AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0430960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0430960 MtrunA17_Chr3g0134761 95.601 341 15 0 1 341 1 341 0.0 657
Msa0430960 MtrunA17_Chr1g0152561 76.737 331 70 4 5 334 16 340 3.71e-178 497
Msa0430960 MtrunA17_Chr2g0290151 49.020 306 140 3 44 337 156 457 2.62e-83 259
Msa0430960 MtrunA17_Chr1g0183781 49.477 287 140 2 51 337 440 721 5.19e-80 258
Msa0430960 MtrunA17_Chr3g0106931 51.748 286 133 2 51 336 553 833 1.96e-77 253
Msa0430960 MtrunA17_Chr7g0261371 46.809 282 145 2 51 332 514 790 1.90e-70 234
Msa0430960 MtrunA17_Chr4g0044411 45.357 280 149 1 51 330 373 648 1.58e-67 223
Msa0430960 MtrunA17_Chr4g0031431 43.262 282 156 1 51 332 353 630 2.00e-61 207
Msa0430960 MtrunA17_Chr7g0248251 40.794 277 160 1 54 330 370 642 2.52e-61 207
Msa0430960 MtrunA17_Chr1g0199321 45.714 280 148 1 51 330 345 620 1.65e-60 204
Msa0430960 MtrunA17_Chr7g0237931 41.581 291 155 4 43 328 325 605 1.28e-58 199
Msa0430960 MtrunA17_Chr5g0437411 39.655 290 162 6 54 334 408 693 7.00e-47 168
Msa0430960 MtrunA17_Chr5g0429231 35.379 277 170 5 58 329 41 313 6.12e-38 138
Msa0430960 MtrunA17_Chr7g0275081 36.170 282 168 7 53 326 171 448 8.79e-35 132
Msa0430960 MtrunA17_Chr2g0284461 37.329 292 169 9 54 339 399 682 1.31e-33 131
Msa0430960 MtrunA17_Chr7g0234671 32.616 279 183 4 55 330 175 451 2.24e-31 122
Msa0430960 MtrunA17_Chr5g0435041 33.803 284 177 6 51 329 20 297 4.96e-29 115
Msa0430960 MtrunA17_Chr1g0189811 34.375 288 183 5 51 334 157 442 8.84e-27 110
Msa0430960 MtrunA17_Chr3g0118001 26.882 279 195 3 54 329 214 486 8.75e-26 107
Msa0430960 MtrunA17_Chr6g0449461 30.479 292 184 8 52 333 3 285 2.92e-25 103
Msa0430960 MtrunA17_Chr3g0111451 34.708 291 176 7 51 333 41 325 1.77e-24 102
Msa0430960 MtrunA17_Chr8g0377151 31.985 272 178 6 72 338 449 718 6.37e-24 102
Msa0430960 MtrunA17_Chr4g0013091 30.690 290 185 8 50 330 416 698 1.11e-20 93.2
Msa0430960 MtrunA17_Chr4g0059201 29.412 306 198 10 42 339 417 712 7.16e-20 90.9
Msa0430960 MtrunA17_Chr6g0451581 25.822 213 153 2 116 325 299 509 1.17e-17 84.0
Msa0430960 MtrunA17_Chr1g0149941 27.931 290 190 9 53 329 175 458 1.74e-17 83.6
Msa0430960 MtrunA17_Chr3g0122721 28.947 266 181 6 72 332 448 710 2.33e-17 83.2
Msa0430960 MtrunA17_Chr3g0137061 27.491 291 193 7 51 329 110 394 4.72e-17 82.0
Msa0430960 MtrunA17_Chr3g0117851 27.586 290 160 8 47 330 378 623 7.25e-16 78.6
Msa0430960 MtrunA17_Chr4g0048811 30.882 204 134 6 129 329 121 320 1.85e-15 76.3
Msa0430960 MtrunA17_Chr1g0199031 39.496 119 55 1 132 250 18 119 2.40e-15 72.0
Msa0430960 MtrunA17_Chr8g0350681 28.251 223 146 7 91 309 238 450 8.49e-15 75.5
Msa0430960 MtrunA17_Chr2g0280671 29.008 262 166 9 75 329 131 379 2.67e-13 70.1
Msa0430960 MtrunA17_Chr7g0259851 25.214 234 163 3 58 291 276 497 3.56e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0430960 AT3G01400.1 73.824 340 78 7 5 341 21 352 1.13e-170 479
Msa0430960 AT5G58680.1 69.277 332 91 6 4 332 18 341 1.25e-158 448
Msa0430960 AT4G16490.1 50.505 297 141 2 38 332 172 464 1.10e-83 261
Msa0430960 AT2G23140.3 45.181 332 169 4 13 336 308 634 7.91e-81 258
Msa0430960 AT2G23140.5 45.181 332 169 4 13 336 308 634 7.91e-81 258
Msa0430960 AT2G23140.2 45.181 332 169 4 13 336 497 823 3.63e-79 258
Msa0430960 AT2G23140.4 45.181 332 169 4 13 336 497 823 3.63e-79 258
Msa0430960 AT2G23140.1 45.181 332 169 4 13 336 500 826 4.17e-79 258
Msa0430960 AT5G67340.1 48.252 286 142 3 51 335 423 703 9.41e-74 241
Msa0430960 AT5G67340.2 48.252 286 142 3 51 335 423 703 9.41e-74 241
Msa0430960 AT3G54790.2 48.057 283 141 3 53 334 440 717 3.77e-70 232
Msa0430960 AT3G54790.3 48.057 283 141 3 53 334 476 753 1.35e-69 231
