AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0469220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469220 MtrunA17_Chr3g0123821 99.550 222 1 0 1 222 1 222 2.06e-167 459
Msa0469220 MtrunA17_Chr8g0381841 89.732 224 21 2 1 222 1 224 3.77e-151 419
Msa0469220 MtrunA17_Chr1g0174281 73.394 218 56 2 6 221 5 222 1.49e-116 331
Msa0469220 MtrunA17_Chr7g0231131 72.558 215 59 0 6 220 5 219 6.45e-115 327
Msa0469220 MtrunA17_Chr8g0346421 65.438 217 67 2 8 220 18 230 3.92e-97 282
Msa0469220 MtrunA17_Chr7g0275991 66.184 207 67 2 11 216 15 219 4.57e-96 280
Msa0469220 MtrunA17_Chr4g0032201 63.208 212 74 1 10 221 6 213 1.77e-95 277
Msa0469220 MtrunA17_Chr2g0276941 61.321 212 78 2 11 221 10 218 1.59e-92 270
Msa0469220 MtrunA17_Chr3g0112681 62.802 207 75 1 14 220 10 214 7.89e-92 268
Msa0469220 MtrunA17_Chr2g0315551 59.633 218 84 4 4 221 2 215 1.30e-90 265
Msa0469220 MtrunA17_Chr8g0351861 56.561 221 90 3 7 221 6 226 3.47e-90 265
Msa0469220 MtrunA17_Chr2g0319951 64.865 185 64 1 4 188 2 185 2.34e-88 259
Msa0469220 MtrunA17_Chr2g0283131 57.674 215 84 3 9 221 6 215 6.64e-87 256
Msa0469220 MtrunA17_Chr4g0046261 55.814 215 88 2 9 221 6 215 2.69e-86 254
Msa0469220 MtrunA17_Chr2g0325091 55.814 215 91 1 7 221 2 212 6.10e-86 253
Msa0469220 MtrunA17_Chr4g0040781 55.814 215 88 2 9 221 6 215 1.19e-85 253
Msa0469220 MtrunA17_Chr1g0189361 57.820 211 86 1 14 221 12 222 1.33e-85 253
Msa0469220 MtrunA17_Chr7g0214241 55.924 211 84 2 15 221 14 219 9.16e-83 245
Msa0469220 MtrunA17_Chr7g0234201 56.250 208 85 2 15 221 12 214 1.41e-79 237
Msa0469220 MtrunA17_Chr6g0482721 51.887 212 99 1 10 221 6 214 4.28e-75 226
Msa0469220 MtrunA17_Chr8g0392931 53.271 214 94 3 10 221 8 217 3.96e-73 221
Msa0469220 MtrunA17_Chr2g0290891 46.193 197 101 2 15 211 5 196 1.83e-60 188
Msa0469220 MtrunA17_Chr6g0458081 51.515 165 80 0 14 178 4 168 8.85e-60 187
Msa0469220 MtrunA17_Chr8g0335471 44.811 212 105 2 10 221 2 201 2.14e-59 186
Msa0469220 MtrunA17_Chr4g0055651 43.868 212 106 2 10 221 2 200 5.40e-59 184
Msa0469220 MtrunA17_Chr8g0349711 51.829 164 79 0 15 178 5 168 6.06e-59 184
Msa0469220 MtrunA17_Chr7g0250531 43.396 212 107 2 10 221 2 200 1.23e-57 181
Msa0469220 MtrunA17_Chr2g0276931 42.723 213 110 2 9 221 2 202 8.49e-57 179
Msa0469220 MtrunA17_Chr4g0030911 42.584 209 111 3 14 221 13 213 1.82e-56 178
Msa0469220 MtrunA17_Chr8g0349681 50.610 164 81 0 15 178 383 546 1.32e-54 183
Msa0469220 MtrunA17_Chr8g0349681 47.027 185 97 1 10 193 169 353 1.31e-51 175
Msa0469220 MtrunA17_Chr8g0349681 51.923 156 75 0 15 170 12 167 1.47e-50 172
Msa0469220 MtrunA17_Chr1g0212521 42.105 209 113 3 14 221 13 214 1.54e-54 173
Msa0469220 MtrunA17_Chr1g0182321 47.059 170 89 1 9 177 8 177 2.00e-54 173
Msa0469220 MtrunA17_Chr7g0263071 47.879 165 85 1 14 177 13 177 6.12e-54 172
Msa0469220 MtrunA17_Chr1g0182301 46.471 170 90 1 9 177 8 177 6.39e-54 172
Msa0469220 MtrunA17_Chr2g0327911 44.578 166 90 2 14 177 13 178 4.59e-49 159
Msa0469220 MtrunA17_Chr3g0109721 44.509 173 90 1 1 173 1 167 4.66e-49 159
Msa0469220 MtrunA17_Chr1g0199331 47.904 167 83 2 5 170 23 186 8.04e-49 158
Msa0469220 MtrunA17_Chr5g0433561 39.819 221 111 5 1 221 1 199 1.43e-47 155
Msa0469220 MtrunA17_Chr8g0384311 38.140 215 119 5 9 221 6 208 1.23e-46 153
Msa0469220 MtrunA17_Chr8g0390561 37.745 204 110 2 18 221 11 197 2.34e-46 152
Msa0469220 MtrunA17_Chr1g0159481 37.264 212 120 5 12 221 13 213 2.84e-44 147
Msa0469220 MtrunA17_Chr3g0130271 37.383 214 119 6 11 221 7 208 1.02e-42 143
Msa0469220 MtrunA17_Chr8g0382221 34.848 198 113 2 17 198 10 207 1.12e-42 143
Msa0469220 MtrunA17_Chr3g0123971 39.610 154 93 0 17 170 10 163 1.28e-42 143
Msa0469220 MtrunA17_Chr5g0400131 39.610 154 93 0 17 170 10 163 2.43e-42 142
Msa0469220 MtrunA17_Chr7g0265001 45.283 159 85 2 14 170 14 172 2.61e-42 142
Msa0469220 MtrunA17_Chr1g0197331 42.286 175 98 3 9 180 8 182 3.82e-41 139
Msa0469220 MtrunA17_Chr4g0055081 37.662 154 96 0 17 170 10 163 5.25e-41 139
Msa0469220 MtrunA17_Chr1g0180291 42.012 169 95 3 14 180 12 179 4.59e-40 136
Msa0469220 MtrunA17_Chr8g0383261 38.182 165 95 3 16 173 12 176 5.93e-35 123
Msa0469220 MtrunA17_Chr6g0466301 39.394 165 93 3 16 173 8 172 7.97e-35 122
Msa0469220 MtrunA17_Chr6g0465491 39.394 165 93 3 16 173 8 172 7.97e-35 122
Msa0469220 MtrunA17_Chr4g0034871 35.714 196 117 4 16 202 8 203 8.50e-35 122
Msa0469220 MtrunA17_Chr1g0201121 38.788 165 94 3 16 173 8 172 1.40e-34 122
Msa0469220 MtrunA17_Chr2g0331691 37.576 165 96 3 16 173 10 174 6.36e-33 118
Msa0469220 MtrunA17_Chr4g0004661 37.576 165 96 3 16 173 64 228 6.67e-32 117
Msa0469220 MtrunA17_Chr6g0465471 38.514 148 84 4 33 173 7 154 1.85e-26 101
Msa0469220 MtrunA17_Chr6g0466161 38.514 148 84 4 33 173 7 154 1.89e-26 101
