AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0431130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431130 MtrunA17_Chr3g0134941 95.652 184 7 1 1 183 1 184 2.96e-126 352
Msa0431130 MtrunA17_Chr1g0152381 50.943 159 61 5 1 149 1 152 1.97e-34 119
Msa0431130 MtrunA17_Chr5g0393821 38.415 164 87 6 7 164 6 161 6.98e-15 69.3
Msa0431130 MtrunA17_Chr1g0207011 35.152 165 98 3 1 156 1 165 1.76e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431130 AT4G33800.2 34.857 175 77 6 11 164 6 164 3.49e-12 62.0
Msa0431130 AT4G33800.1 34.857 175 77 6 11 164 6 164 3.49e-12 62.0
Msa0431130 AT1G58460.1 34.673 199 91 10 1 183 1 176 3.90e-11 59.3

Find 56 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACCTTATTGTGTAAAT+TGG 0.134932 3_2:+83116439 Msa0431130:CDS
CCAGAAGCATTCAAGTTTCT+TGG 0.140446 3_2:+83116869 Msa0431130:CDS
TAGACAAGTGTTAGTTAAAA+TGG 0.155616 3_2:+83116182 Msa0431130:exon
GATGATATATATATAGATTT+TGG 0.215906 3_2:-83116127 None:intergenic
ACAGTGACATATTGTTATTT+TGG 0.231001 3_2:-83117452 None:intergenic
GAATTCCAATTTACACAATA+AGG 0.257445 3_2:-83116444 None:intergenic
CCAAGAAACTTGAATGCTTC+TGG 0.269813 3_2:-83116869 None:intergenic
AAAGTCAATTTCTCAGAAAA+TGG 0.272188 3_2:+83116672 Msa0431130:CDS
ATAAAGAAGGAACAGTTCTT+TGG 0.278886 3_2:+83117144 Msa0431130:three_prime_UTR
ATGGTTTCTGATGCTTCTTC+TGG 0.283239 3_2:+83116381 Msa0431130:CDS
CATGATGTCAACTAAGAAAA+TGG 0.287865 3_2:-83117253 None:intergenic
ACCTTGGGATAGTTAAGAAC+AGG 0.357740 3_2:-83116555 None:intergenic
TACAATAGTGAAAGTGAATC+TGG 0.379261 3_2:+83116225 Msa0431130:CDS
TTTCTGAAGAACATTTCAAT+AGG 0.386107 3_2:+83116277 Msa0431130:CDS
CAAGTGTATATTTAGTTTGT+CGG 0.388292 3_2:+83117413 Msa0431130:three_prime_UTR
AAAAGAAACTCTAGTTCTAT+AGG 0.414355 3_2:+83116053 Msa0431130:five_prime_UTR
TGTAGGAAAAGGAGCAAGAA+TGG 0.424432 3_2:+83116320 Msa0431130:CDS
AATTTACACAATAAGGTTGT+TGG 0.428691 3_2:-83116437 None:intergenic
GATGAAGTAAACATAATGAT+AGG 0.453876 3_2:-83117347 None:intergenic
GTCCCACTGCCAATGCAGTT+TGG 0.454243 3_2:+83117199 Msa0431130:three_prime_UTR
GTTCTTGTTGCAGAGAAAGA+AGG 0.454355 3_2:-83116723 None:intergenic
TGCGTCAGAAGCTAAAAGTA+AGG 0.464184 3_2:+83117075 Msa0431130:three_prime_UTR
GAACATTTCAATAGGAAAAG+TGG 0.473979 3_2:+83116285 Msa0431130:CDS
CAATAGGAAAAGTGGGAAAG+TGG 0.474045 3_2:+83116293 Msa0431130:CDS
CATTCAAGTTTCTTGGATGG+TGG 0.474914 3_2:+83116876 Msa0431130:CDS
CTAGTGTTGTAGAGTGGTAA+AGG 0.492301 3_2:-83116077 None:intergenic
GTTCCAAACTGCATTGGCAG+TGG 0.494977 3_2:-83117202 None:intergenic
