AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0431150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431150 MtrunA17_Chr3g0134941 95.652 184 6 1 1 182 1 184 1.29e-126 353
Msa0431150 MtrunA17_Chr1g0152381 50.943 159 60 6 1 148 1 152 8.09e-32 112
Msa0431150 MtrunA17_Chr5g0393821 38.650 163 87 6 7 163 6 161 7.41e-15 68.9
Msa0431150 MtrunA17_Chr1g0207011 35.119 168 93 6 1 155 1 165 1.81e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431150 AT4G33800.2 35.057 174 77 6 11 163 6 164 2.12e-12 62.8
Msa0431150 AT4G33800.1 35.057 174 77 6 11 163 6 164 2.12e-12 62.8
Msa0431150 AT1G58460.1 34.848 198 91 10 1 182 1 176 2.99e-11 59.7
Msa0431150 AT1G58460.2 36.000 175 78 9 1 163 1 153 7.92e-11 58.5

Find 58 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACCTTATTGTGTAAAT+TGG 0.134932 3_2:-83142241 Msa0431150:CDS
CCAGAAGCATTCAAGTTTCT+TGG 0.140446 3_2:-83141811 Msa0431150:CDS
TAGACAAGTGTTAGTTAAAA+TGG 0.155616 3_2:-83142495 Msa0431150:exon
ACTCTTATATATATAGATTT+TGG 0.190453 3_2:+83142559 None:intergenic
GAATTCCAATTTACACAATA+AGG 0.257445 3_2:+83142236 None:intergenic
CCAAGAAACTTGAATGCTTC+TGG 0.269813 3_2:+83141811 None:intergenic
AAAGTCAATTTCTCAGAAAA+TGG 0.272188 3_2:-83142008 Msa0431150:CDS
ATAAAGAAGGAACAGTTCTT+TGG 0.278886 3_2:-83141536 Msa0431150:three_prime_UTR
ATGGTTTCTGATGCTTCTTC+TGG 0.283239 3_2:-83142299 Msa0431150:CDS
CATGATGTCAACTAAGAAAA+TGG 0.287865 3_2:+83141427 None:intergenic
ACCTTGGGATAGTTAAGAAC+AGG 0.357740 3_2:+83142125 None:intergenic
TACAATAGTGAAAGTGAATC+TGG 0.379261 3_2:-83142452 Msa0431150:CDS
TTTCTGAAGAACATTTCAAT+AGG 0.386107 3_2:-83142400 Msa0431150:CDS
CAAGTGTATATTTAGTTTGT+CGG 0.388292 3_2:-83141267 Msa0431150:three_prime_UTR
AAAAGAAACTCTAGTTCTAT+AGG 0.414355 3_2:-83142633 Msa0431150:five_prime_UTR
AATTTACACAATAAGGTTGT+TGG 0.428691 3_2:+83142243 None:intergenic
TGTCGGATTGTCGCTCAGTT+TGG 0.441637 3_2:-83141250 Msa0431150:three_prime_UTR
GATGAAGTAAACATAATGAT+AGG 0.453876 3_2:+83141333 None:intergenic
GTCCCACTGCCAATGCAGTT+TGG 0.454243 3_2:-83141481 Msa0431150:three_prime_UTR
GTTCTTGTTGCAGAGAAAGA+AGG 0.454355 3_2:+83141957 None:intergenic
TGCGTCAGAAGCTAAAAGTA+AGG 0.464184 3_2:-83141605 Msa0431150:three_prime_UTR
GAACATTTCAATAGGAAAAG+TGG 0.473979 3_2:-83142392 Msa0431150:CDS
CAATAGGAAAAGTGGGAAAG+TGG 0.474045 3_2:-83142384 Msa0431150:CDS
CATTCAAGTTTCTTGGATGG+TGG 0.474914 3_2:-83141804 Msa0431150:CDS
CTAGTGTTGTAGAGTGGTAA+AGG 0.492301 3_2:+83142609 None:intergenic
GTTCCAAACTGCATTGGCAG+TGG 0.494977 3_2:+83141478 None:intergenic