Msa0430960 AT3G54790.1 48.057 283 141 3 53 334 476 753 1.35e-69 231
Msa0430960 AT1G23030.1 45.035 282 150 2 50 330 332 609 1.08e-68 226
Msa0430960 AT1G71020.2 45.907 281 148 2 51 330 195 472 1.21e-68 223
Msa0430960 AT1G71020.1 45.907 281 148 2 51 330 343 620 1.54e-67 223
Msa0430960 AT3G54850.1 47.518 282 142 3 50 330 346 622 1.11e-62 211
Msa0430960 AT3G46510.1 42.391 276 155 1 51 326 354 625 1.67e-61 208
Msa0430960 AT2G28830.1 40.942 276 159 2 51 325 285 557 7.54e-59 199
Msa0430960 AT5G42340.1 39.310 290 172 1 39 328 369 654 3.93e-52 182
Msa0430960 AT3G07360.5 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430960 AT3G07360.3 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430960 AT3G07360.9 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430960 AT3G07360.6 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430960 AT3G07360.2 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430960 AT3G07360.8 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430960 AT3G07360.10 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430960 AT3G07360.7 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430960 AT3G07360.4 33.681 288 181 8 49 330 34 317 2.66e-35 131
Msa0430960 AT3G07360.1 33.681 288 181 8 49 330 169 452 3.61e-34 130
Msa0430960 AT3G03440.1 31.307 329 215 6 17 339 32 355 1.97e-33 128
Msa0430960 AT4G12710.1 33.557 298 184 8 50 337 46 339 1.49e-30 120
Msa0430960 AT1G29340.1 34.568 324 196 9 24 340 381 695 3.55e-27 112
Msa0430960 AT1G67530.2 31.321 265 176 5 72 332 447 709 3.10e-23 101
Msa0430960 AT1G67530.1 31.321 265 176 5 72 332 447 709 3.10e-23 101
Msa0430960 AT1G67530.4 31.321 265 176 5 72 332 447 709 3.10e-23 101
Msa0430960 AT5G18320.1 26.351 296 175 7 60 330 171 448 2.74e-22 97.8
Msa0430960 AT5G62560.1 28.159 277 188 6 58 329 243 513 4.02e-22 97.8
Msa0430960 AT5G40140.1 27.402 281 192 5 54 329 233 506 2.67e-21 95.1
Msa0430960 AT1G24330.1 29.924 264 178 5 73 332 448 708 4.11e-21 95.1
Msa0430960 AT1G24330.2 29.924 264 178 5 73 332 448 708 4.11e-21 95.1
Msa0430960 AT5G01830.1 30.686 277 173 8 62 330 380 645 7.31e-19 88.2
Msa0430960 AT1G27910.1 28.625 269 187 5 72 337 445 711 7.32e-19 88.2
Msa0430960 AT4G31890.3 29.180 305 184 11 49 329 132 428 2.57e-18 86.3
Msa0430960 AT4G31890.2 29.180 305 184 11 49 329 132 428 2.57e-18 86.3
Msa0430960 AT4G31890.1 29.180 305 184 11 49 329 132 428 2.57e-18 86.3
Msa0430960 AT5G65200.1 25.352 284 195 5 58 330 233 510 3.58e-17 82.8
Msa0430960 AT2G25130.1 27.985 268 179 7 72 329 132 395 1.09e-16 81.3
Msa0430960 AT4G21350.1 33.645 214 132 5 76 287 121 326 3.46e-16 79.3
Msa0430960 AT5G14510.1 30.108 279 180 8 58 329 9 279 4.49e-16 78.6
Msa0430960 AT3G47820.1 29.515 227 150 5 111 329 263 487 5.59e-16 79.3
Msa0430960 AT1G10560.1 29.258 229 148 7 68 287 407 630 2.52e-13 71.2
Msa0430960 AT5G13060.1 24.088 274 170 4 54 293 272 541 4.38e-13 70.9
Msa0430960 AT3G20170.1 27.530 247 166 7 84 328 144 379 1.46e-12 68.9
Msa0430960 AT1G77460.2 29.070 258 152 9 87 331 467 706 2.39e-12 68.9
Msa0430960 AT1G77460.3 29.070 258 152 9 87 331 467 706 2.39e-12 68.9
Msa0430960 AT1G77460.4 29.070 258 152 9 87 331 467 706 2.39e-12 68.9
Msa0430960 AT1G77460.1 29.070 258 152 9 87 331 467 706 2.39e-12 68.9
Msa0430960 AT3G60350.1 31.875 160 98 3 89 245 599 750 1.71e-11 65.9
Msa0430960 AT3G60350.2 31.875 160 98 3 89 245 599 750 1.71e-11 65.9
Msa0430960 AT5G18330.1 29.947 187 117 4 47 221 142 326 2.87e-11 64.7

Find 101 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGCCTTTGGTTGAATTAA+TGG 0.174563 3_2:+82817585 Msa0430960:CDS
TGAACGTGGAAAAGAGATTT+TGG 0.221914 3_2:-82816821 None:intergenic
CCGGCGACGGTGATGATAAA+AGG 0.253012 3_2:-82817219 None:intergenic
