AlfalfaGEDB | Msa0439220

M. sativa cultivar zhongmu-4 / Msa0439220

KEGG: -
GO: -
PFAM: PF12697.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0439220	XP_013447467.1	93.860	342	18	2	1	342	1	339	0.0	645

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0439220	A0A072TXF9	93.860	342	18	2	1	342	1	339	0.0	645

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0053110	Msa0439220	0.808457	3.067987e-50	-8.615850e-47
Msa0439220	Msa0747780	0.802764	4.805818e-49	-8.615850e-47
Msa0439220	Msa1012340	0.933837	1.019996e-95	-8.615850e-47
Msa0439220	Msa1012440	0.914108	2.821332e-84	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0439220	MtrunA17_Chr7g0215511	93.860	342	18	2	1	342	1	339	0.0	645

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0439220	AT5G38360.1	68.025	319	95	4	27	342	17	331	2.18e-158	447
Msa0439220	AT5G38360.3	66.321	193	58	4	27	216	17	205	2.14e-89	267
Msa0439220	AT5G38360.2	64.571	175	55	4	27	198	17	187	3.29e-78	238

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Find 101 sgRNAs with CRISPR-Local

Find 393 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGAAAGAAGAAAGAA+AGG	0.303821	3_3:+4702841	None:intergenic
AAAGAAAAGAAAAGAAAAGT+TGG	0.408927	3_3:-4708891	None:intergenic
AAAGAAAAGTTGGATTGCAA+TGG	0.529625	3_3:-4708881	None:intergenic
AAAGAGAAAGAAGAAAGAAA+GGG	0.399357	3_3:+4702842	None:intergenic
AAAGTAGCAGAGGAAATGTT+TGG	0.436375	3_3:+4708480	Msa0439220:three_prime_UTR
AAATCCCAAAAGTAGTACTT+TGG	0.468505	3_3:-4708830	None:intergenic
AAATGAATGTCCGAGCAAGA+TGG	0.524223	3_3:-4705735	None:intergenic
AACAACATGATCCTCCATTG+TGG	0.583467	3_3:-4706939	None:intergenic
AACTAATGAAAGAAAGTTCA+CGG	0.475580	3_3:+4707633	Msa0439220:CDS
AACTTTCTTAAATCAAACAA+TGG	0.551344	3_3:-4707659	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAACCAGATTGAGAAAAGAA+AGG	-	chr3_3:4703212-4703231	None:intergenic	30.0%
TAAGAGTTTGAACACAATAC+TGG	-	chr3_3:4703400-4703419	None:intergenic	30.0%
AGTATTGTGTTCAAACTCTT+AGG	+	chr3_3:4703399-4703418	Msa0439220:intron	30.0%
GTATTGTGTTCAAACTCTTA+GGG	+	chr3_3:4703400-4703419	Msa0439220:intron	30.0%
GACACCATCATTATAATCTT+TGG	+	chr3_3:4703727-4703746	Msa0439220:intron	30.0%
ATGAGATGTTGAATGTTTGT+AGG	+	chr3_3:4703992-4704011	Msa0439220:intron	30.0%
TGAGATGTTGAATGTTTGTA+GGG	+	chr3_3:4703993-4704012	Msa0439220:intron	30.0%
AGTGGCATATGAAATTTATG+AGG	+	chr3_3:4704280-4704299	Msa0439220:intron	30.0%
GAATTGATAAAGGGCAATAA+AGG	+	chr3_3:4704549-4704568	Msa0439220:intron	30.0%
GGGCAATAAAGGAAAATAAT+AGG	+	chr3_3:4704560-4704579	Msa0439220:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	4702856	4708902	4702856	ID=Msa0439220;Name=Msa0439220
chr3_3	mRNA	4702856	4708902	4702856	ID=Msa0439220-mRNA-1;Parent=Msa0439220;Name=Msa0439220-mRNA-1;_AED=0.21;_eAED=0.21;_QI=50\|1\|1\|1\|0.87\|0.77\|9\|316\|342
chr3_3	exon	4702856	4703262	4702856	ID=Msa0439220-mRNA-1:exon:1046;Parent=Msa0439220-mRNA-1
chr3_3	exon	4705430	4705474	4705430	ID=Msa0439220-mRNA-1:exon:1047;Parent=Msa0439220-mRNA-1
chr3_3	exon	4705683	4705812	4705683	ID=Msa0439220-mRNA-1:exon:1048;Parent=Msa0439220-mRNA-1
chr3_3	exon	4706549	4706612	4706549	ID=Msa0439220-mRNA-1:exon:1049;Parent=Msa0439220-mRNA-1
chr3_3	exon	4706735	4706783	4706735	ID=Msa0439220-mRNA-1:exon:1050;Parent=Msa0439220-mRNA-1
chr3_3	exon	4706869	4706966	4706869	ID=Msa0439220-mRNA-1:exon:1051;Parent=Msa0439220-mRNA-1
chr3_3	exon	4707625	4707763	4707625	ID=Msa0439220-mRNA-1:exon:1052;Parent=Msa0439220-mRNA-1
chr3_3	exon	4708325	4708668	4708325	ID=Msa0439220-mRNA-1:exon:1053;Parent=Msa0439220-mRNA-1
chr3_3	exon	4708784	4708902	4708784	ID=Msa0439220-mRNA-1:exon:1054;Parent=Msa0439220-mRNA-1
chr3_3	five_prime_UTR	4702856	4702905	4702856	ID=Msa0439220-mRNA-1:five_prime_utr;Parent=Msa0439220-mRNA-1
chr3_3	CDS	4702906	4703262	4702906	ID=Msa0439220-mRNA-1:cds;Parent=Msa0439220-mRNA-1
chr3_3	CDS	4705430	4705474	4705430	ID=Msa0439220-mRNA-1:cds;Parent=Msa0439220-mRNA-1
chr3_3	CDS	4705683	4705812	4705683	ID=Msa0439220-mRNA-1:cds;Parent=Msa0439220-mRNA-1
chr3_3	CDS	4706549	4706612	4706549	ID=Msa0439220-mRNA-1:cds;Parent=Msa0439220-mRNA-1
chr3_3	CDS	4706735	4706783	4706735	ID=Msa0439220-mRNA-1:cds;Parent=Msa0439220-mRNA-1
chr3_3	CDS	4706869	4706966	4706869	ID=Msa0439220-mRNA-1:cds;Parent=Msa0439220-mRNA-1
chr3_3	CDS	4707625	4707763	4707625	ID=Msa0439220-mRNA-1:cds;Parent=Msa0439220-mRNA-1
chr3_3	CDS	4708325	4708471	4708325	ID=Msa0439220-mRNA-1:cds;Parent=Msa0439220-mRNA-1
chr3_3	three_prime_UTR	4708472	4708668	4708472	ID=Msa0439220-mRNA-1:three_prime_utr;Parent=Msa0439220-mRNA-1
chr3_3	three_prime_UTR	4708784	4708902	4708784	ID=Msa0439220-mRNA-1:three_prime_utr;Parent=Msa0439220-mRNA-1

