AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1380010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380010 MtrunA17_Chr8g0387721 99.057 106 1 0 1 106 1 106 5.44e-75 216
Msa1380010 MtrunA17_Chr8g0342041 97.170 106 3 0 1 106 1 106 7.10e-74 214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380010 AT5G55140.1 62.857 105 38 1 3 106 1 105 1.88e-44 140

Find 88 sgRNAs with CRISPR-Local

Find 275 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGCAATGTTTGCTGATA+TGG 0.161816 tig0020315:+144544 Msa1380010:three_prime_UTR
ACTTAGGCATTTGAAGATTT+TGG 0.175275 tig0020315:-144578 None:intergenic
AGGCCGGCGGCGCTAGGGTT+GGG 0.259210 tig0020315:-141553 None:intergenic
AGTGCACCTATGTTGCTTTC+TGG 0.265924 tig0020315:-144226 None:intergenic
AACGCTAGTGAGGGGTATTC+CGG 0.301510 tig0020315:+144200 Msa1380010:CDS
GGCGCTAGGGTTGGGGTTGA+AGG 0.302792 tig0020315:-141545 None:intergenic
GGTTTCCTCCTTCTCTTTGC+GGG 0.349900 tig0020315:-144460 None:intergenic
TCTTCAAATGCCTAAGTTTG+AGG 0.363190 tig0020315:+144584 Msa1380010:three_prime_UTR
ACAGTTTATCAATTTCAGTT+TGG 0.366118 tig0020315:+144724 Msa1380010:three_prime_UTR
TGCGGTGGTTCCGTCAAAAC+GGG 0.378616 tig0020315:-141594 None:intergenic
GTTTGAGGTTGTGCCTCATA+TGG 0.383938 tig0020315:+144599 Msa1380010:three_prime_UTR
TCAACCTGCTTCTTCTGCTT+AGG 0.384301 tig0020315:+144518 Msa1380010:CDS
CAACCTGCTTCTTCTGCTTA+GGG 0.390606 tig0020315:+144519 Msa1380010:CDS
CGCTAGGGTTGGGGTTGAAG+GGG 0.397527 tig0020315:-141543 None:intergenic
GATGGAACACACCTACTGTT+AGG 0.400025 tig0020315:+144292 Msa1380010:CDS
CACTAGCGTTATGTAGAGAT+TGG 0.401453 tig0020315:-144187 None:intergenic
GAGGCCGGCGGCGCTAGGGT+TGG 0.409277 tig0020315:-141554 None:intergenic
ACGCTAGTGAGGGGTATTCC+GGG 0.415196 tig0020315:+144201 Msa1380010:CDS
GGGTTTCCTCCTTCTCTTTG+CGG 0.418532 tig0020315:-144461 None:intergenic
AGATTGGGGCTATAAGCAAT+TGG 0.427253 tig0020315:-144171 None:intergenic
CATGAGCATGAATGCGTTCA+AGG 0.431873 tig0020315:+144131 Msa1380010:exon
CTTTCAACCAACTCCACTCC+GGG 0.433958 tig0020315:-141492 None:intergenic
AAGCAGAAGAAGCAGGTTGA+TGG 0.435151 tig0020315:-144515 None:intergenic
GGCCGGCGGCGCTAGGGTTG+GGG 0.438610 tig0020315:-141552 None:intergenic
AAGTAGAGGCCGGCGGCGCT+AGG 0.459350 tig0020315:-141559 None:intergenic
ACTAGCGTTATGTAGAGATT+GGG 0.460548 tig0020315:-144186 None:intergenic
ATGGAACACACCTACTGTTA+GGG 0.464145 tig0020315:+144293 Msa1380010:CDS
AGAGAAGAGACGTAAAATAG+AGG 0.464708 tig0020315:+141459 Msa1380010:five_prime_UTR
GTTAGTTGTTGTTGAGACAG+AGG 0.465463 tig0020315:+144425 Msa1380010:CDS
TGTACGAATAGACTGAATTT+CGG 0.468402 tig0020315:+141417 Msa1380010:five_prime_UTR
ATGCGGTGGTTCCGTCAAAA+CGG 0.475445 tig0020315:-141595 None:intergenic
GAGAAGAGACGTAAAATAGA+GGG 0.475475 tig0020315:+141460 Msa1380010:five_prime_UTR
AAAGCAAATAAAACCATATG+AGG 0.477102 tig0020315:-144612 None:intergenic
CTATGTTGCTTTCTGGTCCC+CGG 0.490539 tig0020315:-144219 None:intergenic
GCGCTAGGGTTGGGGTTGAA+GGG 0.493852 tig0020315:-141544 None:intergenic
CAGAGCTCTTCGTCCTCCAT+TGG 0.509479 tig0020315:+144485 Msa1380010:CDS
TGTTCCATCGCATGACAGTT+CGG 0.511917 tig0020315:-144278 None:intergenic
AGTAGAGGCCGGCGGCGCTA+GGG 0.513498 tig0020315:-141558 None:intergenic
TGGTTCCGTCAAAACGGGGA+AGG 0.514256 tig0020315:-141589 None:intergenic
GCTTTCAACCAACTCCACTC+CGG 0.516574 tig0020315:-141493 None:intergenic
AACCCCAACCCTAGCGCCGC+CGG 0.521091 tig0020315:+141550 Msa1380010:five_prime_UTR
GGTTGATGGTTTATGACCAA+TGG 0.522943 tig0020315:-144501 None:intergenic
GAAGTGACCCGGAGTGGAGT+TGG 0.525746 tig0020315:+141485 Msa1380010:five_prime_UTR
CTCTAAGTACAACTTAGATT+AGG 0.526741 tig0020315:-141390 None:intergenic
GATTGGGGCTATAAGCAATT+GGG 0.527244 tig0020315:-144170 None:intergenic
