AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1276370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1276370 MtrunA17_Chr8g0343881 94.540 348 10 3 1 339 1 348 0.0 673
Msa1276370 MtrunA17_Chr4g0070591 43.575 358 152 17 1 329 1 337 3.85e-73 230
Msa1276370 MtrunA17_Chr2g0300511 41.860 344 166 11 7 333 3 329 5.84e-70 222
Msa1276370 MtrunA17_Chr4g0021261 38.532 327 144 12 10 329 24 300 2.31e-54 181
Msa1276370 MtrunA17_Chr1g0152191 52.846 123 54 1 1 119 134 256 1.30e-40 150
Msa1276370 MtrunA17_Chr5g0409471 58.333 108 33 2 12 119 17 112 1.86e-39 142
Msa1276370 MtrunA17_Chr7g0265931 54.472 123 46 4 12 132 79 193 8.89e-36 133
Msa1276370 MtrunA17_Chr3g0127641 47.706 109 39 2 12 120 113 203 2.44e-28 115
Msa1276370 MtrunA17_Chr8g0351521 36.424 151 79 2 8 158 51 184 7.55e-21 92.8
Msa1276370 MtrunA17_Chr8g0351521 52.727 55 26 0 75 129 302 356 8.90e-13 68.9
Msa1276370 MtrunA17_Chr8g0351291 64.103 39 14 0 75 113 51 89 1.16e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1276370 AT3G13960.1 40.379 369 162 10 3 317 8 372 1.17e-70 225
Msa1276370 AT2G06200.2 58.915 129 38 3 2 117 36 162 3.66e-43 150
Msa1276370 AT2G06200.1 59.836 122 34 3 9 117 4 123 6.39e-43 149
Msa1276370 AT4G37740.1 49.342 152 56 4 12 145 165 313 1.23e-40 149
Msa1276370 AT2G22840.1 57.018 114 35 4 12 119 134 239 3.68e-35 134
Msa1276370 AT2G36400.1 53.782 119 40 4 10 119 75 187 8.09e-35 131
Msa1276370 AT3G52910.1 40.704 199 95 6 11 195 82 271 1.64e-32 125
Msa1276370 AT2G06200.3 88.235 51 4 1 69 117 1 51 2.60e-25 100
Msa1276370 AT5G53660.1 47.222 108 40 1 12 119 60 150 8.53e-25 103
Msa1276370 AT4G24150.3 41.739 115 61 2 10 119 110 223 2.18e-24 103
Msa1276370 AT4G24150.2 41.739 115 61 2 10 119 148 261 3.90e-24 102
Msa1276370 AT4G24150.1 37.143 140 57 3 10 119 148 286 4.76e-22 97.1
Msa1276370 AT2G45480.2 46.535 101 54 0 16 116 29 129 5.82e-16 77.8
Msa1276370 AT2G45480.3 37.419 155 90 2 16 163 29 183 1.77e-15 77.4
Msa1276370 AT2G45480.3 47.500 80 31 3 40 119 281 349 2.34e-14 73.9
Msa1276370 AT2G45480.1 46.535 101 54 0 16 116 27 127 1.94e-15 77.4
Msa1276370 AT2G45480.1 47.500 80 31 3 40 119 279 347 2.34e-14 73.9

Find 70 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGCAAGTTCAAGGAATA+AGG 0.269692 8_3:+10971041 Msa1276370:intron
CTTCTGCACCTCCCTGGCTC+TGG 0.278943 8_3:-10970368 None:intergenic
AAACCACTTCATCATTTCTT+TGG 0.279787 8_3:+10971307 Msa1276370:CDS
AGCCAGGAATCTGTGTTCTT+TGG 0.341985 8_3:-10971340 None:intergenic
CTTTCACAGTACTTTGAGTC+TGG 0.352106 8_3:-10970431 None:intergenic
CTAGATGAACCTGTAGCTTC+TGG 0.361439 8_3:-10971094 None:intergenic
CCCTTTCTCTACTTCTTCCA+TGG 0.361986 8_3:+10970613 Msa1276370:CDS
TATTTGATTAAGAGACTTGC+TGG 0.365421 8_3:+10971588 Msa1276370:three_prime_UTR
GCTGTGTTTGTGTTATTTGT+TGG 0.366262 8_3:-10970524 None:intergenic
TTCTGCACCTCCCTGGCTCT+GGG 0.368474 8_3:-10970367 None:intergenic
GAGAGTAATAGTAAGGGTTT+TGG 0.368535 8_3:-10970673 None:intergenic
GCTGCTGTTGTTGTTGTTCA+AGG 0.381104 8_3:-10970570 None:intergenic
TTAAGAGGGTTAAGAGGGGT+TGG 0.387463 8_3:-10969929 None:intergenic
GTTATGTAGGGGACCACATT+AGG 0.395401 8_3:+10971696 Msa1276370:three_prime_UTR
TGATCTTTATTGTGTTATGT+AGG 0.395473 8_3:+10971683 Msa1276370:three_prime_UTR
TATATGAGATCTGGTGGAAT+AGG 0.406485 8_3:-10970119 None:intergenic
AGAAAAGTAGACCCAGAGCC+AGG 0.407864 8_3:+10970356 Msa1276370:CDS
TCAAGTTCTTGCCATTGATT+TGG 0.410002 8_3:-10970056 None:intergenic
