AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0442660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0442660 MtrunA17_Chr3g0077331 93.261 460 20 4 53 508 65 517 0.0 818
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0442660 AT1G72040.1 61.896 517 168 9 4 508 15 514 0.0 610

Find 130 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAAGAAGAGAGAGATTT+TGG 0.153634 3_3:-12610834 None:intergenic
ATCCTTGCTGAAATCAATAT+TGG 0.197353 3_3:-12612919 None:intergenic
CTGTAACGTTGCGTCGCTTT+TGG 0.204642 3_3:-12610567 None:intergenic
CCTGATTTGTTGGCTATTCC+TGG 0.217222 3_3:+12611170 Msa0442660:CDS
GTTGTAGTACTCTGTTGTTC+AGG 0.275814 3_3:-12611023 None:intergenic
CTTCATCTTTCAGACTTAAT+AGG 0.282383 3_3:+12611105 Msa0442660:CDS
ATATAAAACCATCGGGAATA+AGG 0.284820 3_3:-12612525 None:intergenic
CAAAAGCGACGCAACGTTAC+AGG 0.285114 3_3:+12610568 Msa0442660:five_prime_UTR
TCCTTAACTTCATTCTTTGA+TGG 0.286569 3_3:-12612573 None:intergenic
TAGTGTGACGTGTTAGGGTT+AGG 0.289082 3_3:-12610908 None:intergenic
ATTCTTTGATGGCAAGTATC+AGG 0.291458 3_3:-12612562 None:intergenic
AATACACGGTCTCTTATATC+AGG 0.292967 3_3:-12612742 None:intergenic
TATTACTAGTGTGACGTGTT+AGG 0.311498 3_3:-12610914 None:intergenic
ATCTAGTCCTCCTGATTTGT+TGG 0.334574 3_3:+12611160 Msa0442660:CDS
TGCCACTTGCCAATTGGTTC+AGG 0.335895 3_3:-12612012 None:intergenic
CTCCATCTCATTCATCCAAT+TGG 0.351738 3_3:-12612452 None:intergenic
CTATTCCTGGTGTGGGTCCC+AGG 0.363835 3_3:+12611183 Msa0442660:CDS
ACGCAGTTCAATAGTTTCAT+TGG 0.370500 3_3:-12611971 None:intergenic
GAATGAAAGAAGAAGGGTTT+AGG 0.370611 3_3:-12610741 None:intergenic
CATCAAGCCTCTTCGATTGA+TGG 0.372708 3_3:+12612161 Msa0442660:CDS
TTCCTCTTCCTCTTCGTCTT+CGG 0.376189 3_3:+12611058 Msa0442660:CDS
TACTCCATGATTACCACTTT+GGG 0.385460 3_3:-12612674 None:intergenic
ATTACTAGTGTGACGTGTTA+GGG 0.396542 3_3:-12610913 None:intergenic
GCCATCAAAGAATGAAGTTA+AGG 0.403313 3_3:+12612572 Msa0442660:CDS
TTGTAGTACTCTGTTGTTCA+GGG 0.404090 3_3:-12611022 None:intergenic
TCCTTGCTGAAATCAATATT+GGG 0.404770 3_3:-12612918 None:intergenic
TGAAGTTGTGTTTGTAGAAG+AGG 0.408467 3_3:-12611087 None:intergenic
GTGAAGAGGTTGGGCGGTGA+AGG 0.412419 3_3:-12610671 None:intergenic
CCTGTTGTGTCTACCTTACC+TGG 0.415739 3_3:+12612502 Msa0442660:CDS
TAAAGAAGAATGAAAGAAGA+AGG 0.420561 3_3:-12610748 None:intergenic
TACTTTCAGCATGGTTCTTG+TGG 0.426754 3_3:-12611807 None:intergenic
TGGTAATCATGGAGTATTAT+CGG 0.432768 3_3:+12612681 Msa0442660:CDS
ATTTGATACCTTCTGAATGC+TGG 0.433730 3_3:-12612791 None:intergenic
ACATCCTGCCACTTGCCAAT+TGG 0.441088 3_3:-12612018 None:intergenic
AAAACATGTACGAACATAAG+AGG 0.448609 3_3:-12610877 None:intergenic
ATAGTTATCTTACTCCCTCT+TGG 0.449688 3_3:-12612949 None:intergenic
GAAGTGGATGATAATTCCTC+TGG 0.451397 3_3:+12611862 Msa0442660:CDS
CTCGTTCAGAAAGGTATTCA+AGG 0.453440 3_3:+12611218 Msa0442660:CDS
CCCTCCCAAAGTGGTAATCA+TGG 0.459472 3_3:+12612670 Msa0442660:CDS
CCAGGAATAGCCAACAAATC+AGG 0.460862 3_3:-12611170 None:intergenic
TTGTGTTGGGTGCAGTTCAT+TGG 0.461411 3_3:+12611733 Msa0442660:intron
ATACTCCATGATTACCACTT+TGG 0.465554 3_3:-12612675 None:intergenic
TGAAAGAAGAAGGGTTTAGG+AGG 0.471130 3_3:-12610738 None:intergenic
CGACTTACGTTCTGTGTTGA+GGG 0.471827 3_3:+12611907 Msa0442660:CDS
GAGCTGTTCATGAAGCCAAT+TGG 0.476172 3_3:+12612437 Msa0442660:CDS
CACAAGGAAGTGAAGAGGTT+GGG 0.480683 3_3:-12610680 None:intergenic
AACGGGAAAGCAAAGGAGAA+AGG 0.483822 3_3:-12610474 None:intergenic
CCAGGTAAGGTAGACACAAC+AGG 0.485266 3_3:-12612502 None:intergenic
