AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1234140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234140 MtrunA17_Chr8g0343881 95.690 348 8 4 1 341 1 348 0.0 676
Msa1234140 MtrunA17_Chr4g0070591 43.697 357 155 15 1 331 1 337 1.09e-74 234
Msa1234140 MtrunA17_Chr2g0300511 43.491 338 163 11 7 331 3 325 3.23e-69 220
Msa1234140 MtrunA17_Chr4g0021261 39.571 326 144 12 10 331 24 300 1.13e-54 182
Msa1234140 MtrunA17_Chr1g0152191 52.846 123 54 1 1 119 134 256 1.29e-40 150
Msa1234140 MtrunA17_Chr5g0409471 58.333 108 33 2 12 119 17 112 2.01e-39 142
Msa1234140 MtrunA17_Chr7g0265931 54.918 122 46 4 12 131 79 193 6.43e-36 134
Msa1234140 MtrunA17_Chr3g0127641 47.706 109 39 2 12 120 113 203 2.15e-28 115
Msa1234140 MtrunA17_Chr8g0351521 38.000 150 77 2 8 157 51 184 2.67e-21 94.4
Msa1234140 MtrunA17_Chr8g0351521 66.667 39 13 0 75 113 302 340 1.28e-12 68.6
Msa1234140 MtrunA17_Chr8g0351291 64.103 39 14 0 75 113 51 89 1.20e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234140 AT3G13960.1 41.033 368 163 10 3 319 8 372 5.80e-71 226
Msa1234140 AT2G06200.2 57.364 129 40 3 2 117 36 162 3.81e-43 150
Msa1234140 AT2G06200.1 59.836 122 34 3 9 117 4 123 6.11e-43 149
Msa1234140 AT4G37740.1 49.342 152 55 4 12 144 165 313 4.45e-40 148
Msa1234140 AT2G22840.1 57.018 114 35 4 12 119 134 239 3.49e-35 134
Msa1234140 AT2G36400.1 51.181 127 47 4 10 127 75 195 8.48e-35 131
Msa1234140 AT3G52910.1 40.909 198 95 6 11 194 82 271 1.41e-32 125
Msa1234140 AT2G06200.3 88.235 51 4 1 69 117 1 51 2.83e-25 100
Msa1234140 AT5G53660.1 47.222 108 40 1 12 119 60 150 8.83e-25 103
Msa1234140 AT4G24150.3 41.739 115 61 2 10 119 110 223 2.11e-24 103
Msa1234140 AT4G24150.2 41.739 115 61 2 10 119 148 261 3.57e-24 103
Msa1234140 AT4G24150.1 37.143 140 57 3 10 119 148 286 4.56e-22 97.1
Msa1234140 AT2G45480.2 46.535 101 54 0 16 116 29 129 6.30e-16 77.8
Msa1234140 AT2G45480.3 37.419 155 89 1 16 162 29 183 1.39e-15 77.8
Msa1234140 AT2G45480.3 47.500 80 31 3 40 119 281 349 2.12e-14 74.3
Msa1234140 AT2G45480.1 37.419 155 89 1 16 162 27 181 1.55e-15 77.8
Msa1234140 AT2G45480.1 47.500 80 31 3 40 119 279 347 2.13e-14 74.3

Find 72 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCTTGAGTAAGACTTAA+AGG 0.238026 8_2:+11362578 None:intergenic
GGAGGCAAGTTCAAGGAATA+AGG 0.269692 8_2:-11362698 Msa1234140:CDS
CTTCTGCACCTCCCTGGCTC+TGG 0.278943 8_2:+11363367 None:intergenic
AAACCACTTCATCATTTCTT+TGG 0.279787 8_2:-11362435 Msa1234140:CDS
AGCCAGGAATCTGTGTTCTT+TGG 0.341985 8_2:+11362402 None:intergenic
CTTTCACAGTACTTTGAGTC+TGG 0.352106 8_2:+11363304 None:intergenic
CTAGATGAACCTGTAGCTTC+TGG 0.361439 8_2:+11362645 None:intergenic
CCCTTTCTCTACTTCTTCCA+TGG 0.361986 8_2:-11363125 Msa1234140:CDS
GAGTAGTAGCATCTTGAGTT+TGG 0.362099 8_2:+11363002 None:intergenic
TATTTGATTAAGAGACTTGC+TGG 0.365421 8_2:-11362154 Msa1234140:three_prime_UTR
GCTGTGTTTGTGTTATTTGT+TGG 0.366262 8_2:+11363214 None:intergenic
TTCTGCACCTCCCTGGCTCT+GGG 0.368474 8_2:+11363368 None:intergenic
GAGAGTAATAGTAAGGGTTT+TGG 0.368535 8_2:+11363065 None:intergenic
GCTGCTGTTGTTGTTGTTCA+AGG 0.381104 8_2:+11363168 None:intergenic
TTAAGAGGGTTAAGAGGGGT+TGG 0.387463 8_2:+11363806 None:intergenic
GTTATGTAGGGGACCACATT+AGG 0.395401 8_2:-11362046 Msa1234140:three_prime_UTR
TGATCTTTATTGTGTTATGT+AGG 0.395473 8_2:-11362059 Msa1234140:three_prime_UTR
TATATGAGATCTGGTGGAAT+AGG 0.406485 8_2:+11363616 None:intergenic
AGAAAAGTAGACCCAGAGCC+AGG 0.407864 8_2:-11363379 Msa1234140:CDS