AAAGTGGATTATGTAGGAAA+AGG 0.502120 3_2:-83142368 Msa0431150:CDS
TTCTTGTTGCAGAGAAAGAA+GGG 0.506519 3_2:+83141958 None:intergenic
GTTTGGAACAACGTAAGAAC+AGG 0.508021 3_2:-83141464 Msa0431150:three_prime_UTR
AACATTTCAATAGGAAAAGT+GGG 0.508806 3_2:-83142391 Msa0431150:CDS
TAATGATAGGCAGAATTTGA+AGG 0.517062 3_2:+83141346 None:intergenic
TACGTTGTTCCAAACTGCAT+TGG 0.529331 3_2:+83141472 None:intergenic
AACAATGGAAGAAGAAGAAG+AGG 0.533757 3_2:-83142342 Msa0431150:CDS
AATGATAGGCAGAATTTGAA+GGG 0.538940 3_2:+83141347 None:intergenic
TGGTGGAAAACAAGCTTCAA+AGG 0.539652 3_2:-83141787 Msa0431150:CDS
AAGCATTCAAGTTTCTTGGA+TGG 0.545383 3_2:-83141807 Msa0431150:CDS
CCCTGTTCTTAACTATCCCA+AGG 0.552465 3_2:-83142126 Msa0431150:intron
AGTGGGAAAGTGGATTATGT+AGG 0.561242 3_2:-83142374 Msa0431150:CDS
AGAAGTGTAAAGAATATGGT+AGG 0.566325 3_2:-83142190 Msa0431150:CDS
GGAAGAAGAAGATTTATCCA+TGG 0.566800 3_2:-83142318 Msa0431150:CDS
TGTAGGAAAAGGAGCAACAA+TGG 0.568877 3_2:-83142357 Msa0431150:CDS
AATGGAAGAAGAAGAAGAGG+AGG 0.569909 3_2:-83142339 Msa0431150:CDS
TTCCAAACTGCATTGGCAGT+GGG 0.575619 3_2:+83141479 None:intergenic
ATAGTGAAAGTGAATCTGGT+TGG 0.584733 3_2:-83142448 Msa0431150:CDS
AGAAGAAGCATCAGAAACCA+TGG 0.595770 3_2:+83142301 None:intergenic
CCTTGGGATAGTTAAGAACA+GGG 0.598514 3_2:+83142126 None:intergenic
GCGTCAGAAGCTAAAAGTAA+GGG 0.607870 3_2:-83141604 Msa0431150:three_prime_UTR
TTTCTCAGAAAATGGAGCAG+TGG 0.613686 3_2:-83142000 Msa0431150:CDS
TTTCTCTGCAACAAGAACAA+AGG 0.615674 3_2:-83141952 Msa0431150:intron
TCTTGTTGCAGAGAAAGAAG+GGG 0.652031 3_2:+83141959 None:intergenic
AAACAAGCTTCAAAGGAACC+AGG 0.657474 3_2:-83141780 Msa0431150:intron
TCCTCTACATGATAGTACTG+TGG 0.658500 3_2:+83142272 None:intergenic
GAACTAGCAGTATCATCAAG+AGG 0.659139 3_2:+83142149 None:intergenic
TGAAAACTAGTGTTGTAGAG+TGG 0.666228 3_2:+83142603 None:intergenic
ATGATAGGCAGAATTTGAAG+GGG 0.666686 3_2:+83141348 None:intergenic
TCCACAGTACTATCATGTAG+AGG 0.668253 3_2:-83142273 Msa0431150:CDS
TCTACATGATAGTACTGTGG+AGG 0.671420 3_2:+83142275 None:intergenic
CTTGGGATAGTTAAGAACAG+GGG 0.715448 3_2:+83142127 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATATAGATTTTGGTA+AGG + chr3_2:83141340-83141359 None:intergenic 15.0%
!!! ACTCTTATATATATAGATTT+TGG + chr3_2:83141345-83141364 None:intergenic 15.0%
!!! TTATAACTAATTTTATTTGG+AGG - chr3_2:83141835-83141854 Msa0431150:intron 15.0%
!! ATTAATTATGTATATATACC+TGG + chr3_2:83142142-83142161 None:intergenic 15.0%
!! AAAAATAGAGAAAACAAAAA+GGG + chr3_2:83142594-83142613 None:intergenic 15.0%
!! AAAAAATAGAGAAAACAAAA+AGG + chr3_2:83142595-83142614 None:intergenic 15.0%