AGATAAGCATCTATGACTCT+TGG 0.489241 3_3:+12612473 Msa0442660:CDS
AAGAAGAAGGGTTTAGGAGG+GGG 0.489403 3_3:-12610735 None:intergenic
ATTTGTTACCAGGGTGATGC+AGG 0.492135 3_3:+12612119 Msa0442660:CDS
TTGTTGGCTATTCCTGGTGT+GGG 0.492293 3_3:+12611176 Msa0442660:CDS
AAAGAAGAATGAAAGAAGAA+GGG 0.498651 3_3:-12610747 None:intergenic
AAAACTACGTATTTGTTACC+AGG 0.500178 3_3:+12612109 Msa0442660:CDS
CATGATTACCACTTTGGGAG+GGG 0.501556 3_3:-12612669 None:intergenic
AGTGTGACGTGTTAGGGTTA+GGG 0.501678 3_3:-12610907 None:intergenic
CTGAGATATATAAAACCATC+GGG 0.503584 3_3:-12612532 None:intergenic
GAGTATCTGCAGAGCTCTGT+TGG 0.508549 3_3:+12611778 Msa0442660:CDS
CAAACAACTCTACAAAGATA+AGG 0.510054 3_3:+12611253 Msa0442660:CDS
TCGAGGATATTGAAGTGGTC+AGG 0.510445 3_3:-12612045 None:intergenic
TTCAGAAAACTCGTTCAGAA+AGG 0.510465 3_3:+12611209 Msa0442660:CDS
GTAGTAGTAGTGGCGGGTGC+AGG 0.511213 3_3:-12610777 None:intergenic
TGAACCAATTGGCAAGTGGC+AGG 0.513776 3_3:+12612014 Msa0442660:CDS
TGCCGAAGACGAAGAGGAAG+AGG 0.515601 3_3:-12611060 None:intergenic
GCGACTTACGTTCTGTGTTG+AGG 0.517675 3_3:+12611906 Msa0442660:CDS
ACTTAATAGGAAGCAGCAAA+AGG 0.520353 3_3:+12611118 Msa0442660:CDS
TTTGTTGGCTATTCCTGGTG+TGG 0.521990 3_3:+12611175 Msa0442660:CDS
ATTGGCAAGTGGCAGGATGT+TGG 0.525269 3_3:+12612021 Msa0442660:CDS
TTTCATTGGCTATTCTCTGA+AGG 0.528274 3_3:-12611957 None:intergenic
ACGGGAAAGCAAAGGAGAAA+GGG 0.528583 3_3:-12610473 None:intergenic
AGGAAGAGAGAAGAAGAAGG+TGG 0.532736 3_3:+12612592 Msa0442660:CDS
CAGGAAAGAGAGTCTTCTGC+TGG 0.536413 3_3:+12612138 Msa0442660:CDS
AGAAGAAGAAGTAGTAGTAG+TGG 0.537877 3_3:-12610787 None:intergenic
AAACCATCGGGAATAAGGCC+AGG 0.544663 3_3:-12612520 None:intergenic
ATCGGGAATAAGGCCAGGTA+AGG 0.544995 3_3:-12612515 None:intergenic
AGAAGAAGTAGTAGTAGTGG+CGG 0.545454 3_3:-12610784 None:intergenic
AAATTCCTGGGACCCACACC+AGG 0.550512 3_3:-12611188 None:intergenic
CAAGGATATTGAAGCCAAGA+GGG 0.554039 3_3:+12612935 Msa0442660:CDS
TTACCTGGCCTTATTCCCGA+TGG 0.554673 3_3:+12612517 Msa0442660:CDS
TCCATGATTACCACTTTGGG+AGG 0.557575 3_3:-12612671 None:intergenic
TTAAGGAAGAGAGAAGAAGA+AGG 0.564660 3_3:+12612589 Msa0442660:CDS
AAAGAGTGTTGATGAAGAAG+TGG 0.565992 3_3:+12611846 Msa0442660:CDS
TGGCAAATCTAGTGATCAGA+TGG 0.567752 3_3:+12611753 Msa0442660:CDS
GCACAAGGAAGTGAAGAGGT+TGG 0.571132 3_3:-12610681 None:intergenic
TGTACGAACATAAGAGGCGT+TGG 0.572408 3_3:-12610871 None:intergenic
GGAGGTCGCGGAGATACTCT+AGG 0.573426 3_3:-12612621 None:intergenic
CCATGATTACCACTTTGGGA+GGG 0.576142 3_3:-12612670 None:intergenic
GAACGTTTAATAAGTAGCCA+TGG 0.576716 3_3:-12610957 None:intergenic
TAAAGAGTTGTCGACATGGA+GGG 0.578148 3_3:-12612716 None:intergenic
TCTGAGATATATAAAACCAT+CGG 0.581306 3_3:-12612533 None:intergenic
AAAGAAGAAGGGTTTAGGAG+GGG 0.582496 3_3:-12610736 None:intergenic
TGACGTGTTAGGGTTAGGGT+TGG 0.583359 3_3:-12610903 None:intergenic
GAGGTGGTTCCTGAACCAAT+TGG 0.589076 3_3:+12612003 Msa0442660:CDS
AAAGAGTTGTCGACATGGAG+GGG 0.589208 3_3:-12612715 None:intergenic
GAAGAAGTAGTAGTAGTGGC+GGG 0.590829 3_3:-12610783 None:intergenic
GAAAGAAGAAGGGTTTAGGA+GGG 0.592426 3_3:-12610737 None:intergenic
AGCCTCTTCGATTGATGGAG+AGG 0.592683 3_3:+12612166 Msa0442660:CDS
TCCACGAAAAGCATGAGAGC+TGG 0.595770 3_3:+12612641 Msa0442660:CDS
CTCTCTTTCCTGCATCACCC+TGG 0.596005 3_3:-12612127 None:intergenic
AGCCAATTGGATGAATGAGA+TGG 0.598931 3_3:+12612450 Msa0442660:CDS
CTCCTCTCCATCAATCGAAG+AGG 0.603846 3_3:-12612168 None:intergenic
CATGCATTCCAGCATTCAGA+AGG 0.612913 3_3:+12612783 Msa0442660:CDS