AAAGAGATTATCACTTCTTC+TGG 0.285237 3_2:+82817310 Msa0430960:CDS
AATGAGAAGGCGTTGGATTC+TGG 0.288615 3_2:-82817020 None:intergenic
TAACAGCGATACATAGTTAA+TGG 0.292630 3_2:+82819948 Msa0430960:three_prime_UTR
GGTTGAATTAATGGCGGATT+TGG 0.298923 3_2:+82817594 Msa0430960:CDS
AAAGAGAGACACTCTGTTAC+AGG 0.308641 3_2:-82820118 None:intergenic
TTGGTGGAGATTGTGGAGGT+TGG 0.310557 3_2:+82817715 Msa0430960:CDS
AAGGCGTTGGATTCTGGTTT+CGG 0.334331 3_2:-82817014 None:intergenic
TGGAAGATGTGGAGCAATTC+CGG 0.336516 3_2:+82817444 Msa0430960:CDS
GAAGAGAATAAAGGAGCAAT+TGG 0.355932 3_2:+82817424 Msa0430960:CDS
TGAAGGTGCTATTCCTCCTT+TGG 0.356535 3_2:+82817822 Msa0430960:CDS
TTGTAAACTAACCTTGTCTT+TGG 0.375974 3_2:-82817875 None:intergenic
CAAGTATATATGTTGATTAG+AGG 0.381732 3_2:-82816870 None:intergenic
TGGTGAGCACAAAGGATATC+TGG 0.396238 3_2:-82819909 None:intergenic
CGGTTTAATAGCGCCGGCTT+TGG 0.400784 3_2:-82817188 None:intergenic
ATCATCACCGTCGCCGGGGA+TGG 0.404318 3_2:+82817225 Msa0430960:CDS
ATGTATCGCTGTTAGATTGT+TGG 0.419071 3_2:-82819939 None:intergenic
GTCTTTGGCGCGAATGGTAC+CGG 0.420372 3_2:-82817860 None:intergenic
TTCTGGTGCGATTAAACCTA+TGG 0.424118 3_2:+82817327 Msa0430960:CDS
AAAATTAGAGCGGTGAAAGC+TGG 0.424978 3_2:+82817556 Msa0430960:CDS
TGGTGGAGATTGTGGAGGTT+GGG 0.434537 3_2:+82817716 Msa0430960:CDS
GGTGGAGGAAGGAGGGATTC+CGG 0.437700 3_2:+82817690 Msa0430960:CDS
TCGAGAAGGTTCACCAATGC+CGG 0.444467 3_2:-82817463 None:intergenic
TCACAGACAGAAGAGAATAA+AGG 0.451466 3_2:+82817415 Msa0430960:CDS
TTCGGTTGTAGTGGTGAATT+CGG 0.452339 3_2:-82816996 None:intergenic
GGAAGCGAGGACGGCGTTGG+TGG 0.454845 3_2:+82817672 Msa0430960:CDS
TCCGGTGTTGGTGGAGATTG+TGG 0.459535 3_2:+82817708 Msa0430960:CDS
GAGCGTGTTGGTGACGGTTG+TGG 0.461714 3_2:+82817651 Msa0430960:CDS
TTGGTTTCTCTTTCTCAGTC+CGG 0.462112 3_2:+82817841 Msa0430960:CDS
ACAGTGGCATTGCTAGATCT+TGG 0.468286 3_2:-82819855 None:intergenic
AGCGAGGACGGCGTTGGTGG+AGG 0.468776 3_2:+82817675 Msa0430960:CDS
AACCTTGTCTTTGGCGCGAA+TGG 0.469160 3_2:-82817866 None:intergenic
GGAAGGAGGGATTCCGGTGT+TGG 0.479242 3_2:+82817696 Msa0430960:CDS
GAGTTCGTCGGAATAATTAT+CGG 0.485596 3_2:-82817053 None:intergenic
AGCTGGGATAATGAAGCCTT+TGG 0.485681 3_2:+82817573 Msa0430960:CDS
GCTGCATTCTCCTTCGCAGT+TGG 0.494828 3_2:-82817373 None:intergenic
AGACACTAATAGAGCTTCTA+CGG 0.497235 3_2:+82819829 Msa0430960:CDS
GGCGGATTTGGATTCGTACA+TGG 0.497248 3_2:+82817606 Msa0430960:CDS
GGTTTATGTTGATATTTATG+AGG 0.498706 3_2:-82819883 None:intergenic
GTCTTCAAAGCTTTCACCAT+AGG 0.500526 3_2:-82817343 None:intergenic
TATCGTAACGAATTACGTAA+TGG 0.507898 3_2:+82816907 Msa0430960:exon
CGGCGACGGTGATGATAAAA+GGG 0.510450 3_2:-82817218 None:intergenic
ACGGCGTTGGTGGAGGAAGG+AGG 0.512484 3_2:+82817682 Msa0430960:CDS
ATGTGGAGCAATTCCGGCAT+TGG 0.515544 3_2:+82817450 Msa0430960:CDS
AAATTAGAGCGGTGAAAGCT+GGG 0.520695 3_2:+82817557 Msa0430960:CDS
TTGGTGAACCTTCTCGAAAA+CGG 0.521459 3_2:+82817469 Msa0430960:CDS
AATGAAGATCGAGAATCAAC+CGG 0.523414 3_2:-82817081 None:intergenic
CGAGTAATGGTCTGCCGTGA+AGG 0.523826 3_2:+82817805 Msa0430960:CDS
GCAGTCGCTGAAAGCGGAAG+AGG 0.524507 3_2:-82816957 None:intergenic
TGTGGAAGCGAGGACGGCGT+TGG 0.525083 3_2:+82817669 Msa0430960:CDS
GACGGTTGTGGAAGCGAGGA+CGG 0.527350 3_2:+82817663 Msa0430960:CDS
CGTCTCAGAATCGCCAAAGC+CGG 0.529146 3_2:+82817175 Msa0430960:CDS
CGGCGTTGGTGGAGGAAGGA+GGG 0.530478 3_2:+82817683 Msa0430960:CDS
AAGGAGGAATAGCACCTTCA+CGG 0.530668 3_2:-82817819 None:intergenic
AAAGGAGCAATTGGAAGATG+TGG 0.538963 3_2:+82817433 Msa0430960:CDS
AGGACGGCGTTGGTGGAGGA+AGG 0.544626 3_2:+82817679 Msa0430960:CDS
AGGAGGGATTCCGGTGTTGG+TGG 0.550459 3_2:+82817699 Msa0430960:CDS
ATGGTGAAAGCTTTGAAGAC+AGG 0.551125 3_2:+82817346 Msa0430960:CDS
