AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0430860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0430860 MtrunA17_Chr3g0134761 95.601 341 15 0 1 341 1 341 0.0 657
Msa0430860 MtrunA17_Chr1g0152561 76.737 331 70 4 5 334 16 340 3.71e-178 497
Msa0430860 MtrunA17_Chr2g0290151 49.020 306 140 3 44 337 156 457 2.62e-83 259
Msa0430860 MtrunA17_Chr1g0183781 49.477 287 140 2 51 337 440 721 5.19e-80 258
Msa0430860 MtrunA17_Chr3g0106931 51.748 286 133 2 51 336 553 833 1.96e-77 253
Msa0430860 MtrunA17_Chr7g0261371 46.809 282 145 2 51 332 514 790 1.90e-70 234
Msa0430860 MtrunA17_Chr4g0044411 45.357 280 149 1 51 330 373 648 1.58e-67 223
Msa0430860 MtrunA17_Chr4g0031431 43.262 282 156 1 51 332 353 630 2.00e-61 207
Msa0430860 MtrunA17_Chr7g0248251 40.794 277 160 1 54 330 370 642 2.52e-61 207
Msa0430860 MtrunA17_Chr1g0199321 45.714 280 148 1 51 330 345 620 1.65e-60 204
Msa0430860 MtrunA17_Chr7g0237931 41.581 291 155 4 43 328 325 605 1.28e-58 199
Msa0430860 MtrunA17_Chr5g0437411 39.655 290 162 6 54 334 408 693 7.00e-47 168
Msa0430860 MtrunA17_Chr5g0429231 35.379 277 170 5 58 329 41 313 6.12e-38 138
Msa0430860 MtrunA17_Chr7g0275081 36.170 282 168 7 53 326 171 448 8.79e-35 132
Msa0430860 MtrunA17_Chr2g0284461 37.329 292 169 9 54 339 399 682 1.31e-33 131
Msa0430860 MtrunA17_Chr7g0234671 32.616 279 183 4 55 330 175 451 2.24e-31 122
Msa0430860 MtrunA17_Chr5g0435041 33.803 284 177 6 51 329 20 297 4.96e-29 115
Msa0430860 MtrunA17_Chr1g0189811 34.375 288 183 5 51 334 157 442 8.84e-27 110
Msa0430860 MtrunA17_Chr3g0118001 26.882 279 195 3 54 329 214 486 8.75e-26 107
Msa0430860 MtrunA17_Chr6g0449461 30.479 292 184 8 52 333 3 285 2.92e-25 103
Msa0430860 MtrunA17_Chr3g0111451 34.708 291 176 7 51 333 41 325 1.77e-24 102
Msa0430860 MtrunA17_Chr8g0377151 31.985 272 178 6 72 338 449 718 6.37e-24 102
Msa0430860 MtrunA17_Chr4g0013091 30.690 290 185 8 50 330 416 698 1.11e-20 93.2
Msa0430860 MtrunA17_Chr4g0059201 29.412 306 198 10 42 339 417 712 7.16e-20 90.9
Msa0430860 MtrunA17_Chr6g0451581 25.822 213 153 2 116 325 299 509 1.17e-17 84.0
Msa0430860 MtrunA17_Chr1g0149941 27.931 290 190 9 53 329 175 458 1.74e-17 83.6
Msa0430860 MtrunA17_Chr3g0122721 28.947 266 181 6 72 332 448 710 2.33e-17 83.2
Msa0430860 MtrunA17_Chr3g0137061 27.491 291 193 7 51 329 110 394 4.72e-17 82.0
Msa0430860 MtrunA17_Chr3g0117851 27.586 290 160 8 47 330 378 623 7.25e-16 78.6
Msa0430860 MtrunA17_Chr4g0048811 30.882 204 134 6 129 329 121 320 1.85e-15 76.3
Msa0430860 MtrunA17_Chr1g0199031 39.496 119 55 1 132 250 18 119 2.40e-15 72.0
Msa0430860 MtrunA17_Chr8g0350681 28.251 223 146 7 91 309 238 450 8.49e-15 75.5
Msa0430860 MtrunA17_Chr2g0280671 29.008 262 166 9 75 329 131 379 2.67e-13 70.1
Msa0430860 MtrunA17_Chr7g0259851 25.214 234 163 3 58 291 276 497 3.56e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0430860 AT3G01400.1 73.824 340 78 7 5 341 21 352 1.13e-170 479
Msa0430860 AT5G58680.1 69.277 332 91 6 4 332 18 341 1.25e-158 448
Msa0430860 AT4G16490.1 50.505 297 141 2 38 332 172 464 1.10e-83 261
Msa0430860 AT2G23140.3 45.181 332 169 4 13 336 308 634 7.91e-81 258
Msa0430860 AT2G23140.5 45.181 332 169 4 13 336 308 634 7.91e-81 258
Msa0430860 AT2G23140.2 45.181 332 169 4 13 336 497 823 3.63e-79 258
Msa0430860 AT2G23140.4 45.181 332 169 4 13 336 497 823 3.63e-79 258
Msa0430860 AT2G23140.1 45.181 332 169 4 13 336 500 826 4.17e-79 258
Msa0430860 AT5G67340.1 48.252 286 142 3 51 335 423 703 9.41e-74 241
Msa0430860 AT5G67340.2 48.252 286 142 3 51 335 423 703 9.41e-74 241
Msa0430860 AT3G54790.2 48.057 283 141 3 53 334 440 717 3.77e-70 232
Msa0430860 AT3G54790.3 48.057 283 141 3 53 334 476 753 1.35e-69 231
Msa0430860 AT3G54790.1 48.057 283 141 3 53 334 476 753 1.35e-69 231