TAATAAGTAGCCATGGAAGG+TGG 0.617266 3_3:-12610950 None:intergenic
GGAAGATGCCGAAGACGAAG+AGG 0.617763 3_3:-12611066 None:intergenic
AAGTGGATGATAATTCCTCT+GGG 0.617994 3_3:+12611863 Msa0442660:CDS
TCACAGTCTAGAATCAATGC+AGG 0.618620 3_3:-12612894 None:intergenic
GTTTAATAAGTAGCCATGGA+AGG 0.622931 3_3:-12610953 None:intergenic
AAACTACGTATTTGTTACCA+GGG 0.623005 3_3:+12612110 Msa0442660:CDS
GTAAGTCGCTTCTTCTGCAT+AGG 0.624407 3_3:-12611892 None:intergenic
AAGCGTCGAGGATATTGAAG+TGG 0.625091 3_3:-12612050 None:intergenic
TGGTTATTCCCCTCCCAAAG+TGG 0.626744 3_3:+12612661 Msa0442660:CDS
ATACACGGTCTCTTATATCA+GGG 0.627573 3_3:-12612741 None:intergenic
AACAGCACAAGGAAGTGAAG+AGG 0.634339 3_3:-12610685 None:intergenic
CACCCTCGAGATAAAATACA+CGG 0.637012 3_3:-12612756 None:intergenic
AAGTAGTTATACTTTCAGCA+TGG 0.639328 3_3:-12611816 None:intergenic
GCAAGGATATTGAAGCCAAG+AGG 0.646490 3_3:+12612934 Msa0442660:CDS
GGTGTAAAGAGTTGTCGACA+TGG 0.647270 3_3:-12612720 None:intergenic
ACCCAATATTGATTTCAGCA+AGG 0.647767 3_3:+12612917 Msa0442660:CDS
TTCCTGAACCAATTGGCAAG+TGG 0.660138 3_3:+12612010 Msa0442660:CDS
ACTGCGTGATCTCGTCGAGG+TGG 0.663093 3_3:+12611987 Msa0442660:CDS
GTTGAGGGTAATATCAGTGT+TGG 0.665129 3_3:+12611922 Msa0442660:CDS
AAGGAAGTGAAGAGGTTGGG+CGG 0.672128 3_3:-12610677 None:intergenic
GGGGTGGAAATAACAGCACA+AGG 0.681411 3_3:-12610696 None:intergenic
TCTGAATGCTGGAATGCATG+TGG 0.686716 3_3:-12612780 None:intergenic
GTAAAGAGTTGTCGACATGG+AGG 0.688565 3_3:-12612717 None:intergenic
GGAATAGCCAACAAATCAGG+AGG 0.689568 3_3:-12611167 None:intergenic
TGAACTGCGTGATCTCGTCG+AGG 0.698431 3_3:+12611984 Msa0442660:CDS
CTGCATAGGCTGCTTCCCAG+AGG 0.699038 3_3:-12611878 None:intergenic
AAGGTGTATGCATACCTCTG+TGG 0.702954 3_3:-12612084 None:intergenic
ACAGAGTACTACAACAACAG+AGG 0.705483 3_3:+12611031 Msa0442660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCTAATAAACCTCAAAAA+CGG - chr3_3:12610495-12610514 None:intergenic 20.0%
!! AAATGTACCAATATTACAAA+GGG - chr3_3:12611587-12611606 None:intergenic 20.0%
!! TAAATGTACCAATATTACAA+AGG - chr3_3:12611588-12611607 None:intergenic 20.0%
!! CTTTGTATTAGAAAATCAAA+AGG - chr3_3:12611682-12611701 None:intergenic 20.0%
!!! TTTGATTTTCTAATACAAAG+TGG + chr3_3:12611682-12611701 Msa0442660:intron 20.0%
!! TTGAAGAAAAAATGTTGTAT+TGG - chr3_3:12612274-12612293 None:intergenic 20.0%
!!! ATGTTTTGAGAGTTTAAAAA+AGG + chr3_3:12612368-12612387 Msa0442660:intron 20.0%
!!! AATTACTTTTGATTACAAAC+AGG + chr3_3:12612869-12612888 Msa0442660:intron 20.0%
! TTCTAATAAACCTCAAAAAC+GGG - chr3_3:12610494-12610513 None:intergenic 25.0%
! AAAGAAGAATGAAAGAAGAA+GGG - chr3_3:12610750-12610769 None:intergenic 25.0%
! TAAAGAAGAATGAAAGAAGA+AGG - chr3_3:12610751-12610770 None:intergenic 25.0%
!!! CTTTACTATCTTCTGTTTTT+GGG + chr3_3:12611333-12611352 Msa0442660:intron 25.0%
! TAACTAATAGAAGACACATT+TGG + chr3_3:12611402-12611421 Msa0442660:intron 25.0%
! AACTAATAGAAGACACATTT+GGG + chr3_3:12611403-12611422 Msa0442660:intron 25.0%
! GAAATTAAATGATGTCCTAA+GGG - chr3_3:12611450-12611469 None:intergenic 25.0%
! TGAAATTAAATGATGTCCTA+AGG - chr3_3:12611451-12611470 None:intergenic 25.0%
! ATTATAGCAAACAGTCATTT+GGG - chr3_3:12611646-12611665 None:intergenic 25.0%
! TATTATAGCAAACAGTCATT+TGG - chr3_3:12611647-12611666 None:intergenic 25.0%
!!! AACAAATACGTAGTTTTGAA+AGG - chr3_3:12612106-12612125 None:intergenic 25.0%
! CACACAAGAATATGAAAAAA+TGG - chr3_3:12612228-12612247 None:intergenic 25.0%
!! TCTGAGATATATAAAACCAT+CGG - chr3_3:12612536-12612555 None:intergenic 25.0%