AAAGTGGATTATGTAGGAAA+AGG 0.502120 3_2:+83116309 Msa0431130:CDS
TTCTTGTTGCAGAGAAAGAA+GGG 0.506519 3_2:-83116722 None:intergenic
GTTTGGAACAACGTAAGAAC+AGG 0.508021 3_2:+83117216 Msa0431130:three_prime_UTR
AACATTTCAATAGGAAAAGT+GGG 0.508806 3_2:+83116286 Msa0431130:CDS
TAATGATAGGCAGAATTTGA+AGG 0.517062 3_2:-83117334 None:intergenic
TACGTTGTTCCAAACTGCAT+TGG 0.529331 3_2:-83117208 None:intergenic
AATGATAGGCAGAATTTGAA+GGG 0.538940 3_2:-83117333 None:intergenic
TGGTGGAAAACAAGCTTCAA+AGG 0.539652 3_2:+83116893 Msa0431130:CDS
AAGCATTCAAGTTTCTTGGA+TGG 0.545383 3_2:+83116873 Msa0431130:CDS
CCCTGTTCTTAACTATCCCA+AGG 0.552465 3_2:+83116554 Msa0431130:CDS
AGTGGGAAAGTGGATTATGT+AGG 0.561242 3_2:+83116303 Msa0431130:CDS
AGAAGTGTAAAGAATATGGT+AGG 0.566325 3_2:+83116490 Msa0431130:CDS
GGAAGAAGAAGATTTATCCA+TGG 0.566800 3_2:+83116362 Msa0431130:CDS
TTCCAAACTGCATTGGCAGT+GGG 0.575619 3_2:-83117201 None:intergenic
ATAGTGAAAGTGAATCTGGT+TGG 0.584733 3_2:+83116229 Msa0431130:CDS
AGAAGAAGCATCAGAAACCA+TGG 0.595770 3_2:-83116379 None:intergenic
CCTTGGGATAGTTAAGAACA+GGG 0.598514 3_2:-83116554 None:intergenic
GCGTCAGAAGCTAAAAGTAA+GGG 0.607870 3_2:+83117076 Msa0431130:three_prime_UTR
TTTCTCAGAAAATGGAGCAG+TGG 0.613686 3_2:+83116680 Msa0431130:CDS
TTTCTCTGCAACAAGAACAA+AGG 0.615674 3_2:+83116728 Msa0431130:CDS
TCTTGTTGCAGAGAAAGAAG+GGG 0.652031 3_2:-83116721 None:intergenic
AAACAAGCTTCAAAGGAACC+AGG 0.657474 3_2:+83116900 Msa0431130:CDS
TCCTCTACATGATAGTACTG+TGG 0.658500 3_2:-83116408 None:intergenic
GAACTAGCAGTATCATCAAG+AGG 0.659139 3_2:-83116531 None:intergenic
TGAAAACTAGTGTTGTAGAG+TGG 0.666228 3_2:-83116083 None:intergenic
ATGATAGGCAGAATTTGAAG+GGG 0.666686 3_2:-83117332 None:intergenic
TCCACAGTACTATCATGTAG+AGG 0.668253 3_2:+83116407 Msa0431130:CDS
TCTACATGATAGTACTGTGG+AGG 0.671420 3_2:-83116405 None:intergenic
CTTGGGATAGTTAAGAACAG+GGG 0.715448 3_2:-83116553 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATATAGATTTTGGTA+AGG - chr3_2:83116125-83116144 None:intergenic 15.0%
!!! GATGATATATATATAGATTT+TGG - chr3_2:83116130-83116149 None:intergenic 15.0%
!!! TTATAACTAATTTTATTTGG+AGG + chr3_2:83116614-83116633 Msa0431130:intron 15.0%
!! ATTAATTATGTATATATACC+TGG - chr3_2:83116921-83116940 None:intergenic 15.0%
!! AAAAATAGAGAAAACAAAAA+GGG - chr3_2:83117373-83117392 None:intergenic 15.0%
!! AAAAAATAGAGAAAACAAAA+AGG - chr3_2:83117374-83117393 None:intergenic 15.0%
!!! TTTGTTTTCTCTATTTTTTT+AGG + chr3_2:83117375-83117394 Msa0431130:three_prime_UTR 15.0%