Msa0430860 AT1G23030.1 45.035 282 150 2 50 330 332 609 1.08e-68 226
Msa0430860 AT1G71020.2 45.907 281 148 2 51 330 195 472 1.21e-68 223
Msa0430860 AT1G71020.1 45.907 281 148 2 51 330 343 620 1.54e-67 223
Msa0430860 AT3G54850.1 47.518 282 142 3 50 330 346 622 1.11e-62 211
Msa0430860 AT3G46510.1 42.391 276 155 1 51 326 354 625 1.67e-61 208
Msa0430860 AT2G28830.1 40.942 276 159 2 51 325 285 557 7.54e-59 199
Msa0430860 AT5G42340.1 39.310 290 172 1 39 328 369 654 3.93e-52 182
Msa0430860 AT3G07360.5 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430860 AT3G07360.3 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430860 AT3G07360.9 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430860 AT3G07360.6 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430860 AT3G07360.2 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430860 AT3G07360.8 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430860 AT3G07360.10 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430860 AT3G07360.7 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430860 AT3G07360.4 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430860 AT3G07360.1 33.681 288 181 8 49 330 169 452 3.61e-34 130
Msa0430860 AT3G03440.1 31.307 329 215 6 17 339 32 355 1.97e-33 128
Msa0430860 AT4G12710.1 33.557 298 184 8 50 337 46 339 1.49e-30 120
Msa0430860 AT1G29340.1 34.568 324 196 9 24 340 381 695 3.55e-27 112
Msa0430860 AT1G67530.2 31.321 265 176 5 72 332 447 709 3.10e-23 101
Msa0430860 AT1G67530.1 31.321 265 176 5 72 332 447 709 3.10e-23 101
Msa0430860 AT1G67530.4 31.321 265 176 5 72 332 447 709 3.10e-23 101
Msa0430860 AT5G18320.1 26.351 296 175 7 60 330 171 448 2.74e-22 97.8
Msa0430860 AT5G62560.1 28.159 277 188 6 58 329 243 513 4.02e-22 97.8
Msa0430860 AT5G40140.1 27.402 281 192 5 54 329 233 506 2.67e-21 95.1
Msa0430860 AT1G24330.1 29.924 264 178 5 73 332 448 708 4.11e-21 95.1
Msa0430860 AT1G24330.2 29.924 264 178 5 73 332 448 708 4.11e-21 95.1
Msa0430860 AT5G01830.1 30.686 277 173 8 62 330 380 645 7.31e-19 88.2
Msa0430860 AT1G27910.1 28.625 269 187 5 72 337 445 711 7.32e-19 88.2
Msa0430860 AT4G31890.3 29.180 305 184 11 49 329 132 428 2.57e-18 86.3
Msa0430860 AT4G31890.2 29.180 305 184 11 49 329 132 428 2.57e-18 86.3
Msa0430860 AT4G31890.1 29.180 305 184 11 49 329 132 428 2.57e-18 86.3
Msa0430860 AT5G65200.1 25.352 284 195 5 58 330 233 510 3.58e-17 82.8
Msa0430860 AT2G25130.1 27.985 268 179 7 72 329 132 395 1.09e-16 81.3
Msa0430860 AT4G21350.1 33.645 214 132 5 76 287 121 326 3.46e-16 79.3
Msa0430860 AT5G14510.1 30.108 279 180 8 58 329 9 279 4.49e-16 78.6
Msa0430860 AT3G47820.1 29.515 227 150 5 111 329 263 487 5.59e-16 79.3
Msa0430860 AT1G10560.1 29.258 229 148 7 68 287 407 630 2.52e-13 71.2
Msa0430860 AT5G13060.1 24.088 274 170 4 54 293 272 541 4.38e-13 70.9
Msa0430860 AT3G20170.1 27.530 247 166 7 84 328 144 379 1.46e-12 68.9
Msa0430860 AT1G77460.2 29.070 258 152 9 87 331 467 706 2.39e-12 68.9
Msa0430860 AT1G77460.3 29.070 258 152 9 87 331 467 706 2.39e-12 68.9
Msa0430860 AT1G77460.4 29.070 258 152 9 87 331 467 706 2.39e-12 68.9
Msa0430860 AT1G77460.1 29.070 258 152 9 87 331 467 706 2.39e-12 68.9
Msa0430860 AT3G60350.1 31.875 160 98 3 89 245 599 750 1.71e-11 65.9
Msa0430860 AT3G60350.2 31.875 160 98 3 89 245 599 750 1.71e-11 65.9
Msa0430860 AT5G18330.1 29.947 187 117 4 47 221 142 326 2.87e-11 64.7

Find 101 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGCCTTTGGTTGAATTAA+TGG 0.174563 3_2:-82719733 Msa0430860:CDS
TGAACGTGGAAAAGAGATTT+TGG 0.221914 3_2:+82720497 None:intergenic
CCGGCGACGGTGATGATAAA+AGG 0.253012 3_2:+82720099 None:intergenic
AAAGAGATTATCACTTCTTC+TGG 0.285237 3_2:-82720008 Msa0430860:CDS