! AAAATCGTAAAATCAGACAT+GGG - chr3_3:12612825-12612844 None:intergenic 25.0%
! AAAAATCGTAAAATCAGACA+TGG - chr3_3:12612826-12612845 None:intergenic 25.0%
AAAACATGTACGAACATAAG+AGG - chr3_3:12610880-12610899 None:intergenic 30.0%
CTTCATCTTTCAGACTTAAT+AGG + chr3_3:12611105-12611124 Msa0442660:CDS 30.0%
CAAACAACTCTACAAAGATA+AGG + chr3_3:12611253-12611272 Msa0442660:CDS 30.0%
! GCTTTACTATCTTCTGTTTT+TGG + chr3_3:12611332-12611351 Msa0442660:intron 30.0%
CGTGTTAGTAATTTCATGAA+AGG + chr3_3:12611360-12611379 Msa0442660:intron 30.0%
ACTAATAGAAGACACATTTG+GGG + chr3_3:12611404-12611423 Msa0442660:intron 30.0%
TTCCTTAAACAATTCCCTTA+GGG + chr3_3:12611546-12611565 Msa0442660:intron 30.0%
!! AAAGTTTTGACCTTTGTGTT+GGG + chr3_3:12611720-12611739 Msa0442660:intron 30.0%
AAGTAGTTATACTTTCAGCA+TGG - chr3_3:12611819-12611838 None:intergenic 30.0%
! AAAACTACGTATTTGTTACC+AGG + chr3_3:12612109-12612128 Msa0442660:CDS 30.0%
! AAACTACGTATTTGTTACCA+GGG + chr3_3:12612110-12612129 Msa0442660:CDS 30.0%
! AATGTTGTATTGGCATGAAA+AGG - chr3_3:12612264-12612283 None:intergenic 30.0%
!!! CATTTTTTCTTCAAACGTGT+TGG + chr3_3:12612280-12612299 Msa0442660:intron 30.0%
ATTTATTTCCTTTCCCTGTT+TGG + chr3_3:12612316-12612335 Msa0442660:intron 30.0%
ATCTCAAATCAAACCAAACA+GGG - chr3_3:12612332-12612351 None:intergenic 30.0%
AATCTCAAATCAAACCAAAC+AGG - chr3_3:12612333-12612352 None:intergenic 30.0%
!! TGGTTTGATTTGAGATTAAC+AGG + chr3_3:12612336-12612355 Msa0442660:intron 30.0%
AATCTCACTGTGTTCAATAA+AGG + chr3_3:12612405-12612424 Msa0442660:intron 30.0%
ATATAAAACCATCGGGAATA+AGG - chr3_3:12612528-12612547 None:intergenic 30.0%
CTGAGATATATAAAACCATC+GGG - chr3_3:12612535-12612554 None:intergenic 30.0%
TCCTTAACTTCATTCTTTGA+TGG - chr3_3:12612576-12612595 None:intergenic 30.0%
! TGGTAATCATGGAGTATTAT+CGG + chr3_3:12612681-12612700 Msa0442660:CDS 30.0%
!! CATTCAGAAGGTATCAAATT+TGG + chr3_3:12612795-12612814 Msa0442660:intron 30.0%
TCCTTGCTGAAATCAATATT+GGG - chr3_3:12612921-12612940 None:intergenic 30.0%
ATCCTTGCTGAAATCAATAT+TGG - chr3_3:12612922-12612941 None:intergenic 30.0%
GAATGAAAGAAGAAGGGTTT+AGG - chr3_3:12610744-12610763 None:intergenic 35.0%
AGAAGAAGAAGTAGTAGTAG+TGG - chr3_3:12610790-12610809 None:intergenic 35.0%
ACTACTTCTTCTTCTTCTTC+TGG + chr3_3:12610795-12610814 Msa0442660:CDS 35.0%
!! ATTTTGGAAGAGGGAATGAA+AGG - chr3_3:12610821-12610840 None:intergenic 35.0%
!! AAGAGAGAGATTTTGGAAGA+GGG - chr3_3:12610830-12610849 None:intergenic 35.0%
! GAAGAAGAAGAGAGAGATTT+TGG - chr3_3:12610837-12610856 None:intergenic 35.0%
ATTACTAGTGTGACGTGTTA+GGG - chr3_3:12610916-12610935 None:intergenic 35.0%
TATTACTAGTGTGACGTGTT+AGG - chr3_3:12610917-12610936 None:intergenic 35.0%
GTTTAATAAGTAGCCATGGA+AGG - chr3_3:12610956-12610975 None:intergenic 35.0%
GAACGTTTAATAAGTAGCCA+TGG - chr3_3:12610960-12610979 None:intergenic 35.0%
TTGTAGTACTCTGTTGTTCA+GGG - chr3_3:12611025-12611044 None:intergenic 35.0%
TGAAGTTGTGTTTGTAGAAG+AGG - chr3_3:12611090-12611109 None:intergenic 35.0%
ACTTAATAGGAAGCAGCAAA+AGG + chr3_3:12611118-12611137 Msa0442660:CDS 35.0%
!!! ACGAGTTTTCTGAAATTCCT+GGG - chr3_3:12611203-12611222 None:intergenic 35.0%
!!! AACGAGTTTTCTGAAATTCC+TGG - chr3_3:12611204-12611223 None:intergenic 35.0%
TTCAGAAAACTCGTTCAGAA+AGG + chr3_3:12611209-12611228 Msa0442660:CDS 35.0%
TTCATGAAAGGACAAATTCG+AGG + chr3_3:12611372-12611391 Msa0442660:intron 35.0%
CTAATAGAAGACACATTTGG+GGG + chr3_3:12611405-12611424 Msa0442660:intron 35.0%
GTTCCTTAAACAATTCCCTT+AGG + chr3_3:12611545-12611564 Msa0442660:intron 35.0%
