AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0381290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0381290 MtrunA17_Chr3g0134941 95.652 184 6 1 1 182 1 184 9.71e-127 353
Msa0381290 MtrunA17_Chr1g0152381 50.943 159 60 6 1 148 1 152 6.31e-32 112
Msa0381290 MtrunA17_Chr5g0393821 38.037 163 88 6 7 163 6 161 2.42e-14 67.8
Msa0381290 MtrunA17_Chr1g0207011 34.524 168 94 6 1 155 1 165 5.13e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0381290 AT4G33800.2 35.057 174 77 6 11 163 6 164 2.19e-12 62.4
Msa0381290 AT4G33800.1 35.057 174 77 6 11 163 6 164 2.19e-12 62.4
Msa0381290 AT1G58460.1 34.848 198 91 10 1 182 1 176 5.88e-11 58.9

Find 58 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACCTTATTGTGTAAAT+TGG 0.134932 3_1:+83317522 Msa0381290:CDS
CCAGAAGCATTCAAGTTTCT+TGG 0.140446 3_1:+83317952 Msa0381290:CDS
TAGACAAGTGTTAGTTAAAA+TGG 0.155616 3_1:+83317268 Msa0381290:exon
ACTCTTATATATATAGATTT+TGG 0.190453 3_1:-83317204 None:intergenic
GAATTCCAATTTACACAATA+AGG 0.257445 3_1:-83317527 None:intergenic
CCAAGAAACTTGAATGCTTC+TGG 0.269813 3_1:-83317952 None:intergenic
AAAGTCAATTTCTCAGAAAA+TGG 0.272188 3_1:+83317755 Msa0381290:CDS
ATAAAGAAGGAACAGTTCTT+TGG 0.278886 3_1:+83318227 Msa0381290:three_prime_UTR
ATGGTTTCTGATGCTTCTTC+TGG 0.283239 3_1:+83317464 Msa0381290:CDS
CATGATGTCAACTAAGAAAA+TGG 0.287865 3_1:-83318336 None:intergenic
ACCTTGGGATAGTTAAGAAC+AGG 0.357740 3_1:-83317638 None:intergenic
TACAATAGTGAAAGTGAATC+TGG 0.373922 3_1:+83317311 Msa0381290:CDS
TTTCTGAAGAACATTTCAAT+AGG 0.386107 3_1:+83317363 Msa0381290:CDS
CAAGTGTATATTTAGTTTGT+CGG 0.388292 3_1:+83318496 Msa0381290:three_prime_UTR
AAAAGAAACTCTAGTTCTAT+AGG 0.414355 3_1:+83317130 Msa0381290:five_prime_UTR
AATTTACACAATAAGGTTGT+TGG 0.428691 3_1:-83317520 None:intergenic
TGTCGGATTGTCGCTCAGTT+TGG 0.441637 3_1:+83318513 Msa0381290:three_prime_UTR
GATGAAGTAAACATAATGAT+AGG 0.453876 3_1:-83318430 None:intergenic
GTCCCACTGCCAATGCAGTT+TGG 0.454243 3_1:+83318282 Msa0381290:three_prime_UTR
GTTCTTGTTGCAGAGAAAGA+AGG 0.454355 3_1:-83317806 None:intergenic
TGCGTCAGAAGCTAAAAGTA+AGG 0.464184 3_1:+83318158 Msa0381290:three_prime_UTR
GAACATTTCAATAGGAAAAG+TGG 0.473979 3_1:+83317371 Msa0381290:CDS
CAATAGGAAAAGTGGGAAAG+TGG 0.474045 3_1:+83317379 Msa0381290:CDS
CATTCAAGTTTCTTGGATGG+TGG 0.474914 3_1:+83317959 Msa0381290:CDS
CTAGTGTTGTAGAGTGGTAA+AGG 0.492301 3_1:-83317154 None:intergenic
GTTCCAAACTGCATTGGCAG+TGG 0.494977 3_1:-83318285 None:intergenic
AAAGTGGATTATGTAGGAAA+AGG 0.502120 3_1:+83317395 Msa0381290:CDS
