AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0381240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0381240 MtrunA17_Chr3g0134941 95.652 184 6 1 1 182 1 184 1.29e-126 353
Msa0381240 MtrunA17_Chr1g0152381 50.943 159 60 6 1 148 1 152 8.09e-32 112
Msa0381240 MtrunA17_Chr5g0393821 38.650 163 87 6 7 163 6 161 7.41e-15 68.9
Msa0381240 MtrunA17_Chr1g0207011 35.119 168 93 6 1 155 1 165 1.81e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0381240 AT4G33800.2 35.057 174 77 6 11 163 6 164 2.12e-12 62.8
Msa0381240 AT4G33800.1 35.057 174 77 6 11 163 6 164 2.12e-12 62.8
Msa0381240 AT1G58460.1 34.848 198 91 10 1 182 1 176 2.99e-11 59.7
Msa0381240 AT1G58460.2 36.000 175 78 9 1 163 1 153 7.92e-11 58.5

Find 58 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACCTTATTGTGTAAAT+TGG 0.134932 3_1:-83256655 Msa0381240:CDS
CCAGAAGCATTCAAGTTTCT+TGG 0.140446 3_1:-83256225 Msa0381240:CDS
TAGACAAGTGTTAGTTAAAA+TGG 0.155616 3_1:-83256909 Msa0381240:exon
ACTCTTATATATATAGATTT+TGG 0.190453 3_1:+83256973 None:intergenic
GAATTCCAATTTACACAATA+AGG 0.257445 3_1:+83256650 None:intergenic
CCAAGAAACTTGAATGCTTC+TGG 0.269813 3_1:+83256225 None:intergenic
AAAGTCAATTTCTCAGAAAA+TGG 0.272188 3_1:-83256422 Msa0381240:CDS
ATAAAGAAGGAACAGTTCTT+TGG 0.278886 3_1:-83255950 Msa0381240:three_prime_UTR
ATGGTTTCTGATGCTTCTTC+TGG 0.283239 3_1:-83256713 Msa0381240:CDS
CATGATGTCAACTAAGAAAA+TGG 0.287865 3_1:+83255841 None:intergenic
ACCTTGGGATAGTTAAGAAC+AGG 0.357740 3_1:+83256539 None:intergenic
TACAATAGTGAAAGTGAATC+TGG 0.379261 3_1:-83256866 Msa0381240:CDS
TTTCTGAAGAACATTTCAAT+AGG 0.386107 3_1:-83256814 Msa0381240:CDS
CAAGTGTATATTTAGTTTGT+CGG 0.388292 3_1:-83255681 Msa0381240:three_prime_UTR
AAAAGAAACTCTAGTTCTAT+AGG 0.414355 3_1:-83257047 Msa0381240:five_prime_UTR
AATTTACACAATAAGGTTGT+TGG 0.428691 3_1:+83256657 None:intergenic
TGTCGGATTGTCGCTCAGTT+TGG 0.441637 3_1:-83255664 Msa0381240:three_prime_UTR
GATGAAGTAAACATAATGAT+AGG 0.453876 3_1:+83255747 None:intergenic
GTCCCACTGCCAATGCAGTT+TGG 0.454243 3_1:-83255895 Msa0381240:three_prime_UTR
GTTCTTGTTGCAGAGAAAGA+AGG 0.454355 3_1:+83256371 None:intergenic
TGCGTCAGAAGCTAAAAGTA+AGG 0.464184 3_1:-83256019 Msa0381240:three_prime_UTR
GAACATTTCAATAGGAAAAG+TGG 0.473979 3_1:-83256806 Msa0381240:CDS
CAATAGGAAAAGTGGGAAAG+TGG 0.474045 3_1:-83256798 Msa0381240:CDS
CATTCAAGTTTCTTGGATGG+TGG 0.474914 3_1:-83256218 Msa0381240:CDS
CTAGTGTTGTAGAGTGGTAA+AGG 0.492301 3_1:+83257023 None:intergenic
GTTCCAAACTGCATTGGCAG+TGG 0.494977 3_1:+83255892 None:intergenic