TGTATTTGATATAGGTCAAG+AGG 0.544788 tig0020315:+144403 Msa1380010:intron
GGTTCCGTCAAAACGGGGAA+GGG 0.549299 tig0020315:-141588 None:intergenic
GTACCCTAAGCAGAAGAAGC+AGG 0.555500 tig0020315:-144522 None:intergenic
GTACAAGGCCCGCAAAGAGA+AGG 0.555978 tig0020315:+144452 Msa1380010:CDS
AAAGCAACATAGGTGCACTT+TGG 0.556523 tig0020315:+144230 Msa1380010:CDS
CGTCAAAACGGGGAAGGGCA+AGG 0.564587 tig0020315:-141583 None:intergenic
ATAGACTGAATTTCGGAGAA+GGG 0.567788 tig0020315:+141424 Msa1380010:five_prime_UTR
TGGAGGACGAAGAGCTCTGT+GGG 0.570519 tig0020315:-144481 None:intergenic
ATCTAAGTTGTACTTAGAGA+TGG 0.572654 tig0020315:+141394 Msa1380010:five_prime_UTR
AGAAGAGACGTAAAATAGAG+GGG 0.574408 tig0020315:+141461 Msa1380010:five_prime_UTR
AGGGCAAGGAAAGTAGAGGC+CGG 0.576902 tig0020315:-141569 None:intergenic
AATAGAGGGGAGAAGTGACC+CGG 0.577040 tig0020315:+141474 Msa1380010:five_prime_UTR
AATAGACTGAATTTCGGAGA+AGG 0.579412 tig0020315:+141423 Msa1380010:five_prime_UTR
CGGGGACCAGAAAGCAACAT+AGG 0.579617 tig0020315:+144220 Msa1380010:CDS
AGGGGAACGCTGATTTGAGA+AGG 0.583542 tig0020315:-141525 None:intergenic
TGTTAGGGGAATGATCCAGC+AGG 0.585091 tig0020315:+144308 Msa1380010:CDS
TGAGGCACAACCTCAAACTT+AGG 0.586101 tig0020315:-144594 None:intergenic
GCTATAAGCAATTGGGACAC+AGG 0.590348 tig0020315:-144163 None:intergenic
TGACGGAACCACCGCATAGG+AGG 0.593538 tig0020315:+141601 Msa1380010:five_prime_UTR
CCATATCAGCAAACATTGCA+AGG 0.597443 tig0020315:-144544 None:intergenic
GCAACCGAACTGTCATGCGA+TGG 0.602415 tig0020315:+144274 Msa1380010:CDS
TAAACTGTTTCAAGCACAGA+GGG 0.603667 tig0020315:-144709 None:intergenic
CAAGGCCCGCAAAGAGAAGG+AGG 0.604236 tig0020315:+144455 Msa1380010:CDS
ATCTCTACATAACGCTAGTG+AGG 0.609634 tig0020315:+144190 Msa1380010:CDS
CTAGCGTTATGTAGAGATTG+GGG 0.616077 tig0020315:-144185 None:intergenic
TGGATCATTCCCCTAACAGT+AGG 0.620579 tig0020315:-144303 None:intergenic
TGATGGTTTATGACCAATGG+AGG 0.622285 tig0020315:-144498 None:intergenic
CAGTTCGGTTGCATTTGCCG+AGG 0.626107 tig0020315:-144263 None:intergenic
TCTCTACATAACGCTAGTGA+GGG 0.627577 tig0020315:+144191 Msa1380010:CDS
AGGGGAGAAGTGACCCGGAG+TGG 0.637707 tig0020315:+141479 Msa1380010:five_prime_UTR
GGAACGCTGATTTGAGAAGG+AGG 0.641765 tig0020315:-141522 None:intergenic
TGAGACAGAGGAGATGTACA+AGG 0.642473 tig0020315:+144437 Msa1380010:CDS
GCAAGGAAAGTAGAGGCCGG+CGG 0.651496 tig0020315:-141566 None:intergenic
TGGAACACACCTACTGTTAG+GGG 0.654422 tig0020315:+144294 Msa1380010:CDS
GGGAAGGGCAAGGAAAGTAG+AGG 0.662057 tig0020315:-141573 None:intergenic
ATGGAGGACGAAGAGCTCTG+TGG 0.663844 tig0020315:-144482 None:intergenic
CGCTAGTGAGGGGTATTCCG+GGG 0.667158 tig0020315:+144202 Msa1380010:CDS
AAGACTTACCTCCTATGCGG+TGG 0.667701 tig0020315:-141609 None:intergenic
CTCTACATAACGCTAGTGAG+GGG 0.670443 tig0020315:+144192 Msa1380010:CDS
ACTTTGGAAGCACTACGCCT+CGG 0.674356 tig0020315:+144246 Msa1380010:CDS
ATAAACTGTTTCAAGCACAG+AGG 0.678073 tig0020315:-144710 None:intergenic
GCGGTGGTTCCGTCAAAACG+GGG 0.711779 tig0020315:-141593 None:intergenic
TCAAAGACTTACCTCCTATG+CGG 0.743924 tig0020315:-141612 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAATGATTGATATAATA+TGG + tig0020315:142750-142769 Msa1380010:intron 10.0%
!! AAAATTTAAATAAAATTGGA+AGG - tig0020315:143999-144018 None:intergenic 10.0%
!!! TTATGCTTTTATTAATTCAA+GGG + tig0020315:141711-141730 Msa1380010:intron 15.0%
!!! TTTTTTTTTTTTACTATAGC+TGG + tig0020315:141955-141974 Msa1380010:intron 15.0%
!! AAATAAAAAAATAAAGAACG+TGG - tig0020315:143233-143252 None:intergenic 15.0%
!!! TTTTTTAGTAATAAGTCATA+AGG + tig0020315:143409-143428 Msa1380010:intron 15.0%
!!! GTTATGCTTTTATTAATTCA+AGG + tig0020315:141710-141729 Msa1380010:intron 20.0%
!! TTTATTATGAAGCATGAATT+GGG - tig0020315:142313-142332 None:intergenic 20.0%
