AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0436480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0436480 MtrunA17_Chr8g0387721 99.057 106 1 0 1 106 1 106 5.44e-75 216
Msa0436480 MtrunA17_Chr8g0342041 97.170 106 3 0 1 106 1 106 7.10e-74 214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0436480 AT5G55140.1 62.857 105 38 1 3 106 1 105 1.88e-44 140

Find 87 sgRNAs with CRISPR-Local

Find 277 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGCAATGTTTGCTGATA+TGG 0.161816 3_2:+90052161 Msa0436480:three_prime_UTR
ACTTAGGCATTTGAAGATTT+TGG 0.175275 3_2:-90052195 None:intergenic
GTCATAATATAGTAAATTAT+TGG 0.225889 3_2:+90052508 Msa0436480:three_prime_UTR
AGGCCGGCGGCGCTAGGGTT+GGG 0.259210 3_2:-90049170 None:intergenic
AGTGCACCTATGTTGCTTTC+TGG 0.265924 3_2:-90051843 None:intergenic
AACGCTAGTGAGGGGTATTC+CGG 0.301510 3_2:+90051817 Msa0436480:CDS
GGCGCTAGGGTTGGGGTTGA+AGG 0.302792 3_2:-90049162 None:intergenic
GGTTTCCTCCTTCTCTTTGC+GGG 0.349900 3_2:-90052077 None:intergenic
ACAGTTTATCAATTTCAGTT+TGG 0.359298 3_2:+90052340 Msa0436480:three_prime_UTR
TCTTCAAATGCCTAAGTTTG+AGG 0.363190 3_2:+90052201 Msa0436480:three_prime_UTR
TGCGGTGGTTCCGTCAAAAC+GGG 0.378616 3_2:-90049211 None:intergenic
GTTTGAGGTTGTGCCTCATA+TGG 0.383938 3_2:+90052216 Msa0436480:three_prime_UTR
TCAACCTGCTTCTTCTGCTT+AGG 0.384301 3_2:+90052135 Msa0436480:CDS
CAACCTGCTTCTTCTGCTTA+GGG 0.390606 3_2:+90052136 Msa0436480:CDS
CGCTAGGGTTGGGGTTGAAG+GGG 0.397527 3_2:-90049160 None:intergenic
GATGGAACACACCTACTGTT+AGG 0.400025 3_2:+90051909 Msa0436480:CDS
CACTAGCGTTATGTAGAGAT+TGG 0.401453 3_2:-90051804 None:intergenic
GAGGCCGGCGGCGCTAGGGT+TGG 0.409277 3_2:-90049171 None:intergenic
ACGCTAGTGAGGGGTATTCC+GGG 0.415196 3_2:+90051818 Msa0436480:CDS
GGGTTTCCTCCTTCTCTTTG+CGG 0.418532 3_2:-90052078 None:intergenic
AGATTGGGGCTATAAGCAAT+TGG 0.427253 3_2:-90051788 None:intergenic
CATGAGCATGAATGCGTTCA+AGG 0.431873 3_2:+90051748 Msa0436480:exon
CTTTCAACCAACTCCACTCC+GGG 0.433958 3_2:-90049109 None:intergenic
AAGCAGAAGAAGCAGGTTGA+TGG 0.435151 3_2:-90052132 None:intergenic
GGCCGGCGGCGCTAGGGTTG+GGG 0.438610 3_2:-90049169 None:intergenic
AAGTAGAGGCCGGCGGCGCT+AGG 0.459350 3_2:-90049176 None:intergenic
ACTAGCGTTATGTAGAGATT+GGG 0.460548 3_2:-90051803 None:intergenic
ATGGAACACACCTACTGTTA+GGG 0.464145 3_2:+90051910 Msa0436480:CDS
AGAGAAGAGACGTAAAATAG+AGG 0.464708 3_2:+90049076 Msa0436480:five_prime_UTR
GTTAGTTGTTGTTGAGACAG+AGG 0.465463 3_2:+90052042 Msa0436480:CDS
TGTACGAATAGACTGAATTT+CGG 0.468402 3_2:+90049034 Msa0436480:five_prime_UTR
ATGCGGTGGTTCCGTCAAAA+CGG 0.475445 3_2:-90049212 None:intergenic
GAGAAGAGACGTAAAATAGA+GGG 0.475475 3_2:+90049077 Msa0436480:five_prime_UTR
AAAGCAAATAAAACCATATG+AGG 0.477102 3_2:-90052229 None:intergenic
CTATGTTGCTTTCTGGTCCC+CGG 0.490539 3_2:-90051836 None:intergenic
GCGCTAGGGTTGGGGTTGAA+GGG 0.493852 3_2:-90049161 None:intergenic
CAGAGCTCTTCGTCCTCCAT+TGG 0.509479 3_2:+90052102 Msa0436480:CDS
TGTTCCATCGCATGACAGTT+CGG 0.511917 3_2:-90051895 None:intergenic
AGTAGAGGCCGGCGGCGCTA+GGG 0.513498 3_2:-90049175 None:intergenic
TGGTTCCGTCAAAACGGGGA+AGG 0.514256 3_2:-90049206 None:intergenic
GCTTTCAACCAACTCCACTC+CGG 0.516574 3_2:-90049110 None:intergenic
AACCCCAACCCTAGCGCCGC+CGG 0.521091 3_2:+90049167 Msa0436480:five_prime_UTR
GGTTGATGGTTTATGACCAA+TGG 0.522943 3_2:-90052118 None:intergenic
GAAGTGACCCGGAGTGGAGT+TGG 0.525746 3_2:+90049102 Msa0436480:five_prime_UTR
CTCTAAGTACAACTTAGATT+AGG 0.526741 3_2:-90049007 None:intergenic
GATTGGGGCTATAAGCAATT+GGG 0.527244 3_2:-90051787 None:intergenic
TGTATTTGATATAGGTCAAG+AGG 0.544788 3_2:+90052020 Msa0436480:intron
GGTTCCGTCAAAACGGGGAA+GGG 0.549299 3_2:-90049205 None:intergenic