!!! TTTGTTTTCTCTATTTTTTT+AGG - chr3_2:83142596-83142615 Msa0431150:five_prime_UTR 15.0%
!!! TATATATATAGATTTTGGTA+AGG + chr3_2:83141340-83141359 None:intergenic 15.0%
!!! ACTCTTATATATATAGATTT+TGG + chr3_2:83141345-83141364 None:intergenic 15.0%
!!! TTATAACTAATTTTATTTGG+AGG - chr3_2:83141835-83141854 Msa0431150:intron 15.0%
!! ATTAATTATGTATATATACC+TGG + chr3_2:83142142-83142161 None:intergenic 15.0%
!! AAAAATAGAGAAAACAAAAA+GGG + chr3_2:83142594-83142613 None:intergenic 15.0%
!! AAAAAATAGAGAAAACAAAA+AGG + chr3_2:83142595-83142614 None:intergenic 15.0%
!!! TTTGTTTTCTCTATTTTTTT+AGG - chr3_2:83142596-83142615 Msa0431150:five_prime_UTR 15.0%
!!! ATATTCTTTACACTTCTTTT+TGG + chr3_2:83141708-83141727 None:intergenic 20.0%
!!! AAAAAGAAGTGTAAAGAATA+TGG - chr3_2:83141707-83141726 Msa0431150:intron 20.0%
!! ATGCTTATTAGTTATTACAA+TGG - chr3_2:83142008-83142027 Msa0431150:CDS 20.0%
!! TTGTTTATGTCTTGATTATA+AGG - chr3_2:83142223-83142242 Msa0431150:CDS 20.0%
!! AAGTGATGTAAAAATAAAGA+AGG - chr3_2:83142352-83142371 Msa0431150:CDS 20.0%
!! AAAATAGAGAAAACAAAAAG+GGG + chr3_2:83142593-83142612 None:intergenic 20.0%
!!! CTAAATATACACTTGATTTT+TGG + chr3_2:83142629-83142648 None:intergenic 20.0%
!!! ATATTCTTTACACTTCTTTT+TGG + chr3_2:83141708-83141727 None:intergenic 20.0%
!!! AAAAAGAAGTGTAAAGAATA+TGG - chr3_2:83141707-83141726 Msa0431150:intron 20.0%
!! ATGCTTATTAGTTATTACAA+TGG - chr3_2:83142008-83142027 Msa0431150:CDS 20.0%
!! TTGTTTATGTCTTGATTATA+AGG - chr3_2:83142223-83142242 Msa0431150:CDS 20.0%
!! AAGTGATGTAAAAATAAAGA+AGG - chr3_2:83142352-83142371 Msa0431150:CDS 20.0%
!! AAAATAGAGAAAACAAAAAG+GGG + chr3_2:83142593-83142612 None:intergenic 20.0%
!!! CTAAATATACACTTGATTTT+TGG + chr3_2:83142629-83142648 None:intergenic 20.0%
!!! AAAAGAAACTCTAGTTCTAT+AGG - chr3_2:83141268-83141287 Msa0431150:three_prime_UTR 25.0%
! TAGACAAGTGTTAGTTAAAA+TGG - chr3_2:83141406-83141425 Msa0431150:three_prime_UTR 25.0%
!! TTTCTGAAGAACATTTCAAT+AGG - chr3_2:83141501-83141520 Msa0431150:three_prime_UTR 25.0%
! AACATTTCAATAGGAAAAGT+GGG - chr3_2:83141510-83141529 Msa0431150:three_prime_UTR 25.0%
! AATTTACACAATAAGGTTGT+TGG + chr3_2:83141661-83141680 None:intergenic 25.0%
! AACAACCTTATTGTGTAAAT+TGG - chr3_2:83141660-83141679 Msa0431150:intron 25.0%
! GAATTCCAATTTACACAATA+AGG + chr3_2:83141668-83141687 None:intergenic 25.0%
! AAAGTCAATTTCTCAGAAAA+TGG - chr3_2:83141893-83141912 Msa0431150:intron 25.0%
! AACTAATAAGCATAATGTGT+TGG + chr3_2:83142001-83142020 None:intergenic 25.0%