!!! TATATATATAGATTTTGGTA+AGG - chr3_2:83116125-83116144 None:intergenic 15.0%
!!! GATGATATATATATAGATTT+TGG - chr3_2:83116130-83116149 None:intergenic 15.0%
!!! TTATAACTAATTTTATTTGG+AGG + chr3_2:83116614-83116633 Msa0431130:intron 15.0%
!! ATTAATTATGTATATATACC+TGG - chr3_2:83116921-83116940 None:intergenic 15.0%
!! AAAAATAGAGAAAACAAAAA+GGG - chr3_2:83117373-83117392 None:intergenic 15.0%
!! AAAAAATAGAGAAAACAAAA+AGG - chr3_2:83117374-83117393 None:intergenic 15.0%
!!! TTTGTTTTCTCTATTTTTTT+AGG + chr3_2:83117375-83117394 Msa0431130:three_prime_UTR 15.0%
!!! ATATTCTTTACACTTCTTTT+TGG - chr3_2:83116487-83116506 None:intergenic 20.0%
!!! AAAAAGAAGTGTAAAGAATA+TGG + chr3_2:83116486-83116505 Msa0431130:CDS 20.0%
!! ATGCTTATTAGTTATTACAA+TGG + chr3_2:83116787-83116806 Msa0431130:intron 20.0%
!! TTGTTTATGTCTTGATTATA+AGG + chr3_2:83117002-83117021 Msa0431130:intron 20.0%
!! AAGTGATGTAAAAATAAAGA+AGG + chr3_2:83117131-83117150 Msa0431130:three_prime_UTR 20.0%
!! AAAATAGAGAAAACAAAAAG+GGG - chr3_2:83117372-83117391 None:intergenic 20.0%
!!! CTAAATATACACTTGATTTT+TGG - chr3_2:83117408-83117427 None:intergenic 20.0%
!!! ATATTCTTTACACTTCTTTT+TGG - chr3_2:83116487-83116506 None:intergenic 20.0%
!!! AAAAAGAAGTGTAAAGAATA+TGG + chr3_2:83116486-83116505 Msa0431130:CDS 20.0%
!! ATGCTTATTAGTTATTACAA+TGG + chr3_2:83116787-83116806 Msa0431130:intron 20.0%
!! TTGTTTATGTCTTGATTATA+AGG + chr3_2:83117002-83117021 Msa0431130:intron 20.0%
!! AAGTGATGTAAAAATAAAGA+AGG + chr3_2:83117131-83117150 Msa0431130:three_prime_UTR 20.0%
!! AAAATAGAGAAAACAAAAAG+GGG - chr3_2:83117372-83117391 None:intergenic 20.0%
!!! CTAAATATACACTTGATTTT+TGG - chr3_2:83117408-83117427 None:intergenic 20.0%
!!! AAAAGAAACTCTAGTTCTAT+AGG + chr3_2:83116053-83116072 Msa0431130:five_prime_UTR 25.0%
! TAGACAAGTGTTAGTTAAAA+TGG + chr3_2:83116182-83116201 Msa0431130:exon 25.0%
!! TTTCTGAAGAACATTTCAAT+AGG + chr3_2:83116277-83116296 Msa0431130:CDS 25.0%
! AACATTTCAATAGGAAAAGT+GGG + chr3_2:83116286-83116305 Msa0431130:CDS 25.0%
! AATTTACACAATAAGGTTGT+TGG - chr3_2:83116440-83116459 None:intergenic 25.0%
! AACAACCTTATTGTGTAAAT+TGG + chr3_2:83116439-83116458 Msa0431130:CDS 25.0%
! GAATTCCAATTTACACAATA+AGG - chr3_2:83116447-83116466 None:intergenic 25.0%
! AAAGTCAATTTCTCAGAAAA+TGG + chr3_2:83116672-83116691 Msa0431130:CDS 25.0%
! AACTAATAAGCATAATGTGT+TGG - chr3_2:83116780-83116799 None:intergenic 25.0%
!!! TGAATGTTTTAAGCTAATGA+AGG + chr3_2:83116978-83116997 Msa0431130:intron 25.0%