Msa0469220 MtrunA17_Chr3g0138111 28.986 207 130 5 11 214 7 199 6.63e-24 95.1
Msa0469220 MtrunA17_Chr3g0138101 28.986 207 130 5 11 214 7 199 7.85e-24 94.7
Msa0469220 MtrunA17_Chr3g0138121 29.126 206 129 5 11 213 7 198 2.92e-23 93.2
Msa0469220 MtrunA17_Chr2g0325101 33.333 165 96 5 18 170 8 170 3.94e-21 87.0
Msa0469220 MtrunA17_Chr4g0046211 35.537 121 75 3 18 137 8 126 5.22e-20 82.8
Msa0469220 MtrunA17_Chr3g0117931 32.121 165 98 5 18 170 10 172 6.30e-20 84.3
Msa0469220 MtrunA17_Chr6g0486041 28.877 187 119 5 18 192 8 192 1.11e-19 83.6
Msa0469220 MtrunA17_Chr4g0037021 35.294 119 74 3 18 135 8 124 2.20e-18 79.7
Msa0469220 MtrunA17_Chr8g0371501 31.325 166 98 6 18 170 8 170 1.09e-17 77.8
Msa0469220 MtrunA17_Chr5g0405791 31.325 166 98 6 18 170 8 170 3.82e-17 76.6
Msa0469220 MtrunA17_Chr6g0466431 37.374 99 53 1 16 114 8 97 5.05e-17 73.6
Msa0469220 MtrunA17_Chr6g0465611 37.374 99 53 1 16 114 8 97 5.05e-17 73.6
Msa0469220 MtrunA17_Chr1g0178291 31.447 159 102 3 19 177 431 582 2.25e-15 74.3
Msa0469220 MtrunA17_Chr5g0443631 26.744 172 114 5 17 180 105 272 1.88e-13 67.8
Msa0469220 MtrunA17_Chr2g0331841 44.828 58 32 0 85 142 2 59 1.09e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469220 AT5G65270.1 85.135 222 31 2 2 221 3 224 1.21e-138 387
Msa0469220 AT4G39990.1 80.569 211 38 2 12 221 13 221 5.99e-126 355
Msa0469220 AT3G12160.1 75.926 216 51 1 6 221 5 219 8.27e-124 350
Msa0469220 AT5G47960.1 74.528 212 53 1 10 220 9 220 2.59e-115 328
Msa0469220 AT1G09630.1 60.190 211 82 1 10 220 6 214 1.33e-94 276
Msa0469220 AT1G07410.1 61.321 212 77 1 10 221 6 212 5.22e-94 274
Msa0469220 AT4G18800.1 61.009 218 78 3 4 221 2 212 2.74e-92 270
Msa0469220 AT1G16920.1 60.092 218 82 3 4 221 2 214 5.74e-91 266
Msa0469220 AT5G45750.1 60.550 218 81 3 4 221 2 214 4.34e-90 264
Msa0469220 AT3G46830.1 69.143 175 54 0 10 184 6 180 2.08e-89 263
Msa0469220 AT3G46830.2 69.143 175 54 0 10 184 6 180 2.08e-89 263
Msa0469220 AT4G18430.1 56.744 215 86 2 9 221 6 215 2.76e-89 262
Msa0469220 AT5G59150.1 69.540 174 53 0 11 184 7 180 1.62e-88 260
Msa0469220 AT2G33870.1 57.674 215 85 2 9 221 6 216 3.34e-88 259
Msa0469220 AT5G47520.1 57.991 219 90 2 4 221 2 219 4.45e-88 259
Msa0469220 AT1G28550.1 58.605 215 83 2 9 221 6 216 1.33e-87 258
Msa0469220 AT1G06400.1 57.991 219 85 3 4 221 2 214 8.31e-87 256
Msa0469220 AT1G01200.1 60.369 217 75 5 11 220 23 235 9.89e-87 256
Msa0469220 AT5G60860.1 57.674 215 84 3 9 221 6 215 1.29e-86 255
Msa0469220 AT3G15060.1 56.744 215 86 3 9 221 6 215 1.59e-85 253
Msa0469220 AT1G05810.1 63.636 176 64 0 14 189 10 185 2.49e-82 244
Msa0469220 AT1G05810.2 63.636 176 64 0 14 189 56 231 3.67e-82 246
Msa0469220 AT2G43130.1 54.808 208 89 1 14 221 10 212 5.78e-81 241
Msa0469220 AT2G31680.1 61.364 176 68 0 14 189 10 185 2.85e-80 239
Msa0469220 AT3G07410.1 63.068 176 65 0 14 189 10 185 6.55e-79 236
Msa0469220 AT1G73640.1 51.339 224 96 4 11 221 8 231 7.06e-75 226
Msa0469220 AT1G18200.1 50.935 214 98 3 1 214 1 207 2.30e-74 225
Msa0469220 AT5G59150.2 70.400 125 37 0 60 184 5 129 9.38e-62 191
Msa0469220 AT4G17170.1 44.076 211 109 3 15 221 5 210 9.56e-61 189
Msa0469220 AT1G02130.1 46.226 212 102 3 10 221 2 201 1.15e-60 189
Msa0469220 AT4G17160.1 44.340 212 101 3 15 221 5 204 7.47e-60 187
Msa0469220 AT4G35860.1 43.192 213 110 2 14 221 4 210 2.01e-59 186
Msa0469220 AT5G47200.1 44.811 212 104 2 10 221 2 200 1.29e-57 181
Msa0469220 AT4G17530.1 44.811 212 104 2 10 221 2 200 1.33e-57 181
Msa0469220 AT3G11730.1 45.455 209 102 5 14 221 6 203 1.86e-56 178
Msa0469220 AT5G59840.1 41.627 209 114 3 14 221 13 214 2.10e-54 174
Msa0469220 AT3G46060.2 41.627 209 114 3 14 221 13 214 2.37e-54 173
Msa0469220 AT3G46060.3 41.627 209 114 3 14 221 13 214 2.37e-54 173
Msa0469220 AT3G46060.1 41.627 209 114 3 14 221 13 214 2.37e-54 173
Msa0469220 AT5G03520.1 41.121 214 117 3 9 221 8 213 7.33e-53 169
Msa0469220 AT3G53610.2 46.667 165 87 1 14 177 13 177 2.10e-52 168
Msa0469220 AT3G53610.3 46.667 165 87 1 14 177 13 177 2.10e-52 168
Msa0469220 AT3G53610.1 46.667 165 87 1 14 177 13 177 2.10e-52 168
Msa0469220 AT3G54840.2 51.948 154 73 1 18 170 35 188 1.23e-51 166
Msa0469220 AT3G09900.1 41.364 220 110 4 9 221 8 215 1.38e-51 166
Msa0469220 AT3G54840.1 51.948 154 73 1 18 170 35 188 1.75e-51 166
Msa0469220 AT3G54840.3 51.634 153 73 1 19 170 2 154 5.60e-51 163
Msa0469220 AT4G19640.1 37.557 221 116 3 1 221 1 199 6.21e-48 156
Msa0469220 AT1G43890.3 41.232 211 109 6 5 212 2 200 1.18e-46 154
Msa0469220 AT1G43890.1 41.232 211 109 6 5 212 2 200 1.18e-46 154
Msa0469220 AT1G43890.2 41.232 211 109 6 5 212 2 200 1.18e-46 154
Msa0469220 AT5G45130.1 42.775 173 93 1 1 173 1 167 7.15e-46 151
