AlfalfaGEDB | Msa1287370

M. sativa cultivar zhongmu-4 / Msa1287370

KEGG: K12897
GO: -
PFAM: PF00076.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1287370	XP_003629227.2	99.257	269	2	0	67	335	2	270	0.0	518

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1287370	sp\|Q84TH4\|SR45A_ARATH	64.545	110	37	1	116	223	62	171	3.79e-31	124

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1287370	I3T029	99.257	269	2	0	67	335	2	270	0.0	518

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0142220	Msa1287370	-0.802876	4.556430e-49	-8.615850e-47
Msa0223410	Msa1287370	0.806741	7.098228e-50	-8.615850e-47
Msa0344690	Msa1287370	0.801494	8.767212e-49	-8.615850e-47
Msa0375630	Msa1287370	-0.818637	1.767158e-52	-8.615850e-47
Msa0436480	Msa1287370	0.804518	2.078347e-49	-8.615850e-47
Msa0469940	Msa1287370	0.805768	1.138232e-49	-8.615850e-47
Msa0532760	Msa1287370	0.825043	5.813728e-54	-8.615850e-47
Msa0537720	Msa1287370	-0.800147	1.651994e-48	-8.615850e-47
Msa0826320	Msa1287370	-0.808814	2.573996e-50	-8.615850e-47
Msa0834660	Msa1287370	-0.812167	4.858190e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1287370	MtrunA17_Chr8g0371201	99.257	269	2	0	67	335	2	270	0.0	518
Msa1287370	MtrunA17_Chr5g0406171	54.667	300	73	5	58	335	45	303	5.58e-83	253
Msa1287370	MtrunA17_Chr3g0103761	60.274	73	29	0	121	193	39	111	1.34e-21	94.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1287370	AT4G35785.2	86.139	101	14	0	124	224	69	169	1.90e-57	186
Msa1287370	AT4G35785.3	86.076	79	11	0	124	202	69	147	3.27e-44	151
Msa1287370	AT4G35785.6	86.076	79	11	0	124	202	69	147	3.27e-44	151
Msa1287370	AT4G35785.1	86.076	79	11	0	124	202	69	147	8.39e-44	149
Msa1287370	AT4G35785.5	86.076	79	11	0	124	202	69	147	1.15e-43	150
Msa1287370	AT4G35785.4	86.076	79	11	0	124	202	83	161	2.30e-43	149
Msa1287370	AT1G07350.1	64.545	110	37	1	116	223	62	171	3.85e-32	124
Msa1287370	AT1G07350.3	64.045	89	30	1	116	202	32	120	8.89e-31	113
Msa1287370	AT1G07350.2	64.045	89	30	1	116	202	32	120	8.89e-31	113
Msa1287370	AT1G07350.6	63.043	92	32	1	116	205	32	123	1.99e-30	115

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Find 116 sgRNAs with CRISPR-Local

