AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0459340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0459340 MtrunA17_Chr3g0107591 84.848 66 10 0 1 66 23 88 2.70e-34 119
Msa0459340 MtrunA17_Chr4g0008111 81.818 66 12 0 1 66 24 89 4.26e-33 115
Msa0459340 MtrunA17_Chr5g0404631 68.182 66 21 0 1 66 34 99 1.01e-28 104
Msa0459340 MtrunA17_Chr8g0372821 65.152 66 23 0 1 66 24 89 3.23e-25 95.1
Msa0459340 MtrunA17_Chr6g0475241 69.697 66 20 0 1 66 25 90 6.72e-25 94.4
Msa0459340 MtrunA17_Chr6g0475221 68.182 66 21 0 1 66 24 89 1.12e-23 90.9
Msa0459340 MtrunA17_Chr8g0372671 66.667 66 22 0 1 66 24 89 9.84e-23 88.6
Msa0459340 MtrunA17_Chr8g0343971 68.254 63 20 0 4 66 27 89 1.49e-22 87.8
Msa0459340 MtrunA17_Chr8g0343981 61.538 65 25 0 2 66 25 89 2.62e-21 84.3
Msa0459340 MtrunA17_Chr3g0144871 43.750 64 36 0 2 65 25 88 1.36e-14 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGATTTGGGTTGTTCTTT+TGG 0.175707 3_3:-55833827 Msa0459340:CDS
TCTGCAATTGCCAAGCTTCT+AGG 0.248424 3_3:+55833845 None:intergenic
AAGCTTGGCAATTGCAGATT+TGG 0.258208 3_3:-55833840 Msa0459340:CDS
TAGGAAGGTTGTTGCAATAT+AGG 0.308502 3_3:+55833864 None:intergenic
GTTGCAATATAGGTTGGTTA+TGG 0.326501 3_3:+55833874 None:intergenic
AGCTTGGCAATTGCAGATTT+GGG 0.370897 3_3:-55833839 Msa0459340:CDS
AGTTGTTGAAGTCATTCCTT+GGG 0.462705 3_3:+55833728 None:intergenic
AAGGTTGTTGCAATATAGGT+TGG 0.463361 3_3:+55833868 None:intergenic
CAACAACCTTCCTAGAAGCT+TGG 0.505968 3_3:-55833855 Msa0459340:CDS
AAGTTGTTGAAGTCATTCCT+TGG 0.509838 3_3:+55833727 None:intergenic
ATCAGAAATTATCAAAGTTG+TGG 0.510809 3_3:-55833783 Msa0459340:CDS
GATATCACCAAGAGAGTGCT+TGG 0.523447 3_3:+55833803 None:intergenic
GCATCATCATAAGTCTCCCA+AGG 0.622395 3_3:-55833744 Msa0459340:CDS
CAATTGCCAAGCTTCTAGGA+AGG 0.625878 3_3:+55833849 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAA+GGG + chr3_3:55834705-55834724 None:intergenic 0.0%
!! TATATATATATATATATATA+AGG + chr3_3:55834706-55834725 None:intergenic 0.0%
!!! AATTTTTAATAAAAAAAAAT+AGG + chr3_3:55835767-55835786 None:intergenic 0.0%
!!! AAATTTTTACATTTTTTGAA+AGG - chr3_3:55835113-55835132 Msa0459340:intron 10.0%
!!! AATTTTTACATTTTTTGAAA+GGG - chr3_3:55835114-55835133 Msa0459340:intron 10.0%
!!! TGTTTTAATTTTTATTGTTA+TGG - chr3_3:55835942-55835961 Msa0459340:intron 10.0%
!! AGCAATAAAATTATATGATT+AGG + chr3_3:55834982-55835001 None:intergenic 15.0%
!!! CATATAATTTTATTGCTTTT+TGG - chr3_3:55834985-55835004 Msa0459340:intron 15.0%