GTACCCTAAGCAGAAGAAGC+AGG 0.555500 3_2:-90052139 None:intergenic
GTACAAGGCCCGCAAAGAGA+AGG 0.555978 3_2:+90052069 Msa0436480:CDS
AAAGCAACATAGGTGCACTT+TGG 0.556523 3_2:+90051847 Msa0436480:CDS
CGTCAAAACGGGGAAGGGCA+AGG 0.564587 3_2:-90049200 None:intergenic
ATAGACTGAATTTCGGAGAA+GGG 0.567788 3_2:+90049041 Msa0436480:five_prime_UTR
TGGAGGACGAAGAGCTCTGT+GGG 0.570519 3_2:-90052098 None:intergenic
ATCTAAGTTGTACTTAGAGA+TGG 0.572654 3_2:+90049011 Msa0436480:five_prime_UTR
AGAAGAGACGTAAAATAGAG+GGG 0.574408 3_2:+90049078 Msa0436480:five_prime_UTR
AGGGCAAGGAAAGTAGAGGC+CGG 0.576902 3_2:-90049186 None:intergenic
AATAGAGGGGAGAAGTGACC+CGG 0.577040 3_2:+90049091 Msa0436480:five_prime_UTR
AATAGACTGAATTTCGGAGA+AGG 0.579412 3_2:+90049040 Msa0436480:five_prime_UTR
CGGGGACCAGAAAGCAACAT+AGG 0.579617 3_2:+90051837 Msa0436480:CDS
AGGGGAACGCTGATTTGAGA+AGG 0.583542 3_2:-90049142 None:intergenic
TGTTAGGGGAATGATCCAGC+AGG 0.585091 3_2:+90051925 Msa0436480:CDS
TGAGGCACAACCTCAAACTT+AGG 0.586101 3_2:-90052211 None:intergenic
GCTATAAGCAATTGGGACAC+AGG 0.590348 3_2:-90051780 None:intergenic
TGACGGAACCACCGCATAGG+AGG 0.593538 3_2:+90049218 Msa0436480:five_prime_UTR
CCATATCAGCAAACATTGCA+AGG 0.597443 3_2:-90052161 None:intergenic
GCAACCGAACTGTCATGCGA+TGG 0.602415 3_2:+90051891 Msa0436480:CDS
CAAGGCCCGCAAAGAGAAGG+AGG 0.604236 3_2:+90052072 Msa0436480:CDS
ATCTCTACATAACGCTAGTG+AGG 0.609634 3_2:+90051807 Msa0436480:CDS
CTAGCGTTATGTAGAGATTG+GGG 0.616077 3_2:-90051802 None:intergenic
TGGATCATTCCCCTAACAGT+AGG 0.620579 3_2:-90051920 None:intergenic
TGATGGTTTATGACCAATGG+AGG 0.622285 3_2:-90052115 None:intergenic
CAGTTCGGTTGCATTTGCCG+AGG 0.626107 3_2:-90051880 None:intergenic
TCTCTACATAACGCTAGTGA+GGG 0.627577 3_2:+90051808 Msa0436480:CDS
AGGGGAGAAGTGACCCGGAG+TGG 0.637707 3_2:+90049096 Msa0436480:five_prime_UTR
GGAACGCTGATTTGAGAAGG+AGG 0.641765 3_2:-90049139 None:intergenic
TGAGACAGAGGAGATGTACA+AGG 0.642473 3_2:+90052054 Msa0436480:CDS
GCAAGGAAAGTAGAGGCCGG+CGG 0.651496 3_2:-90049183 None:intergenic
TGGAACACACCTACTGTTAG+GGG 0.654422 3_2:+90051911 Msa0436480:CDS
GGGAAGGGCAAGGAAAGTAG+AGG 0.662057 3_2:-90049190 None:intergenic
ATGGAGGACGAAGAGCTCTG+TGG 0.663844 3_2:-90052099 None:intergenic
CGCTAGTGAGGGGTATTCCG+GGG 0.667158 3_2:+90051819 Msa0436480:CDS
AAGACTTACCTCCTATGCGG+TGG 0.667701 3_2:-90049226 None:intergenic
CTCTACATAACGCTAGTGAG+GGG 0.670443 3_2:+90051809 Msa0436480:CDS
ACTTTGGAAGCACTACGCCT+CGG 0.674356 3_2:+90051863 Msa0436480:CDS
GCGGTGGTTCCGTCAAAACG+GGG 0.711779 3_2:-90049210 None:intergenic
TCAAAGACTTACCTCCTATG+CGG 0.743924 3_2:-90049229 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAATGATTGATATAATA+TGG + chr3_2:90050367-90050386 Msa0436480:intron 10.0%
!! AAAATTTAAATAAAATTGGA+AGG - chr3_2:90051616-90051635 None:intergenic 10.0%
!!! TTATGCTTTTATTAATTCAA+GGG + chr3_2:90049328-90049347 Msa0436480:intron 15.0%
!!! TTTTTTTTTTTTACTATAGC+TGG + chr3_2:90049572-90049591 Msa0436480:intron 15.0%
!! AAATAAAAAAATAAAGAACG+TGG - chr3_2:90050850-90050869 None:intergenic 15.0%
!!! TTTTTTAGTAATAAGTCATA+AGG + chr3_2:90051025-90051044 Msa0436480:intron 15.0%
!! GTCATAATATAGTAAATTAT+TGG + chr3_2:90052508-90052527 Msa0436480:three_prime_UTR 15.0%
!!! GTTATGCTTTTATTAATTCA+AGG + chr3_2:90049327-90049346 Msa0436480:intron 20.0%
!! TTTATTATGAAGCATGAATT+GGG - chr3_2:90049930-90049949 None:intergenic 20.0%
!!! TTTTATTATGAAGCATGAAT+TGG - chr3_2:90049931-90049950 None:intergenic 20.0%
!!! AAATAAACAACTCCATTTTA+TGG - chr3_2:90050589-90050608 None:intergenic 20.0%
!!! TAAAATGGAGTTGTTTATTT+TGG + chr3_2:90050589-90050608 Msa0436480:intron 20.0%
!!! ATTTTTATTCCCATGAAATA+GGG + chr3_2:90050793-90050812 Msa0436480:intron 20.0%