ATTGATTGAGAGTAATAGTA+AGG 0.410096 8_3:-10970680 None:intergenic
GAGCTTGAGTAAGACTTAAA+GGG 0.410732 8_3:-10971160 None:intergenic
GAAGAACAGATGGCAAGAAA+TGG 0.429768 8_3:+10970387 Msa1276370:CDS
ATCAAAATGTTATGTGTGGC+TGG 0.435666 8_3:+10971635 Msa1276370:three_prime_UTR
CAGAAGCTACAGGTTCATCT+AGG 0.438969 8_3:+10971095 Msa1276370:CDS
GATCTTTATTGTGTTATGTA+GGG 0.443907 8_3:+10971684 Msa1276370:three_prime_UTR
TGAGTGCAAGTTCAAGAAAT+AGG 0.444135 8_3:+10970021 Msa1276370:CDS
ACTTTCTTTCCAGAAGCTAC+AGG 0.451806 8_3:+10971085 Msa1276370:CDS
TCTGTTCTTCTGCACCTCCC+TGG 0.454328 8_3:-10970374 None:intergenic
TTTATTTAAGAGGGTTAAGA+GGG 0.462557 8_3:-10969934 None:intergenic
TTAATAGAGTATATGAGATC+TGG 0.465803 8_3:-10970128 None:intergenic
TAATCAACCTGTTGGAACTC+TGG 0.470845 8_3:-10971380 None:intergenic
CACCAAAGAACACAGATTCC+TGG 0.474579 8_3:+10971338 Msa1276370:CDS
CTCATGATGACAAGGACTAT+AGG 0.477649 8_3:+10970789 Msa1276370:CDS
CAATACTTACTTGGTGGATG+AGG 0.492982 8_3:-10970188 None:intergenic
GCCATGGAAGAAGTAGAGAA+AGG 0.495080 8_3:-10970614 None:intergenic
ACCTGTTGGAACTCTGGAGT+TGG 0.501428 8_3:-10971374 None:intergenic
TCCAACTCCAGAGTTCCAAC+AGG 0.510080 8_3:+10971373 Msa1276370:CDS
GTAGAGAAAGGGAAAGAAGA+AGG 0.511856 8_3:-10970602 None:intergenic
CCATGGAAGAAGTAGAGAAA+GGG 0.516371 8_3:-10970613 None:intergenic
TGATAAAGGAGGCAAGTTCA+AGG 0.519079 8_3:+10971034 Msa1276370:intron
GAGGCAAGTTCAAGGAATAA+GGG 0.523074 8_3:+10971042 Msa1276370:CDS
GTTCCACAGGCTTTCTTGAA+CGG 0.529120 8_3:-10970475 None:intergenic
ATCTTTATTGTGTTATGTAG+GGG 0.533482 8_3:+10971685 Msa1276370:three_prime_UTR
TGTGAATGGTGCAGTTGGGT+GGG 0.535441 8_3:+10970310 Msa1276370:intron
TTCATCATTTCTTTGGTGAG+TGG 0.536164 8_3:+10971314 Msa1276370:CDS
GTTGGAAGCAAGATCTAGCC+AGG 0.536185 8_3:-10971356 None:intergenic
GTGAATGGTGCAGTTGGGTG+GGG 0.550789 8_3:+10970311 Msa1276370:intron
GAAAAGTAGACCCAGAGCCA+GGG 0.561252 8_3:+10970357 Msa1276370:CDS
ATTATTGTACTGTGCATGGA+TGG 0.567364 8_3:-10969904 None:intergenic
TCACCAAAGAAATGATGAAG+TGG 0.571081 8_3:-10971310 None:intergenic
TCAAAATGTTATGTGTGGCT+GGG 0.576383 8_3:+10971636 Msa1276370:three_prime_UTR
CTGTGAATGGTGCAGTTGGG+TGG 0.577092 8_3:+10970309 Msa1276370:intron
GGAGATTATTGTACTGTGCA+TGG 0.577269 8_3:-10969908 None:intergenic
ATAGAGTATATGAGATCTGG+TGG 0.579210 8_3:-10970125 None:intergenic
GAAGAAACTACTAGTTCCAC+AGG 0.590035 8_3:-10970488 None:intergenic
CAAGAAATGGAGATGCTCAA+AGG 0.592887 8_3:+10970400 Msa1276370:CDS
TTGATTGAGAGTAATAGTAA+GGG 0.594306 8_3:-10970679 None:intergenic
GGGAGGTGCAGAAGAACAGA+TGG 0.600412 8_3:+10970377 Msa1276370:CDS
ATCTTATTCTCATGATGACA+AGG 0.605341 8_3:+10970781 Msa1276370:CDS
TGACTGATCATGACAAGCCA+TGG 0.611257 8_3:-10970630 None:intergenic
CAAAATGTTATGTGTGGCTG+GGG 0.614659 8_3:+10971637 Msa1276370:three_prime_UTR
AGGAATAAGGGATGGTACTG+TGG 0.625865 8_3:+10971054 Msa1276370:CDS
TTGATTAAGAGACTTGCTGG+TGG 0.637573 8_3:+10971591 Msa1276370:three_prime_UTR
CAAGTTCAAGGAATAAGGGA+TGG 0.642231 8_3:+10971046 Msa1276370:CDS
GCTGTTGTTGTTGTTCAAGG+AGG 0.650068 8_3:-10970567 None:intergenic
AAACCGTTCAAGAAAGCCTG+TGG 0.653157 8_3:+10970472 Msa1276370:CDS
AAGTAGACCCAGAGCCAGGG+AGG 0.659120 8_3:+10970360 Msa1276370:CDS
GTCAACAAGAACAACAAACA+AGG 0.662924 8_3:+10971261 Msa1276370:CDS
TTATTTAAGAGGGTTAAGAG+GGG 0.668991 8_3:-10969933 None:intergenic
AAAGATCAAAATGTTATGTG+TGG 0.684063 8_3:+10971631 Msa1276370:three_prime_UTR