!! TATAAAATACTACTAATTTC+CGG + chr3_3:55835288-55835307 None:intergenic 15.0%
!! AAACTGATCTATATTATATA+TGG - chr3_3:55836014-55836033 Msa0459340:intron 15.0%
!! AACTCTTTCAAACAAAATAA+TGG - chr3_3:55833747-55833766 Msa0459340:CDS 20.0%
!!! AAGACATAGTTTCTTTTTTT+GGG - chr3_3:55833976-55833995 Msa0459340:intron 20.0%
!! TTAAATATAGTCATATGTGT+AGG + chr3_3:55834281-55834300 None:intergenic 20.0%
!! TATGACTATATTTAAATCTC+CGG - chr3_3:55834286-55834305 Msa0459340:intron 20.0%
!! AGATCACTTATTTCAATAAA+AGG - chr3_3:55834511-55834530 Msa0459340:intron 20.0%
!! GATCACTTATTTCAATAAAA+GGG - chr3_3:55834512-55834531 Msa0459340:intron 20.0%
!! TAAGTCTTTGATCTAAAAAA+AGG + chr3_3:55834859-55834878 None:intergenic 20.0%
!!! ATAGTTTTAAAGTTAACTCA+CGG - chr3_3:55834881-55834900 Msa0459340:intron 20.0%
!!! TCATATAATTTTATTGCTTC+TGG - chr3_3:55834944-55834963 Msa0459340:intron 20.0%
!!! AATAGTGAAAATTAGTCTTT+TGG + chr3_3:55835467-55835486 None:intergenic 20.0%
!!! TGTGTTTTCTTTTAAAACAA+AGG + chr3_3:55835530-55835549 None:intergenic 20.0%
!!! ACAACTCAATTTTTATCAAA+GGG + chr3_3:55835654-55835673 None:intergenic 20.0%
!!! AACAACTCAATTTTTATCAA+AGG + chr3_3:55835655-55835674 None:intergenic 20.0%
!! TTGTAGTAAACAAATATAAC+CGG - chr3_3:55836043-55836062 Msa0459340:intron 20.0%
! GATATAATATACCTGATGTA+AGG + chr3_3:55833832-55833851 None:intergenic 25.0%
! ATTGTTTAAGCATCTAAAAG+TGG - chr3_3:55833893-55833912 Msa0459340:CDS 25.0%
!! TTTTTGCATTGGAAATTGTA+CGG - chr3_3:55833919-55833938 Msa0459340:intron 25.0%
!!! CAAGACATAGTTTCTTTTTT+TGG - chr3_3:55833975-55833994 Msa0459340:intron 25.0%
!!! TTTTTTAGTCAAAAGCAAGT+GGG - chr3_3:55834059-55834078 Msa0459340:intron 25.0%
!! ACTTACTCTTTCATGTTTTT+AGG - chr3_3:55834095-55834114 Msa0459340:intron 25.0%
! CAAATTCGCTAAAAACAAAA+AGG - chr3_3:55834193-55834212 Msa0459340:intron 25.0%
! TCCATGTTAATAGCATAAAT+CGG - chr3_3:55834242-55834261 Msa0459340:intron 25.0%
! AAATAGAAAAACGAGTTAGA+TGG - chr3_3:55834535-55834554 Msa0459340:intron 25.0%
! AATAGAAAAACGAGTTAGAT+GGG - chr3_3:55834536-55834555 Msa0459340:intron 25.0%
! CTTTGATCTAAAAAAAGGAA+CGG + chr3_3:55834854-55834873 None:intergenic 25.0%
! ACACATATTTAAAAACACCA+AGG - chr3_3:55835020-55835039 Msa0459340:intron 25.0%
!!! CCAATACAATTTTTAGTTTC+CGG - chr3_3:55835266-55835285 Msa0459340:intron 25.0%
! TTGAAGTCACTTAAAATCAA+AGG - chr3_3:55835486-55835505 Msa0459340:intron 25.0%
!!! TCACTTGTTGTTAATTTTAC+AGG - chr3_3:55835674-55835693 Msa0459340:intron 25.0%