TCAAGTTCTTGCCATTGATT+TGG 0.410002 8_2:+11363679 None:intergenic
ATTGATTGAGAGTAATAGTA+AGG 0.410096 8_2:+11363058 None:intergenic
GAGCTTGAGTAAGACTTAAA+GGG 0.410732 8_2:+11362579 None:intergenic
GAAGAACAGATGGCAAGAAA+TGG 0.429768 8_2:-11363348 Msa1234140:CDS
ATCAAAATGTTATGTGTGGC+TGG 0.435666 8_2:-11362107 Msa1234140:three_prime_UTR
CAGAAGCTACAGGTTCATCT+AGG 0.438969 8_2:-11362644 Msa1234140:CDS
GATCTTTATTGTGTTATGTA+GGG 0.443907 8_2:-11362058 Msa1234140:three_prime_UTR
TGAGTGCAAGTTCAAGAAAT+AGG 0.444135 8_2:-11363714 Msa1234140:CDS
ACTTTCTTTCCAGAAGCTAC+AGG 0.451806 8_2:-11362654 Msa1234140:CDS
TCTGTTCTTCTGCACCTCCC+TGG 0.454328 8_2:+11363361 None:intergenic
TTTATTTAAGAGGGTTAAGA+GGG 0.462557 8_2:+11363801 None:intergenic
TTAATAGAGTATATGAGATC+TGG 0.465803 8_2:+11363607 None:intergenic
TAATCAACCTGTTGGAACTC+TGG 0.470845 8_2:+11362362 None:intergenic
CACCAAAGAACACAGATTCC+TGG 0.474579 8_2:-11362404 Msa1234140:CDS
CTCATGATGACAAGGACTAT+AGG 0.477649 8_2:-11362949 Msa1234140:intron
CAATACTTACTTGGTGGATG+AGG 0.492982 8_2:+11363547 None:intergenic
GCCATGGAAGAAGTAGAGAA+AGG 0.495080 8_2:+11363124 None:intergenic
ACCTGTTGGAACTCTGGAGT+TGG 0.501428 8_2:+11362368 None:intergenic
TCCAACTCCAGAGTTCCAAC+AGG 0.510080 8_2:-11362369 Msa1234140:intron
GTAGAGAAAGGGAAAGAAGA+AGG 0.511856 8_2:+11363136 None:intergenic
CCATGGAAGAAGTAGAGAAA+GGG 0.516371 8_2:+11363125 None:intergenic
TGATAAAGGAGGCAAGTTCA+AGG 0.519079 8_2:-11362705 Msa1234140:intron
GAGGCAAGTTCAAGGAATAA+GGG 0.523074 8_2:-11362697 Msa1234140:CDS
GTTCCACAGGCTTTCTTGAA+CGG 0.529120 8_2:+11363260 None:intergenic
ATCTTTATTGTGTTATGTAG+GGG 0.533482 8_2:-11362057 Msa1234140:three_prime_UTR
TGTGAATGGTGCAGTTGGGT+GGG 0.535441 8_2:-11363425 Msa1234140:intron
TTCATCATTTCTTTGGTGAG+TGG 0.536164 8_2:-11362428 Msa1234140:CDS
GTTGGAAGCAAGATCTAGCC+AGG 0.536185 8_2:+11362386 None:intergenic
GTGAATGGTGCAGTTGGGTG+GGG 0.550789 8_2:-11363424 Msa1234140:intron
GAAAAGTAGACCCAGAGCCA+GGG 0.561252 8_2:-11363378 Msa1234140:CDS
ATTATTGTACTGTGCATGGA+TGG 0.567364 8_2:+11363831 None:intergenic
TCACCAAAGAAATGATGAAG+TGG 0.571081 8_2:+11362432 None:intergenic
TCAAAATGTTATGTGTGGCT+GGG 0.576383 8_2:-11362106 Msa1234140:three_prime_UTR
CTGTGAATGGTGCAGTTGGG+TGG 0.577092 8_2:-11363426 Msa1234140:intron
GGAGATTATTGTACTGTGCA+TGG 0.577269 8_2:+11363827 None:intergenic
ATAGAGTATATGAGATCTGG+TGG 0.579210 8_2:+11363610 None:intergenic
GAAGAAACTACTAGTTCCAC+AGG 0.590035 8_2:+11363247 None:intergenic
CAAGAAATGGAGATGCTCAA+AGG 0.592887 8_2:-11363335 Msa1234140:CDS
TTGATTGAGAGTAATAGTAA+GGG 0.594306 8_2:+11363059 None:intergenic
GGGAGGTGCAGAAGAACAGA+TGG 0.600412 8_2:-11363358 Msa1234140:CDS
ATCTTATTCTCATGATGACA+AGG 0.605341 8_2:-11362957 Msa1234140:CDS
TGACTGATCATGACAAGCCA+TGG 0.611257 8_2:+11363108 None:intergenic
CAAAATGTTATGTGTGGCTG+GGG 0.614659 8_2:-11362105 Msa1234140:three_prime_UTR
AGGAATAAGGGATGGTACTG+TGG 0.625865 8_2:-11362685 Msa1234140:CDS
TTGATTAAGAGACTTGCTGG+TGG 0.637573 8_2:-11362151 Msa1234140:three_prime_UTR
CAAGTTCAAGGAATAAGGGA+TGG 0.642231 8_2:-11362693 Msa1234140:CDS
GCTGTTGTTGTTGTTCAAGG+AGG 0.650068 8_2:+11363171 None:intergenic
AAACCGTTCAAGAAAGCCTG+TGG 0.653157 8_2:-11363263 Msa1234140:CDS
AAGTAGACCCAGAGCCAGGG+AGG 0.659120 8_2:-11363375 Msa1234140:CDS
GTCAACAAGAACAACAAACA+AGG 0.662924 8_2:-11362481 Msa1234140:CDS
TTATTTAAGAGGGTTAAGAG+GGG 0.668991 8_2:+11363802 None:intergenic
AAAGATCAAAATGTTATGTG+TGG 0.684063 8_2:-11362111 Msa1234140:three_prime_UTR