AAAGTGGATTATGTAGGAAA+AGG 0.502120 3_1:-83256782 Msa0381240:CDS
TTCTTGTTGCAGAGAAAGAA+GGG 0.506519 3_1:+83256372 None:intergenic
GTTTGGAACAACGTAAGAAC+AGG 0.508021 3_1:-83255878 Msa0381240:three_prime_UTR
AACATTTCAATAGGAAAAGT+GGG 0.508806 3_1:-83256805 Msa0381240:CDS
TAATGATAGGCAGAATTTGA+AGG 0.517062 3_1:+83255760 None:intergenic
TACGTTGTTCCAAACTGCAT+TGG 0.529331 3_1:+83255886 None:intergenic
AACAATGGAAGAAGAAGAAG+AGG 0.533757 3_1:-83256756 Msa0381240:CDS
AATGATAGGCAGAATTTGAA+GGG 0.538940 3_1:+83255761 None:intergenic
TGGTGGAAAACAAGCTTCAA+AGG 0.539652 3_1:-83256201 Msa0381240:CDS
AAGCATTCAAGTTTCTTGGA+TGG 0.545383 3_1:-83256221 Msa0381240:CDS
CCCTGTTCTTAACTATCCCA+AGG 0.552465 3_1:-83256540 Msa0381240:intron
AGTGGGAAAGTGGATTATGT+AGG 0.561242 3_1:-83256788 Msa0381240:CDS
AGAAGTGTAAAGAATATGGT+AGG 0.566325 3_1:-83256604 Msa0381240:CDS
GGAAGAAGAAGATTTATCCA+TGG 0.566800 3_1:-83256732 Msa0381240:CDS
TGTAGGAAAAGGAGCAACAA+TGG 0.568877 3_1:-83256771 Msa0381240:CDS
AATGGAAGAAGAAGAAGAGG+AGG 0.569909 3_1:-83256753 Msa0381240:CDS
TTCCAAACTGCATTGGCAGT+GGG 0.575619 3_1:+83255893 None:intergenic
ATAGTGAAAGTGAATCTGGT+TGG 0.584733 3_1:-83256862 Msa0381240:CDS
AGAAGAAGCATCAGAAACCA+TGG 0.595770 3_1:+83256715 None:intergenic
CCTTGGGATAGTTAAGAACA+GGG 0.598514 3_1:+83256540 None:intergenic
GCGTCAGAAGCTAAAAGTAA+GGG 0.607870 3_1:-83256018 Msa0381240:three_prime_UTR
TTTCTCAGAAAATGGAGCAG+TGG 0.613686 3_1:-83256414 Msa0381240:CDS
TTTCTCTGCAACAAGAACAA+AGG 0.615674 3_1:-83256366 Msa0381240:intron
TCTTGTTGCAGAGAAAGAAG+GGG 0.652031 3_1:+83256373 None:intergenic
AAACAAGCTTCAAAGGAACC+AGG 0.657474 3_1:-83256194 Msa0381240:intron
TCCTCTACATGATAGTACTG+TGG 0.658500 3_1:+83256686 None:intergenic
GAACTAGCAGTATCATCAAG+AGG 0.659139 3_1:+83256563 None:intergenic
TGAAAACTAGTGTTGTAGAG+TGG 0.666228 3_1:+83257017 None:intergenic
ATGATAGGCAGAATTTGAAG+GGG 0.666686 3_1:+83255762 None:intergenic
TCCACAGTACTATCATGTAG+AGG 0.668253 3_1:-83256687 Msa0381240:CDS
TCTACATGATAGTACTGTGG+AGG 0.671420 3_1:+83256689 None:intergenic
CTTGGGATAGTTAAGAACAG+GGG 0.715448 3_1:+83256541 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATATAGATTTTGGTA+AGG + chr3_1:83255753-83255772 None:intergenic 15.0%
!!! ACTCTTATATATATAGATTT+TGG + chr3_1:83255758-83255777 None:intergenic 15.0%
!!! TTATAACTAATTTTATTTGG+AGG - chr3_1:83256248-83256267 Msa0381240:intron 15.0%
!! ATTAATTATGTATATATACC+TGG + chr3_1:83256555-83256574 None:intergenic 15.0%
!! AAAAATAGAGAAAACAAAAA+GGG + chr3_1:83257007-83257026 None:intergenic 15.0%
!! AAAAAATAGAGAAAACAAAA+AGG + chr3_1:83257008-83257027 None:intergenic 15.0%
