AlfalfaGEDB | Msa0483240

M. sativa cultivar zhongmu-4 / Msa0483240

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0099402
PFAM: PF08880.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0483240	XP_003627270.3	96.243	346	8	2	1	341	1	346	0.0	685

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0483240	sp\|Q8L8A6\|GRF5_ARATH	41.033	368	165	10	3	321	8	372	2.72e-70	227

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0483240	A0A396GLB2	96.243	346	8	2	1	341	1	346	0.0	685

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TFs/TRs:

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Gene ID	Type	Classification
Msa0483240	TF	GRF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0483240	Msa1191520	0.856791	2.533568e-62	-8.615850e-47
Msa0483240	Msa1234140	0.832969	6.976598e-56	-8.615850e-47
Msa0483240	Msa1276370	0.873728	1.174495e-67	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0483240	MtrunA17_Chr8g0343881	96.243	346	8	2	1	341	1	346	0.0	685
Msa0483240	MtrunA17_Chr4g0070591	44.444	360	150	17	1	333	1	337	2.13e-75	236
Msa0483240	MtrunA17_Chr2g0300511	42.165	351	159	12	7	337	3	329	3.23e-69	220
Msa0483240	MtrunA17_Chr4g0021261	38.415	328	147	12	10	333	24	300	2.07e-55	184
Msa0483240	MtrunA17_Chr1g0152191	52.846	123	54	1	1	119	134	256	1.20e-40	150
Msa0483240	MtrunA17_Chr5g0409471	58.333	108	33	2	12	119	17	112	1.92e-39	142
Msa0483240	MtrunA17_Chr7g0265931	45.562	169	81	5	12	177	79	239	9.73e-36	133
Msa0483240	MtrunA17_Chr3g0127641	47.706	109	39	2	12	120	113	203	2.18e-28	115
Msa0483240	MtrunA17_Chr8g0351521	36.424	151	79	2	8	158	51	184	5.29e-21	93.2
Msa0483240	MtrunA17_Chr8g0351521	52.727	55	26	0	75	129	302	356	1.06e-12	68.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0483240	AT3G13960.1	41.033	368	165	10	3	321	8	372	2.76e-71	227
Msa0483240	AT2G06200.2	58.915	129	38	3	2	117	36	162	3.55e-43	150
Msa0483240	AT2G06200.1	59.836	122	34	3	9	117	4	123	6.13e-43	149
Msa0483240	AT4G37740.1	50.000	152	55	4	12	145	165	313	2.92e-41	151
Msa0483240	AT2G22840.1	57.018	114	35	4	12	119	134	239	3.53e-35	134
Msa0483240	AT2G36400.1	51.181	127	47	4	10	127	75	195	8.67e-35	131
Msa0483240	AT3G52910.1	50.420	119	43	3	11	119	82	194	8.09e-32	123
Msa0483240	AT2G06200.3	88.235	51	4	1	69	117	1	51	2.88e-25	100
Msa0483240	AT5G53660.1	47.222	108	40	1	12	119	60	150	8.72e-25	103
Msa0483240	AT4G24150.3	41.739	115	61	2	10	119	110	223	1.93e-24	103