Gene Sequence

>Msa0439220

ATGGCAATGACAGTGGGTCTTTCCGTGTGTTCAATTTTCAAAGTTTCTTATCATGATAATAATAATTACAACAATAAGAGAGTAACAGTAGCAGTATTGCAGAGAAAGGAGAAGATGAGTGTCCCTTACAATCTGAAGGAAGGGCAATCTCGTATTTTCCATGAGCTTCCTTCTGGTTTGAACATGGAAGTAATTGTCCAGAAGAAGAAGAAGAAGAATGAAAGTGAAAGTGAGTGTTCACCAGCTTTGGTATTTGTTCATGGAAGTTATCATGCTGCTTGGTGTTGGGCTGAACAGTGGTTGCCTTTCTTTTCTCAATCTGGTTATGATTGTTATGCTCTTTCTTTGCTTGGACAGGGTGAAAGTGATGAACCAGAAGATTCGGTTGCTGGTACACTTCAGACACACGCAACAGATGTTGCGGATTTTATTCATCGAAATATTCAATCGCCACCCATCTTGCTCGGACATTCATTTGGAGGACTAATTATTCAGTATTATATTTCCAATTTAGGAAATGACAAGCTCAAAGAAAATTTATACCCGGGGCTTAGAGGAGCTGTCCTTGTTTGTTCTGTACCTCCTTCTGGTAATAGTGGACTAGTTTGGCGATATCTAATTTCGAAACCAATTGCTGCCTTCAAGGTGACATACAGCTTGGCTGCAAAAGGTTTTCAAAGCTCTCTTTCTCTTTGCAAGGAGACATTTTTCTCACCCACAATGGAGGATCATGTTGTTAAAAGATATCAAGAACTAATGAAAGAAAGTTCACGGATGCCATTGTTTGATTTAAGAAAGTTGAATGCTTCGCTTCCAGTTCCTTTGGTGCCAAATCTCCCTGTTGAAGTTCTTGTTTTGGGCGCAAAGAATGACTTCATTGTGGATGCAGAAGGACTCAAAGAAACCGCACAGTTTTATGGTTTGTCACCAGTTTGTGTTGAAGGACTTGCTCATGACATGATGTTGGACCCTTCATGGGAGAAAGGTGCAGAAGTTATTCTTTCATGGCTAAAGGGTTTACACAAGTAG

Protein sequence

>Msa0439220

MAMTVGLSVCSIFKVSYHDNNNYNNKRVTVAVLQRKEKMSVPYNLKEGQSRIFHELPSGLNMEVIVQKKKKKNESESECSPALVFVHGSYHAAWCWAEQWLPFFSQSGYDCYALSLLGQGESDEPEDSVAGTLQTHATDVADFIHRNIQSPPILLGHSFGGLIIQYYISNLGNDKLKENLYPGLRGAVLVCSVPPSGNSGLVWRYLISKPIAAFKVTYSLAAKGFQSSLSLCKETFFSPTMEDHVVKRYQELMKESSRMPLFDLRKLNASLPVPLVPNLPVEVLVLGAKNDFIVDAEGLKETAQFYGLSPVCVEGLAHDMMLDPSWEKGAEVILSWLKGLHK*