!!! TTTTATTATGAAGCATGAAT+TGG - tig0020315:142314-142333 None:intergenic 20.0%
!!! AAATAAACAACTCCATTTTA+TGG - tig0020315:142972-142991 None:intergenic 20.0%
!!! TAAAATGGAGTTGTTTATTT+TGG + tig0020315:142972-142991 Msa1380010:intron 20.0%
!!! ATTTTTATTCCCATGAAATA+GGG + tig0020315:143176-143195 Msa1380010:intron 20.0%
!! AAAAAAAAAAATCAAGAACG+TGG - tig0020315:143296-143315 None:intergenic 20.0%
!!! TATGTTAATTTTTACAACCT+GGG - tig0020315:143378-143397 None:intergenic 20.0%
!!! TTATGTTAATTTTTACAACC+TGG - tig0020315:143379-143398 None:intergenic 20.0%
!!! GAATTTTTAAGAAAAGTGTT+TGG - tig0020315:143492-143511 None:intergenic 20.0%
!!! ATAATGAGAATTTTATTCCT+AGG - tig0020315:143514-143533 None:intergenic 20.0%
! TTTCATACTTGATTGATTGA+TGG + tig0020315:141660-141679 Msa1380010:intron 25.0%
! CTAAATGATTAATCTCATAC+TGG - tig0020315:141786-141805 None:intergenic 25.0%
!! CATTTTCTTAAATTACTGTC+AGG - tig0020315:141835-141854 None:intergenic 25.0%
! TAATGAAGAAAACAAAAGCA+AGG - tig0020315:142066-142085 None:intergenic 25.0%
!! TTTAACATAACATCGATTGA+TGG + tig0020315:142268-142287 Msa1380010:intron 25.0%
! TAGTATATACTGAAACTGAA+AGG - tig0020315:142341-142360 None:intergenic 25.0%
! TATATACTATCGTTGAATAG+CGG + tig0020315:142352-142371 Msa1380010:intron 25.0%
!!! GTATTTTTTGAAATGTCATG+AGG + tig0020315:142835-142854 Msa1380010:intron 25.0%
! TCTTAATATAACAATCCGAT+CGG + tig0020315:142876-142895 Msa1380010:intron 25.0%
! ATAAAAATGACGAGAATGTA+AGG - tig0020315:143164-143183 None:intergenic 25.0%
!! CATTTTTATTCCCATGAAAT+AGG + tig0020315:143175-143194 Msa1380010:intron 25.0%
!! CACTTTTCTTAAAAATTCCT+AGG + tig0020315:143494-143513 Msa1380010:intron 25.0%
!! AAATTCTCATTATGTTTTCC+TGG + tig0020315:143521-143540 Msa1380010:intron 25.0%
!! AATTCTCATTATGTTTTCCT+GGG + tig0020315:143522-143541 Msa1380010:intron 25.0%
! GAACTAAAAAATTGTATTGG+AGG + tig0020315:143742-143761 Msa1380010:intron 25.0%
! TAAAAAATTGTATTGGAGGA+GGG + tig0020315:143746-143765 Msa1380010:intron 25.0%
! GGTAACTTTGTATTTGATAT+AGG + tig0020315:144395-144414 Msa1380010:intron 25.0%
! AAAGCAAATAAAACCATATG+AGG - tig0020315:144615-144634 None:intergenic 25.0%
! ACAGTTTATCAATTTCAGTT+TGG + tig0020315:144724-144743 Msa1380010:three_prime_UTR 25.0%
CTCTAAGTACAACTTAGATT+AGG - tig0020315:141393-141412 None:intergenic 30.0%
ATCTAAGTTGTACTTAGAGA+TGG + tig0020315:141394-141413 Msa1380010:five_prime_UTR 30.0%
TGTACGAATAGACTGAATTT+CGG + tig0020315:141417-141436 Msa1380010:five_prime_UTR 30.0%
AACAACCTAAACCTTAACAT+TGG - tig0020315:141688-141707 None:intergenic 30.0%
!! ATGTTAAGGTTTAGGTTGTT+TGG + tig0020315:141688-141707 Msa1380010:intron 30.0%
! TGCTTTTATTAATTCAAGGG+AGG + tig0020315:141714-141733 Msa1380010:intron 30.0%
! CTAATCATATGTGTTGATGT+TGG + tig0020315:141867-141886 Msa1380010:intron 30.0%
!!! AATTTGCTGCCTATGTTTTA+TGG - tig0020315:141922-141941 None:intergenic 30.0%
TCTTCATTAAAACTTCAGCA+AGG + tig0020315:142077-142096 Msa1380010:intron 30.0%
!! GCTTTTGTTGTGCTATATTT+CGG + tig0020315:142205-142224 Msa1380010:intron 30.0%
!! TTGTTTTTCAGTTAGTTGTC+AGG + tig0020315:142562-142581 Msa1380010:intron 30.0%
! ATGCATCAGTTGTCAATTTT+AGG + tig0020315:142616-142635 Msa1380010:intron 30.0%
! AGTACAACTTTTAGTATGGA+AGG + tig0020315:142642-142661 Msa1380010:intron 30.0%
! TGAACCAATTTAAAACCGAT+CGG - tig0020315:142894-142913 None:intergenic 30.0%
AAAACTCAAAACTAGTGTGA+GGG + tig0020315:142915-142934 Msa1380010:intron 30.0%
AGAGATAAAAAACACATTCC+TGG + tig0020315:143046-143065 Msa1380010:intron 30.0%
GAGATAAAAAACACATTCCT+GGG + tig0020315:143047-143066 Msa1380010:intron 30.0%
GAAAGAAGAATACCTATGTT+TGG + tig0020315:143082-143101 Msa1380010:intron 30.0%
AATACCTATGTTTGGTAGAA+AGG + tig0020315:143090-143109 Msa1380010:intron 30.0%