! TTTATGTCTTGATTATAAGG+TGG + chr3_2:83117005-83117024 Msa0431130:intron 25.0%
!!! TGATTATAAGGTGGTTTTAA+TGG + chr3_2:83117014-83117033 Msa0431130:intron 25.0%
! GATGAAGTAAACATAATGAT+AGG - chr3_2:83117350-83117369 None:intergenic 25.0%
!!! CAAGTGTATATTTAGTTTGT+CGG + chr3_2:83117413-83117432 Msa0431130:three_prime_UTR 25.0%
!!! ACAGTGACATATTGTTATTT+TGG - chr3_2:83117455-83117474 None:intergenic 25.0%
!!! TCTTAATGTGTTTGTTTTCA+AGG - chr3_2:83116007-83116026 None:intergenic 25.0%
!!! AAAAGAAACTCTAGTTCTAT+AGG + chr3_2:83116053-83116072 Msa0431130:five_prime_UTR 25.0%
! TAGACAAGTGTTAGTTAAAA+TGG + chr3_2:83116182-83116201 Msa0431130:exon 25.0%
!! TTTCTGAAGAACATTTCAAT+AGG + chr3_2:83116277-83116296 Msa0431130:CDS 25.0%
! AACATTTCAATAGGAAAAGT+GGG + chr3_2:83116286-83116305 Msa0431130:CDS 25.0%
! AATTTACACAATAAGGTTGT+TGG - chr3_2:83116440-83116459 None:intergenic 25.0%
! AACAACCTTATTGTGTAAAT+TGG + chr3_2:83116439-83116458 Msa0431130:CDS 25.0%
! GAATTCCAATTTACACAATA+AGG - chr3_2:83116447-83116466 None:intergenic 25.0%
! AAAGTCAATTTCTCAGAAAA+TGG + chr3_2:83116672-83116691 Msa0431130:CDS 25.0%
! AACTAATAAGCATAATGTGT+TGG - chr3_2:83116780-83116799 None:intergenic 25.0%
!!! TGAATGTTTTAAGCTAATGA+AGG + chr3_2:83116978-83116997 Msa0431130:intron 25.0%
! TTTATGTCTTGATTATAAGG+TGG + chr3_2:83117005-83117024 Msa0431130:intron 25.0%
!!! TGATTATAAGGTGGTTTTAA+TGG + chr3_2:83117014-83117033 Msa0431130:intron 25.0%
! GATGAAGTAAACATAATGAT+AGG - chr3_2:83117350-83117369 None:intergenic 25.0%
!!! CAAGTGTATATTTAGTTTGT+CGG + chr3_2:83117413-83117432 Msa0431130:three_prime_UTR 25.0%
!!! ACAGTGACATATTGTTATTT+TGG - chr3_2:83117455-83117474 None:intergenic 25.0%
TACAATAGTGAAAGTGAATC+TGG + chr3_2:83116225-83116244 Msa0431130:CDS 30.0%
GTTCTTCAGAAAAAGATGAA+TGG - chr3_2:83116269-83116288 None:intergenic 30.0%
GAACATTTCAATAGGAAAAG+TGG + chr3_2:83116285-83116304 Msa0431130:CDS 30.0%
!! AAAGTGGATTATGTAGGAAA+AGG + chr3_2:83116309-83116328 Msa0431130:CDS 30.0%
! ACACTTCTTTTTGGATTCTT+TGG - chr3_2:83116478-83116497 None:intergenic 30.0%
! AGAAGTGTAAAGAATATGGT+AGG + chr3_2:83116490-83116509 Msa0431130:CDS 30.0%
TAAGCATAATGTGTTGGATT+TGG - chr3_2:83116774-83116793 None:intergenic 30.0%
ATAAAGAAGGAACAGTTCTT+TGG + chr3_2:83117144-83117163 Msa0431130:three_prime_UTR 30.0%
CATGATGTCAACTAAGAAAA+TGG - chr3_2:83117256-83117275 None:intergenic 30.0%
! CATTTTCTTAGTTGACATCA+TGG + chr3_2:83117254-83117273 Msa0431130:three_prime_UTR 30.0%
! CATGTCTTTTTGAATCTCTT+AGG + chr3_2:83117281-83117300 Msa0431130:three_prime_UTR 30.0%