AATGAGAAGGCGTTGGATTC+TGG 0.288615 3_2:+82720298 None:intergenic
TAACAGCGATACATAGTTAA+TGG 0.292630 3_2:-82717296 Msa0430860:three_prime_UTR
GGTTGAATTAATGGCGGATT+TGG 0.298923 3_2:-82719724 Msa0430860:CDS
AAAGAGAGACACTCTGTTAC+AGG 0.308641 3_2:+82717126 None:intergenic
TTGGTGGAGATTGTGGAGGT+TGG 0.310557 3_2:-82719603 Msa0430860:CDS
AAGGCGTTGGATTCTGGTTT+CGG 0.334331 3_2:+82720304 None:intergenic
TGGAAGATGTGGAGCAATTC+CGG 0.336516 3_2:-82719874 Msa0430860:CDS
GAAGAGAATAAAGGAGCAAT+TGG 0.355932 3_2:-82719894 Msa0430860:CDS
TGAAGGTGCTATTCCTCCTT+TGG 0.356535 3_2:-82719496 Msa0430860:CDS
TTGTAAACTAACCTTGTCTT+TGG 0.375974 3_2:+82719443 None:intergenic
CAAGTATATATGTTGATTAG+AGG 0.381732 3_2:+82720448 None:intergenic
TGGTGAGCACAAAGGATATC+TGG 0.396238 3_2:+82717335 None:intergenic
CGGTTTAATAGCGCCGGCTT+TGG 0.400784 3_2:+82720130 None:intergenic
ATCATCACCGTCGCCGGGGA+TGG 0.404318 3_2:-82720093 Msa0430860:CDS
ATGTATCGCTGTTAGATTGT+TGG 0.419071 3_2:+82717305 None:intergenic
GTCTTTGGCGCGAATGGTAC+CGG 0.420372 3_2:+82719458 None:intergenic
TTCTGGTGCGATTAAACCTA+TGG 0.424118 3_2:-82719991 Msa0430860:CDS
AAAATTAGAGCGGTGAAAGC+TGG 0.424978 3_2:-82719762 Msa0430860:CDS
TGGTGGAGATTGTGGAGGTT+GGG 0.434537 3_2:-82719602 Msa0430860:CDS
GGTGGAGGAAGGAGGGATTC+CGG 0.437700 3_2:-82719628 Msa0430860:CDS
TCGAGAAGGTTCACCAATGC+CGG 0.444467 3_2:+82719855 None:intergenic
TCACAGACAGAAGAGAATAA+AGG 0.451466 3_2:-82719903 Msa0430860:CDS
TTCGGTTGTAGTGGTGAATT+CGG 0.452339 3_2:+82720322 None:intergenic
GGAAGCGAGGACGGCGTTGG+TGG 0.454845 3_2:-82719646 Msa0430860:CDS
TCCGGTGTTGGTGGAGATTG+TGG 0.459535 3_2:-82719610 Msa0430860:CDS
GAGCGTGTTGGTGACGGTTG+TGG 0.461714 3_2:-82719667 Msa0430860:CDS
TTGGTTTCTCTTTCTCAGTC+CGG 0.462112 3_2:-82719477 Msa0430860:CDS
ACAGTGGCATTGCTAGATCT+TGG 0.468286 3_2:+82717389 None:intergenic
AGCGAGGACGGCGTTGGTGG+AGG 0.468776 3_2:-82719643 Msa0430860:CDS
AACCTTGTCTTTGGCGCGAA+TGG 0.469160 3_2:+82719452 None:intergenic
GGAAGGAGGGATTCCGGTGT+TGG 0.479242 3_2:-82719622 Msa0430860:CDS
GAGTTCGTCGGAATAATTAT+CGG 0.485596 3_2:+82720265 None:intergenic
AGCTGGGATAATGAAGCCTT+TGG 0.485681 3_2:-82719745 Msa0430860:CDS
GCTGCATTCTCCTTCGCAGT+TGG 0.494828 3_2:+82719945 None:intergenic
AGACACTAATAGAGCTTCTA+CGG 0.497235 3_2:-82717415 Msa0430860:CDS
GGCGGATTTGGATTCGTACA+TGG 0.497248 3_2:-82719712 Msa0430860:CDS
GGTTTATGTTGATATTTATG+AGG 0.498706 3_2:+82717361 None:intergenic
GTCTTCAAAGCTTTCACCAT+AGG 0.500526 3_2:+82719975 None:intergenic
TATCGTAACGAATTACGTAA+TGG 0.507898 3_2:-82720411 Msa0430860:exon
CGGCGACGGTGATGATAAAA+GGG 0.510450 3_2:+82720100 None:intergenic
ACGGCGTTGGTGGAGGAAGG+AGG 0.512484 3_2:-82719636 Msa0430860:CDS
ATGTGGAGCAATTCCGGCAT+TGG 0.515544 3_2:-82719868 Msa0430860:CDS
AAATTAGAGCGGTGAAAGCT+GGG 0.520695 3_2:-82719761 Msa0430860:CDS
TTGGTGAACCTTCTCGAAAA+CGG 0.521459 3_2:-82719849 Msa0430860:CDS
AATGAAGATCGAGAATCAAC+CGG 0.523414 3_2:+82720237 None:intergenic
CGAGTAATGGTCTGCCGTGA+AGG 0.523826 3_2:-82719513 Msa0430860:CDS
GCAGTCGCTGAAAGCGGAAG+AGG 0.524507 3_2:+82720361 None:intergenic
TGTGGAAGCGAGGACGGCGT+TGG 0.525083 3_2:-82719649 Msa0430860:CDS
GACGGTTGTGGAAGCGAGGA+CGG 0.527350 3_2:-82719655 Msa0430860:CDS
CGTCTCAGAATCGCCAAAGC+CGG 0.529146 3_2:-82720143 Msa0430860:CDS
CGGCGTTGGTGGAGGAAGGA+GGG 0.530478 3_2:-82719635 Msa0430860:CDS
AAGGAGGAATAGCACCTTCA+CGG 0.530668 3_2:+82719499 None:intergenic
AAAGGAGCAATTGGAAGATG+TGG 0.538963 3_2:-82719885 Msa0430860:CDS
AGGACGGCGTTGGTGGAGGA+AGG 0.544626 3_2:-82719639 Msa0430860:CDS
AGGAGGGATTCCGGTGTTGG+TGG 0.550459 3_2:-82719619 Msa0430860:CDS
ATGGTGAAAGCTTTGAAGAC+AGG 0.551125 3_2:-82719972 Msa0430860:CDS
TGTGATGAGCGTGTTGGTGA+CGG 0.551202 3_2:-82719673 Msa0430860:CDS