!! TAAAAAAAAAATCAAGAACG+TGG - chr3_2:90050913-90050932 None:intergenic 20.0%
!!! TATGTTAATTTTTACAACCT+GGG - chr3_2:90050994-90051013 None:intergenic 20.0%
!!! TTATGTTAATTTTTACAACC+TGG - chr3_2:90050995-90051014 None:intergenic 20.0%
!!! GAATTTTTAAGAAAAGTGTT+TGG - chr3_2:90051108-90051127 None:intergenic 20.0%
!!! ATAATGAGAATTTTATTCCT+AGG - chr3_2:90051130-90051149 None:intergenic 20.0%
!! ATCTGATAAAAAAACATTCT+TGG + chr3_2:90052486-90052505 Msa0436480:three_prime_UTR 20.0%
! TTTCATACTTGATTGATTGA+TGG + chr3_2:90049277-90049296 Msa0436480:intron 25.0%
! CTAAATGATTAATCTCATAC+TGG - chr3_2:90049403-90049422 None:intergenic 25.0%
!! CATTTTCTTAAATTACTGTC+AGG - chr3_2:90049452-90049471 None:intergenic 25.0%
! TAATGAAGAAAACAAAAGCA+AGG - chr3_2:90049683-90049702 None:intergenic 25.0%
!! TTTAACATAACATCGATTGA+TGG + chr3_2:90049885-90049904 Msa0436480:intron 25.0%
! TAGTATATACTGAAACTGAA+AGG - chr3_2:90049958-90049977 None:intergenic 25.0%
! TATATACTATCGTTGAATAG+CGG + chr3_2:90049969-90049988 Msa0436480:intron 25.0%
!!! GTATTTTTTGAAATGTCATG+AGG + chr3_2:90050452-90050471 Msa0436480:intron 25.0%
! TCTTAATATAACAATCCGAT+CGG + chr3_2:90050493-90050512 Msa0436480:intron 25.0%
! ATAAAAATGACGAGAATGTA+AGG - chr3_2:90050781-90050800 None:intergenic 25.0%
!! CATTTTTATTCCCATGAAAT+AGG + chr3_2:90050792-90050811 Msa0436480:intron 25.0%
!! CACTTTTCTTAAAAATTCCT+AGG + chr3_2:90051110-90051129 Msa0436480:intron 25.0%
!! AAATTCTCATTATGTTTTCC+TGG + chr3_2:90051137-90051156 Msa0436480:intron 25.0%
!! AATTCTCATTATGTTTTCCT+GGG + chr3_2:90051138-90051157 Msa0436480:intron 25.0%
! GAACTAAAAAATTGTATTGG+AGG + chr3_2:90051358-90051377 Msa0436480:intron 25.0%
! TAAAAAATTGTATTGGAGGA+GGG + chr3_2:90051362-90051381 Msa0436480:intron 25.0%
! GGTAACTTTGTATTTGATAT+AGG + chr3_2:90052012-90052031 Msa0436480:intron 25.0%
! AAAGCAAATAAAACCATATG+AGG - chr3_2:90052232-90052251 None:intergenic 25.0%
! ACAGTTTATCAATTTCAGTT+TGG + chr3_2:90052340-90052359 Msa0436480:three_prime_UTR 25.0%
CTCTAAGTACAACTTAGATT+AGG - chr3_2:90049010-90049029 None:intergenic 30.0%
ATCTAAGTTGTACTTAGAGA+TGG + chr3_2:90049011-90049030 Msa0436480:five_prime_UTR 30.0%
TGTACGAATAGACTGAATTT+CGG + chr3_2:90049034-90049053 Msa0436480:five_prime_UTR 30.0%
AACAACCTAAACCTTAACAT+TGG - chr3_2:90049305-90049324 None:intergenic 30.0%
!! ATGTTAAGGTTTAGGTTGTT+TGG + chr3_2:90049305-90049324 Msa0436480:intron 30.0%
! TGCTTTTATTAATTCAAGGG+AGG + chr3_2:90049331-90049350 Msa0436480:intron 30.0%
! CTAATCATATGTGTTGATGT+TGG + chr3_2:90049484-90049503 Msa0436480:intron 30.0%
!!! AATTTGCTGCCTATGTTTTA+TGG - chr3_2:90049539-90049558 None:intergenic 30.0%
TCTTCATTAAAACTTCAGCA+AGG + chr3_2:90049694-90049713 Msa0436480:intron 30.0%
!! GCTTTTGTTGTGCTATATTT+CGG + chr3_2:90049822-90049841 Msa0436480:intron 30.0%
!! TTGTTTTTCAGTTAGTTGTC+AGG + chr3_2:90050179-90050198 Msa0436480:intron 30.0%
! ATGCATCAGTTGTCAATTTT+AGG + chr3_2:90050233-90050252 Msa0436480:intron 30.0%
! AGTACAACTTTTAGTATGGA+AGG + chr3_2:90050259-90050278 Msa0436480:intron 30.0%
! TGAACCAATTTAAAACCGAT+CGG - chr3_2:90050511-90050530 None:intergenic 30.0%
AAAACTCAAAACTAGTGTGA+GGG + chr3_2:90050532-90050551 Msa0436480:intron 30.0%
AGAGATAAAAAACACATTCC+TGG + chr3_2:90050663-90050682 Msa0436480:intron 30.0%
GAGATAAAAAACACATTCCT+GGG + chr3_2:90050664-90050683 Msa0436480:intron 30.0%
GAAAGAAGAATACCTATGTT+TGG + chr3_2:90050699-90050718 Msa0436480:intron 30.0%
AATACCTATGTTTGGTAGAA+AGG + chr3_2:90050707-90050726 Msa0436480:intron 30.0%
GGAACAAAAATAATATGCCT+AGG + chr3_2:90050728-90050747 Msa0436480:intron 30.0%
AAAAAAATCAAGAACGTGGT+TGG - chr3_2:90050909-90050928 None:intergenic 30.0%