TGTGAAAGACACATGCACAG+AGG 0.772839 8_2:-11363289 Msa1234140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATATTTATATCAAATG+CGG - chr8_2:11363045-11363064 Msa1234140:CDS 10.0%
!!! TTTTATATTTATATCAAATG+CGG - chr8_2:11363045-11363064 Msa1234140:CDS 10.0%
!!! TGATTTTAAATTGTATTTAG+AGG + chr8_2:11363103-11363122 None:intergenic 15.0%
!! TTTATATACTTTATTATCCA+TGG - chr8_2:11363712-11363731 Msa1234140:CDS 15.0%
!!! TGATTTTAAATTGTATTTAG+AGG + chr8_2:11363103-11363122 None:intergenic 15.0%
!! TTTATATACTTTATTATCCA+TGG - chr8_2:11363712-11363731 Msa1234140:CDS 15.0%
!!! AAAAATAAGGATTTTCTTGA+TGG + chr8_2:11362156-11362175 None:intergenic 20.0%
!!! TTTTTTGAGACACAAAAATA+AGG + chr8_2:11362169-11362188 None:intergenic 20.0%
!! AACCATGTATTTAAAAACTA+GGG + chr8_2:11362261-11362280 None:intergenic 20.0%
!! TAACCATGTATTTAAAAACT+AGG + chr8_2:11362262-11362281 None:intergenic 20.0%
!! ACCAATAAATATATATACCA+TGG - chr8_2:11362990-11363009 Msa1234140:CDS 20.0%
!! ACCATGGTATATATATTTAT+TGG + chr8_2:11362994-11363013 None:intergenic 20.0%
!! GAACAAAAAAACAAAAACAT+AGG + chr8_2:11363582-11363601 None:intergenic 20.0%
!!! AAAAATAAGGATTTTCTTGA+TGG + chr8_2:11362156-11362175 None:intergenic 20.0%
!!! TTTTTTGAGACACAAAAATA+AGG + chr8_2:11362169-11362188 None:intergenic 20.0%
!! AACCATGTATTTAAAAACTA+GGG + chr8_2:11362261-11362280 None:intergenic 20.0%
!! TAACCATGTATTTAAAAACT+AGG + chr8_2:11362262-11362281 None:intergenic 20.0%
!! ACCAATAAATATATATACCA+TGG - chr8_2:11362990-11363009 Msa1234140:CDS 20.0%
!! ACCATGGTATATATATTTAT+TGG + chr8_2:11362994-11363013 None:intergenic 20.0%
!! GAACAAAAAAACAAAAACAT+AGG + chr8_2:11363582-11363601 None:intergenic 20.0%
! TTTATTTAAGAGGGTTAAGA+GGG + chr8_2:11362108-11362127 None:intergenic 25.0%
!!! TTTTATTTAAGAGGGTTAAG+AGG + chr8_2:11362109-11362128 None:intergenic 25.0%
!!! GAAGATGCTTTTATTTAAGA+GGG + chr8_2:11362117-11362136 None:intergenic 25.0%
!!! TGAAGATGCTTTTATTTAAG+AGG + chr8_2:11362118-11362137 None:intergenic 25.0%
!! GTTTTTAAATACATGGTTAC+TGG - chr8_2:11362263-11362282 Msa1234140:intron 25.0%
! TTAATAGAGTATATGAGATC+TGG + chr8_2:11362302-11362321 None:intergenic 25.0%
! TTATAACAATACTTACTTGG+TGG + chr8_2:11362368-11362387 None:intergenic 25.0%
! GAGTTATAACAATACTTACT+TGG + chr8_2:11362371-11362390 None:intergenic 25.0%
! TTGATTGAGAGTAATAGTAA+GGG + chr8_2:11362850-11362869 None:intergenic 25.0%
! ATTGATTGAGAGTAATAGTA+AGG + chr8_2:11362851-11362870 None:intergenic 25.0%
!! TTGTACTTGATTGTTGATAA+AGG - chr8_2:11363187-11363206 Msa1234140:CDS 25.0%
! ATAAATGATAATCAACCTGT+TGG + chr8_2:11363555-11363574 None:intergenic 25.0%
! CATTAACGCAATAGAAAAAA+CGG + chr8_2:11363690-11363709 None:intergenic 25.0%
!!! ATTTTGATCTTTAACTCTCT+TGG + chr8_2:11363787-11363806 None:intergenic 25.0%
! AAAGATCAAAATGTTATGTG+TGG - chr8_2:11363795-11363814 Msa1234140:five_prime_UTR 25.0%
! TGATCTTTATTGTGTTATGT+AGG - chr8_2:11363847-11363866 Msa1234140:five_prime_UTR 25.0%
! GATCTTTATTGTGTTATGTA+GGG - chr8_2:11363848-11363867 Msa1234140:five_prime_UTR 25.0%
! ATCTTTATTGTGTTATGTAG+GGG - chr8_2:11363849-11363868 Msa1234140:five_prime_UTR 25.0%
! TTTATTTAAGAGGGTTAAGA+GGG + chr8_2:11362108-11362127 None:intergenic 25.0%
!!! TTTTATTTAAGAGGGTTAAG+AGG + chr8_2:11362109-11362128 None:intergenic 25.0%
!!! GAAGATGCTTTTATTTAAGA+GGG + chr8_2:11362117-11362136 None:intergenic 25.0%
!!! TGAAGATGCTTTTATTTAAG+AGG + chr8_2:11362118-11362137 None:intergenic 25.0%
!! GTTTTTAAATACATGGTTAC+TGG - chr8_2:11362263-11362282 Msa1234140:intron 25.0%