! TAACCGGTCTATATTATATA+TGG - chr3_3:55836059-55836078 Msa0459340:intron 25.0%
! AGACCATATATAATATAGAC+CGG + chr3_3:55836065-55836084 None:intergenic 25.0%
! AATTGTAAATATACAGCGAA+AGG - chr3_3:55836099-55836118 Msa0459340:intron 25.0%
! ATCAGAAATTATCAAAGTTG+TGG - chr3_3:55836246-55836265 Msa0459340:intron 25.0%
! AAAAGTGGCAATTTTTGCAT+TGG - chr3_3:55833908-55833927 Msa0459340:CDS 30.0%
TTCCTTAAACAATGTCCTTA+GGG - chr3_3:55833999-55834018 Msa0459340:intron 30.0%
ACCAATCACCACTAAAAAAA+GGG + chr3_3:55834040-55834059 None:intergenic 30.0%
AACCAATCACCACTAAAAAA+AGG + chr3_3:55834041-55834060 None:intergenic 30.0%
!!! GTTTTTTAGTCAAAAGCAAG+TGG - chr3_3:55834058-55834077 Msa0459340:intron 30.0%
!!! TACTCTTTCATGTTTTTAGG+TGG - chr3_3:55834098-55834117 Msa0459340:intron 30.0%
!!! ACTCTTTCATGTTTTTAGGT+GGG - chr3_3:55834099-55834118 Msa0459340:intron 30.0%
!! TTGTTTTTCTTGTTTCTCTG+AGG + chr3_3:55834412-55834431 None:intergenic 30.0%
TGTATGTATGTATATGAGTC+AGG - chr3_3:55834806-55834825 Msa0459340:intron 30.0%
!! GCTTTGTTTGAAAGTTTGAA+GGG - chr3_3:55835042-55835061 Msa0459340:intron 30.0%
!! TATTGGATGAGAGTTTTTAG+GGG + chr3_3:55835252-55835271 None:intergenic 30.0%
! GTATTGGATGAGAGTTTTTA+GGG + chr3_3:55835253-55835272 None:intergenic 30.0%
! TGTATTGGATGAGAGTTTTT+AGG + chr3_3:55835254-55835273 None:intergenic 30.0%
CCGGAAACTAAAAATTGTAT+TGG + chr3_3:55835269-55835288 None:intergenic 30.0%
GTGAAAGAAATAGAAGACAT+TGG + chr3_3:55835348-55835367 None:intergenic 30.0%
CCTTAGTGCACTTATTATTT+CGG - chr3_3:55835553-55835572 Msa0459340:intron 30.0%
ATCAAAGGGAAAAAGAACAA+GGG + chr3_3:55835640-55835659 None:intergenic 30.0%
TATCAAAGGGAAAAAGAACA+AGG + chr3_3:55835641-55835660 None:intergenic 30.0%
TGTACTTATATAACCGCAAA+TGG + chr3_3:55835980-55835999 None:intergenic 30.0%
ACTCAAGTACTACACATGAA+TGG - chr3_3:55833774-55833793 Msa0459340:CDS 35.0%
ATGCTTAAACAATGCCCTTA+GGG + chr3_3:55833886-55833905 None:intergenic 35.0%
GTTCCTTAAACAATGTCCTT+AGG - chr3_3:55833998-55834017 Msa0459340:intron 35.0%
!!! TAGCAAGACCCTTTTTTTAG+TGG - chr3_3:55834029-55834048 Msa0459340:intron 35.0%
!!! ACCCTTTTTTTAGTGGTGAT+TGG - chr3_3:55834036-55834055 Msa0459340:intron 35.0%
AAAAGGATGAATCTGTGAAC+AGG - chr3_3:55834210-55834229 Msa0459340:intron 35.0%
! GCCGATTTATGCTATTAACA+TGG + chr3_3:55834246-55834265 None:intergenic 35.0%
! AGAAACTAGGGTTTTTGTGA+CGG - chr3_3:55834614-55834633 Msa0459340:intron 35.0%
AAAAAGGAACGGTTGAGATT+TGG + chr3_3:55834843-55834862 None:intergenic 35.0%