Msa0469220 AT4G35860.2 52.066 121 58 0 58 178 2 122 1.88e-44 146
Msa0469220 AT5G45130.2 44.156 154 86 0 20 173 22 175 6.84e-44 146
Msa0469220 AT2G22290.1 40.260 154 92 0 17 170 10 163 4.70e-43 144
Msa0469220 AT5G10260.1 38.961 154 94 0 17 170 10 163 6.15e-42 141
Msa0469220 AT2G44610.1 38.312 154 95 0 17 170 10 163 2.87e-41 140
Msa0469220 AT4G39890.1 38.065 155 95 1 17 170 10 164 5.17e-41 139
Msa0469220 AT5G03520.2 39.535 172 95 3 51 221 40 203 7.17e-41 139
Msa0469220 AT5G03530.2 36.986 219 125 6 5 221 2 209 1.32e-39 135
Msa0469220 AT5G03530.1 36.986 219 125 6 5 221 2 209 1.32e-39 135
Msa0469220 AT5G64990.1 35.404 161 104 0 17 177 8 168 4.73e-37 129
Msa0469220 AT4G19640.3 37.500 176 103 2 1 176 1 169 5.55e-37 128
Msa0469220 AT4G19640.2 42.958 142 75 2 1 142 1 136 4.15e-36 124
Msa0469220 AT1G22740.1 39.394 165 93 3 16 173 8 172 3.90e-35 124
Msa0469220 AT2G21880.1 39.394 165 93 3 16 173 9 173 4.96e-35 124
Msa0469220 AT3G18820.1 39.394 165 93 3 16 173 8 172 1.73e-34 122
Msa0469220 AT4G09720.1 40.000 165 92 3 16 173 8 172 2.07e-34 122
Msa0469220 AT5G64990.2 33.929 168 104 1 17 177 8 175 2.36e-34 122
Msa0469220 AT1G52280.1 37.647 170 99 3 16 178 8 177 3.58e-34 121
Msa0469220 AT1G49300.2 38.323 167 96 3 16 175 8 174 1.43e-33 120
Msa0469220 AT1G49300.1 38.323 167 96 3 16 175 8 174 1.43e-33 120
Msa0469220 AT4G09720.4 38.824 170 92 3 16 173 8 177 1.48e-33 120
Msa0469220 AT5G46025.1 54.286 105 47 1 78 181 6 110 4.38e-33 115
Msa0469220 AT4G09720.3 37.500 176 92 3 16 173 8 183 1.65e-32 117
Msa0469220 AT3G16100.1 36.970 165 97 3 16 173 8 172 1.18e-31 115
Msa0469220 AT3G09910.3 37.079 178 109 3 5 180 2 178 1.47e-31 114
Msa0469220 AT3G09910.1 37.079 178 109 3 5 180 2 178 1.47e-31 114
Msa0469220 AT3G09910.2 37.079 178 109 3 5 180 2 178 1.47e-31 114
Msa0469220 AT3G09910.5 40.252 159 92 3 14 170 11 168 4.47e-31 113
Msa0469220 AT3G09910.4 40.252 159 92 3 14 170 11 168 4.47e-31 113
Msa0469220 AT5G39620.1 31.604 212 125 5 17 221 6 204 6.44e-30 110
Msa0469220 AT2G21880.4 38.514 148 84 3 33 173 1 148 1.57e-27 103
Msa0469220 AT2G21880.3 38.514 148 84 3 33 173 1 148 1.57e-27 103
Msa0469220 AT5G55080.1 31.548 168 111 3 11 177 7 171 1.16e-25 100
Msa0469220 AT4G09720.6 41.481 135 72 3 46 173 4 138 1.46e-25 98.6
Msa0469220 AT4G09720.5 41.481 135 72 3 46 173 4 138 1.46e-25 98.6
Msa0469220 AT4G09720.2 41.481 135 72 3 46 173 4 138 1.46e-25 98.6
Msa0469220 AT2G21880.2 34.545 165 93 4 16 173 9 165 3.46e-25 98.2
Msa0469220 AT2G44690.1 35.758 165 92 5 18 170 20 182 2.45e-24 96.3
Msa0469220 AT5G20020.1 29.126 206 129 5 11 213 7 198 1.97e-23 94.0
Msa0469220 AT5G55190.1 29.126 206 129 5 11 213 7 198 3.67e-23 93.6
Msa0469220 AT5G20010.1 30.435 184 111 5 11 191 7 176 6.20e-23 92.8
Msa0469220 AT3G48040.1 32.121 165 98 5 18 170 10 172 1.22e-19 84.0
Msa0469220 AT1G20090.1 32.727 165 97 6 18 170 7 169 3.48e-19 82.4
Msa0469220 AT5G45970.1 31.361 169 102 5 18 174 8 174 3.93e-19 82.4
Msa0469220 AT4G35020.1 32.168 143 87 4 18 159 8 141 4.51e-19 82.0
Msa0469220 AT4G35020.2 32.168 143 87 4 18 159 8 141 4.51e-19 82.0
Msa0469220 AT4G35020.3 32.168 143 87 4 18 159 8 141 4.51e-19 82.0
Msa0469220 AT1G75840.1 32.121 165 98 6 18 170 8 170 5.22e-19 82.0
Msa0469220 AT5G62880.1 30.233 172 106 5 18 177 10 179 6.25e-19 82.0
Msa0469220 AT3G48040.2 36.364 121 74 3 18 137 10 128 3.90e-18 79.0
Msa0469220 AT3G51300.1 30.909 165 100 6 18 170 8 170 5.38e-18 79.3
Msa0469220 AT4G28950.1 27.222 180 119 4 17 186 7 184 1.80e-17 78.2
Msa0469220 AT5G62880.2 34.711 121 76 3 18 137 10 128 1.88e-17 77.4
Msa0469220 AT2G17800.2 30.909 165 100 6 18 170 8 170 2.35e-17 77.4
Msa0469220 AT2G17800.1 30.909 165 100 6 18 170 8 170 2.35e-17 77.4
Msa0469220 AT4G35950.1 30.909 165 100 6 18 170 8 170 2.89e-17 77.4
Msa0469220 AT4G08190.1 59.375 64 23 1 107 170 67 127 3.41e-17 75.1
Msa0469220 AT4G35020.4 33.333 114 73 3 18 130 8 119 3.06e-16 73.2
Msa0469220 AT4G35020.5 33.333 114 73 3 18 130 8 119 3.06e-16 73.2
Msa0469220 AT3G22950.1 29.167 120 79 3 17 135 18 132 4.92e-11 60.1
Msa0469220 AT3G22950.2 29.167 120 79 3 17 135 18 132 4.92e-11 60.1

Find 75 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTCTATATTAGGCTATT+TGG 0.161280 3_3:+71013721 None:intergenic
TGACAACACATGCTCTTCTT+AGG 0.168776 3_3:+71013995 None:intergenic
TTAATTAGCGGGTTTGAATT+TGG 0.271621 3_3:+71017208 None:intergenic
AATATAGTCATCATTCTTAT+AGG 0.279237 3_3:-71014277 Msa0469220:CDS
CCTGGCTGCTGATGAGAATC+AGG 0.310405 3_3:-71014083 Msa0469220:CDS
GTAATCAACCTCTCTATATT+AGG 0.319532 3_3:+71013713 None:intergenic
ATAGGTAGCCTGATTGGAAT+TGG 0.345742 3_3:-71013775 Msa0469220:three_prime_UTR
ATATTAGGCTATTTAGATAA+GGG 0.350505 3_3:+71013669 None:intergenic
