%
TAATAGGCAAGTCTCTATAG+AGG - chr3_4:66058802-66058821 Msa0515070:intron 35.0%
!! ATTAAATCCTGCTTTTGCAC+TGG + chr3_4:66058873-66058892 None:intergenic 35.0%
AGACAGACTTCAGAATTCAA+AGG + chr3_4:66058972-66058991 None:intergenic 35.0%
! ATCTAAAAGTATGCCATCCA+TGG - chr3_4:66059049-66059068 Msa0515070:intron 35.0%
! ACAAGTTTGAAATCCATGGA+TGG + chr3_4:66059065-66059084 None:intergenic 35.0%
! GACAACAAGTTTGAAATCCA+TGG + chr3_4:66059069-66059088 None:intergenic 35.0%
!! CTGCAACATTTTAATCACTC+GGG + chr3_4:66059168-66059187 None:intergenic 35.0%
!! GCTGCAACATTTTAATCACT+CGG + chr3_4:66059169-66059188 None:intergenic 35.0%
GAAGCAATCTTGCGAAATAA+AGG + chr3_4:66059191-66059210 None:intergenic 35.0%
TTCAACAAAGCAAGAAGACA+TGG - chr3_4:66059235-66059254 Msa0515070:intron 35.0%
! AAACAGTGCTATTCACAACA+AGG - chr3_4:66059327-66059346 Msa0515070:CDS 35.0%
! ACTTGACACAATTATTGGCT+TGG - chr3_4:66059363-66059382 Msa0515070:CDS 35.0%
!! TATTGGCTTGGCAACTAAAA+TGG - chr3_4:66059375-66059394 Msa0515070:CDS 35.0%
! TACAAAAGTACGTTTGGGTT+TGG + chr3_4:66059416-66059435 None:intergenic 35.0%
! CATTGGTACAAAAGTACGTT+TGG + chr3_4:66059422-66059441 None:intergenic 35.0%
! CAAACTAAACTGAAGAGCAT+TGG + chr3_4:66059439-66059458 None:intergenic 35.0%
CAATGCTCTTCAGTTTAGTT+TGG - chr3_4:66059437-66059456 Msa0515070:five_prime_UTR 35.0%
AACGTGAATAACGCGAATCT+GGG + chr3_4:66054778-66054797 None:intergenic 40.0%
ACGAGTTTGTATTTGGCTAG+AGG + chr3_4:66054853-66054872 None:intergenic 40.0%
TTCTTAGGTGATCAATCCGT+TGG - chr3_4:66054874-66054893 Msa0515070:three_prime_UTR 40.0%
!!! GATGATGCTGGTTTTACCAA+CGG + chr3_4:66054893-66054912 None:intergenic 40.0%
! TTGTGTGATTTTGCGAGGAT+CGG - chr3_4:66055102-66055121 Msa0515070:intron 40.0%
AGCAAAGGGTGGTTACATTA+AGG - chr3_4:66055588-66055607 Msa0515070:intron 40.0%
GCAAAGGGTGGTTACATTAA+GGG - chr3_4:66055589-66055608 Msa0515070:intron 40.0%
GGACTTGATTAGGAATTAGG+AGG - chr3_4:66055632-66055651 Msa0515070:intron 40.0%
GGTGCGAAATTGTTTCGATT+AGG - chr3_4:66055753-66055772 Msa0515070:intron 40.0%
AATGCACGAGTAAAGCAAGA+AGG + chr3_4:66055803-66055822 None:intergenic 40.0%
!! CGTTAAAGAAGTGCTAGAGT+AGG - chr3_4:66055842-66055861 Msa0515070:intron 40.0%
TTGTGCTTCGTCGACAAAAA+TGG + chr3_4:66055932-66055951 None:intergenic 40.0%
GATACTAACAGTAGGAAAGG+GGG + chr3_4:66056129-66056148 None:intergenic 40.0%
TCTACATTTCAGGCGACAAT+TGG - chr3_4:66056168-66056187 Msa0515070:CDS 40.0%
AACGAACTGTTCGATCTTCA+AGG + chr3_4:66056221-66056240 None:intergenic 40.0%
AACTCTCTTCTGATAGGACA+TGG + chr3_4:66056650-66056669 None:intergenic 40.0%
TGGTCAAACTCTCTTCTGAT+AGG + chr3_4:66056656-66056675 None:intergenic 40.0%