!!! TTTGTTTTCTCTATTTTTTT+AGG - chr3_1:83257009-83257028 Msa0381240:five_prime_UTR 15.0%
!!! TATATATATAGATTTTGGTA+AGG + chr3_1:83255753-83255772 None:intergenic 15.0%
!!! ACTCTTATATATATAGATTT+TGG + chr3_1:83255758-83255777 None:intergenic 15.0%
!!! TTATAACTAATTTTATTTGG+AGG - chr3_1:83256248-83256267 Msa0381240:intron 15.0%
!! ATTAATTATGTATATATACC+TGG + chr3_1:83256555-83256574 None:intergenic 15.0%
!! AAAAATAGAGAAAACAAAAA+GGG + chr3_1:83257007-83257026 None:intergenic 15.0%
!! AAAAAATAGAGAAAACAAAA+AGG + chr3_1:83257008-83257027 None:intergenic 15.0%
!!! TTTGTTTTCTCTATTTTTTT+AGG - chr3_1:83257009-83257028 Msa0381240:five_prime_UTR 15.0%
!!! ATATTCTTTACACTTCTTTT+TGG + chr3_1:83256121-83256140 None:intergenic 20.0%
!!! AAAAAGAAGTGTAAAGAATA+TGG - chr3_1:83256120-83256139 Msa0381240:intron 20.0%
!! ATGCTTATTAGTTATTACAA+TGG - chr3_1:83256421-83256440 Msa0381240:CDS 20.0%
!! TTGTTTATGTCTTGATTATA+AGG - chr3_1:83256636-83256655 Msa0381240:CDS 20.0%
!! AAGTGATGTAAAAATAAAGA+AGG - chr3_1:83256765-83256784 Msa0381240:CDS 20.0%
!! AAAATAGAGAAAACAAAAAG+GGG + chr3_1:83257006-83257025 None:intergenic 20.0%
!!! CTAAATATACACTTGATTTT+TGG + chr3_1:83257042-83257061 None:intergenic 20.0%
!!! ATATTCTTTACACTTCTTTT+TGG + chr3_1:83256121-83256140 None:intergenic 20.0%
!!! AAAAAGAAGTGTAAAGAATA+TGG - chr3_1:83256120-83256139 Msa0381240:intron 20.0%
!! ATGCTTATTAGTTATTACAA+TGG - chr3_1:83256421-83256440 Msa0381240:CDS 20.0%
!! TTGTTTATGTCTTGATTATA+AGG - chr3_1:83256636-83256655 Msa0381240:CDS 20.0%
!! AAGTGATGTAAAAATAAAGA+AGG - chr3_1:83256765-83256784 Msa0381240:CDS 20.0%
!! AAAATAGAGAAAACAAAAAG+GGG + chr3_1:83257006-83257025 None:intergenic 20.0%
!!! CTAAATATACACTTGATTTT+TGG + chr3_1:83257042-83257061 None:intergenic 20.0%
!!! AAAAGAAACTCTAGTTCTAT+AGG - chr3_1:83255681-83255700 Msa0381240:three_prime_UTR 25.0%
! TAGACAAGTGTTAGTTAAAA+TGG - chr3_1:83255819-83255838 Msa0381240:three_prime_UTR 25.0%
!! TTTCTGAAGAACATTTCAAT+AGG - chr3_1:83255914-83255933 Msa0381240:three_prime_UTR 25.0%
! AACATTTCAATAGGAAAAGT+GGG - chr3_1:83255923-83255942 Msa0381240:three_prime_UTR 25.0%
! AATTTACACAATAAGGTTGT+TGG + chr3_1:83256074-83256093 None:intergenic 25.0%
! AACAACCTTATTGTGTAAAT+TGG - chr3_1:83256073-83256092 Msa0381240:CDS 25.0%
! GAATTCCAATTTACACAATA+AGG + chr3_1:83256081-83256100 None:intergenic 25.0%
! AAAGTCAATTTCTCAGAAAA+TGG - chr3_1:83256306-83256325 Msa0381240:intron 25.0%
! AACTAATAAGCATAATGTGT+TGG + chr3_1:83256414-83256433 None:intergenic 25.0%