TGTGAAAGACACATGCACAG+AGG 0.772839 8_3:+10970446 Msa1276370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATATTTATATCAAATG+CGG + chr8_3:10970877-10970896 Msa1276370:intron 10.0%
!!! TTTTATATTTATATCAAATG+CGG + chr8_3:10970877-10970896 Msa1276370:intron 10.0%
!!! TGATTTTAAATTGTATTTAG+AGG - chr8_3:10970935-10970954 None:intergenic 15.0%
!! TTTATATTCTTTATTATCCA+TGG + chr8_3:10971548-10971567 Msa1276370:intron 15.0%
!!! TGATTTTAAATTGTATTTAG+AGG - chr8_3:10970935-10970954 None:intergenic 15.0%
!! TTTATATTCTTTATTATCCA+TGG + chr8_3:10971548-10971567 Msa1276370:intron 15.0%
!!! AAAAATAAGGATTTTCTTGA+TGG - chr8_3:10969985-10970004 None:intergenic 20.0%
!!! TTTTTTGAGACACAAAAATA+AGG - chr8_3:10969998-10970017 None:intergenic 20.0%
!! AACCATGTATTTAAAAACTA+GGG - chr8_3:10970090-10970109 None:intergenic 20.0%
!! TAACCATGTATTTAAAAACT+AGG - chr8_3:10970091-10970110 None:intergenic 20.0%
!! ACCAATAAATATATATACCA+TGG + chr8_3:10970822-10970841 Msa1276370:intron 20.0%
!! ACCATGGTATATATATTTAT+TGG - chr8_3:10970826-10970845 None:intergenic 20.0%
!! GAACAAAAAAACAAAAACAT+AGG - chr8_3:10971418-10971437 None:intergenic 20.0%
!!! AAAAATAAGGATTTTCTTGA+TGG - chr8_3:10969985-10970004 None:intergenic 20.0%
!!! TTTTTTGAGACACAAAAATA+AGG - chr8_3:10969998-10970017 None:intergenic 20.0%
!! AACCATGTATTTAAAAACTA+GGG - chr8_3:10970090-10970109 None:intergenic 20.0%
!! TAACCATGTATTTAAAAACT+AGG - chr8_3:10970091-10970110 None:intergenic 20.0%
!! ACCAATAAATATATATACCA+TGG + chr8_3:10970822-10970841 Msa1276370:intron 20.0%
!! ACCATGGTATATATATTTAT+TGG - chr8_3:10970826-10970845 None:intergenic 20.0%
!! GAACAAAAAAACAAAAACAT+AGG - chr8_3:10971418-10971437 None:intergenic 20.0%
! TTTATTTAAGAGGGTTAAGA+GGG - chr8_3:10969937-10969956 None:intergenic 25.0%
!!! TTTTATTTAAGAGGGTTAAG+AGG - chr8_3:10969938-10969957 None:intergenic 25.0%
!!! GAAGATGCTTTTATTTAAGA+GGG - chr8_3:10969946-10969965 None:intergenic 25.0%
!!! TGAAGATGCTTTTATTTAAG+AGG - chr8_3:10969947-10969966 None:intergenic 25.0%
!! GTTTTTAAATACATGGTTAC+TGG + chr8_3:10970092-10970111 Msa1276370:CDS 25.0%
! TTAATAGAGTATATGAGATC+TGG - chr8_3:10970131-10970150 None:intergenic 25.0%
! TTATAACAATACTTACTTGG+TGG - chr8_3:10970197-10970216 None:intergenic 25.0%
! GAGTTATAACAATACTTACT+TGG - chr8_3:10970200-10970219 None:intergenic 25.0%
! TTGATTGAGAGTAATAGTAA+GGG - chr8_3:10970682-10970701 None:intergenic 25.0%
! ATTGATTGAGAGTAATAGTA+AGG - chr8_3:10970683-10970702 None:intergenic 25.0%
!! TTGTACTTGATTGTTGATAA+AGG + chr8_3:10971020-10971039 Msa1276370:intron 25.0%
! ATAAATGATAATCAACCTGT+TGG - chr8_3:10971391-10971410 None:intergenic 25.0%
! CATTAACGCAATAGAAAAAA+CGG - chr8_3:10971526-10971545 None:intergenic 25.0%
!!! ATTTTGATCTTTAACTCTCT+TGG - chr8_3:10971623-10971642 None:intergenic 25.0%
! AAAGATCAAAATGTTATGTG+TGG + chr8_3:10971631-10971650 Msa1276370:three_prime_UTR 25.0%
! TGATCTTTATTGTGTTATGT+AGG + chr8_3:10971683-10971702 Msa1276370:three_prime_UTR 25.0%
! GATCTTTATTGTGTTATGTA+GGG + chr8_3:10971684-10971703 Msa1276370:three_prime_UTR 25.0%
! ATCTTTATTGTGTTATGTAG+GGG + chr8_3:10971685-10971704 Msa1276370:three_prime_UTR 25.0%
! TTTATTTAAGAGGGTTAAGA+GGG - chr8_3:10969937-10969956 None:intergenic 25.0%
!!! TTTTATTTAAGAGGGTTAAG+AGG - chr8_3:10969938-10969957 None:intergenic 25.0%
!!! GAAGATGCTTTTATTTAAGA+GGG - chr8_3:10969946-10969965 None:intergenic 25.0%
!!! TGAAGATGCTTTTATTTAAG+AGG - chr8_3:10969947-10969966 None:intergenic 25.0%