!!! TGAATGTTTTAAGCTAATGA+AGG - chr3_2:83142199-83142218 Msa0431150:CDS 25.0%
! TTTATGTCTTGATTATAAGG+TGG - chr3_2:83142226-83142245 Msa0431150:CDS 25.0%
!!! TGATTATAAGGTGGTTTTAA+TGG - chr3_2:83142235-83142254 Msa0431150:CDS 25.0%
! GATGAAGTAAACATAATGAT+AGG + chr3_2:83142571-83142590 None:intergenic 25.0%
!!! CAAGTGTATATTTAGTTTGT+CGG - chr3_2:83142634-83142653 Msa0431150:five_prime_UTR 25.0%
!!! TCTTAATGTGTTTGTTTTCA+AGG + chr3_2:83141222-83141241 None:intergenic 25.0%
!!! AAAAGAAACTCTAGTTCTAT+AGG - chr3_2:83141268-83141287 Msa0431150:three_prime_UTR 25.0%
! TAGACAAGTGTTAGTTAAAA+TGG - chr3_2:83141406-83141425 Msa0431150:three_prime_UTR 25.0%
!! TTTCTGAAGAACATTTCAAT+AGG - chr3_2:83141501-83141520 Msa0431150:three_prime_UTR 25.0%
! AACATTTCAATAGGAAAAGT+GGG - chr3_2:83141510-83141529 Msa0431150:three_prime_UTR 25.0%
! AATTTACACAATAAGGTTGT+TGG + chr3_2:83141661-83141680 None:intergenic 25.0%
! AACAACCTTATTGTGTAAAT+TGG - chr3_2:83141660-83141679 Msa0431150:intron 25.0%
! GAATTCCAATTTACACAATA+AGG + chr3_2:83141668-83141687 None:intergenic 25.0%
! AAAGTCAATTTCTCAGAAAA+TGG - chr3_2:83141893-83141912 Msa0431150:intron 25.0%
! AACTAATAAGCATAATGTGT+TGG + chr3_2:83142001-83142020 None:intergenic 25.0%
!!! TGAATGTTTTAAGCTAATGA+AGG - chr3_2:83142199-83142218 Msa0431150:CDS 25.0%
! TTTATGTCTTGATTATAAGG+TGG - chr3_2:83142226-83142245 Msa0431150:CDS 25.0%
!!! TGATTATAAGGTGGTTTTAA+TGG - chr3_2:83142235-83142254 Msa0431150:CDS 25.0%
! GATGAAGTAAACATAATGAT+AGG + chr3_2:83142571-83142590 None:intergenic 25.0%
!!! CAAGTGTATATTTAGTTTGT+CGG - chr3_2:83142634-83142653 Msa0431150:five_prime_UTR 25.0%
TACAATAGTGAAAGTGAATC+TGG - chr3_2:83141449-83141468 Msa0431150:three_prime_UTR 30.0%
GTTCTTCAGAAAAAGATGAA+TGG + chr3_2:83141493-83141512 None:intergenic 30.0%
GAACATTTCAATAGGAAAAG+TGG - chr3_2:83141509-83141528 Msa0431150:three_prime_UTR 30.0%
!! AAAGTGGATTATGTAGGAAA+AGG - chr3_2:83141533-83141552 Msa0431150:three_prime_UTR 30.0%
! ACACTTCTTTTTGGATTCTT+TGG + chr3_2:83141699-83141718 None:intergenic 30.0%
! AGAAGTGTAAAGAATATGGT+AGG - chr3_2:83141711-83141730 Msa0431150:intron 30.0%
TAAGCATAATGTGTTGGATT+TGG + chr3_2:83141995-83142014 None:intergenic 30.0%
ATAAAGAAGGAACAGTTCTT+TGG - chr3_2:83142365-83142384 Msa0431150:CDS 30.0%
CATGATGTCAACTAAGAAAA+TGG + chr3_2:83142477-83142496 None:intergenic 30.0%
! CATTTTCTTAGTTGACATCA+TGG - chr3_2:83142475-83142494 Msa0431150:CDS 30.0%
! CATGTCTTTTTGAATCTCTT+AGG - chr3_2:83142502-83142521 Msa0431150:five_prime_UTR 30.0%
AATGATAGGCAGAATTTGAA+GGG + chr3_2:83142557-83142576 None:intergenic 30.0%