TGCAGTTGAAGATCCATCCC+CGG 0.553429 3_2:+82720080 None:intergenic
TAGTATAAATTATGCTAGCT+TGG 0.553785 3_2:-82717197 Msa0430860:three_prime_UTR
TCCGCCATTAATTCAACCAA+AGG 0.559491 3_2:+82719729 None:intergenic
TGAAGATCCATCCCCGGCGA+CGG 0.564290 3_2:+82720086 None:intergenic
TCGAAAACGGAGGAATCCGC+GGG 0.567283 3_2:-82719836 Msa0430860:CDS
ACAAAGCTTCTTCGTTACAA+AGG 0.569110 3_2:+82717230 None:intergenic
TCCACAATCTCCACCAACAC+CGG 0.569993 3_2:+82719609 None:intergenic
TACCATTCGCGCCAAAGACA+AGG 0.571154 3_2:-82719454 Msa0430860:intron
AATCAACGGTTTAATAGCGC+CGG 0.573314 3_2:+82720124 None:intergenic
CTCGAAAACGGAGGAATCCG+CGG 0.580066 3_2:-82719837 Msa0430860:CDS
GATTCTGGTTTCGGTTGTAG+TGG 0.584774 3_2:+82720313 None:intergenic
GAGCACAAAGGATATCTGGT+AGG 0.585677 3_2:+82717339 None:intergenic
GGTTGTAGTGGTGAATTCGG+TGG 0.586585 3_2:+82720325 None:intergenic
GCCTTTGGTTGAATTAATGG+CGG 0.592649 3_2:-82719730 Msa0430860:CDS
GAATATGAGTTATCGAGTAA+TGG 0.593225 3_2:-82719526 Msa0430860:CDS
CGGAAGCGCATGTAATGAGA+AGG 0.594684 3_2:+82720285 None:intergenic
GTTAGATTGTTGGAGTACAT+TGG 0.596753 3_2:+82717315 None:intergenic
ACTGTCGCAGTCGCTGAAAG+CGG 0.605550 3_2:+82720355 None:intergenic
CGCATGTAATGAGAAGGCGT+TGG 0.608908 3_2:+82720291 None:intergenic
GGTTGGGACGCAGAGACAGA+AGG 0.611749 3_2:-82719586 Msa0430860:CDS
AGCACAAAGGATATCTGGTA+GGG 0.612741 3_2:+82717340 None:intergenic
GTAACGAATTACGTAATGGA+AGG 0.615430 3_2:-82720407 Msa0430860:exon
ATTCCGACGAACTCATTCGC+CGG 0.620409 3_2:-82720256 Msa0430860:CDS
GACAGGCACTCCAACTGCGA+AGG 0.621740 3_2:-82719955 Msa0430860:CDS
GTTGATTAGAGGAAAAGTGA+TGG 0.624006 3_2:+82720459 None:intergenic
GACTGAGAAAGAGAAACCAA+AGG 0.629876 3_2:+82719480 None:intergenic
TATTTATGAGGCACGTACAG+TGG 0.637169 3_2:+82717373 None:intergenic
GATGATAAAAGGGAAATCAA+CGG 0.645026 3_2:+82720110 None:intergenic
GCAGAGACAGAAGGAAATTG+CGG 0.654422 3_2:-82719577 Msa0430860:CDS
CAACCGGCGAATGAGTTCGT+CGG 0.657343 3_2:+82720253 None:intergenic
GAGTACATTGGTGAGCACAA+AGG 0.660268 3_2:+82717327 None:intergenic
TGGTGACGGTTGTGGAAGCG+AGG 0.674684 3_2:-82719659 Msa0430860:CDS
GAGACAGAAGGAAATTGCGG+CGG 0.675465 3_2:-82719574 Msa0430860:CDS
GGTGTTGGTGGAGATTGTGG+AGG 0.676028 3_2:-82719607 Msa0430860:CDS
GAAAGAGAACAAAATTAGAG+CGG 0.684942 3_2:-82719772 Msa0430860:CDS
GGATTTGGATTCGTACATGG+TGG 0.694539 3_2:-82719709 Msa0430860:CDS
AGCGTATGTGATGAGCGTGT+TGG 0.695671 3_2:-82719679 Msa0430860:CDS
TGAGAAAGAGAAACCAAAGG+AGG 0.707414 3_2:+82719483 None:intergenic
TCTATATTATAAAGTGAACG+TGG 0.709631 3_2:+82720483 None:intergenic
GTGAACCTTCTCGAAAACGG+AGG 0.712449 3_2:-82719846 Msa0430860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATATTTTTTTTTGACA+TGG - chr3_2:82720073-82720092 Msa0430860:CDS 10.0%
!!! AATATATTTTTTTTTGACAT+GGG - chr3_2:82720074-82720093 Msa0430860:CDS 10.0%
!!! AAATTTTACTTTTACTGTAT+TGG - chr3_2:82718419-82718438 Msa0430860:intron 15.0%
!!! TTTCTTTTTTAAAGATTTGA+TGG - chr3_2:82718495-82718514 Msa0430860:intron 15.0%
!!! TTCTTTTTTAAAGATTTGAT+GGG - chr3_2:82718496-82718515 Msa0430860:intron 15.0%
!! AAAAGTAATTAAAACTCAAA+TGG - chr3_2:82718835-82718854 Msa0430860:intron 15.0%
!!! AACATTAACCTTTTAATATA+TGG - chr3_2:82719057-82719076 Msa0430860:intron 15.0%
!!! TCTAAATATTATTTTCCTAA+GGG - chr3_2:82719277-82719296 Msa0430860:intron 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG + chr3_2:82719374-82719393 None:intergenic 15.0%
!! AAATTATCTTAATCAACTAT+TGG + chr3_2:82719511-82719530 None:intergenic 15.0%
!! AATAGTTGATTAAGATAATT+TGG - chr3_2:82719510-82719529 Msa0430860:CDS 15.0%
!!! TTTACATCATTTTTTTTGAA+AGG + chr3_2:82719821-82719840 None:intergenic 15.0%
!!! TGATATTTTCTTTTCATCAA+TGG - chr3_2:82718459-82718478 Msa0430860:intron 20.0%
