AlfalfaGEDB | Msa0454550

M. sativa cultivar zhongmu-4 / Msa0454550

KEGG: K13457
GO: GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363
PFAM: PF18052.4

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0454550	XP_003600246.2	91.102	944	76	5	1	938	1	942	0.0	1713

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0454550	sp\|Q39214\|RPM1_ARATH	29.958	948	609	19	21	947	13	926	5.66e-130	419

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0454550	G7J1I4	91.102	944	76	5	1	938	1	942	0.0	1713

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0213080	Msa0454550	0.827449	1.554848e-54	-8.615850e-47
Msa0296610	Msa0454550	0.800405	1.463765e-48	-8.615850e-47
Msa0361920	Msa0454550	0.832049	1.179788e-55	-8.615850e-47
Msa0437710	Msa0454550	0.805112	1.562070e-49	-8.615850e-47
Msa0454550	Msa0509970	0.814648	1.384395e-51	-8.615850e-47
Msa0454550	Msa0647000	0.815513	8.895665e-52	-8.615850e-47
Msa0454550	Msa1302150	0.815342	9.710395e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0454550	MtrunA17_Chr3g0101011	91.102	944	76	5	1	938	1	942	1713
Msa0454550	MtrunA17_Chr3g0101201	50.527	948	422	22	1	929	1	920	825
Msa0454550	MtrunA17_Chr3g0101271	51.425	912	403	19	28	930	7	887	810
Msa0454550	MtrunA17_Chr3g0101241	51.425	912	403	19	28	930	7	887	808
Msa0454550	MtrunA17_Chr3g0101221	49.948	955	429	23	1	932	1	929	805
Msa0454550	MtrunA17_Chr3g0101321	49.075	919	442	14	14	927	6	903	799
Msa0454550	MtrunA17_Chr3g0112511	50.271	923	424	19	14	929	6	900	798
Msa0454550	MtrunA17_Chr3g0101331	49.244	926	424	19	1	902	1	904	798
Msa0454550	MtrunA17_Chr3g0101231	48.574	947	450	17	1	929	1	928	796
Msa0454550	MtrunA17_Chr3g0100831	48.199	944	449	20	1	936	1	912	786

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0454550	AT3G07040.1	29.958	948	609	19	21	947	13	926	5.76e-131	419
Msa0454550	AT1G58400.3	26.586	914	599	28	1	888	1	868	1.52e-78	276
Msa0454550	AT1G58400.2	26.586	914	599	28	1	888	1	868	1.52e-78	276
Msa0454550	AT1G58390.1	26.584	963	598	33	12	928	4	903	1.47e-77	273
Msa0454550	AT1G58390.2	26.584	963	598	33	12	928	4	903	1.47e-77	273
Msa0454550	AT3G46710.1	30.045	882	520	33	25	877	17	830	1.36e-75	266
Msa0454550	AT5G43470.3	25.938	960	627	25	1	931	1	905	6.05e-75	265
Msa0454550	AT5G43470.2	25.938	960	627	25	1	931	1	905	6.05e-75	265
Msa0454550	AT5G43470.4	25.938	960	627	25	1	931	1	905	6.05e-75	265
Msa0454550	AT5G43470.1	25.938	960	627	25	1	931	1	905	6.05e-75	265