ATATAGTCCTAGTTGGAGAC+TGG - chr3_4:66056876-66056895 Msa0515070:intron 40.0%
GAGACTGGAGAAGAAAGTAA+TGG - chr3_4:66056891-66056910 Msa0515070:intron 40.0%
! GTTTGTGATGGAGTCAGTAT+TGG - chr3_4:66056916-66056935 Msa0515070:intron 40.0%
! GAGTCAGTATTGGAAACACA+AGG - chr3_4:66056926-66056945 Msa0515070:intron 40.0%
GAATTGAGAGAGATCTGATG+AGG - chr3_4:66057122-66057141 Msa0515070:intron 40.0%
CCAACTATAGGATCCAAGTA+GGG - chr3_4:66057214-66057233 Msa0515070:intron 40.0%
! TCTATTTTGTCCACCCTACT+TGG + chr3_4:66057230-66057249 None:intergenic 40.0%
AAGTAGGGTGGACAAAATAG+AGG - chr3_4:66057229-66057248 Msa0515070:intron 40.0%
TTCTTAACTCGCCCTTATGT+TGG + chr3_4:66057529-66057548 None:intergenic 40.0%
ACACAAAGAGGTAGAGAACA+GGG + chr3_4:66057825-66057844 None:intergenic 40.0%
!! TAACACTAACAAGTGGGTTG+AGG - chr3_4:66057990-66058009 Msa0515070:intron 40.0%
CTTGTTGAAAAAAGGTGAGC+AGG - chr3_4:66058072-66058091 Msa0515070:intron 40.0%
AAAATGCTCAATGTCCTGAC+TGG + chr3_4:66058105-66058124 None:intergenic 40.0%
AAGTCAGCAAACCAGTTGAT+AGG + chr3_4:66058140-66058159 None:intergenic 40.0%
ACATCACATGCAAAGCTAAC+TGG + chr3_4:66058533-66058552 None:intergenic 40.0%
GTATGGATATGTGCCATATG+AGG + chr3_4:66058720-66058739 None:intergenic 40.0%
ATAGAAACCAGTGCAAAAGC+AGG - chr3_4:66058863-66058882 Msa0515070:intron 40.0%
ATGCAGCTGAGAATCATTCA+AGG + chr3_4:66059020-66059039 None:intergenic 40.0%
AATCTACCGTGAATTCATCC+AGG - chr3_4:66059273-66059292 Msa0515070:intron 40.0%
!! GCTCTTCAGTTTAGTTTGGT+AGG - chr3_4:66059441-66059460 Msa0515070:five_prime_UTR 40.0%
GAACGTGAATAACGCGAATC+TGG + chr3_4:66054779-66054798 None:intergenic 45.0%
GCTTGTTTCCTATACGCAAC+AGG + chr3_4:66055257-66055276 None:intergenic 45.0%
TGGTTAGATATAGGGACGGA+GGG + chr3_4:66055323-66055342 None:intergenic 45.0%
TGGGTGGTTAGATATAGGGA+CGG + chr3_4:66055327-66055346 None:intergenic 45.0%
AGGGTGGTTACATTAAGGGA+CGG - chr3_4:66055593-66055612 Msa0515070:intron 45.0%
TGGTTACATTAAGGGACGGA+GGG - chr3_4:66055597-66055616 Msa0515070:intron 45.0%
TTAGGAATTAGGAGGACCAG+CGG - chr3_4:66055640-66055659 Msa0515070:intron 45.0%
CTTAAGAGATTCTCCGGTTG+CGG - chr3_4:66056466-66056485 Msa0515070:intron 45.0%
GAGTTTGACCATCCAGCATA+TGG - chr3_4:66056665-66056684 Msa0515070:intron 45.0%
TTCTTCTCCAGTCTCCAACT+AGG + chr3_4:66056886-66056905 None:intergenic 45.0%
!! AGGCACGAGTTTAGCTTTAG+AGG - chr3_4:66056995-66057014 Msa0515070:intron 45.0%
GCCAACTATAGGATCCAAGT+AGG - chr3_4:66057213-66057232 Msa0515070:intron 45.0%
CCCTACTTGGATCCTATAGT+TGG + chr3_4:66057217-66057236 None:intergenic 45.0%
ACTATAGGATCCAAGTAGGG+TGG - chr3_4:66057217-66057236 Msa0515070:intron 45.0%