!!! TCTTTTTTAAAGATTTGATG+GGG - chr3_2:82718497-82718516 Msa0430860:intron 20.0%
!!! TTATAAAGTGTGTTTTATTG+AGG - chr3_2:82718696-82718715 Msa0430860:intron 20.0%
!! ATATGTAACGTATACATATA+TGG - chr3_2:82718859-82718878 Msa0430860:intron 20.0%
!! AAAGGTTAATGTTAAATTCA+AGG + chr3_2:82719050-82719069 None:intergenic 20.0%
!! ATTATGTAGAATTGATGTTT+GGG - chr3_2:82719252-82719271 Msa0430860:intron 20.0%
!!! GTCTAAATATTATTTTCCTA+AGG - chr3_2:82719276-82719295 Msa0430860:intron 20.0%
!! GTAACATAGTATTAATTGAA+GGG + chr3_2:82719448-82719467 None:intergenic 20.0%
!! TGTAACATAGTATTAATTGA+AGG + chr3_2:82719449-82719468 None:intergenic 20.0%
!! TGATTATGATAAACTTACAA+AGG + chr3_2:82719708-82719727 None:intergenic 20.0%
! TCTATATTATAAAGTGAACG+TGG + chr3_2:82717150-82717169 None:intergenic 25.0%
!! CAAGTATATATGTTGATTAG+AGG + chr3_2:82717185-82717204 None:intergenic 25.0%
!! TTTTTGTTGATCAATAGAAG+AGG + chr3_2:82717419-82717438 None:intergenic 25.0%
!!! ACGATTTTCTGATTTGTTTT+TGG + chr3_2:82717470-82717489 None:intergenic 25.0%
!!! ATTTTATACGGTGTTGAATA+AGG + chr3_2:82718777-82718796 None:intergenic 25.0%
! CATTATGTAGAATTGATGTT+TGG - chr3_2:82719251-82719270 Msa0430860:intron 25.0%
!! TATTATTTTCCTAAGGGTTA+AGG - chr3_2:82719283-82719302 Msa0430860:intron 25.0%
!!! AAACGACACTCATTTTAAAA+CGG + chr3_2:82719348-82719367 None:intergenic 25.0%
! GAAAATTGATCTACTCTAAA+TGG - chr3_2:82719660-82719679 Msa0430860:CDS 25.0%
! GTCAAAAAAATTATGCAAAG+TGG + chr3_2:82719853-82719872 None:intergenic 25.0%
!!! CTTTGCATAATTTTTTTGAC+AGG - chr3_2:82719853-82719872 Msa0430860:CDS 25.0%
!!! TATTTTGATTTCGTGTCTAA+TGG - chr3_2:82720119-82720138 Msa0430860:CDS 25.0%
! GGTTTATGTTGATATTTATG+AGG + chr3_2:82720272-82720291 None:intergenic 25.0%
!!! TATACAAGTTGATGCTTTTA+GGG + chr3_2:82720376-82720395 None:intergenic 25.0%
!!! ATATACAAGTTGATGCTTTT+AGG + chr3_2:82720377-82720396 None:intergenic 25.0%
! TAGTATAAATTATGCTAGCT+TGG - chr3_2:82720433-82720452 Msa0430860:five_prime_UTR 25.0%
TATCGTAACGAATTACGTAA+TGG - chr3_2:82717219-82717238 Msa0430860:three_prime_UTR 30.0%
TCAACAAAAACAAGCTACAA+TGG - chr3_2:82717429-82717448 Msa0430860:intron 30.0%
GATGATAAAAGGGAAATCAA+CGG + chr3_2:82717523-82717542 None:intergenic 30.0%
AAAGAGATTATCACTTCTTC+TGG - chr3_2:82717622-82717641 Msa0430860:intron 30.0%
GAAAGAGAACAAAATTAGAG+CGG - chr3_2:82717858-82717877 Msa0430860:intron 30.0%
GAATATGAGTTATCGAGTAA+TGG - chr3_2:82718104-82718123 Msa0430860:intron 30.0%
TTGTAAACTAACCTTGTCTT+TGG + chr3_2:82718190-82718209 None:intergenic 30.0%
! ACCTTTTAATATATGGTGTC+CGG - chr3_2:82719064-82719083 Msa0430860:intron 30.0%
!! TTTAAAACGGAGAGAGTATT+TGG + chr3_2:82719335-82719354 None:intergenic 30.0%
!! TTCCTTAATTTGTGTGCAAT+TGG - chr3_2:82719567-82719586 Msa0430860:CDS 30.0%
AACCAATTGCACACAAATTA+AGG + chr3_2:82719572-82719591 None:intergenic 30.0%
TAACAGCGATACATAGTTAA+TGG - chr3_2:82720334-82720353 Msa0430860:CDS 30.0%
! TGAACGTGGAAAAGAGATTT+TGG + chr3_2:82717136-82717155 None:intergenic 35.0%
GTTGATTAGAGGAAAAGTGA+TGG + chr3_2:82717174-82717193 None:intergenic 35.0%
! GTAACGAATTACGTAATGGA+AGG - chr3_2:82717223-82717242 Msa0430860:three_prime_UTR 35.0%
GAGTTCGTCGGAATAATTAT+CGG + chr3_2:82717368-82717387 None:intergenic 35.0%
AATGAAGATCGAGAATCAAC+CGG + chr3_2:82717396-82717415 None:intergenic 35.0%
! TATTTTCATCGCATAGTGAG+AGG + chr3_2:82717603-82717622 None:intergenic 35.0%
TCACAGACAGAAGAGAATAA+AGG - chr3_2:82717727-82717746 Msa0430860:intron 35.0%
GAAGAGAATAAAGGAGCAAT+TGG - chr3_2:82717736-82717755 Msa0430860:intron 35.0%
GAAGCCTTTGGTTGAATTAA+TGG - chr3_2:82717897-82717916 Msa0430860:intron 35.0%
TACAACTCTTCATGTTTACG+CGG - chr3_2:82718205-82718224 Msa0430860:intron 35.0%
TGCAATTTGCAATTGCAAGA+TGG + chr3_2:82718258-82718277 None:intergenic 35.0%