!! GTTTTTAAATACATGGTTAC+TGG + chr8_3:10970092-10970111 Msa1276370:CDS 25.0%
! TTAATAGAGTATATGAGATC+TGG - chr8_3:10970131-10970150 None:intergenic 25.0%
! TTATAACAATACTTACTTGG+TGG - chr8_3:10970197-10970216 None:intergenic 25.0%
! GAGTTATAACAATACTTACT+TGG - chr8_3:10970200-10970219 None:intergenic 25.0%
! TTGATTGAGAGTAATAGTAA+GGG - chr8_3:10970682-10970701 None:intergenic 25.0%
! ATTGATTGAGAGTAATAGTA+AGG - chr8_3:10970683-10970702 None:intergenic 25.0%
!! TTGTACTTGATTGTTGATAA+AGG + chr8_3:10971020-10971039 Msa1276370:intron 25.0%
! ATAAATGATAATCAACCTGT+TGG - chr8_3:10971391-10971410 None:intergenic 25.0%
! CATTAACGCAATAGAAAAAA+CGG - chr8_3:10971526-10971545 None:intergenic 25.0%
!!! ATTTTGATCTTTAACTCTCT+TGG - chr8_3:10971623-10971642 None:intergenic 25.0%
! AAAGATCAAAATGTTATGTG+TGG + chr8_3:10971631-10971650 Msa1276370:three_prime_UTR 25.0%
! TGATCTTTATTGTGTTATGT+AGG + chr8_3:10971683-10971702 Msa1276370:three_prime_UTR 25.0%
! GATCTTTATTGTGTTATGTA+GGG + chr8_3:10971684-10971703 Msa1276370:three_prime_UTR 25.0%
! ATCTTTATTGTGTTATGTAG+GGG + chr8_3:10971685-10971704 Msa1276370:three_prime_UTR 25.0%
TTATTTAAGAGGGTTAAGAG+GGG - chr8_3:10969936-10969955 None:intergenic 30.0%
!! AGCCCTAGTTTTTAAATACA+TGG + chr8_3:10970085-10970104 Msa1276370:CDS 30.0%
! TTTGTTGTCATAACTGTGAA+TGG + chr8_3:10970296-10970315 Msa1276370:intron 30.0%
!!! ATGATCAAGTGTTTTAGAAG+AGG - chr8_3:10970708-10970727 None:intergenic 30.0%
ATCTTATTCTCATGATGACA+AGG + chr8_3:10970781-10970800 Msa1276370:CDS 30.0%
GTATTTAGAGGTCTCTATAA+CGG - chr8_3:10970923-10970942 None:intergenic 30.0%
! TACTTGATTGTTGATAAAGG+AGG + chr8_3:10971023-10971042 Msa1276370:intron 30.0%
!!! GAACAACACTGTTTTGTTTT+AGG + chr8_3:10971226-10971245 Msa1276370:CDS 30.0%
AAACCACTTCATCATTTCTT+TGG + chr8_3:10971307-10971326 Msa1276370:CDS 30.0%
!! CAATAGAAAAAACGGACTAA+CGG - chr8_3:10971518-10971537 None:intergenic 30.0%
TATTTGATTAAGAGACTTGC+TGG + chr8_3:10971588-10971607 Msa1276370:three_prime_UTR 30.0%
! GGTGAATTTTCTTTAGCAAA+AGG + chr8_3:10971658-10971677 Msa1276370:three_prime_UTR 30.0%
TTATTTAAGAGGGTTAAGAG+GGG - chr8_3:10969936-10969955 None:intergenic 30.0%
!! AGCCCTAGTTTTTAAATACA+TGG + chr8_3:10970085-10970104 Msa1276370:CDS 30.0%
! TTTGTTGTCATAACTGTGAA+TGG + chr8_3:10970296-10970315 Msa1276370:intron 30.0%
!!! ATGATCAAGTGTTTTAGAAG+AGG - chr8_3:10970708-10970727 None:intergenic 30.0%
ATCTTATTCTCATGATGACA+AGG + chr8_3:10970781-10970800 Msa1276370:CDS 30.0%
GTATTTAGAGGTCTCTATAA+CGG - chr8_3:10970923-10970942 None:intergenic 30.0%
! TACTTGATTGTTGATAAAGG+AGG + chr8_3:10971023-10971042 Msa1276370:intron 30.0%
!!! GAACAACACTGTTTTGTTTT+AGG + chr8_3:10971226-10971245 Msa1276370:CDS 30.0%
AAACCACTTCATCATTTCTT+TGG + chr8_3:10971307-10971326 Msa1276370:CDS 30.0%
!! CAATAGAAAAAACGGACTAA+CGG - chr8_3:10971518-10971537 None:intergenic 30.0%
TATTTGATTAAGAGACTTGC+TGG + chr8_3:10971588-10971607 Msa1276370:three_prime_UTR 30.0%
! GGTGAATTTTCTTTAGCAAA+AGG + chr8_3:10971658-10971677 Msa1276370:three_prime_UTR 30.0%
ATTATTGTACTGTGCATGGA+TGG - chr8_3:10969907-10969926 None:intergenic 35.0%
TGAGTGCAAGTTCAAGAAAT+AGG + chr8_3:10970021-10970040 Msa1276370:CDS 35.0%
! CTCTTTTCACACCAAATCAA+TGG + chr8_3:10970045-10970064 Msa1276370:CDS 35.0%
! TCAAGTTCTTGCCATTGATT+TGG - chr8_3:10970059-10970078 None:intergenic 35.0%
TATATGAGATCTGGTGGAAT+AGG - chr8_3:10970122-10970141 None:intergenic 35.0%
ATAGAGTATATGAGATCTGG+TGG - chr8_3:10970128-10970147 None:intergenic 35.0%