TGTGATGAGCGTGTTGGTGA+CGG 0.551202 3_2:+82817645 Msa0430960:CDS
TGCAGTTGAAGATCCATCCC+CGG 0.553429 3_2:-82817238 None:intergenic
TAGTATAAATTATGCTAGCT+TGG 0.553785 3_2:+82820047 Msa0430960:three_prime_UTR
TCCGCCATTAATTCAACCAA+AGG 0.559491 3_2:-82817589 None:intergenic
TGAAGATCCATCCCCGGCGA+CGG 0.564290 3_2:-82817232 None:intergenic
TCGAAAACGGAGGAATCCGC+GGG 0.567283 3_2:+82817482 Msa0430960:CDS
ACAAAGCTTCTTCGTTACAA+AGG 0.569110 3_2:-82820014 None:intergenic
TCCACAATCTCCACCAACAC+CGG 0.569993 3_2:-82817709 None:intergenic
TACCATTCGCGCCAAAGACA+AGG 0.571154 3_2:+82817864 Msa0430960:CDS
AATCAACGGTTTAATAGCGC+CGG 0.573314 3_2:-82817194 None:intergenic
CTCGAAAACGGAGGAATCCG+CGG 0.580066 3_2:+82817481 Msa0430960:CDS
GATTCTGGTTTCGGTTGTAG+TGG 0.584774 3_2:-82817005 None:intergenic
GAGCACAAAGGATATCTGGT+AGG 0.585677 3_2:-82819905 None:intergenic
GGTTGTAGTGGTGAATTCGG+TGG 0.586585 3_2:-82816993 None:intergenic
GCCTTTGGTTGAATTAATGG+CGG 0.592649 3_2:+82817588 Msa0430960:CDS
GAATATGAGTTATCGAGTAA+TGG 0.593225 3_2:+82817792 Msa0430960:CDS
CGGAAGCGCATGTAATGAGA+AGG 0.594684 3_2:-82817033 None:intergenic
GTTAGATTGTTGGAGTACAT+TGG 0.596753 3_2:-82819929 None:intergenic
ACTGTCGCAGTCGCTGAAAG+CGG 0.605550 3_2:-82816963 None:intergenic
CGCATGTAATGAGAAGGCGT+TGG 0.608908 3_2:-82817027 None:intergenic
GGTTGGGACGCAGAGACAGA+AGG 0.611749 3_2:+82817732 Msa0430960:CDS
AGCACAAAGGATATCTGGTA+GGG 0.612741 3_2:-82819904 None:intergenic
GTAACGAATTACGTAATGGA+AGG 0.615430 3_2:+82816911 Msa0430960:exon
ATTCCGACGAACTCATTCGC+CGG 0.620409 3_2:+82817062 Msa0430960:CDS
GACAGGCACTCCAACTGCGA+AGG 0.621740 3_2:+82817363 Msa0430960:CDS
GTTGATTAGAGGAAAAGTGA+TGG 0.624006 3_2:-82816859 None:intergenic
GACTGAGAAAGAGAAACCAA+AGG 0.629876 3_2:-82817838 None:intergenic
TATTTATGAGGCACGTACAG+TGG 0.637169 3_2:-82819871 None:intergenic
GATGATAAAAGGGAAATCAA+CGG 0.645026 3_2:-82817208 None:intergenic
GCAGAGACAGAAGGAAATTG+CGG 0.654422 3_2:+82817741 Msa0430960:CDS
CAACCGGCGAATGAGTTCGT+CGG 0.657343 3_2:-82817065 None:intergenic
GAGTACATTGGTGAGCACAA+AGG 0.660268 3_2:-82819917 None:intergenic
TGGTGACGGTTGTGGAAGCG+AGG 0.674684 3_2:+82817659 Msa0430960:CDS
GAGACAGAAGGAAATTGCGG+CGG 0.675465 3_2:+82817744 Msa0430960:CDS
GGTGTTGGTGGAGATTGTGG+AGG 0.676028 3_2:+82817711 Msa0430960:CDS
GAAAGAGAACAAAATTAGAG+CGG 0.684942 3_2:+82817546 Msa0430960:CDS
GGATTTGGATTCGTACATGG+TGG 0.694539 3_2:+82817609 Msa0430960:CDS
AGCGTATGTGATGAGCGTGT+TGG 0.695671 3_2:+82817639 Msa0430960:CDS
TGAGAAAGAGAAACCAAAGG+AGG 0.707414 3_2:-82817835 None:intergenic
TCTATATTATAAAGTGAACG+TGG 0.709631 3_2:-82816835 None:intergenic
GTGAACCTTCTCGAAAACGG+AGG 0.712449 3_2:+82817472 Msa0430960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATATTTTTTTTTGACA+TGG + chr3_2:82819685-82819704 Msa0430960:intron 10.0%
!!! AATATATTTTTTTTTGACAT+GGG + chr3_2:82819686-82819705 Msa0430960:intron 10.0%
!!! AAATTTTACTTTTACTGTAT+TGG + chr3_2:82818107-82818126 Msa0430960:intron 15.0%
!!! TTTCTTTTTTAAAGATTTGA+TGG + chr3_2:82818183-82818202 Msa0430960:intron 15.0%
!!! TTCTTTTTTAAAGATTTGAT+GGG + chr3_2:82818184-82818203 Msa0430960:intron 15.0%
!! AAAAGTAATTAAAACTCAAA+TGG + chr3_2:82818550-82818569 Msa0430960:intron 15.0%
!!! TAATAATATTCTTTTTTTGG+TGG + chr3_2:82818660-82818679 Msa0430960:intron 15.0%
!!! TCTAAATATTATTTTCCTAA+GGG + chr3_2:82818888-82818907 Msa0430960:intron 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - chr3_2:82818985-82819004 None:intergenic 15.0%
!! AAATTATCTTAATCAACTAT+TGG - chr3_2:82819122-82819141 None:intergenic 15.0%
!! AATAGTTGATTAAGATAATT+TGG + chr3_2:82819121-82819140 Msa0430960:intron 15.0%
!!! TTTACATCATTTTTTTTGAA+AGG - chr3_2:82819432-82819451 None:intergenic 15.0%