TTCTTGTTGCAGAGAAAGAA+GGG 0.506519 3_1:-83317805 None:intergenic
GTTTGGAACAACGTAAGAAC+AGG 0.508021 3_1:+83318299 Msa0381290:three_prime_UTR
AACATTTCAATAGGAAAAGT+GGG 0.508806 3_1:+83317372 Msa0381290:CDS
TAATGATAGGCAGAATTTGA+AGG 0.517062 3_1:-83318417 None:intergenic
TACGTTGTTCCAAACTGCAT+TGG 0.529331 3_1:-83318291 None:intergenic
AACAATGGAAGAAGAAGAAG+AGG 0.533757 3_1:+83317421 Msa0381290:CDS
AATGATAGGCAGAATTTGAA+GGG 0.538940 3_1:-83318416 None:intergenic
TGGTGGAAAACAAGCTTCAA+AGG 0.539652 3_1:+83317976 Msa0381290:CDS
AAGCATTCAAGTTTCTTGGA+TGG 0.545383 3_1:+83317956 Msa0381290:CDS
CCCTGTTCTTAACTATCCCA+AGG 0.552465 3_1:+83317637 Msa0381290:CDS
AGTGGGAAAGTGGATTATGT+AGG 0.561242 3_1:+83317389 Msa0381290:CDS
AGAAGTGTAAAGAATATGGT+AGG 0.566325 3_1:+83317573 Msa0381290:CDS
GGAAGAAGAAGATTTATCCA+TGG 0.566800 3_1:+83317445 Msa0381290:CDS
TGTAGGAAAAGGAGCAACAA+TGG 0.568877 3_1:+83317406 Msa0381290:CDS
AATGGAAGAAGAAGAAGAGG+AGG 0.569909 3_1:+83317424 Msa0381290:CDS
TTCCAAACTGCATTGGCAGT+GGG 0.575619 3_1:-83318284 None:intergenic
ATAGTGAAAGTGAATCTGGT+TGG 0.584733 3_1:+83317315 Msa0381290:CDS
AGAAGAAGCATCAGAAACCA+TGG 0.595770 3_1:-83317462 None:intergenic
CCTTGGGATAGTTAAGAACA+GGG 0.598514 3_1:-83317637 None:intergenic
GCGTCAGAAGCTAAAAGTAA+GGG 0.607870 3_1:+83318159 Msa0381290:three_prime_UTR
TTTCTCAGAAAATGGAGCAG+TGG 0.613686 3_1:+83317763 Msa0381290:CDS
TTTCTCTGCAACAAGAACAA+AGG 0.615674 3_1:+83317811 Msa0381290:CDS
TCTTGTTGCAGAGAAAGAAG+GGG 0.652031 3_1:-83317804 None:intergenic
AAACAAGCTTCAAAGGAACC+AGG 0.657474 3_1:+83317983 Msa0381290:CDS
TCCTCTACATGATAGTACTG+TGG 0.658500 3_1:-83317491 None:intergenic
GAACTAGCAGTATCATCAAG+AGG 0.659139 3_1:-83317614 None:intergenic
TGAAAACTAGTGTTGTAGAG+TGG 0.666228 3_1:-83317160 None:intergenic
ATGATAGGCAGAATTTGAAG+GGG 0.666686 3_1:-83318415 None:intergenic
TCCACAGTACTATCATGTAG+AGG 0.668253 3_1:+83317490 Msa0381290:CDS
TCTACATGATAGTACTGTGG+AGG 0.671420 3_1:-83317488 None:intergenic
CTTGGGATAGTTAAGAACAG+GGG 0.715448 3_1:-83317636 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATATAGATTTTGGTA+AGG - chr3_1:83317202-83317221 None:intergenic 15.0%
!!! ACTCTTATATATATAGATTT+TGG - chr3_1:83317207-83317226 None:intergenic 15.0%
!!! TTATAACTAATTTTATTTGG+AGG + chr3_1:83317697-83317716 Msa0381290:intron 15.0%
!! ATTAATTATGTATATATACC+TGG - chr3_1:83318004-83318023 None:intergenic 15.0%
!! AAAAATAGAGAAAACAAAAA+GGG - chr3_1:83318456-83318475 None:intergenic 15.0%
!! AAAAAATAGAGAAAACAAAA+AGG - chr3_1:83318457-83318476 None:intergenic 15.0%