!!! GGATGTTTTGAAATGGGATT+TGG + chr8_3:10970332-10970351 Msa1276370:CDS 35.0%
!! GCTGTGTTTGTGTTATTTGT+TGG - chr8_3:10970527-10970546 None:intergenic 35.0%
!! GTAATAGTAAGGGTTTTGGT+AGG - chr8_3:10970672-10970691 None:intergenic 35.0%
! GAGAGTAATAGTAAGGGTTT+TGG - chr8_3:10970676-10970695 None:intergenic 35.0%
! TACTACTCATCACCTCTTTT+TGG + chr8_3:10970751-10970770 Msa1276370:CDS 35.0%
AAGATGTTGAGTCCAAAAAG+AGG - chr8_3:10970766-10970785 None:intergenic 35.0%
! GAGACAACAATTTAGAACCA+TGG - chr8_3:10970842-10970861 None:intergenic 35.0%
GAGCTTGAGTAAGACTTAAA+GGG - chr8_3:10971163-10971182 None:intergenic 35.0%
TGAGCTTGAGTAAGACTTAA+AGG - chr8_3:10971164-10971183 None:intergenic 35.0%
GTCAACAAGAACAACAAACA+AGG + chr8_3:10971261-10971280 Msa1276370:CDS 35.0%
TCACCAAAGAAATGATGAAG+TGG - chr8_3:10971313-10971332 None:intergenic 35.0%
TTCATCATTTCTTTGGTGAG+TGG + chr8_3:10971314-10971333 Msa1276370:CDS 35.0%
ATCAAAATGTTATGTGTGGC+TGG + chr8_3:10971635-10971654 Msa1276370:three_prime_UTR 35.0%
TCAAAATGTTATGTGTGGCT+GGG + chr8_3:10971636-10971655 Msa1276370:three_prime_UTR 35.0%
!!! GGACCACATTAGGATTTTTT+TGG + chr8_3:10971706-10971725 Msa1276370:three_prime_UTR 35.0%
ATTATTGTACTGTGCATGGA+TGG - chr8_3:10969907-10969926 None:intergenic 35.0%
TGAGTGCAAGTTCAAGAAAT+AGG + chr8_3:10970021-10970040 Msa1276370:CDS 35.0%
! CTCTTTTCACACCAAATCAA+TGG + chr8_3:10970045-10970064 Msa1276370:CDS 35.0%
! TCAAGTTCTTGCCATTGATT+TGG - chr8_3:10970059-10970078 None:intergenic 35.0%
TATATGAGATCTGGTGGAAT+AGG - chr8_3:10970122-10970141 None:intergenic 35.0%
ATAGAGTATATGAGATCTGG+TGG - chr8_3:10970128-10970147 None:intergenic 35.0%
!!! GGATGTTTTGAAATGGGATT+TGG + chr8_3:10970332-10970351 Msa1276370:CDS 35.0%
!! GCTGTGTTTGTGTTATTTGT+TGG - chr8_3:10970527-10970546 None:intergenic 35.0%
!! GTAATAGTAAGGGTTTTGGT+AGG - chr8_3:10970672-10970691 None:intergenic 35.0%
! GAGAGTAATAGTAAGGGTTT+TGG - chr8_3:10970676-10970695 None:intergenic 35.0%
! TACTACTCATCACCTCTTTT+TGG + chr8_3:10970751-10970770 Msa1276370:CDS 35.0%
AAGATGTTGAGTCCAAAAAG+AGG - chr8_3:10970766-10970785 None:intergenic 35.0%
! GAGACAACAATTTAGAACCA+TGG - chr8_3:10970842-10970861 None:intergenic 35.0%
GAGCTTGAGTAAGACTTAAA+GGG - chr8_3:10971163-10971182 None:intergenic 35.0%
TGAGCTTGAGTAAGACTTAA+AGG - chr8_3:10971164-10971183 None:intergenic 35.0%
GTCAACAAGAACAACAAACA+AGG + chr8_3:10971261-10971280 Msa1276370:CDS 35.0%
TCACCAAAGAAATGATGAAG+TGG - chr8_3:10971313-10971332 None:intergenic 35.0%
TTCATCATTTCTTTGGTGAG+TGG + chr8_3:10971314-10971333 Msa1276370:CDS 35.0%
ATCAAAATGTTATGTGTGGC+TGG + chr8_3:10971635-10971654 Msa1276370:three_prime_UTR 35.0%
TCAAAATGTTATGTGTGGCT+GGG + chr8_3:10971636-10971655 Msa1276370:three_prime_UTR 35.0%
GGAGATTATTGTACTGTGCA+TGG - chr8_3:10969911-10969930 None:intergenic 40.0%
! CAATACTTACTTGGTGGATG+AGG - chr8_3:10970191-10970210 None:intergenic 40.0%
ATAACTGTGAATGGTGCAGT+TGG + chr8_3:10970305-10970324 Msa1276370:intron 40.0%
TAACTGTGAATGGTGCAGTT+GGG + chr8_3:10970306-10970325 Msa1276370:intron 40.0%
GAAGAACAGATGGCAAGAAA+TGG + chr8_3:10970387-10970406 Msa1276370:CDS 40.0%
CAAGAAATGGAGATGCTCAA+AGG + chr8_3:10970400-10970419 Msa1276370:CDS 40.0%
CTTTCACAGTACTTTGAGTC+TGG - chr8_3:10970434-10970453 None:intergenic 40.0%
GAAGAAACTACTAGTTCCAC+AGG - chr8_3:10970491-10970510 None:intergenic 40.0%
GTAGAGAAAGGGAAAGAAGA+AGG - chr8_3:10970605-10970624 None:intergenic 40.0%