!!! TGAATGTTTTAAGCTAATGA+AGG - chr3_1:83256612-83256631 Msa0381240:CDS 25.0%
! TTTATGTCTTGATTATAAGG+TGG - chr3_1:83256639-83256658 Msa0381240:CDS 25.0%
!!! TGATTATAAGGTGGTTTTAA+TGG - chr3_1:83256648-83256667 Msa0381240:CDS 25.0%
! GATGAAGTAAACATAATGAT+AGG + chr3_1:83256984-83257003 None:intergenic 25.0%
!!! CAAGTGTATATTTAGTTTGT+CGG - chr3_1:83257047-83257066 Msa0381240:five_prime_UTR 25.0%
!!! TCTTAATGTGTTTGTTTTCA+AGG + chr3_1:83255636-83255655 None:intergenic 25.0%
!!! AAAAGAAACTCTAGTTCTAT+AGG - chr3_1:83255681-83255700 Msa0381240:three_prime_UTR 25.0%
! TAGACAAGTGTTAGTTAAAA+TGG - chr3_1:83255819-83255838 Msa0381240:three_prime_UTR 25.0%
!! TTTCTGAAGAACATTTCAAT+AGG - chr3_1:83255914-83255933 Msa0381240:three_prime_UTR 25.0%
! AACATTTCAATAGGAAAAGT+GGG - chr3_1:83255923-83255942 Msa0381240:three_prime_UTR 25.0%
! AATTTACACAATAAGGTTGT+TGG + chr3_1:83256074-83256093 None:intergenic 25.0%
! AACAACCTTATTGTGTAAAT+TGG - chr3_1:83256073-83256092 Msa0381240:CDS 25.0%
! GAATTCCAATTTACACAATA+AGG + chr3_1:83256081-83256100 None:intergenic 25.0%
! AAAGTCAATTTCTCAGAAAA+TGG - chr3_1:83256306-83256325 Msa0381240:intron 25.0%
! AACTAATAAGCATAATGTGT+TGG + chr3_1:83256414-83256433 None:intergenic 25.0%
!!! TGAATGTTTTAAGCTAATGA+AGG - chr3_1:83256612-83256631 Msa0381240:CDS 25.0%
! TTTATGTCTTGATTATAAGG+TGG - chr3_1:83256639-83256658 Msa0381240:CDS 25.0%
!!! TGATTATAAGGTGGTTTTAA+TGG - chr3_1:83256648-83256667 Msa0381240:CDS 25.0%
! GATGAAGTAAACATAATGAT+AGG + chr3_1:83256984-83257003 None:intergenic 25.0%
!!! CAAGTGTATATTTAGTTTGT+CGG - chr3_1:83257047-83257066 Msa0381240:five_prime_UTR 25.0%
TACAATAGTGAAAGTGAATC+TGG - chr3_1:83255862-83255881 Msa0381240:three_prime_UTR 30.0%
GTTCTTCAGAAAAAGATGAA+TGG + chr3_1:83255906-83255925 None:intergenic 30.0%
GAACATTTCAATAGGAAAAG+TGG - chr3_1:83255922-83255941 Msa0381240:three_prime_UTR 30.0%
!! AAAGTGGATTATGTAGGAAA+AGG - chr3_1:83255946-83255965 Msa0381240:three_prime_UTR 30.0%
! ACACTTCTTTTTGGATTCTT+TGG + chr3_1:83256112-83256131 None:intergenic 30.0%
! AGAAGTGTAAAGAATATGGT+AGG - chr3_1:83256124-83256143 Msa0381240:intron 30.0%
TAAGCATAATGTGTTGGATT+TGG + chr3_1:83256408-83256427 None:intergenic 30.0%
ATAAAGAAGGAACAGTTCTT+TGG - chr3_1:83256778-83256797 Msa0381240:CDS 30.0%
CATGATGTCAACTAAGAAAA+TGG + chr3_1:83256890-83256909 None:intergenic 30.0%
! CATTTTCTTAGTTGACATCA+TGG - chr3_1:83256888-83256907 Msa0381240:CDS 30.0%
! CATGTCTTTTTGAATCTCTT+AGG - chr3_1:83256915-83256934 Msa0381240:five_prime_UTR 30.0%
AATGATAGGCAGAATTTGAA+GGG + chr3_1:83256970-83256989 None:intergenic 30.0%