TTCTTGGAAGATAGCACGTG+TGG - chr3_4:66057463-66057482 Msa0515070:intron 45.0%
TCTATGTACTCCCCAGCTAT+TGG - chr3_4:66057635-66057654 Msa0515070:intron 45.0%
AAAGCAGGCTTCCAATAGCT+GGG + chr3_4:66057649-66057668 None:intergenic 45.0%
CACACAAAGAGGTAGAGAAC+AGG + chr3_4:66057826-66057845 None:intergenic 45.0%
AGGCAAGTCTCTATAGAGGA+AGG - chr3_4:66058806-66058825 Msa0515070:intron 45.0%
CATGAACATATGGGGTGACT+CGG + chr3_4:66058921-66058940 None:intergenic 45.0%
GTTGAAAGACTTTCCATCCC+AGG + chr3_4:66059221-66059240 None:intergenic 45.0%
CTCTGTCCTGGATGAATTCA+CGG + chr3_4:66059282-66059301 None:intergenic 45.0%
! CATGAAGCGAGTGATGATGC+TGG + chr3_4:66054905-66054924 None:intergenic 50.0%
GTGGTTAGATATAGGGACGG+AGG + chr3_4:66055324-66055343 None:intergenic 50.0%
GTGGTTACATTAAGGGACGG+AGG - chr3_4:66055596-66055615 Msa0515070:intron 50.0%
ACTGATGCGGCGATACGTTA+AGG - chr3_4:66055982-66056001 Msa0515070:intron 50.0%
GAACAGTTCGTTTGCAGCTC+TGG - chr3_4:66056229-66056248 Msa0515070:CDS 50.0%
TAACTCCACAGGGATACTGC+AGG - chr3_4:66056344-66056363 Msa0515070:intron 50.0%
AACTCCACAGGGATACTGCA+GGG - chr3_4:66056345-66056364 Msa0515070:intron 50.0%
AAGCAGGCTTCCAATAGCTG+GGG + chr3_4:66057648-66057667 None:intergenic 50.0%
CAAAGCAGGCTTCCAATAGC+TGG + chr3_4:66057650-66057669 None:intergenic 50.0%
CAAGCTGTTCTGATGCAGAC+CGG - chr3_4:66057956-66057975 Msa0515070:intron 50.0%
!! CACTAACAAGTGGGTTGAGG+AGG - chr3_4:66057993-66058012 Msa0515070:intron 50.0%
AAGATTGCTTCAGCCTTGCC+TGG - chr3_4:66059200-66059219 Msa0515070:intron 50.0%
AGATTGCTTCAGCCTTGCCT+GGG - chr3_4:66059201-66059220 Msa0515070:intron 50.0%
AAGACTTTCCATCCCAGGCA+AGG + chr3_4:66059216-66059235 None:intergenic 50.0%
! AGTACGTTTGGGTTTGGCCT+AGG + chr3_4:66059410-66059429 None:intergenic 50.0%
! GACGGAGGGAGTACGTGATT+AGG - chr3_4:66055611-66055630 Msa0515070:intron 55.0%
TCATCCAGGACAGAGCAGCA+AGG - chr3_4:66059287-66059306 Msa0515070:intron 55.0%
CTTGCCCTGCAGTATCCCTG+TGG + chr3_4:66056352-66056371 None:intergenic 60.0%
! GAGGAGGTACGTGCAGAACG+AGG - chr3_4:66058009-66058028 Msa0515070:CDS 60.0%
GAGCAGGGAGTCAACCAGTC+AGG - chr3_4:66058088-66058107 Msa0515070:intron 60.0%
GATGCCAAGTCGCGTGAGAG+CGG - chr3_4:66058830-66058849 Msa0515070:intron 60.0%
ATGCCAAGTCGCGTGAGAGC+GGG - chr3_4:66058831-66058850 Msa0515070:intron 60.0%
TGCTTCAGCCTTGCCTGGGA+TGG - chr3_4:66059205-66059224 Msa0515070:intron 60.0%
TCCAGGACAGAGCAGCAAGG+TGG - chr3_4:66059290-66059309 Msa0515070:intron 60.0%
TCCACCTTGCTGCTCTGTCC+TGG + chr3_4:66059294-66059313 None:intergenic 60.0%
AGGACAGAGCAGCAAGGTGG+AGG - chr3_4:66059293-66059312 Msa0515070:intron 60.0%