CCATGGAAGAAGTAGAGAAA+GGG - chr8_3:10970616-10970635 None:intergenic 40.0%
CTCATGATGACAAGGACTAT+AGG + chr8_3:10970789-10970808 Msa1276370:CDS 40.0%
TGATAAAGGAGGCAAGTTCA+AGG + chr8_3:10971034-10971053 Msa1276370:intron 40.0%
GAGGCAAGTTCAAGGAATAA+GGG + chr8_3:10971042-10971061 Msa1276370:CDS 40.0%
CAAGTTCAAGGAATAAGGGA+TGG + chr8_3:10971046-10971065 Msa1276370:CDS 40.0%
ACTTTCTTTCCAGAAGCTAC+AGG + chr8_3:10971085-10971104 Msa1276370:CDS 40.0%
TAATCAACCTGTTGGAACTC+TGG - chr8_3:10971383-10971402 None:intergenic 40.0%
ATAACTTCACTCATCTGCCA+TGG - chr8_3:10971568-10971587 None:intergenic 40.0%
!! TTGATTAAGAGACTTGCTGG+TGG + chr8_3:10971591-10971610 Msa1276370:three_prime_UTR 40.0%
CAAAATGTTATGTGTGGCTG+GGG + chr8_3:10971637-10971656 Msa1276370:three_prime_UTR 40.0%
GGAGATTATTGTACTGTGCA+TGG - chr8_3:10969911-10969930 None:intergenic 40.0%
! CAATACTTACTTGGTGGATG+AGG - chr8_3:10970191-10970210 None:intergenic 40.0%
ATAACTGTGAATGGTGCAGT+TGG + chr8_3:10970305-10970324 Msa1276370:intron 40.0%
TAACTGTGAATGGTGCAGTT+GGG + chr8_3:10970306-10970325 Msa1276370:intron 40.0%
GAAGAACAGATGGCAAGAAA+TGG + chr8_3:10970387-10970406 Msa1276370:CDS 40.0%
CAAGAAATGGAGATGCTCAA+AGG + chr8_3:10970400-10970419 Msa1276370:CDS 40.0%
CTTTCACAGTACTTTGAGTC+TGG - chr8_3:10970434-10970453 None:intergenic 40.0%
GAAGAAACTACTAGTTCCAC+AGG - chr8_3:10970491-10970510 None:intergenic 40.0%
GTAGAGAAAGGGAAAGAAGA+AGG - chr8_3:10970605-10970624 None:intergenic 40.0%
CCATGGAAGAAGTAGAGAAA+GGG - chr8_3:10970616-10970635 None:intergenic 40.0%
CTCATGATGACAAGGACTAT+AGG + chr8_3:10970789-10970808 Msa1276370:CDS 40.0%
TGATAAAGGAGGCAAGTTCA+AGG + chr8_3:10971034-10971053 Msa1276370:intron 40.0%
GAGGCAAGTTCAAGGAATAA+GGG + chr8_3:10971042-10971061 Msa1276370:CDS 40.0%
CAAGTTCAAGGAATAAGGGA+TGG + chr8_3:10971046-10971065 Msa1276370:CDS 40.0%
ACTTTCTTTCCAGAAGCTAC+AGG + chr8_3:10971085-10971104 Msa1276370:CDS 40.0%
TAATCAACCTGTTGGAACTC+TGG - chr8_3:10971383-10971402 None:intergenic 40.0%
ATAACTTCACTCATCTGCCA+TGG - chr8_3:10971568-10971587 None:intergenic 40.0%
!! TTGATTAAGAGACTTGCTGG+TGG + chr8_3:10971591-10971610 Msa1276370:three_prime_UTR 40.0%
CAAAATGTTATGTGTGGCTG+GGG + chr8_3:10971637-10971656 Msa1276370:three_prime_UTR 40.0%
TTAAGAGGGTTAAGAGGGGT+TGG - chr8_3:10969932-10969951 None:intergenic 45.0%
!!! TGGGTGGGGATGTTTTGAAA+TGG + chr8_3:10970325-10970344 Msa1276370:CDS 45.0%
!!! GGGTGGGGATGTTTTGAAAT+GGG + chr8_3:10970326-10970345 Msa1276370:CDS 45.0%
TGTGAAAGACACATGCACAG+AGG + chr8_3:10970446-10970465 Msa1276370:CDS 45.0%
AAACCGTTCAAGAAAGCCTG+TGG + chr8_3:10970472-10970491 Msa1276370:CDS 45.0%
GTTCCACAGGCTTTCTTGAA+CGG - chr8_3:10970478-10970497 None:intergenic 45.0%
! GCTGTTGTTGTTGTTCAAGG+AGG - chr8_3:10970570-10970589 None:intergenic 45.0%
! GCTGCTGTTGTTGTTGTTCA+AGG - chr8_3:10970573-10970592 None:intergenic 45.0%
CCCTTTCTCTACTTCTTCCA+TGG + chr8_3:10970613-10970632 Msa1276370:CDS 45.0%
GCCATGGAAGAAGTAGAGAA+AGG - chr8_3:10970617-10970636 None:intergenic 45.0%
TGACTGATCATGACAAGCCA+TGG - chr8_3:10970633-10970652 None:intergenic 45.0%
GGAGGCAAGTTCAAGGAATA+AGG + chr8_3:10971041-10971060 Msa1276370:intron 45.0%
AGGAATAAGGGATGGTACTG+TGG + chr8_3:10971054-10971073 Msa1276370:CDS 45.0%
CTAGATGAACCTGTAGCTTC+TGG - chr8_3:10971097-10971116 None:intergenic 45.0%
CAGAAGCTACAGGTTCATCT+AGG + chr8_3:10971095-10971114 Msa1276370:CDS 45.0%
CACCAAAGAACACAGATTCC+TGG + chr8_3:10971338-10971357 Msa1276370:CDS 45.0%