CATACTACAGGGGCGCTGTT+GGG 0.366375 3_3:-71014387 Msa0469220:CDS
AGAAAGAAGAGAACCGTTCT+TGG 0.377427 3_3:+71016914 None:intergenic
GTGATCGAAGCTCGGACGTT+TGG 0.386754 3_3:+71014342 None:intergenic
ATGTAAATATACACGGGAAA+TGG 0.392454 3_3:+71013889 None:intergenic
AATTTCATAGGTAGCCTGAT+TGG 0.411337 3_3:-71013781 Msa0469220:three_prime_UTR
GACAACACATGCTCTTCTTA+GGG 0.416162 3_3:+71013996 None:intergenic
TTTGAATTTGGTTTGAAAAG+TGG 0.418073 3_3:+71017220 None:intergenic
AGAGTGTTAAGGCTCAGATC+TGG 0.425788 3_3:-71016948 Msa0469220:CDS
TAGTAACCATGCTCTATATT+AGG 0.426112 3_3:+71013654 None:intergenic
GGGCGCTGTTGGGGCAATGT+TGG 0.427779 3_3:-71014377 Msa0469220:CDS
TATATTAGGCTATTTAGATA+AGG 0.432288 3_3:+71013668 None:intergenic
CCTGATTCTCATCAGCAGCC+AGG 0.437809 3_3:+71014083 None:intergenic
TTCTTAGGGATTTCTTGTGC+AGG 0.438762 3_3:+71014010 None:intergenic
TGAGTTTCAAACTCGTACTT+TGG 0.440485 3_3:-71016983 Msa0469220:CDS
AAAGAATTTGCTGAGAAAGA+AGG 0.441025 3_3:-71014205 Msa0469220:CDS
GAAGATAGACTACGTCTTCA+AGG 0.447575 3_3:-71017103 Msa0469220:CDS
GCATACTACAGGGGCGCTGT+TGG 0.449830 3_3:-71014388 Msa0469220:CDS
TGAAGACGTAGTCTATCTTC+TGG 0.451825 3_3:+71017106 None:intergenic
ACAAGCGCTACTAATTTCAT+AGG 0.455963 3_3:-71013793 Msa0469220:three_prime_UTR
CGCAGTTCTTCTAACCATCT+AGG 0.458553 3_3:+71014316 None:intergenic
GTTTAATTTACTAGATTCCA+TGG 0.478421 3_3:-71013947 Msa0469220:three_prime_UTR
TGGAGATTAGTTTAATTAGC+GGG 0.481023 3_3:+71017197 None:intergenic
ACAATGTTCCAATTCCAATC+AGG 0.483149 3_3:+71013767 None:intergenic
GCTGATGAGAATCAGGGAAA+TGG 0.488775 3_3:-71014076 Msa0469220:CDS
TATTGAACATAAGAGTGTTA+AGG 0.489565 3_3:-71016959 Msa0469220:CDS
CAGTTACAAGTGCATACTAC+AGG 0.490649 3_3:-71014399 Msa0469220:CDS
GCTTCGATCACATACCTAGA+TGG 0.498381 3_3:-71014330 Msa0469220:CDS
ATTACCTCCGACTCCATCTC+AGG 0.499272 3_3:+71013831 None:intergenic
GGCAACTCAGCATCTCTGTC+CGG 0.499309 3_3:-71014055 Msa0469220:CDS
GGGATTTCTTGTGCAGGACC+AGG 0.502312 3_3:+71014016 None:intergenic
TGTTGATTTAAGACCTGAGA+TGG 0.509875 3_3:-71013844 Msa0469220:three_prime_UTR
AGCATGTGTTGTCAGTAATA+CGG 0.516064 3_3:-71013986 Msa0469220:exon
TTAATCGGTGATTCCGCCGT+TGG 0.519747 3_3:-71017075 Msa0469220:CDS
ATACTACAGGGGCGCTGTTG+GGG 0.525539 3_3:-71014386 Msa0469220:CDS
AGAGAGGTTGATTACTGCTA+TGG 0.526309 3_3:-71013705 Msa0469220:three_prime_UTR
GCTCAGATCTGGGACACTGC+TGG 0.529753 3_3:-71016937 Msa0469220:CDS
GAGTGTTAAGGCTCAGATCT+GGG 0.538288 3_3:-71016947 Msa0469220:CDS
GCAAGAACAATGGCGAGTGC+AGG 0.539693 3_3:-71017144 Msa0469220:exon
TTCTGTCAAAACAGTCATGA+AGG 0.542515 3_3:+71014132 None:intergenic
ACAATGGCGAGTGCAGGTTA+CGG 0.545612 3_3:-71017138 Msa0469220:CDS
GTGGAGATTAGTTTAATTAG+CGG 0.554778 3_3:+71017196 None:intergenic
ATGGCCAAACCATCAAAACT+TGG 0.558491 3_3:+71013908 None:intergenic
GTGGACTCAAAAGCTACAAT+CGG 0.568023 3_3:-71017009 Msa0469220:CDS
CATTGTCAACAAGAAGAACC+TGG 0.569186 3_3:-71014101 Msa0469220:CDS
TATTAGGCTATTTAGATAAG+GGG 0.572036 3_3:+71013670 None:intergenic
GGGACACTGCTGGCCAAGAA+CGG 0.580774 3_3:-71016927 Msa0469220:intron
AGGAAACAAAAGTGATCTTG+AGG 0.580983 3_3:-71014257 Msa0469220:CDS
CTAGGTATGTGATCGAAGCT+CGG 0.584273 3_3:+71014334 None:intergenic
GAAAATCTCAGATACTAGCT+CGG 0.589018 3_3:-71017053 Msa0469220:CDS
TTGAATGATGTAAATATACA+CGG 0.596315 3_3:+71013882 None:intergenic
TGAATGATGTAAATATACAC+GGG 0.603917 3_3:+71013883 None:intergenic
AGTTACAAGTGCATACTACA+GGG 0.605565 3_3:-71014398 Msa0469220:CDS
AAATAGCCTAATATAGAGCA+TGG 0.613837 3_3:-71013660 Msa0469220:three_prime_UTR
TTTAAGACCTGAGATGGAGT+CGG 0.620598 3_3:-71013838 Msa0469220:three_prime_UTR
CTGGCTGCTGATGAGAATCA+GGG 0.625486 3_3:-71014082 Msa0469220:CDS
CCAAATAGCCTAATATAGAG+AGG 0.634926 3_3:-71013721 Msa0469220:three_prime_UTR
TGACTATATTCTTGTCAGCA+TGG 0.639191 3_3:+71014290 None:intergenic
GAAATCAAAAGCAAGAACAA+TGG 0.640264 3_3:-71017154 Msa0469220:exon
ATTAGTTGCTTGCAGCGCAG+AGG 0.640396 3_3:+71014165 None:intergenic
GCAAGCAACTAATGTTGAGA+CGG 0.646912 3_3:-71014155 Msa0469220:CDS
GGTTGATTACTGCTATGGTG+TGG 0.657425 3_3:-71013700 Msa0469220:three_prime_UTR
AAGACCTGAGATGGAGTCGG+AGG 0.667296 3_3:-71013835 Msa0469220:three_prime_UTR
GTCATACGAACATAGAACCA+TGG 0.670149 3_3:+71013930 None:intergenic
TGCAAGAAACGACTTCAGCG+TGG 0.696247 3_3:-71017028 Msa0469220:CDS
GTTACAAGTGCATACTACAG+GGG 0.696627 3_3:-71014397 Msa0469220:CDS
GCAGCGTGCAGTACCCACAG+AGG 0.703658 3_3:-71014233 Msa0469220:CDS
GATAGACTACGTCTTCAAGG+TGG 0.714276 3_3:-71017100 Msa0469220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTTTTTTTAAAAAACT+CGG + chr3_3:71014546-71014565 None:intergenic 10.0%
!!! AATTATATCAAATTTAAAAC+TGG + chr3_3:71014973-71014992 None:intergenic 10.0%
!!! GAAGAAAAACATAAATTTTA+TGG - chr3_3:71015221-71015240 Msa0469220:intron 15.0%