GGAACAAAAATAATATGCCT+AGG + tig0020315:143111-143130 Msa1380010:intron 30.0%
AAAAAAATCAAGAACGTGGT+TGG - tig0020315:143292-143311 None:intergenic 30.0%
! ATGAGTTTTTATCAAGAACG+TGG - tig0020315:143330-143349 None:intergenic 30.0%
TGGGAACACAATAGTAAAAT+TGG - tig0020315:143359-143378 None:intergenic 30.0%
! AATTTTACTATTGTGTTCCC+AGG + tig0020315:143358-143377 Msa1380010:intron 30.0%
! AGGATTCTTTTCAAAGAAGT+GGG - tig0020315:143451-143470 None:intergenic 30.0%
! TAGGATTCTTTTCAAAGAAG+TGG - tig0020315:143452-143471 None:intergenic 30.0%
! ACTTCTTTGAAAAGAATCCT+AGG + tig0020315:143451-143470 Msa1380010:intron 30.0%
! GGTTGTCAATTTTTATTCCT+AGG - tig0020315:143471-143490 None:intergenic 30.0%
TGGAAAAAATAGAGGACATT+GGG + tig0020315:143660-143679 Msa1380010:intron 30.0%
!!! ATTTTTTAGTTCCCCCAAAT+GGG - tig0020315:143734-143753 None:intergenic 30.0%
!!! AATTTTTTAGTTCCCCCAAA+TGG - tig0020315:143735-143754 None:intergenic 30.0%
GGGGAACTAAAAAATTGTAT+TGG + tig0020315:143739-143758 Msa1380010:intron 30.0%
CTAAAAAATTGTATTGGAGG+AGG + tig0020315:143745-143764 Msa1380010:intron 30.0%
AAATTGGAAGGAAAACACTT+TGG - tig0020315:143987-144006 None:intergenic 30.0%
ACCAATGTTGTTAGTATACA+GGG - tig0020315:144378-144397 None:intergenic 30.0%
TACCAATGTTGTTAGTATAC+AGG - tig0020315:144379-144398 None:intergenic 30.0%
TGTATTTGATATAGGTCAAG+AGG + tig0020315:144403-144422 Msa1380010:intron 30.0%
! ACTTAGGCATTTGAAGATTT+TGG - tig0020315:144581-144600 None:intergenic 30.0%
AATAGACTGAATTTCGGAGA+AGG + tig0020315:141423-141442 Msa1380010:five_prime_UTR 35.0%
ATAGACTGAATTTCGGAGAA+GGG + tig0020315:141424-141443 Msa1380010:five_prime_UTR 35.0%
AGAGAAGAGACGTAAAATAG+AGG + tig0020315:141459-141478 Msa1380010:five_prime_UTR 35.0%
GAGAAGAGACGTAAAATAGA+GGG + tig0020315:141460-141479 Msa1380010:five_prime_UTR 35.0%
AGAAGAGACGTAAAATAGAG+GGG + tig0020315:141461-141480 Msa1380010:five_prime_UTR 35.0%
! GATTGATGGATCCAATGTTA+AGG + tig0020315:141674-141693 Msa1380010:intron 35.0%
TGGATCCAATGTTAAGGTTT+AGG + tig0020315:141680-141699 Msa1380010:intron 35.0%
TTATGGACACTTCTGATTGA+AGG - tig0020315:141905-141924 None:intergenic 35.0%
!! CAGAAGTGTCCATAAAACAT+AGG + tig0020315:141910-141929 Msa1380010:intron 35.0%
CCAATTTAAGCATATGTTGC+AGG - tig0020315:142152-142171 None:intergenic 35.0%
CCTGCAACATATGCTTAAAT+TGG + tig0020315:142149-142168 Msa1380010:intron 35.0%
!! CTCTGTCATCTTTTGTTTAG+TGG + tig0020315:142183-142202 Msa1380010:intron 35.0%
! GTGCAGTACAACTTTTAGTA+TGG + tig0020315:142638-142657 Msa1380010:intron 35.0%
!!! CAATCCGATCGGTTTTAAAT+TGG + tig0020315:142887-142906 Msa1380010:intron 35.0%
CAAAACTCAAAACTAGTGTG+AGG + tig0020315:142914-142933 Msa1380010:intron 35.0%
AAACTCAAAACTAGTGTGAG+GGG + tig0020315:142916-142935 Msa1380010:intron 35.0%
! AACAACTCCATTTTATGGTC+CGG - tig0020315:142967-142986 None:intergenic 35.0%
TTGGTTCAGTTAATTGAACC+AGG + tig0020315:142991-143010 Msa1380010:intron 35.0%
TGTTCCTTTCTACCAAACAT+AGG - tig0020315:143097-143116 None:intergenic 35.0%
TGTAAGGTAAGTAGATTCCT+GGG - tig0020315:143148-143167 None:intergenic 35.0%
ATGTAAGGTAAGTAGATTCC+TGG - tig0020315:143149-143168 None:intergenic 35.0%
TTATTCCCATGAAATAGGGA+TGG + tig0020315:143180-143199 Msa1380010:intron 35.0%
TATTCCCATGAAATAGGGAT+GGG + tig0020315:143181-143200 Msa1380010:intron 35.0%
! TTTTCCCATCCCTATTTCAT+GGG - tig0020315:143188-143207 None:intergenic 35.0%
! ATTTTCCCATCCCTATTTCA+TGG - tig0020315:143189-143208 None:intergenic 35.0%
GATGGGAAAATTATATTCCC+AGG + tig0020315:143198-143217 Msa1380010:intron 35.0%
! GTTTTTATCAAGAACGTGGT+TGG - tig0020315:143326-143345 None:intergenic 35.0%
! ATTCTTTTCAAAGAAGTGGG+TGG - tig0020315:143448-143467 None:intergenic 35.0%