Find 629 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGCTGTGGTGAATGATCG+TGG	0.649760	8_3:-37693700	None:intergenic
AAGCAAAACCACGAGAAATT+CGG	0.345373	8_3:-37692449	None:intergenic
AAGCTTAAAAGTTGTTGAAA+AGG	0.425063	8_3:+37683139	Msa1287370:CDS
AATCGAAAATTTAAAACACT+TGG	0.508340	8_3:-37694009	None:intergenic
AATTCCTTAGATGCCAACAA+AGG	0.633808	8_3:-37694061	None:intergenic
AATTCTCTGGTGGCCGAAAA+TGG	0.307340	8_3:+37682916	Msa1287370:CDS
ACAACGTTCAGCATCCTCAT+GGG	0.618967	8_3:-37692487	None:intergenic
ACAACTACTCTTAGTTCATC+GGG	0.514765	8_3:-37682953	None:intergenic
AGCAAAACCACGAGAAATTC+GGG	0.387981	8_3:-37692448	None:intergenic
AGCGTTCACCCTACGTTGGG+AGG	0.588183	8_3:+37693717	Msa1287370:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATATTACTCTACACTGATA+TGG	+	chr8_3:37682687-37682706	Msa1287370:intron	30.0%
AATAAAATAAGGTGAGATGG+TGG	-	chr8_3:37682849-37682868	None:intergenic	30.0%
TAGTAAAAAGAATTCTCTGG+TGG	+	chr8_3:37682906-37682925	Msa1287370:CDS	30.0%
CATGAATGTCAAAATTCTGA+AGG	-	chr8_3:37682979-37682998	None:intergenic	30.0%
TTACAGGTAGGTGTTTATTA+AGG	+	chr8_3:37683070-37683089	Msa1287370:intron	30.0%
TAAACTTCAGATATGACACA+TGG	+	chr8_3:37683104-37683123	Msa1287370:intron	30.0%
TCAGATTTGACTATTCATGT+TGG	+	chr8_3:37683255-37683274	Msa1287370:intron	30.0%
TTGACTATTCATGTTGGATT+TGG	+	chr8_3:37683261-37683280	Msa1287370:intron	30.0%
CAGAAAAGGAAAAATACCTT+TGG	-	chr8_3:37683364-37683383	None:intergenic	30.0%
GAATCTTTCACCGAAAAAAT+AGG	-	chr8_3:37683488-37683507	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	37682537	37694197	37682537	ID=Msa1287370;Name=Msa1287370
chr8_3	mRNA	37682537	37694197	37682537	ID=Msa1287370-mRNA-1;Parent=Msa1287370;Name=Msa1287370-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0\|0.66\|0.7\|0.7\|1\|1\|10\|380\|335
chr8_3	exon	37682537	37682565	37682537	ID=Msa1287370-mRNA-1:exon:10808;Parent=Msa1287370-mRNA-1
chr8_3	exon	37682877	37682998	37682877	ID=Msa1287370-mRNA-1:exon:10809;Parent=Msa1287370-mRNA-1
chr8_3	exon	37683114	37683160	37683114	ID=Msa1287370-mRNA-1:exon:10810;Parent=Msa1287370-mRNA-1
chr8_3	exon	37691255	37691274	37691255	ID=Msa1287370-mRNA-1:exon:10811;Parent=Msa1287370-mRNA-1
chr8_3	exon	37691822	37691932	37691822	ID=Msa1287370-mRNA-1:exon:10812;Parent=Msa1287370-mRNA-1
chr8_3	exon	37692077	37692100	37692077	ID=Msa1287370-mRNA-1:exon:10813;Parent=Msa1287370-mRNA-1
chr8_3	exon	37692207	37692309	37692207	ID=Msa1287370-mRNA-1:exon:10814;Parent=Msa1287370-mRNA-1
chr8_3	exon	37692414	37692563	37692414	ID=Msa1287370-mRNA-1:exon:10815;Parent=Msa1287370-mRNA-1
chr8_3	exon	37693314	37693383	37693314	ID=Msa1287370-mRNA-1:exon:10816;Parent=Msa1287370-mRNA-1
chr8_3	exon	37693486	37694197	37693486	ID=Msa1287370-mRNA-1:exon:10817;Parent=Msa1287370-mRNA-1
chr8_3	CDS	37682537	37682565	37682537	ID=Msa1287370-mRNA-1:cds;Parent=Msa1287370-mRNA-1
chr8_3	CDS	37682877	37682998	37682877	ID=Msa1287370-mRNA-1:cds;Parent=Msa1287370-mRNA-1
chr8_3	CDS	37683114	37683160	37683114	ID=Msa1287370-mRNA-1:cds;Parent=Msa1287370-mRNA-1
chr8_3	CDS	37691255	37691274	37691255	ID=Msa1287370-mRNA-1:cds;Parent=Msa1287370-mRNA-1
chr8_3	CDS	37691822	37691932	37691822	ID=Msa1287370-mRNA-1:cds;Parent=Msa1287370-mRNA-1
chr8_3	CDS	37692077	37692100	37692077	ID=Msa1287370-mRNA-1:cds;Parent=Msa1287370-mRNA-1
chr8_3	CDS	37692207	37692309	37692207	ID=Msa1287370-mRNA-1:cds;Parent=Msa1287370-mRNA-1
chr8_3	CDS	37692414	37692563	37692414	ID=Msa1287370-mRNA-1:cds;Parent=Msa1287370-mRNA-1
chr8_3	CDS	37693314	37693383	37693314	ID=Msa1287370-mRNA-1:cds;Parent=Msa1287370-mRNA-1
chr8_3	CDS	37693486	37693817	37693486	ID=Msa1287370-mRNA-1:cds;Parent=Msa1287370-mRNA-1
chr8_3	three_prime_UTR	37693818	37694197	37693818	ID=Msa1287370-mRNA-1:three_prime_utr;Parent=Msa1287370-mRNA-1