TAATGATAGGCAGAATTTGA+AGG + chr3_1:83256971-83256990 None:intergenic 30.0%
TACAATAGTGAAAGTGAATC+TGG - chr3_1:83255862-83255881 Msa0381240:three_prime_UTR 30.0%
GTTCTTCAGAAAAAGATGAA+TGG + chr3_1:83255906-83255925 None:intergenic 30.0%
GAACATTTCAATAGGAAAAG+TGG - chr3_1:83255922-83255941 Msa0381240:three_prime_UTR 30.0%
!! AAAGTGGATTATGTAGGAAA+AGG - chr3_1:83255946-83255965 Msa0381240:three_prime_UTR 30.0%
! ACACTTCTTTTTGGATTCTT+TGG + chr3_1:83256112-83256131 None:intergenic 30.0%
! AGAAGTGTAAAGAATATGGT+AGG - chr3_1:83256124-83256143 Msa0381240:intron 30.0%
TAAGCATAATGTGTTGGATT+TGG + chr3_1:83256408-83256427 None:intergenic 30.0%
ATAAAGAAGGAACAGTTCTT+TGG - chr3_1:83256778-83256797 Msa0381240:CDS 30.0%
CATGATGTCAACTAAGAAAA+TGG + chr3_1:83256890-83256909 None:intergenic 30.0%
! CATTTTCTTAGTTGACATCA+TGG - chr3_1:83256888-83256907 Msa0381240:CDS 30.0%
! CATGTCTTTTTGAATCTCTT+AGG - chr3_1:83256915-83256934 Msa0381240:five_prime_UTR 30.0%
AATGATAGGCAGAATTTGAA+GGG + chr3_1:83256970-83256989 None:intergenic 30.0%
TAATGATAGGCAGAATTTGA+AGG + chr3_1:83256971-83256990 None:intergenic 30.0%
TGAAAACTAGTGTTGTAGAG+TGG + chr3_1:83255714-83255733 None:intergenic 35.0%
ATAGTGAAAGTGAATCTGGT+TGG - chr3_1:83255866-83255885 Msa0381240:three_prime_UTR 35.0%
AACAATGGAAGAAGAAGAAG+AGG - chr3_1:83255972-83255991 Msa0381240:three_prime_UTR 35.0%
GGAAGAAGAAGATTTATCCA+TGG - chr3_1:83255996-83256015 Msa0381240:three_prime_UTR 35.0%
!! TTAGCTTTGTGAGTTACCTT+GGG + chr3_1:83256207-83256226 None:intergenic 35.0%
!! ATTAGCTTTGTGAGTTACCT+TGG + chr3_1:83256208-83256227 None:intergenic 35.0%
TTCTTGTTGCAGAGAAAGAA+GGG + chr3_1:83256359-83256378 None:intergenic 35.0%
TTTCTCTGCAACAAGAACAA+AGG - chr3_1:83256362-83256381 Msa0381240:intron 35.0%
! AAGCATTCAAGTTTCTTGGA+TGG - chr3_1:83256507-83256526 Msa0381240:intron 35.0%
!! AAAAAGATGCAAGGTGTTGA+AGG - chr3_1:83256739-83256758 Msa0381240:CDS 35.0%
ATGATAGGCAGAATTTGAAG+GGG + chr3_1:83256969-83256988 None:intergenic 35.0%
TGAAAACTAGTGTTGTAGAG+TGG + chr3_1:83255714-83255733 None:intergenic 35.0%
ATAGTGAAAGTGAATCTGGT+TGG - chr3_1:83255866-83255885 Msa0381240:three_prime_UTR 35.0%
AACAATGGAAGAAGAAGAAG+AGG - chr3_1:83255972-83255991 Msa0381240:three_prime_UTR 35.0%
GGAAGAAGAAGATTTATCCA+TGG - chr3_1:83255996-83256015 Msa0381240:three_prime_UTR 35.0%
!! TTAGCTTTGTGAGTTACCTT+GGG + chr3_1:83256207-83256226 None:intergenic 35.0%
!! ATTAGCTTTGTGAGTTACCT+TGG + chr3_1:83256208-83256227 None:intergenic 35.0%
TTCTTGTTGCAGAGAAAGAA+GGG + chr3_1:83256359-83256378 None:intergenic 35.0%