GAAAAAGATGAAGGAGTAAG+TGG + tig0020315:143640-143659 Msa1380010:intron 35.0%
GGAGTAAGTGGAAAAAATAG+AGG + tig0020315:143652-143671 Msa1380010:intron 35.0%
GTGGAAAAAATAGAGGACAT+TGG + tig0020315:143659-143678 Msa1380010:intron 35.0%
AAAAAATAGAGGACATTGGG+AGG + tig0020315:143663-143682 Msa1380010:intron 35.0%
AATATAAAACCCTCCCCATT+TGG + tig0020315:143717-143736 Msa1380010:intron 35.0%
ATATAAAACCCTCCCCATTT+GGG + tig0020315:143718-143737 Msa1380010:intron 35.0%
!!! TTTTTTAGTTCCCCCAAATG+GGG - tig0020315:143733-143752 None:intergenic 35.0%
ACTAGCGTTATGTAGAGATT+GGG - tig0020315:144189-144208 None:intergenic 35.0%
GGAGATAGGTAGTTTCAAAA+TGG - tig0020315:144357-144376 None:intergenic 35.0%
TTGTTAGTATACAGGGAGAT+AGG - tig0020315:144371-144390 None:intergenic 35.0%
TCCCTGTATACTAACAACAT+TGG + tig0020315:144374-144393 Msa1380010:intron 35.0%
TCTTCAAATGCCTAAGTTTG+AGG + tig0020315:144584-144603 Msa1380010:three_prime_UTR 35.0%
TAAACTGTTTCAAGCACAGA+GGG - tig0020315:144712-144731 None:intergenic 35.0%
ATAAACTGTTTCAAGCACAG+AGG - tig0020315:144713-144732 None:intergenic 35.0%
TCAAAGACTTACCTCCTATG+CGG - tig0020315:141615-141634 None:intergenic 40.0%
! TATGTGTTGATGTTGGACAC+CGG + tig0020315:141874-141893 Msa1380010:intron 40.0%
TTGTGAAGTAGCTGCTATAG+CGG + tig0020315:142444-142463 Msa1380010:intron 40.0%
TCGCATATTGCAGAAAAGAG+CGG - tig0020315:142506-142525 None:intergenic 40.0%
! GAAGGCATAACTGTGATGTT+TGG + tig0020315:142660-142679 Msa1380010:intron 40.0%
! GAAAGCATAACCGTGATGTT+TGG + tig0020315:142782-142801 Msa1380010:intron 40.0%
TACAGTGACACCAAACATCA+CGG - tig0020315:142795-142814 None:intergenic 40.0%
AGGCATGAACCAATCTCTAA+GGG + tig0020315:143011-143030 Msa1380010:intron 40.0%
AAACACATTCCTGGGAATAG+AGG + tig0020315:143055-143074 Msa1380010:intron 40.0%
CCTGGGCATTAATTATTCCT+AGG - tig0020315:143131-143150 None:intergenic 40.0%
CCTAGGAATAATTAATGCCC+AGG + tig0020315:143128-143147 Msa1380010:intron 40.0%
TTCCTGGGAAAGTTAATTCC+AGG + tig0020315:143537-143556 Msa1380010:intron 40.0%
TCCTGGGAAAGTTAATTCCA+GGG + tig0020315:143538-143557 Msa1380010:intron 40.0%
! CAGGGAGTAATTTTTGCATC+CGG + tig0020315:143556-143575 Msa1380010:intron 40.0%
! AGGGAGTAATTTTTGCATCC+GGG + tig0020315:143557-143576 Msa1380010:intron 40.0%
AACTCGCAAAGAAAGCCTTA+GGG - tig0020315:143594-143613 None:intergenic 40.0%
TAAGGCTTTCTTTGCGAGTT+CGG + tig0020315:143594-143613 Msa1380010:intron 40.0%
GAGGGTTTGGAAAAAGATGA+AGG + tig0020315:143631-143650 Msa1380010:intron 40.0%
TATAAAACCCTCCCCATTTG+GGG + tig0020315:143719-143738 Msa1380010:intron 40.0%
! ATTGTATTGGAGGAGGGTTT+TGG + tig0020315:143752-143771 Msa1380010:intron 40.0%
! TTGTATTGGAGGAGGGTTTT+GGG + tig0020315:143753-143772 Msa1380010:intron 40.0%
TCTGTGGGGTGAGAAATATA+TGG - tig0020315:143926-143945 None:intergenic 40.0%
TTTGGCATTGTTTGCGAGTT+TGG - tig0020315:143969-143988 None:intergenic 40.0%
CTGTTCTGACTTCAACTTGT+AGG + tig0020315:144108-144127 Msa1380010:intron 40.0%
GATTGGGGCTATAAGCAATT+GGG - tig0020315:144173-144192 None:intergenic 40.0%
AGATTGGGGCTATAAGCAAT+TGG - tig0020315:144174-144193 None:intergenic 40.0%
CTAGCGTTATGTAGAGATTG+GGG - tig0020315:144188-144207 None:intergenic 40.0%
CACTAGCGTTATGTAGAGAT+TGG - tig0020315:144190-144209 None:intergenic 40.0%
ATCTCTACATAACGCTAGTG+AGG + tig0020315:144190-144209 Msa1380010:CDS 40.0%
TCTCTACATAACGCTAGTGA+GGG + tig0020315:144191-144210 Msa1380010:CDS 40.0%
AAAGCAACATAGGTGCACTT+TGG + tig0020315:144230-144249 Msa1380010:CDS 40.0%
ATGGAACACACCTACTGTTA+GGG + tig0020315:144293-144312 Msa1380010:CDS 40.0%
! GTTAGTTGTTGTTGAGACAG+AGG + tig0020315:144425-144444 Msa1380010:CDS 40.0%
TGATGGTTTATGACCAATGG+AGG - tig0020315:144501-144520 None:intergenic 40.0%