AATGATAGGCAGAATTTGAA+GGG - chr3_2:83117336-83117355 None:intergenic 30.0%
TAATGATAGGCAGAATTTGA+AGG - chr3_2:83117337-83117356 None:intergenic 30.0%
TACAATAGTGAAAGTGAATC+TGG + chr3_2:83116225-83116244 Msa0431130:CDS 30.0%
GTTCTTCAGAAAAAGATGAA+TGG - chr3_2:83116269-83116288 None:intergenic 30.0%
GAACATTTCAATAGGAAAAG+TGG + chr3_2:83116285-83116304 Msa0431130:CDS 30.0%
!! AAAGTGGATTATGTAGGAAA+AGG + chr3_2:83116309-83116328 Msa0431130:CDS 30.0%
! ACACTTCTTTTTGGATTCTT+TGG - chr3_2:83116478-83116497 None:intergenic 30.0%
! AGAAGTGTAAAGAATATGGT+AGG + chr3_2:83116490-83116509 Msa0431130:CDS 30.0%
TAAGCATAATGTGTTGGATT+TGG - chr3_2:83116774-83116793 None:intergenic 30.0%
ATAAAGAAGGAACAGTTCTT+TGG + chr3_2:83117144-83117163 Msa0431130:three_prime_UTR 30.0%
CATGATGTCAACTAAGAAAA+TGG - chr3_2:83117256-83117275 None:intergenic 30.0%
! CATTTTCTTAGTTGACATCA+TGG + chr3_2:83117254-83117273 Msa0431130:three_prime_UTR 30.0%
! CATGTCTTTTTGAATCTCTT+AGG + chr3_2:83117281-83117300 Msa0431130:three_prime_UTR 30.0%
AATGATAGGCAGAATTTGAA+GGG - chr3_2:83117336-83117355 None:intergenic 30.0%
TAATGATAGGCAGAATTTGA+AGG - chr3_2:83117337-83117356 None:intergenic 30.0%
TGAAAACTAGTGTTGTAGAG+TGG - chr3_2:83116086-83116105 None:intergenic 35.0%
ATAGTGAAAGTGAATCTGGT+TGG + chr3_2:83116229-83116248 Msa0431130:CDS 35.0%
AATGGAAGAAGAAGAAGAAG+AGG + chr3_2:83116338-83116357 Msa0431130:CDS 35.0%
GGAAGAAGAAGATTTATCCA+TGG + chr3_2:83116362-83116381 Msa0431130:CDS 35.0%
!! TTAGCTTTGTGAGTTACCTT+GGG - chr3_2:83116573-83116592 None:intergenic 35.0%
!! ATTAGCTTTGTGAGTTACCT+TGG - chr3_2:83116574-83116593 None:intergenic 35.0%
TTCTTGTTGCAGAGAAAGAA+GGG - chr3_2:83116725-83116744 None:intergenic 35.0%
TTTCTCTGCAACAAGAACAA+AGG + chr3_2:83116728-83116747 Msa0431130:CDS 35.0%
! AAGCATTCAAGTTTCTTGGA+TGG + chr3_2:83116873-83116892 Msa0431130:CDS 35.0%
!! AAAAAGATGCAAGGTGTTGA+AGG + chr3_2:83117105-83117124 Msa0431130:three_prime_UTR 35.0%
ATGATAGGCAGAATTTGAAG+GGG - chr3_2:83117335-83117354 None:intergenic 35.0%
TGAAAACTAGTGTTGTAGAG+TGG - chr3_2:83116086-83116105 None:intergenic 35.0%
ATAGTGAAAGTGAATCTGGT+TGG + chr3_2:83116229-83116248 Msa0431130:CDS 35.0%
AATGGAAGAAGAAGAAGAAG+AGG + chr3_2:83116338-83116357 Msa0431130:CDS 35.0%
GGAAGAAGAAGATTTATCCA+TGG + chr3_2:83116362-83116381 Msa0431130:CDS 35.0%
!! TTAGCTTTGTGAGTTACCTT+GGG - chr3_2:83116573-83116592 None:intergenic 35.0%
!! ATTAGCTTTGTGAGTTACCT+TGG - chr3_2:83116574-83116593 None:intergenic 35.0%