TTTCTCTGCAACAAGAACAA+AGG - chr3_1:83256362-83256381 Msa0381240:intron 35.0%
! AAGCATTCAAGTTTCTTGGA+TGG - chr3_1:83256507-83256526 Msa0381240:intron 35.0%
!! AAAAAGATGCAAGGTGTTGA+AGG - chr3_1:83256739-83256758 Msa0381240:CDS 35.0%
ATGATAGGCAGAATTTGAAG+GGG + chr3_1:83256969-83256988 None:intergenic 35.0%
CTAGTGTTGTAGAGTGGTAA+AGG + chr3_1:83255708-83255727 None:intergenic 40.0%
CAATAGGAAAAGTGGGAAAG+TGG - chr3_1:83255930-83255949 Msa0381240:three_prime_UTR 40.0%
!! AGTGGGAAAGTGGATTATGT+AGG - chr3_1:83255940-83255959 Msa0381240:three_prime_UTR 40.0%
TGTAGGAAAAGGAGCAACAA+TGG - chr3_1:83255957-83255976 Msa0381240:three_prime_UTR 40.0%
AATGGAAGAAGAAGAAGAGG+AGG - chr3_1:83255975-83255994 Msa0381240:three_prime_UTR 40.0%
!! AGAAGAAGCATCAGAAACCA+TGG + chr3_1:83256016-83256035 None:intergenic 40.0%
ATGGTTTCTGATGCTTCTTC+TGG - chr3_1:83256015-83256034 Msa0381240:three_prime_UTR 40.0%
TCTACATGATAGTACTGTGG+AGG + chr3_1:83256042-83256061 None:intergenic 40.0%
TCCACAGTACTATCATGTAG+AGG - chr3_1:83256041-83256060 Msa0381240:three_prime_UTR 40.0%
TCCTCTACATGATAGTACTG+TGG + chr3_1:83256045-83256064 None:intergenic 40.0%
GAACTAGCAGTATCATCAAG+AGG + chr3_1:83256168-83256187 None:intergenic 40.0%
CTTGGGATAGTTAAGAACAG+GGG + chr3_1:83256190-83256209 None:intergenic 40.0%
CCTTGGGATAGTTAAGAACA+GGG + chr3_1:83256191-83256210 None:intergenic 40.0%
ACCTTGGGATAGTTAAGAAC+AGG + chr3_1:83256192-83256211 None:intergenic 40.0%
TTTCTCAGAAAATGGAGCAG+TGG - chr3_1:83256314-83256333 Msa0381240:intron 40.0%
TCTTGTTGCAGAGAAAGAAG+GGG + chr3_1:83256358-83256377 None:intergenic 40.0%
GTTCTTGTTGCAGAGAAAGA+AGG + chr3_1:83256360-83256379 None:intergenic 40.0%
CCAAGAAACTTGAATGCTTC+TGG + chr3_1:83256506-83256525 None:intergenic 40.0%
!! CCAGAAGCATTCAAGTTTCT+TGG - chr3_1:83256503-83256522 Msa0381240:intron 40.0%
! CATTCAAGTTTCTTGGATGG+TGG - chr3_1:83256510-83256529 Msa0381240:intron 40.0%
TGGTGGAAAACAAGCTTCAA+AGG - chr3_1:83256527-83256546 Msa0381240:intron 40.0%
AAACAAGCTTCAAAGGAACC+AGG - chr3_1:83256534-83256553 Msa0381240:intron 40.0%
!!! GTGGTTTTAATGGAGATGAG+AGG - chr3_1:83256658-83256677 Msa0381240:CDS 40.0%
TGCGTCAGAAGCTAAAAGTA+AGG - chr3_1:83256709-83256728 Msa0381240:CDS 40.0%
GCGTCAGAAGCTAAAAGTAA+GGG - chr3_1:83256710-83256729 Msa0381240:CDS 40.0%
GGGATGTGAAAAAAGATGCA+AGG - chr3_1:83256730-83256749 Msa0381240:CDS 40.0%
TACGTTGTTCCAAACTGCAT+TGG + chr3_1:83256845-83256864 None:intergenic 40.0%
! GTTTGGAACAACGTAAGAAC+AGG - chr3_1:83256850-83256869 Msa0381240:CDS 40.0%
CTAGTGTTGTAGAGTGGTAA+AGG + chr3_1:83255708-83255727 None:intergenic 40.0%