! ATGAGTTTTTATCAAGAACG+TGG - chr3_2:90050946-90050965 None:intergenic 30.0%
TGGGAACACAATAGTAAAAT+TGG - chr3_2:90050975-90050994 None:intergenic 30.0%
! AATTTTACTATTGTGTTCCC+AGG + chr3_2:90050974-90050993 Msa0436480:intron 30.0%
! AGGATTCTTTTCAAAGAAGT+GGG - chr3_2:90051067-90051086 None:intergenic 30.0%
! TAGGATTCTTTTCAAAGAAG+TGG - chr3_2:90051068-90051087 None:intergenic 30.0%
! ACTTCTTTGAAAAGAATCCT+AGG + chr3_2:90051067-90051086 Msa0436480:intron 30.0%
! GGTTGTCAATTTTTATTCCT+AGG - chr3_2:90051087-90051106 None:intergenic 30.0%
TGGAAAAAATAGAGGACATT+GGG + chr3_2:90051276-90051295 Msa0436480:intron 30.0%
!!! ATTTTTTAGTTCCCCCAAAT+GGG - chr3_2:90051350-90051369 None:intergenic 30.0%
!!! AATTTTTTAGTTCCCCCAAA+TGG - chr3_2:90051351-90051370 None:intergenic 30.0%
GGGGAACTAAAAAATTGTAT+TGG + chr3_2:90051355-90051374 Msa0436480:intron 30.0%
CTAAAAAATTGTATTGGAGG+AGG + chr3_2:90051361-90051380 Msa0436480:intron 30.0%
AAATTGGAAGGAAAACACTT+TGG - chr3_2:90051604-90051623 None:intergenic 30.0%
ACCAATGTTGTTAGTATACA+GGG - chr3_2:90051995-90052014 None:intergenic 30.0%
TACCAATGTTGTTAGTATAC+AGG - chr3_2:90051996-90052015 None:intergenic 30.0%
TGTATTTGATATAGGTCAAG+AGG + chr3_2:90052020-90052039 Msa0436480:intron 30.0%
! ACTTAGGCATTTGAAGATTT+TGG - chr3_2:90052198-90052217 None:intergenic 30.0%
AATAGACTGAATTTCGGAGA+AGG + chr3_2:90049040-90049059 Msa0436480:five_prime_UTR 35.0%
ATAGACTGAATTTCGGAGAA+GGG + chr3_2:90049041-90049060 Msa0436480:five_prime_UTR 35.0%
AGAGAAGAGACGTAAAATAG+AGG + chr3_2:90049076-90049095 Msa0436480:five_prime_UTR 35.0%
GAGAAGAGACGTAAAATAGA+GGG + chr3_2:90049077-90049096 Msa0436480:five_prime_UTR 35.0%
AGAAGAGACGTAAAATAGAG+GGG + chr3_2:90049078-90049097 Msa0436480:five_prime_UTR 35.0%
! GATTGATGGATCCAATGTTA+AGG + chr3_2:90049291-90049310 Msa0436480:intron 35.0%
TGGATCCAATGTTAAGGTTT+AGG + chr3_2:90049297-90049316 Msa0436480:intron 35.0%
TTATGGACACTTCTGATTGA+AGG - chr3_2:90049522-90049541 None:intergenic 35.0%
!! CAGAAGTGTCCATAAAACAT+AGG + chr3_2:90049527-90049546 Msa0436480:intron 35.0%
CCAATTTAAGCATATGTTGC+AGG - chr3_2:90049769-90049788 None:intergenic 35.0%
CCTGCAACATATGCTTAAAT+TGG + chr3_2:90049766-90049785 Msa0436480:intron 35.0%
!! CTCTGTCATCTTTTGTTTAG+TGG + chr3_2:90049800-90049819 Msa0436480:intron 35.0%
! GTGCAGTACAACTTTTAGTA+TGG + chr3_2:90050255-90050274 Msa0436480:intron 35.0%
!!! CAATCCGATCGGTTTTAAAT+TGG + chr3_2:90050504-90050523 Msa0436480:intron 35.0%
CAAAACTCAAAACTAGTGTG+AGG + chr3_2:90050531-90050550 Msa0436480:intron 35.0%
AAACTCAAAACTAGTGTGAG+GGG + chr3_2:90050533-90050552 Msa0436480:intron 35.0%
! AACAACTCCATTTTATGGTC+CGG - chr3_2:90050584-90050603 None:intergenic 35.0%
TTGGTTCAGTTAATTGAACC+AGG + chr3_2:90050608-90050627 Msa0436480:intron 35.0%
TGTTCCTTTCTACCAAACAT+AGG - chr3_2:90050714-90050733 None:intergenic 35.0%
TGTAAGGTAAGTAGATTCCT+GGG - chr3_2:90050765-90050784 None:intergenic 35.0%
ATGTAAGGTAAGTAGATTCC+TGG - chr3_2:90050766-90050785 None:intergenic 35.0%
TTATTCCCATGAAATAGGGA+TGG + chr3_2:90050797-90050816 Msa0436480:intron 35.0%
TATTCCCATGAAATAGGGAT+GGG + chr3_2:90050798-90050817 Msa0436480:intron 35.0%
! TTTTCCCATCCCTATTTCAT+GGG - chr3_2:90050805-90050824 None:intergenic 35.0%
! ATTTTCCCATCCCTATTTCA+TGG - chr3_2:90050806-90050825 None:intergenic 35.0%
GATGGGAAAATTATATTCCC+AGG + chr3_2:90050815-90050834 Msa0436480:intron 35.0%
! GTTTTTATCAAGAACGTGGT+TGG - chr3_2:90050942-90050961 None:intergenic 35.0%
! ATTCTTTTCAAAGAAGTGGG+TGG - chr3_2:90051064-90051083 None:intergenic 35.0%
GAAAAAGATGAAGGAGTAAG+TGG + chr3_2:90051256-90051275 Msa0436480:intron 35.0%
GGAGTAAGTGGAAAAAATAG+AGG + chr3_2:90051268-90051287 Msa0436480:intron 35.0%