AGCCAGGAATCTGTGTTCTT+TGG - chr8_3:10971343-10971362 None:intergenic 45.0%
GTTATGTAGGGGACCACATT+AGG + chr8_3:10971696-10971715 Msa1276370:three_prime_UTR 45.0%
TTAAGAGGGTTAAGAGGGGT+TGG - chr8_3:10969932-10969951 None:intergenic 45.0%
!!! TGGGTGGGGATGTTTTGAAA+TGG + chr8_3:10970325-10970344 Msa1276370:CDS 45.0%
!!! GGGTGGGGATGTTTTGAAAT+GGG + chr8_3:10970326-10970345 Msa1276370:CDS 45.0%
TGTGAAAGACACATGCACAG+AGG + chr8_3:10970446-10970465 Msa1276370:CDS 45.0%
AAACCGTTCAAGAAAGCCTG+TGG + chr8_3:10970472-10970491 Msa1276370:CDS 45.0%
GTTCCACAGGCTTTCTTGAA+CGG - chr8_3:10970478-10970497 None:intergenic 45.0%
! GCTGTTGTTGTTGTTCAAGG+AGG - chr8_3:10970570-10970589 None:intergenic 45.0%
! GCTGCTGTTGTTGTTGTTCA+AGG - chr8_3:10970573-10970592 None:intergenic 45.0%
CCCTTTCTCTACTTCTTCCA+TGG + chr8_3:10970613-10970632 Msa1276370:CDS 45.0%
GCCATGGAAGAAGTAGAGAA+AGG - chr8_3:10970617-10970636 None:intergenic 45.0%
TGACTGATCATGACAAGCCA+TGG - chr8_3:10970633-10970652 None:intergenic 45.0%
GGAGGCAAGTTCAAGGAATA+AGG + chr8_3:10971041-10971060 Msa1276370:intron 45.0%
AGGAATAAGGGATGGTACTG+TGG + chr8_3:10971054-10971073 Msa1276370:CDS 45.0%
CTAGATGAACCTGTAGCTTC+TGG - chr8_3:10971097-10971116 None:intergenic 45.0%
CAGAAGCTACAGGTTCATCT+AGG + chr8_3:10971095-10971114 Msa1276370:CDS 45.0%
CACCAAAGAACACAGATTCC+TGG + chr8_3:10971338-10971357 Msa1276370:CDS 45.0%
AGCCAGGAATCTGTGTTCTT+TGG - chr8_3:10971343-10971362 None:intergenic 45.0%
GTTATGTAGGGGACCACATT+AGG + chr8_3:10971696-10971715 Msa1276370:three_prime_UTR 45.0%
!!! TTTTTAATATTTTATGAAAA+AGG + chr8_3:10970220-10970239 Msa1276370:intron 5.0%
!! AATAATATTAAATAACTTTA+TGG - chr8_3:10971460-10971479 None:intergenic 5.0%
!!! TTTTTAATATTTTATGAAAA+AGG + chr8_3:10970220-10970239 Msa1276370:intron 5.0%
!! AATAATATTAAATAACTTTA+TGG - chr8_3:10971460-10971479 None:intergenic 5.0%
TGTGAATGGTGCAGTTGGGT+GGG + chr8_3:10970310-10970329 Msa1276370:intron 50.0%
AGAAAAGTAGACCCAGAGCC+AGG + chr8_3:10970356-10970375 Msa1276370:CDS 50.0%
GAAAAGTAGACCCAGAGCCA+GGG + chr8_3:10970357-10970376 Msa1276370:CDS 50.0%
GTTGGAAGCAAGATCTAGCC+AGG - chr8_3:10971359-10971378 None:intergenic 50.0%
TCCAACTCCAGAGTTCCAAC+AGG + chr8_3:10971373-10971392 Msa1276370:CDS 50.0%
!! ACCTGTTGGAACTCTGGAGT+TGG - chr8_3:10971377-10971396 None:intergenic 50.0%
TGTGAATGGTGCAGTTGGGT+GGG + chr8_3:10970310-10970329 Msa1276370:intron 50.0%
AGAAAAGTAGACCCAGAGCC+AGG + chr8_3:10970356-10970375 Msa1276370:CDS 50.0%
GAAAAGTAGACCCAGAGCCA+GGG + chr8_3:10970357-10970376 Msa1276370:CDS 50.0%
GTTGGAAGCAAGATCTAGCC+AGG - chr8_3:10971359-10971378 None:intergenic 50.0%
TCCAACTCCAGAGTTCCAAC+AGG + chr8_3:10971373-10971392 Msa1276370:CDS 50.0%
!! ACCTGTTGGAACTCTGGAGT+TGG - chr8_3:10971377-10971396 None:intergenic 50.0%
CTGTGAATGGTGCAGTTGGG+TGG + chr8_3:10970309-10970328 Msa1276370:intron 55.0%
GTGAATGGTGCAGTTGGGTG+GGG + chr8_3:10970311-10970330 Msa1276370:intron 55.0%
TCTGTTCTTCTGCACCTCCC+TGG - chr8_3:10970377-10970396 None:intergenic 55.0%
! GGGAGGTGCAGAAGAACAGA+TGG + chr8_3:10970377-10970396 Msa1276370:CDS 55.0%
!! CGGACACAGAAGTACTCGTC+TGG - chr8_3:10971498-10971517 None:intergenic 55.0%
CTGTGAATGGTGCAGTTGGG+TGG + chr8_3:10970309-10970328 Msa1276370:intron 55.0%
GTGAATGGTGCAGTTGGGTG+GGG + chr8_3:10970311-10970330 Msa1276370:intron 55.0%
TCTGTTCTTCTGCACCTCCC+TGG - chr8_3:10970377-10970396 None:intergenic 55.0%
! GGGAGGTGCAGAAGAACAGA+TGG + chr8_3:10970377-10970396 Msa1276370:CDS 55.0%