!! GGTTGATGGTTTATGACCAA+TGG - tig0020315:144504-144523 None:intergenic 40.0%
CCATATCAGCAAACATTGCA+AGG - tig0020315:144547-144566 None:intergenic 40.0%
! CCTTGCAATGTTTGCTGATA+TGG + tig0020315:144544-144563 Msa1380010:three_prime_UTR 40.0%
TGTGCTATATTTCGGCAAGC+AGG + tig0020315:142213-142232 Msa1380010:intron 45.0%
! CTTAGAGATTGGTTCATGCC+TGG - tig0020315:143012-143031 None:intergenic 45.0%
CAGGCATGAACCAATCTCTA+AGG + tig0020315:143010-143029 Msa1380010:intron 45.0%
GGCATGAACCAATCTCTAAG+GGG + tig0020315:143012-143031 Msa1380010:intron 45.0%
! GCATGAACCAATCTCTAAGG+GGG + tig0020315:143013-143032 Msa1380010:intron 45.0%
ACATTCCTGGGAATAGAGGT+TGG + tig0020315:143059-143078 Msa1380010:intron 45.0%
CATTCCTGGGAATAGAGGTT+GGG + tig0020315:143060-143079 Msa1380010:intron 45.0%
TCCCTGGAATTAACTTTCCC+AGG - tig0020315:143542-143561 None:intergenic 45.0%
CGGATGCAAAAATTACTCCC+TGG - tig0020315:143558-143577 None:intergenic 45.0%
ACTCGCAAAGAAAGCCTTAG+GGG - tig0020315:143593-143612 None:intergenic 45.0%
GAACTCGCAAAGAAAGCCTT+AGG - tig0020315:143595-143614 None:intergenic 45.0%
ATAAAACCCTCCCCATTTGG+GGG + tig0020315:143720-143739 Msa1380010:intron 45.0%
! TGTATTGGAGGAGGGTTTTG+GGG + tig0020315:143754-143773 Msa1380010:intron 45.0%
CATTGTTTGCGAGTTTGGAG+GGG - tig0020315:143964-143983 None:intergenic 45.0%
GCATTGTTTGCGAGTTTGGA+GGG - tig0020315:143965-143984 None:intergenic 45.0%
CATGAGCATGAATGCGTTCA+AGG + tig0020315:144131-144150 Msa1380010:exon 45.0%
GCTATAAGCAATTGGGACAC+AGG - tig0020315:144166-144185 None:intergenic 45.0%
CTCTACATAACGCTAGTGAG+GGG + tig0020315:144192-144211 Msa1380010:CDS 45.0%
!! AGTGCACCTATGTTGCTTTC+TGG - tig0020315:144229-144248 None:intergenic 45.0%
TGTTCCATCGCATGACAGTT+CGG - tig0020315:144281-144300 None:intergenic 45.0%
GATGGAACACACCTACTGTT+AGG + tig0020315:144292-144311 Msa1380010:CDS 45.0%
TGGAACACACCTACTGTTAG+GGG + tig0020315:144294-144313 Msa1380010:CDS 45.0%
TGGATCATTCCCCTAACAGT+AGG - tig0020315:144306-144325 None:intergenic 45.0%
TGAGACAGAGGAGATGTACA+AGG + tig0020315:144437-144456 Msa1380010:CDS 45.0%
! AAGCAGAAGAAGCAGGTTGA+TGG - tig0020315:144518-144537 None:intergenic 45.0%
TCAACCTGCTTCTTCTGCTT+AGG + tig0020315:144518-144537 Msa1380010:CDS 45.0%
CAACCTGCTTCTTCTGCTTA+GGG + tig0020315:144519-144538 Msa1380010:CDS 45.0%
TGAGGCACAACCTCAAACTT+AGG - tig0020315:144597-144616 None:intergenic 45.0%
! GTTTGAGGTTGTGCCTCATA+TGG + tig0020315:144599-144618 Msa1380010:three_prime_UTR 45.0%
!! CAATATATTATATATAAATA+TGG + tig0020315:142530-142549 Msa1380010:intron 5.0%
!! AGATAAAATTTAAATAAAAT+TGG - tig0020315:144003-144022 None:intergenic 5.0%
AATAGAGGGGAGAAGTGACC+CGG + tig0020315:141474-141493 Msa1380010:five_prime_UTR 50.0%
CTTTCAACCAACTCCACTCC+GGG - tig0020315:141495-141514 None:intergenic 50.0%
GCTTTCAACCAACTCCACTC+CGG - tig0020315:141496-141515 None:intergenic 50.0%
!! GGAACGCTGATTTGAGAAGG+AGG - tig0020315:141525-141544 None:intergenic 50.0%
!! AGGGGAACGCTGATTTGAGA+AGG - tig0020315:141528-141547 None:intergenic 50.0%
ATGCGGTGGTTCCGTCAAAA+CGG - tig0020315:141598-141617 None:intergenic 50.0%
! TTTTGACGGAACCACCGCAT+AGG + tig0020315:141598-141617 Msa1380010:five_prime_UTR 50.0%
AAGACTTACCTCCTATGCGG+TGG - tig0020315:141612-141631 None:intergenic 50.0%
! CTTCTGATTGAAGGCGTGTC+CGG - tig0020315:141896-141915 None:intergenic 50.0%
CTCAAAACTAGTGTGAGGGG+AGG + tig0020315:142919-142938 Msa1380010:intron 50.0%
CTAGTGTGAGGGGAGGAAAA+TGG + tig0020315:142926-142945 Msa1380010:intron 50.0%
ATGGCTCAGTTTGCGAAAGC+CGG + tig0020315:142945-142964 Msa1380010:intron 50.0%
GCGAAAGCCGGACCATAAAA+TGG + tig0020315:142957-142976 Msa1380010:intron 50.0%
ACAAACGCCCCCTTAGAGAT+TGG - tig0020315:143023-143042 None:intergenic 50.0%
CTTTCCCAACCTCTATTCCC+AGG - tig0020315:143067-143086 None:intergenic 50.0%