GTGGAAAAAATAGAGGACAT+TGG + chr3_2:90051275-90051294 Msa0436480:intron 35.0%
AAAAAATAGAGGACATTGGG+AGG + chr3_2:90051279-90051298 Msa0436480:intron 35.0%
AATATAAAACCCTCCCCATT+TGG + chr3_2:90051333-90051352 Msa0436480:intron 35.0%
ATATAAAACCCTCCCCATTT+GGG + chr3_2:90051334-90051353 Msa0436480:intron 35.0%
!!! TTTTTTAGTTCCCCCAAATG+GGG - chr3_2:90051349-90051368 None:intergenic 35.0%
ACTAGCGTTATGTAGAGATT+GGG - chr3_2:90051806-90051825 None:intergenic 35.0%
GGAGATAGGTAGTTTCAAAA+TGG - chr3_2:90051974-90051993 None:intergenic 35.0%
TTGTTAGTATACAGGGAGAT+AGG - chr3_2:90051988-90052007 None:intergenic 35.0%
TCCCTGTATACTAACAACAT+TGG + chr3_2:90051991-90052010 Msa0436480:intron 35.0%
TCTTCAAATGCCTAAGTTTG+AGG + chr3_2:90052201-90052220 Msa0436480:three_prime_UTR 35.0%
!! TAAACTGTTTTCAAGCACAG+AGG - chr3_2:90052328-90052347 None:intergenic 35.0%
TCAAAGACTTACCTCCTATG+CGG - chr3_2:90049232-90049251 None:intergenic 40.0%
! TATGTGTTGATGTTGGACAC+CGG + chr3_2:90049491-90049510 Msa0436480:intron 40.0%
TTGTGAAGTAGCTGCTATAG+CGG + chr3_2:90050061-90050080 Msa0436480:intron 40.0%
TCGCATATTGCAGAAAAGAG+CGG - chr3_2:90050123-90050142 None:intergenic 40.0%
! GAAGGCATAACTGTGATGTT+TGG + chr3_2:90050277-90050296 Msa0436480:intron 40.0%
! GAAAGCATAACCGTGATGTT+TGG + chr3_2:90050399-90050418 Msa0436480:intron 40.0%
TACAGTGACACCAAACATCA+CGG - chr3_2:90050412-90050431 None:intergenic 40.0%
AGGCATGAACCAATCTCTAA+GGG + chr3_2:90050628-90050647 Msa0436480:intron 40.0%
AAACACATTCCTGGGAATAG+AGG + chr3_2:90050672-90050691 Msa0436480:intron 40.0%
CCTGGGCATTAATTATTCCT+AGG - chr3_2:90050748-90050767 None:intergenic 40.0%
CCTAGGAATAATTAATGCCC+AGG + chr3_2:90050745-90050764 Msa0436480:intron 40.0%
TTCCTGGGAAAGTTAATTCC+AGG + chr3_2:90051153-90051172 Msa0436480:intron 40.0%
TCCTGGGAAAGTTAATTCCA+GGG + chr3_2:90051154-90051173 Msa0436480:intron 40.0%
! CAGGGAGTAATTTTTGCATC+CGG + chr3_2:90051172-90051191 Msa0436480:intron 40.0%
! AGGGAGTAATTTTTGCATCC+GGG + chr3_2:90051173-90051192 Msa0436480:intron 40.0%
AACTCGCAAAGAAAGCCTTA+GGG - chr3_2:90051210-90051229 None:intergenic 40.0%
TAAGGCTTTCTTTGCGAGTT+CGG + chr3_2:90051210-90051229 Msa0436480:intron 40.0%
GAGGGTTTGGAAAAAGATGA+AGG + chr3_2:90051247-90051266 Msa0436480:intron 40.0%
TATAAAACCCTCCCCATTTG+GGG + chr3_2:90051335-90051354 Msa0436480:intron 40.0%
! ATTGTATTGGAGGAGGGTTT+TGG + chr3_2:90051368-90051387 Msa0436480:intron 40.0%
! TTGTATTGGAGGAGGGTTTT+GGG + chr3_2:90051369-90051388 Msa0436480:intron 40.0%
CTGTGGGGTGAGAAATATAT+GGG - chr3_2:90051542-90051561 None:intergenic 40.0%
TCTGTGGGGTGAGAAATATA+TGG - chr3_2:90051543-90051562 None:intergenic 40.0%
TTTGGCATTGTTTGCGAGTT+TGG - chr3_2:90051586-90051605 None:intergenic 40.0%
CTGTTCTGACTTCAACTTGT+AGG + chr3_2:90051725-90051744 Msa0436480:intron 40.0%
GATTGGGGCTATAAGCAATT+GGG - chr3_2:90051790-90051809 None:intergenic 40.0%
AGATTGGGGCTATAAGCAAT+TGG - chr3_2:90051791-90051810 None:intergenic 40.0%
CTAGCGTTATGTAGAGATTG+GGG - chr3_2:90051805-90051824 None:intergenic 40.0%
CACTAGCGTTATGTAGAGAT+TGG - chr3_2:90051807-90051826 None:intergenic 40.0%
ATCTCTACATAACGCTAGTG+AGG + chr3_2:90051807-90051826 Msa0436480:CDS 40.0%
TCTCTACATAACGCTAGTGA+GGG + chr3_2:90051808-90051827 Msa0436480:CDS 40.0%
AAAGCAACATAGGTGCACTT+TGG + chr3_2:90051847-90051866 Msa0436480:CDS 40.0%
ATGGAACACACCTACTGTTA+GGG + chr3_2:90051910-90051929 Msa0436480:CDS 40.0%
! GTTAGTTGTTGTTGAGACAG+AGG + chr3_2:90052042-90052061 Msa0436480:CDS 40.0%
TGATGGTTTATGACCAATGG+AGG - chr3_2:90052118-90052137 None:intergenic 40.0%
!! GGTTGATGGTTTATGACCAA+TGG - chr3_2:90052121-90052140 None:intergenic 40.0%
CCATATCAGCAAACATTGCA+AGG - chr3_2:90052164-90052183 None:intergenic 40.0%