!!! TGATATTTTCTTTTCATCAA+TGG + chr3_2:82818147-82818166 Msa0430960:intron 20.0%
!!! TCTTTTTTAAAGATTTGATG+GGG + chr3_2:82818185-82818204 Msa0430960:intron 20.0%
!!! TTATAAAGTGTGTTTTATTG+AGG + chr3_2:82818385-82818404 Msa0430960:intron 20.0%
!! ATATGTAACGTATACATATA+TGG + chr3_2:82818574-82818593 Msa0430960:intron 20.0%
!! GACAATGTATAATATATTCA+AGG - chr3_2:82818707-82818726 None:intergenic 20.0%
!! ATTATGTAGAATTGATGTTT+GGG + chr3_2:82818863-82818882 Msa0430960:intron 20.0%
!!! GTCTAAATATTATTTTCCTA+AGG + chr3_2:82818887-82818906 Msa0430960:intron 20.0%
!! GTAACATAGTATTAATTGAA+GGG - chr3_2:82819059-82819078 None:intergenic 20.0%
!! TGTAACATAGTATTAATTGA+AGG - chr3_2:82819060-82819079 None:intergenic 20.0%
!! TGATTATGATAAACTTACAA+AGG - chr3_2:82819319-82819338 None:intergenic 20.0%
! TCTATATTATAAAGTGAACG+TGG - chr3_2:82816838-82816857 None:intergenic 25.0%
!! CAAGTATATATGTTGATTAG+AGG - chr3_2:82816873-82816892 None:intergenic 25.0%
!! TTTTTGTTGATCAATAGAAG+AGG - chr3_2:82817107-82817126 None:intergenic 25.0%
!!! ACGATTTTCTGATTTGTTTT+TGG - chr3_2:82817158-82817177 None:intergenic 25.0%
!!! ATTTTATACGGTGTTGAATA+AGG - chr3_2:82818466-82818485 None:intergenic 25.0%
! TATAATATATTCAAGGTCTG+AGG - chr3_2:82818700-82818719 None:intergenic 25.0%
! CATTATGTAGAATTGATGTT+TGG + chr3_2:82818862-82818881 Msa0430960:intron 25.0%
!! TATTATTTTCCTAAGGGTTA+AGG + chr3_2:82818894-82818913 Msa0430960:intron 25.0%
!!! AAACGACACTCATTTTAAAA+CGG - chr3_2:82818959-82818978 None:intergenic 25.0%
! GAAAATTGATCTACTCTAAA+TGG + chr3_2:82819271-82819290 Msa0430960:intron 25.0%
! GTCAAAAAAATTATGCAAAG+TGG - chr3_2:82819464-82819483 None:intergenic 25.0%
!!! CTTTGCATAATTTTTTTGAC+AGG + chr3_2:82819464-82819483 Msa0430960:intron 25.0%
!!! TATTTTGATTTCGTGTCTAA+TGG + chr3_2:82819731-82819750 Msa0430960:intron 25.0%
! GGTTTATGTTGATATTTATG+AGG - chr3_2:82819886-82819905 None:intergenic 25.0%
!!! TATACAAGTTGATGCTTTTA+GGG - chr3_2:82819990-82820009 None:intergenic 25.0%
!!! ATATACAAGTTGATGCTTTT+AGG - chr3_2:82819991-82820010 None:intergenic 25.0%
! TAGTATAAATTATGCTAGCT+TGG + chr3_2:82820047-82820066 Msa0430960:three_prime_UTR 25.0%
TATCGTAACGAATTACGTAA+TGG + chr3_2:82816907-82816926 Msa0430960:exon 30.0%
TCAACAAAAACAAGCTACAA+TGG + chr3_2:82817117-82817136 Msa0430960:CDS 30.0%
GATGATAAAAGGGAAATCAA+CGG - chr3_2:82817211-82817230 None:intergenic 30.0%
AAAGAGATTATCACTTCTTC+TGG + chr3_2:82817310-82817329 Msa0430960:CDS 30.0%
GAAAGAGAACAAAATTAGAG+CGG + chr3_2:82817546-82817565 Msa0430960:CDS 30.0%
GAATATGAGTTATCGAGTAA+TGG + chr3_2:82817792-82817811 Msa0430960:CDS 30.0%
TTGTAAACTAACCTTGTCTT+TGG - chr3_2:82817878-82817897 None:intergenic 30.0%
!! TTTAAAACGGAGAGAGTATT+TGG - chr3_2:82818946-82818965 None:intergenic 30.0%
!! TTCCTTAATTTGTGTGCAAT+TGG + chr3_2:82819178-82819197 Msa0430960:intron 30.0%
AACCAATTGCACACAAATTA+AGG - chr3_2:82819183-82819202 None:intergenic 30.0%
TAACAGCGATACATAGTTAA+TGG + chr3_2:82819948-82819967 Msa0430960:three_prime_UTR 30.0%
! TGAACGTGGAAAAGAGATTT+TGG - chr3_2:82816824-82816843 None:intergenic 35.0%
GTTGATTAGAGGAAAAGTGA+TGG - chr3_2:82816862-82816881 None:intergenic 35.0%
! GTAACGAATTACGTAATGGA+AGG + chr3_2:82816911-82816930 Msa0430960:exon 35.0%
GAGTTCGTCGGAATAATTAT+CGG - chr3_2:82817056-82817075 None:intergenic 35.0%
AATGAAGATCGAGAATCAAC+CGG - chr3_2:82817084-82817103 None:intergenic 35.0%
! TATTTTCATCGCATAGTGAG+AGG - chr3_2:82817291-82817310 None:intergenic 35.0%
TCACAGACAGAAGAGAATAA+AGG + chr3_2:82817415-82817434 Msa0430960:CDS 35.0%
GAAGAGAATAAAGGAGCAAT+TGG + chr3_2:82817424-82817443 Msa0430960:CDS 35.0%
GAAGCCTTTGGTTGAATTAA+TGG + chr3_2:82817585-82817604 Msa0430960:CDS 35.0%