AGCACAACCCTTTGTAATAT+TGG + chr3_3:12611577-12611596 Msa0442660:intron 35.0%
GACACAACCTAACATATTCT+AGG + chr3_3:12611619-12611638 Msa0442660:intron 35.0%
TTTGGGTCCTAGAATATGTT+AGG - chr3_3:12611629-12611648 None:intergenic 35.0%
!! GAAAGTTTTGACCTTTGTGT+TGG + chr3_3:12611719-12611738 Msa0442660:intron 35.0%
!! AAAGAGTGTTGATGAAGAAG+TGG + chr3_3:12611846-12611865 Msa0442660:CDS 35.0%
AAGTGGATGATAATTCCTCT+GGG + chr3_3:12611863-12611882 Msa0442660:CDS 35.0%
! TTTCATTGGCTATTCTCTGA+AGG - chr3_3:12611960-12611979 None:intergenic 35.0%
ACGCAGTTCAATAGTTTCAT+TGG - chr3_3:12611974-12611993 None:intergenic 35.0%
TCATATTCTTGTGTGTAGTG+AGG + chr3_3:12612233-12612252 Msa0442660:intron 35.0%
CATATTCTTGTGTGTAGTGA+GGG + chr3_3:12612234-12612253 Msa0442660:intron 35.0%
AAATCAAACCAAACAGGGAA+AGG - chr3_3:12612327-12612346 None:intergenic 35.0%
AGATAAGCATCTATGACTCT+TGG + chr3_3:12612473-12612492 Msa0442660:CDS 35.0%
! ATTCTTTGATGGCAAGTATC+AGG - chr3_3:12612565-12612584 None:intergenic 35.0%
GCCATCAAAGAATGAAGTTA+AGG + chr3_3:12612572-12612591 Msa0442660:CDS 35.0%
TTAAGGAAGAGAGAAGAAGA+AGG + chr3_3:12612589-12612608 Msa0442660:CDS 35.0%
TACTCCATGATTACCACTTT+GGG - chr3_3:12612677-12612696 None:intergenic 35.0%
ATACTCCATGATTACCACTT+TGG - chr3_3:12612678-12612697 None:intergenic 35.0%
ATACACGGTCTCTTATATCA+GGG - chr3_3:12612744-12612763 None:intergenic 35.0%
AATACACGGTCTCTTATATC+AGG - chr3_3:12612745-12612764 None:intergenic 35.0%
ATTTGATACCTTCTGAATGC+TGG - chr3_3:12612794-12612813 None:intergenic 35.0%
!! ACCCAATATTGATTTCAGCA+AGG + chr3_3:12612917-12612936 Msa0442660:CDS 35.0%
TAAAGGAAGAAAAAGAGGGG+TGG - chr3_3:12610715-12610734 None:intergenic 40.0%
GGGTAAAGGAAGAAAAAGAG+GGG - chr3_3:12610718-12610737 None:intergenic 40.0%
GGGGTAAAGGAAGAAAAAGA+GGG - chr3_3:12610719-12610738 None:intergenic 40.0%
AAAGAAGAAGGGTTTAGGAG+GGG - chr3_3:12610739-12610758 None:intergenic 40.0%
GAAAGAAGAAGGGTTTAGGA+GGG - chr3_3:12610740-12610759 None:intergenic 40.0%
TGAAAGAAGAAGGGTTTAGG+AGG - chr3_3:12610741-12610760 None:intergenic 40.0%
AGAAGAAGTAGTAGTAGTGG+CGG - chr3_3:12610787-12610806 None:intergenic 40.0%
!! GAAGAGAGAGATTTTGGAAG+AGG - chr3_3:12610831-12610850 None:intergenic 40.0%
TAATAAGTAGCCATGGAAGG+TGG - chr3_3:12610953-12610972 None:intergenic 40.0%
GTTGTAGTACTCTGTTGTTC+AGG - chr3_3:12611026-12611045 None:intergenic 40.0%
! ACAGAGTACTACAACAACAG+AGG + chr3_3:12611031-12611050 Msa0442660:CDS 40.0%
! ATCTAGTCCTCCTGATTTGT+TGG + chr3_3:12611160-12611179 Msa0442660:CDS 40.0%
!! CTCGTTCAGAAAGGTATTCA+AGG + chr3_3:12611218-12611237 Msa0442660:CDS 40.0%
TGGCAAATCTAGTGATCAGA+TGG + chr3_3:12611753-12611772 Msa0442660:CDS 40.0%
TACTTTCAGCATGGTTCTTG+TGG - chr3_3:12611810-12611829 None:intergenic 40.0%
GAAGTGGATGATAATTCCTC+TGG + chr3_3:12611862-12611881 Msa0442660:CDS 40.0%
GTTGAGGGTAATATCAGTGT+TGG + chr3_3:12611922-12611941 Msa0442660:CDS 40.0%
AGCCAATTGGATGAATGAGA+TGG + chr3_3:12612450-12612469 Msa0442660:CDS 40.0%
CTCCATCTCATTCATCCAAT+TGG - chr3_3:12612455-12612474 None:intergenic 40.0%
TAAAGAGTTGTCGACATGGA+GGG - chr3_3:12612719-12612738 None:intergenic 40.0%
! AGACCGTGTATTTTATCTCG+AGG + chr3_3:12612753-12612772 Msa0442660:CDS 40.0%
! GACCGTGTATTTTATCTCGA+GGG + chr3_3:12612754-12612773 Msa0442660:CDS 40.0%
CACCCTCGAGATAAAATACA+CGG - chr3_3:12612759-12612778 None:intergenic 40.0%
! TCACAGTCTAGAATCAATGC+AGG - chr3_3:12612897-12612916 None:intergenic 40.0%
CAAGGATATTGAAGCCAAGA+GGG + chr3_3:12612935-12612954 Msa0442660:CDS 40.0%