! CCTTGCAATGTTTGCTGATA+TGG + chr3_2:90052161-90052180 Msa0436480:three_prime_UTR 40.0%
TGTGCTATATTTCGGCAAGC+AGG + chr3_2:90049830-90049849 Msa0436480:intron 45.0%
! CTTAGAGATTGGTTCATGCC+TGG - chr3_2:90050629-90050648 None:intergenic 45.0%
CAGGCATGAACCAATCTCTA+AGG + chr3_2:90050627-90050646 Msa0436480:intron 45.0%
GGCATGAACCAATCTCTAAG+GGG + chr3_2:90050629-90050648 Msa0436480:intron 45.0%
! GCATGAACCAATCTCTAAGG+GGG + chr3_2:90050630-90050649 Msa0436480:intron 45.0%
ACATTCCTGGGAATAGAGGT+TGG + chr3_2:90050676-90050695 Msa0436480:intron 45.0%
CATTCCTGGGAATAGAGGTT+GGG + chr3_2:90050677-90050696 Msa0436480:intron 45.0%
TCCCTGGAATTAACTTTCCC+AGG - chr3_2:90051158-90051177 None:intergenic 45.0%
CGGATGCAAAAATTACTCCC+TGG - chr3_2:90051174-90051193 None:intergenic 45.0%
ACTCGCAAAGAAAGCCTTAG+GGG - chr3_2:90051209-90051228 None:intergenic 45.0%
GAACTCGCAAAGAAAGCCTT+AGG - chr3_2:90051211-90051230 None:intergenic 45.0%
ATAAAACCCTCCCCATTTGG+GGG + chr3_2:90051336-90051355 Msa0436480:intron 45.0%
! TGTATTGGAGGAGGGTTTTG+GGG + chr3_2:90051370-90051389 Msa0436480:intron 45.0%
CATTGTTTGCGAGTTTGGAG+GGG - chr3_2:90051581-90051600 None:intergenic 45.0%
GCATTGTTTGCGAGTTTGGA+GGG - chr3_2:90051582-90051601 None:intergenic 45.0%
CATGAGCATGAATGCGTTCA+AGG + chr3_2:90051748-90051767 Msa0436480:exon 45.0%
GCTATAAGCAATTGGGACAC+AGG - chr3_2:90051783-90051802 None:intergenic 45.0%
CTCTACATAACGCTAGTGAG+GGG + chr3_2:90051809-90051828 Msa0436480:CDS 45.0%
!! AGTGCACCTATGTTGCTTTC+TGG - chr3_2:90051846-90051865 None:intergenic 45.0%
TGTTCCATCGCATGACAGTT+CGG - chr3_2:90051898-90051917 None:intergenic 45.0%
GATGGAACACACCTACTGTT+AGG + chr3_2:90051909-90051928 Msa0436480:CDS 45.0%
TGGAACACACCTACTGTTAG+GGG + chr3_2:90051911-90051930 Msa0436480:CDS 45.0%
TGGATCATTCCCCTAACAGT+AGG - chr3_2:90051923-90051942 None:intergenic 45.0%
TGAGACAGAGGAGATGTACA+AGG + chr3_2:90052054-90052073 Msa0436480:CDS 45.0%
! AAGCAGAAGAAGCAGGTTGA+TGG - chr3_2:90052135-90052154 None:intergenic 45.0%
TCAACCTGCTTCTTCTGCTT+AGG + chr3_2:90052135-90052154 Msa0436480:CDS 45.0%
CAACCTGCTTCTTCTGCTTA+GGG + chr3_2:90052136-90052155 Msa0436480:CDS 45.0%
TGAGGCACAACCTCAAACTT+AGG - chr3_2:90052214-90052233 None:intergenic 45.0%
! GTTTGAGGTTGTGCCTCATA+TGG + chr3_2:90052216-90052235 Msa0436480:three_prime_UTR 45.0%
!! CAATATATTATATATAAATA+TGG + chr3_2:90050147-90050166 Msa0436480:intron 5.0%
!! AGATAAAATTTAAATAAAAT+TGG - chr3_2:90051620-90051639 None:intergenic 5.0%
AATAGAGGGGAGAAGTGACC+CGG + chr3_2:90049091-90049110 Msa0436480:five_prime_UTR 50.0%
CTTTCAACCAACTCCACTCC+GGG - chr3_2:90049112-90049131 None:intergenic 50.0%
GCTTTCAACCAACTCCACTC+CGG - chr3_2:90049113-90049132 None:intergenic 50.0%
!! GGAACGCTGATTTGAGAAGG+AGG - chr3_2:90049142-90049161 None:intergenic 50.0%
!! AGGGGAACGCTGATTTGAGA+AGG - chr3_2:90049145-90049164 None:intergenic 50.0%
ATGCGGTGGTTCCGTCAAAA+CGG - chr3_2:90049215-90049234 None:intergenic 50.0%
! TTTTGACGGAACCACCGCAT+AGG + chr3_2:90049215-90049234 Msa0436480:five_prime_UTR 50.0%
AAGACTTACCTCCTATGCGG+TGG - chr3_2:90049229-90049248 None:intergenic 50.0%
! CTTCTGATTGAAGGCGTGTC+CGG - chr3_2:90049513-90049532 None:intergenic 50.0%
CTCAAAACTAGTGTGAGGGG+AGG + chr3_2:90050536-90050555 Msa0436480:intron 50.0%
CTAGTGTGAGGGGAGGAAAA+TGG + chr3_2:90050543-90050562 Msa0436480:intron 50.0%
ATGGCTCAGTTTGCGAAAGC+CGG + chr3_2:90050562-90050581 Msa0436480:intron 50.0%
GCGAAAGCCGGACCATAAAA+TGG + chr3_2:90050574-90050593 Msa0436480:intron 50.0%
ACAAACGCCCCCTTAGAGAT+TGG - chr3_2:90050640-90050659 None:intergenic 50.0%
CTTTCCCAACCTCTATTCCC+AGG - chr3_2:90050684-90050703 None:intergenic 50.0%
! GAACGTGGCACTCTTTTCCT+GGG - chr3_2:90050835-90050854 None:intergenic 50.0%