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Find 176 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATTTACAAACAAACA+GGG	0.647953	3_3:+45938793	Msa0454550:CDS
AAACAATGCTTCTTATACTT+TGG	0.280402	3_3:+45939356	Msa0454550:CDS
AAACATTTACAAACAAACAG+GGG	0.688861	3_3:+45938794	Msa0454550:CDS
AAAGATATTTACTTCATGAA+TGG	0.394415	3_3:+45939228	Msa0454550:CDS
AAATCCATCAAAATTGGTGA+AGG	0.564974	3_3:+45940589	Msa0454550:CDS
AAATTAACCAATTCATCCCT+TGG	0.426974	3_3:-45938591	None:intergenic
AAGATATTTACTTCATGAAT+GGG	0.419036	3_3:+45939229	Msa0454550:CDS
AAGCAACTGATCGCAAGCGA+AGG	0.617283	3_3:-45938037	None:intergenic
AAGCATTGTTTGAGATTGTA+AGG	0.360695	3_3:-45939344	None:intergenic
AAGGCATTCTTTGACTTGAA+TGG	0.371851	3_3:+45939101	Msa0454550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGGTTACTTCCTTTAATAG+TGG	-	chr3_3:45938087-45938106	None:intergenic	30.0%
GAAGTAACCAATATGATAAG+AGG	+	chr3_3:45938096-45938115	Msa0454550:CDS	30.0%
GTGTCAAAGATGATTTGATT+TGG	-	chr3_3:45938470-45938489	None:intergenic	30.0%
TGAGTTTCTCCAATGTAAAA+TGG	-	chr3_3:45938552-45938571	None:intergenic	30.0%
AATTAACCAATTCATCCCTT+GGG	-	chr3_3:45938593-45938612	None:intergenic	30.0%
AAATTAACCAATTCATCCCT+TGG	-	chr3_3:45938594-45938613	None:intergenic	30.0%
TCCATTTCAGAAATTTCACA+AGG	-	chr3_3:45938825-45938844	None:intergenic	30.0%
ATTTACTTCATGAATGGGAA+AGG	+	chr3_3:45939234-45939253	Msa0454550:CDS	30.0%
TGAAAATTTGAGTGTAGAGT+TGG	+	chr3_3:45939262-45939281	Msa0454550:CDS	30.0%
CCTGAGACTAATTAAACAAT+GGG	+	chr3_3:45939409-45939428	Msa0454550:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	45938018	45941315	45938018	ID=Msa0454550;Name=Msa0454550
chr3_3	mRNA	45938018	45941315	45938018	ID=Msa0454550-mRNA-1;Parent=Msa0454550;Name=Msa0454550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|0\|0.5\|1\|1\|1\|2\|119\|980
chr3_3	exon	45938018	45940814	45938018	ID=Msa0454550-mRNA-1:exon:8540;Parent=Msa0454550-mRNA-1
chr3_3	exon	45941051	45941315	45941051	ID=Msa0454550-mRNA-1:exon:8541;Parent=Msa0454550-mRNA-1
chr3_3	CDS	45938018	45940814	45938018	ID=Msa0454550-mRNA-1:cds;Parent=Msa0454550-mRNA-1
chr3_3	CDS	45941051	45941196	45941051	ID=Msa0454550-mRNA-1:cds;Parent=Msa0454550-mRNA-1
chr3_3	three_prime_UTR	45941197	45941315	45941197	ID=Msa0454550-mRNA-1:three_prime_utr;Parent=Msa0454550-mRNA-1