TAATGATAGGCAGAATTTGA+AGG + chr3_2:83142558-83142577 None:intergenic 30.0%
TACAATAGTGAAAGTGAATC+TGG - chr3_2:83141449-83141468 Msa0431150:three_prime_UTR 30.0%
GTTCTTCAGAAAAAGATGAA+TGG + chr3_2:83141493-83141512 None:intergenic 30.0%
GAACATTTCAATAGGAAAAG+TGG - chr3_2:83141509-83141528 Msa0431150:three_prime_UTR 30.0%
!! AAAGTGGATTATGTAGGAAA+AGG - chr3_2:83141533-83141552 Msa0431150:three_prime_UTR 30.0%
! ACACTTCTTTTTGGATTCTT+TGG + chr3_2:83141699-83141718 None:intergenic 30.0%
! AGAAGTGTAAAGAATATGGT+AGG - chr3_2:83141711-83141730 Msa0431150:intron 30.0%
TAAGCATAATGTGTTGGATT+TGG + chr3_2:83141995-83142014 None:intergenic 30.0%
ATAAAGAAGGAACAGTTCTT+TGG - chr3_2:83142365-83142384 Msa0431150:CDS 30.0%
CATGATGTCAACTAAGAAAA+TGG + chr3_2:83142477-83142496 None:intergenic 30.0%
! CATTTTCTTAGTTGACATCA+TGG - chr3_2:83142475-83142494 Msa0431150:CDS 30.0%
! CATGTCTTTTTGAATCTCTT+AGG - chr3_2:83142502-83142521 Msa0431150:five_prime_UTR 30.0%
AATGATAGGCAGAATTTGAA+GGG + chr3_2:83142557-83142576 None:intergenic 30.0%
TAATGATAGGCAGAATTTGA+AGG + chr3_2:83142558-83142577 None:intergenic 30.0%
TGAAAACTAGTGTTGTAGAG+TGG + chr3_2:83141301-83141320 None:intergenic 35.0%
ATAGTGAAAGTGAATCTGGT+TGG - chr3_2:83141453-83141472 Msa0431150:three_prime_UTR 35.0%
AACAATGGAAGAAGAAGAAG+AGG - chr3_2:83141559-83141578 Msa0431150:three_prime_UTR 35.0%
GGAAGAAGAAGATTTATCCA+TGG - chr3_2:83141583-83141602 Msa0431150:three_prime_UTR 35.0%
!! TTAGCTTTGTGAGTTACCTT+GGG + chr3_2:83141794-83141813 None:intergenic 35.0%
!! ATTAGCTTTGTGAGTTACCT+TGG + chr3_2:83141795-83141814 None:intergenic 35.0%
TTCTTGTTGCAGAGAAAGAA+GGG + chr3_2:83141946-83141965 None:intergenic 35.0%
TTTCTCTGCAACAAGAACAA+AGG - chr3_2:83141949-83141968 Msa0431150:intron 35.0%
! AAGCATTCAAGTTTCTTGGA+TGG - chr3_2:83142094-83142113 Msa0431150:intron 35.0%
!! AAAAAGATGCAAGGTGTTGA+AGG - chr3_2:83142326-83142345 Msa0431150:CDS 35.0%
ATGATAGGCAGAATTTGAAG+GGG + chr3_2:83142556-83142575 None:intergenic 35.0%
TGAAAACTAGTGTTGTAGAG+TGG + chr3_2:83141301-83141320 None:intergenic 35.0%
ATAGTGAAAGTGAATCTGGT+TGG - chr3_2:83141453-83141472 Msa0431150:three_prime_UTR 35.0%
AACAATGGAAGAAGAAGAAG+AGG - chr3_2:83141559-83141578 Msa0431150:three_prime_UTR 35.0%
GGAAGAAGAAGATTTATCCA+TGG - chr3_2:83141583-83141602 Msa0431150:three_prime_UTR 35.0%
!! TTAGCTTTGTGAGTTACCTT+GGG + chr3_2:83141794-83141813 None:intergenic 35.0%
!! ATTAGCTTTGTGAGTTACCT+TGG + chr3_2:83141795-83141814 None:intergenic 35.0%
TTCTTGTTGCAGAGAAAGAA+GGG + chr3_2:83141946-83141965 None:intergenic 35.0%