! AGAACGTGGCACTCTTTTCC+TGG - chr3_2:90050836-90050855 None:intergenic 50.0%
!! CTTAGGGGGTGTTTGTTTCC+CGG - chr3_2:90051194-90051213 None:intergenic 50.0%
CTCGCAAAGAAAGCCTTAGG+GGG - chr3_2:90051208-90051227 None:intergenic 50.0%
GCTTTCTTTGCGAGTTCGGA+GGG + chr3_2:90051214-90051233 Msa0436480:intron 50.0%
CTTTCTTTGCGAGTTCGGAG+GGG + chr3_2:90051215-90051234 Msa0436480:intron 50.0%
ATAGAGGACATTGGGAGGAG+AGG + chr3_2:90051284-90051303 Msa0436480:intron 50.0%
TAGAGGACATTGGGAGGAGA+GGG + chr3_2:90051285-90051304 Msa0436480:intron 50.0%
TTAGTTCCCCCAAATGGGGA+GGG - chr3_2:90051345-90051364 None:intergenic 50.0%
! TTTAGTTCCCCCAAATGGGG+AGG - chr3_2:90051346-90051365 None:intergenic 50.0%
!! GTATTGGAGGAGGGTTTTGG+GGG + chr3_2:90051371-90051390 Msa0436480:intron 50.0%
!!! TATTGGAGGAGGGTTTTGGG+GGG + chr3_2:90051372-90051391 Msa0436480:intron 50.0%
TTTGCGAGTTTGGAGGGGAA+GGG - chr3_2:90051576-90051595 None:intergenic 50.0%
GGCATTGTTTGCGAGTTTGG+AGG - chr3_2:90051583-90051602 None:intergenic 50.0%
!! AACGCTAGTGAGGGGTATTC+CGG + chr3_2:90051817-90051836 Msa0436480:CDS 50.0%
!! CTATGTTGCTTTCTGGTCCC+CGG - chr3_2:90051839-90051858 None:intergenic 50.0%
!! ACTTTGGAAGCACTACGCCT+CGG + chr3_2:90051863-90051882 Msa0436480:CDS 50.0%
TGTTAGGGGAATGATCCAGC+AGG + chr3_2:90051925-90051944 Msa0436480:CDS 50.0%
GATCTTGTGCTGAAACCTGC+TGG - chr3_2:90051943-90051962 None:intergenic 50.0%
GGTTTCCTCCTTCTCTTTGC+GGG - chr3_2:90052080-90052099 None:intergenic 50.0%
GGGTTTCCTCCTTCTCTTTG+CGG - chr3_2:90052081-90052100 None:intergenic 50.0%
GTACCCTAAGCAGAAGAAGC+AGG - chr3_2:90052142-90052161 None:intergenic 50.0%
AGGGCAAGGAAAGTAGAGGC+CGG - chr3_2:90049189-90049208 None:intergenic 55.0%
GGGAAGGGCAAGGAAAGTAG+AGG - chr3_2:90049193-90049212 None:intergenic 55.0%
! CTTGCCCTTCCCCGTTTTGA+CGG + chr3_2:90049201-90049220 Msa0436480:five_prime_UTR 55.0%
GGTTCCGTCAAAACGGGGAA+GGG - chr3_2:90049208-90049227 None:intergenic 55.0%
TGGTTCCGTCAAAACGGGGA+AGG - chr3_2:90049209-90049228 None:intergenic 55.0%
TGCGGTGGTTCCGTCAAAAC+GGG - chr3_2:90049214-90049233 None:intergenic 55.0%
!! GGTGTCGACGTGTAAGTGTG+AGG - chr3_2:90049431-90049450 None:intergenic 55.0%
GCAAGCAGGTCTGAATCTGC+TGG + chr3_2:90049844-90049863 Msa0436480:intron 55.0%
CGGGAAACAAACACCCCCTA+AGG + chr3_2:90051192-90051211 Msa0436480:intron 55.0%
GGCTTTCTTTGCGAGTTCGG+AGG + chr3_2:90051213-90051232 Msa0436480:intron 55.0%
TTTGCGAGTTCGGAGGGGAA+GGG + chr3_2:90051220-90051239 Msa0436480:intron 55.0%
! TTGGGAGGAGAGGGCTTTGA+CGG + chr3_2:90051294-90051313 Msa0436480:intron 55.0%
!!! AGGAGGGTTTTGGGGGGTTT+GGG + chr3_2:90051378-90051397 Msa0436480:intron 55.0%
AAGGGAAGGAAGGGCTCTGT+GGG - chr3_2:90051558-90051577 None:intergenic 55.0%
TTGGAGGGGAAGGGAAGGAA+GGG - chr3_2:90051567-90051586 None:intergenic 55.0%
TTTGGAGGGGAAGGGAAGGA+AGG - chr3_2:90051568-90051587 None:intergenic 55.0%
GTTTGCGAGTTTGGAGGGGA+AGG - chr3_2:90051577-90051596 None:intergenic 55.0%
!! ACGCTAGTGAGGGGTATTCC+GGG + chr3_2:90051818-90051837 Msa0436480:CDS 55.0%
CGGGGACCAGAAAGCAACAT+AGG + chr3_2:90051837-90051856 Msa0436480:CDS 55.0%
CAGTTCGGTTGCATTTGCCG+AGG - chr3_2:90051883-90051902 None:intergenic 55.0%
GCAACCGAACTGTCATGCGA+TGG + chr3_2:90051891-90051910 Msa0436480:CDS 55.0%
GTACAAGGCCCGCAAAGAGA+AGG + chr3_2:90052069-90052088 Msa0436480:CDS 55.0%
TGGAGGACGAAGAGCTCTGT+GGG - chr3_2:90052101-90052120 None:intergenic 55.0%
ATGGAGGACGAAGAGCTCTG+TGG - chr3_2:90052102-90052121 None:intergenic 55.0%
CAGAGCTCTTCGTCCTCCAT+TGG + chr3_2:90052102-90052121 Msa0436480:CDS 55.0%
GAAGTGACCCGGAGTGGAGT+TGG + chr3_2:90049102-90049121 Msa0436480:five_prime_UTR 60.0%
CGCTAGGGTTGGGGTTGAAG+GGG - chr3_2:90049163-90049182 None:intergenic 60.0%