!!! ACTGTTGATAAATAAATATT+TGG + chr3_3:71015591-71015610 None:intergenic 15.0%
!! TGTACTTGTAATTTAAATAT+TGG + chr3_3:71016340-71016359 None:intergenic 15.0%
!!! GTTTATTTATCTGAAGTTAA+TGG - chr3_3:71014066-71014085 Msa0469220:CDS 20.0%
!!! ACACCAATTTTTTTTTATTG+AGG - chr3_3:71014175-71014194 Msa0469220:CDS 20.0%
!! AGTCCTCAATAAAAAAAAAT+TGG + chr3_3:71014181-71014200 None:intergenic 20.0%
!! AAAAAAATCTAGTATGTGAA+TGG - chr3_3:71014556-71014575 Msa0469220:intron 20.0%
!!! ATGATTTTTATTTCAAAGAG+TGG + chr3_3:71016079-71016098 None:intergenic 20.0%
!!! ATTTTCAAAATCAGCAATTT+TGG - chr3_3:71016151-71016170 Msa0469220:intron 20.0%
!!! GTTTTGTACTATTTTTTCTA+CGG - chr3_3:71016264-71016283 Msa0469220:intron 20.0%
!!! GTTTTGTCTGATTTTATAAA+TGG - chr3_3:71016376-71016395 Msa0469220:intron 20.0%
!! AATATAGTCATCATTCTTAT+AGG - chr3_3:71016543-71016562 Msa0469220:intron 20.0%
!! TTGAATGATGTAAATATACA+CGG + chr3_3:71016941-71016960 None:intergenic 20.0%
!! ATATTAGGCTATTTAGATAA+GGG + chr3_3:71017154-71017173 None:intergenic 20.0%
!! TATATTAGGCTATTTAGATA+AGG + chr3_3:71017155-71017174 None:intergenic 20.0%
!!! TTTGAATTTGGTTTGAAAAG+TGG + chr3_3:71013603-71013622 None:intergenic 25.0%
!!! TATTGAACATAAGAGTGTTA+AGG - chr3_3:71013861-71013880 Msa0469220:three_prime_UTR 25.0%
!!! TTGTTTATTGTTTTTGCCTA+TGG - chr3_3:71014374-71014393 Msa0469220:CDS 25.0%
! TATGTGAATTAACCCATTTA+GGG - chr3_3:71014495-71014514 Msa0469220:intron 25.0%
!! TGATTTTACCATAAGTTCAA+AGG + chr3_3:71014805-71014824 None:intergenic 25.0%
!!! AGCATATTTTGATTTGAAAC+CGG + chr3_3:71014829-71014848 None:intergenic 25.0%
!!! ATGTATTGACTGATTTCAAT+TGG - chr3_3:71014904-71014923 Msa0469220:intron 25.0%
!! TCAAATTTAAAACTGGAATG+TGG + chr3_3:71014966-71014985 None:intergenic 25.0%
! GAACCAAATCATTTATTCAT+GGG + chr3_3:71015474-71015493 None:intergenic 25.0%
!! ACCTTTTGTACATATTAACA+GGG + chr3_3:71016013-71016032 None:intergenic 25.0%
!! AACCTTTTGTACATATTAAC+AGG + chr3_3:71016014-71016033 None:intergenic 25.0%
!! TATCAATAACACGTTTTCAA+AGG + chr3_3:71016128-71016147 None:intergenic 25.0%
! TTGGTTGAGTAAAAAAACAT+GGG + chr3_3:71016226-71016245 None:intergenic 25.0%
! ATTGGTTGAGTAAAAAAACA+TGG + chr3_3:71016227-71016246 None:intergenic 25.0%
!! GTTTAATTTACTAGATTCCA+TGG - chr3_3:71016873-71016892 Msa0469220:intron 25.0%
! TGAATGATGTAAATATACAC+GGG + chr3_3:71016940-71016959 None:intergenic 25.0%
! TATTAGGCTATTTAGATAAG+GGG + chr3_3:71017153-71017172 None:intergenic 25.0%
!! TTAATTAGCGGGTTTGAATT+TGG + chr3_3:71013615-71013634 None:intergenic 30.0%
!! TGGAGATTAGTTTAATTAGC+GGG + chr3_3:71013626-71013645 None:intergenic 30.0%
!! GTGGAGATTAGTTTAATTAG+CGG + chr3_3:71013627-71013646 None:intergenic 30.0%
!! TTGGTTTTCGATTTTGAATG+TGG + chr3_3:71013646-71013665 None:intergenic 30.0%
!!! TGTTCTTGCTTTTGATTTCT+TGG + chr3_3:71013665-71013684 None:intergenic 30.0%
GAAATCAAAAGCAAGAACAA+TGG - chr3_3:71013666-71013685 Msa0469220:three_prime_UTR 30.0%
!! TAGTATCTGAGATTTTCCAA+CGG + chr3_3:71013764-71013783 None:intergenic 30.0%
ACATCAGTCAAACTAATTCA+AGG - chr3_3:71014226-71014245 Msa0469220:CDS 30.0%
GTGTCAAACAAATTCAAGAT+TGG + chr3_3:71014278-71014297 None:intergenic 30.0%
! TTTTTGCCTATGGTTTCTTT+GGG - chr3_3:71014384-71014403 Msa0469220:CDS 30.0%
GTATGTGAATTAACCCATTT+AGG - chr3_3:71014494-71014513 Msa0469220:intron 30.0%
TCCATATAGTATGCTAATAG+TGG - chr3_3:71014740-71014759 Msa0469220:intron 30.0%
ACCACTATTAGCATACTATA+TGG + chr3_3:71014744-71014763 None:intergenic 30.0%
CAATGATTCCTTTGAACTTA+TGG - chr3_3:71014794-71014813 Msa0469220:intron 30.0%
GAACTTATGGTAAAATCATC+CGG - chr3_3:71014807-71014826 Msa0469220:intron 30.0%
GTTATATAAGTCCACAGATT+TGG + chr3_3:71014930-71014949 None:intergenic 30.0%
TGTTCATATACATAGTGTGT+TGG + chr3_3:71015014-71015033 None:intergenic 30.0%
CTAACTGCTCTATAAATGAT+CGG + chr3_3:71015157-71015176 None:intergenic 30.0%
ATGTAGTTGTAAAGAGAAGA+AGG - chr3_3:71015286-71015305 Msa0469220:intron 30.0%
! TATGTTGCTTAAACAAAGCT+GGG - chr3_3:71015320-71015339 Msa0469220:intron 30.0%
GGAACCAAATCATTTATTCA+TGG + chr3_3:71015475-71015494 None:intergenic 30.0%
! ACTTTACTGATTACTCACTT+TGG + chr3_3:71015499-71015518 None:intergenic 30.0%
CAGTAAAGTGAGAAAATCAA+TGG - chr3_3:71015510-71015529 Msa0469220:intron 30.0%
! AGCAAGTATCTGTTACTTAA+GGG + chr3_3:71015833-71015852 None:intergenic 30.0%
AAATACTTGTAGGGAGAAAT+GGG + chr3_3:71015874-71015893 None:intergenic 30.0%
ATTCTGTGATACTAACATAC+TGG + chr3_3:71015969-71015988 None:intergenic 30.0%
ACCCTGTTAATATGTACAAA+AGG - chr3_3:71016009-71016028 Msa0469220:intron 30.0%
!! GCTGATTATTTTTCTGTCTT+GGG - chr3_3:71016173-71016192 Msa0469220:intron 30.0%
TACTTCTGATCATTTCATCA+TGG - chr3_3:71016312-71016331 Msa0469220:intron 30.0%