TTTCTCTGCAACAAGAACAA+AGG - chr3_2:83141949-83141968 Msa0431150:intron 35.0%
! AAGCATTCAAGTTTCTTGGA+TGG - chr3_2:83142094-83142113 Msa0431150:intron 35.0%
!! AAAAAGATGCAAGGTGTTGA+AGG - chr3_2:83142326-83142345 Msa0431150:CDS 35.0%
ATGATAGGCAGAATTTGAAG+GGG + chr3_2:83142556-83142575 None:intergenic 35.0%
CTAGTGTTGTAGAGTGGTAA+AGG + chr3_2:83141295-83141314 None:intergenic 40.0%
CAATAGGAAAAGTGGGAAAG+TGG - chr3_2:83141517-83141536 Msa0431150:three_prime_UTR 40.0%
!! AGTGGGAAAGTGGATTATGT+AGG - chr3_2:83141527-83141546 Msa0431150:three_prime_UTR 40.0%
TGTAGGAAAAGGAGCAACAA+TGG - chr3_2:83141544-83141563 Msa0431150:three_prime_UTR 40.0%
AATGGAAGAAGAAGAAGAGG+AGG - chr3_2:83141562-83141581 Msa0431150:three_prime_UTR 40.0%
!! AGAAGAAGCATCAGAAACCA+TGG + chr3_2:83141603-83141622 None:intergenic 40.0%
ATGGTTTCTGATGCTTCTTC+TGG - chr3_2:83141602-83141621 Msa0431150:three_prime_UTR 40.0%
TCTACATGATAGTACTGTGG+AGG + chr3_2:83141629-83141648 None:intergenic 40.0%
TCCACAGTACTATCATGTAG+AGG - chr3_2:83141628-83141647 Msa0431150:three_prime_UTR 40.0%
TCCTCTACATGATAGTACTG+TGG + chr3_2:83141632-83141651 None:intergenic 40.0%
GAACTAGCAGTATCATCAAG+AGG + chr3_2:83141755-83141774 None:intergenic 40.0%
CTTGGGATAGTTAAGAACAG+GGG + chr3_2:83141777-83141796 None:intergenic 40.0%
CCTTGGGATAGTTAAGAACA+GGG + chr3_2:83141778-83141797 None:intergenic 40.0%
ACCTTGGGATAGTTAAGAAC+AGG + chr3_2:83141779-83141798 None:intergenic 40.0%
TTTCTCAGAAAATGGAGCAG+TGG - chr3_2:83141901-83141920 Msa0431150:intron 40.0%
TCTTGTTGCAGAGAAAGAAG+GGG + chr3_2:83141945-83141964 None:intergenic 40.0%
GTTCTTGTTGCAGAGAAAGA+AGG + chr3_2:83141947-83141966 None:intergenic 40.0%
CCAAGAAACTTGAATGCTTC+TGG + chr3_2:83142093-83142112 None:intergenic 40.0%
!! CCAGAAGCATTCAAGTTTCT+TGG - chr3_2:83142090-83142109 Msa0431150:intron 40.0%
! CATTCAAGTTTCTTGGATGG+TGG - chr3_2:83142097-83142116 Msa0431150:intron 40.0%
TGGTGGAAAACAAGCTTCAA+AGG - chr3_2:83142114-83142133 Msa0431150:intron 40.0%
AAACAAGCTTCAAAGGAACC+AGG - chr3_2:83142121-83142140 Msa0431150:intron 40.0%
!!! GTGGTTTTAATGGAGATGAG+AGG - chr3_2:83142245-83142264 Msa0431150:CDS 40.0%
TGCGTCAGAAGCTAAAAGTA+AGG - chr3_2:83142296-83142315 Msa0431150:CDS 40.0%
GCGTCAGAAGCTAAAAGTAA+GGG - chr3_2:83142297-83142316 Msa0431150:CDS 40.0%
GGGATGTGAAAAAAGATGCA+AGG - chr3_2:83142317-83142336 Msa0431150:CDS 40.0%
TACGTTGTTCCAAACTGCAT+TGG + chr3_2:83142432-83142451 None:intergenic 40.0%
! GTTTGGAACAACGTAAGAAC+AGG - chr3_2:83142437-83142456 Msa0431150:CDS 40.0%
CTAGTGTTGTAGAGTGGTAA+AGG + chr3_2:83141295-83141314 None:intergenic 40.0%