ACGGGAAAGCAAAGGAGAAA+GGG - chr3_3:12610476-12610495 None:intergenic 45.0%
AACGGGAAAGCAAAGGAGAA+AGG - chr3_3:12610477-12610496 None:intergenic 45.0%
CCTCAAAAACGGGAAAGCAA+AGG - chr3_3:12610484-12610503 None:intergenic 45.0%
!! CCTTTGCTTTCCCGTTTTTG+AGG + chr3_3:12610481-12610500 Msa0442660:five_prime_UTR 45.0%
CACAAGGAAGTGAAGAGGTT+GGG - chr3_3:12610683-12610702 None:intergenic 45.0%
AACAGCACAAGGAAGTGAAG+AGG - chr3_3:12610688-12610707 None:intergenic 45.0%
GGGGGTAAAGGAAGAAAAAG+AGG - chr3_3:12610720-12610739 None:intergenic 45.0%
AAGAAGAAGGGTTTAGGAGG+GGG - chr3_3:12610738-12610757 None:intergenic 45.0%
GAAGAAGTAGTAGTAGTGGC+GGG - chr3_3:12610786-12610805 None:intergenic 45.0%
! TGTACGAACATAAGAGGCGT+TGG - chr3_3:12610874-12610893 None:intergenic 45.0%
AGTGTGACGTGTTAGGGTTA+GGG - chr3_3:12610910-12610929 None:intergenic 45.0%
TAGTGTGACGTGTTAGGGTT+AGG - chr3_3:12610911-12610930 None:intergenic 45.0%
ACACCAAAAACCACCTTCCA+TGG + chr3_3:12610940-12610959 Msa0442660:CDS 45.0%
! TAGCCATGGAAGGTGGTTTT+TGG - chr3_3:12610946-12610965 None:intergenic 45.0%
TTCCTCTTCCTCTTCGTCTT+CGG + chr3_3:12611058-12611077 Msa0442660:CDS 45.0%
GGAATAGCCAACAAATCAGG+AGG - chr3_3:12611170-12611189 None:intergenic 45.0%
CCAGGAATAGCCAACAAATC+AGG - chr3_3:12611173-12611192 None:intergenic 45.0%
!! CCTGATTTGTTGGCTATTCC+TGG + chr3_3:12611170-12611189 Msa0442660:CDS 45.0%
! TTTGTTGGCTATTCCTGGTG+TGG + chr3_3:12611175-12611194 Msa0442660:CDS 45.0%
! TTGTTGGCTATTCCTGGTGT+GGG + chr3_3:12611176-12611195 Msa0442660:CDS 45.0%
AGAGAGAGAGAGAAAGAGAG+AGG - chr3_3:12611292-12611311 None:intergenic 45.0%
ATAGAAGACACATTTGGGGG+TGG + chr3_3:12611408-12611427 Msa0442660:intron 45.0%
TAGAAGACACATTTGGGGGT+GGG + chr3_3:12611409-12611428 Msa0442660:intron 45.0%
GAATGCTAACAAGTGCCCTT+AGG + chr3_3:12611432-12611451 Msa0442660:intron 45.0%
CGCCCTAAGGGAATTGTTTA+AGG - chr3_3:12611551-12611570 None:intergenic 45.0%
ATGAACTGCACCCAACACAA+AGG - chr3_3:12611733-12611752 None:intergenic 45.0%
TTGTGTTGGGTGCAGTTCAT+TGG + chr3_3:12611733-12611752 Msa0442660:intron 45.0%
GTAAGTCGCTTCTTCTGCAT+AGG - chr3_3:12611895-12611914 None:intergenic 45.0%
CGACTTACGTTCTGTGTTGA+GGG + chr3_3:12611907-12611926 Msa0442660:CDS 45.0%
TTCCTGAACCAATTGGCAAG+TGG + chr3_3:12612010-12612029 Msa0442660:CDS 45.0%
TCGAGGATATTGAAGTGGTC+AGG - chr3_3:12612048-12612067 None:intergenic 45.0%
!! AAGCGTCGAGGATATTGAAG+TGG - chr3_3:12612053-12612072 None:intergenic 45.0%
! CTTTTTATGCTGAGCCACAG+AGG + chr3_3:12612070-12612089 Msa0442660:CDS 45.0%
AAGGTGTATGCATACCTCTG+TGG - chr3_3:12612087-12612106 None:intergenic 45.0%
! ATTTGTTACCAGGGTGATGC+AGG + chr3_3:12612119-12612138 Msa0442660:CDS 45.0%
CATCAAGCCTCTTCGATTGA+TGG + chr3_3:12612161-12612180 Msa0442660:CDS 45.0%
!!! AGAGGAGTGTTTTCAGTGAC+AGG + chr3_3:12612184-12612203 Msa0442660:CDS 45.0%
! GAGTGTTTTCAGTGACAGGA+TGG + chr3_3:12612188-12612207 Msa0442660:CDS 45.0%
GAGCTGTTCATGAAGCCAAT+TGG + chr3_3:12612437-12612456 Msa0442660:CDS 45.0%
AGGAAGAGAGAAGAAGAAGG+TGG + chr3_3:12612592-12612611 Msa0442660:CDS 45.0%
! CATGATTACCACTTTGGGAG+GGG - chr3_3:12612672-12612691 None:intergenic 45.0%
! CCATGATTACCACTTTGGGA+GGG - chr3_3:12612673-12612692 None:intergenic 45.0%
TCCATGATTACCACTTTGGG+AGG - chr3_3:12612674-12612693 None:intergenic 45.0%
AAAGAGTTGTCGACATGGAG+GGG - chr3_3:12612718-12612737 None:intergenic 45.0%
GTAAAGAGTTGTCGACATGG+AGG - chr3_3:12612720-12612739 None:intergenic 45.0%
GGTGTAAAGAGTTGTCGACA+TGG - chr3_3:12612723-12612742 None:intergenic 45.0%