TTCTTGTTGCAGAGAAAGAA+GGG - chr3_2:83116725-83116744 None:intergenic 35.0%
TTTCTCTGCAACAAGAACAA+AGG + chr3_2:83116728-83116747 Msa0431130:CDS 35.0%
! AAGCATTCAAGTTTCTTGGA+TGG + chr3_2:83116873-83116892 Msa0431130:CDS 35.0%
!! AAAAAGATGCAAGGTGTTGA+AGG + chr3_2:83117105-83117124 Msa0431130:three_prime_UTR 35.0%
ATGATAGGCAGAATTTGAAG+GGG - chr3_2:83117335-83117354 None:intergenic 35.0%
CTAGTGTTGTAGAGTGGTAA+AGG - chr3_2:83116080-83116099 None:intergenic 40.0%
CAATAGGAAAAGTGGGAAAG+TGG + chr3_2:83116293-83116312 Msa0431130:CDS 40.0%
!! AGTGGGAAAGTGGATTATGT+AGG + chr3_2:83116303-83116322 Msa0431130:CDS 40.0%
TGTAGGAAAAGGAGCAAGAA+TGG + chr3_2:83116320-83116339 Msa0431130:CDS 40.0%
!! AGAAGAAGCATCAGAAACCA+TGG - chr3_2:83116382-83116401 None:intergenic 40.0%
ATGGTTTCTGATGCTTCTTC+TGG + chr3_2:83116381-83116400 Msa0431130:CDS 40.0%
TCTACATGATAGTACTGTGG+AGG - chr3_2:83116408-83116427 None:intergenic 40.0%
TCCACAGTACTATCATGTAG+AGG + chr3_2:83116407-83116426 Msa0431130:CDS 40.0%
TCCTCTACATGATAGTACTG+TGG - chr3_2:83116411-83116430 None:intergenic 40.0%
GAACTAGCAGTATCATCAAG+AGG - chr3_2:83116534-83116553 None:intergenic 40.0%
CTTGGGATAGTTAAGAACAG+GGG - chr3_2:83116556-83116575 None:intergenic 40.0%
CCTTGGGATAGTTAAGAACA+GGG - chr3_2:83116557-83116576 None:intergenic 40.0%
ACCTTGGGATAGTTAAGAAC+AGG - chr3_2:83116558-83116577 None:intergenic 40.0%
TTTCTCAGAAAATGGAGCAG+TGG + chr3_2:83116680-83116699 Msa0431130:CDS 40.0%
TCTTGTTGCAGAGAAAGAAG+GGG - chr3_2:83116724-83116743 None:intergenic 40.0%
GTTCTTGTTGCAGAGAAAGA+AGG - chr3_2:83116726-83116745 None:intergenic 40.0%
CCAAGAAACTTGAATGCTTC+TGG - chr3_2:83116872-83116891 None:intergenic 40.0%
!! CCAGAAGCATTCAAGTTTCT+TGG + chr3_2:83116869-83116888 Msa0431130:CDS 40.0%
! CATTCAAGTTTCTTGGATGG+TGG + chr3_2:83116876-83116895 Msa0431130:CDS 40.0%
TGGTGGAAAACAAGCTTCAA+AGG + chr3_2:83116893-83116912 Msa0431130:CDS 40.0%
AAACAAGCTTCAAAGGAACC+AGG + chr3_2:83116900-83116919 Msa0431130:CDS 40.0%
!!! GTGGTTTTAATGGAGATGAG+AGG + chr3_2:83117024-83117043 Msa0431130:CDS 40.0%
TGCGTCAGAAGCTAAAAGTA+AGG + chr3_2:83117075-83117094 Msa0431130:three_prime_UTR 40.0%
GCGTCAGAAGCTAAAAGTAA+GGG + chr3_2:83117076-83117095 Msa0431130:three_prime_UTR 40.0%
GGGATGTGAAAAAAGATGCA+AGG + chr3_2:83117096-83117115 Msa0431130:three_prime_UTR 40.0%
TACGTTGTTCCAAACTGCAT+TGG - chr3_2:83117211-83117230 None:intergenic 40.0%
! GTTTGGAACAACGTAAGAAC+AGG + chr3_2:83117216-83117235 Msa0431130:three_prime_UTR 40.0%
CTAGTGTTGTAGAGTGGTAA+AGG - chr3_2:83116080-83116099 None:intergenic 40.0%