Gene Sequence

>Msa0454550

ATGTGTGACACTGTAATCTCCTTCGCTTGCGATCAGTTGCTTCCGTTAGCTCGGGACCATTTGCTTCCACTATTAAAGGAAGTAACCAATATGATAAGAGGTGTCCCAAAAGAAGTTGCAGACATGAAAAATGAACTAGAAAGCATTGAAGACTTCATCAACAATGCAGATAGAATGACCGAAGCTGAAGAAGACAATACGCGCGACGGAATCAAAGCAAAGATCAGACAGCTGAGAGAAGCATCTTTTCACATACAAGATGTCATTGATGAATATATGATATGTGAGGGACAGCAGCCTCATGATCCTGGATGTGCAGCTTTACTTCCTGAGATTAAAGACTTTTTCAAAACCAGGATTCTTCGTCTACAAATAGCTTACAAAATTCAGGACATTAAGTCACTAGTTAGTGCAATGGATGATACTGGTGGAAAAAACCATGGATTCCTCCAAATCAAATCATCTTTGACACGAGGATCAAGTAGTTCTGCAGCAAATGAAAACACCATATTCAACAACCTTAGAGAGGCTCCATTTTACATTGGAGAAACTCAAGTTGTGGGATTCAAAGCCCCAAGGGATGAATTGGTTAATTTGTTGATAAAGGGAAGAGAAGAGCTCACTGTCATCTCGGTGGTTGGGATGGGAGGGCAAGGTAAAACAACTCTTGCCAAGATAGTTTTTGACAGCAAAGATGTTATTGAATACTTTAATTTTCGTGTATGGATCACAGTGTCTCGACACACAGATGAAGAGTTGCTGAGGGACATGTTGCAAAACATTTACAAACAAACAGGGGAAGATCCTTGTGAAATTTCTGAAATGGATCGAAAGTCATTAATTGATAAAGTGAGAGACTTCTTGCAAAACAAAAGGTATATCATCTTGTTTGATGAAGTTTGGAATGAACAATTATGGAATGATATTGGATTTTCTTTGATTGATAGTAAAAATGGAAGTAGGGTATTAATCACAACTAGGAAGATTGATGTTGCCGTGTCTTGTAAGAGGTCTTCGTTTTTTCTTGAAGTGCATGAGTTGAAACCTTTGAATCATGAAAAATCTTTGGAGCTTTTTTATAAGAAGGCATTCTTTGACTTGAATGGACTATGTCCTGATAATCTTATGAATGTATCTTCTAAAATTGTTGAAAAATGTGAAGGTCTGCCACTTGCAATTGTGGCTATTGGTGGTCTTTTGTCTACCAAAGAAAGATATTTACTTCATGAATGGGAAAGGTTTAGTGAAAATTTGAGTGTAGAGTTGGACAACAATCCCAGTATACATGTTATAACAAAGATTTTAAGCTTTAGTTTTCATGATTTACCTTACAATCTCAAACAATGCTTCTTATACTTTGGAATATATCCTGGAAACTATGAAGTTAACGCCCTGAGACTAATTAAACAATGGGTAGCGGAAGGGTTTGTCAAAGAGGAAACAGGAAAAACGGTGGAAGAAGTTGCGGAACAATATTTAACAGAGTTGATCCATAGAAGGTTGGTTCTTGTATCTTCTTTTTCAAGTAATAGCAAAGCTAGAAGTTGTCATGTTCATGGCTTAATACGAGAAATGATCCTTGATAAAATTCAGGACCTAAGCTTTTGCAACTTTGCTCGCGATAATGAGGACCAGTCAGTCCTAAGCTTGATGACTCGTCGCCTAACAATATCAACCAGTTCCAACACTTTACTGTCAAGAAATGTTGAATGCTCAAACATTCGGTCACTGCATGTTTTCAAAAATGAAGAGCTACCAGATTGCTTTGTGGCAAGTATCCCTTCAAAGTTCAAATTACTAAAAGTATTTGATTTTGAAGATGTTTCACTGCATCATTATGTTCCAAAAAATCTAGGGGATTTATTCCATTTGAGATATTTAAGCTTTAGAAATACAAAAGTAAGGAATCTCCCAGGATCTATTGGTAAGCTTCATAACCTAGAGACCTTAGATTTAAGGCAAACTATGGTGCGCGAGTTACCAAAAGAGATTAACAAGCTTCAAAAGTTAAGGCACCTTCTAGCTTATGATACGTCTAAAGGTGTTGGATATGGAATTCAACTGAATGATGGCATTGGAGACATAGTGTCCCTACAAACGCTACGCGAAGTGGAAGCAGATCATGGCGGAGTTGAGCTGATTACCGATCTTGAGAGGCTCAAGAAGTTAAAAATGTTGGGGTTGACTAATGTTAAGAAAGAATACACAAAAGCTATATGTTCTTCCATATATGAGATGAAACACTTGGAGAAGCTATACATTGCTGCAATAAATAGACATGAAGTCATTGATTTTTTGAATTTCGATGTATCATTACATAAACTTCAGAAGCTTCGCCTTGTAGGGAAGTTGGGGAGGTTTCCATTTTGGATTAGAGAGCTACAAAGCCTTGTTAAATTGTCTTTGTCATATTCCATGTTAACTCATGATCCATTGATATCATTGAAAGATCTTCCGAATTTGTTATACCTTAGTATCCTCTTTTGTGCATATGAAGGTGAACATTTGCATTTTCAAGATGAAGGATTTAAGAGTCTCAAGCAACTGGTACTTAGGCGCTTGTATAATTTGAAATCCATCAAAATTGGTGAAGGAGCATTGTCCTCTCTAGAGAAGTTCAACTTAGTTAATATTCCACAACTGATGGAGGTGCCTTCTGGTGTCTACAACTTGCCAAGGCTTCAAGTATGTCATATCATAAACATGACTGATGAATTTGAGCAAAGCATTGATCGTGTTCGAGGACAACATCAATGGATCGTAGAGAAGGTTCCATTTGTAGGAATAGTTGACCGAAGAACTCATGGGGATAATTCTGATTCTCCTTGTGGCCTTTCAACAATGATGACATTCCATATTTCAGTGTCTGCTTTGATGCGTCAATTCTCAAATAAAGAGCAATATGAGACCTTTGAACAGATGGGTGATTCAAGACATTTTTGA