GCGCTAGGGTTGGGGTTGAA+GGG - chr3_2:90049164-90049183 None:intergenic 60.0%
GCAAGGAAAGTAGAGGCCGG+CGG - chr3_2:90049186-90049205 None:intergenic 60.0%
CGTCAAAACGGGGAAGGGCA+AGG - chr3_2:90049203-90049222 None:intergenic 60.0%
GCGGTGGTTCCGTCAAAACG+GGG - chr3_2:90049213-90049232 None:intergenic 60.0%
TGACGGAACCACCGCATAGG+AGG + chr3_2:90049218-90049237 Msa0436480:five_prime_UTR 60.0%
CTTTGCGAGTTCGGAGGGGA+AGG + chr3_2:90051219-90051238 Msa0436480:intron 60.0%
GAGTTCGGAGGGGAAGGGAA+TGG + chr3_2:90051225-90051244 Msa0436480:intron 60.0%
TTCGGAGGGGAAGGGAATGG+AGG + chr3_2:90051228-90051247 Msa0436480:intron 60.0%
TCGGAGGGGAAGGGAATGGA+GGG + chr3_2:90051229-90051248 Msa0436480:intron 60.0%
GGGGAAGGGAATGGAGGGTT+TGG + chr3_2:90051234-90051253 Msa0436480:intron 60.0%
! TGGGAGGAGAGGGCTTTGAC+GGG + chr3_2:90051295-90051314 Msa0436480:intron 60.0%
!!! GAGGAGGGTTTTGGGGGGTT+TGG + chr3_2:90051377-90051396 Msa0436480:intron 60.0%
!!! AGGGTTTTGGGGGGTTTGGG+AGG + chr3_2:90051381-90051400 Msa0436480:intron 60.0%
!!! GGGTTTTGGGGGGTTTGGGA+GGG + chr3_2:90051382-90051401 Msa0436480:intron 60.0%
AGGGAAGGAAGGGCTCTGTG+GGG - chr3_2:90051557-90051576 None:intergenic 60.0%
GAAGGGAAGGAAGGGCTCTG+TGG - chr3_2:90051559-90051578 None:intergenic 60.0%
CGAGTTTGGAGGGGAAGGGA+AGG - chr3_2:90051572-90051591 None:intergenic 60.0%
!! CGCTAGTGAGGGGTATTCCG+GGG + chr3_2:90051819-90051838 Msa0436480:CDS 60.0%
CAAGGCCCGCAAAGAGAAGG+AGG + chr3_2:90052072-90052091 Msa0436480:CDS 60.0%
AGGGGAGAAGTGACCCGGAG+TGG + chr3_2:90049096-90049115 Msa0436480:five_prime_UTR 65.0%
GGCGCTAGGGTTGGGGTTGA+AGG - chr3_2:90049165-90049184 None:intergenic 65.0%
AACCCCAACCCTAGCGCCGC+CGG + chr3_2:90049167-90049186 Msa0436480:five_prime_UTR 70.0%
AGTAGAGGCCGGCGGCGCTA+GGG - chr3_2:90049178-90049197 None:intergenic 70.0%
AAGTAGAGGCCGGCGGCGCT+AGG - chr3_2:90049179-90049198 None:intergenic 70.0%
AGGCCGGCGGCGCTAGGGTT+GGG - chr3_2:90049173-90049192 None:intergenic 75.0%
! GGCCGGCGGCGCTAGGGTTG+GGG - chr3_2:90049172-90049191 None:intergenic 80.0%
! GAGGCCGGCGGCGCTAGGGT+TGG - chr3_2:90049174-90049193 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 90048988 90052538 90048988 ID=Msa0436480;Name=Msa0436480
chr3_2 mRNA 90048988 90052538 90048988 ID=Msa0436480-mRNA-1;Parent=Msa0436480;Name=Msa0436480-mRNA-1;_AED=0.05;_eAED=0.05;_QI=254|1|1|1|0.5|0.66|3|382|106
chr3_2 exon 90048988 90049239 90048988 ID=Msa0436480-mRNA-1:exon:26751;Parent=Msa0436480-mRNA-1
chr3_2 exon 90051747 90051946 90051747 ID=Msa0436480-mRNA-1:exon:26752;Parent=Msa0436480-mRNA-1
chr3_2 exon 90052034 90052538 90052034 ID=Msa0436480-mRNA-1:exon:26753;Parent=Msa0436480-mRNA-1
chr3_2 five_prime_UTR 90048988 90049239 90048988 ID=Msa0436480-mRNA-1:five_prime_utr;Parent=Msa0436480-mRNA-1
chr3_2 five_prime_UTR 90051747 90051748 90051747 ID=Msa0436480-mRNA-1:five_prime_utr;Parent=Msa0436480-mRNA-1
chr3_2 CDS 90051749 90051946 90051749 ID=Msa0436480-mRNA-1:cds;Parent=Msa0436480-mRNA-1
chr3_2 CDS 90052034 90052156 90052034 ID=Msa0436480-mRNA-1:cds;Parent=Msa0436480-mRNA-1
chr3_2 three_prime_UTR 90052157 90052538 90052157 ID=Msa0436480-mRNA-1:three_prime_utr;Parent=Msa0436480-mRNA-1
Gene Sequence

>Msa0436480

ATGAGCATGAATGCGTTCAAGGCATATAAGTCCTGTGTCCCAATTGCTTATAGCCCCAATCTCTACATAACGCTAGTGAGGGGTATTCCGGGGACCAGAAAGCAACATAGGTGCACTTTGGAAGCACTACGCCTCGGCAAATGCAACCGAACTGTCATGCGATGGAACACACCTACTGTTAGGGGAATGATCCAGCAGGTCAAGAGGTTAGTTGTTGTTGAGACAGAGGAGATGTACAAGGCCCGCAAAGAGAAGGAGGAAACCCACAGAGCTCTTCGTCCTCCATTGGTCATAAACCATCAACCTGCTTCTTCTGCTTAG
Protein sequence

>Msa0436480

MSMNAFKAYKSCVPIAYSPNLYITLVRGIPGTRKQHRCTLEALRLGKCNRTVMRWNTPTVRGMIQQVKRLVVVETEEMYKARKEKEETHRALRPPLVINHQPASSA*