TACAACTCTTCATGTTTACG+CGG + chr3_2:82817893-82817912 Msa0430960:intron 35.0%
TGCAATTTGCAATTGCAAGA+TGG - chr3_2:82817946-82817965 None:intergenic 35.0%
!!! ATTCTTTTTTTGGTGGTGTC+CGG + chr3_2:82818667-82818686 Msa0430960:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG + chr3_2:82819204-82819223 Msa0430960:intron 35.0%
! TTGTTGATTGTTCCTCTTCA+TGG + chr3_2:82819794-82819813 Msa0430960:intron 35.0%
! TGTTGATTGTTCCTCTTCAT+GGG + chr3_2:82819795-82819814 Msa0430960:intron 35.0%
AGACACTAATAGAGCTTCTA+CGG + chr3_2:82819829-82819848 Msa0430960:CDS 35.0%
GTTAGATTGTTGGAGTACAT+TGG - chr3_2:82819932-82819951 None:intergenic 35.0%
ATGTATCGCTGTTAGATTGT+TGG - chr3_2:82819942-82819961 None:intergenic 35.0%
ACAAAGCTTCTTCGTTACAA+AGG - chr3_2:82820017-82820036 None:intergenic 35.0%
!! CTTAGTATGCTTTGCTTTTC+TGG + chr3_2:82820078-82820097 Msa0430960:three_prime_UTR 35.0%
TTCGGTTGTAGTGGTGAATT+CGG - chr3_2:82816999-82817018 None:intergenic 40.0%
AATCAACGGTTTAATAGCGC+CGG - chr3_2:82817197-82817216 None:intergenic 40.0%
TTCTGGTGCGATTAAACCTA+TGG + chr3_2:82817327-82817346 Msa0430960:CDS 40.0%
GTCTTCAAAGCTTTCACCAT+AGG - chr3_2:82817346-82817365 None:intergenic 40.0%
!! ATGGTGAAAGCTTTGAAGAC+AGG + chr3_2:82817346-82817365 Msa0430960:CDS 40.0%
AAAGGAGCAATTGGAAGATG+TGG + chr3_2:82817433-82817452 Msa0430960:CDS 40.0%
TTGGTGAACCTTCTCGAAAA+CGG + chr3_2:82817469-82817488 Msa0430960:CDS 40.0%
AAAATTAGAGCGGTGAAAGC+TGG + chr3_2:82817556-82817575 Msa0430960:CDS 40.0%
AAATTAGAGCGGTGAAAGCT+GGG + chr3_2:82817557-82817576 Msa0430960:CDS 40.0%
GCCTTTGGTTGAATTAATGG+CGG + chr3_2:82817588-82817607 Msa0430960:CDS 40.0%
TCCGCCATTAATTCAACCAA+AGG - chr3_2:82817592-82817611 None:intergenic 40.0%
!! GGTTGAATTAATGGCGGATT+TGG + chr3_2:82817594-82817613 Msa0430960:CDS 40.0%
TGAGAAAGAGAAACCAAAGG+AGG - chr3_2:82817838-82817857 None:intergenic 40.0%
GACTGAGAAAGAGAAACCAA+AGG - chr3_2:82817841-82817860 None:intergenic 40.0%
TTGGTTTCTCTTTCTCAGTC+CGG + chr3_2:82817841-82817860 Msa0430960:CDS 40.0%
TGGAAGAATTACTGCTGACA+TGG - chr3_2:82817926-82817945 None:intergenic 40.0%
ATTTGGATGAGCACAATGCA+TGG - chr3_2:82818929-82818948 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr3_2:82819208-82819227 Msa0430960:intron 40.0%
TGATTTCGTGTCTAATGGTG+TGG + chr3_2:82819736-82819755 Msa0430960:intron 40.0%
GTTGATTGTTCCTCTTCATG+GGG + chr3_2:82819796-82819815 Msa0430960:intron 40.0%
TATTTATGAGGCACGTACAG+TGG - chr3_2:82819874-82819893 None:intergenic 40.0%
AGCACAAAGGATATCTGGTA+GGG - chr3_2:82819907-82819926 None:intergenic 40.0%
!!! CAAGTTGATGCTTTTAGGGT+GGG - chr3_2:82819986-82820005 None:intergenic 40.0%
!!! ACAAGTTGATGCTTTTAGGG+TGG - chr3_2:82819987-82820006 None:intergenic 40.0%
!! GATTCTGGTTTCGGTTGTAG+TGG - chr3_2:82817008-82817027 None:intergenic 45.0%
!! AAGGCGTTGGATTCTGGTTT+CGG - chr3_2:82817017-82817036 None:intergenic 45.0%
!! AATGAGAAGGCGTTGGATTC+TGG - chr3_2:82817023-82817042 None:intergenic 45.0%
TGGAAGATGTGGAGCAATTC+CGG + chr3_2:82817444-82817463 Msa0430960:CDS 45.0%
!!! TTGACGCATCTTTTTTCCCG+CGG - chr3_2:82817501-82817520 None:intergenic 45.0%
CGGGAAAAAAGATGCGTCAA+CGG + chr3_2:82817501-82817520 Msa0430960:CDS 45.0%
AGCTGGGATAATGAAGCCTT+TGG + chr3_2:82817573-82817592 Msa0430960:CDS 45.0%
GGATTTGGATTCGTACATGG+TGG + chr3_2:82817609-82817628 Msa0430960:CDS 45.0%
GCAGAGACAGAAGGAAATTG+CGG + chr3_2:82817741-82817760 Msa0430960:CDS 45.0%
AAGGAGGAATAGCACCTTCA+CGG - chr3_2:82817822-82817841 None:intergenic 45.0%
!! TGAAGGTGCTATTCCTCCTT+TGG + chr3_2:82817822-82817841 Msa0430960:CDS 45.0%
!!! TTTTTGGTGGTGTCCGGATT+CGG + chr3_2:82818673-82818692 Msa0430960:intron 45.0%
TTACCAACTGAGCTAGCTCA+CGG + chr3_2:82818728-82818747 Msa0430960:intron 45.0%