!! AAAGAATTTGCTGAGAAAGA+AGG - chr3_3:71016615-71016634 Msa0469220:intron 30.0%
! AGGAACAATAATCTTTTTGC+CGG + chr3_3:71016787-71016806 None:intergenic 30.0%
ATGTAAATATACACGGGAAA+TGG + chr3_3:71016934-71016953 None:intergenic 30.0%
GTAATCAACCTCTCTATATT+AGG + chr3_3:71017110-71017129 None:intergenic 30.0%
AAATAGCCTAATATAGAGCA+TGG - chr3_3:71017160-71017179 Msa0469220:five_prime_UTR 30.0%
TAGTAACCATGCTCTATATT+AGG + chr3_3:71017169-71017188 None:intergenic 30.0%
!!! GTCTTCAAGGTGGTTTTAAT+CGG - chr3_3:71013730-71013749 Msa0469220:three_prime_UTR 35.0%
GAAAATCTCAGATACTAGCT+CGG - chr3_3:71013767-71013786 Msa0469220:three_prime_UTR 35.0%
TGAGTTTCAAACTCGTACTT+TGG - chr3_3:71013837-71013856 Msa0469220:three_prime_UTR 35.0%
! GTTTTTGCCTATGGTTTCTT+TGG - chr3_3:71014383-71014402 Msa0469220:CDS 35.0%
CGCAGGATTTAAGTAAGAAA+AGG - chr3_3:71014442-71014461 Msa0469220:intron 35.0%
! TGAATTAACCCATTTAGGGT+TGG - chr3_3:71014499-71014518 Msa0469220:intron 35.0%
!! CAGATTTCTGAAACTTACGA+GGG - chr3_3:71014596-71014615 Msa0469220:intron 35.0%
!! AGATTTCTGAAACTTACGAG+GGG - chr3_3:71014597-71014616 Msa0469220:intron 35.0%
CAGTTTGCATGCTCTTTAAT+CGG - chr3_3:71014668-71014687 Msa0469220:intron 35.0%
GTTCAAAGGAATCATTGCTA+AGG + chr3_3:71014791-71014810 None:intergenic 35.0%
ATTTCAATTGGCCAAATCTG+TGG - chr3_3:71014916-71014935 Msa0469220:intron 35.0%
!! TGTTGGTAGCATTTCTAACA+TGG + chr3_3:71014997-71015016 None:intergenic 35.0%
ATTTACTTGAATGACACGTG+TGG - chr3_3:71015102-71015121 Msa0469220:intron 35.0%
CGATCATTTATAGAGCAGTT+AGG - chr3_3:71015155-71015174 Msa0469220:intron 35.0%
! ACATTGATGGGAAATGGATT+TGG - chr3_3:71015181-71015200 Msa0469220:intron 35.0%
ATTTGGAAGTTGGGAGAAAA+TGG - chr3_3:71015198-71015217 Msa0469220:intron 35.0%
TTTGGAAGTTGGGAGAAAAT+GGG - chr3_3:71015199-71015218 Msa0469220:intron 35.0%
! GTATGTTGCTTAAACAAAGC+TGG - chr3_3:71015319-71015338 Msa0469220:intron 35.0%
! TTACTGAGTCTGAGATTTCA+GGG - chr3_3:71015414-71015433 Msa0469220:intron 35.0%
CAGCCCATGAATAAATGATT+TGG - chr3_3:71015468-71015487 Msa0469220:intron 35.0%
! TTACTGATTACTCACTTTGG+AGG + chr3_3:71015496-71015515 None:intergenic 35.0%
GCACATGCATGATAAATCAT+GGG + chr3_3:71015551-71015570 None:intergenic 35.0%
TGCACATGCATGATAAATCA+TGG + chr3_3:71015552-71015571 None:intergenic 35.0%
CTCTAATTTATTGCTGGATC+AGG + chr3_3:71015663-71015682 None:intergenic 35.0%
GCAAGACTCTAATTTATTGC+TGG + chr3_3:71015669-71015688 None:intergenic 35.0%
TTACTTAAGGGAGTTAGAGT+TGG + chr3_3:71015821-71015840 None:intergenic 35.0%
! GAGCAAGTATCTGTTACTTA+AGG + chr3_3:71015834-71015853 None:intergenic 35.0%
GAAATACTTGTAGGGAGAAA+TGG + chr3_3:71015875-71015894 None:intergenic 35.0%
CCAGAAAGGAAATACTTGTA+GGG + chr3_3:71015883-71015902 None:intergenic 35.0%
!! GGCTGATTATTTTTCTGTCT+TGG - chr3_3:71016172-71016191 Msa0469220:intron 35.0%
TCAACCAATGTTCTTCAATC+TGG - chr3_3:71016238-71016257 Msa0469220:intron 35.0%
AGTTACAAGTGCATACTACA+GGG - chr3_3:71016422-71016441 Msa0469220:intron 35.0%
TGACTATATTCTTGTCAGCA+TGG + chr3_3:71016533-71016552 None:intergenic 35.0%
AGGAAACAAAAGTGATCTTG+AGG - chr3_3:71016563-71016582 Msa0469220:intron 35.0%
! AATTCTTTTGCATCCTCTGT+GGG + chr3_3:71016603-71016622 None:intergenic 35.0%
! AAATTCTTTTGCATCCTCTG+TGG + chr3_3:71016604-71016623 None:intergenic 35.0%
TTCTGTCAAAACAGTCATGA+AGG + chr3_3:71016691-71016710 None:intergenic 35.0%
GGCAAAAAGATTATTGTTCC+TGG - chr3_3:71016786-71016805 Msa0469220:intron 35.0%
AGCATGTGTTGTCAGTAATA+CGG - chr3_3:71016834-71016853 Msa0469220:intron 35.0%
!! TTCGTATGACCAAGTTTTGA+TGG - chr3_3:71016903-71016922 Msa0469220:intron 35.0%
!! ATGACCAAGTTTTGATGGTT+TGG - chr3_3:71016908-71016927 Msa0469220:intron 35.0%
!! TGTTGATTTAAGACCTGAGA+TGG - chr3_3:71016976-71016995 Msa0469220:CDS 35.0%
ACAAGCGCTACTAATTTCAT+AGG - chr3_3:71017027-71017046 Msa0469220:CDS 35.0%
AATTTCATAGGTAGCCTGAT+TGG - chr3_3:71017039-71017058 Msa0469220:CDS 35.0%
ACAATGTTCCAATTCCAATC+AGG + chr3_3:71017056-71017075 None:intergenic 35.0%
CCTCTCTATATTAGGCTATT+TGG + chr3_3:71017102-71017121 None:intergenic 35.0%
CCAAATAGCCTAATATAGAG+AGG - chr3_3:71017099-71017118 Msa0469220:CDS 35.0%
TGAAGACGTAGTCTATCTTC+TGG + chr3_3:71013717-71013736 None:intergenic 40.0%
GAAGATAGACTACGTCTTCA+AGG - chr3_3:71013717-71013736 Msa0469220:three_prime_UTR 40.0%
!! TATCTGAGATTTTCCAACGG+CGG + chr3_3:71013761-71013780 None:intergenic 40.0%
GTGGACTCAAAAGCTACAAT+CGG - chr3_3:71013811-71013830 Msa0469220:three_prime_UTR 40.0%
AGAAAGAAGAGAACCGTTCT+TGG + chr3_3:71013909-71013928 None:intergenic 40.0%
ACTCATCCCAAAGAAACCAT+AGG + chr3_3:71014393-71014412 None:intergenic 40.0%
!! GCAGATTTCTGAAACTTACG+AGG - chr3_3:71014595-71014614 Msa0469220:intron 40.0%