!!! GTTTTAAAGTTAACTCACGG+TGG - chr3_3:55834884-55834903 Msa0459340:intron 35.0%
CAAACTTTCAAACAAAGCCT+TGG + chr3_3:55835040-55835059 None:intergenic 35.0%
!! GGCTTTGTTTGAAAGTTTGA+AGG - chr3_3:55835041-55835060 Msa0459340:intron 35.0%
TTGTTTGAAAGTTTGAAGGG+AGG - chr3_3:55835045-55835064 Msa0459340:intron 35.0%
TGTTTGAAAGTTTGAAGGGA+GGG - chr3_3:55835046-55835065 Msa0459340:intron 35.0%
!! GTTTTCGGGGAGAAAAATAT+AGG - chr3_3:55835078-55835097 Msa0459340:intron 35.0%
TAGAAGACATTGGAGAGATA+GGG + chr3_3:55835338-55835357 None:intergenic 35.0%
ATAGAAGACATTGGAGAGAT+AGG + chr3_3:55835339-55835358 None:intergenic 35.0%
GAGAGGAGGTTTGAAAAAAA+AGG + chr3_3:55835376-55835395 None:intergenic 35.0%
! CTTTGTTTGTGAGTTTGAAG+GGG + chr3_3:55835403-55835422 None:intergenic 35.0%
!! GCTTTGTTTGTGAGTTTGAA+GGG + chr3_3:55835404-55835423 None:intergenic 35.0%
!! AGGTTGGTAACTTACTTCTA+AGG + chr3_3:55835426-55835445 None:intergenic 35.0%
! TCTTTTGGTCAAAACCAAGT+GGG + chr3_3:55835452-55835471 None:intergenic 35.0%
CCGAAATAATAAGTGCACTA+AGG + chr3_3:55835556-55835575 None:intergenic 35.0%
GATCAGGACAACTCAATATA+AGG + chr3_3:55835608-55835627 None:intergenic 35.0%
GATGTGTTGTATTCCATTTG+CGG - chr3_3:55835964-55835983 Msa0459340:intron 35.0%
!! GTTGCAATATAGGTTGGTTA+TGG + chr3_3:55836158-55836177 None:intergenic 35.0%
AAGGTTGTTGCAATATAGGT+TGG + chr3_3:55836164-55836183 None:intergenic 35.0%
TAGGAAGGTTGTTGCAATAT+AGG + chr3_3:55836168-55836187 None:intergenic 35.0%
TGCAAACAACTCCTTACATC+AGG - chr3_3:55833818-55833837 Msa0459340:CDS 40.0%
AAATGCTAACAAGTGCCCTA+AGG - chr3_3:55833868-55833887 Msa0459340:CDS 40.0%
AATGCTAACAAGTGCCCTAA+GGG - chr3_3:55833869-55833888 Msa0459340:CDS 40.0%
GATGCTTAAACAATGCCCTT+AGG + chr3_3:55833887-55833906 None:intergenic 40.0%
TGCCCTAAGGACATTGTTTA+AGG + chr3_3:55834004-55834023 None:intergenic 40.0%
ATCCGGAAACATGGTTATTC+CGG + chr3_3:55834308-55834327 None:intergenic 40.0%
ATGACGCCAAAGTCATTGAT+TGG - chr3_3:55834339-55834358 Msa0459340:intron 40.0%
GCAGATCCAATCAATGACTT+TGG + chr3_3:55834348-55834367 None:intergenic 40.0%
! ACGAGTTAGATGGGTGATTT+TGG - chr3_3:55834545-55834564 Msa0459340:intron 40.0%
! CGAGTTAGATGGGTGATTTT+GGG - chr3_3:55834546-55834565 Msa0459340:intron 40.0%
TGGATGAAGGAGAAGAAACT+AGG - chr3_3:55834601-55834620 Msa0459340:intron 40.0%
GGATGAAGGAGAAGAAACTA+GGG - chr3_3:55834602-55834621 Msa0459340:intron 40.0%
! AGAGTTTTCGTTGTGAGCTT+AGG - chr3_3:55834662-55834681 Msa0459340:intron 40.0%