!!! TTTGTTTTCTCTATTTTTTT+AGG + chr3_1:83318458-83318477 Msa0381290:three_prime_UTR 15.0%
!!! TATATATATAGATTTTGGTA+AGG - chr3_1:83317202-83317221 None:intergenic 15.0%
!!! ACTCTTATATATATAGATTT+TGG - chr3_1:83317207-83317226 None:intergenic 15.0%
!!! TTATAACTAATTTTATTTGG+AGG + chr3_1:83317697-83317716 Msa0381290:intron 15.0%
!! ATTAATTATGTATATATACC+TGG - chr3_1:83318004-83318023 None:intergenic 15.0%
!! AAAAATAGAGAAAACAAAAA+GGG - chr3_1:83318456-83318475 None:intergenic 15.0%
!! AAAAAATAGAGAAAACAAAA+AGG - chr3_1:83318457-83318476 None:intergenic 15.0%
!!! TTTGTTTTCTCTATTTTTTT+AGG + chr3_1:83318458-83318477 Msa0381290:three_prime_UTR 15.0%
!!! ATATTCTTTACACTTCTTTT+TGG - chr3_1:83317570-83317589 None:intergenic 20.0%
!!! AAAAAGAAGTGTAAAGAATA+TGG + chr3_1:83317569-83317588 Msa0381290:CDS 20.0%
!! ATGCTTATTAGTTATTACAA+TGG + chr3_1:83317870-83317889 Msa0381290:intron 20.0%
!! TTGTTTATGTCTTGATTATA+AGG + chr3_1:83318085-83318104 Msa0381290:intron 20.0%
!! AAGTGATGTAAAAATAAAGA+AGG + chr3_1:83318214-83318233 Msa0381290:three_prime_UTR 20.0%
!! AAAATAGAGAAAACAAAAAG+GGG - chr3_1:83318455-83318474 None:intergenic 20.0%
!!! CTAAATATACACTTGATTTT+TGG - chr3_1:83318491-83318510 None:intergenic 20.0%
!!! ATATTCTTTACACTTCTTTT+TGG - chr3_1:83317570-83317589 None:intergenic 20.0%
!!! AAAAAGAAGTGTAAAGAATA+TGG + chr3_1:83317569-83317588 Msa0381290:CDS 20.0%
!! ATGCTTATTAGTTATTACAA+TGG + chr3_1:83317870-83317889 Msa0381290:intron 20.0%
!! TTGTTTATGTCTTGATTATA+AGG + chr3_1:83318085-83318104 Msa0381290:intron 20.0%
!! AAGTGATGTAAAAATAAAGA+AGG + chr3_1:83318214-83318233 Msa0381290:three_prime_UTR 20.0%
!! AAAATAGAGAAAACAAAAAG+GGG - chr3_1:83318455-83318474 None:intergenic 20.0%
!!! CTAAATATACACTTGATTTT+TGG - chr3_1:83318491-83318510 None:intergenic 20.0%
!!! AAAAGAAACTCTAGTTCTAT+AGG + chr3_1:83317130-83317149 Msa0381290:five_prime_UTR 25.0%
! TAGACAAGTGTTAGTTAAAA+TGG + chr3_1:83317268-83317287 Msa0381290:exon 25.0%
!! TTTCTGAAGAACATTTCAAT+AGG + chr3_1:83317363-83317382 Msa0381290:CDS 25.0%
! AACATTTCAATAGGAAAAGT+GGG + chr3_1:83317372-83317391 Msa0381290:CDS 25.0%
! AATTTACACAATAAGGTTGT+TGG - chr3_1:83317523-83317542 None:intergenic 25.0%
! AACAACCTTATTGTGTAAAT+TGG + chr3_1:83317522-83317541 Msa0381290:CDS 25.0%
! GAATTCCAATTTACACAATA+AGG - chr3_1:83317530-83317549 None:intergenic 25.0%
! AAAGTCAATTTCTCAGAAAA+TGG + chr3_1:83317755-83317774 Msa0381290:CDS 25.0%
! AACTAATAAGCATAATGTGT+TGG - chr3_1:83317863-83317882 None:intergenic 25.0%