! TTAATAGAGTATATGAGATC+TGG + chr8_2:11362302-11362321 None:intergenic 25.0%
! TTATAACAATACTTACTTGG+TGG + chr8_2:11362368-11362387 None:intergenic 25.0%
! GAGTTATAACAATACTTACT+TGG + chr8_2:11362371-11362390 None:intergenic 25.0%
! TTGATTGAGAGTAATAGTAA+GGG + chr8_2:11362850-11362869 None:intergenic 25.0%
! ATTGATTGAGAGTAATAGTA+AGG + chr8_2:11362851-11362870 None:intergenic 25.0%
!! TTGTACTTGATTGTTGATAA+AGG - chr8_2:11363187-11363206 Msa1234140:CDS 25.0%
! ATAAATGATAATCAACCTGT+TGG + chr8_2:11363555-11363574 None:intergenic 25.0%
! CATTAACGCAATAGAAAAAA+CGG + chr8_2:11363690-11363709 None:intergenic 25.0%
!!! ATTTTGATCTTTAACTCTCT+TGG + chr8_2:11363787-11363806 None:intergenic 25.0%
! AAAGATCAAAATGTTATGTG+TGG - chr8_2:11363795-11363814 Msa1234140:five_prime_UTR 25.0%
! TGATCTTTATTGTGTTATGT+AGG - chr8_2:11363847-11363866 Msa1234140:five_prime_UTR 25.0%
! GATCTTTATTGTGTTATGTA+GGG - chr8_2:11363848-11363867 Msa1234140:five_prime_UTR 25.0%
! ATCTTTATTGTGTTATGTAG+GGG - chr8_2:11363849-11363868 Msa1234140:five_prime_UTR 25.0%
TTATTTAAGAGGGTTAAGAG+GGG + chr8_2:11362107-11362126 None:intergenic 30.0%
!! AGCCCTAGTTTTTAAATACA+TGG - chr8_2:11362256-11362275 Msa1234140:intron 30.0%
! TTTGTTGTCATAACTGTGAA+TGG - chr8_2:11362467-11362486 Msa1234140:CDS 30.0%
!!! ATGATCAAGTGTTTTAGAAG+AGG + chr8_2:11362876-11362895 None:intergenic 30.0%
ATCTTATTCTCATGATGACA+AGG - chr8_2:11362949-11362968 Msa1234140:intron 30.0%
GTATTTAGAGGTCTCTATAA+CGG + chr8_2:11363091-11363110 None:intergenic 30.0%
! TACTTGATTGTTGATAAAGG+AGG - chr8_2:11363190-11363209 Msa1234140:CDS 30.0%
!!! GAACAACACTGTTTTGTTTT+AGG - chr8_2:11363390-11363409 Msa1234140:CDS 30.0%
AAACCACTTCATCATTTCTT+TGG - chr8_2:11363471-11363490 Msa1234140:intron 30.0%
TATTTGATTAAGAGACTTGC+TGG - chr8_2:11363752-11363771 Msa1234140:five_prime_UTR 30.0%
! GGTGAATTTTCTTTAGCAAA+AGG - chr8_2:11363822-11363841 Msa1234140:five_prime_UTR 30.0%
TTATTTAAGAGGGTTAAGAG+GGG + chr8_2:11362107-11362126 None:intergenic 30.0%
!! AGCCCTAGTTTTTAAATACA+TGG - chr8_2:11362256-11362275 Msa1234140:intron 30.0%
! TTTGTTGTCATAACTGTGAA+TGG - chr8_2:11362467-11362486 Msa1234140:CDS 30.0%
!!! ATGATCAAGTGTTTTAGAAG+AGG + chr8_2:11362876-11362895 None:intergenic 30.0%
ATCTTATTCTCATGATGACA+AGG - chr8_2:11362949-11362968 Msa1234140:intron 30.0%
GTATTTAGAGGTCTCTATAA+CGG + chr8_2:11363091-11363110 None:intergenic 30.0%
! TACTTGATTGTTGATAAAGG+AGG - chr8_2:11363190-11363209 Msa1234140:CDS 30.0%
!!! GAACAACACTGTTTTGTTTT+AGG - chr8_2:11363390-11363409 Msa1234140:CDS 30.0%
AAACCACTTCATCATTTCTT+TGG - chr8_2:11363471-11363490 Msa1234140:intron 30.0%
TATTTGATTAAGAGACTTGC+TGG - chr8_2:11363752-11363771 Msa1234140:five_prime_UTR 30.0%
! GGTGAATTTTCTTTAGCAAA+AGG - chr8_2:11363822-11363841 Msa1234140:five_prime_UTR 30.0%
ATTATTGTACTGTGCATGGA+TGG + chr8_2:11362078-11362097 None:intergenic 35.0%
TGAGTGCAAGTTCAAGAAAT+AGG - chr8_2:11362192-11362211 Msa1234140:intron 35.0%
! CTCTTTTCACACCAAATCAA+TGG - chr8_2:11362216-11362235 Msa1234140:intron 35.0%
! TCAAGTTCTTGCCATTGATT+TGG + chr8_2:11362230-11362249 None:intergenic 35.0%
TATATGAGATCTGGTGGAAT+AGG + chr8_2:11362293-11362312 None:intergenic 35.0%
ATAGAGTATATGAGATCTGG+TGG + chr8_2:11362299-11362318 None:intergenic 35.0%
!!! GGATGTTTTGAAATGGGATT+TGG - chr8_2:11362503-11362522 Msa1234140:CDS 35.0%
!! GCTGTGTTTGTGTTATTTGT+TGG + chr8_2:11362695-11362714 None:intergenic 35.0%
!! GTAATAGTAAGGGTTTTGGT+AGG + chr8_2:11362840-11362859 None:intergenic 35.0%