! TTTTCGTTGTGAGCTTAGGT+GGG - chr3_3:55834666-55834685 Msa0459340:intron 40.0%
GTTTGAAAGTTTGAAGGGAG+GGG - chr3_3:55835047-55835066 Msa0459340:intron 40.0%
!! GGCTTTGTTTGTGAGTTTGA+AGG + chr3_3:55835405-55835424 None:intergenic 40.0%
! GTCTTTTGGTCAAAACCAAG+TGG + chr3_3:55835453-55835472 None:intergenic 40.0%
AAGCTTGGCAATTGCAGATT+TGG - chr3_3:55836189-55836208 Msa0459340:intron 40.0%
AGCTTGGCAATTGCAGATTT+GGG - chr3_3:55836190-55836209 Msa0459340:intron 40.0%
!!! GCAGATTTGGGTTGTTCTTT+TGG - chr3_3:55836202-55836221 Msa0459340:intron 40.0%
! CTACACATGAATGGAAGCGT+TGG - chr3_3:55833783-55833802 Msa0459340:CDS 45.0%
TCTTGCTAACAAGTGCCCTA+AGG + chr3_3:55834017-55834036 None:intergenic 45.0%
CTCCGGAATAACCATGTTTC+CGG - chr3_3:55834303-55834322 Msa0459340:intron 45.0%
! GTCATTGATTGGATCTGCAC+TGG - chr3_3:55834350-55834369 Msa0459340:intron 45.0%
CACCAAAGAGGGGTGATTTA+GGG + chr3_3:55834583-55834602 None:intergenic 45.0%
TCTCCTTCATCCACCAAAGA+GGG + chr3_3:55834594-55834613 None:intergenic 45.0%
TTCTCCTTCATCCACCAAAG+AGG + chr3_3:55834595-55834614 None:intergenic 45.0%
! GTTTTCGTTGTGAGCTTAGG+TGG - chr3_3:55834665-55834684 Msa0459340:intron 45.0%
!! CGGTTGAGATTTGGTGTCAA+CGG + chr3_3:55834834-55834853 None:intergenic 45.0%
! GGAGGGGAAGAAAGAGTTTT+CGG - chr3_3:55835063-55835082 Msa0459340:intron 45.0%
! GAGGGGAAGAAAGAGTTTTC+GGG - chr3_3:55835064-55835083 Msa0459340:intron 45.0%
! AGGGGAAGAAAGAGTTTTCG+GGG - chr3_3:55835065-55835084 Msa0459340:intron 45.0%
ATTGGAGAGATAGGGCTTTG+GGG + chr3_3:55835330-55835349 None:intergenic 45.0%
CATTGGAGAGATAGGGCTTT+GGG + chr3_3:55835331-55835350 None:intergenic 45.0%
ACATTGGAGAGATAGGGCTT+TGG + chr3_3:55835332-55835351 None:intergenic 45.0%
AAAACCAAGTGGGTGTAGGT+TGG + chr3_3:55835442-55835461 None:intergenic 45.0%
!! ACAAGGGTACTCGTTTGATC+AGG + chr3_3:55835624-55835643 None:intergenic 45.0%
CAACAACCTTCCTAGAAGCT+TGG - chr3_3:55836174-55836193 Msa0459340:intron 45.0%
! CAATTGCCAAGCTTCTAGGA+AGG + chr3_3:55836183-55836202 None:intergenic 45.0%
TCTGCAATTGCCAAGCTTCT+AGG + chr3_3:55836187-55836206 None:intergenic 45.0%
! TTTTGGACCAAGCACTCTCT+TGG - chr3_3:55836219-55836238 Msa0459340:intron 45.0%
!! GATATCACCAAGAGAGTGCT+TGG + chr3_3:55836229-55836248 None:intergenic 45.0%
GCATCATCATAAGTCTCCCA+AGG - chr3_3:55836285-55836304 Msa0459340:intron 45.0%
!!! TATTATATCTAATTTTTTTT+AGG - chr3_3:55833843-55833862 Msa0459340:CDS 5.0%
!!! ATTATATCTAATTTTTTTTA+GGG - chr3_3:55833844-55833863 Msa0459340:CDS 5.0%
ACGTTGCAGATCCGGAAACA+TGG + chr3_3:55834317-55834336 None:intergenic 50.0%