!!! TGAATGTTTTAAGCTAATGA+AGG + chr3_1:83318061-83318080 Msa0381290:intron 25.0%
! TTTATGTCTTGATTATAAGG+TGG + chr3_1:83318088-83318107 Msa0381290:intron 25.0%
!!! TGATTATAAGGTGGTTTTAA+TGG + chr3_1:83318097-83318116 Msa0381290:intron 25.0%
! GATGAAGTAAACATAATGAT+AGG - chr3_1:83318433-83318452 None:intergenic 25.0%
!!! CAAGTGTATATTTAGTTTGT+CGG + chr3_1:83318496-83318515 Msa0381290:three_prime_UTR 25.0%
!!! TCTTAATGTGTTTGTTTTCA+AGG - chr3_1:83317084-83317103 None:intergenic 25.0%
!!! AAAAGAAACTCTAGTTCTAT+AGG + chr3_1:83317130-83317149 Msa0381290:five_prime_UTR 25.0%
! TAGACAAGTGTTAGTTAAAA+TGG + chr3_1:83317268-83317287 Msa0381290:exon 25.0%
!! TTTCTGAAGAACATTTCAAT+AGG + chr3_1:83317363-83317382 Msa0381290:CDS 25.0%
! AACATTTCAATAGGAAAAGT+GGG + chr3_1:83317372-83317391 Msa0381290:CDS 25.0%
! AATTTACACAATAAGGTTGT+TGG - chr3_1:83317523-83317542 None:intergenic 25.0%
! AACAACCTTATTGTGTAAAT+TGG + chr3_1:83317522-83317541 Msa0381290:CDS 25.0%
! GAATTCCAATTTACACAATA+AGG - chr3_1:83317530-83317549 None:intergenic 25.0%
! AAAGTCAATTTCTCAGAAAA+TGG + chr3_1:83317755-83317774 Msa0381290:CDS 25.0%
! AACTAATAAGCATAATGTGT+TGG - chr3_1:83317863-83317882 None:intergenic 25.0%
!!! TGAATGTTTTAAGCTAATGA+AGG + chr3_1:83318061-83318080 Msa0381290:intron 25.0%
! TTTATGTCTTGATTATAAGG+TGG + chr3_1:83318088-83318107 Msa0381290:intron 25.0%
!!! TGATTATAAGGTGGTTTTAA+TGG + chr3_1:83318097-83318116 Msa0381290:intron 25.0%
! GATGAAGTAAACATAATGAT+AGG - chr3_1:83318433-83318452 None:intergenic 25.0%
!!! CAAGTGTATATTTAGTTTGT+CGG + chr3_1:83318496-83318515 Msa0381290:three_prime_UTR 25.0%
TACAATAGTGAAAGTGAATC+TGG + chr3_1:83317311-83317330 Msa0381290:CDS 30.0%
GTTCTTCAGAAAAAGATGAA+TGG - chr3_1:83317355-83317374 None:intergenic 30.0%
GAACATTTCAATAGGAAAAG+TGG + chr3_1:83317371-83317390 Msa0381290:CDS 30.0%
!! AAAGTGGATTATGTAGGAAA+AGG + chr3_1:83317395-83317414 Msa0381290:CDS 30.0%
! ACACTTCTTTTTGGATTCTT+TGG - chr3_1:83317561-83317580 None:intergenic 30.0%
! AGAAGTGTAAAGAATATGGT+AGG + chr3_1:83317573-83317592 Msa0381290:CDS 30.0%
TAAGCATAATGTGTTGGATT+TGG - chr3_1:83317857-83317876 None:intergenic 30.0%
ATAAAGAAGGAACAGTTCTT+TGG + chr3_1:83318227-83318246 Msa0381290:three_prime_UTR 30.0%
CATGATGTCAACTAAGAAAA+TGG - chr3_1:83318339-83318358 None:intergenic 30.0%
! CATTTTCTTAGTTGACATCA+TGG + chr3_1:83318337-83318356 Msa0381290:three_prime_UTR 30.0%
! CATGTCTTTTTGAATCTCTT+AGG + chr3_1:83318364-83318383 Msa0381290:three_prime_UTR 30.0%
AATGATAGGCAGAATTTGAA+GGG - chr3_1:83318419-83318438 None:intergenic 30.0%