TCTGAATGCTGGAATGCATG+TGG - chr3_3:12612783-12612802 None:intergenic 45.0%
CATGCATTCCAGCATTCAGA+AGG + chr3_3:12612783-12612802 Msa0442660:CDS 45.0%
GCAAGGATATTGAAGCCAAG+AGG + chr3_3:12612934-12612953 Msa0442660:CDS 45.0%
!!! TAACGTTGCGTCGCTTTTGG+AGG - chr3_3:12610567-12610586 None:intergenic 50.0%
!! CTGTAACGTTGCGTCGCTTT+TGG - chr3_3:12610570-12610589 None:intergenic 50.0%
CAAAAGCGACGCAACGTTAC+AGG + chr3_3:12610568-12610587 Msa0442660:five_prime_UTR 50.0%
AAGGAAGTGAAGAGGTTGGG+CGG - chr3_3:12610680-12610699 None:intergenic 50.0%
GCACAAGGAAGTGAAGAGGT+TGG - chr3_3:12610684-12610703 None:intergenic 50.0%
GGGGTGGAAATAACAGCACA+AGG - chr3_3:12610699-12610718 None:intergenic 50.0%
AAGGGTTTAGGAGGGGGTAA+AGG - chr3_3:12610732-12610751 None:intergenic 50.0%
TGACGTGTTAGGGTTAGGGT+TGG - chr3_3:12610906-12610925 None:intergenic 50.0%
AGAAGACACATTTGGGGGTG+GGG + chr3_3:12611410-12611429 Msa0442660:intron 50.0%
TGTGCTAACAAGCGCCCTAA+GGG - chr3_3:12611563-12611582 None:intergenic 50.0%
TTGTGCTAACAAGCGCCCTA+AGG - chr3_3:12611564-12611583 None:intergenic 50.0%
GAGTATCTGCAGAGCTCTGT+TGG + chr3_3:12611778-12611797 Msa0442660:CDS 50.0%
GCGACTTACGTTCTGTGTTG+AGG + chr3_3:12611906-12611925 Msa0442660:CDS 50.0%
GAGGTGGTTCCTGAACCAAT+TGG + chr3_3:12612003-12612022 Msa0442660:CDS 50.0%
! TGCCACTTGCCAATTGGTTC+AGG - chr3_3:12612015-12612034 None:intergenic 50.0%
TGAACCAATTGGCAAGTGGC+AGG + chr3_3:12612014-12612033 Msa0442660:CDS 50.0%
! ACATCCTGCCACTTGCCAAT+TGG - chr3_3:12612021-12612040 None:intergenic 50.0%
ATTGGCAAGTGGCAGGATGT+TGG + chr3_3:12612021-12612040 Msa0442660:CDS 50.0%
!! GCTCAGCATAAAAAGCGTCG+AGG - chr3_3:12612065-12612084 None:intergenic 50.0%
CAGGAAAGAGAGTCTTCTGC+TGG + chr3_3:12612138-12612157 Msa0442660:CDS 50.0%
AGCCTCTTCGATTGATGGAG+AGG + chr3_3:12612166-12612185 Msa0442660:CDS 50.0%
CTCCTCTCCATCAATCGAAG+AGG - chr3_3:12612171-12612190 None:intergenic 50.0%
CCAGGTAAGGTAGACACAAC+AGG - chr3_3:12612505-12612524 None:intergenic 50.0%
CCTGTTGTGTCTACCTTACC+TGG + chr3_3:12612502-12612521 Msa0442660:CDS 50.0%
ATCGGGAATAAGGCCAGGTA+AGG - chr3_3:12612518-12612537 None:intergenic 50.0%
!! TTACCTGGCCTTATTCCCGA+TGG + chr3_3:12612517-12612536 Msa0442660:CDS 50.0%
AAACCATCGGGAATAAGGCC+AGG - chr3_3:12612523-12612542 None:intergenic 50.0%
! AGCTCTCATGCTTTTCGTGG+AGG - chr3_3:12612642-12612661 None:intergenic 50.0%
! TCCACGAAAAGCATGAGAGC+TGG + chr3_3:12612641-12612660 Msa0442660:CDS 50.0%
! ACCAGCTCTCATGCTTTTCG+TGG - chr3_3:12612645-12612664 None:intergenic 50.0%
TGGTTATTCCCCTCCCAAAG+TGG + chr3_3:12612661-12612680 Msa0442660:CDS 50.0%
CCCTCCCAAAGTGGTAATCA+TGG + chr3_3:12612670-12612689 Msa0442660:CDS 50.0%
TGCCGAAGACGAAGAGGAAG+AGG - chr3_3:12611063-12611082 None:intergenic 55.0%
GGAAGATGCCGAAGACGAAG+AGG - chr3_3:12611069-12611088 None:intergenic 55.0%
AAATTCCTGGGACCCACACC+AGG - chr3_3:12611191-12611210 None:intergenic 55.0%
TGAACTGCGTGATCTCGTCG+AGG + chr3_3:12611984-12612003 Msa0442660:CDS 55.0%
CTCTCTTTCCTGCATCACCC+TGG - chr3_3:12612130-12612149 None:intergenic 55.0%
! CATGCTTTTCGTGGAGGTCG+CGG - chr3_3:12612636-12612655 None:intergenic 55.0%
!!! TGCGTCGCTTTTGGAGGTGG+CGG - chr3_3:12610561-12610580 None:intergenic 60.0%
!!! CGTTGCGTCGCTTTTGGAGG+TGG - chr3_3:12610564-12610583 None:intergenic 60.0%
GTGAAGAGGTTGGGCGGTGA+AGG - chr3_3:12610674-12610693 None:intergenic 60.0%
!! GTAGTAGTAGTGGCGGGTGC+AGG - chr3_3:12610780-12610799 None:intergenic 60.0%
CTATTCCTGGTGTGGGTCCC+AGG + chr3_3:12611183-12611202 Msa0442660:CDS 60.0%