CAATAGGAAAAGTGGGAAAG+TGG + chr3_2:83116293-83116312 Msa0431130:CDS 40.0%
!! AGTGGGAAAGTGGATTATGT+AGG + chr3_2:83116303-83116322 Msa0431130:CDS 40.0%
TGTAGGAAAAGGAGCAAGAA+TGG + chr3_2:83116320-83116339 Msa0431130:CDS 40.0%
!! AGAAGAAGCATCAGAAACCA+TGG - chr3_2:83116382-83116401 None:intergenic 40.0%
ATGGTTTCTGATGCTTCTTC+TGG + chr3_2:83116381-83116400 Msa0431130:CDS 40.0%
TCTACATGATAGTACTGTGG+AGG - chr3_2:83116408-83116427 None:intergenic 40.0%
TCCACAGTACTATCATGTAG+AGG + chr3_2:83116407-83116426 Msa0431130:CDS 40.0%
TCCTCTACATGATAGTACTG+TGG - chr3_2:83116411-83116430 None:intergenic 40.0%
GAACTAGCAGTATCATCAAG+AGG - chr3_2:83116534-83116553 None:intergenic 40.0%
CTTGGGATAGTTAAGAACAG+GGG - chr3_2:83116556-83116575 None:intergenic 40.0%
CCTTGGGATAGTTAAGAACA+GGG - chr3_2:83116557-83116576 None:intergenic 40.0%
ACCTTGGGATAGTTAAGAAC+AGG - chr3_2:83116558-83116577 None:intergenic 40.0%
TTTCTCAGAAAATGGAGCAG+TGG + chr3_2:83116680-83116699 Msa0431130:CDS 40.0%
TCTTGTTGCAGAGAAAGAAG+GGG - chr3_2:83116724-83116743 None:intergenic 40.0%
GTTCTTGTTGCAGAGAAAGA+AGG - chr3_2:83116726-83116745 None:intergenic 40.0%
CCAAGAAACTTGAATGCTTC+TGG - chr3_2:83116872-83116891 None:intergenic 40.0%
!! CCAGAAGCATTCAAGTTTCT+TGG + chr3_2:83116869-83116888 Msa0431130:CDS 40.0%
! CATTCAAGTTTCTTGGATGG+TGG + chr3_2:83116876-83116895 Msa0431130:CDS 40.0%
TGGTGGAAAACAAGCTTCAA+AGG + chr3_2:83116893-83116912 Msa0431130:CDS 40.0%
AAACAAGCTTCAAAGGAACC+AGG + chr3_2:83116900-83116919 Msa0431130:CDS 40.0%
!!! GTGGTTTTAATGGAGATGAG+AGG + chr3_2:83117024-83117043 Msa0431130:CDS 40.0%
TGCGTCAGAAGCTAAAAGTA+AGG + chr3_2:83117075-83117094 Msa0431130:three_prime_UTR 40.0%
GCGTCAGAAGCTAAAAGTAA+GGG + chr3_2:83117076-83117095 Msa0431130:three_prime_UTR 40.0%
GGGATGTGAAAAAAGATGCA+AGG + chr3_2:83117096-83117115 Msa0431130:three_prime_UTR 40.0%
TACGTTGTTCCAAACTGCAT+TGG - chr3_2:83117211-83117230 None:intergenic 40.0%
! GTTTGGAACAACGTAAGAAC+AGG + chr3_2:83117216-83117235 Msa0431130:three_prime_UTR 40.0%
GGAAGAAGAAGAAGAAGAGG+AGG + chr3_2:83116341-83116360 Msa0431130:CDS 45.0%
CCCTGTTCTTAACTATCCCA+AGG + chr3_2:83116554-83116573 Msa0431130:CDS 45.0%
! TTCCAAACTGCATTGGCAGT+GGG - chr3_2:83117204-83117223 None:intergenic 45.0%
GGAAGAAGAAGAAGAAGAGG+AGG + chr3_2:83116341-83116360 Msa0431130:CDS 45.0%
CCCTGTTCTTAACTATCCCA+AGG + chr3_2:83116554-83116573 Msa0431130:CDS 45.0%
! TTCCAAACTGCATTGGCAGT+GGG - chr3_2:83117204-83117223 None:intergenic 45.0%