TAATGATAGGCAGAATTTGA+AGG - chr3_1:83318420-83318439 None:intergenic 30.0%
TACAATAGTGAAAGTGAATC+TGG + chr3_1:83317311-83317330 Msa0381290:CDS 30.0%
GTTCTTCAGAAAAAGATGAA+TGG - chr3_1:83317355-83317374 None:intergenic 30.0%
GAACATTTCAATAGGAAAAG+TGG + chr3_1:83317371-83317390 Msa0381290:CDS 30.0%
!! AAAGTGGATTATGTAGGAAA+AGG + chr3_1:83317395-83317414 Msa0381290:CDS 30.0%
! ACACTTCTTTTTGGATTCTT+TGG - chr3_1:83317561-83317580 None:intergenic 30.0%
! AGAAGTGTAAAGAATATGGT+AGG + chr3_1:83317573-83317592 Msa0381290:CDS 30.0%
TAAGCATAATGTGTTGGATT+TGG - chr3_1:83317857-83317876 None:intergenic 30.0%
ATAAAGAAGGAACAGTTCTT+TGG + chr3_1:83318227-83318246 Msa0381290:three_prime_UTR 30.0%
CATGATGTCAACTAAGAAAA+TGG - chr3_1:83318339-83318358 None:intergenic 30.0%
! CATTTTCTTAGTTGACATCA+TGG + chr3_1:83318337-83318356 Msa0381290:three_prime_UTR 30.0%
! CATGTCTTTTTGAATCTCTT+AGG + chr3_1:83318364-83318383 Msa0381290:three_prime_UTR 30.0%
AATGATAGGCAGAATTTGAA+GGG - chr3_1:83318419-83318438 None:intergenic 30.0%
TAATGATAGGCAGAATTTGA+AGG - chr3_1:83318420-83318439 None:intergenic 30.0%
TGAAAACTAGTGTTGTAGAG+TGG - chr3_1:83317163-83317182 None:intergenic 35.0%
ATAGTGAAAGTGAATCTGGT+TGG + chr3_1:83317315-83317334 Msa0381290:CDS 35.0%
AACAATGGAAGAAGAAGAAG+AGG + chr3_1:83317421-83317440 Msa0381290:CDS 35.0%
GGAAGAAGAAGATTTATCCA+TGG + chr3_1:83317445-83317464 Msa0381290:CDS 35.0%
!! TTAGCTTTGTGAGTTACCTT+GGG - chr3_1:83317656-83317675 None:intergenic 35.0%
!! ATTAGCTTTGTGAGTTACCT+TGG - chr3_1:83317657-83317676 None:intergenic 35.0%
TTCTTGTTGCAGAGAAAGAA+GGG - chr3_1:83317808-83317827 None:intergenic 35.0%
TTTCTCTGCAACAAGAACAA+AGG + chr3_1:83317811-83317830 Msa0381290:CDS 35.0%
! AAGCATTCAAGTTTCTTGGA+TGG + chr3_1:83317956-83317975 Msa0381290:CDS 35.0%
!! AAAAAGATGCAAGGTGTTGA+AGG + chr3_1:83318188-83318207 Msa0381290:three_prime_UTR 35.0%
ATGATAGGCAGAATTTGAAG+GGG - chr3_1:83318418-83318437 None:intergenic 35.0%
TGAAAACTAGTGTTGTAGAG+TGG - chr3_1:83317163-83317182 None:intergenic 35.0%
ATAGTGAAAGTGAATCTGGT+TGG + chr3_1:83317315-83317334 Msa0381290:CDS 35.0%
AACAATGGAAGAAGAAGAAG+AGG + chr3_1:83317421-83317440 Msa0381290:CDS 35.0%
GGAAGAAGAAGATTTATCCA+TGG + chr3_1:83317445-83317464 Msa0381290:CDS 35.0%
!! TTAGCTTTGTGAGTTACCTT+GGG - chr3_1:83317656-83317675 None:intergenic 35.0%
!! ATTAGCTTTGTGAGTTACCT+TGG - chr3_1:83317657-83317676 None:intergenic 35.0%
TTCTTGTTGCAGAGAAAGAA+GGG - chr3_1:83317808-83317827 None:intergenic 35.0%
TTTCTCTGCAACAAGAACAA+AGG + chr3_1:83317811-83317830 Msa0381290:CDS 35.0%