CAATAGGAAAAGTGGGAAAG+TGG - chr3_2:83141517-83141536 Msa0431150:three_prime_UTR 40.0%
!! AGTGGGAAAGTGGATTATGT+AGG - chr3_2:83141527-83141546 Msa0431150:three_prime_UTR 40.0%
TGTAGGAAAAGGAGCAACAA+TGG - chr3_2:83141544-83141563 Msa0431150:three_prime_UTR 40.0%
AATGGAAGAAGAAGAAGAGG+AGG - chr3_2:83141562-83141581 Msa0431150:three_prime_UTR 40.0%
!! AGAAGAAGCATCAGAAACCA+TGG + chr3_2:83141603-83141622 None:intergenic 40.0%
ATGGTTTCTGATGCTTCTTC+TGG - chr3_2:83141602-83141621 Msa0431150:three_prime_UTR 40.0%
TCTACATGATAGTACTGTGG+AGG + chr3_2:83141629-83141648 None:intergenic 40.0%
TCCACAGTACTATCATGTAG+AGG - chr3_2:83141628-83141647 Msa0431150:three_prime_UTR 40.0%
TCCTCTACATGATAGTACTG+TGG + chr3_2:83141632-83141651 None:intergenic 40.0%
GAACTAGCAGTATCATCAAG+AGG + chr3_2:83141755-83141774 None:intergenic 40.0%
CTTGGGATAGTTAAGAACAG+GGG + chr3_2:83141777-83141796 None:intergenic 40.0%
CCTTGGGATAGTTAAGAACA+GGG + chr3_2:83141778-83141797 None:intergenic 40.0%
ACCTTGGGATAGTTAAGAAC+AGG + chr3_2:83141779-83141798 None:intergenic 40.0%
TTTCTCAGAAAATGGAGCAG+TGG - chr3_2:83141901-83141920 Msa0431150:intron 40.0%
TCTTGTTGCAGAGAAAGAAG+GGG + chr3_2:83141945-83141964 None:intergenic 40.0%
GTTCTTGTTGCAGAGAAAGA+AGG + chr3_2:83141947-83141966 None:intergenic 40.0%
CCAAGAAACTTGAATGCTTC+TGG + chr3_2:83142093-83142112 None:intergenic 40.0%
!! CCAGAAGCATTCAAGTTTCT+TGG - chr3_2:83142090-83142109 Msa0431150:intron 40.0%
! CATTCAAGTTTCTTGGATGG+TGG - chr3_2:83142097-83142116 Msa0431150:intron 40.0%
TGGTGGAAAACAAGCTTCAA+AGG - chr3_2:83142114-83142133 Msa0431150:intron 40.0%
AAACAAGCTTCAAAGGAACC+AGG - chr3_2:83142121-83142140 Msa0431150:intron 40.0%
!!! GTGGTTTTAATGGAGATGAG+AGG - chr3_2:83142245-83142264 Msa0431150:CDS 40.0%
TGCGTCAGAAGCTAAAAGTA+AGG - chr3_2:83142296-83142315 Msa0431150:CDS 40.0%
GCGTCAGAAGCTAAAAGTAA+GGG - chr3_2:83142297-83142316 Msa0431150:CDS 40.0%
GGGATGTGAAAAAAGATGCA+AGG - chr3_2:83142317-83142336 Msa0431150:CDS 40.0%
TACGTTGTTCCAAACTGCAT+TGG + chr3_2:83142432-83142451 None:intergenic 40.0%
! GTTTGGAACAACGTAAGAAC+AGG - chr3_2:83142437-83142456 Msa0431150:CDS 40.0%
CCCTGTTCTTAACTATCCCA+AGG - chr3_2:83141775-83141794 Msa0431150:intron 45.0%
! TTCCAAACTGCATTGGCAGT+GGG + chr3_2:83142425-83142444 None:intergenic 45.0%
CCCTGTTCTTAACTATCCCA+AGG - chr3_2:83141775-83141794 Msa0431150:intron 45.0%
! TTCCAAACTGCATTGGCAGT+GGG + chr3_2:83142425-83142444 None:intergenic 45.0%
!!! TAATTATAACTAATTTTATT+TGG - chr3_2:83141832-83141851 Msa0431150:intron 5.0%