! GAGAGTAATAGTAAGGGTTT+TGG + chr8_2:11362844-11362863 None:intergenic 35.0%
! TACTACTCATCACCTCTTTT+TGG - chr8_2:11362919-11362938 Msa1234140:intron 35.0%
AAGATGTTGAGTCCAAAAAG+AGG + chr8_2:11362934-11362953 None:intergenic 35.0%
! GAGACAACAATTTAGAACCA+TGG + chr8_2:11363010-11363029 None:intergenic 35.0%
GAGCTTGAGTAAGACTTAAA+GGG + chr8_2:11363330-11363349 None:intergenic 35.0%
TGAGCTTGAGTAAGACTTAA+AGG + chr8_2:11363331-11363350 None:intergenic 35.0%
GTCAACAAGAACAACAAACA+AGG - chr8_2:11363425-11363444 Msa1234140:intron 35.0%
TCACCAAAGAAATGATGAAG+TGG + chr8_2:11363477-11363496 None:intergenic 35.0%
TTCATCATTTCTTTGGTGAG+TGG - chr8_2:11363478-11363497 Msa1234140:intron 35.0%
CAATAGAAAAAACGGACAGA+CGG + chr8_2:11363682-11363701 None:intergenic 35.0%
ATCAAAATGTTATGTGTGGC+TGG - chr8_2:11363799-11363818 Msa1234140:five_prime_UTR 35.0%
TCAAAATGTTATGTGTGGCT+GGG - chr8_2:11363800-11363819 Msa1234140:five_prime_UTR 35.0%
!!! GGACCACATTAGGATTTTTT+TGG - chr8_2:11363870-11363889 Msa1234140:five_prime_UTR 35.0%
ATTATTGTACTGTGCATGGA+TGG + chr8_2:11362078-11362097 None:intergenic 35.0%
TGAGTGCAAGTTCAAGAAAT+AGG - chr8_2:11362192-11362211 Msa1234140:intron 35.0%
! CTCTTTTCACACCAAATCAA+TGG - chr8_2:11362216-11362235 Msa1234140:intron 35.0%
! TCAAGTTCTTGCCATTGATT+TGG + chr8_2:11362230-11362249 None:intergenic 35.0%
TATATGAGATCTGGTGGAAT+AGG + chr8_2:11362293-11362312 None:intergenic 35.0%
ATAGAGTATATGAGATCTGG+TGG + chr8_2:11362299-11362318 None:intergenic 35.0%
!!! GGATGTTTTGAAATGGGATT+TGG - chr8_2:11362503-11362522 Msa1234140:CDS 35.0%
!! GCTGTGTTTGTGTTATTTGT+TGG + chr8_2:11362695-11362714 None:intergenic 35.0%
!! GTAATAGTAAGGGTTTTGGT+AGG + chr8_2:11362840-11362859 None:intergenic 35.0%
! GAGAGTAATAGTAAGGGTTT+TGG + chr8_2:11362844-11362863 None:intergenic 35.0%
! TACTACTCATCACCTCTTTT+TGG - chr8_2:11362919-11362938 Msa1234140:intron 35.0%
AAGATGTTGAGTCCAAAAAG+AGG + chr8_2:11362934-11362953 None:intergenic 35.0%
! GAGACAACAATTTAGAACCA+TGG + chr8_2:11363010-11363029 None:intergenic 35.0%
GAGCTTGAGTAAGACTTAAA+GGG + chr8_2:11363330-11363349 None:intergenic 35.0%
TGAGCTTGAGTAAGACTTAA+AGG + chr8_2:11363331-11363350 None:intergenic 35.0%
GTCAACAAGAACAACAAACA+AGG - chr8_2:11363425-11363444 Msa1234140:intron 35.0%
TCACCAAAGAAATGATGAAG+TGG + chr8_2:11363477-11363496 None:intergenic 35.0%
TTCATCATTTCTTTGGTGAG+TGG - chr8_2:11363478-11363497 Msa1234140:intron 35.0%
CAATAGAAAAAACGGACAGA+CGG + chr8_2:11363682-11363701 None:intergenic 35.0%
ATCAAAATGTTATGTGTGGC+TGG - chr8_2:11363799-11363818 Msa1234140:five_prime_UTR 35.0%
TCAAAATGTTATGTGTGGCT+GGG - chr8_2:11363800-11363819 Msa1234140:five_prime_UTR 35.0%
GGAGATTATTGTACTGTGCA+TGG + chr8_2:11362082-11362101 None:intergenic 40.0%
! CAATACTTACTTGGTGGATG+AGG + chr8_2:11362362-11362381 None:intergenic 40.0%
ATAACTGTGAATGGTGCAGT+TGG - chr8_2:11362476-11362495 Msa1234140:CDS 40.0%
TAACTGTGAATGGTGCAGTT+GGG - chr8_2:11362477-11362496 Msa1234140:CDS 40.0%
GAAGAACAGATGGCAAGAAA+TGG - chr8_2:11362558-11362577 Msa1234140:CDS 40.0%
CAAGAAATGGAGATGCTCAA+AGG - chr8_2:11362571-11362590 Msa1234140:CDS 40.0%
CTTTCACAGTACTTTGAGTC+TGG + chr8_2:11362605-11362624 None:intergenic 40.0%
GAAGAAACTACTAGTTCCAC+AGG + chr8_2:11362662-11362681 None:intergenic 40.0%
GTAGAGAAAGGGAAAGAAGA+AGG + chr8_2:11362773-11362792 None:intergenic 40.0%
CCATGGAAGAAGTAGAGAAA+GGG + chr8_2:11362784-11362803 None:intergenic 40.0%