! AAGCATTCAAGTTTCTTGGA+TGG + chr3_1:83317956-83317975 Msa0381290:CDS 35.0%
!! AAAAAGATGCAAGGTGTTGA+AGG + chr3_1:83318188-83318207 Msa0381290:three_prime_UTR 35.0%
ATGATAGGCAGAATTTGAAG+GGG - chr3_1:83318418-83318437 None:intergenic 35.0%
CTAGTGTTGTAGAGTGGTAA+AGG - chr3_1:83317157-83317176 None:intergenic 40.0%
CAATAGGAAAAGTGGGAAAG+TGG + chr3_1:83317379-83317398 Msa0381290:CDS 40.0%
!! AGTGGGAAAGTGGATTATGT+AGG + chr3_1:83317389-83317408 Msa0381290:CDS 40.0%
TGTAGGAAAAGGAGCAACAA+TGG + chr3_1:83317406-83317425 Msa0381290:CDS 40.0%
AATGGAAGAAGAAGAAGAGG+AGG + chr3_1:83317424-83317443 Msa0381290:CDS 40.0%
!! AGAAGAAGCATCAGAAACCA+TGG - chr3_1:83317465-83317484 None:intergenic 40.0%
ATGGTTTCTGATGCTTCTTC+TGG + chr3_1:83317464-83317483 Msa0381290:CDS 40.0%
TCTACATGATAGTACTGTGG+AGG - chr3_1:83317491-83317510 None:intergenic 40.0%
TCCACAGTACTATCATGTAG+AGG + chr3_1:83317490-83317509 Msa0381290:CDS 40.0%
TCCTCTACATGATAGTACTG+TGG - chr3_1:83317494-83317513 None:intergenic 40.0%
GAACTAGCAGTATCATCAAG+AGG - chr3_1:83317617-83317636 None:intergenic 40.0%
CTTGGGATAGTTAAGAACAG+GGG - chr3_1:83317639-83317658 None:intergenic 40.0%
CCTTGGGATAGTTAAGAACA+GGG - chr3_1:83317640-83317659 None:intergenic 40.0%
ACCTTGGGATAGTTAAGAAC+AGG - chr3_1:83317641-83317660 None:intergenic 40.0%
TTTCTCAGAAAATGGAGCAG+TGG + chr3_1:83317763-83317782 Msa0381290:CDS 40.0%
TCTTGTTGCAGAGAAAGAAG+GGG - chr3_1:83317807-83317826 None:intergenic 40.0%
GTTCTTGTTGCAGAGAAAGA+AGG - chr3_1:83317809-83317828 None:intergenic 40.0%
CCAAGAAACTTGAATGCTTC+TGG - chr3_1:83317955-83317974 None:intergenic 40.0%
!! CCAGAAGCATTCAAGTTTCT+TGG + chr3_1:83317952-83317971 Msa0381290:CDS 40.0%
! CATTCAAGTTTCTTGGATGG+TGG + chr3_1:83317959-83317978 Msa0381290:CDS 40.0%
TGGTGGAAAACAAGCTTCAA+AGG + chr3_1:83317976-83317995 Msa0381290:CDS 40.0%
AAACAAGCTTCAAAGGAACC+AGG + chr3_1:83317983-83318002 Msa0381290:CDS 40.0%
!!! GTGGTTTTAATGGAGATGAG+AGG + chr3_1:83318107-83318126 Msa0381290:CDS 40.0%
TGCGTCAGAAGCTAAAAGTA+AGG + chr3_1:83318158-83318177 Msa0381290:three_prime_UTR 40.0%
GCGTCAGAAGCTAAAAGTAA+GGG + chr3_1:83318159-83318178 Msa0381290:three_prime_UTR 40.0%
GGGATGTGAAAAAAGATGCA+AGG + chr3_1:83318179-83318198 Msa0381290:three_prime_UTR 40.0%
TACGTTGTTCCAAACTGCAT+TGG - chr3_1:83318294-83318313 None:intergenic 40.0%
! GTTTGGAACAACGTAAGAAC+AGG + chr3_1:83318299-83318318 Msa0381290:three_prime_UTR 40.0%
CTAGTGTTGTAGAGTGGTAA+AGG - chr3_1:83317157-83317176 None:intergenic 40.0%