TCCGGACACCATATATTAAA+AGG + chr3_2:82719068-82719087 None:intergenic 35.0%
TAATATATGGTGTCCGGATT+CGG - chr3_2:82719070-82719089 Msa0430860:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr3_2:82719593-82719612 Msa0430860:CDS 35.0%
! TTGTTGATTGTTCCTCTTCA+TGG - chr3_2:82720180-82720199 Msa0430860:CDS 35.0%
! TGTTGATTGTTCCTCTTCAT+GGG - chr3_2:82720181-82720200 Msa0430860:CDS 35.0%
AGACACTAATAGAGCTTCTA+CGG - chr3_2:82720215-82720234 Msa0430860:CDS 35.0%
GTTAGATTGTTGGAGTACAT+TGG + chr3_2:82720318-82720337 None:intergenic 35.0%
ATGTATCGCTGTTAGATTGT+TGG + chr3_2:82720328-82720347 None:intergenic 35.0%
ACAAAGCTTCTTCGTTACAA+AGG + chr3_2:82720403-82720422 None:intergenic 35.0%
!! CTTAGTATGCTTTGCTTTTC+TGG - chr3_2:82720464-82720483 Msa0430860:five_prime_UTR 35.0%
TTCGGTTGTAGTGGTGAATT+CGG + chr3_2:82717311-82717330 None:intergenic 40.0%
AATCAACGGTTTAATAGCGC+CGG + chr3_2:82717509-82717528 None:intergenic 40.0%
TTCTGGTGCGATTAAACCTA+TGG - chr3_2:82717639-82717658 Msa0430860:intron 40.0%
GTCTTCAAAGCTTTCACCAT+AGG + chr3_2:82717658-82717677 None:intergenic 40.0%
!! ATGGTGAAAGCTTTGAAGAC+AGG - chr3_2:82717658-82717677 Msa0430860:intron 40.0%
AAAGGAGCAATTGGAAGATG+TGG - chr3_2:82717745-82717764 Msa0430860:intron 40.0%
TTGGTGAACCTTCTCGAAAA+CGG - chr3_2:82717781-82717800 Msa0430860:intron 40.0%
AAAATTAGAGCGGTGAAAGC+TGG - chr3_2:82717868-82717887 Msa0430860:intron 40.0%
AAATTAGAGCGGTGAAAGCT+GGG - chr3_2:82717869-82717888 Msa0430860:intron 40.0%
GCCTTTGGTTGAATTAATGG+CGG - chr3_2:82717900-82717919 Msa0430860:intron 40.0%
TCCGCCATTAATTCAACCAA+AGG + chr3_2:82717904-82717923 None:intergenic 40.0%
!! GGTTGAATTAATGGCGGATT+TGG - chr3_2:82717906-82717925 Msa0430860:intron 40.0%
TGAGAAAGAGAAACCAAAGG+AGG + chr3_2:82718150-82718169 None:intergenic 40.0%
GACTGAGAAAGAGAAACCAA+AGG + chr3_2:82718153-82718172 None:intergenic 40.0%
TTGGTTTCTCTTTCTCAGTC+CGG - chr3_2:82718153-82718172 Msa0430860:intron 40.0%
TGGAAGAATTACTGCTGACA+TGG + chr3_2:82718238-82718257 None:intergenic 40.0%
TTGGTAAGGACAATGCATCA+AGG + chr3_2:82719104-82719123 None:intergenic 40.0%
ATTTGGATGAGCACAATGCA+TGG + chr3_2:82719318-82719337 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr3_2:82719597-82719616 Msa0430860:CDS 40.0%
TGATTTCGTGTCTAATGGTG+TGG - chr3_2:82720124-82720143 Msa0430860:CDS 40.0%
GTTGATTGTTCCTCTTCATG+GGG - chr3_2:82720182-82720201 Msa0430860:CDS 40.0%
TATTTATGAGGCACGTACAG+TGG + chr3_2:82720260-82720279 None:intergenic 40.0%
AGCACAAAGGATATCTGGTA+GGG + chr3_2:82720293-82720312 None:intergenic 40.0%
!!! CAAGTTGATGCTTTTAGGGT+GGG + chr3_2:82720372-82720391 None:intergenic 40.0%
!!! ACAAGTTGATGCTTTTAGGG+TGG + chr3_2:82720373-82720392 None:intergenic 40.0%
!! GATTCTGGTTTCGGTTGTAG+TGG + chr3_2:82717320-82717339 None:intergenic 45.0%
!! AAGGCGTTGGATTCTGGTTT+CGG + chr3_2:82717329-82717348 None:intergenic 45.0%
!! AATGAGAAGGCGTTGGATTC+TGG + chr3_2:82717335-82717354 None:intergenic 45.0%
TGGAAGATGTGGAGCAATTC+CGG - chr3_2:82717756-82717775 Msa0430860:intron 45.0%
!!! TTGACGCATCTTTTTTCCCG+CGG + chr3_2:82717813-82717832 None:intergenic 45.0%
CGGGAAAAAAGATGCGTCAA+CGG - chr3_2:82717813-82717832 Msa0430860:intron 45.0%
AGCTGGGATAATGAAGCCTT+TGG - chr3_2:82717885-82717904 Msa0430860:intron 45.0%
GGATTTGGATTCGTACATGG+TGG - chr3_2:82717921-82717940 Msa0430860:intron 45.0%
GCAGAGACAGAAGGAAATTG+CGG - chr3_2:82718053-82718072 Msa0430860:intron 45.0%
AAGGAGGAATAGCACCTTCA+CGG + chr3_2:82718134-82718153 None:intergenic 45.0%
!! TGAAGGTGCTATTCCTCCTT+TGG - chr3_2:82718134-82718153 Msa0430860:intron 45.0%
TTACCAACTGAGCTAGCTCA+CGG - chr3_2:82719117-82719136 Msa0430860:intron 45.0%