Gene Sequence

>Msa1287370

ATGGAAGTCAAGTTTGAAGACTTTTTCAGCACATCATGGAAGAAGAACAAGAGCACAATAGTAAAAAGAATTCTCTGGTGGCCGAAAATGGTATCACCAATGGTGCCCGATGAACTAAGAGTAGTTGTCCTTCAGAATTTTGACATTCATGATATGACACATGGAGTGATTCCCAAAAGCTTAAAAGTTGTTGAAAAGGCTGATTCACCTCCTCCAAGGAATTCAAGGTCCCCTTCACCTTCACCATGGAAAGCTCAATCGAGATCTAGATCAAGGTCGAGGTCTGTTCCAAGACAAAGGCCAAGATCCGACTCCAGAGGCCGTGGCAGATCAAGGTCTCGAAGTCCTGTAAGGGAGGTCAATGCTACAAATCCTGGTAATACACTATATGTTACCGGTCTATCTTCAAGGGTCACCGACAGAGACCTAGAGGACCATTTTTCCAAGGAGGGAAAAGTTGCTTCATGTTTTCTTGTGGTGGAGCCACGTACCCGAATTTCTCGTGGTTTTGCTTTTGTTACAATGGATTCCCATGAGGATGCTGAACGTTGTATCAAGCATCTCAATCAATCAGTTTTAGAGGGTCGTTACATCACTGTAGAACGGTCAAGAAGAAAGCGTGCAAGAACTCCAACACCAGGACACTATCTTGGGCTGAAAAATACTAGAGACTATGGCCCTCGTGGTGATCATCGCAGTGACCATCGTGGCGACTTTCGTGGTGACCATCGTGGTGACTTTCGTGGTGATCATCGTGGCGACTATCGCAGTGACCATCGTGGTGACTATCGCGGTGACTACCGAGGTGATCGGGGTAGGAATCGTGGTGGTTCTGGTCGTGCAGATTATTCAGATCGTAGGTCTCCAAGGCGTTCACCATACCGTGGAGGCCACGATCATTCACCACAGCGTTCACCCTACGTTGGGAGGTCTAGGAGGGAGAGATCTAGGTCCATTCCCCGTTCTCCTTATAGCCCAGACAGGAGGTATGCTGCTGGATCTAGGTGA

Protein sequence

>Msa1287370

MEVKFEDFFSTSWKKNKSTIVKRILWWPKMVSPMVPDELRVVVLQNFDIHDMTHGVIPKSLKVVEKADSPPPRNSRSPSPSPWKAQSRSRSRSRSVPRQRPRSDSRGRGRSRSRSPVREVNATNPGNTLYVTGLSSRVTDRDLEDHFSKEGKVASCFLVVEPRTRISRGFAFVTMDSHEDAERCIKHLNQSVLEGRYITVERSRRKRARTPTPGHYLGLKNTRDYGPRGDHRSDHRGDFRGDHRGDFRGDHRGDYRSDHRGDYRGDYRGDRGRNRGGSGRADYSDRRSPRRSPYRGGHDHSPQRSPYVGRSRRERSRSIPRSPYSPDRRYAAGSR*