! GAACGTGGCACTCTTTTCCT+GGG - tig0020315:143218-143237 None:intergenic 50.0%
! AGAACGTGGCACTCTTTTCC+TGG - tig0020315:143219-143238 None:intergenic 50.0%
!! CTTAGGGGGTGTTTGTTTCC+CGG - tig0020315:143578-143597 None:intergenic 50.0%
CTCGCAAAGAAAGCCTTAGG+GGG - tig0020315:143592-143611 None:intergenic 50.0%
GCTTTCTTTGCGAGTTCGGA+GGG + tig0020315:143598-143617 Msa1380010:intron 50.0%
CTTTCTTTGCGAGTTCGGAG+GGG + tig0020315:143599-143618 Msa1380010:intron 50.0%
ATAGAGGACATTGGGAGGAG+AGG + tig0020315:143668-143687 Msa1380010:intron 50.0%
TAGAGGACATTGGGAGGAGA+GGG + tig0020315:143669-143688 Msa1380010:intron 50.0%
TTAGTTCCCCCAAATGGGGA+GGG - tig0020315:143729-143748 None:intergenic 50.0%
! TTTAGTTCCCCCAAATGGGG+AGG - tig0020315:143730-143749 None:intergenic 50.0%
!! GTATTGGAGGAGGGTTTTGG+GGG + tig0020315:143755-143774 Msa1380010:intron 50.0%
!!! TATTGGAGGAGGGTTTTGGG+GGG + tig0020315:143756-143775 Msa1380010:intron 50.0%
TTTGCGAGTTTGGAGGGGAA+GGG - tig0020315:143959-143978 None:intergenic 50.0%
GGCATTGTTTGCGAGTTTGG+AGG - tig0020315:143966-143985 None:intergenic 50.0%
!! AACGCTAGTGAGGGGTATTC+CGG + tig0020315:144200-144219 Msa1380010:CDS 50.0%
!! CTATGTTGCTTTCTGGTCCC+CGG - tig0020315:144222-144241 None:intergenic 50.0%
!! ACTTTGGAAGCACTACGCCT+CGG + tig0020315:144246-144265 Msa1380010:CDS 50.0%
TGTTAGGGGAATGATCCAGC+AGG + tig0020315:144308-144327 Msa1380010:CDS 50.0%
GATCTTGTGCTGAAACCTGC+TGG - tig0020315:144326-144345 None:intergenic 50.0%
GGTTTCCTCCTTCTCTTTGC+GGG - tig0020315:144463-144482 None:intergenic 50.0%
GGGTTTCCTCCTTCTCTTTG+CGG - tig0020315:144464-144483 None:intergenic 50.0%
GTACCCTAAGCAGAAGAAGC+AGG - tig0020315:144525-144544 None:intergenic 50.0%
AGGGCAAGGAAAGTAGAGGC+CGG - tig0020315:141572-141591 None:intergenic 55.0%
GGGAAGGGCAAGGAAAGTAG+AGG - tig0020315:141576-141595 None:intergenic 55.0%
! CTTGCCCTTCCCCGTTTTGA+CGG + tig0020315:141584-141603 Msa1380010:five_prime_UTR 55.0%
GGTTCCGTCAAAACGGGGAA+GGG - tig0020315:141591-141610 None:intergenic 55.0%
TGGTTCCGTCAAAACGGGGA+AGG - tig0020315:141592-141611 None:intergenic 55.0%
TGCGGTGGTTCCGTCAAAAC+GGG - tig0020315:141597-141616 None:intergenic 55.0%
!! GGTGTCGACGTGTAAGTGTG+AGG - tig0020315:141814-141833 None:intergenic 55.0%
GCAAGCAGGTCTGAATCTGC+TGG + tig0020315:142227-142246 Msa1380010:intron 55.0%
CGGGAAACAAACACCCCCTA+AGG + tig0020315:143576-143595 Msa1380010:intron 55.0%
GGCTTTCTTTGCGAGTTCGG+AGG + tig0020315:143597-143616 Msa1380010:intron 55.0%
TTTGCGAGTTCGGAGGGGAA+GGG + tig0020315:143604-143623 Msa1380010:intron 55.0%
! TTGGGAGGAGAGGGCTTTGA+CGG + tig0020315:143678-143697 Msa1380010:intron 55.0%
!!! AGGAGGGTTTTGGGGGGTTT+GGG + tig0020315:143762-143781 Msa1380010:intron 55.0%
AAGGGAAGGAAGGGCTCTGT+GGG - tig0020315:143941-143960 None:intergenic 55.0%
TTGGAGGGGAAGGGAAGGAA+GGG - tig0020315:143950-143969 None:intergenic 55.0%
TTTGGAGGGGAAGGGAAGGA+AGG - tig0020315:143951-143970 None:intergenic 55.0%
GTTTGCGAGTTTGGAGGGGA+AGG - tig0020315:143960-143979 None:intergenic 55.0%
!! ACGCTAGTGAGGGGTATTCC+GGG + tig0020315:144201-144220 Msa1380010:CDS 55.0%
CGGGGACCAGAAAGCAACAT+AGG + tig0020315:144220-144239 Msa1380010:CDS 55.0%
CAGTTCGGTTGCATTTGCCG+AGG - tig0020315:144266-144285 None:intergenic 55.0%
GCAACCGAACTGTCATGCGA+TGG + tig0020315:144274-144293 Msa1380010:CDS 55.0%
GTACAAGGCCCGCAAAGAGA+AGG + tig0020315:144452-144471 Msa1380010:CDS 55.0%
TGGAGGACGAAGAGCTCTGT+GGG - tig0020315:144484-144503 None:intergenic 55.0%
ATGGAGGACGAAGAGCTCTG+TGG - tig0020315:144485-144504 None:intergenic 55.0%
CAGAGCTCTTCGTCCTCCAT+TGG + tig0020315:144485-144504 Msa1380010:CDS 55.0%
GAAGTGACCCGGAGTGGAGT+TGG + tig0020315:141485-141504 Msa1380010:five_prime_UTR 60.0%