CAATAGGAAAAGTGGGAAAG+TGG + chr3_1:83317379-83317398 Msa0381290:CDS 40.0%
!! AGTGGGAAAGTGGATTATGT+AGG + chr3_1:83317389-83317408 Msa0381290:CDS 40.0%
TGTAGGAAAAGGAGCAACAA+TGG + chr3_1:83317406-83317425 Msa0381290:CDS 40.0%
AATGGAAGAAGAAGAAGAGG+AGG + chr3_1:83317424-83317443 Msa0381290:CDS 40.0%
!! AGAAGAAGCATCAGAAACCA+TGG - chr3_1:83317465-83317484 None:intergenic 40.0%
ATGGTTTCTGATGCTTCTTC+TGG + chr3_1:83317464-83317483 Msa0381290:CDS 40.0%
TCTACATGATAGTACTGTGG+AGG - chr3_1:83317491-83317510 None:intergenic 40.0%
TCCACAGTACTATCATGTAG+AGG + chr3_1:83317490-83317509 Msa0381290:CDS 40.0%
TCCTCTACATGATAGTACTG+TGG - chr3_1:83317494-83317513 None:intergenic 40.0%
GAACTAGCAGTATCATCAAG+AGG - chr3_1:83317617-83317636 None:intergenic 40.0%
CTTGGGATAGTTAAGAACAG+GGG - chr3_1:83317639-83317658 None:intergenic 40.0%
CCTTGGGATAGTTAAGAACA+GGG - chr3_1:83317640-83317659 None:intergenic 40.0%
ACCTTGGGATAGTTAAGAAC+AGG - chr3_1:83317641-83317660 None:intergenic 40.0%
TTTCTCAGAAAATGGAGCAG+TGG + chr3_1:83317763-83317782 Msa0381290:CDS 40.0%
TCTTGTTGCAGAGAAAGAAG+GGG - chr3_1:83317807-83317826 None:intergenic 40.0%
GTTCTTGTTGCAGAGAAAGA+AGG - chr3_1:83317809-83317828 None:intergenic 40.0%
CCAAGAAACTTGAATGCTTC+TGG - chr3_1:83317955-83317974 None:intergenic 40.0%
!! CCAGAAGCATTCAAGTTTCT+TGG + chr3_1:83317952-83317971 Msa0381290:CDS 40.0%
! CATTCAAGTTTCTTGGATGG+TGG + chr3_1:83317959-83317978 Msa0381290:CDS 40.0%
TGGTGGAAAACAAGCTTCAA+AGG + chr3_1:83317976-83317995 Msa0381290:CDS 40.0%
AAACAAGCTTCAAAGGAACC+AGG + chr3_1:83317983-83318002 Msa0381290:CDS 40.0%
!!! GTGGTTTTAATGGAGATGAG+AGG + chr3_1:83318107-83318126 Msa0381290:CDS 40.0%
TGCGTCAGAAGCTAAAAGTA+AGG + chr3_1:83318158-83318177 Msa0381290:three_prime_UTR 40.0%
GCGTCAGAAGCTAAAAGTAA+GGG + chr3_1:83318159-83318178 Msa0381290:three_prime_UTR 40.0%
GGGATGTGAAAAAAGATGCA+AGG + chr3_1:83318179-83318198 Msa0381290:three_prime_UTR 40.0%
TACGTTGTTCCAAACTGCAT+TGG - chr3_1:83318294-83318313 None:intergenic 40.0%
! GTTTGGAACAACGTAAGAAC+AGG + chr3_1:83318299-83318318 Msa0381290:three_prime_UTR 40.0%
CCCTGTTCTTAACTATCCCA+AGG + chr3_1:83317637-83317656 Msa0381290:CDS 45.0%
! TTCCAAACTGCATTGGCAGT+GGG - chr3_1:83318287-83318306 None:intergenic 45.0%
CCCTGTTCTTAACTATCCCA+AGG + chr3_1:83317637-83317656 Msa0381290:CDS 45.0%
! TTCCAAACTGCATTGGCAGT+GGG - chr3_1:83318287-83318306 None:intergenic 45.0%
!!! TAATTATAACTAATTTTATT+TGG + chr3_1:83317694-83317713 Msa0381290:intron 5.0%
!!! TAATTATAACTAATTTTATT+TGG + chr3_1:83317694-83317713 Msa0381290:intron 5.0%