!!! TAATTATAACTAATTTTATT+TGG - chr3_2:83141832-83141851 Msa0431150:intron 5.0%
! GTTCCAAACTGCATTGGCAG+TGG + chr3_2:83142426-83142445 None:intergenic 50.0%
TGTCGGATTGTCGCTCAGTT+TGG - chr3_2:83142651-83142670 Msa0431150:five_prime_UTR 50.0%
! GTTCCAAACTGCATTGGCAG+TGG + chr3_2:83142426-83142445 None:intergenic 50.0%
TGTCGGATTGTCGCTCAGTT+TGG - chr3_2:83142651-83142670 Msa0431150:five_prime_UTR 50.0%
GTCCCACTGCCAATGCAGTT+TGG - chr3_2:83142420-83142439 Msa0431150:CDS 55.0%
GTCCCACTGCCAATGCAGTT+TGG - chr3_2:83142420-83142439 Msa0431150:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 83141220 83142703 83141220 ID=Msa0431150;Name=Msa0431150
chr3_2 mRNA 83141220 83142703 83141220 ID=Msa0431150-mRNA-1;Parent=Msa0431150;Name=Msa0431150-mRNA-1;_AED=0.07;_eAED=0.07;_QI=205|1|1|1|1|1|4|430|182
chr3_2 exon 83141220 83141678 83141220 ID=Msa0431150-mRNA-1:exon:23167;Parent=Msa0431150-mRNA-1
chr3_2 exon 83141781 83141847 83141781 ID=Msa0431150-mRNA-1:exon:23166;Parent=Msa0431150-mRNA-1
chr3_2 exon 83141953 83142033 83141953 ID=Msa0431150-mRNA-1:exon:23165;Parent=Msa0431150-mRNA-1
chr3_2 exon 83142127 83142703 83142127 ID=Msa0431150-mRNA-1:exon:23164;Parent=Msa0431150-mRNA-1
chr3_2 five_prime_UTR 83142499 83142703 83142499 ID=Msa0431150-mRNA-1:five_prime_utr;Parent=Msa0431150-mRNA-1
chr3_2 CDS 83142127 83142498 83142127 ID=Msa0431150-mRNA-1:cds;Parent=Msa0431150-mRNA-1
chr3_2 CDS 83141953 83142033 83141953 ID=Msa0431150-mRNA-1:cds;Parent=Msa0431150-mRNA-1
chr3_2 CDS 83141781 83141847 83141781 ID=Msa0431150-mRNA-1:cds;Parent=Msa0431150-mRNA-1
chr3_2 CDS 83141650 83141678 83141650 ID=Msa0431150-mRNA-1:cds;Parent=Msa0431150-mRNA-1
chr3_2 three_prime_UTR 83141220 83141649 83141220 ID=Msa0431150-mRNA-1:three_prime_utr;Parent=Msa0431150-mRNA-1
Gene Sequence

>Msa0431150

ATGGATATGTCAAATTATTCAGAATACAATAGTGAAAGTGAATCTGGTTGGACTAATTACTTGAACCATTCATCTTTTTCTGAAGAACATTTCAATAGGAAAAGTGGGAAAGTGGATTATGTAGGAAAAGGAGCAACAATGGAAGAAGAAGAAGAGGAGGAAGAAGAAGATTTATCCATGGTTTCTGATGCTTCTTCTGGACCTCCACAGTACTATCATGTAGAGGAAAATTACCAACAACCTTATTGTGTAAATTGGAATTCTTCAACTTCCAAAGAATCCAAAAAGAAGTGTAAAGAATATGGTAGGAAAAGTCAACAGTCTTCACCTCTTGATGATACTGCTAGTTCCCCTGTTCTTAACTATCCCAAGAATAAAGTCAATTTCTCAGAAAATGGAGCAGTGGAGAATACACTTGATTTTTCCCCTTCTTTCTCTGCAACAAGAACAAAGAGAAAGTCTAAACTCCAGAAGCATTCAAGTTTCTTGGATGGTGGAAAACAAGCTTCAAAGGAACCAGGTGGTTTTAATGGAGATGAGAGGAAATGA
Protein sequence

>Msa0431150

MDMSNYSEYNSESESGWTNYLNHSSFSEEHFNRKSGKVDYVGKGATMEEEEEEEEEDLSMVSDASSGPPQYYHVEENYQQPYCVNWNSSTSKESKKKCKEYGRKSQQSSPLDDTASSPVLNYPKNKVNFSENGAVENTLDFSPSFSATRTKRKSKLQKHSSFLDGGKQASKEPGGFNGDERK*