!!! TAATTATAACTAATTTTATT+TGG + chr3_2:83116611-83116630 Msa0431130:intron 5.0%
!!! TAATTATAACTAATTTTATT+TGG + chr3_2:83116611-83116630 Msa0431130:intron 5.0%
! GTTCCAAACTGCATTGGCAG+TGG - chr3_2:83117205-83117224 None:intergenic 50.0%
! GTTCCAAACTGCATTGGCAG+TGG - chr3_2:83117205-83117224 None:intergenic 50.0%
GTCCCACTGCCAATGCAGTT+TGG + chr3_2:83117199-83117218 Msa0431130:three_prime_UTR 55.0%
GTCCCACTGCCAATGCAGTT+TGG + chr3_2:83117199-83117218 Msa0431130:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 83116005 83117482 83116005 ID=Msa0431130;Name=Msa0431130
chr3_2 mRNA 83116005 83117482 83116005 ID=Msa0431130-mRNA-1;Parent=Msa0431130;Name=Msa0431130-mRNA-1;_AED=0.07;_eAED=0.07;_QI=196|1|1|1|1|1|4|430|183
chr3_2 exon 83116005 83116575 83116005 ID=Msa0431130-mRNA-1:exon:23144;Parent=Msa0431130-mRNA-1
chr3_2 exon 83116669 83116749 83116669 ID=Msa0431130-mRNA-1:exon:23145;Parent=Msa0431130-mRNA-1
chr3_2 exon 83116855 83116921 83116855 ID=Msa0431130-mRNA-1:exon:23146;Parent=Msa0431130-mRNA-1
chr3_2 exon 83117024 83117482 83117024 ID=Msa0431130-mRNA-1:exon:23147;Parent=Msa0431130-mRNA-1
chr3_2 five_prime_UTR 83116005 83116200 83116005 ID=Msa0431130-mRNA-1:five_prime_utr;Parent=Msa0431130-mRNA-1
chr3_2 CDS 83116201 83116575 83116201 ID=Msa0431130-mRNA-1:cds;Parent=Msa0431130-mRNA-1
chr3_2 CDS 83116669 83116749 83116669 ID=Msa0431130-mRNA-1:cds;Parent=Msa0431130-mRNA-1
chr3_2 CDS 83116855 83116921 83116855 ID=Msa0431130-mRNA-1:cds;Parent=Msa0431130-mRNA-1
chr3_2 CDS 83117024 83117052 83117024 ID=Msa0431130-mRNA-1:cds;Parent=Msa0431130-mRNA-1
chr3_2 three_prime_UTR 83117053 83117482 83117053 ID=Msa0431130-mRNA-1:three_prime_utr;Parent=Msa0431130-mRNA-1
Gene Sequence

>Msa0431130

ATGGATATGTCAAATTATTCAGAATACAATAGTGAAAGTGAATCTGGTTGGACTAATTACTTGAACCATTCATCTTTTTCTGAAGAACATTTCAATAGGAAAAGTGGGAAAGTGGATTATGTAGGAAAAGGAGCAAGAATGGAAGAAGAAGAAGAAGAGGAGGAAGAAGAAGATTTATCCATGGTTTCTGATGCTTCTTCTGGACCTCCACAGTACTATCATGTAGAGGAAAATTACCAACAACCTTATTGTGTAAATTGGAATTCTTCAACTTCCAAAGAATCCAAAAAGAAGTGTAAAGAATATGGTAGGAAAAGTCAACAGTCTTCACCTCTTGATGATACTGCTAGTTCCCCTGTTCTTAACTATCCCAAGAATAAAGTCAATTTCTCAGAAAATGGAGCAGTGGAGAATACACTTGATTTTTCCCCTTCTTTCTCTGCAACAAGAACAAAGAGAAAGTCTAAACTCCAGAAGCATTCAAGTTTCTTGGATGGTGGAAAACAAGCTTCAAAGGAACCAGGTGGTTTTAATGGAGATGAGAGGAAATGA
Protein sequence

>Msa0431130

MDMSNYSEYNSESESGWTNYLNHSSFSEEHFNRKSGKVDYVGKGARMEEEEEEEEEEDLSMVSDASSGPPQYYHVEENYQQPYCVNWNSSTSKESKKKCKEYGRKSQQSSPLDDTASSPVLNYPKNKVNFSENGAVENTLDFSPSFSATRTKRKSKLQKHSSFLDGGKQASKEPGGFNGDERK*