CAATAGGAAAAGTGGGAAAG+TGG - chr3_1:83255930-83255949 Msa0381240:three_prime_UTR 40.0%
!! AGTGGGAAAGTGGATTATGT+AGG - chr3_1:83255940-83255959 Msa0381240:three_prime_UTR 40.0%
TGTAGGAAAAGGAGCAACAA+TGG - chr3_1:83255957-83255976 Msa0381240:three_prime_UTR 40.0%
AATGGAAGAAGAAGAAGAGG+AGG - chr3_1:83255975-83255994 Msa0381240:three_prime_UTR 40.0%
!! AGAAGAAGCATCAGAAACCA+TGG + chr3_1:83256016-83256035 None:intergenic 40.0%
ATGGTTTCTGATGCTTCTTC+TGG - chr3_1:83256015-83256034 Msa0381240:three_prime_UTR 40.0%
TCTACATGATAGTACTGTGG+AGG + chr3_1:83256042-83256061 None:intergenic 40.0%
TCCACAGTACTATCATGTAG+AGG - chr3_1:83256041-83256060 Msa0381240:three_prime_UTR 40.0%
TCCTCTACATGATAGTACTG+TGG + chr3_1:83256045-83256064 None:intergenic 40.0%
GAACTAGCAGTATCATCAAG+AGG + chr3_1:83256168-83256187 None:intergenic 40.0%
CTTGGGATAGTTAAGAACAG+GGG + chr3_1:83256190-83256209 None:intergenic 40.0%
CCTTGGGATAGTTAAGAACA+GGG + chr3_1:83256191-83256210 None:intergenic 40.0%
ACCTTGGGATAGTTAAGAAC+AGG + chr3_1:83256192-83256211 None:intergenic 40.0%
TTTCTCAGAAAATGGAGCAG+TGG - chr3_1:83256314-83256333 Msa0381240:intron 40.0%
TCTTGTTGCAGAGAAAGAAG+GGG + chr3_1:83256358-83256377 None:intergenic 40.0%
GTTCTTGTTGCAGAGAAAGA+AGG + chr3_1:83256360-83256379 None:intergenic 40.0%
CCAAGAAACTTGAATGCTTC+TGG + chr3_1:83256506-83256525 None:intergenic 40.0%
!! CCAGAAGCATTCAAGTTTCT+TGG - chr3_1:83256503-83256522 Msa0381240:intron 40.0%
! CATTCAAGTTTCTTGGATGG+TGG - chr3_1:83256510-83256529 Msa0381240:intron 40.0%
TGGTGGAAAACAAGCTTCAA+AGG - chr3_1:83256527-83256546 Msa0381240:intron 40.0%
AAACAAGCTTCAAAGGAACC+AGG - chr3_1:83256534-83256553 Msa0381240:intron 40.0%
!!! GTGGTTTTAATGGAGATGAG+AGG - chr3_1:83256658-83256677 Msa0381240:CDS 40.0%
TGCGTCAGAAGCTAAAAGTA+AGG - chr3_1:83256709-83256728 Msa0381240:CDS 40.0%
GCGTCAGAAGCTAAAAGTAA+GGG - chr3_1:83256710-83256729 Msa0381240:CDS 40.0%
GGGATGTGAAAAAAGATGCA+AGG - chr3_1:83256730-83256749 Msa0381240:CDS 40.0%
TACGTTGTTCCAAACTGCAT+TGG + chr3_1:83256845-83256864 None:intergenic 40.0%
! GTTTGGAACAACGTAAGAAC+AGG - chr3_1:83256850-83256869 Msa0381240:CDS 40.0%
CCCTGTTCTTAACTATCCCA+AGG - chr3_1:83256188-83256207 Msa0381240:intron 45.0%
! TTCCAAACTGCATTGGCAGT+GGG + chr3_1:83256838-83256857 None:intergenic 45.0%
CCCTGTTCTTAACTATCCCA+AGG - chr3_1:83256188-83256207 Msa0381240:intron 45.0%
! TTCCAAACTGCATTGGCAGT+GGG + chr3_1:83256838-83256857 None:intergenic 45.0%
!!! TAATTATAACTAATTTTATT+TGG - chr3_1:83256245-83256264 Msa0381240:intron 5.0%