CGCTAGGGTTGGGGTTGAAG+GGG - tig0020315:141546-141565 None:intergenic 60.0%
GCGCTAGGGTTGGGGTTGAA+GGG - tig0020315:141547-141566 None:intergenic 60.0%
GCAAGGAAAGTAGAGGCCGG+CGG - tig0020315:141569-141588 None:intergenic 60.0%
CGTCAAAACGGGGAAGGGCA+AGG - tig0020315:141586-141605 None:intergenic 60.0%
GCGGTGGTTCCGTCAAAACG+GGG - tig0020315:141596-141615 None:intergenic 60.0%
TGACGGAACCACCGCATAGG+AGG + tig0020315:141601-141620 Msa1380010:five_prime_UTR 60.0%
CTTTGCGAGTTCGGAGGGGA+AGG + tig0020315:143603-143622 Msa1380010:intron 60.0%
GAGTTCGGAGGGGAAGGGAA+TGG + tig0020315:143609-143628 Msa1380010:intron 60.0%
TTCGGAGGGGAAGGGAATGG+AGG + tig0020315:143612-143631 Msa1380010:intron 60.0%
TCGGAGGGGAAGGGAATGGA+GGG + tig0020315:143613-143632 Msa1380010:intron 60.0%
GGGGAAGGGAATGGAGGGTT+TGG + tig0020315:143618-143637 Msa1380010:intron 60.0%
! TGGGAGGAGAGGGCTTTGAC+GGG + tig0020315:143679-143698 Msa1380010:intron 60.0%
!!! GAGGAGGGTTTTGGGGGGTT+TGG + tig0020315:143761-143780 Msa1380010:intron 60.0%
!!! AGGGTTTTGGGGGGTTTGGG+AGG + tig0020315:143765-143784 Msa1380010:intron 60.0%
!!! GGGTTTTGGGGGGTTTGGGA+GGG + tig0020315:143766-143785 Msa1380010:intron 60.0%
AGGGAAGGAAGGGCTCTGTG+GGG - tig0020315:143940-143959 None:intergenic 60.0%
GAAGGGAAGGAAGGGCTCTG+TGG - tig0020315:143942-143961 None:intergenic 60.0%
CGAGTTTGGAGGGGAAGGGA+AGG - tig0020315:143955-143974 None:intergenic 60.0%
!! CGCTAGTGAGGGGTATTCCG+GGG + tig0020315:144202-144221 Msa1380010:CDS 60.0%
CAAGGCCCGCAAAGAGAAGG+AGG + tig0020315:144455-144474 Msa1380010:CDS 60.0%
AGGGGAGAAGTGACCCGGAG+TGG + tig0020315:141479-141498 Msa1380010:five_prime_UTR 65.0%
GGCGCTAGGGTTGGGGTTGA+AGG - tig0020315:141548-141567 None:intergenic 65.0%
AACCCCAACCCTAGCGCCGC+CGG + tig0020315:141550-141569 Msa1380010:five_prime_UTR 70.0%
AGTAGAGGCCGGCGGCGCTA+GGG - tig0020315:141561-141580 None:intergenic 70.0%
AAGTAGAGGCCGGCGGCGCT+AGG - tig0020315:141562-141581 None:intergenic 70.0%
AGGCCGGCGGCGCTAGGGTT+GGG - tig0020315:141556-141575 None:intergenic 75.0%
! GGCCGGCGGCGCTAGGGTTG+GGG - tig0020315:141555-141574 None:intergenic 80.0%
! GAGGCCGGCGGCGCTAGGGT+TGG - tig0020315:141557-141576 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
tig0020315 gene 141371 144849 141371 ID=Msa1380010;Name=Msa1380010
tig0020315 mRNA 141371 144849 141371 ID=Msa1380010-mRNA-1;Parent=Msa1380010;Name=Msa1380010-mRNA-1;_AED=0.05;_eAED=0.05;_QI=254|1|1|1|0.5|0.66|3|310|106
tig0020315 exon 141371 141622 141371 ID=Msa1380010-mRNA-1:exon:6830;Parent=Msa1380010-mRNA-1
tig0020315 exon 144130 144329 144130 ID=Msa1380010-mRNA-1:exon:6831;Parent=Msa1380010-mRNA-1
tig0020315 exon 144417 144849 144417 ID=Msa1380010-mRNA-1:exon:6832;Parent=Msa1380010-mRNA-1
tig0020315 five_prime_UTR 141371 141622 141371 ID=Msa1380010-mRNA-1:five_prime_utr;Parent=Msa1380010-mRNA-1
tig0020315 five_prime_UTR 144130 144131 144130 ID=Msa1380010-mRNA-1:five_prime_utr;Parent=Msa1380010-mRNA-1
tig0020315 CDS 144132 144329 144132 ID=Msa1380010-mRNA-1:cds;Parent=Msa1380010-mRNA-1
tig0020315 CDS 144417 144539 144417 ID=Msa1380010-mRNA-1:cds;Parent=Msa1380010-mRNA-1
tig0020315 three_prime_UTR 144540 144849 144540 ID=Msa1380010-mRNA-1:three_prime_utr;Parent=Msa1380010-mRNA-1
Gene Sequence

>Msa1380010

ATGAGCATGAATGCGTTCAAGGCATATAAGTCCTGTGTCCCAATTGCTTATAGCCCCAATCTCTACATAACGCTAGTGAGGGGTATTCCGGGGACCAGAAAGCAACATAGGTGCACTTTGGAAGCACTACGCCTCGGCAAATGCAACCGAACTGTCATGCGATGGAACACACCTACTGTTAGGGGAATGATCCAGCAGGTCAAGAGGTTAGTTGTTGTTGAGACAGAGGAGATGTACAAGGCCCGCAAAGAGAAGGAGGAAACCCACAGAGCTCTTCGTCCTCCATTGGTCATAAACCATCAACCTGCTTCTTCTGCTTAG
Protein sequence

>Msa1380010

MSMNAFKAYKSCVPIAYSPNLYITLVRGIPGTRKQHRCTLEALRLGKCNRTVMRWNTPTVRGMIQQVKRLVVVETEEMYKARKEKEETHRALRPPLVINHQPASSA*