TCGGCATATTTGAACTTCAG+TGG - chr3_3:71014687-71014706 Msa0469220:intron 40.0%
GTATGCATTCGACATGAATG+AGG + chr3_3:71014862-71014881 None:intergenic 40.0%
GTTGGATTTCATACGTCACA+AGG - chr3_3:71015124-71015143 Msa0469220:intron 40.0%
AGCAGTTAGGAAGACATTGA+TGG - chr3_3:71015168-71015187 Msa0469220:intron 40.0%
GCAGTTAGGAAGACATTGAT+GGG - chr3_3:71015169-71015188 Msa0469220:intron 40.0%
AGGAAGACATTGATGGGAAA+TGG - chr3_3:71015175-71015194 Msa0469220:intron 40.0%
! TGGGAAATGGATTTGGAAGT+TGG - chr3_3:71015188-71015207 Msa0469220:intron 40.0%
! GGGAAATGGATTTGGAAGTT+GGG - chr3_3:71015189-71015208 Msa0469220:intron 40.0%
GGTCAGAAAGTAAGAAGAAC+AGG - chr3_3:71015341-71015360 Msa0469220:intron 40.0%
! GTTACTGAGTCTGAGATTTC+AGG - chr3_3:71015413-71015432 Msa0469220:intron 40.0%
CATGCATGATAAATCATGGG+TGG + chr3_3:71015548-71015567 None:intergenic 40.0%
GTAGTTGTGTTTAGAGGGAA+AGG + chr3_3:71015691-71015710 None:intergenic 40.0%
TAGCGGTAGTTGTGTTTAGA+GGG + chr3_3:71015696-71015715 None:intergenic 40.0%
TTAGCGGTAGTTGTGTTTAG+AGG + chr3_3:71015697-71015716 None:intergenic 40.0%
CAATAGGGGATTCAGTTTAG+CGG + chr3_3:71015713-71015732 None:intergenic 40.0%
ATGTGTGGAGAGAACAATAG+GGG + chr3_3:71015727-71015746 None:intergenic 40.0%
GATGTGTGGAGAGAACAATA+GGG + chr3_3:71015728-71015747 None:intergenic 40.0%
AGGTAGGTTATAAGGATGTG+TGG + chr3_3:71015742-71015761 None:intergenic 40.0%
ATTGGCAGAGGTAGGTTATA+AGG + chr3_3:71015750-71015769 None:intergenic 40.0%
CCCTACAAGTATTTCCTTTC+TGG - chr3_3:71015880-71015899 Msa0469220:intron 40.0%
GCCAGAAAGGAAATACTTGT+AGG + chr3_3:71015884-71015903 None:intergenic 40.0%
CTCACCAGATTGAAGAACAT+TGG + chr3_3:71016245-71016264 None:intergenic 40.0%
CAGTTACAAGTGCATACTAC+AGG - chr3_3:71016421-71016440 Msa0469220:intron 40.0%
GTTACAAGTGCATACTACAG+GGG - chr3_3:71016423-71016442 Msa0469220:intron 40.0%
GCAAGCAACTAATGTTGAGA+CGG - chr3_3:71016665-71016684 Msa0469220:intron 40.0%
CATTGTCAACAAGAAGAACC+TGG - chr3_3:71016719-71016738 Msa0469220:intron 40.0%
TTCTTAGGGATTTCTTGTGC+AGG + chr3_3:71016813-71016832 None:intergenic 40.0%
GACAACACATGCTCTTCTTA+GGG + chr3_3:71016827-71016846 None:intergenic 40.0%
TGACAACACATGCTCTTCTT+AGG + chr3_3:71016828-71016847 None:intergenic 40.0%
GTCATACGAACATAGAACCA+TGG + chr3_3:71016893-71016912 None:intergenic 40.0%
ATGGCCAAACCATCAAAACT+TGG + chr3_3:71016915-71016934 None:intergenic 40.0%
! TTTAAGACCTGAGATGGAGT+CGG - chr3_3:71016982-71017001 Msa0469220:CDS 40.0%
ATAGGTAGCCTGATTGGAAT+TGG - chr3_3:71017045-71017064 Msa0469220:CDS 40.0%
! AGAGAGGTTGATTACTGCTA+TGG - chr3_3:71017115-71017134 Msa0469220:CDS 40.0%
! GATTTTTACCACGAAGTGTG+TGG - chr3_3:71017203-71017222 Msa0469220:five_prime_UTR 40.0%
! AATGTTTTCCACACACTTCG+TGG + chr3_3:71017214-71017233 None:intergenic 40.0%
GATAGACTACGTCTTCAAGG+TGG - chr3_3:71013720-71013739 Msa0469220:three_prime_UTR 45.0%
! AGAGTGTTAAGGCTCAGATC+TGG - chr3_3:71013872-71013891 Msa0469220:three_prime_UTR 45.0%
GAGTGTTAAGGCTCAGATCT+GGG - chr3_3:71013873-71013892 Msa0469220:three_prime_UTR 45.0%
!! GTTTGACTGATGTTGACTCG+AGG + chr3_3:71014310-71014329 None:intergenic 45.0%
!! GTTTGACTGATGTTGACTCG+AGG - chr3_3:71014310-71014329 Msa0469220:CDS 45.0%
AGTTTGAGTGTGTATGAGCG+AGG - chr3_3:71014410-71014429 Msa0469220:CDS 45.0%
!! TAACCCATTTAGGGTTGGCT+TGG - chr3_3:71014504-71014523 Msa0469220:intron 45.0%
!! AACCCATTTAGGGTTGGCTT+GGG - chr3_3:71014505-71014524 Msa0469220:intron 45.0%
AACCCAAGCCAACCCTAAAT+GGG + chr3_3:71014510-71014529 None:intergenic 45.0%
CATACTGCCTGACTTAACTC+AGG - chr3_3:71014877-71014896 Msa0469220:intron 45.0%
CATGTCACCTGAGTTAAGTC+AGG + chr3_3:71014887-71014906 None:intergenic 45.0%
ACTTGAATGACACGTGTGGT+TGG - chr3_3:71015106-71015125 Msa0469220:intron 45.0%
CTCAGTAACTCCTAGCTGTT+GGG + chr3_3:71015402-71015421 None:intergenic 45.0%
ACTCAGTAACTCCTAGCTGT+TGG + chr3_3:71015403-71015422 None:intergenic 45.0%
TGTGAGCAACTCACTGTAGA+GGG + chr3_3:71015631-71015650 None:intergenic 45.0%
TTGTGAGCAACTCACTGTAG+AGG + chr3_3:71015632-71015651 None:intergenic 45.0%
CAGTGAGTTGCTCACAATAG+AGG - chr3_3:71015635-71015654 Msa0469220:intron 45.0%
GGATGTGTGGAGAGAACAAT+AGG + chr3_3:71015729-71015748 None:intergenic 45.0%
AGATAGCACTCTCACAGGAT+TGG + chr3_3:71015768-71015787 None:intergenic 45.0%
!! AATCCTGTGAGAGTGCTATC+TGG - chr3_3:71015767-71015786 Msa0469220:intron 45.0%
GTAACAGATACTTGCTCAGC+TGG - chr3_3:71015837-71015856 Msa0469220:intron 45.0%
GCTGTGTGCTTACAGACATT+GGG - chr3_3:71015902-71015921 Msa0469220:intron 45.0%
GGTGCATGAGTTGTACACTA+AGG + chr3_3:71015992-71016011 None:intergenic 45.0%
CTAGGTATGTGATCGAAGCT+CGG + chr3_3:71016489-71016508 None:intergenic 45.0%