!!! TAATTATAACTAATTTTATT+TGG - chr3_1:83256245-83256264 Msa0381240:intron 5.0%
! GTTCCAAACTGCATTGGCAG+TGG + chr3_1:83256839-83256858 None:intergenic 50.0%
TGTCGGATTGTCGCTCAGTT+TGG - chr3_1:83257064-83257083 Msa0381240:five_prime_UTR 50.0%
! GTTCCAAACTGCATTGGCAG+TGG + chr3_1:83256839-83256858 None:intergenic 50.0%
TGTCGGATTGTCGCTCAGTT+TGG - chr3_1:83257064-83257083 Msa0381240:five_prime_UTR 50.0%
GTCCCACTGCCAATGCAGTT+TGG - chr3_1:83256833-83256852 Msa0381240:CDS 55.0%
GTCCCACTGCCAATGCAGTT+TGG - chr3_1:83256833-83256852 Msa0381240:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 83255634 83257116 83255634 ID=Msa0381240;Name=Msa0381240
chr3_1 mRNA 83255634 83257116 83255634 ID=Msa0381240-mRNA-1;Parent=Msa0381240;Name=Msa0381240-mRNA-1;_AED=0.07;_eAED=0.07;_QI=204|1|1|1|1|1|4|430|182
chr3_1 exon 83255634 83256092 83255634 ID=Msa0381240-mRNA-1:exon:21350;Parent=Msa0381240-mRNA-1
chr3_1 exon 83256195 83256261 83256195 ID=Msa0381240-mRNA-1:exon:21349;Parent=Msa0381240-mRNA-1
chr3_1 exon 83256367 83256447 83256367 ID=Msa0381240-mRNA-1:exon:21348;Parent=Msa0381240-mRNA-1
chr3_1 exon 83256541 83257116 83256541 ID=Msa0381240-mRNA-1:exon:21347;Parent=Msa0381240-mRNA-1
chr3_1 five_prime_UTR 83256913 83257116 83256913 ID=Msa0381240-mRNA-1:five_prime_utr;Parent=Msa0381240-mRNA-1
chr3_1 CDS 83256541 83256912 83256541 ID=Msa0381240-mRNA-1:cds;Parent=Msa0381240-mRNA-1
chr3_1 CDS 83256367 83256447 83256367 ID=Msa0381240-mRNA-1:cds;Parent=Msa0381240-mRNA-1
chr3_1 CDS 83256195 83256261 83256195 ID=Msa0381240-mRNA-1:cds;Parent=Msa0381240-mRNA-1
chr3_1 CDS 83256064 83256092 83256064 ID=Msa0381240-mRNA-1:cds;Parent=Msa0381240-mRNA-1
chr3_1 three_prime_UTR 83255634 83256063 83255634 ID=Msa0381240-mRNA-1:three_prime_utr;Parent=Msa0381240-mRNA-1
Gene Sequence

>Msa0381240

ATGGATATGTCAAATTATTCAGAATACAATAGTGAAAGTGAATCTGGTTGGACTAATTACTTGAACCATTCATCTTTTTCTGAAGAACATTTCAATAGGAAAAGTGGGAAAGTGGATTATGTAGGAAAAGGAGCAACAATGGAAGAAGAAGAAGAGGAGGAAGAAGAAGATTTATCCATGGTTTCTGATGCTTCTTCTGGACCTCCACAGTACTATCATGTAGAGGAAAATTACCAACAACCTTATTGTGTAAATTGGAATTCTTCAACTTCCAAAGAATCCAAAAAGAAGTGTAAAGAATATGGTAGGAAAAGTCAACAGTCTTCACCTCTTGATGATACTGCTAGTTCCCCTGTTCTTAACTATCCCAAGAATAAAGTCAATTTCTCAGAAAATGGAGCAGTGGAGAATACACTTGATTTTTCCCCTTCTTTCTCTGCAACAAGAACAAAGAGAAAGTCTAAACTCCAGAAGCATTCAAGTTTCTTGGATGGTGGAAAACAAGCTTCAAAGGAACCAGGTGGTTTTAATGGAGATGAGAGGAAATGA
Protein sequence

>Msa0381240

MDMSNYSEYNSESESGWTNYLNHSSFSEEHFNRKSGKVDYVGKGATMEEEEEEEEEDLSMVSDASSGPPQYYHVEENYQQPYCVNWNSSTSKESKKKCKEYGRKSQQSSPLDDTASSPVLNYPKNKVNFSENGAVENTLDFSPSFSATRTKRKSKLQKHSSFLDGGKQASKEPGGFNGDERK*