GAGTAGTAGCATCTTGAGTT+TGG + chr8_2:11362907-11362926 None:intergenic 40.0%
CTCATGATGACAAGGACTAT+AGG - chr8_2:11362957-11362976 Msa1234140:CDS 40.0%
TGATAAAGGAGGCAAGTTCA+AGG - chr8_2:11363201-11363220 Msa1234140:CDS 40.0%
GAGGCAAGTTCAAGGAATAA+GGG - chr8_2:11363209-11363228 Msa1234140:CDS 40.0%
CAAGTTCAAGGAATAAGGGA+TGG - chr8_2:11363213-11363232 Msa1234140:CDS 40.0%
ACTTTCTTTCCAGAAGCTAC+AGG - chr8_2:11363252-11363271 Msa1234140:CDS 40.0%
TAATCAACCTGTTGGAACTC+TGG + chr8_2:11363547-11363566 None:intergenic 40.0%
ATACTTTACGAGCATCCAGA+CGG - chr8_2:11363644-11363663 Msa1234140:CDS 40.0%
ATAACTTCACTCATCTGCCA+TGG + chr8_2:11363732-11363751 None:intergenic 40.0%
!! TTGATTAAGAGACTTGCTGG+TGG - chr8_2:11363755-11363774 Msa1234140:five_prime_UTR 40.0%
CAAAATGTTATGTGTGGCTG+GGG - chr8_2:11363801-11363820 Msa1234140:five_prime_UTR 40.0%
GGAGATTATTGTACTGTGCA+TGG + chr8_2:11362082-11362101 None:intergenic 40.0%
! CAATACTTACTTGGTGGATG+AGG + chr8_2:11362362-11362381 None:intergenic 40.0%
ATAACTGTGAATGGTGCAGT+TGG - chr8_2:11362476-11362495 Msa1234140:CDS 40.0%
TAACTGTGAATGGTGCAGTT+GGG - chr8_2:11362477-11362496 Msa1234140:CDS 40.0%
GAAGAACAGATGGCAAGAAA+TGG - chr8_2:11362558-11362577 Msa1234140:CDS 40.0%
CAAGAAATGGAGATGCTCAA+AGG - chr8_2:11362571-11362590 Msa1234140:CDS 40.0%
CTTTCACAGTACTTTGAGTC+TGG + chr8_2:11362605-11362624 None:intergenic 40.0%
GAAGAAACTACTAGTTCCAC+AGG + chr8_2:11362662-11362681 None:intergenic 40.0%
GTAGAGAAAGGGAAAGAAGA+AGG + chr8_2:11362773-11362792 None:intergenic 40.0%
CCATGGAAGAAGTAGAGAAA+GGG + chr8_2:11362784-11362803 None:intergenic 40.0%
GAGTAGTAGCATCTTGAGTT+TGG + chr8_2:11362907-11362926 None:intergenic 40.0%
CTCATGATGACAAGGACTAT+AGG - chr8_2:11362957-11362976 Msa1234140:CDS 40.0%
TGATAAAGGAGGCAAGTTCA+AGG - chr8_2:11363201-11363220 Msa1234140:CDS 40.0%
GAGGCAAGTTCAAGGAATAA+GGG - chr8_2:11363209-11363228 Msa1234140:CDS 40.0%
CAAGTTCAAGGAATAAGGGA+TGG - chr8_2:11363213-11363232 Msa1234140:CDS 40.0%
ACTTTCTTTCCAGAAGCTAC+AGG - chr8_2:11363252-11363271 Msa1234140:CDS 40.0%
TAATCAACCTGTTGGAACTC+TGG + chr8_2:11363547-11363566 None:intergenic 40.0%
ATACTTTACGAGCATCCAGA+CGG - chr8_2:11363644-11363663 Msa1234140:CDS 40.0%
ATAACTTCACTCATCTGCCA+TGG + chr8_2:11363732-11363751 None:intergenic 40.0%
!! TTGATTAAGAGACTTGCTGG+TGG - chr8_2:11363755-11363774 Msa1234140:five_prime_UTR 40.0%
CAAAATGTTATGTGTGGCTG+GGG - chr8_2:11363801-11363820 Msa1234140:five_prime_UTR 40.0%
TTAAGAGGGTTAAGAGGGGT+TGG + chr8_2:11362103-11362122 None:intergenic 45.0%
!!! TGGGTGGGGATGTTTTGAAA+TGG - chr8_2:11362496-11362515 Msa1234140:CDS 45.0%
!!! GGGTGGGGATGTTTTGAAAT+GGG - chr8_2:11362497-11362516 Msa1234140:CDS 45.0%
TGTGAAAGACACATGCACAG+AGG - chr8_2:11362617-11362636 Msa1234140:CDS 45.0%
AAACCGTTCAAGAAAGCCTG+TGG - chr8_2:11362643-11362662 Msa1234140:CDS 45.0%
GTTCCACAGGCTTTCTTGAA+CGG + chr8_2:11362649-11362668 None:intergenic 45.0%
! GCTGTTGTTGTTGTTCAAGG+AGG + chr8_2:11362738-11362757 None:intergenic 45.0%
! GCTGCTGTTGTTGTTGTTCA+AGG + chr8_2:11362741-11362760 None:intergenic 45.0%
CCCTTTCTCTACTTCTTCCA+TGG - chr8_2:11362781-11362800 Msa1234140:intron 45.0%
GCCATGGAAGAAGTAGAGAA+AGG + chr8_2:11362785-11362804 None:intergenic 45.0%
TGACTGATCATGACAAGCCA+TGG + chr8_2:11362801-11362820 None:intergenic 45.0%
GGAGGCAAGTTCAAGGAATA+AGG - chr8_2:11363208-11363227 Msa1234140:CDS 45.0%
AGGAATAAGGGATGGTACTG+TGG - chr8_2:11363221-11363240 Msa1234140:CDS 45.0%
CTAGATGAACCTGTAGCTTC+TGG + chr8_2:11363264-11363283 None:intergenic 45.0%