GCTTCGATCACATACCTAGA+TGG - chr3_3:71016490-71016509 Msa0469220:intron 45.0%
CGCAGTTCTTCTAACCATCT+AGG + chr3_3:71016507-71016526 None:intergenic 45.0%
GCTGATGAGAATCAGGGAAA+TGG - chr3_3:71016744-71016763 Msa0469220:intron 45.0%
GGTTGATTACTGCTATGGTG+TGG - chr3_3:71017120-71017139 Msa0469220:CDS 45.0%
ACAATGGCGAGTGCAGGTTA+CGG - chr3_3:71013682-71013701 Msa0469220:three_prime_UTR 50.0%
TTAATCGGTGATTCCGCCGT+TGG - chr3_3:71013745-71013764 Msa0469220:three_prime_UTR 50.0%
TGCAAGAAACGACTTCAGCG+TGG - chr3_3:71013792-71013811 Msa0469220:three_prime_UTR 50.0%
!! GACTGATGTTGACTCGAGGT+TGG - chr3_3:71014314-71014333 Msa0469220:CDS 50.0%
GAACCCAAGCCAACCCTAAA+TGG + chr3_3:71014511-71014530 None:intergenic 50.0%
!! TTAGGGTTGGCTTGGGTTCT+TGG - chr3_3:71014512-71014531 Msa0469220:intron 50.0%
AGCGATACAGACTGCAGAGT+AGG + chr3_3:71015255-71015274 None:intergenic 50.0%
CATAACTCCTCCCAACAGCT+AGG - chr3_3:71015389-71015408 Msa0469220:intron 50.0%
AGTAACTCCTAGCTGTTGGG+AGG + chr3_3:71015399-71015418 None:intergenic 50.0%
AGACCAGATAGCACTCTCAC+AGG + chr3_3:71015773-71015792 None:intergenic 50.0%
GGGAGAAATGGGTGAGTGTT+TGG + chr3_3:71015863-71015882 None:intergenic 50.0%
CTGTAAGCACACAGCCAGAA+AGG + chr3_3:71015897-71015916 None:intergenic 50.0%
GGCTGTGTGCTTACAGACAT+TGG - chr3_3:71015901-71015920 Msa0469220:intron 50.0%
ATTAGTTGCTTGCAGCGCAG+AGG + chr3_3:71016658-71016677 None:intergenic 50.0%
CTGGCTGCTGATGAGAATCA+GGG - chr3_3:71016738-71016757 Msa0469220:intron 50.0%
ATTACCTCCGACTCCATCTC+AGG + chr3_3:71016992-71017011 None:intergenic 50.0%
GCAAGAACAATGGCGAGTGC+AGG - chr3_3:71013676-71013695 Msa0469220:three_prime_UTR 55.0%
! CTCACAGGATTGGCAGAGGT+AGG + chr3_3:71015758-71015777 None:intergenic 55.0%
! CACTCTCACAGGATTGGCAG+AGG + chr3_3:71015762-71015781 None:intergenic 55.0%
!! CATACTACAGGGGCGCTGTT+GGG - chr3_3:71016433-71016452 Msa0469220:intron 55.0%
!! ATACTACAGGGGCGCTGTTG+GGG - chr3_3:71016434-71016453 Msa0469220:intron 55.0%
GTGATCGAAGCTCGGACGTT+TGG + chr3_3:71016481-71016500 None:intergenic 55.0%
CCTGATTCTCATCAGCAGCC+AGG + chr3_3:71016740-71016759 None:intergenic 55.0%
CCTGGCTGCTGATGAGAATC+AGG - chr3_3:71016737-71016756 Msa0469220:intron 55.0%
GGCAACTCAGCATCTCTGTC+CGG - chr3_3:71016765-71016784 Msa0469220:intron 55.0%
GGGATTTCTTGTGCAGGACC+AGG + chr3_3:71016807-71016826 None:intergenic 55.0%
AAGACCTGAGATGGAGTCGG+AGG - chr3_3:71016985-71017004 Msa0469220:CDS 55.0%
! GCTCAGATCTGGGACACTGC+TGG - chr3_3:71013883-71013902 Msa0469220:three_prime_UTR 60.0%
GGGACACTGCTGGCCAAGAA+CGG - chr3_3:71013893-71013912 Msa0469220:three_prime_UTR 60.0%
GAGCGAGGTGTGATTGACGC+AGG - chr3_3:71014425-71014444 Msa0469220:intron 60.0%
!! GCATACTACAGGGGCGCTGT+TGG - chr3_3:71016432-71016451 Msa0469220:intron 60.0%
! GGGCGCTGTTGGGGCAATGT+TGG - chr3_3:71016443-71016462 Msa0469220:intron 65.0%
GCAGCGTGCAGTACCCACAG+AGG - chr3_3:71016587-71016606 Msa0469220:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 71013600 71017242 71013600 ID=Msa0469220;Name=Msa0469220
chr3_3 mRNA 71013600 71017242 71013600 ID=Msa0469220-mRNA-1;Parent=Msa0469220;Name=Msa0469220-mRNA-1;_AED=0.16;_eAED=0.16;_QI=85|1|1|1|1|1|2|391|222
chr3_3 exon 71013600 71014429 71013600 ID=Msa0469220-mRNA-1:exon:17437;Parent=Msa0469220-mRNA-1
chr3_3 exon 71016928 71017242 71016928 ID=Msa0469220-mRNA-1:exon:17436;Parent=Msa0469220-mRNA-1
chr3_3 five_prime_UTR 71017158 71017242 71017158 ID=Msa0469220-mRNA-1:five_prime_utr;Parent=Msa0469220-mRNA-1
chr3_3 CDS 71016928 71017157 71016928 ID=Msa0469220-mRNA-1:cds;Parent=Msa0469220-mRNA-1
chr3_3 CDS 71013991 71014429 71013991 ID=Msa0469220-mRNA-1:cds;Parent=Msa0469220-mRNA-1
chr3_3 three_prime_UTR 71013600 71013990 71013600 ID=Msa0469220-mRNA-1:three_prime_utr;Parent=Msa0469220-mRNA-1
Gene Sequence

>Msa0469220

ATGGCGAGTGCAGGTTACGGTGATGCAAACCAGAAGATAGACTACGTCTTCAAGGTGGTTTTAATCGGTGATTCCGCCGTTGGAAAATCTCAGATACTAGCTCGGTTTGCAAGAAACGACTTCAGCGTGGACTCAAAAGCTACAATCGGTGTTGAGTTTCAAACTCGTACTTTGGTTATTGAACATAAGAGTGTTAAGGCTCAGATCTGGGACACTGCTGGCCAAGAACGATATAGAGCAGTTACAAGTGCATACTACAGGGGCGCTGTTGGGGCAATGTTGGTTTATGACATTACCAAACGTCCGAGCTTCGATCACATACCTAGATGGTTAGAAGAACTGCGCAACCATGCTGACAAGAATATAGTCATCATTCTTATAGGAAACAAAAGTGATCTTGAGGAGCAGCGTGCAGTACCCACAGAGGATGCAAAAGAATTTGCTGAGAAAGAAGGCTTATTTTTCTTAGAGACCTCTGCGCTGCAAGCAACTAATGTTGAGACGGCCTTCATGACTGTTTTGACAGAAATATTTAACATTGTCAACAAGAAGAACCTGGCTGCTGATGAGAATCAGGGAAATGGCAACTCAGCATCTCTGTCCGGCAAAAAGATTATTGTTCCTGGTCCTGCACAAGAAATCCCTAAGAAGAGCATGTGTTGTCAGTAA
Protein sequence

>Msa0469220

MASAGYGDANQKIDYVFKVVLIGDSAVGKSQILARFARNDFSVDSKATIGVEFQTRTLVIEHKSVKAQIWDTAGQERYRAVTSAYYRGAVGAMLVYDITKRPSFDHIPRWLEELRNHADKNIVIILIGNKSDLEEQRAVPTEDAKEFAEKEGLFFLETSALQATNVETAFMTVLTEIFNIVNKKNLAADENQGNGNSASLSGKKIIVPGPAQEIPKKSMCCQ*