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Find 63 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCACTTCATCATTTCTT+TGG	0.279787	3_3:+91122585	Msa0483240:CDS
AAACCGTTCAAGAAAGCCTG+TGG	0.653157	3_3:+91121722	Msa0483240:CDS
AAGTAGACCCAGAGCCAGGG+AGG	0.659120	3_3:+91121610	Msa0483240:CDS
ACCTGTTGGAACTCTGGAGT+TGG	0.501428	3_3:-91122652	None:intergenic
ACTTTCTTTCCAGAAGCTAC+AGG	0.451806	3_3:+91122363	Msa0483240:CDS
AGAAAAGTAGACCCAGAGCC+AGG	0.407864	3_3:+91121606	Msa0483240:CDS
AGCCAGGAATCTGTGTTCTT+TGG	0.341985	3_3:-91122618	None:intergenic
AGGAATAAGGGATGGTGCTG+TGG	0.601471	3_3:+91122332	Msa0483240:CDS
ATAAATGATAATCAACCTGT+TGG	0.523980	3_3:-91122666	None:intergenic
ATAGAGTATATGAGATCTGG+TGG	0.579210	3_3:-91121375	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTATTTAAGAGGGTTAAGAG+GGG	-	chr3_3:91121186-91121205	None:intergenic	30.0%
ATCTTATTCTCATGATGACA+AGG	+	chr3_3:91122031-91122050	Msa0483240:CDS	30.0%
GTATTTAGAGGTCTCTATAA+CGG	-	chr3_3:91122173-91122192	None:intergenic	30.0%
AAACCACTTCATCATTTCTT+TGG	+	chr3_3:91122585-91122604	Msa0483240:CDS	30.0%
TTATTTAAGAGGGTTAAGAG+GGG	-	chr3_3:91121186-91121205	None:intergenic	30.0%
ATCTTATTCTCATGATGACA+AGG	+	chr3_3:91122031-91122050	Msa0483240:CDS	30.0%
GTATTTAGAGGTCTCTATAA+CGG	-	chr3_3:91122173-91122192	None:intergenic	30.0%
AAACCACTTCATCATTTCTT+TGG	+	chr3_3:91122585-91122604	Msa0483240:CDS	30.0%
ATTATTGTACTGTGCATGGA+TGG	-	chr3_3:91121157-91121176	None:intergenic	35.0%
TGAGTGCAAGTTCAAGAAAT+AGG	+	chr3_3:91121271-91121290	Msa0483240:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	91121118	91122677	91121118	ID=Msa0483240;Name=Msa0483240
chr3_3	mRNA	91121118	91122677	91121118	ID=Msa0483240-mRNA-1;Parent=Msa0483240;Name=Msa0483240-mRNA-1;_AED=0.10;_eAED=0.10;_QI=149\|1\|0.33\|1\|1\|1\|3\|0\|342
chr3_3	exon	91121118	91121450	91121118	ID=Msa0483240-mRNA-1:exon:26616;Parent=Msa0483240-mRNA-1
chr3_3	exon	91121574	91122060	91121574	ID=Msa0483240-mRNA-1:exon:26617;Parent=Msa0483240-mRNA-1
chr3_3	exon	91122320	91122677	91122320	ID=Msa0483240-mRNA-1:exon:26618;Parent=Msa0483240-mRNA-1
chr3_3	five_prime_UTR	91121118	91121266	91121118	ID=Msa0483240-mRNA-1:five_prime_utr;Parent=Msa0483240-mRNA-1
chr3_3	CDS	91121267	91121450	91121267	ID=Msa0483240-mRNA-1:cds;Parent=Msa0483240-mRNA-1
chr3_3	CDS	91121574	91122060	91121574	ID=Msa0483240-mRNA-1:cds;Parent=Msa0483240-mRNA-1
chr3_3	CDS	91122320	91122677	91122320	ID=Msa0483240-mRNA-1:cds;Parent=Msa0483240-mRNA-1

Gene Sequence

>Msa0483240

ATGATGAGTGCAAGTTCAAGAAATAGGTCTCTTTTCACACCAAATCAATGGCAAGAACTTGAACAACAAGCCCTAGTTTTTAAATACATGGTTACTGGAACACCTATTCCACCAGATCTCATATACTCTATTAAGAGAAGTTTAGACACTTCAATATCTTCAAGAATCTTTCCTCATCCACCAATTGGGTGGGGATGTTTTGAAATGGGATTTGGCAGAAAAGTAGACCCAGAGCCAGGGAGGTGCAGAAGAACAGATGGCAAGAAATGGAGATGCTCAAAGGAAGCATATCCAGACTCAAAGTACTGTGAAAGACACATGCACAGAGGTAGAAACCGTTCAAGAAAGCCTGTGGAACTAGTAGTTTCTTCTTCAACAACAACACCAACAAATAACACAAACACAGCATCTTCTTACAACAGAAACACCTCCTTGAACAACAACAGCAGCAGCATAAACTCACCTTCTTCTTTCCCTTTCTCTACTTCTTCCATGGCTTGTCATGATCAGTCACAATCTTTTTCACAATCCTACCAAAACCCTTACTATTACTCTCAATCAATTACCTCTTCTAAAACACTTGATCATTCTGATTTCCAAACTCAAGATGCTACTACTCATCACCTCTTTTTGGACTCAACATCTTATTCTCATGATGACAAGGACTATAGGAGGCAAGTTCAAGGAATAAGGGATGGTGCTGTGGATGAGAGAACTTTCTTTCCAGAAGCTACAGGTTCATCTAGGAGCTGCTATCATGATTCATATCAACAACAACTATCAATGAATCCCTTTAAGTCTTACTCAAGCTCACAGTTTCAAAATATCAATGATGATAATTCAAGACAACAACAAGAACAACACTGTTTTGTTTTAGGCACTGACATCAAGTCAACAAGAACAACAAACAAGGACAAAGAAAGTGAGACAACTCAGAAACCACTTCATCATTTCTTTGGTGAGTGGACACCAAAGAACACAGATTCCTGGCTAGATCTTGCTTCCAACTCCAGAGTTCCAACAGGTTGA

Protein sequence

>Msa0483240

MMSASSRNRSLFTPNQWQELEQQALVFKYMVTGTPIPPDLIYSIKRSLDTSISSRIFPHPPIGWGCFEMGFGRKVDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVELVVSSSTTTPTNNTNTASSYNRNTSLNNNSSSINSPSSFPFSTSSMACHDQSQSFSQSYQNPYYYSQSITSSKTLDHSDFQTQDATTHHLFLDSTSYSHDDKDYRRQVQGIRDGAVDERTFFPEATGSSRSCYHDSYQQQLSMNPFKSYSSSQFQNINDDNSRQQQEQHCFVLGTDIKSTRTTNKDKESETTQKPLHHFFGEWTPKNTDSWLDLASNSRVPTG*