!! CGGACACAGAAGTACTCGTC+TGG - chr8_3:10971498-10971517 None:intergenic 55.0%
AAGTAGACCCAGAGCCAGGG+AGG + chr8_3:10970360-10970379 Msa1276370:CDS 60.0%
! TTCTGCACCTCCCTGGCTCT+GGG - chr8_3:10970370-10970389 None:intergenic 60.0%
AAGTAGACCCAGAGCCAGGG+AGG + chr8_3:10970360-10970379 Msa1276370:CDS 60.0%
! TTCTGCACCTCCCTGGCTCT+GGG - chr8_3:10970370-10970389 None:intergenic 60.0%
CTTCTGCACCTCCCTGGCTC+TGG - chr8_3:10970371-10970390 None:intergenic 65.0%
CTTCTGCACCTCCCTGGCTC+TGG - chr8_3:10970371-10970390 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 10969868 10971725 10969868 ID=Msa1276370;Name=Msa1276370
chr8_3 mRNA 10969868 10971725 10969868 ID=Msa1276370-mRNA-1;Parent=Msa1276370;Name=Msa1276370-mRNA-1;_AED=0.05;_eAED=0.05;_QI=149|0.75|0.4|1|0.5|0.4|5|144|339
chr8_3 exon 10969868 10970200 10969868 ID=Msa1276370-mRNA-1:exon:4477;Parent=Msa1276370-mRNA-1
chr8_3 exon 10970324 10970810 10970324 ID=Msa1276370-mRNA-1:exon:4478;Parent=Msa1276370-mRNA-1
chr8_3 exon 10971042 10971167 10971042 ID=Msa1276370-mRNA-1:exon:4479;Parent=Msa1276370-mRNA-1
chr8_3 exon 10971180 10971394 10971180 ID=Msa1276370-mRNA-1:exon:4480;Parent=Msa1276370-mRNA-1
chr8_3 exon 10971574 10971725 10971574 ID=Msa1276370-mRNA-1:exon:4481;Parent=Msa1276370-mRNA-1
chr8_3 five_prime_UTR 10969868 10970016 10969868 ID=Msa1276370-mRNA-1:five_prime_utr;Parent=Msa1276370-mRNA-1
chr8_3 CDS 10970017 10970200 10970017 ID=Msa1276370-mRNA-1:cds;Parent=Msa1276370-mRNA-1
chr8_3 CDS 10970324 10970810 10970324 ID=Msa1276370-mRNA-1:cds;Parent=Msa1276370-mRNA-1
chr8_3 CDS 10971042 10971167 10971042 ID=Msa1276370-mRNA-1:cds;Parent=Msa1276370-mRNA-1
chr8_3 CDS 10971180 10971394 10971180 ID=Msa1276370-mRNA-1:cds;Parent=Msa1276370-mRNA-1
chr8_3 CDS 10971574 10971581 10971574 ID=Msa1276370-mRNA-1:cds;Parent=Msa1276370-mRNA-1
chr8_3 three_prime_UTR 10971582 10971725 10971582 ID=Msa1276370-mRNA-1:three_prime_utr;Parent=Msa1276370-mRNA-1
Gene Sequence

>Msa1276370

ATGATGAGTGCAAGTTCAAGAAATAGGTCTCTTTTCACACCAAATCAATGGCAAGAACTTGAACAACAAGCCCTAGTTTTTAAATACATGGTTACTGGAACACCTATTCCACCAGATCTCATATACTCTATTAAGAGAAGTTTAGACACTTCAATATCTTCAAGAATCTTTCCTCATCCACCAATTGGGTGGGGATGTTTTGAAATGGGATTTGGCAGAAAAGTAGACCCAGAGCCAGGGAGGTGCAGAAGAACAGATGGCAAGAAATGGAGATGCTCAAAGGAAGCATATCCAGACTCAAAGTACTGTGAAAGACACATGCACAGAGGTAGAAACCGTTCAAGAAAGCCTGTGGAACTAGTAGTTTCTTCTTCAACAGCAACACCAACAAATAACACAAACACAGCATCTTCTTACAACAGAAACACCTCCTTGAACAACAACAACAGCAGCATAAACTCACCTTCTTCTTTCCCTTTCTCTACTTCTTCCATGGCTTGTCATGATCAGTCACAATCTTTTTCACAATCCTACCAAAACCCTTACTATTACTCTCAATCAATTACCTCTTCTAAAACACTTGATCATTCTGATTTTCAAACTCAAGATGCTACTACTCATCACCTCTTTTTGGACTCAACATCTTATTCTCATGATGACAAGGACTATAGGAGGCAAGTTCAAGGAATAAGGGATGGTACTGTGGATGAGAGAACTTTCTTTCCAGAAGCTACAGGTTCATCTAGGAGCTGCTATCATGATTCATATCAACAACAACTATCAATGAATCCCTTTAACTCACAGTTTCAAAATATCAATGATGATAATTCAAGACAACAACAAGAACAACACTGTTTTGTTTTAGGCACTGACATCAAGTCAACAAGAACAACAAACAAGGACAAAGAAAGTGAGACAACTCAGAAACCACTTCATCATTTCTTTGGTGAGTGGACACCAAAGAACACAGATTCCTGGCTAGATCTTGCTTCCAACTCCAGAGTTCCAACAGATGAGTGA
Protein sequence

>Msa1276370

MMSASSRNRSLFTPNQWQELEQQALVFKYMVTGTPIPPDLIYSIKRSLDTSISSRIFPHPPIGWGCFEMGFGRKVDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVELVVSSSTATPTNNTNTASSYNRNTSLNNNNSSINSPSSFPFSTSSMACHDQSQSFSQSYQNPYYYSQSITSSKTLDHSDFQTQDATTHHLFLDSTSYSHDDKDYRRQVQGIRDGTVDERTFFPEATGSSRSCYHDSYQQQLSMNPFNSQFQNINDDNSRQQQEQHCFVLGTDIKSTRTTNKDKESETTQKPLHHFFGEWTPKNTDSWLDLASNSRVPTDE*