AAGGACATGCCTTAACCCTT+AGG - chr3_2:82818906-82818925 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + chr3_2:82819207-82819226 Msa0430960:intron 45.0%
ACAGTGGCATTGCTAGATCT+TGG - chr3_2:82819858-82819877 None:intergenic 45.0%
GAGCACAAAGGATATCTGGT+AGG - chr3_2:82819908-82819927 None:intergenic 45.0%
TGGTGAGCACAAAGGATATC+TGG - chr3_2:82819912-82819931 None:intergenic 45.0%
GAGTACATTGGTGAGCACAA+AGG - chr3_2:82819920-82819939 None:intergenic 45.0%
!!! TATAAATAACATATTTTATA+CGG - chr3_2:82818478-82818497 None:intergenic 5.0%
!!! TTTTAATAATATTCTTTTTT+TGG + chr3_2:82818657-82818676 Msa0430960:intron 5.0%
!! ATTAAAGAAAAAATAATATT+TGG - chr3_2:82819089-82819108 None:intergenic 5.0%
!! AAATAAATAAATCTAATAAA+AGG - chr3_2:82819371-82819390 None:intergenic 5.0%
GGTTGTAGTGGTGAATTCGG+TGG - chr3_2:82816996-82817015 None:intergenic 50.0%
! CGCATGTAATGAGAAGGCGT+TGG - chr3_2:82817030-82817049 None:intergenic 50.0%
! CGGAAGCGCATGTAATGAGA+AGG - chr3_2:82817036-82817055 None:intergenic 50.0%
ATTCCGACGAACTCATTCGC+CGG + chr3_2:82817062-82817081 Msa0430960:CDS 50.0%
CGGCGACGGTGATGATAAAA+GGG - chr3_2:82817221-82817240 None:intergenic 50.0%
! CCTTTTATCATCACCGTCGC+CGG + chr3_2:82817219-82817238 Msa0430960:CDS 50.0%
! CTTTTATCATCACCGTCGCC+GGG + chr3_2:82817220-82817239 Msa0430960:CDS 50.0%
! TTTTATCATCACCGTCGCCG+GGG + chr3_2:82817221-82817240 Msa0430960:CDS 50.0%
TGCAGTTGAAGATCCATCCC+CGG - chr3_2:82817241-82817260 None:intergenic 50.0%
ATGTGGAGCAATTCCGGCAT+TGG + chr3_2:82817450-82817469 Msa0430960:CDS 50.0%
TCGAGAAGGTTCACCAATGC+CGG - chr3_2:82817466-82817485 None:intergenic 50.0%
GTGAACCTTCTCGAAAACGG+AGG + chr3_2:82817472-82817491 Msa0430960:CDS 50.0%
!! GGATTCCTCCGTTTTCGAGA+AGG - chr3_2:82817480-82817499 None:intergenic 50.0%
! GGCGGATTTGGATTCGTACA+TGG + chr3_2:82817606-82817625 Msa0430960:CDS 50.0%
AGCGTATGTGATGAGCGTGT+TGG + chr3_2:82817639-82817658 Msa0430960:CDS 50.0%
! TGTGATGAGCGTGTTGGTGA+CGG + chr3_2:82817645-82817664 Msa0430960:CDS 50.0%
TCCACAATCTCCACCAACAC+CGG - chr3_2:82817712-82817731 None:intergenic 50.0%
TTGGTGGAGATTGTGGAGGT+TGG + chr3_2:82817715-82817734 Msa0430960:CDS 50.0%
TGGTGGAGATTGTGGAGGTT+GGG + chr3_2:82817716-82817735 Msa0430960:CDS 50.0%
GAGACAGAAGGAAATTGCGG+CGG + chr3_2:82817744-82817763 Msa0430960:CDS 50.0%
TACCATTCGCGCCAAAGACA+AGG + chr3_2:82817864-82817883 Msa0430960:CDS 50.0%
AACCTTGTCTTTGGCGCGAA+TGG - chr3_2:82817869-82817888 None:intergenic 50.0%
! GTGAGCTAGCTCAGTTGGTA+AGG - chr3_2:82818729-82818748 None:intergenic 50.0%
GGATGAGCACAATGCATGGA+AGG - chr3_2:82818925-82818944 None:intergenic 50.0%
ACTGTCGCAGTCGCTGAAAG+CGG - chr3_2:82816966-82816985 None:intergenic 55.0%
CAACCGGCGAATGAGTTCGT+CGG - chr3_2:82817068-82817087 None:intergenic 55.0%
CGTCTCAGAATCGCCAAAGC+CGG + chr3_2:82817175-82817194 Msa0430960:CDS 55.0%
!! CGGTTTAATAGCGCCGGCTT+TGG - chr3_2:82817191-82817210 None:intergenic 55.0%
CCGGCGACGGTGATGATAAA+AGG - chr3_2:82817222-82817241 None:intergenic 55.0%
GCTGCATTCTCCTTCGCAGT+TGG - chr3_2:82817376-82817395 None:intergenic 55.0%
CTCGAAAACGGAGGAATCCG+CGG + chr3_2:82817481-82817500 Msa0430960:CDS 55.0%
TCGAAAACGGAGGAATCCGC+GGG + chr3_2:82817482-82817501 Msa0430960:CDS 55.0%
!! TCCGGTGTTGGTGGAGATTG+TGG + chr3_2:82817708-82817727 Msa0430960:CDS 55.0%
!! GGTGTTGGTGGAGATTGTGG+AGG + chr3_2:82817711-82817730 Msa0430960:CDS 55.0%
CGAGTAATGGTCTGCCGTGA+AGG + chr3_2:82817805-82817824 Msa0430960:CDS 55.0%
GTCTTTGGCGCGAATGGTAC+CGG - chr3_2:82817863-82817882 None:intergenic 55.0%
! CAAGGTCTGAGGTCCGAATC+CGG - chr3_2:82818689-82818708 None:intergenic 55.0%
TCTCCGTGAGCTAGCTCAGT+TGG - chr3_2:82818734-82818753 None:intergenic 55.0%
GTTCCTCTTCATGGGGACAC+AGG + chr3_2:82819803-82819822 Msa0430960:intron 55.0%