GATCCCGCTCTCACGCGACT+TGG + chr3_4:66058837-66058856 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 66054723 66059522 66054723 ID=Msa0515070;Name=Msa0515070
chr3_4 mRNA 66054723 66059522 66054723 ID=Msa0515070-mRNA-1;Parent=Msa0515070;Name=Msa0515070-mRNA-1;_AED=0.07;_eAED=0.07;_QI=112|0.8|0.66|1|1|1|6|194|206
chr3_4 exon 66054723 66055057 66054723 ID=Msa0515070-mRNA-1:exon:16990;Parent=Msa0515070-mRNA-1
chr3_4 exon 66055347 66055437 66055347 ID=Msa0515070-mRNA-1:exon:16989;Parent=Msa0515070-mRNA-1
chr3_4 exon 66056160 66056271 66056160 ID=Msa0515070-mRNA-1:exon:16988;Parent=Msa0515070-mRNA-1
chr3_4 exon 66057802 66057890 66057802 ID=Msa0515070-mRNA-1:exon:16987;Parent=Msa0515070-mRNA-1
chr3_4 exon 66057995 66058065 66057995 ID=Msa0515070-mRNA-1:exon:16986;Parent=Msa0515070-mRNA-1
chr3_4 exon 66059294 66059522 66059294 ID=Msa0515070-mRNA-1:exon:16985;Parent=Msa0515070-mRNA-1
chr3_4 five_prime_UTR 66059411 66059522 66059411 ID=Msa0515070-mRNA-1:five_prime_utr;Parent=Msa0515070-mRNA-1
chr3_4 CDS 66059294 66059410 66059294 ID=Msa0515070-mRNA-1:cds;Parent=Msa0515070-mRNA-1
chr3_4 CDS 66057995 66058065 66057995 ID=Msa0515070-mRNA-1:cds;Parent=Msa0515070-mRNA-1
chr3_4 CDS 66057802 66057890 66057802 ID=Msa0515070-mRNA-1:cds;Parent=Msa0515070-mRNA-1
chr3_4 CDS 66056160 66056271 66056160 ID=Msa0515070-mRNA-1:cds;Parent=Msa0515070-mRNA-1
chr3_4 CDS 66055347 66055437 66055347 ID=Msa0515070-mRNA-1:cds;Parent=Msa0515070-mRNA-1
chr3_4 CDS 66054917 66055057 66054917 ID=Msa0515070-mRNA-1:cds;Parent=Msa0515070-mRNA-1
chr3_4 three_prime_UTR 66054723 66054916 66054723 ID=Msa0515070-mRNA-1:three_prime_utr;Parent=Msa0515070-mRNA-1
Gene Sequence

>Msa0515070

ATGGTGACTGTATCACCTCTAGCCAAATACAAACTCGTTTTCTTAGGTGATCAATCCGTTGGTAAAACCAGCATCATCACTCGCTTCATGTATGATAAATTCGACACTACCTATCAGGCGACAATTGGTATTGACTTTTTGTCGAAAACAATGTACCTTGAAGATCGAACAGTTCGTTTGCAGCTCTGGGATACTGCAGGGCAAGAAAGATTTAGAAGTCTCATTCCAAGTTATATAAGAGATTCTTCAGTCGCGGTTATTGTGTATGATGTTGCCAACCGGCAATCATTTCTTAACACTAACAAGTGGGTTGAGGAGGTACGTGCAGAACGAGGCAGTGATGTTATTATCGTTTTGGTTGGAAACAAAACTGATCTTGTTGAAAAAAGGCAAGTCTCTATAGAGGAAGGAGATGCCAAGTCGCGTGAGAGCGGGATCATGTTTATAGAAACCAGTGCAAAAGCAGGATTTAATATCAAGCCTTTATTTCGCAAGATTGCTTCAGCCTTGCCTGGGATGGAAAGTCTTTCAACAAAGCAAGAAGACATGGTTGATGTAAATTTAAAATCTACCGTGAATTCATCCAGGACAGAGCAGCAAGGTGGAGGTTGTTCGTGCTAA
Protein sequence

>Msa0515070

MVTVSPLAKYKLVFLGDQSVGKTSIITRFMYDKFDTTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSSVAVIVYDVANRQSFLNTNKWVEEVRAERGSDVIIVLVGNKTDLVEKRQVSIEEGDAKSRESGIMFIETSAKAGFNIKPLFRKIASALPGMESLSTKQEDMVDVNLKSTVNSSRTEQQGGGCSC*