Protein sequence

>Msa0454550

MCDTVISFACDQLLPLARDHLLPLLKEVTNMIRGVPKEVADMKNELESIEDFINNADRMTEAEEDNTRDGIKAKIRQLREASFHIQDVIDEYMICEGQQPHDPGCAALLPEIKDFFKTRILRLQIAYKIQDIKSLVSAMDDTGGKNHGFLQIKSSLTRGSSSSAANENTIFNNLREAPFYIGETQVVGFKAPRDELVNLLIKGREELTVISVVGMGGQGKTTLAKIVFDSKDVIEYFNFRVWITVSRHTDEELLRDMLQNIYKQTGEDPCEISEMDRKSLIDKVRDFLQNKRYIILFDEVWNEQLWNDIGFSLIDSKNGSRVLITTRKIDVAVSCKRSSFFLEVHELKPLNHEKSLELFYKKAFFDLNGLCPDNLMNVSSKIVEKCEGLPLAIVAIGGLLSTKERYLLHEWERFSENLSVELDNNPSIHVITKILSFSFHDLPYNLKQCFLYFGIYPGNYEVNALRLIKQWVAEGFVKEETGKTVEEVAEQYLTELIHRRLVLVSSFSSNSKARSCHVHGLIREMILDKIQDLSFCNFARDNEDQSVLSLMTRRLTISTSSNTLLSRNVECSNIRSLHVFKNEELPDCFVASIPSKFKLLKVFDFEDVSLHHYVPKNLGDLFHLRYLSFRNTKVRNLPGSIGKLHNLETLDLRQTMVRELPKEINKLQKLRHLLAYDTSKGVGYGIQLNDGIGDIVSLQTLREVEADHGGVELITDLERLKKLKMLGLTNVKKEYTKAICSSIYEMKHLEKLYIAAINRHEVIDFLNFDVSLHKLQKLRLVGKLGRFPFWIRELQSLVKLSLSYSMLTHDPLISLKDLPNLLYLSILFCAYEGEHLHFQDEGFKSLKQLVLRRLYNLKSIKIGEGALSSLEKFNLVNIPQLMEVPSGVYNLPRLQVCHIINMTDEFEQSIDRVRGQHQWIVEKVPFVGIVDRRTHGDNSDSPCGLSTMMTFHISVSALMRQFSNKEQYETFEQMGDSRHF*