CTACCTGTGTCCCCATGAAG+AGG - chr3_2:82819809-82819828 None:intergenic 55.0%
GCAGTCGCTGAAAGCGGAAG+AGG - chr3_2:82816960-82816979 None:intergenic 60.0%
TGAAGATCCATCCCCGGCGA+CGG - chr3_2:82817235-82817254 None:intergenic 60.0%
GACAGGCACTCCAACTGCGA+AGG + chr3_2:82817363-82817382 Msa0430960:CDS 60.0%
!! GAGCGTGTTGGTGACGGTTG+TGG + chr3_2:82817651-82817670 Msa0430960:CDS 60.0%
!! TGGTGACGGTTGTGGAAGCG+AGG + chr3_2:82817659-82817678 Msa0430960:CDS 60.0%
GACGGTTGTGGAAGCGAGGA+CGG + chr3_2:82817663-82817682 Msa0430960:CDS 60.0%
GGTGGAGGAAGGAGGGATTC+CGG + chr3_2:82817690-82817709 Msa0430960:CDS 60.0%
GGAAGGAGGGATTCCGGTGT+TGG + chr3_2:82817696-82817715 Msa0430960:CDS 60.0%
! AGGAGGGATTCCGGTGTTGG+TGG + chr3_2:82817699-82817718 Msa0430960:CDS 60.0%
GGTTGGGACGCAGAGACAGA+AGG + chr3_2:82817732-82817751 Msa0430960:CDS 60.0%
ATCATCACCGTCGCCGGGGA+TGG + chr3_2:82817225-82817244 Msa0430960:CDS 65.0%
TGTGGAAGCGAGGACGGCGT+TGG + chr3_2:82817669-82817688 Msa0430960:CDS 65.0%
!! AGGACGGCGTTGGTGGAGGA+AGG + chr3_2:82817679-82817698 Msa0430960:CDS 65.0%
!! ACGGCGTTGGTGGAGGAAGG+AGG + chr3_2:82817682-82817701 Msa0430960:CDS 65.0%
!! CGGCGTTGGTGGAGGAAGGA+GGG + chr3_2:82817683-82817702 Msa0430960:CDS 65.0%
! GGAAGCGAGGACGGCGTTGG+TGG + chr3_2:82817672-82817691 Msa0430960:CDS 70.0%
!! AGCGAGGACGGCGTTGGTGG+AGG + chr3_2:82817675-82817694 Msa0430960:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 82816821 82820133 82816821 ID=Msa0430960;Name=Msa0430960
chr3_2 mRNA 82816821 82820133 82816821 ID=Msa0430960-mRNA-1;Parent=Msa0430960;Name=Msa0430960-mRNA-1;_AED=0.02;_eAED=0.02;_QI=105|1|1|1|1|1|2|243|341
chr3_2 exon 82816821 82817885 82816821 ID=Msa0430960-mRNA-1:exon:23060;Parent=Msa0430960-mRNA-1
chr3_2 exon 82819825 82820133 82819825 ID=Msa0430960-mRNA-1:exon:23061;Parent=Msa0430960-mRNA-1
chr3_2 five_prime_UTR 82816821 82816925 82816821 ID=Msa0430960-mRNA-1:five_prime_utr;Parent=Msa0430960-mRNA-1
chr3_2 CDS 82816926 82817885 82816926 ID=Msa0430960-mRNA-1:cds;Parent=Msa0430960-mRNA-1
chr3_2 CDS 82819825 82819890 82819825 ID=Msa0430960-mRNA-1:cds;Parent=Msa0430960-mRNA-1
chr3_2 three_prime_UTR 82819891 82820133 82819891 ID=Msa0430960-mRNA-1:three_prime_utr;Parent=Msa0430960-mRNA-1
Gene Sequence

>Msa0430960

ATGGAAGGAACTTTCAATTTCAACGATAATTCCTCTTCCGCTTTCAGCGACTGCGACAGTGACAGATCCACCGAATTCACCACTACAACCGAAACCAGAATCCAACGCCTTCTCATTACATGCGCTTCCGATAATTATTCCGACGAACTCATTCGCCGGTTGATTCTCGATCTTCATTCCTCTTCTATTGATCAACAAAAACAAGCTACAATGGAAATCAGATTACTCGCCAAAAACAAATCAGAAAATCGTCTCAGAATCGCCAAAGCCGGCGCTATTAAACCGTTGATTTCCCTTTTATCATCACCGTCGCCGGGGATGGATCTTCAACTGCAAGAATACGTCGTCACAGCGATTTTAAACCTCTCACTATGCGATGAAAATAAAGAGATTATCACTTCTTCTGGTGCGATTAAACCTATGGTGAAAGCTTTGAAGACAGGCACTCCAACTGCGAAGGAGAATGCAGCATGTGCTCTGCTTCGTCTCTCACAGACAGAAGAGAATAAAGGAGCAATTGGAAGATGTGGAGCAATTCCGGCATTGGTGAACCTTCTCGAAAACGGAGGAATCCGCGGGAAAAAAGATGCGTCAACGGCGTTATACACGTTGTGTTCTGTGAAAGAGAACAAAATTAGAGCGGTGAAAGCTGGGATAATGAAGCCTTTGGTTGAATTAATGGCGGATTTGGATTCGTACATGGTGGATAAGTCAGCGTATGTGATGAGCGTGTTGGTGACGGTTGTGGAAGCGAGGACGGCGTTGGTGGAGGAAGGAGGGATTCCGGTGTTGGTGGAGATTGTGGAGGTTGGGACGCAGAGACAGAAGGAAATTGCGGCGGCGATTTTGTTGAAGATTTGTGAAGAGAATATGAGTTATCGAGTAATGGTCTGCCGTGAAGGTGCTATTCCTCCTTTGGTTTCTCTTTCTCAGTCCGGTACCATTCGCGCCAAAGACAAGGTAGAGACACTAATAGAGCTTCTACGGCAACCAAGATCTAGCAATGCCACTGTACGTGCCTCATAA
Protein sequence

>Msa0430960

MEGTFNFNDNSSSAFSDCDSDRSTEFTTTTETRIQRLLITCASDNYSDELIRRLILDLHSSSIDQQKQATMEIRLLAKNKSENRLRIAKAGAIKPLISLLSSPSPGMDLQLQEYVVTAILNLSLCDENKEIITSSGAIKPMVKALKTGTPTAKENAACALLRLSQTEENKGAIGRCGAIPALVNLLENGGIRGKKDASTALYTLCSVKENKIRAVKAGIMKPLVELMADLDSYMVDKSAYVMSVLVTVVEARTALVEEGGIPVLVEIVEVGTQRQKEIAAAILLKICEENMSYRVMVCREGAIPPLVSLSQSGTIRAKDKVETLIELLRQPRSSNATVRAS*