ATGTTTCCGGATCTGCAACG+TGG - chr3_3:55834316-55834335 Msa0459340:intron 50.0%
GACCCTAAATCACCCCTCTT+TGG - chr3_3:55834578-55834597 Msa0459340:intron 50.0%
CCACCAAAGAGGGGTGATTT+AGG + chr3_3:55834584-55834603 None:intergenic 50.0%
CCTAAATCACCCCTCTTTGG+TGG - chr3_3:55834581-55834600 Msa0459340:intron 50.0%
CTCCTTCATCCACCAAAGAG+GGG + chr3_3:55834593-55834612 None:intergenic 50.0%
TGACGGCTCACAAGAGAGAA+AGG - chr3_3:55834631-55834650 Msa0459340:intron 50.0%
! TTGGAGAGATAGGGCTTTGG+GGG + chr3_3:55835329-55835348 None:intergenic 50.0%
TTTGAAGGGGAAGAGAGAGG+AGG + chr3_3:55835390-55835409 None:intergenic 50.0%
GAGTTTGAAGGGGAAGAGAG+AGG + chr3_3:55835393-55835412 None:intergenic 50.0%
GTTACCAACCTACACCCACT+TGG - chr3_3:55835435-55835454 Msa0459340:intron 50.0%
GGTCAAAACCAAGTGGGTGT+AGG + chr3_3:55835446-55835465 None:intergenic 50.0%
! GGTTGCCATCTAGAGTGCAT+AGG - chr3_3:55835695-55835714 Msa0459340:intron 50.0%
AGCAGCCTATGCACTCTAGA+TGG + chr3_3:55835703-55835722 None:intergenic 50.0%
CGTCATCCACGTTGCAGATC+CGG + chr3_3:55834325-55834344 None:intergenic 55.0%
! CACCCCTCTTTGGTGGATGA+AGG - chr3_3:55834588-55834607 Msa0459340:intron 55.0%
TCGTCGTCTTCGTGCGACGT+TGG + chr3_3:55834462-55834481 None:intergenic 60.0%
ACTCATGCGCCTGCGCACGC+GGG + chr3_3:55835817-55835836 None:intergenic 70.0%
GACTCATGCGCCTGCGCACG+CGG + chr3_3:55835818-55835837 None:intergenic 70.0%
TTGTGTGCGCCCGCGTGCGC+AGG - chr3_3:55835805-55835824 Msa0459340:intron 75.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 55833739 55836312 55833739 ID=Msa0459340;Name=Msa0459340
chr3_3 mRNA 55833739 55836312 55833739 ID=Msa0459340-mRNA-1;Parent=Msa0459340;Name=Msa0459340-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|0.5|0|0|2|0|67
chr3_3 exon 55836307 55836312 55836307 ID=Msa0459340-mRNA-1:exon:11104;Parent=Msa0459340-mRNA-1
chr3_3 exon 55833739 55833936 55833739 ID=Msa0459340-mRNA-1:exon:11103;Parent=Msa0459340-mRNA-1
chr3_3 CDS 55836307 55836312 55836307 ID=Msa0459340-mRNA-1:cds;Parent=Msa0459340-mRNA-1
chr3_3 CDS 55833739 55833936 55833739 ID=Msa0459340-mRNA-1:cds;Parent=Msa0459340-mRNA-1
Gene Sequence

>Msa0459340

ATGGAACGAAAGGTTATTTCTTTAACAAAACATTTGAGAGATAAAGCCATAACCAACCTATATTGCAACAACCTTCCTAGAAGCTTGGCAATTGCAGATTTGGGTTGTTCTTTTGGACCAAGCACTCTCTTGGTGATATCAGAAATTATCAAAGTTGTGGAAAAGCTTTGTCAAGAGCATCATCATAAGTCTCCCAAGGAATGA

Protein sequence

>Msa0459340

MERKVISLTKHLRDKAITNLYCNNLPRSLAIADLGCSFGPSTLLVISEIIKVVEKLCQEHHHKSPKE*