! GTTCCAAACTGCATTGGCAG+TGG - chr3_1:83318288-83318307 None:intergenic 50.0%
TGTCGGATTGTCGCTCAGTT+TGG + chr3_1:83318513-83318532 Msa0381290:three_prime_UTR 50.0%
! GTTCCAAACTGCATTGGCAG+TGG - chr3_1:83318288-83318307 None:intergenic 50.0%
TGTCGGATTGTCGCTCAGTT+TGG + chr3_1:83318513-83318532 Msa0381290:three_prime_UTR 50.0%
GTCCCACTGCCAATGCAGTT+TGG + chr3_1:83318282-83318301 Msa0381290:three_prime_UTR 55.0%
GTCCCACTGCCAATGCAGTT+TGG + chr3_1:83318282-83318301 Msa0381290:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 83317082 83318565 83317082 ID=Msa0381290;Name=Msa0381290
chr3_1 mRNA 83317082 83318565 83317082 ID=Msa0381290-mRNA-1;Parent=Msa0381290;Name=Msa0381290-mRNA-1;_AED=0.07;_eAED=0.07;_QI=205|1|1|1|1|1|4|430|182
chr3_1 exon 83317082 83317658 83317082 ID=Msa0381290-mRNA-1:exon:21392;Parent=Msa0381290-mRNA-1
chr3_1 exon 83317752 83317832 83317752 ID=Msa0381290-mRNA-1:exon:21393;Parent=Msa0381290-mRNA-1
chr3_1 exon 83317938 83318004 83317938 ID=Msa0381290-mRNA-1:exon:21394;Parent=Msa0381290-mRNA-1
chr3_1 exon 83318107 83318565 83318107 ID=Msa0381290-mRNA-1:exon:21395;Parent=Msa0381290-mRNA-1
chr3_1 five_prime_UTR 83317082 83317286 83317082 ID=Msa0381290-mRNA-1:five_prime_utr;Parent=Msa0381290-mRNA-1
chr3_1 CDS 83317287 83317658 83317287 ID=Msa0381290-mRNA-1:cds;Parent=Msa0381290-mRNA-1
chr3_1 CDS 83317752 83317832 83317752 ID=Msa0381290-mRNA-1:cds;Parent=Msa0381290-mRNA-1
chr3_1 CDS 83317938 83318004 83317938 ID=Msa0381290-mRNA-1:cds;Parent=Msa0381290-mRNA-1
chr3_1 CDS 83318107 83318135 83318107 ID=Msa0381290-mRNA-1:cds;Parent=Msa0381290-mRNA-1
chr3_1 three_prime_UTR 83318136 83318565 83318136 ID=Msa0381290-mRNA-1:three_prime_utr;Parent=Msa0381290-mRNA-1
Gene Sequence

>Msa0381290

ATGGATATGTCAAATTATTCACAATACAATAGTGAAAGTGAATCTGGTTGGACTAATTACTTGAACCATTCATCTTTTTCTGAAGAACATTTCAATAGGAAAAGTGGGAAAGTGGATTATGTAGGAAAAGGAGCAACAATGGAAGAAGAAGAAGAGGAGGAAGAAGAAGATTTATCCATGGTTTCTGATGCTTCTTCTGGACCTCCACAGTACTATCATGTAGAGGAAAATTACCAACAACCTTATTGTGTAAATTGGAATTCTTCAACTTCCAAAGAATCCAAAAAGAAGTGTAAAGAATATGGTAGGAAAAGTCAACAGTCTTCACCTCTTGATGATACTGCTAGTTCCCCTGTTCTTAACTATCCCAAGAATAAAGTCAATTTCTCAGAAAATGGAGCAGTGGAGAATACACTTGATTTTTCCCCTTCTTTCTCTGCAACAAGAACAAAGAGAAAGTCTAAACTCCAGAAGCATTCAAGTTTCTTGGATGGTGGAAAACAAGCTTCAAAGGAACCAGGTGGTTTTAATGGAGATGAGAGGAAATGA
Protein sequence

>Msa0381290

MDMSNYSQYNSESESGWTNYLNHSSFSEEHFNRKSGKVDYVGKGATMEEEEEEEEEDLSMVSDASSGPPQYYHVEENYQQPYCVNWNSSTSKESKKKCKEYGRKSQQSSPLDDTASSPVLNYPKNKVNFSENGAVENTLDFSPSFSATRTKRKSKLQKHSSFLDGGKQASKEPGGFNGDERK*