AAGGACATGCCTTAACCCTT+AGG + chr3_2:82719295-82719314 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr3_2:82719596-82719615 Msa0430860:CDS 45.0%
ACAGTGGCATTGCTAGATCT+TGG + chr3_2:82720244-82720263 None:intergenic 45.0%
GAGCACAAAGGATATCTGGT+AGG + chr3_2:82720294-82720313 None:intergenic 45.0%
TGGTGAGCACAAAGGATATC+TGG + chr3_2:82720298-82720317 None:intergenic 45.0%
GAGTACATTGGTGAGCACAA+AGG + chr3_2:82720306-82720325 None:intergenic 45.0%
!!! ATTATAAATAACATTTTATA+CGG + chr3_2:82718789-82718808 None:intergenic 5.0%
!! ATTAAAGAAAAAATAATATT+TGG + chr3_2:82719478-82719497 None:intergenic 5.0%
!! AAATAAATAAATCTAATAAA+AGG + chr3_2:82719760-82719779 None:intergenic 5.0%
GGTTGTAGTGGTGAATTCGG+TGG + chr3_2:82717308-82717327 None:intergenic 50.0%
! CGCATGTAATGAGAAGGCGT+TGG + chr3_2:82717342-82717361 None:intergenic 50.0%
! CGGAAGCGCATGTAATGAGA+AGG + chr3_2:82717348-82717367 None:intergenic 50.0%
ATTCCGACGAACTCATTCGC+CGG - chr3_2:82717374-82717393 Msa0430860:exon 50.0%
CGGCGACGGTGATGATAAAA+GGG + chr3_2:82717533-82717552 None:intergenic 50.0%
! CCTTTTATCATCACCGTCGC+CGG - chr3_2:82717531-82717550 Msa0430860:intron 50.0%
! CTTTTATCATCACCGTCGCC+GGG - chr3_2:82717532-82717551 Msa0430860:intron 50.0%
! TTTTATCATCACCGTCGCCG+GGG - chr3_2:82717533-82717552 Msa0430860:intron 50.0%
TGCAGTTGAAGATCCATCCC+CGG + chr3_2:82717553-82717572 None:intergenic 50.0%
ATGTGGAGCAATTCCGGCAT+TGG - chr3_2:82717762-82717781 Msa0430860:intron 50.0%
TCGAGAAGGTTCACCAATGC+CGG + chr3_2:82717778-82717797 None:intergenic 50.0%
GTGAACCTTCTCGAAAACGG+AGG - chr3_2:82717784-82717803 Msa0430860:intron 50.0%
!! GGATTCCTCCGTTTTCGAGA+AGG + chr3_2:82717792-82717811 None:intergenic 50.0%
! GGCGGATTTGGATTCGTACA+TGG - chr3_2:82717918-82717937 Msa0430860:intron 50.0%
AGCGTATGTGATGAGCGTGT+TGG - chr3_2:82717951-82717970 Msa0430860:intron 50.0%
! TGTGATGAGCGTGTTGGTGA+CGG - chr3_2:82717957-82717976 Msa0430860:intron 50.0%
TCCACAATCTCCACCAACAC+CGG + chr3_2:82718024-82718043 None:intergenic 50.0%
TTGGTGGAGATTGTGGAGGT+TGG - chr3_2:82718027-82718046 Msa0430860:intron 50.0%
TGGTGGAGATTGTGGAGGTT+GGG - chr3_2:82718028-82718047 Msa0430860:intron 50.0%
GAGACAGAAGGAAATTGCGG+CGG - chr3_2:82718056-82718075 Msa0430860:intron 50.0%
TACCATTCGCGCCAAAGACA+AGG - chr3_2:82718176-82718195 Msa0430860:intron 50.0%
AACCTTGTCTTTGGCGCGAA+TGG + chr3_2:82718181-82718200 None:intergenic 50.0%
GGACAATGCATCAAGGTCTG+AGG + chr3_2:82719097-82719116 None:intergenic 50.0%
! GTGAGCTAGCTCAGTTGGTA+AGG + chr3_2:82719118-82719137 None:intergenic 50.0%
GGATGAGCACAATGCATGGA+AGG + chr3_2:82719314-82719333 None:intergenic 50.0%
ACTGTCGCAGTCGCTGAAAG+CGG + chr3_2:82717278-82717297 None:intergenic 55.0%
CAACCGGCGAATGAGTTCGT+CGG + chr3_2:82717380-82717399 None:intergenic 55.0%
CGTCTCAGAATCGCCAAAGC+CGG - chr3_2:82717487-82717506 Msa0430860:intron 55.0%
!! CGGTTTAATAGCGCCGGCTT+TGG + chr3_2:82717503-82717522 None:intergenic 55.0%
CCGGCGACGGTGATGATAAA+AGG + chr3_2:82717534-82717553 None:intergenic 55.0%
GCTGCATTCTCCTTCGCAGT+TGG + chr3_2:82717688-82717707 None:intergenic 55.0%
CTCGAAAACGGAGGAATCCG+CGG - chr3_2:82717793-82717812 Msa0430860:intron 55.0%
TCGAAAACGGAGGAATCCGC+GGG - chr3_2:82717794-82717813 Msa0430860:intron 55.0%
!! TCCGGTGTTGGTGGAGATTG+TGG - chr3_2:82718020-82718039 Msa0430860:intron 55.0%
!! GGTGTTGGTGGAGATTGTGG+AGG - chr3_2:82718023-82718042 Msa0430860:intron 55.0%
CGAGTAATGGTCTGCCGTGA+AGG - chr3_2:82718117-82718136 Msa0430860:intron 55.0%
GTCTTTGGCGCGAATGGTAC+CGG + chr3_2:82718175-82718194 None:intergenic 55.0%
! CAAGGTCTGAGGTCCGAATC+CGG + chr3_2:82719086-82719105 None:intergenic 55.0%
TCTCCGTGAGCTAGCTCAGT+TGG + chr3_2:82719123-82719142 None:intergenic 55.0%
GTTCCTCTTCATGGGGACAC+AGG - chr3_2:82720189-82720208 Msa0430860:CDS 55.0%