CAGAAGCTACAGGTTCATCT+AGG - chr8_2:11363262-11363281 Msa1234140:CDS 45.0%
CACCAAAGAACACAGATTCC+TGG - chr8_2:11363502-11363521 Msa1234140:intron 45.0%
AGCCAGGAATCTGTGTTCTT+TGG + chr8_2:11363507-11363526 None:intergenic 45.0%
TACTTTACGAGCATCCAGAC+GGG - chr8_2:11363645-11363664 Msa1234140:CDS 45.0%
GTTATGTAGGGGACCACATT+AGG - chr8_2:11363860-11363879 Msa1234140:five_prime_UTR 45.0%
TTAAGAGGGTTAAGAGGGGT+TGG + chr8_2:11362103-11362122 None:intergenic 45.0%
!!! TGGGTGGGGATGTTTTGAAA+TGG - chr8_2:11362496-11362515 Msa1234140:CDS 45.0%
!!! GGGTGGGGATGTTTTGAAAT+GGG - chr8_2:11362497-11362516 Msa1234140:CDS 45.0%
TGTGAAAGACACATGCACAG+AGG - chr8_2:11362617-11362636 Msa1234140:CDS 45.0%
AAACCGTTCAAGAAAGCCTG+TGG - chr8_2:11362643-11362662 Msa1234140:CDS 45.0%
GTTCCACAGGCTTTCTTGAA+CGG + chr8_2:11362649-11362668 None:intergenic 45.0%
! GCTGTTGTTGTTGTTCAAGG+AGG + chr8_2:11362738-11362757 None:intergenic 45.0%
! GCTGCTGTTGTTGTTGTTCA+AGG + chr8_2:11362741-11362760 None:intergenic 45.0%
CCCTTTCTCTACTTCTTCCA+TGG - chr8_2:11362781-11362800 Msa1234140:intron 45.0%
GCCATGGAAGAAGTAGAGAA+AGG + chr8_2:11362785-11362804 None:intergenic 45.0%
TGACTGATCATGACAAGCCA+TGG + chr8_2:11362801-11362820 None:intergenic 45.0%
GGAGGCAAGTTCAAGGAATA+AGG - chr8_2:11363208-11363227 Msa1234140:CDS 45.0%
AGGAATAAGGGATGGTACTG+TGG - chr8_2:11363221-11363240 Msa1234140:CDS 45.0%
CTAGATGAACCTGTAGCTTC+TGG + chr8_2:11363264-11363283 None:intergenic 45.0%
CAGAAGCTACAGGTTCATCT+AGG - chr8_2:11363262-11363281 Msa1234140:CDS 45.0%
CACCAAAGAACACAGATTCC+TGG - chr8_2:11363502-11363521 Msa1234140:intron 45.0%
AGCCAGGAATCTGTGTTCTT+TGG + chr8_2:11363507-11363526 None:intergenic 45.0%
TACTTTACGAGCATCCAGAC+GGG - chr8_2:11363645-11363664 Msa1234140:CDS 45.0%
GTTATGTAGGGGACCACATT+AGG - chr8_2:11363860-11363879 Msa1234140:five_prime_UTR 45.0%
!!! TTTTTAATATTTTATGAAAA+AGG - chr8_2:11362391-11362410 Msa1234140:CDS 5.0%
!! AATAATATTAAATAACTTTA+TGG + chr8_2:11363624-11363643 None:intergenic 5.0%
!!! TTTTTAATATTTTATGAAAA+AGG - chr8_2:11362391-11362410 Msa1234140:CDS 5.0%
!! AATAATATTAAATAACTTTA+TGG + chr8_2:11363624-11363643 None:intergenic 5.0%
TGTGAATGGTGCAGTTGGGT+GGG - chr8_2:11362481-11362500 Msa1234140:CDS 50.0%
AGAAAAGTAGACCCAGAGCC+AGG - chr8_2:11362527-11362546 Msa1234140:CDS 50.0%
GAAAAGTAGACCCAGAGCCA+GGG - chr8_2:11362528-11362547 Msa1234140:CDS 50.0%
GTTGGAAGCAAGATCTAGCC+AGG + chr8_2:11363523-11363542 None:intergenic 50.0%
TCCAACTCCAGAGTTCCAAC+AGG - chr8_2:11363537-11363556 Msa1234140:intron 50.0%
!! ACCTGTTGGAACTCTGGAGT+TGG + chr8_2:11363541-11363560 None:intergenic 50.0%
TGTGAATGGTGCAGTTGGGT+GGG - chr8_2:11362481-11362500 Msa1234140:CDS 50.0%
AGAAAAGTAGACCCAGAGCC+AGG - chr8_2:11362527-11362546 Msa1234140:CDS 50.0%
GAAAAGTAGACCCAGAGCCA+GGG - chr8_2:11362528-11362547 Msa1234140:CDS 50.0%
GTTGGAAGCAAGATCTAGCC+AGG + chr8_2:11363523-11363542 None:intergenic 50.0%
TCCAACTCCAGAGTTCCAAC+AGG - chr8_2:11363537-11363556 Msa1234140:intron 50.0%
!! ACCTGTTGGAACTCTGGAGT+TGG + chr8_2:11363541-11363560 None:intergenic 50.0%
CTGTGAATGGTGCAGTTGGG+TGG - chr8_2:11362480-11362499 Msa1234140:CDS 55.0%
GTGAATGGTGCAGTTGGGTG+GGG - chr8_2:11362482-11362501 Msa1234140:CDS 55.0%
TCTGTTCTTCTGCACCTCCC+TGG + chr8_2:11362548-11362567 None:intergenic 55.0%
! GGGAGGTGCAGAAGAACAGA+TGG - chr8_2:11362548-11362567 Msa1234140:CDS 55.0%
CTGTGAATGGTGCAGTTGGG+TGG - chr8_2:11362480-11362499 Msa1234140:CDS 55.0%
GTGAATGGTGCAGTTGGGTG+GGG - chr8_2:11362482-11362501 Msa1234140:CDS 55.0%