CTGCATAGGCTGCTTCCCAG+AGG - chr3_3:12611881-12611900 None:intergenic 60.0%
ACTGCGTGATCTCGTCGAGG+TGG + chr3_3:12611987-12612006 Msa0442660:CDS 60.0%
GGAGGTCGCGGAGATACTCT+AGG - chr3_3:12612624-12612643 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 12610471 12612962 12610471 ID=Msa0442660;Name=Msa0442660
chr3_3 mRNA 12610471 12612962 12610471 ID=Msa0442660-mRNA-1;Parent=Msa0442660;Name=Msa0442660-mRNA-1;_AED=0.07;_eAED=0.07;_QI=189|1|0.75|1|1|1|4|0|508
chr3_3 exon 12610471 12611274 12610471 ID=Msa0442660-mRNA-1:exon:2716;Parent=Msa0442660-mRNA-1
chr3_3 exon 12611748 12612209 12611748 ID=Msa0442660-mRNA-1:exon:2717;Parent=Msa0442660-mRNA-1
chr3_3 exon 12612427 12612804 12612427 ID=Msa0442660-mRNA-1:exon:2718;Parent=Msa0442660-mRNA-1
chr3_3 exon 12612891 12612962 12612891 ID=Msa0442660-mRNA-1:exon:2719;Parent=Msa0442660-mRNA-1
chr3_3 five_prime_UTR 12610471 12610659 12610471 ID=Msa0442660-mRNA-1:five_prime_utr;Parent=Msa0442660-mRNA-1
chr3_3 CDS 12610660 12611274 12610660 ID=Msa0442660-mRNA-1:cds;Parent=Msa0442660-mRNA-1
chr3_3 CDS 12611748 12612209 12611748 ID=Msa0442660-mRNA-1:cds;Parent=Msa0442660-mRNA-1
chr3_3 CDS 12612427 12612804 12612427 ID=Msa0442660-mRNA-1:cds;Parent=Msa0442660-mRNA-1
chr3_3 CDS 12612891 12612962 12612891 ID=Msa0442660-mRNA-1:cds;Parent=Msa0442660-mRNA-1
Gene Sequence

>Msa0442660

ATGCATAAACTCCTTCACCGCCCAACCTCTTCACTTCCTTGTGCTGTTATTTCCACCCCTCTTTTTCTTCCTTTACCCCCTCCTAAACCCTTCTTCTTTCATTCTTCTTTAACAATGCCTGCACCCGCCACTACTACTACTTCTTCTTCTTCTTCTGGCCTTTCATTCCCTCTTCCAAAATCTCTCTCTTCTTCTTCTTCTTCTCGTTATTCCAACGCCTCTTATGTTCGTACATGTTTTTGTCCAACCCTAACCCTAACACGTCACACTAGTAATAATAACACCAAAAACCACCTTCCATGGCTACTTATTAAACGTTCTCAACATTCAAATGCTACTTCACAATCTTCTTTTCATAATAACCCTGAACAACAGAGTACTACAACAACAGAGGATGATTCCTCTTCCTCTTCGTCTTCGGCATCTTCCTCTTCTACAAACACAACTTCATCTTTCAGACTTAATAGGAAGCAGCAAAAGGCAGAATCATCATCATCATCATCTAGTCCTCCTGATTTGTTGGCTATTCCTGGTGTGGGTCCCAGGAATTTCAGAAAACTCGTTCAGAAAGGTATTCAAGGTGTTGCTCAACTCAAACAACTCTACAAAGATAAGTTCATTGGCAAATCTAGTGATCAGATGGTTGAGTATCTGCAGAGCTCTGTTGGAATTATCCACAAGAACCATGCTGAAAGTATAACTACTTTCATTAAAAAGAGTGTTGATGAAGAAGTGGATGATAATTCCTCTGGGAAGCAGCCTATGCAGAAGAAGCGACTTACGTTCTGTGTTGAGGGTAATATCAGTGTTGGCAAGACTACTTTCCTTCAGAGAATAGCCAATGAAACTATTGAACTGCGTGATCTCGTCGAGGTGGTTCCTGAACCAATTGGCAAGTGGCAGGATGTTGGACCTGACCACTTCAATATCCTCGACGCTTTTTATGCTGAGCCACAGAGGTATGCATACACCTTTCAAAACTACGTATTTGTTACCAGGGTGATGCAGGAAAGAGAGTCTTCTGCTGGCATCAAGCCTCTTCGATTGATGGAGAGGAGTGTTTTCAGTGACAGGATGGTTTTTGTACGAGCTGTTCATGAAGCCAATTGGATGAATGAGATGGAGATAAGCATCTATGACTCTTGGTTTGATCCTGTTGTGTCTACCTTACCTGGCCTTATTCCCGATGGTTTTATATATCTCAGAGCAAGTCCTGATACTTGCCATCAAAGAATGAAGTTAAGGAAGAGAGAAGAAGAAGGTGGAGTCAGCCTAGAGTATCTCCGCGACCTCCACGAAAAGCATGAGAGCTGGTTATTCCCCTCCCAAAGTGGTAATCATGGAGTATTATCGGTCAATAAGCTTCCCCTCCATGTCGACAACTCTTTACACCCTGATATAAGAGACCGTGTATTTTATCTCGAGGGTGACCACATGCATTCCAGCATTCAGAAGGTTCCTGCATTGATTCTAGACTGTGAACCCAATATTGATTTCAGCAAGGATATTGAAGCCAAGAGGGAGTAA
Protein sequence

>Msa0442660

MHKLLHRPTSSLPCAVISTPLFLPLPPPKPFFFHSSLTMPAPATTTTSSSSSGLSFPLPKSLSSSSSSRYSNASYVRTCFCPTLTLTRHTSNNNTKNHLPWLLIKRSQHSNATSQSSFHNNPEQQSTTTTEDDSSSSSSSASSSSTNTTSSFRLNRKQQKAESSSSSSSPPDLLAIPGVGPRNFRKLVQKGIQGVAQLKQLYKDKFIGKSSDQMVEYLQSSVGIIHKNHAESITTFIKKSVDEEVDDNSSGKQPMQKKRLTFCVEGNISVGKTTFLQRIANETIELRDLVEVVPEPIGKWQDVGPDHFNILDAFYAEPQRYAYTFQNYVFVTRVMQERESSAGIKPLRLMERSVFSDRMVFVRAVHEANWMNEMEISIYDSWFDPVVSTLPGLIPDGFIYLRASPDTCHQRMKLRKREEEGGVSLEYLRDLHEKHESWLFPSQSGNHGVLSVNKLPLHVDNSLHPDIRDRVFYLEGDHMHSSIQKVPALILDCEPNIDFSKDIEAKRE*