CTACCTGTGTCCCCATGAAG+AGG + chr3_2:82720195-82720214 None:intergenic 55.0%
GCAGTCGCTGAAAGCGGAAG+AGG + chr3_2:82717272-82717291 None:intergenic 60.0%
TGAAGATCCATCCCCGGCGA+CGG + chr3_2:82717547-82717566 None:intergenic 60.0%
GACAGGCACTCCAACTGCGA+AGG - chr3_2:82717675-82717694 Msa0430860:intron 60.0%
!! GAGCGTGTTGGTGACGGTTG+TGG - chr3_2:82717963-82717982 Msa0430860:intron 60.0%
!! TGGTGACGGTTGTGGAAGCG+AGG - chr3_2:82717971-82717990 Msa0430860:intron 60.0%
GACGGTTGTGGAAGCGAGGA+CGG - chr3_2:82717975-82717994 Msa0430860:intron 60.0%
GGTGGAGGAAGGAGGGATTC+CGG - chr3_2:82718002-82718021 Msa0430860:intron 60.0%
GGAAGGAGGGATTCCGGTGT+TGG - chr3_2:82718008-82718027 Msa0430860:intron 60.0%
! AGGAGGGATTCCGGTGTTGG+TGG - chr3_2:82718011-82718030 Msa0430860:intron 60.0%
GGTTGGGACGCAGAGACAGA+AGG - chr3_2:82718044-82718063 Msa0430860:intron 60.0%
ATCATCACCGTCGCCGGGGA+TGG - chr3_2:82717537-82717556 Msa0430860:intron 65.0%
TGTGGAAGCGAGGACGGCGT+TGG - chr3_2:82717981-82718000 Msa0430860:intron 65.0%
!! AGGACGGCGTTGGTGGAGGA+AGG - chr3_2:82717991-82718010 Msa0430860:intron 65.0%
!! ACGGCGTTGGTGGAGGAAGG+AGG - chr3_2:82717994-82718013 Msa0430860:intron 65.0%
!! CGGCGTTGGTGGAGGAAGGA+GGG - chr3_2:82717995-82718014 Msa0430860:intron 65.0%
! GGAAGCGAGGACGGCGTTGG+TGG - chr3_2:82717984-82718003 Msa0430860:intron 70.0%
!! AGCGAGGACGGCGTTGGTGG+AGG - chr3_2:82717987-82718006 Msa0430860:intron 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 82717133 82720519 82717133 ID=Msa0430860;Name=Msa0430860
chr3_2 mRNA 82717133 82720519 82717133 ID=Msa0430860-mRNA-1;Parent=Msa0430860;Name=Msa0430860-mRNA-1;_AED=0.02;_eAED=0.02;_QI=105|1|1|1|1|1|2|243|341
chr3_2 exon 82717133 82717441 82717133 ID=Msa0430860-mRNA-1:exon:23017;Parent=Msa0430860-mRNA-1
chr3_2 exon 82719455 82720519 82719455 ID=Msa0430860-mRNA-1:exon:23016;Parent=Msa0430860-mRNA-1
chr3_2 five_prime_UTR 82720415 82720519 82720415 ID=Msa0430860-mRNA-1:five_prime_utr;Parent=Msa0430860-mRNA-1
chr3_2 CDS 82719455 82720414 82719455 ID=Msa0430860-mRNA-1:cds;Parent=Msa0430860-mRNA-1
chr3_2 CDS 82717376 82717441 82717376 ID=Msa0430860-mRNA-1:cds;Parent=Msa0430860-mRNA-1
chr3_2 three_prime_UTR 82717133 82717375 82717133 ID=Msa0430860-mRNA-1:three_prime_utr;Parent=Msa0430860-mRNA-1
Gene Sequence

>Msa0430860

ATGGAAGGAACTTTCAATTTCAACGATAATTCCTCTTCCGCTTTCAGCGACTGCGACAGTGACAGATCCACCGAATTCACCACTACAACCGAAACCAGAATCCAACGCCTTCTCATTACATGCGCTTCCGATAATTATTCCGACGAACTCATTCGCCGGTTGATTCTCGATCTTCATTCCTCTTCTATTGATCAACAAAAACAAGCTACAATGGAAATCAGATTACTCGCCAAAAACAAATCAGAAAATCGTCTCAGAATCGCCAAAGCCGGCGCTATTAAACCGTTGATTTCCCTTTTATCATCACCGTCGCCGGGGATGGATCTTCAACTGCAAGAATACGTCGTCACAGCGATTTTAAACCTCTCACTATGCGATGAAAATAAAGAGATTATCACTTCTTCTGGTGCGATTAAACCTATGGTGAAAGCTTTGAAGACAGGCACTCCAACTGCGAAGGAGAATGCAGCATGTGCTCTGCTTCGTCTCTCACAGACAGAAGAGAATAAAGGAGCAATTGGAAGATGTGGAGCAATTCCGGCATTGGTGAACCTTCTCGAAAACGGAGGAATCCGCGGGAAAAAAGATGCGTCAACGGCGTTATACACGTTGTGTTCTGTGAAAGAGAACAAAATTAGAGCGGTGAAAGCTGGGATAATGAAGCCTTTGGTTGAATTAATGGCGGATTTGGATTCGTACATGGTGGATAAGTCAGCGTATGTGATGAGCGTGTTGGTGACGGTTGTGGAAGCGAGGACGGCGTTGGTGGAGGAAGGAGGGATTCCGGTGTTGGTGGAGATTGTGGAGGTTGGGACGCAGAGACAGAAGGAAATTGCGGCGGCGATTTTGTTGAAGATTTGTGAAGAGAATATGAGTTATCGAGTAATGGTCTGCCGTGAAGGTGCTATTCCTCCTTTGGTTTCTCTTTCTCAGTCCGGTACCATTCGCGCCAAAGACAAGGTAGAGACACTAATAGAGCTTCTACGGCAACCAAGATCTAGCAATGCCACTGTACGTGCCTCATAA
Protein sequence

>Msa0430860

MEGTFNFNDNSSSAFSDCDSDRSTEFTTTTETRIQRLLITCASDNYSDELIRRLILDLHSSSIDQQKQATMEIRLLAKNKSENRLRIAKAGAIKPLISLLSSPSPGMDLQLQEYVVTAILNLSLCDENKEIITSSGAIKPMVKALKTGTPTAKENAACALLRLSQTEENKGAIGRCGAIPALVNLLENGGIRGKKDASTALYTLCSVKENKIRAVKAGIMKPLVELMADLDSYMVDKSAYVMSVLVTVVEARTALVEEGGIPVLVEIVEVGTQRQKEIAAAILLKICEENMSYRVMVCREGAIPPLVSLSQSGTIRAKDKVETLIELLRQPRSSNATVRAS*