TCTGTTCTTCTGCACCTCCC+TGG + chr8_2:11362548-11362567 None:intergenic 55.0%
! GGGAGGTGCAGAAGAACAGA+TGG - chr8_2:11362548-11362567 Msa1234140:CDS 55.0%
AAGTAGACCCAGAGCCAGGG+AGG - chr8_2:11362531-11362550 Msa1234140:CDS 60.0%
! TTCTGCACCTCCCTGGCTCT+GGG + chr8_2:11362541-11362560 None:intergenic 60.0%
!! CGGACACAGAAGTACCCGTC+TGG + chr8_2:11363662-11363681 None:intergenic 60.0%
AAGTAGACCCAGAGCCAGGG+AGG - chr8_2:11362531-11362550 Msa1234140:CDS 60.0%
! TTCTGCACCTCCCTGGCTCT+GGG + chr8_2:11362541-11362560 None:intergenic 60.0%
!! CGGACACAGAAGTACCCGTC+TGG + chr8_2:11363662-11363681 None:intergenic 60.0%
CTTCTGCACCTCCCTGGCTC+TGG + chr8_2:11362542-11362561 None:intergenic 65.0%
CTTCTGCACCTCCCTGGCTC+TGG + chr8_2:11362542-11362561 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 11362039 11363889 11362039 ID=Msa1234140;Name=Msa1234140
chr8_2 mRNA 11362039 11363889 11362039 ID=Msa1234140-mRNA-1;Parent=Msa1234140;Name=Msa1234140-mRNA-1;_AED=0.05;_eAED=0.05;_QI=149|1|0.75|1|0.66|0.5|4|144|341
chr8_2 exon 11362039 11362190 11362039 ID=Msa1234140-mRNA-1:exon:4414;Parent=Msa1234140-mRNA-1
chr8_2 exon 11362370 11362719 11362370 ID=Msa1234140-mRNA-1:exon:4413;Parent=Msa1234140-mRNA-1
chr8_2 exon 11362950 11363433 11362950 ID=Msa1234140-mRNA-1:exon:4412;Parent=Msa1234140-mRNA-1
chr8_2 exon 11363557 11363889 11363557 ID=Msa1234140-mRNA-1:exon:4411;Parent=Msa1234140-mRNA-1
chr8_2 five_prime_UTR 11363741 11363889 11363741 ID=Msa1234140-mRNA-1:five_prime_utr;Parent=Msa1234140-mRNA-1
chr8_2 CDS 11363557 11363740 11363557 ID=Msa1234140-mRNA-1:cds;Parent=Msa1234140-mRNA-1
chr8_2 CDS 11362950 11363433 11362950 ID=Msa1234140-mRNA-1:cds;Parent=Msa1234140-mRNA-1
chr8_2 CDS 11362370 11362719 11362370 ID=Msa1234140-mRNA-1:cds;Parent=Msa1234140-mRNA-1
chr8_2 CDS 11362183 11362190 11362183 ID=Msa1234140-mRNA-1:cds;Parent=Msa1234140-mRNA-1
chr8_2 three_prime_UTR 11362039 11362182 11362039 ID=Msa1234140-mRNA-1:three_prime_utr;Parent=Msa1234140-mRNA-1
Gene Sequence

>Msa1234140

ATGATGAGTGCAAGTTCAAGAAATAGGTCTCTTTTCACACCAAATCAATGGCAAGAACTTGAACAACAAGCCCTAGTTTTTAAATACATGGTTACTGGAACACCTATTCCACCAGATCTCATATACTCTATTAAGAGAAGTTTAGACACTTCAATATCTTCAAGAATCTTTCCTCATCCACCAATTGGGTGGGGATGTTTTGAAATGGGATTTGGCAGAAAAGTAGACCCAGAGCCAGGGAGGTGCAGAAGAACAGATGGCAAGAAATGGAGATGCTCAAAGGAAGCATATCCAGACTCAAAGTACTGTGAAAGACACATGCACAGAGGTAGAAACCGTTCAAGAAAGCCTGTGGAACTAGTAGTTTCTTCTTCAACAACACCAACAAATAACACAAACACAGCATCTTCTTACAACAGAAACACCTCCTTGAACAACAACAACAGCAGCATAAACTCACCTTCTTCTTTCCCTTTCTCTACTTCTTCCATGGCTTGTCATGATCAGTCACAATCTTTTTCACAATCCTACCAAAACCCTTACTATTACTCTCAATCAATTACCTCTTCTAAAACACTTGATCATTCTGATTTCCAAACTCAAGATGCTACTACTCATCACCTCTTTTTGGACTCAACATCTTATTCTCATGATGACAAGGACTATAGGAGGCAAGTTCAAGGAATAAGGGATGGTACTGTGGATGAGAGAACTTTCTTTCCAGAAGCTACAGGTTCATCTAGGAGCTGCTATCATGATTCATATCAACAACAACTATCAATGAATCCCTTTAAGTCTTACTCAAGCTCACAGTTTCAAAATATCAATGATGATAATTCAAGACAACAAGAACAACACTGTTTTGTTTTAGGCACTGACATCAAGTCAACAAGAACAACAAACAAGGACAAAGAAAGTGAGACAACTCAGAAACCACTTCATCATTTCTTTGGTGAGTGGACACCAAAGAACACAGATTCCTGGCTAGATCTTGCTTCCAACTCCAGAGTTCCAACAGATGAGTGA
Protein sequence

>Msa1234140

MMSASSRNRSLFTPNQWQELEQQALVFKYMVTGTPIPPDLIYSIKRSLDTSISSRIFPHPPIGWGCFEMGFGRKVDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVELVVSSSTTPTNNTNTASSYNRNTSLNNNNSSINSPSSFPFSTSSMACHDQSQSFSQSYQNPYYYSQSITSSKTLDHSDFQTQDATTHHLFLDSTSYSHDDKDYRRQVQGIRDGTVDERTFFPEATGSSRSCYHDSYQQQLSMNPFKSYSSSQFQNINDDNSRQQEQHCFVLGTDIKSTRTTNKDKESETTQKPLHHFFGEWTPKNTDSWLDLASNSRVPTDE*